224 results about "Ethanol precipitation" patented technology

The invention relates to the technical field of plant polysaccharide extraction, in particular to a process for synergistically extracting rape pollen polysaccharide by a compound enzyme, which comprises the following steps: crushing rape pollen to 200-300 meshes by pulse airflow, performing microwave-ultrasonic degreasing by using a 85% ethanol solution, mixing with an acetic acid-sodium acetate buffer solution with the pH value of 4.8, and performing ultrasonic extraction to obtain a crude extract; the method comprises the following steps: adding a compound enzyme system which is immobilized by Fe3O4 (at) SiO2 nanoparticles by cellulase, pectinase, protease and beta-glucosidase according to a mass ratio of 2: 1: 1: 0.5, carrying out enzymolysis for 4 hours in a 50 DEG C constant-temperature water bath and a 15V / cm pulsed electric field, carrying out enzyme deactivation on enzymatic hydrolysate, carrying out gradient ethanol precipitation, carrying out ultrafiltration deproteinization, and carrying out vacuum pulsation freeze drying to obtain the polysaccharide. According to the method disclosed by the invention, the cell wall breakage rate and the enzymolysis efficiency are improved through pulsed airflow crushing, magnetic nano immobilized enzyme and pulsed electric field assistance, the polysaccharide yield reaches 7.32%, the purity is 91%, the beta-glucosidic bond proportion and the antioxidant activity are remarkably improved, the enzyme can be recycled and reused, the drying time is shortened, and efficient and low-consumption extraction is realized.

The invention discloses a method for improving the conversion efficiency of insoluble dietary fibers and soluble dietary fibers obtained by the method, and belongs to the technical field of food processing and functional ingredient modification. The method comprises the following steps: performing superfine grinding treatment on wheat bran to obtain wheat bran powder; adding water into wheat bran powder, and then carrying out high-temperature and high-pressure treatment to obtain a wheat bran solution; then carrying out stepped enzymolysis on the wheat bran solution to obtain enzymatic hydrolysate; the enzymatic hydrolysate is centrifuged, lower-layer solid precipitates are insoluble dietary fibers, supernate is subjected to ethanol precipitation, and the soluble dietary fibers are obtained. The wheat bran is modified by combining superfine grinding and high-temperature and high-pressure pretreatment with stepped enzymolysis, so that the yield of the soluble dietary fibers is remarkably increased, and the functional characteristics of the soluble dietary fibers are improved. The obtained soluble dietary fiber has excellent physicochemical properties and physiological functions, and can be widely applied to baked foods, beverages, dietary supplements and other products, so that the soluble dietary fiber has important application prospects and value in the technical field of food processing.

The invention discloses application of uncaria acidic polysaccharide URP-2 in preparation of an anti-esophageal cancer drug, and belongs to the technical field of antitumor of medicinal plant polysaccharide. According to the method, a method for extracting the uncaria total polysaccharide by boiling water and precipitating the uncaria total polysaccharide by ethanol is adopted, and a DEAE DE-52 cellulose column and sephadex column purification technology is combined, so that the separation of the uncaria acidic polysaccharide component is realized, and the uncaria acidic polysaccharide URP-2 is obtained. The uncaria acidic polysaccharide URP-2 comprises the following components in mole percent: 14.11% of arabinose, 12.64% of rhamnose, 6.48% of galactose, 5.00% of glucose and 61.77% of galacturonic acid. Experiments show that the uncaria acidic polysaccharide URP-2 has remarkable anti-esophageal cancer activity, so that a potential experimental basis and a new strategy are provided for applying the uncaria acidic polysaccharide URP-2 to clinical treatment of esophageal cancer.

The invention discloses a digestion-aiding highland barley fried malt polysaccharide as well as a preparation method and application thereof. The polysaccharide is extracted from highland barley through hot water extraction, ethanol precipitation, deproteinization, ion exchange column chromatography, gel column chromatography and dialysis processes. Animal experiments show that the polysaccharide can significantly improve the small intestine propulsion rate of mice and increase the weight increment, gastric juice amount, pepsin activity and pepsin discharge of rats, and the effect of the polysaccharide is superior to that of traditional roasted barley malt polysaccharide; the highland barley polysaccharide is superior to highland barley crude polysaccharide, highland barley polyphenol, highland barley lipid, highland barley protein and the like. The invention has the advantages of easily available raw materials and green process, is suitable for developing side-effect-free digestion-aiding functional foods such as tea bags, milk tea, yoghurt and biscuits, and has important market value.

