The application belongs to the technical field of
genetic engineering and
plant genetic breeding, and particularly relates to application of a new tetraploid rice
fertility gene NTFR2. The application uses a
gene knockout technology to determine the function of the
fertility regulation
gene NTFR2 in the new tetraploid rice, and finds that the NTFR2 affects the
fertility of the new tetraploid rice. When the function of the NTFR2 gene is lost, the seed setting rate of the
mutant is reduced, and the development of the female
gametophyte sac exhibits
abnormality. Meanwhile, the loss of the function of the NTFR2 gene leads to a significant decrease in the fertility of the new tetraploid rice, but has a smaller influence on the male gametes and other agronomic traits, which indicates that the NTFR2 is a new gene mainly affecting the fertility of the tetraploid rice, and has an important application prospect in the fields of creating a new female sterile line and further promoting the utilization of
hybrid vigor of the tetraploid rice.