267 results about "Ethanol precipitation" patented technology

The invention relates to the technical field of plant polysaccharide extraction, in particular to a process for synergistically extracting rape pollen polysaccharide by a compound enzyme, which comprises the following steps: crushing rape pollen to 200-300 meshes by pulse airflow, performing microwave-ultrasonic degreasing by using a 85% ethanol solution, mixing with an acetic acid-sodium acetate buffer solution with the pH value of 4.8, and performing ultrasonic extraction to obtain a crude extract; the method comprises the following steps: adding a compound enzyme system which is immobilized by Fe3O4 (at) SiO2 nanoparticles by cellulase, pectinase, protease and beta-glucosidase according to a mass ratio of 2: 1: 1: 0.5, carrying out enzymolysis for 4 hours in a 50 DEG C constant-temperature water bath and a 15V / cm pulsed electric field, carrying out enzyme deactivation on enzymatic hydrolysate, carrying out gradient ethanol precipitation, carrying out ultrafiltration deproteinization, and carrying out vacuum pulsation freeze drying to obtain the polysaccharide. According to the method disclosed by the invention, the cell wall breakage rate and the enzymolysis efficiency are improved through pulsed airflow crushing, magnetic nano immobilized enzyme and pulsed electric field assistance, the polysaccharide yield reaches 7.32%, the purity is 91%, the beta-glucosidic bond proportion and the antioxidant activity are remarkably improved, the enzyme can be recycled and reused, the drying time is shortened, and efficient and low-consumption extraction is realized.

The present invention discloses an anti-non-small cell lung cancer active birch inonotus polysaccharide IOP-1 and its application, belonging to the field of biomedicine. The present invention adopts a method of boiling water extraction and ethanol precipitation of birch inonotus polysaccharide, and combines DEAE cellulose column purification technology, and utilizes the principle that macromolecular polysaccharides are insoluble in organic solvents to successfully achieve the separation of birch inonotus polysaccharide and obtain a birch inonotus polysaccharide IOP-1 with anti-non-small cell lung cancer activity, wherein the monosaccharide composition of the birch inonotus polysaccharide IOP-1 includes fucose, rhamnose, arabinose, galactose, glucose, xylose and mannose with a molar mass ratio of 2.41:0.58:1.03:2.17:82.20:4.33:7.29. Its significant effect against non-small cell lung cancer was verified by in vitro and in vivo experiments, providing a new treatment method for the clinical treatment of non-small cell lung cancer.

The invention discloses a method for improving the conversion efficiency of insoluble dietary fibers and soluble dietary fibers obtained by the method, and belongs to the technical field of food processing and functional ingredient modification. The method comprises the following steps: performing superfine grinding treatment on wheat bran to obtain wheat bran powder; adding water into wheat bran powder, and then carrying out high-temperature and high-pressure treatment to obtain a wheat bran solution; then carrying out stepped enzymolysis on the wheat bran solution to obtain enzymatic hydrolysate; the enzymatic hydrolysate is centrifuged, lower-layer solid precipitates are insoluble dietary fibers, supernate is subjected to ethanol precipitation, and the soluble dietary fibers are obtained. The wheat bran is modified by combining superfine grinding and high-temperature and high-pressure pretreatment with stepped enzymolysis, so that the yield of the soluble dietary fibers is remarkably increased, and the functional characteristics of the soluble dietary fibers are improved. The obtained soluble dietary fiber has excellent physicochemical properties and physiological functions, and can be widely applied to baked foods, beverages, dietary supplements and other products, so that the soluble dietary fiber has important application prospects and value in the technical field of food processing.

