1331 results about "Ethanol precipitation" patented technology

The invention relates to a reconstructed tobacco coating liquid component-component group chemical kitchen combination, coupling preparation, and a coupling preparation process. Discarded waste tobacco leaves and stems are subjected to presoaking and enzymatic leaching or extraction to make tobacco leaf or stem enzymatically-leached / extracted solution, or tobacco leaf and stem mixed leached / extracted solution, certain components and component groups such as protein, pectin, chloride, nicotine, nitrosamines, nitrates and the like in the tobacco leaf and stem leached / extracted solution or tobacco leaf and stem leached / extracted mixture can be selectively converted, reduced or removed by combination of enzymolysis, Maillard reaction conversion, membrane separation / concentration or column separation, freeze separation, ethanol precipitation and the like, and the coupling preparation process for chemical components and component groups in the chemical kitchen, and accordingly, recombination or fitting, combination, regulation and coke reduction and harm reduction of the chemical components and component groups of the reconstructed tobacco coating liquid are achieved, quality of reconstructed tobacco is improved, and industrial technical integration and innovation on combination and coupling of the reconstructed tobacco coating liquid are realized.

The invention discloses a comprehensive extraction process of holothurian polypeptides and polysaccharides. The invention is characterized in that: fresh holothurians are taken as raw materials, gelatinized and hydrolyzed under the effect of composite trypsinases; precipitation and supernatant are respectively collected through centrifugation of the holothurians; ethanol is added into the supernatant for centrifugation; supernatant obtained is mixture of the holothurian polypeptides; centrifugal precipitations obtained for two times are synthesized, and A.S1398 purified neutral proteases are added for hydrolysis and centrifugation; ethanol is added into the supernatant for centrifugation; supernatant obtained for two times is synthesized into holothurian polypeptide mixture, and precipitation is crude polysaccharides; the holothurian polypeptide mixture is dissolved in water, and ultrafiltration membranes with different apertures are used for separation and purification of subsections of different molecular weights; holothurian polypeptide powders of different molecular weight sections are obtained after debitterizing, decolorization and freeze drying; crude polysaccharides are mixed into 5 percent aqueous solution; after centrifugation, decolorization and repeated centrifugation, precipitation is colleted and washed, and aqueous solution is mixed and dialyzed; ethanol is added for repeated precipitation for at least three times, and purified holothurian polysaccharides are obtained. The invention simultaneously prepares the holothurian polypeptides with different molecular weight sections and the holothurian polysaccharides, and has the advantages of high yield, high purity, high safety and mild reaction conditions.

The invention relates to a Chinese herbal medicinal bactericide, which aims to solve the problem of public hazards of the conventional bactericide. The bactericide comprises the following Chinese herbal medicines in part by weight: 40 to 120 parts of sessile stemona root, 40 to 120 parts of camphor tree, 35 to 105 parts of pomegranate rind, 35 to 105 parts of macleaya cordata, 35 to 105 parts of common andrographis herb, 35 to 105 parts of isatis root, 30 to 90 parts of barberry root, 30 to 90 parts of vietnamese sophora root, 30 to 90 parts of common fibraurea stem, 30 to 90 parts of densefruit pittany root-bark, 30 to 90 parts of officinal magnolia bark, 30 to 90 parts of common threewingnut root, 25 to 75 parts of akebia stem, 25 to 75 parts of oriental variegated coralbean bark, 25 to75 parts of fourstamen stephania root and 25 to 75 parts of humifuse euphorbia herb. The bactericide is prepared by the following steps of: performing ethanol precipitation on the Chinese herbal medicines to obtain the original liquid medicine; diluting sodium usnic acid by using a solvent; and adding 0.1 to 0.3 weight percent of diluted sodium usnic acid into the original liquid medicine. The finished Chinese herbal medicinal bactericide comprises 0.3 percent of stemonine, is brownish red, has the pH value of 7 to 8 and the density of 1.10g / cm<3>, and has the advantages of capability of effective sterilization and no damage to humans.