The invention relates to the technical field of plant polysaccharides, and particularly discloses a red-knees herb polysaccharide and a preparation method and application thereof, the peak top molecular weight of the red-knees herb polysaccharide is 33.9 KDa, the weight-average molecular weight of the red-knees herb polysaccharide is 42.8 KDa, the number-average molecular weight of the red-knees herb polysaccharide is 10.0 KDa, and the red-knees herb polysaccharide is mainly composed of galacturonic acid, galactose, arabinose, rhamnose and glucuronic acid. According to the preparation method of the red-knees herb polysaccharide, by optimizing process parameters and controlling the reasonable solid-liquid ratio, heating temperature and heating time, the dissolution rate of the polysaccharide is increased, the dissolution rate of impurities is reduced, and the yield of the red-knees herb polysaccharide is increased. The red-knees herb polysaccharide is used as a raw material, ethanol is used for alcohol precipitation, polysaccharide components are precipitated, impurities are preliminarily removed, then macroporous resin is used for purification, the recovery rate of polysaccharide and the removal rate of impurities such as pigment and protein are improved by screening optimization and pretreatment of the macroporous resin, and the total sugar content of the purified red-knees herb polysaccharide is 80% or above. Use of toxic chemical reagents is avoided, and the preparation method is safe and efficient.

The invention discloses a degradable nanocellulose sustained-release drug-loaded film and a preparation method thereof, and belongs to the technical field of drug functional materials. The preparation method comprises the following steps: mixing and stirring microcrystalline cellulose and 64wt% sulfuric acid solution, centrifuging, dialyzing to be neutral, and freeze-drying to obtain CNCs; cNCs, NaIO4 and water are mixed and then subjected to a light-shielding reaction, ethanol precipitation is added, dialysis purification is conducted, freeze drying is conducted, and formylation modified CNCs are obtained; mixing and stirring the aldehyde modified CNCs, sericin, polyvinyl alcohol, water and a target drug to obtain a core layer solution; dissolving a polylactic acid-glycolic acid copolymer into a mixed solution of chloroform and DMF (Dimethyl Formamide) to obtain a shell solution; the core layer solution and the shell layer solution are subjected to coaxial electrostatic spinning forming to obtain the drug-loaded fiber, then the drug-loaded fiber is subjected to biomimetic mineralization surface modification to obtain the degradable nanocellulose sustained-release drug-loaded film, the drug loading rate is increased, the mechanical property of the drug-loaded film is improved, and the sustained-release and release-controllable effects are achieved.

The invention discloses a novel application of beautiful millettia root polysaccharide as a synergist of an anti-tumor drug, and particularly relates to a combination of the beautiful millettia root polysaccharide and the anti-tumor drug paclitaxel, so that the anti-tumor effect of the paclitaxel can be improved. The invention further discloses a preparation method of the beautiful millettia root polysaccharide, and a detection method of the antitumor effect of the beautiful millettia root polysaccharide. The preparation method comprises the following steps: boiling a beautiful millettia root medicinal material, filtering, concentrating and centrifuging to obtain a first supernatant and a first precipitate; adding a proper amount of water into the first precipitate for dissolving, adjusting the pH to 2-4 by using hydrochloric acid, and centrifuging again to obtain a second supernatant; combining the first supernatant and the second supernatant, adding NaOH to adjust the pH value to 6-7, performing vacuum concentration, adding anhydrous 90% ethanol for precipitation, and performing suction filtration to obtain a second precipitate; washing the second precipitate with a solvent to obtain crude extract beautiful millettia root polysaccharide; finally, step-by-step elution is conducted through an ion exchange column, and purified beautiful millettia root polysaccharide is obtained after vacuum drying.