The invention discloses application of uncaria acidic polysaccharide URP-2 in preparation of an anti-esophageal cancer drug, and belongs to the technical field of antitumor of medicinal plant polysaccharide. According to the method, a method for extracting the uncaria total polysaccharide by boiling water and precipitating the uncaria total polysaccharide by ethanol is adopted, and a DEAE DE-52 cellulose column and sephadex column purification technology is combined, so that the separation of the uncaria acidic polysaccharide component is realized, and the uncaria acidic polysaccharide URP-2 is obtained. The uncaria acidic polysaccharide URP-2 comprises the following components in mole percent: 14.11% of arabinose, 12.64% of rhamnose, 6.48% of galactose, 5.00% of glucose and 61.77% of galacturonic acid. Experiments show that the uncaria acidic polysaccharide URP-2 has remarkable anti-esophageal cancer activity, so that a potential experimental basis and a new strategy are provided for applying the uncaria acidic polysaccharide URP-2 to clinical treatment of esophageal cancer.

The invention discloses a digestion-aiding highland barley fried malt polysaccharide as well as a preparation method and application thereof. The polysaccharide is extracted from highland barley through hot water extraction, ethanol precipitation, deproteinization, ion exchange column chromatography, gel column chromatography and dialysis processes. Animal experiments show that the polysaccharide can significantly improve the small intestine propulsion rate of mice and increase the weight increment, gastric juice amount, pepsin activity and pepsin discharge of rats, and the effect of the polysaccharide is superior to that of traditional roasted barley malt polysaccharide; the highland barley polysaccharide is superior to highland barley crude polysaccharide, highland barley polyphenol, highland barley lipid, highland barley protein and the like. The invention has the advantages of easily available raw materials and green process, is suitable for developing side-effect-free digestion-aiding functional foods such as tea bags, milk tea, yoghurt and biscuits, and has important market value.

The invention relates to the technical field of water treatment, in particular to a method for preparing a quaternized modified tannin-based organic flocculant through irradiation grafting, a product prepared through the method and application of the quaternized modified tannin-based organic flocculant. The method comprises the following steps: (1) under the irradiation of 30-60 mW / cm < 2 > of ultraviolet light, adding brominated vinyl triphenylphosphine into a mixture of tannic acid and a photoinitiator for reaction; (2) adjusting the ultraviolet intensity to 5-20 mW / cm < 2 > for reaction; (3) closing the ultraviolet reaction; and (4) precipitating the product with ethanol, filtering and washing to obtain the quaternary phosphonium modified tannin-based organic flocculant. Wherein the photoinitiator is selected from one of benzophenone and benzoin dimethyl ether. The quaternized modified tannin-based organic flocculant provided by the invention can efficiently remove algae in a water body in an environment-friendly manner, and avoids secondary pollution caused by algae cell rupture at the same time.

The invention relates to the technical field of plant polysaccharides, and particularly discloses a red-knees herb polysaccharide and a preparation method and application thereof, the peak top molecular weight of the red-knees herb polysaccharide is 33.9 KDa, the weight-average molecular weight of the red-knees herb polysaccharide is 42.8 KDa, the number-average molecular weight of the red-knees herb polysaccharide is 10.0 KDa, and the red-knees herb polysaccharide is mainly composed of galacturonic acid, galactose, arabinose, rhamnose and glucuronic acid. According to the preparation method of the red-knees herb polysaccharide, by optimizing process parameters and controlling the reasonable solid-liquid ratio, heating temperature and heating time, the dissolution rate of the polysaccharide is increased, the dissolution rate of impurities is reduced, and the yield of the red-knees herb polysaccharide is increased. The red-knees herb polysaccharide is used as a raw material, ethanol is used for alcohol precipitation, polysaccharide components are precipitated, impurities are preliminarily removed, then macroporous resin is used for purification, the recovery rate of polysaccharide and the removal rate of impurities such as pigment and protein are improved by screening optimization and pretreatment of the macroporous resin, and the total sugar content of the purified red-knees herb polysaccharide is 80% or above. Use of toxic chemical reagents is avoided, and the preparation method is safe and efficient.