The invention provides a method for preparing biological flocculant by utilizing Bacillus licheniformis, which relates to a biological flocculant. The invention provides a method for preparing biological flocculant by utilizing Bacillus licheniformis, which has high flocculation activity, low cost for raw materials and great industrial application potential. A microorganism is Bacillus licheniformis. The method comprises the following steps that: lawn on a fresh inclined plane is transferred to a seed culture medium for culture and then transferred to a fermentation culture medium for culture so as to obtain biological-flocculant fermentation broth; the biological-flocculant fermentation broth is centrifuged to remove precipitate and collect supernatant; the supernatant is added with ethanol, kept to stand, centrifuged and then removed; the precipitate is added with absolute ethanol, stirred and centrifuged to remove the supernatant; ethanol precipitate is added with hexadecyl trimethyl ammonium bromide and centrifuged to remove the supernatant so as to obtain the precipitate; the precipitate is dissolved in a NaCl solution, added with the absolute ethanol, stirred, stood and centrifuged to remove the supernatant so as to obtain the precipitate; and the precipitate is frozen and dried in vacuum so as to obtain pure biological flocculant.

The invention discloses a method for extracting fruit dreg dietary fiber through high-pressure microfluidization ultramicro crushing and enzymolysis coupling and belongs to the technical field of agricultural and sideline product waste deep processing. According to the method, water is added into peach dreg for dispersing the peach dreg, the pH is regulated, high-temperature-resistance alpha-amylase and protease are respectively used for enzymolysis for eliminating starch and protein, the peach dreg subjected to the enzymolysis is treated by a high-speed shearing instrument and is subjected to high-pressure microfluidization ultramicro crushing, then, cellulose is added for enzymolysis and centrifugation, filter liquid after enzymolysis and precipitates after enzymolysis are respectively obtained, the filter liquid is subjected to concentration, ethanol precipitation and vacuum drying to obtain high-activity water-soluble dietary fiber, and the precipitates are subjected to water washing and vacuum drying to obtain high-purity insoluble dietary fiber. Waste fruit dreg after the juice squeezing is utilized as raw materials, the fine and deep processing of agricultural and sideline products is realized, and the resource waste is reduced.

Disclosed is a process for extracting tea polyphenol, caffeine as a byproduct thereof and tea polysaccharide from tea, which comprises, (1) extracting active ingredients including tea polyphenol, caffeine and tea polysaccharide from the tea through composite enzymolysis abstraction and water abstraction, (2) filtering the raffinate with hyperfiltration membrane, (3) subjecting the membrane compression liquid to ethanol deposition so as to obtain crude polysaccharides, removing protein and carrying out ethanol deposition, drying to obtain the tea polysaccharides, (4) separating and purifying through second level resin adsorption chromatography, thus obtaining tea polyphenol as well as caffeine as by-product.

This inventnion relates to a preparation method for high-efficiency prebiotics oat beta-glucan, belonging to the quintessential processing technologies of oat. The said method includes using oat bran as raw materials, crushing, microwave-assisted-extracting, adding amylase and glucoamylase, isoelectric-point-precipitating, centrifugating and separating, concentrating the supernatant, precipitating with ethanol, centrifugating and collecting the precipitation, solving with water, hydrolyzing with beta-glucanase, and spray-drying process to obtain the high-efficiency prebiotics oat beta-glucan with significantly enhanced intestinal and fecal bifidobacteria and actobacillus value-added effect. The method is scientifically and rationally designed, and of great significance in achieving the comprehensive utilization of resources oat and increasing its added value.

The invention relates to a simple method for preparing L-fucoidan by a kind of sea-tangle from Qingdao as materials which comprises: extracting and purifying the said sea-tangle by the water extract and ethanol precipitation to get purified fucoidan sulfate, then hydrolyzing sulphuric acid, filtering and the filtrate desalting through a column, deoxidizing and decolouring, filtering and the filter liquor rotating and concentrating in vacuum, alcohol depositing, the filter liquor rotating and concentrating to get syrup in vacuum, alcohol depositing, the filter liquor rotating and concentrating to get syrup in vacuum, adding the right amount mixture of alcohol and acetone, and adding several L-fucoidan particles to standard seed and crystal. The production purity is 98.1 %, and the productivity is 16.2-17.0 % in the invention.

