Method for comprehensively extracting tea seed saponin, tea seed polypeptide and tea seed polysaccharide from tea seed cake

A technology of tea seed saponin and tea seed polypeptide is applied in the preparation methods of peptides, chemical instruments and methods, peptides and other directions to achieve the effects of increasing added value, improving yield and purity

Inactive Publication Date: 2014-12-03
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report on the comprehensive preparation of tea...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The tea seed cake is crushed through a 300-400 mesh sieve, degreased with petroleum ether, added with absolute ethanol, the ratio of solid to liquid is 1:2 (g / mL), passed through a colloid mill (JM-LB) and a 40Mpa homogenizer (SHP4000 -25) 2 times each, extract in a water bath at 50°C for 2 hours, and extract 6 times. Centrifuge, take the supernatant and select an ultrafiltration membrane with a molecular weight cut-off of 2000Da for separation and purification, and the tangential flow rate is 4L / m 2 min, the pressure is 60psi, the retentate is purified tea seed saponin solution, concentrate until ethanol disappears completely, add hot water to dissolve, and freeze-dry. The obtained tea seed saponin has a yield of 92.1% and a purity of 90.3%;

[0025] Dry the tea seed meal after extracting tea seed saponin naturally, add water according to the ratio of material to liquid 1:2 (g / mL), adjust the pH to about 10.0 with 1MoL / L NaOH solution, and use ultrasonic-micro...

Embodiment 2

[0028] The tea seed cake is crushed through a 300-400 mesh sieve, degreased with petroleum ether, added with absolute ethanol, the ratio of solid to liquid is 1:4 (g / mL), passed through a colloid mill (JM-LB) and a 40Mpa homogenizer (SHP4000 -25) 2 times each, extract in a water bath at 50°C for 2 hours, and extract 6 times. Centrifuge, take the supernatant and select an ultrafiltration membrane with a molecular weight cut-off of 2000Da for separation and purification, and the tangential flow rate is 4L / m 2 min, the pressure is 60psi, the retentate is purified tea seed saponin solution, concentrate until ethanol disappears completely, add hot water to dissolve, and freeze-dry. The yield of tea seed saponin obtained was 96.3%, and the purity was 95.1%;

[0029] Dry the tea seed meal after extracting tea seed saponin naturally, add water according to the ratio of material to liquid 1:2 (g / mL), adjust the pH to about 10.0 with 1MoL / L NaOH solution, and use ultrasonic-mic...

Embodiment 3

[0032] The tea seed cake is crushed through a 300-400 mesh sieve, degreased with petroleum ether, then added with absolute ethanol, the ratio of solid to liquid is 1:6 (g / mL), and the colloid mill (JM-LB) and homogenizer (SHP4000- 25) Processing, extracting in a water bath at 50°C for 2 hours, and extracting 6 times. Centrifuge, take the supernatant and select an ultrafiltration membrane with a molecular weight cut-off of 2000Da for separation and purification, and the tangential flow rate is 4L / m 2 min, the pressure is 60psi, the retentate is purified tea seed saponin solution, concentrate until ethanol disappears completely, add hot water to dissolve, and freeze-dry. The obtained tea seed saponin has a yield of 89.1% and a purity of 90.1%;

[0033]Dry the tea seed meal after extracting tea saponin naturally, add water according to the ratio of material to liquid 1:2 (g / mL), adjust the pH to about 10.0 with 1MoL / L NaOH solution, and use ultrasonic-microwave double wa...

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PUM

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Abstract

The invention discloses a method for comprehensively extracting tea seed saponin, tea seed polypeptide and tea seed polysaccharide by using tea seed cake as a raw material. The extracting method comprises the steps of mixing degreased tea seed cake with ethanol, treating with a colloid mill and a homogenizer, then performing water bath ethanol extraction, centrifuging, performing ultra-filtration, and concentrating to obtain the tea seed saponin; mixing the centrifuged sediment with water, extracting with ultrasonic and microwave, centrifuging, regulating the pH of the supernate, centrifuging for the second time, dissolving the sediment, and performing enzymolysis, enzyme deactivation, ultra-filtration and spraying to obtain the tea seed polypeptide; and spraying the supernate obtained by secondary centrifuging to obtain coarse tea seed polysaccharide, and then performing ultra-filtration and ethanol precipitation to obtain the high-purity tea seed polysaccharide. The method is simple, low in production and equipment cost, high in product extraction rate and high in purity, and greatly improves the additional value of the tea seed cake. A new thought and a new method are provided for efficient comprehensive utilization of the tea seed cake.

Description

technical field [0001] The invention relates to a method for extracting bioactive substances from tea seed dregs. Background technique [0002] Tea seed saponin is also called tea saponin, tea saponin, a type of saponin. Studies have found that tea seed saponin has hemolysis and fish poisoning, anti-insect and bactericidal effects, anti-osmosis and anti-inflammation, phlegm and cough, analgesic, anti-cancer and other pharmacological functions. Tea seed saponin is also an excellent natural nonionic surfactant with strong foaming, emulsifying, dispersing, and moisturizing effects. It can be widely used in daily chemicals, building materials, textiles, pesticides and other fields, and has high economic value. [0003] Tea seed polypeptide is easily digested and absorbed by the human body, and has the physiological functions of anti-oxidation, lowering blood pressure, lowering cholesterol content, and promoting calcium absorption. Tea seed polysaccharide is a polymer compound...

Claims

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Application Information

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IPC IPC(8): C08B37/00C07H15/256C07H1/08C07J63/00C12P21/06C07K1/34
Inventor 涂宗财黄涛王辉李如一段邓乐
Owner NANCHANG UNIV
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