The invention discloses a degradable nanocellulose sustained-release drug-loaded film and a preparation method thereof, and belongs to the technical field of drug functional materials. The preparation method comprises the following steps: mixing and stirring microcrystalline cellulose and 64wt% sulfuric acid solution, centrifuging, dialyzing to be neutral, and freeze-drying to obtain CNCs; cNCs, NaIO4 and water are mixed and then subjected to a light-shielding reaction, ethanol precipitation is added, dialysis purification is conducted, freeze drying is conducted, and formylation modified CNCs are obtained; mixing and stirring the aldehyde modified CNCs, sericin, polyvinyl alcohol, water and a target drug to obtain a core layer solution; dissolving a polylactic acid-glycolic acid copolymer into a mixed solution of chloroform and DMF (Dimethyl Formamide) to obtain a shell solution; the core layer solution and the shell layer solution are subjected to coaxial electrostatic spinning forming to obtain the drug-loaded fiber, then the drug-loaded fiber is subjected to biomimetic mineralization surface modification to obtain the degradable nanocellulose sustained-release drug-loaded film, the drug loading rate is increased, the mechanical property of the drug-loaded film is improved, and the sustained-release and release-controllable effects are achieved.

The invention discloses a sea buckthorn fruit water-soluble micromolecule and a component extraction process thereof, and relates to the technical field of natural plant chemistry. The method comprises the following steps: S1, pretreating the sea buckthorn fruits, namely crushing the sea buckthorn fruits, accurately weighing the crushed sea buckthorn fruits, adding ultrapure water according to a ratio of 1g: 10mL, carrying out hot water extraction and centrifugation, and filtering impurities by using a filter membrane; s2, extracting sea buckthorn fruit water-soluble small molecules, adding high-concentration absolute ethyl alcohol into centrifugally filtered supernate, sealing and standing overnight at 4 DEG C, centrifuging an alcohol precipitation sample, filtering, performing vacuum evaporation by using a rotary evaporator to remove ethyl alcohol and water, weighing, re-supplementing ultrapure water, heating in a water bath for dissolving, and filtering to obtain the sea buckthorn fruit water-soluble small molecules. Finally, filtering to obtain a sea buckthorn fruit water-soluble micromolecule extracting solution, and refrigerating and storing at 4 DEG C; according to the method, the water-soluble micromolecular chemical components of the sea buckthorn fruits are extracted by using a water extraction and high-concentration ethanol precipitation technology for the first time; the functional analysis shows that the water-soluble small molecule extract has antibacterial biological activity.

The invention discloses a novel application of beautiful millettia root polysaccharide as a synergist of an anti-tumor drug, and particularly relates to a combination of the beautiful millettia root polysaccharide and the anti-tumor drug paclitaxel, so that the anti-tumor effect of the paclitaxel can be improved. The invention further discloses a preparation method of the beautiful millettia root polysaccharide, and a detection method of the antitumor effect of the beautiful millettia root polysaccharide. The preparation method comprises the following steps: boiling a beautiful millettia root medicinal material, filtering, concentrating and centrifuging to obtain a first supernatant and a first precipitate; adding a proper amount of water into the first precipitate for dissolving, adjusting the pH to 2-4 by using hydrochloric acid, and centrifuging again to obtain a second supernatant; combining the first supernatant and the second supernatant, adding NaOH to adjust the pH value to 6-7, performing vacuum concentration, adding anhydrous 90% ethanol for precipitation, and performing suction filtration to obtain a second precipitate; washing the second precipitate with a solvent to obtain crude extract beautiful millettia root polysaccharide; finally, step-by-step elution is conducted through an ion exchange column, and purified beautiful millettia root polysaccharide is obtained after vacuum drying.

The invention belongs to the technical field of food and biological fermentation, and particularly relates to a rhodobryum tofu polysaccharide extract as well as a preparation method and application thereof. The preparation method of the rhodobryum tofu polysaccharide extract comprises the following steps: extracting rhodobryum tofu with hot water, filtering, taking filtrate, and concentrating to obtain a rhodobryum tofu aqueous extract; and precipitating the obtained aqueous extract with ethanol, centrifuging, taking the precipitate, dissolving the precipitate in ionized water, then removing protein by adopting a Sevag method to obtain a crude polysaccharide solution, filling the crude polysaccharide solution into a dialysis bag, dialyzing to remove small molecular substances, and freeze-drying to obtain the rhodotorula tofu polysaccharide extract. The polysaccharide functional components are extracted from the red fungus tofu as a raw material, and the polysaccharide content and component differences of the red fungus tofu obtained by different fermentation strains are verified. The application effect of the rhodobryum tofu polysaccharide extract in reducing the content of effective heavy metal Pb in food is verified, and the rhodobryum tofu polysaccharide extract has a good prospect in reducing the bioavailability of the heavy metal in the food, reducing the toxicity of the heavy metal and improving the food safety.