The invention discloses an enteromorpha polysaccharide having immunocompetence and a preparation method thereof. The enteromorpha polysaccharide having immunocompetence is a polysaccharide HT-II. The enteromorpha polysaccharide having immunocompetence is a white floccule, can be well dissolved in water and dimethyl sulfoxide, has relative molecular weight of 96kDa, and mainly comprises glucose and mannose, wherein a ratio of rhamnoside to xylose to mannoside to glucose is 36: 20: 6: 38. The preparation method comprises the following steps of breaking down, carrying out extraction at a temperature of 90 DEG C by water, filtrating, merging the extract, concentrating, carrying out precipitation by ethanol, removing proteins, carrying out separation and purification, and carrying out freeze drying to obtain the purified enteromorpha polysaccharide having immunocompetence. The preparation method is simple, has stable performances, a low cost and a large medical value, can reduce cholesterol content and can improve immunity. The preparation method adopts extraction, protein removal and purification processes, and scientific experimental means, is a novel method for controlling enteromorpha, and provides a novel approach for controlling enteromorpha. The preparation method of the enteromorpha polysaccharide having immunocompetence develops enteromorpha medical values and purposes, controls environmental pollution, reduces a clean-up cost, improves economic benefits and is an ideal special preparation technology for enteromorpha control and integrated utilization.

The invention relates to Chinese herbal tea and a preparation method thereof, and in particular relates to a concentrated solution of Chinese herbal tea and a preparation method thereof. The herbal tea comprises 1000-3500 parts of Chinese mesona herb, 135-900 parts of frangipani, 45-900 parts of folium microcotis, 180-900 parts of chrysanthemum, 180-900 parts of honeysuckle, 180-900 parts of common selfheal fruit-spike and 45-900 parts of liquorice by weight. After raw material treatment, aqueous extraction, ethanol precipitation, concentration, water precipitation, centrifugation and sterilizing packaging, the concentrated solution the concentration of which is 30-35Be is obtained. The utilization rate of active ingredients of Chinese herbal medicines is increased by adopting the preparation method and the portable and healthy herbal tea drink is obtained.

The invention discloses fuscoporia obliqua active ingredients capable of lowering blood sugar and a preparation method and application of the fuscoporia obliqua active ingredients. The preparation method takes fuscoporia obliqua fruit body as raw material and comprises the following steps: respectively extracting, filtering and concentrating the fuscoporia obliqua fruit body with normal temperature water and high temperature water; adding alcohol into concentrate and depositing to obtain crude polysaccharide; respectively pouring the polysaccharide extracted with normal temperature water and the crude polysaccharide extracted with high temperature water to flow through a (diethylaminoethanol) DEAE-52 cellulose column; carrying out subsection elution by using distilled water and NaCl solutions with different concentrations; and collecting stepwise elution peak sugar solution. Internal blood sugar reduction activity experiment shows that 0.2mol / L NaCl-section eluted sugar of the crude polysaccharide extracted with normal temperature water and 0.2mol / L NaCl-section eluted sugar of the crude polysaccharide extracted with high temperature water both have obvious blood sugar reduction activity, same blood sugar reduction activity with the blood sugar reduction medicine of metformin hydrochloride, and no obvious toxic or side effect.

The invention discloses an extraction and purification process for medicinal components of Scrophulariaceae, namely an extraction and purification process for iridoid and phenylpropanoid components: pulverizing dry roots of Scrophulariaceae, soaking, water extraction, concentration of the extract, and ethanol precipitation , resin adsorption, ethanol elution, concentrated drying and other procedures obtained. The extraction and purification process of the invention has high extraction rate, can reach more than 50%, stable and controllable quality, simple method and low composition, and is suitable for large-scale industrial production, thereby providing a new way for the development and utilization of Scrophulariaceae.

The invention discloses a human cytomegalovirus immunoglobulin for intravenous injection and a preparation method thereof, and aims to improve the purity, yield and safety of the product. In the invention, the specific activity of the human cytomegalovirus immunoglobulin for intravenous injection is not less than 2.5 PEI-U / mg, the anti-CMV titer is not less than 100 PEI-U / ml, the purity is greater than 98.2%, and the protein content is 51-55 mg / ml. Caprylic acid precipitation and anion exchange chromatography are used instead of the partial ethanol precipitation step in the traditional low-temperature ethanol method, thereby keeping IgG in the supernate all the time so as to keep the IgG activity; and processes of caprylic acid virus inactivation and nano film virus removal are used, thereby effectively ensuring the safety of the product. Researches show that the preparation method disclosed by the invention improves the purity, yield and safety of the product, saves the energy and reduces the production cost.