The invention belongs to the technical field of food and biological fermentation, and particularly relates to a rhodobryum tofu polysaccharide extract as well as a preparation method and application thereof. The preparation method of the rhodobryum tofu polysaccharide extract comprises the following steps: extracting rhodobryum tofu with hot water, filtering, taking filtrate, and concentrating to obtain a rhodobryum tofu aqueous extract; and precipitating the obtained aqueous extract with ethanol, centrifuging, taking the precipitate, dissolving the precipitate in ionized water, then removing protein by adopting a Sevag method to obtain a crude polysaccharide solution, filling the crude polysaccharide solution into a dialysis bag, dialyzing to remove small molecular substances, and freeze-drying to obtain the rhodotorula tofu polysaccharide extract. The polysaccharide functional components are extracted from the red fungus tofu as a raw material, and the polysaccharide content and component differences of the red fungus tofu obtained by different fermentation strains are verified. The application effect of the rhodobryum tofu polysaccharide extract in reducing the content of effective heavy metal Pb in food is verified, and the rhodobryum tofu polysaccharide extract has a good prospect in reducing the bioavailability of the heavy metal in the food, reducing the toxicity of the heavy metal and improving the food safety.

The present invention relates to the field of pharmaceuticals and health food technology, and specifically to a method for preparing and applying red date polysaccharides. The specific technical solution comprises the following steps: a method for preparing red date polysaccharides comprises concentrating jujubes with distilled water, removing proteins, and then precipitating with ethanol to obtain a primary ethanol precipitate containing polysaccharides; dialyzing the primary ethanol precipitate, and then precipitating with ethanol to obtain a secondary ethanol precipitate containing polysaccharides, which is then freeze-dried. The extracted red date polysaccharides can improve glucose utilization, increase GLUT4 protein expression, and prevent palmitate-induced insulin resistance and muscle damage in muscle cells, and can be used to prevent, improve, or treat anabolic or muscular diseases.

The application discloses a method for producing pectin oligosaccharides by microbial degradation of pomace and application thereof. The method comprises the following steps: adding microbacterium A5 to a culture medium containing pomace to perform fermentation to obtain a fermentation liquor, performing fractional pressing filtration and filter sterilization on the fermentation liquor, performing ethanol precipitation to extract a pectin crude product, removing protein, performing ion exchange chromatography, dialysis desalting, freeze drying and the like to purify two pectin oligosaccharides POS-1 and POS-2 with a purity greater than 95%; the pectin oligosaccharides POS-1 and POS-2 have strong scavenging capacity on hydroxyl radicals and DPPH free radicals and the capacity of inhibiting the activity of respiratory RNA viruses such as influenza virus H1N1 and respiratory syncytial virus RSV, and can be applied to medicines or health foods.

The invention belongs to the technical field of cactus polysaccharide, and discloses a method for extracting cactus polysaccharide by compound enzyme, which comprises the following steps: S1, pretreating fresh cactus to obtain cactus powder, sealing and storing; s2, adding a compound enzyme into the cactus powder, and carrying out polysaccharide extraction by utilizing ultrasonic waves; wherein the compound enzyme is any two or three of papain, cellulase and pectinase; s3, after ultrasonic extraction, enzyme deactivation and centrifugation are conducted, an upper-layer crude extracting solution is taken and concentrated, ethanol is added for precipitation, standing and centrifugation are conducted, precipitates continue to be washed with absolute ethyl alcohol, acetone and diethyl ether respectively, drying is conducted, and crude cactus polysaccharide is obtained. According to the method, the cactus polysaccharide is extracted by a compound enzyme enzyme method mainly comprising natural plant enzymes, so that the cost is reduced, the extraction purity is improved, and the cactus polysaccharide can be efficiently extracted.