The invention discloses spirulina alpha-glucan containing soluble dietary fibers and having immunomodulatory activity and a preparation method thereof, spirulina is subjected to continuous illumination culture under nutrient salt deficiency or low temperature or combined regulation and control of low temperature and nutrient salt deficiency; a large amount of alpha-glucan which contains more soluble dietary fibers and has immunoregulatory activity is accumulated in the spirulina, and the spirulina alpha-glucan is prepared by adopting a method of hot water extraction, trichloroacetic acid deproteinization and ethanol precipitation. The prepared spirulina alpha-glucan contains 10%-26% of dietary fibers, the content of the dietary fibers is 9-27 times higher than the content of the dietary fibers of maltodextrin, the spirulina alpha-glucan can induce secretion of NO, TNF-alpha and IL-6 of macrophages RAW264.7, the activation index of the NO reaches 1.5-3.6, the activation index of the TNF-alpha reaches 2.0-4.1, the activation index of the IL-6 reaches 1.6-3.0, and the spirulina alpha-glucan has a remarkable immunoregulation effect. Technical support and product cases are provided for development of spirulina glucan type high-added-value functional products.

The present invention relates to the field of pharmaceuticals and health food technology, and specifically to a method for preparing and applying red date polysaccharides. The specific technical solution comprises the following steps: a method for preparing red date polysaccharides comprises concentrating jujubes with distilled water, removing proteins, and then precipitating with ethanol to obtain a primary ethanol precipitate containing polysaccharides; dialyzing the primary ethanol precipitate, and then precipitating with ethanol to obtain a secondary ethanol precipitate containing polysaccharides, which is then freeze-dried. The extracted red date polysaccharides can improve glucose utilization, increase GLUT4 protein expression, and prevent palmitate-induced insulin resistance and muscle damage in muscle cells, and can be used to prevent, improve, or treat anabolic or muscular diseases.

The application discloses a method for producing pectin oligosaccharides by microbial degradation of pomace and application thereof. The method comprises the following steps: adding microbacterium A5 to a culture medium containing pomace to perform fermentation to obtain a fermentation liquor, performing fractional pressing filtration and filter sterilization on the fermentation liquor, performing ethanol precipitation to extract a pectin crude product, removing protein, performing ion exchange chromatography, dialysis desalting, freeze drying and the like to purify two pectin oligosaccharides POS-1 and POS-2 with a purity greater than 95%; the pectin oligosaccharides POS-1 and POS-2 have strong scavenging capacity on hydroxyl radicals and DPPH free radicals and the capacity of inhibiting the activity of respiratory RNA viruses such as influenza virus H1N1 and respiratory syncytial virus RSV, and can be applied to medicines or health foods.

The invention relates to a method for simultaneously extracting dietary fiber and oligosaccharide from beet pulp, which comprises the following steps: mixing pretreated beet pulp with water, extracting, filtering to obtain a first filter residue and a first supernatant, mixing the first filter residue with alkali, and centrifuging to obtain a second filter residue and a second supernatant; mixing the second filter residue with an acid, and centrifuging to obtain a third filter residue and a third supernatant; mixing the third filter residue with an enzyme for enzymolysis, and centrifuging to obtain a fourth filter residue and a fourth supernate; mixing all the supernatants, performing ultrafiltration concentration to obtain a concentrated solution with the molecular weight of 500-10kDa, and performing freeze drying to obtain oligosaccharide; the concentrated solution with the molecular weight larger than 10 kDa is subjected to ethanol precipitation, and soluble dietary fibers are obtained; and mixing the fourth filter residue with a hydrogen peroxide solution, boiling, and filtering to obtain a precipitate which is the insoluble dietary fiber. According to the method, three substances are simultaneously extracted in one technological process, so that resources are comprehensively utilized, and the extraction efficiency is maximized.