The present invention discloses a preparation method of ganoderma lucidum polysaccharide, which belongs to the field of biological medicine. The fruiting body of ganoderma lucidum is baked and ground until ganoderma lucidum powder is produced; the ground ganoderma lucidum powder is uniformly mixed with pure water which is twenty eight times more than the ganoderma lucidum powder, and smashed by a 680W ultrasonic device for seventeen minutes; after extraction, filtration and dreg removing, the extract is rotationally evaporated until one quarter of the original volume; 95 percent ethanol, the volume of which is four times larger than the volume of the concentrated solution, is slowly added into the concentrated solution; after twelve hours of ethanol precipitation under a temperature equal to 4 DEG C and fifteen minutes of centrifugation at 4500rpm, supernatant is removed; and after being washed respectively by absolute ethyl alcohol, acetone and ether, precipitate is baked in an oven under a temperature equal to 55 DEG C in order to obtain the crude ganoderma lucidum polysaccharide. Since the ultrasonic extraction technique is adopted, the yield can reach 20.79 plus or minus 0.11mg / g, purity can reach 47.88 percent, and compared with the conventional hot water soaking extraction, the time is shorten by 96 percent while the yield is increased by 28 percent. The obtained ganoderma lucidum polysaccharide can be widely used as the base of drugs and health-caring food.

A process for preparing the sodium hyaluronate from the flermented liquid of hyaluronic acid incldues such steps as removing bacteria from said fermented liquid, enzymolyzing, depositing in alcohol, complexing, cleavaging, depositing in alcohol, ultrafiltering, depositing in alcohol, washing, dewatering and dryin. It has high molecular weight and low content of protein.

The invention relates to a chemical extraction technology, and especially relates to a method for extracting pectin from tobacco. The method comprises specific steps that: tobacco is crushed, such that tobacco powder is obtained; the tobacco powder is soaked by using water for at least 8 hours, and is processed through steam explosion for at least 60 seconds under a pressure of 0.04 to 0.3Mpa, such that a tobacco extract is obtained; an acid is added to the tobacco extract, and a pH value of the solution is regulated to 1.0 to 3.0; the solution is completely hydrolyzed under a temperature of 70 to 90 DEG C; residues are removed, such that a hydrolysate is obtained; the hydrolysate obtained in the step A is precipitated by using a ethanol precipitation method, and the obtained precipitate is pectin. Compared to a traditional method wherein a tobacco material is boiled by using boiled water, and wherein pectinase is passivated, the tobacco extract preparation method provided by the invention is advantaged in low pollution, low energy consumption, and complete pectinase passivation. With themethod provided by the invention, 1 hour can be saved, and the yield of pectin can reach approximately 12.2%, which is increased by 3.2% comparing to that of a traditional ethanol extraction method. Meanwhile, the color of the obtained pectin is good.

The invention discloses a method for extracting rice bran polysaccharide from sub-critical water, belonging to the technical field of separation of active components of natural products. The method comprises the following steps of: crushing the degreased rice bran and sieving with a screen with 60 meshes; feeding the sieved rice bran into an extraction kettle; pumping water into the extraction kettle; controlling the pressure of the extraction kettle to be 5-20MPa, the extraction temperature to be 100-200DEG C and the extraction time to be 5-60 minutes; cooling and filtering; removing protein with trichloroacetic acid; centrifuging and precipitating supernate with ethanol; collecting the precipitation, freezing and drying to obtain rice bran polysaccharide. The rice bran polysaccharide has the advantages of high extraction yield, low energy consumption, cleanness of process, environment friendliness and simple process. The invention is more convenient for researching the structure and functions of the rice bran polysaccharide and developing products with high added value of the rice bran polysaccharide.