The present invention provides a polysaccharide that has a protective effect on kidney damage. The polysaccharide of the present invention is obtained from the receptacle of Podocarpus carylus or Podocarpus brevifolia, plants of the Podocarpaceae family, after ultrasonic extraction, distilled water reheating extraction, reduced pressure concentration, ethanol precipitation, and freeze-drying. According to content determination, the mass percentage of the polysaccharide of the present invention is greater than or equal to 50% in terms of glucose. The polysaccharide is composed of 7 monosaccharides, including mannose, rhamnose, glucose, galactose, xylose, arabinose and L-fucose. In vivo experiments on mice have confirmed that the polysaccharide of the present invention has a good protective effect on kidney damage caused by cisplatin. The polysaccharide of the present invention can be used to prepare medicines with a protective effect on kidney damage.

The invention relates to saxifraga stolonifera polysaccharide and application thereof in preparation of complement inhibitors or anti-inflammatory drugs. Based on activity guidance, it is found that a water extraction part of saxifraga stolonifera has good anti-complement activity when experimental screening is conducted on the anti-complement active part of saxifraga stolonifera, graded alcohol precipitation is conducted on the water extraction part, the activity of crude polysaccharide precipitated by 90 alcohol (90% ethyl alcohol) is higher than that of crude polysaccharide precipitated by 75 alcohol (75% ethyl alcohol), and the anti-complement activity of the crude polysaccharide precipitated by 90 alcohol is higher than that of a positive drug (heparin). On the basis, five neutral heteropolysaccharides with anticomplement activity, namely SSP-90-1, SSP-90-2, SSP-90-3, SSP-90-4 or SSP-90-5, are separated from the 90 alcohol precipitation crude polysaccharide. Compared with the prior art, the structural characteristics of the saxifraga stolonifera polysaccharide are represented, and the preparation method of the saxifraga stolonifera polysaccharide and the application of the saxifraga stolonifera polysaccharide in preparation of complement inhibitors and anti-inflammatory drugs are provided. The invention verifies the anti-complement activity of the saxifraga stolonifera polysaccharide and the therapeutic effect of the saxifraga stolonifera polysaccharide on lipopolysaccharide (LPS)-induced acute lung injury and systemic inflammatory response.

The invention belongs to the technical field of plant active ingredient extraction, and relates to a segmented enzymolysis-cascade membrane separation extraction method of cyclocarya paliurus combined-state selenium polysaccharide and the combined-state selenium polysaccharide prepared by the method. The method comprises the following steps: firstly, constructing a first extraction system for a cyclocarya paliurus leaf raw material, carrying out first-stage enzymolysis, inactivation and solid-liquid separation, separating a first liquid phase through a nanofiltration membrane to form a low-molecular selenium-containing component flow, and excluding the low-molecular selenium-containing component flow in a subsequent alcohol precipitation and recovery step; and constructing a second extraction system from the first solid phase, carrying out second-stage enzymolysis, inactivation and solid-liquid separation, sequentially carrying out microfiltration, first ultrafiltration, second ultrafiltration and nanofiltration washing on the second liquid phase, and carrying out ethanol precipitation to obtain the combined-state selenium polysaccharide. The proportion of target molecular weight interval components in the obtained product is increased, the mass fraction of polysaccharide is increased, and the proportion of inorganic selenium, the mass fraction of protein and the ash content are reduced.

The invention relates to a preparation method and application of novel rhizoma bletillae glucomannan. The preparation method of the rhizoma bletillae glucomannan comprises the following steps: 1, drying, crushing and sieving rhizomes of a raw material rhizoma bletillae; 2, putting the crushed and sieved rhizoma bletillae into ethanol for degreasing; 3, obtaining crude polysaccharide through a distilled water ice bath ultrasonic extraction technology and an ethanol precipitation technology; 4, separating and purifying through a DEAE-52 cellulose column and a G-150 gel column to obtain the high-purity rhizoma bletillae homogeneous polysaccharide BsPsIII1-1, namely the rhizoma bletillae glucomannan; 5, carrying out semipermeable membrane dialysis on the BsPsIII1-1 to remove salt, and carrying out freeze drying; and 6, carrying out chemical analysis on the obtained BsPsIII1-1. According to the preparation method and the application of the novel rhizoma bletillae glucomannan, animal experiments show that BsPsIII1-1 can significantly improve CCl4 induced mouse liver tissue injury, reduce serum ALT and AST levels, effectively relieve oxidative stress by reducing MDA content and increasing GSH level, and significantly inhibit hepatic fibrosis by down-regulating expression of alpha smooth muscle actin (alpha-SMA) and collagen I (COL-1).