The invention discloses a preparation method of long-chain oligonucleotide, and relates to the technical field of synthesis of long-chain oligonucleotide. According to the method, the desiliconization reaction is directly carried out after ammonolysis, ammonia removal is not needed, the quenching reaction is directly carried out after the desiliconization reaction, the steps of sample centrifugal concentration, drying, ethanol precipitation and the like are not needed, and the long-chain oligonucleotide is obtained through purification. By adopting a solid-phase synthesis method, the method has the technical advantages of high synthesis automation degree and short synthesis period on the whole, and the preparation process of the long-chain oligonucleotide is high in efficiency and fast in output. In addition, hydrophobic chromatography is arranged to tolerate a desiliconized crude product system, so that the loss of consumables is reduced, the nucleic acid synthesis cost is reduced, and large-scale synthesis of long-chain oligonucleotides is facilitated.

The invention belongs to the technical field of cactus polysaccharide, and discloses a method for extracting cactus polysaccharide by compound enzyme, which comprises the following steps: S1, pretreating fresh cactus to obtain cactus powder, sealing and storing; s2, adding a compound enzyme into the cactus powder, and carrying out polysaccharide extraction by utilizing ultrasonic waves; wherein the compound enzyme is any two or three of papain, cellulase and pectinase; s3, after ultrasonic extraction, enzyme deactivation and centrifugation are conducted, an upper-layer crude extracting solution is taken and concentrated, ethanol is added for precipitation, standing and centrifugation are conducted, precipitates continue to be washed with absolute ethyl alcohol, acetone and diethyl ether respectively, drying is conducted, and crude cactus polysaccharide is obtained. According to the method, the cactus polysaccharide is extracted by a compound enzyme enzyme method mainly comprising natural plant enzymes, so that the cost is reduced, the extraction purity is improved, and the cactus polysaccharide can be efficiently extracted.

The present invention provides a polysaccharide that has a protective effect on kidney damage. The polysaccharide of the present invention is obtained from the receptacle of Podocarpus carylus or Podocarpus brevifolia, plants of the Podocarpaceae family, after ultrasonic extraction, distilled water reheating extraction, reduced pressure concentration, ethanol precipitation, and freeze-drying. According to content determination, the mass percentage of the polysaccharide of the present invention is greater than or equal to 50% in terms of glucose. The polysaccharide is composed of 7 monosaccharides, including mannose, rhamnose, glucose, galactose, xylose, arabinose and L-fucose. In vivo experiments on mice have confirmed that the polysaccharide of the present invention has a good protective effect on kidney damage caused by cisplatin. The polysaccharide of the present invention can be used to prepare medicines with a protective effect on kidney damage.

The invention relates to saxifraga stolonifera polysaccharide and application thereof in preparation of complement inhibitors or anti-inflammatory drugs. Based on activity guidance, it is found that a water extraction part of saxifraga stolonifera has good anti-complement activity when experimental screening is conducted on the anti-complement active part of saxifraga stolonifera, graded alcohol precipitation is conducted on the water extraction part, the activity of crude polysaccharide precipitated by 90 alcohol (90% ethyl alcohol) is higher than that of crude polysaccharide precipitated by 75 alcohol (75% ethyl alcohol), and the anti-complement activity of the crude polysaccharide precipitated by 90 alcohol is higher than that of a positive drug (heparin). On the basis, five neutral heteropolysaccharides with anticomplement activity, namely SSP-90-1, SSP-90-2, SSP-90-3, SSP-90-4 or SSP-90-5, are separated from the 90 alcohol precipitation crude polysaccharide. Compared with the prior art, the structural characteristics of the saxifraga stolonifera polysaccharide are represented, and the preparation method of the saxifraga stolonifera polysaccharide and the application of the saxifraga stolonifera polysaccharide in preparation of complement inhibitors and anti-inflammatory drugs are provided. The invention verifies the anti-complement activity of the saxifraga stolonifera polysaccharide and the therapeutic effect of the saxifraga stolonifera polysaccharide on lipopolysaccharide (LPS)-induced acute lung injury and systemic inflammatory response.