The invention discloses a novel method for preparing wheat bran dietary fibers by taking wheat bran as a raw material. The wheat bran dietary fibers are dietary fibers enriched with araboxylan and the content is more than 16.08%. The novel method takes the wheat bran as the raw material and sequentially comprises the following process steps: pre-treating the raw material; rapidly extracting with an alkaline solution; carrying out ethanol precipitation; adding water to suspend again and blasting by steam; hydrolyzing compound protease; hydrolyzing compound amylase; carrying out secondary alcohol precipitation; and sieving. The dietary fiber product has high wheat bran dietary fiber yield and dietary fiber purity, and also has high content of araboxylan and high content of soluble dietary fibers; the product has good functional properties including water binding capacity, viscosity and the like, and has a plurality of physiological functions of relaxing bowel, controlling blood glucose, lowering blood fat, adjusting human immunity and preventing colon cancer and the like, so that high-quality raw material guarantees are provided for widely applying the wheat bran dietary fibers to foods.

The invention discloses a method for separating hemicellulose from high-production-yield waste pulping and an application thereof. The method comprises the steps of regulating the pH value of the waste pulping to 3 by a concentrated sulfuric acid under room temperture, standing for 30min in a water bath under the constant temperature of 45 DEG C, depositing lignin and carrying out extraction and filtering, neutralizing a filtrate to reach a pH value of 5 by 6mol / L sodium hydroxide, depositing with alcohol with 3 times of volume, filtering, washing by the alcohol and drying in vacuume till constant weight so as to obtain the hemicellulose. To be taken as an internal intensifier, the hemicellulose obtained from separation is modified by quaternary ammonium salt cation. The quaternary ammonium salt cation hemicellulose is taken as the internal intensifier for broadleaf pulp, conifer pulp and waste OCC pulp and reaches good intensification effect. The method has the advantages of simple technology, easy operation and lower processing cost and can reach the purpose of 'changing waste into treasures'.

The invention provides a polymerase chain reaction-sequence based typing (PCR-SBT) method for ABO blood type genotyping. The method comprises the following steps of: preparing human genome DNA; amplifying segments of ABO gene exon 1, exons 2-4 and exons 5-7; performing double enzyme digestion purification on the obtained amplified products; performing a sequencing PCR reaction on the purified products; purifying the sequenced products by a sodium acetate-ethanol precipitation method and performing capillary electrophoresis sequencing; and analyzing the obtained sequences by using software to determine the genotype. The method has the advantages of solving the problems of identification of an ABO subtype, judgment of difficult blood types, discovery of a new mutational site, gene recombination among genes, genetic polymorphism detection and the like, exerting the characteristics of high flux and result accuracy of ABO genotyping operation by PCR-SBT, achieving great importance for the relative application in the fields of clinical transfusion medicinal research, genetics and the like and having important practical significance for medicinal research units, pharmic research and reagent development units.

The invention discloses a method for comprehensively extracting tea seed saponin, tea seed polypeptide and tea seed polysaccharide by using tea seed cake as a raw material. The extracting method comprises the steps of mixing degreased tea seed cake with ethanol, treating with a colloid mill and a homogenizer, then performing water bath ethanol extraction, centrifuging, performing ultra-filtration, and concentrating to obtain the tea seed saponin; mixing the centrifuged sediment with water, extracting with ultrasonic and microwave, centrifuging, regulating the pH of the supernate, centrifuging for the second time, dissolving the sediment, and performing enzymolysis, enzyme deactivation, ultra-filtration and spraying to obtain the tea seed polypeptide; and spraying the supernate obtained by secondary centrifuging to obtain coarse tea seed polysaccharide, and then performing ultra-filtration and ethanol precipitation to obtain the high-purity tea seed polysaccharide. The method is simple, low in production and equipment cost, high in product extraction rate and high in purity, and greatly improves the additional value of the tea seed cake. A new thought and a new method are provided for efficient comprehensive utilization of the tea seed cake.

The invention relates to a method for comprehensively using viscera of a sea cucumber, particularly relates to a method for extracting sea cucumber polysaccharide and sea cucumber polypeptide from the viscera of the sea cucumber through a biological enzymolysis technology and a membrane separation technology. The method comprises the following main steps of: 1, pre-treatment; 2, alkali extraction; 3, enzymolysis, 4, centrifugal separation; 5, microfiltration membrane impurity removal; 6, nonefiltration membrane concentration; 7, ethanol precipitation; 8, centrifugal separation; and 9, drying for obtaining sea cucumber polysaccharide and sea cucumber polypeptide respectively. The technical scheme provided by the invention is simple and easy in process and is applicable to industrial production. The extraction rate of protein and total sugar which are contained in viscera of sea cucumber can reach 60-70%. The effective active ingredients in the target products have higher contents. Furthermore, no residual solvent exists, and the security is high.

