43 results about "Silencing Elements" patented technology

Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Coleopteran plant pest or a Diabrotica plant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: 1-236 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is D. virgifera virgifera, D. barberi, D. speciosa, or D. undecimpunctata howardi. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Pentatomidae plant pest or a N. viridula, Acrosternum hilare, Piezodorus guildini, and/or Halymorpha halys plant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: 1-292 or 302-304 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Pentatomidae. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Lygus genus, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides from specific polypeptide families as disclosed herein, and further provides target polynucleotides set forth in SEQ ID NOS:1-21 or active variants thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Lygus Hesperus. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

Apparatus (10, 200) for the provision of TNP therapy to a wound is described, the apparatus (10, 200) including: a waste canister (22, 204) into which fluid is aspirated from a wound by aspiration pump means (44, 248) and a fluid flow path (420, 422) for at least a part of said aspirated fluid on an outlet side of said aspiration pump means (44) wherein said fluid flow path (420, 422) on the outlet side comprises a silencing system (400) having a plurality of silencing elements (402, 404, 406) therein.

Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Aphididae family, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides target polynucleotides for specific protein classes and also target polynucleotides as set forth in SEQ ID NOS:1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or active variants or fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Aphis glycines. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

This invention discloses a motor structure of a washing machine capable of reducing noise and shake including a rotating shaft, a magnet, a stator, a rotation shaft frame and a silencing element, among which, said magnet is set at the inter-circle face of the frame side wall, said stator is set in the shaft frame, the silencing element is set on the peripheral face of the rotation shaft frame.

Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Pentatomidae plant pest, decrease the expression of a target sequence in the pest. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: 6-12, 18-40 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

The present application describes a plant gene regulatory element and the uses thereof. More particularly a silencing element for modulating plant responses to pathogens, auxin and ethylene is described. The invention also describes transcriptional repressors which specifically binds onto the silencing element of the invention. Nucleotide and amino acid sequences of a novel transcriptional repressor referred as 'SEBF' are given.

Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Coleopteran plant pest or a Diabrotica plant pest, decrease the expression of a target sequence in the pest. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: disclosed herein, (but not including the forward and reverse primers.) or active variants and fragments thereof, or complements thereof, wherein a decrease in expression of one or more of the sequences in the target pest controls the pest (i.e., has insecticidal activity). Plants, plant parts, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

Compositions and methods are provided which employ a chemical-gene switch. The chemical-gene switch disclosed herein comprises at least three components. The first component comprises a polynucleotide encoding a chemically-regulated transcriptional repressor; the second component comprises a repressible promoter operably linked to a polynucleotide of interest, and the third component comprises a gene silencing construct operably linked to a second repressible promoter, wherein the gene silencing construct encodes a silencing element that decreases the level of mRNA encoding the chemically -regulated transcriptional repressor. Expression of the polynucleotide of interest and the silencing construct is controlled by providing the appropriate chemical ligand. Transient induction from the chemical ligand leads to the production of the silencing element, and the destruction of the mRNA encoding the chemically-regulated transcriptional repressor. The presence of the silencing element maintains a state of de-repression. In specific embodiments, the silencing elements are cell non-autonomous, the state of de-repression becomes more distributed throughout the plant beyond where the chemical ligand reaches.

The invention discloses a cross-flow cooling tower additionally provided with a silencing device. The cross-flow cooling tower comprises a tower body, and a filler structure, a spraying structure and an air suction structure which are fixed in the tower body, wherein the tower body is provided with a side air inlet and a top air outlet which are communicated with each other, the filler structure is arranged below the spraying structure, and the air suction structure is arranged below the top air outlet; a first silencing device is arranged at the side air inlet, and a second silencing device is arranged at the top air outlet; and the first silencing device comprises at least two rows of first silencing elements which are arranged in a staggered mode, and the second silencing device comprises a plurality of second silencing elements which are arranged in an inclined mode. The cooling tower not only can reduce the water temperature, but also can reduce the installing space only by considering the single-side ventilation requirement, and is particularly suitable for being installed on a site with good single-side ventilation and poor other-side ventilation. Meanwhile, the cooling tower is additionally provided with the silencing device, so that the influence of noise on the surrounding environment can be reduced, and the cooling tower is particularly suitable for being installed in places close to sensitive points or with noise requirements.

The invention discloses a manufacturing method of a silencing element used in front of a carbon tank electromagnetic valve. The manufacturing method comprises the following steps that 1, a silencer body and a fixing structure are correspondingly manufactured by adopting different materials, the material rigidity of the silencer body is greater than the material rigidity of the fixing structure, or/and the material toughness of the fixing structure is greater than the material toughness of the fixing structure; and 2, the fixing structure is assembled on the surface of the silencer body. The silencing element manufactured by the manufacturing method can meet the rigidity required by the silencer body and the toughness required by the fixing structure at the same time.