The invention belongs to the technical field of natural medicine extraction and application, and particularly relates to endive polysaccharide extract and an extraction method and application thereof.The endive polysaccharide extract is obtained by extracting and refining perennial herbaceous plant endive stems and leaves, and specifically comprises the steps that crude polysaccharide is extracted through a water boiling and alcohol precipitation method, and the crude polysaccharide is extracted through an ethanol precipitation method; the preparation method comprises the following steps: removing impurity proteins contained in a coarsely extracted extract through a sevage method, removing polysaccharide components with relatively low content from the obtained deproteinized polysaccharide through DEAE-52 cellulose chromatography, retaining main part polysaccharide with high content, and precipitating and refining to obtain the deproteinized polysaccharide. The endive polysaccharide extract is a main part of endive polysaccharide, and is a second elution peak part which is eluted by a DEAE-52 cellulose chromatographic column and has the highest content. The endive polysaccharide prepared by the invention can be used for treating ulcerative colitis.

The invention discloses an ethanol precipitation and separation device based on a konjac glucomannan preparation process, and relates to the field of konjac glucomannan preparation, the ethanol precipitation and separation device comprises a mounting rack, and a liquid accumulation barrel and a material mixing barrel which are fixedly assembled on the mounting rack, the material mixing barrel is located above the liquid accumulation barrel, and the liquid accumulation barrel and the material mixing barrel are concentrically distributed; the top of the material mixing barrel is in a closed state, an arc-shaped door capable of being opened and closed is arranged on the side wall of the liquid accumulation barrel, and a detachable closing cover is embedded in the top of the material mixing barrel. Under the action of the centrifugal separation unit, liquid in the mixed solution is discharged, sediments are left in the centrifugal separation unit, and then under the action of the sediment collection unit, the sediments in the centrifugal separation unit are automatically collected and treated, so that workers do not need to take out the sediments in the centrifugal separation unit.

The invention belongs to the technical field of plant active ingredient extraction, and relates to a segmented enzymolysis-cascade membrane separation extraction method of cyclocarya paliurus combined-state selenium polysaccharide and the combined-state selenium polysaccharide prepared by the method. The method comprises the following steps: firstly, constructing a first extraction system for a cyclocarya paliurus leaf raw material, carrying out first-stage enzymolysis, inactivation and solid-liquid separation, separating a first liquid phase through a nanofiltration membrane to form a low-molecular selenium-containing component flow, and excluding the low-molecular selenium-containing component flow in a subsequent alcohol precipitation and recovery step; and constructing a second extraction system from the first solid phase, carrying out second-stage enzymolysis, inactivation and solid-liquid separation, sequentially carrying out microfiltration, first ultrafiltration, second ultrafiltration and nanofiltration washing on the second liquid phase, and carrying out ethanol precipitation to obtain the combined-state selenium polysaccharide. The proportion of target molecular weight interval components in the obtained product is increased, the mass fraction of polysaccharide is increased, and the proportion of inorganic selenium, the mass fraction of protein and the ash content are reduced.