Embodiments of the invention include a method of purifying circular RNA (circRNA) from a solution. The method an include steps of (a) incubating the solution at about 55°C, (b) cellulose chromatography, (c) desalting and purifying RNA reactions with magnetic carboxylic acid beads, (d) ethanol precipitation to remove double stranded RNA byproducts and concentrated RNA, (e) poly-A polymerase treatment, (f) desalting and purifying RNA reactions with magnetic carboxylic acid beads to remove poly A reaction components, (g) RNase R treatment, and (h) desalting and purifying the solution to yield purified circRNA. In aspects, the method removes, among other contaminates, in vitro transcribed circular RNA from linear and doubled stranded RNA.

This invention discloses the application of Uncaria rhynchophylla neutral polysaccharide URP-W in the preparation of anti-glioma drugs, belonging to the field of anti-tumor technology of medicinal plant polysaccharides. The invention obtains a crude polysaccharide extract from Uncaria rhynchophylla through hot water extraction and ethanol precipitation, followed by purification using a DEAE cellulose column and G200 dextran gel to obtain Uncaria rhynchophylla neutral polysaccharide URP-W. The monosaccharide composition of URP-W includes arabinose, rhamnose, galactose, glucose, xylose, mannose, galacturonic acid, and mannuronic acid in a molar ratio of 17.99:6.32:21.71:27.17:2.56:7.57:16.20:0.49. Experiments have shown that URP-W can inhibit the proliferation of gliomas and induce apoptosis, exhibiting significant anti-tumor activity against glioma cells, providing a new drug raw material and treatment method for the clinical treatment of gliomas.

The invention discloses a heparin sodium purification method and application thereof, and the purification method comprises the following steps: S1, enzymolysis pretreatment: adding an animal mucous membrane liquid into a reaction kettle, adjusting the pH value, and carrying out enzymolysis at the temperature; s2, ultrasonic-assisted treatment: applying ultrasonic waves to the enzymatic hydrolysate; s3, adding a protective agent, namely adding a monosaccharide or disaccharide protective agent; s4, purification with ion exchange resin: dynamically adsorbing the heparin sodium by using strongly basic anion exchange resin, and eluting; s5, ethanol precipitation and ultrafiltration: ethanol is added, heparin sodium is precipitated, and then micromolecular impurities are removed through ultrafiltration; and S6, freeze-drying and forming: freeze-drying to obtain a finished product of heparin sodium. The invention relates to the technical field of biological medicine. According to the purification method and application of the heparin sodium, efficient separation and purification of the heparin sodium are achieved through the synergistic effect of multiple steps of enzymolysis, ultrasonic-assisted treatment, protective agent stabilization, ion exchange resin purification, ethanol precipitation and the like, the titer and purity of the product are remarkably improved, and the purification method is suitable for industrial production.

This invention discloses a method for extracting high-purity PDRN from salmon testes and its application in improving skin condition, belonging to the field of bioactive ingredient extraction technology. Frozen salmon testis tissue is cleaned of blood vessels and fatty impurities, then cryogenically pulverized at -20~-25℃. The pulverized tissue is added to a pre-treatment dispersion buffer and homogenized under high pressure to obtain a DNA fragmentation solution. The precipitate is then separated by centrifugation. The precipitate is dispersed in a dedicated enzyme digestion buffer, and enzymatic digestion with protease and RNase A is performed to inactivate the enzyme. DNase I is added to the digestion solution for enzymatic cleavage. After the reaction, a stop solution is added to terminate the reaction, and the supernatant is separated by centrifugation. Ethanol is added to the supernatant for precipitation, and centrifugation yields PDRN from salmon testes. This invention, through specific buffer systems and enzymatic digestion conditions, increases the PDRN content by 40~60 kDa, allowing for better absorption and utilization by the skin, improving facial hydration and elasticity, effectively reducing facial wrinkles, and improving facial skin condition. It can be used to prepare functional cosmetics.