The invention relates to a method for integrally extracting steroidal compounds, polysaccharides and polyphenols from inonotus obliquus. The method mainly comprises the following steps: high-pressure treatment, ethanol extraction, concentration, centrifugation, deionized water extraction, concentration, ethanol precipitation, separation, purification, freezing and drying. Compared with the conventional extraction method, the provided method has the biggest difference in that high-pressure treatment is carried out to the raw material for preliminary treatment under the appropriate time condition, then an extract containing steroids is obtained by an ethanol extraction method, the steroidal compounds are purified by a water washing method and a centrifugation method, extracts of polysaccharides and polyphenols are obtained by a water extraction method, and then the polysaccharides and polyphenols are separated through ethanol precipitation. The tissue structure of inonotus obliquus changes due to high-pressure treatment, therefore, the yield of the steroidal compounds, polysaccharides and polyphenols is high, the molecular structure of active components in inonotus obliquus changes under high pressure, and the study verifies that the activity of inonotus obliquus can be enhanced.

The invention discloses a method for simultaneously extracting soybean peptide and soybean oligosaccharide from an aqueous phase produced through the aqueous enzymatic method and belongs to the technology of gain and oil bioprocessing. The method comprises the following steps: (1) soybeans are extruded and puffed, and then mixed with water for enzymolysis, and centrifugal separation is performed after enzymolysis, so that free oil, an emulsion, a hydrolysate and residues are obtained; (2) the emulsion, the hydrolysate and the residues are subjected to ultrasonic treatment to obtain a mixed liquor, two types of alkaline protease are added into the mixed liquor for step-by-step enzymolysis, centrifugal separation is performed after enzymolysis, so that free oil, an aqueous phase waste liquor and residues are obtained; (3) the acid deposition is performed on the aqueous phase waste liquor and then centrifugal separation is performed on the aqueous phase waste liquor to obtain sediment and aqueous phase mixture, and the sediment is subjected to vacuum concentration and spray drying to obtain soybean peptide; nanofiltration and ethanol precipitation are performed on the aqueous phase mixture to obtain sediment, and the sediment is subjected to decolorization and desalination treatment, and then subjected to vacuum concentration and spray drying to obtain the soybean oligosaccharide. According to the method, the aqueous phase mixed system formed during oil production through the aqueous enzymatic method is fully utilized, so that the waste is reduced, and the soybean peptide and the soybean oligosaccharide can be simultaneously obtained; the method has an excellent application prospect.

The invention discloses bletilla polysaccharide, a preparation method and novel application thereof. The bletilla polysaccharide is prepared by the following method: firstly, coarse bletilla powder is taken as a raw material, and is extracted by alcohol; secondly, herb residue is extracted by water; thirdly, resin is used for removing proteins of a concentrated solution; and fourthly, after an eluent is condensed, the bletilla polysaccharide is precipitated by the ethanol. The content of the bletilla polysaccharide prepared by the method is high, reaches 70 to 74 percent, and is improved by 14 to 20 percent compared with the prior technical method, and the bletilla polysaccharide has good protein removal effect. The preparation method can also utilize the herb residue produced in the process of production of bletilla particle medicines as the raw material, so as to save the production cost. The invention also provides the novel application of the bletilla polysaccharide to preparation of medicines for treating gastric ulcer and medicines for treating silicosis and a commonly used medical preparation formulation.