The invention relates to a preparation method and application of novel rhizoma bletillae glucomannan. The preparation method of the rhizoma bletillae glucomannan comprises the following steps: 1, drying, crushing and sieving rhizomes of a raw material rhizoma bletillae; 2, putting the crushed and sieved rhizoma bletillae into ethanol for degreasing; 3, obtaining crude polysaccharide through a distilled water ice bath ultrasonic extraction technology and an ethanol precipitation technology; 4, separating and purifying through a DEAE-52 cellulose column and a G-150 gel column to obtain the high-purity rhizoma bletillae homogeneous polysaccharide BsPsIII1-1, namely the rhizoma bletillae glucomannan; 5, carrying out semipermeable membrane dialysis on the BsPsIII1-1 to remove salt, and carrying out freeze drying; and 6, carrying out chemical analysis on the obtained BsPsIII1-1. According to the preparation method and the application of the novel rhizoma bletillae glucomannan, animal experiments show that BsPsIII1-1 can significantly improve CCl4 induced mouse liver tissue injury, reduce serum ALT and AST levels, effectively relieve oxidative stress by reducing MDA content and increasing GSH level, and significantly inhibit hepatic fibrosis by down-regulating expression of alpha smooth muscle actin (alpha-SMA) and collagen I (COL-1).

Embodiments of the invention include a method of purifying circular RNA (circRNA) from a solution. The method an include steps of (a) incubating the solution at about 55°C, (b) cellulose chromatography, (c) desalting and purifying RNA reactions with magnetic carboxylic acid beads, (d) ethanol precipitation to remove double stranded RNA byproducts and concentrated RNA, (e) poly-A polymerase treatment, (f) desalting and purifying RNA reactions with magnetic carboxylic acid beads to remove poly A reaction components, (g) RNase R treatment, and (h) desalting and purifying the solution to yield purified circRNA. In aspects, the method removes, among other contaminates, in vitro transcribed circular RNA from linear and doubled stranded RNA.

This invention discloses the application of Uncaria rhynchophylla neutral polysaccharide URP-W in the preparation of anti-glioma drugs, belonging to the field of anti-tumor technology of medicinal plant polysaccharides. The invention obtains a crude polysaccharide extract from Uncaria rhynchophylla through hot water extraction and ethanol precipitation, followed by purification using a DEAE cellulose column and G200 dextran gel to obtain Uncaria rhynchophylla neutral polysaccharide URP-W. The monosaccharide composition of URP-W includes arabinose, rhamnose, galactose, glucose, xylose, mannose, galacturonic acid, and mannuronic acid in a molar ratio of 17.99:6.32:21.71:27.17:2.56:7.57:16.20:0.49. Experiments have shown that URP-W can inhibit the proliferation of gliomas and induce apoptosis, exhibiting significant anti-tumor activity against glioma cells, providing a new drug raw material and treatment method for the clinical treatment of gliomas.

The invention discloses a method for extracting polysaccharide from pomace of sorbus pomace, and belongs to the technical field of separation of active ingredients of natural products. The method comprises the following steps: drying, crushing and sieving sorbus pomace, putting the sorbus pomace into an extraction kettle, pumping water into the extraction kettle, carrying out subcritical extraction for 10-50 minutes, cooling, carrying out solid-liquid separation, deproteinizing with trichloroacetic acid, centrifuging, precipitating supernate with ethanol, collecting the precipitate, and freeze-drying to obtain the sorbus pomace polysaccharide. The extraction yield of the sorbus pomace polysaccharide is high, the energy consumption is low, the process is clean and environment-friendly, the process is simple, and the structure and function research of the sorbus pomace polysaccharide and the development of high-added-value products of the sorbus pomace polysaccharide are more convenient.

The invention discloses a heparin sodium purification method and application thereof, and the purification method comprises the following steps: S1, enzymolysis pretreatment: adding an animal mucous membrane liquid into a reaction kettle, adjusting the pH value, and carrying out enzymolysis at the temperature; s2, ultrasonic-assisted treatment: applying ultrasonic waves to the enzymatic hydrolysate; s3, adding a protective agent, namely adding a monosaccharide or disaccharide protective agent; s4, purification with ion exchange resin: dynamically adsorbing the heparin sodium by using strongly basic anion exchange resin, and eluting; s5, ethanol precipitation and ultrafiltration: ethanol is added, heparin sodium is precipitated, and then micromolecular impurities are removed through ultrafiltration; and S6, freeze-drying and forming: freeze-drying to obtain a finished product of heparin sodium. The invention relates to the technical field of biological medicine. According to the purification method and application of the heparin sodium, efficient separation and purification of the heparin sodium are achieved through the synergistic effect of multiple steps of enzymolysis, ultrasonic-assisted treatment, protective agent stabilization, ion exchange resin purification, ethanol precipitation and the like, the titer and purity of the product are remarkably improved, and the purification method is suitable for industrial production.