The invention belongs to the technical field of natural product extraction, and particularly relates to extraction and derivatization of water-soluble apple polysaccharide and application of the water-soluble apple polysaccharide in cigarettes. The extraction method of the water-soluble apple polysaccharide comprises the following steps: freezing and centrifuging apple pulp, filtering to obtain a solution A, precipitating with 95% ethanol, freezing and centrifuging overnight, filtering to obtain an alcohol-insoluble substance B, drying under reduced pressure until no alcohol smell exists, dispersing in a proper amount of water to obtain a solution B, concentrating to a proper amount, performing deproteinization treatment, stirring, standing, centrifuging, and separating out supernate, thereby obtaining the water-soluble apple polysaccharide. And carrying out column chromatography treatment to obtain a solution D, carrying out membrane separation treatment by using filter membranes with different pore diameters, concentrating, and freeze-drying to obtain the refined apple polysaccharide. In a solution state, the apple polysaccharide can form a stable system with certain viscosity. Meanwhile, the apple polysaccharide also has certain oxidation resistance, and can delay the oxidation process of the system to a certain extent and maintain the stability of the components.

The invention relates to a purification and application method of a self-assembled supramolecular substance based on a radix puerariae, scutellaria baicalensis, coptis chinensis and honey-fried licorice root decoction, which comprises the following steps: proportioning radix puerariae, scutellaria baicalensis, coptis chinensis and honey-fried licorice root, adding water, decocting to obtain a GQD decoction, sequentially carrying out ultrafiltration and alcohol precipitation treatment on the supernatant of the GQD decoction, volatilizing the obtained supernatant, redissolving, and freeze-drying to obtain the self-assembled supramolecular substance, and purifying to obtain the self-assembled supramolecular substance based on the self-assembled supramolecular substance based on the radix puerariae, scutellaria baicalensis, coptis chinensis and honey-fried licorice root decoction. The compound is further used for preparing anti-inflammatory and anti-UC drugs; according to the invention, the anti-UC characteristic of the traditional Chinese medicine composition is utilized to recover intestinal flora with UC disorder and repair damaged intestinal barriers, meanwhile, core pharmacodynamic substances are reserved to the greatest extent, other irrelevant or harmful impurities can be reduced, and the traditional Chinese medicine composition is suitable for separating and purifying a nano-scale supramolecular compound with biological activity in a traditional Chinese medicine decoction to improve the curative effect and reduce toxic and side effects, so that the traditional Chinese medicine composition is suitable for clinical application. And the intestinal micro-ecology and mucous membrane barrier function can be repaired.

The invention discloses a poria cocos water-soluble polysaccharide preparation method based on enzyme-alkali synergy. The poria cocos water-soluble polysaccharide preparation method comprises the following steps: S1, crushing poria cocos to obtain poria cocos powder; mixing with ethanol, stirring and extracting; mixing with distilled water, stirring and extracting, and finally drying; s2, mixing the obtained system with a sodium hydroxide solution, stirring at room temperature, and centrifuging to obtain supernate; adjusting the pH value of the supernate by using an acetic acid solution, standing, repeatedly washing by using distilled water, and freeze-drying to obtain the alkali-soluble polysaccharide of the poria cocos; and S3, dissolving the obtained system in a sodium hydroxide solution, adding a compound enzyme, carrying out an enzymatic hydrolysis reaction, and carrying out enzyme deactivation, centrifugation, tetraploid volume ethanol precipitation, dialysis and freeze-drying on an enzymatic hydrolysate to obtain the poria cocos water-soluble polysaccharide. The invention provides a poria cocos water-soluble polysaccharide preparation method based on enzyme-alkali synergy, poria cocos alkali-soluble polysaccharide is used as a substrate, the poria cocos water-soluble polysaccharide is prepared by complex enzyme, and the extraction rate and activity of the poria cocos water-soluble polysaccharide are improved. The invention further discloses application of the poria cocos water-soluble polysaccharide.

