The invention relates to an eDNA method for identifying
bumblebee honeycombs in
permafrost regions of Qinghai-Tibet
Plateau. The eDNA method comprises the following steps: S1, positioning observation sample belts and arranging sampling points on the basis of multi-
source data in combination with a Bayesian hierarchical model; s2, performing
deep freezing and complete excavation on matrixes around and inside all the sampling points; s3,
stratified sampling and anti-degradation treatment are carried out, and a spatial attribute
database is established; s4, extracting and purifying eDNA (enhanced deoxyribonucleic acid); s5, determining a
nest host based on PCR amplification, high-
throughput sequencing and sequence comparative analysis; and S6, constructing a Bayesian hierarchical model in combination with biological information, and judging
honeycomb characteristics. The method is simple, rapid and low in cost, not only is suitable for Qinghai-Tibet
Plateau permafrost regions, has the core advantages of non-destructiveness and high sensitivity, but also is also suitable for underground
insect nest identification research in other environment sensitive regions or regions difficult to physically explore in principle, and has wide application prospects. The method is especially suitable for monitoring rare, secret or difficult-to-approach organisms.