This invention discloses a method for extracting high-purity PDRN from salmon testes and its application in improving skin condition, belonging to the field of bioactive ingredient extraction technology. Frozen salmon testis tissue is cleaned of blood vessels and fatty impurities, then cryogenically pulverized at -20~-25℃. The pulverized tissue is added to a pre-treatment dispersion buffer and homogenized under high pressure to obtain a DNA fragmentation solution. The precipitate is then separated by centrifugation. The precipitate is dispersed in a dedicated enzyme digestion buffer, and enzymatic digestion with protease and RNase A is performed to inactivate the enzyme. DNase I is added to the digestion solution for enzymatic cleavage. After the reaction, a stop solution is added to terminate the reaction, and the supernatant is separated by centrifugation. Ethanol is added to the supernatant for precipitation, and centrifugation yields PDRN from salmon testes. This invention, through specific buffer systems and enzymatic digestion conditions, increases the PDRN content by 40~60 kDa, allowing for better absorption and utilization by the skin, improving facial hydration and elasticity, effectively reducing facial wrinkles, and improving facial skin condition. It can be used to prepare functional cosmetics.

The invention belongs to the technical field of natural product extraction, and particularly relates to extraction and derivatization of water-soluble apple polysaccharide and application of the water-soluble apple polysaccharide in cigarettes. The extraction method of the water-soluble apple polysaccharide comprises the following steps: freezing and centrifuging apple pulp, filtering to obtain a solution A, precipitating with 95% ethanol, freezing and centrifuging overnight, filtering to obtain an alcohol-insoluble substance B, drying under reduced pressure until no alcohol smell exists, dispersing in a proper amount of water to obtain a solution B, concentrating to a proper amount, performing deproteinization treatment, stirring, standing, centrifuging, and separating out supernate, thereby obtaining the water-soluble apple polysaccharide. And carrying out column chromatography treatment to obtain a solution D, carrying out membrane separation treatment by using filter membranes with different pore diameters, concentrating, and freeze-drying to obtain the refined apple polysaccharide. In a solution state, the apple polysaccharide can form a stable system with certain viscosity. Meanwhile, the apple polysaccharide also has certain oxidation resistance, and can delay the oxidation process of the system to a certain extent and maintain the stability of the components.

The invention relates to a purification and application method of a self-assembled supramolecular substance based on a radix puerariae, scutellaria baicalensis, coptis chinensis and honey-fried licorice root decoction, which comprises the following steps: proportioning radix puerariae, scutellaria baicalensis, coptis chinensis and honey-fried licorice root, adding water, decocting to obtain a GQD decoction, sequentially carrying out ultrafiltration and alcohol precipitation treatment on the supernatant of the GQD decoction, volatilizing the obtained supernatant, redissolving, and freeze-drying to obtain the self-assembled supramolecular substance, and purifying to obtain the self-assembled supramolecular substance based on the self-assembled supramolecular substance based on the radix puerariae, scutellaria baicalensis, coptis chinensis and honey-fried licorice root decoction. The compound is further used for preparing anti-inflammatory and anti-UC drugs; according to the invention, the anti-UC characteristic of the traditional Chinese medicine composition is utilized to recover intestinal flora with UC disorder and repair damaged intestinal barriers, meanwhile, core pharmacodynamic substances are reserved to the greatest extent, other irrelevant or harmful impurities can be reduced, and the traditional Chinese medicine composition is suitable for separating and purifying a nano-scale supramolecular compound with biological activity in a traditional Chinese medicine decoction to improve the curative effect and reduce toxic and side effects, so that the traditional Chinese medicine composition is suitable for clinical application. And the intestinal micro-ecology and mucous membrane barrier function can be repaired.