The invention relates to the field of oral liquid, provides functional oral liquid containing dandelion extract and a preparation method of the functional oral liquid, and solves the problems that an existing product is unstable in component and bitter and astringent in taste. The method comprises the following steps: carrying out enzymolysis extraction, ultrasonic-assisted purification, ethanol precipitation and freeze drying on a dandelion extract to obtain purified polysaccharide, compounding carboxymethyl chitosan modified sodium alginate, pectin and whey protein, preparing a W / O type emulsion by combining calcium carbonate nanoparticles, and carrying out gelation and freeze drying to form the microcapsule. And finally, adding into an oral liquid matrix to prepare a product with good taste and high stability. The particle size of the obtained microcapsule is 8-12 [mu] m, the encapsulation efficiency is greater than or equal to 90%, the bitter taste can be effectively masked, the bitter taste value is reduced to 8.0-11.0 BU, the storage stability reaches 150-180 days, and the microcapsule has a relatively high application value.

The invention discloses lychee chitosan as well as an extraction method and application thereof. The lychee shell polysaccharide is successfully separated and purified from lychee shells through a series of processes such as hot water ultrasonic extraction, ethanol precipitation, deproteinization, ion exchange column chromatography and gel column chromatography. The lychee chitosan is heteropolysaccharide and comprises arabinose, galactose, glucose and galacturonic acid, and the molar mass ratio of the arabinose to the galactose to the glucose to the galacturonic acid is (27-29): (13-15): (13-15): (18-19). The lychee shell polysaccharide prepared by the method has good oil holding capacity and hygroscopicity, and due to the characteristics, the lychee shell polysaccharide can be used as a preparation auxiliary material and is widely applied to the fields of medicines, foods, health care products and cosmetics. In addition, the polysaccharide has a remarkable inhibition effect on alpha-amylase and alpha-glucosidase, and due to the characteristic, the polysaccharide has an extremely wide application prospect in the aspect of developing hypoglycemic drugs, functional foods and health-care products.

The invention discloses application of uncaria acidic polysaccharide URP-1 in preparation of anti-prostatic cancer drugs, and belongs to the technical field of antitumor of medicinal plant polysaccharides. The preparation method comprises the following steps: obtaining uncaria total polysaccharide from uncaria through boiling water extraction and ethanol precipitation methods, and then purifying the total polysaccharide through DEAE cellulose and sephadex G200 to obtain the uncaria acidic polysaccharide URP-1. The URP-1 comprises the following components in mole percent: 28.68% of arabinose, 11.08% of rhamnose, 29.55% of galactose, 19.00% of glucose, 4.47% of mannose and 7.22% of galacturonic acid. Experiments discover that the uncaria acidic polysaccharide URP-1 has remarkable anti-tumor activity on DU 145 cells, and a novel medicine raw material and a novel treatment method are provided for clinical treatment of prostatic cancer.

The invention discloses an anti-fatigue gastrodia elata and ginseng teabag as well as a preparation method and application thereof, and belongs to the technical field of functional food processing. The tea bag comprises the following components in percentage by mass: 30%-50% of gastrodia elata extract, 20%-30% of ginseng extract, 10%-20% of auxiliary herbal components and 12%-30% of carrier auxiliary materials, and is packaged by a degradable filter bag. Wherein the gastrodia elata extract is prepared through a composite enzymolysis-ultrasonic-assisted extraction method, the ginseng extract is prepared through extraction by adopting a low-temperature ultrasonic-ethanol precipitation combined process, and the two active ingredients are in synergistic interaction according to the mass ratio of the gastrodia elata extract to the ginseng extract being 1: (1.5-2). The freeze-drying process is adopted to retain effective components, and the degradable corn fiber / PLA filter bag is used for packaging, so that the stability and environmental protection property of the product are ensured. The traditional Chinese medicine is suitable for people with long-term mental or physical fatigue, and 1-2 bags of the traditional Chinese medicine can effectively reduce blood lactic acid accumulation every day.