The invention provides a method for efficiently extracting lycium barbarum polysaccharide. The method comprises the steps: adding lycium barbarum into an extraction kettle, injecting preheated deionized water to the extraction kettle at the flow speed of about 70mL / min by using a pump, controlling the liquid to solid ratio to be 10:1 to 30:1mL / g, simultaneously extracting the lycium barbarum polysaccharide at an extraction phase by using synergistic ultrasonic and subcritical water methods after heating to the preset temperature (larger than or equal to 100 DEG C), controlling the extraction pressure to 3-7MPa, the extraction temperature to 100-130 DEG C and the extraction time within 20-80min, and discharging an extracting solution after the extraction is ended, wherein the lycium barbarum is dried to the constant weight, the frequency of the ultrasonic is 18.5Hz, and the ultrasonic power is 100-140W; and after the extracting solution is filtered and subjected to vacuum concentration, settling for 12h by using 95% ethanol, removing a supernatant liquid and drying in vacuum to obtain the lycium barbarum polysaccharide. The yield of the lycium barbarum polysaccharide extracted by using the method is higher than the yields of lycium barbarum polysaccharides extracted by using a hot water extraction method, an ultrasonic extraction method and a subcritical water extraction method, and the antioxidant activity of the lycium barbarum polysaccharide extracted by using the method is stronger than the antioxidant activities of the lycium barbarum polysaccharides obtained by using the hot water extraction method, the ultrasonic extraction method and the subcritical water extraction method. Therefore, the method can lay the foundation for the industrial production and pharmacological research of the lycium barbarum polysaccharide.

The invention discloses a fermentation production method of sialic acid and belongs to the technical field of bioengineering. The method comprises the following steps: adding a seed liquid into a fermentation tank containing a sterilizing fermentation culture medium; continuously fermenting to produce fermentation liquid containing polysialic acid in a way of replenishing feeding liquid and a hydrogen dioxide solution; then carrying out centrifugal separation, ethanol precipitation and filtration to obtain refined polysialic acid; then, carrying out acidolysis and crystallization on the polysialic acid; and finally, washing and freeze-drying to obtain sialic acid powder. The output of the polysialic acid produced by the method provided by the invention can reach 12.96g / L, the already reported maximum content of the polysialic acid is improved by 87.28%, the hydrolysis rate of polysialic acid is 95%, the content of purified sialic acid reaches 10.01g / L and the purity reaches 94.80%. The method is suitable for industrial promotion and application.

The invention discloses an enzymatic extraction method for auricularia auricula polysaccharides, which comprises the following steps of: mechanically and coarsely grinding dried auricularia auricula, sieving, mixing according to a material-to-water ratio of 1:(50-200), and extracting at the temperature of between 35 and 50 DEG C and the pH of between 5.0 and 9.0 under the action of chitinase for 60 to 180 minutes, wherein the addition amount of the chitinase is 100 to 600U / g; raising the extraction temperature to be between 80 and 95 DEG C, extracting for 60 to 180 minutes to obtain extract, and centrifuging to obtain supernatant and precipitates; and adding ethanol into the supernatant to precipitate polysaccharides, performing centrifugal separation to obtain precipitates, dissolving the precipitates in water, concentrating to remove residual ethanol, adding a mixture of diatomite and activated carbon into concentrated solution, mixing, fully stirring, filtering, performing membrane filtration on filtrate by using a polysulfone membrane of 30 to 100kD, collecting macromolecular concentrated solution which does not filter through the membrane, and drying the macromolecular concentrated solution to obtain the auricularia auricula polysaccharides. The prepared products have high purity and bioactivity; and the polysaccharide content is over 40 percent.

The invention discloses a ganoderan extraction method and a ganoderan use. The ganoderan extraction method utilizes an ultrasonic enzyme method to realize ganoderan extraction and comprises ganoderma lucidum crushing, extraction, ultrafiltration, condensation and ethanol precipitation separation. The ganoderan extraction method has a ganoderan extraction rate of 3.14 to 4.21%. The ganoderan obtained by the ganoderan extraction method has molecular weight less than 10000 dalton and is light brown. The ganoderan extraction method has simple extraction separation processes and is suitable for industrial production. Ganoderan has strong antioxidant activity and anticancer activity. In a DPPH. free radical system, IC50 of ganoderan is in a range of 0.28 to 0.43mg / mL and an ORAC value of ganoderan is in a range of 1207.66 to 1777.90 micromole Trolox / g. When a concentration of ganoderan is 2mg / ml, ganoderan has a human lung cancer cell A549 inhibition rate of 53.04 to 68.37 and can be used for preparation of an anticancer drug.