The invention belongs to the field of seaweed
molecular genetics, and relates to an enteromorpha gender specific
molecular marker and application thereof. The enteromorpha gender specific
molecular marker is characterized in that one enteromorpha female
gametophyte DNA specific
molecular marker and one enteromorpha male
gametophyte DNA specific molecular marker are provided, wherein the nucleotidesequences of the enteromorpha female
gametophyte DNA specific molecular marker and the enteromorpha male gametophyte DNA specific molecular marker are respectively SEQ ID NO. 3 and SEQ ID NO. 4, theSEQ ID NO. 3 is obtained by amplifying a primer sequence SEQ ID NO. 1, and the SEQ ID NO. 4 is obtained by amplifying a primer sequence SEQ ID NO. 2. The specific molecular marker is completed by detecting the specific fragment through PCR (
polymerase chain reaction), and the process comprises the following steps of extracting to-be-detected enteromorpha
genomic DNA, performing PCR amplification,and performing
agarose gel electrophoresis detection on an amplification product. The specific primer is obtained by referring to existing high-
throughput data result in a laboratory, combining genomeDNA extraction, target fragment amplification and
recovery,
cloning by using pGEM-
T vector and
escherichia coli competent strain TOP10 and the like. By means of the specific molecular marker, ploidyof a large number of enteromorpha
algae and gender of gametophytes can be rapidly, simply and accurately identified, and an important molecular tool is provided for enteromorpha
life history researchand variety breeding.