The application discloses corn silk glucan, a structure formula of which is [→4)-β-D-Glcp-(1→]n, wherein n is 6-60, and a molecular weight range is 1-9.8 kDa. By extracting effective components in corn silk, an extract is obtained, the extract is precipitated by 30-90% ethanol, corn silk crude polysaccharide is obtained, and after the corn silk crude polysaccharide is dissolved, the corn silk crude polysaccharide is sequentially subjected to biological membrane dialysis, ion exchange resin column and HPLC-ELSD separation and purification, and corn silk glucan is obtained. The corn silk glucan and zeaxanthin are compounded to form a glucan compound, the glucan compound can stabilize insulin and improve the activity of the insulin in mouse islet cells, and the glucan compound has 2-5 times stronger hypoglycemic activity than that of zeaxanthin alone, and therefore, the glucan compound has important significance for researching new hypoglycemic drugs or health-care food for assisting hypoglycemia.

The invention discloses conductive leather based on a TGIC (triglycidyl isocyanurate) / multi-walled carbon nanotube chrome-free tanning agent. The conductive leather comprises a multi-walled carbon nanotube, gamma-glycidyl ether oxypropyl trimethoxy silane, triglycidyl isocyanurate and triethylamine, the preparation method comprises the following steps: performing amination modification on multi-walled carbon nanotubes through KH550, and uniformly dispersing the multi-walled carbon nanotubes under the action of a silane coupling agent; an epoxy group of triglycidyl isocyanurate and an amino group on the surface of the aminated carbon tube are subjected to a ring-opening cross-linking reaction, and a three-dimensional covalent network structure is constructed; and carrying out ethanol precipitation purification and drying treatment to obtain the chrome-free tanning agent. The traditional chromium salt is completely replaced, and the conductivity of the obtained leather is greater than 10 <-4 > S / cm; the mechanical property of the material is excellent, and the tensile strength exceeds 15 MPa; the material has excellent damp-heat resistance stability, and the structure is still stable after being treated at 100 DEG C for 24 hours; the production process is green and environment-friendly, and the cost is reduced by 40% compared with a traditional method. The method is especially suitable for manufacturing intelligent wearable equipment and flexible electronic sensors.

The invention relates to a preparation method of coprinus comatus selenium polysaccharide. Belongs to the field of pharmacy, and the international patent classification is C08B 37 / 00. The invention relates to a coprinus comatus selenium polysaccharide fine product, which is prepared by the following steps: crushing selenium-rich coprinus comatus mycelium obtained by deep liquid fermentation, sieving, carrying out ultrasonic-assisted extraction, ethanol precipitation, staying overnight at 4 DEG C, centrifuging, discarding supernatant, dissolving the precipitate in water, and deproteinizing by a Sevag method. And concentrating the supernatant, precipitating with ethanol, centrifuging to remove the supernatant, and drying to obtain a coprinus comatus selenium polysaccharide crude product. And purifying the coprinus comatus selenium polysaccharide crude product through ion exchange chromatography and gel filtration chromatography to obtain a coprinus comatus selenium polysaccharide refined product Se-CMP. The obtained coprinus comatus selenium polysaccharide is applied to preparation of drugs for protecting vascular endothelial cells and resisting cardiovascular and cerebrovascular system related diseases such as atherosclerosis.

The invention discloses a preparation method of a high-absorptivity calcium supplement of vitamin D3 (VD3) and casein phosphopeptide chelated calcium (CPP-Ca), which comprises the following steps: firstly, dissolving casein phosphopeptide (CPP) in ultrapure water, adding calcium chloride, adjusting the pH value, stirring and reacting at a certain temperature to generate CPP-Ca, then precipitating by using ethanol, washing for multiple times to remove free calcium ions, and drying to obtain the high-absorptivity calcium supplement of vitamin D3 (VD3) and casein phosphopeptide chelated calcium (CPP-Ca). Centrifuging, collecting the precipitate, and freeze-drying to obtain purified CPP-Ca; the preparation method comprises the following steps: preparing CPP-Ca into a solution, adjusting the pH value, dropwise adding VD3 dissolved in ethanol into the solution, stirring and mixing at room temperature under a certain concentration to finally form the VD3 and CPP-Ca compound, and the compound prepared by the method can significantly promote calcium absorption and effectively improve the stability of VD3 and CPP.