11 results about "Female Gametophytes" patented technology

Provided herein are methods and compositions for inducing parthenogenesis by expression in female gametophytes of a Babyboom (BBM) polynucleotide operably linked to a promoter expressed in an egg cell. Also included herein are promoters expressed in egg cells of plants and methods of using these promoters to express gene products in egg cells of plants.

The invention discloses a method for cultivating kelp seedlings through diploid female gametophyte parthenogenesis, and belongs to the field of aquatic product engineering genetic breeding. And hybridizing the female and male single cell cloning lines of the induced protonema to obtain the tetraploid sporophyte. And the tetraploid sporophyte is mature and releases zoospores, diploid gametophytes are germinated and formed, and then male and female separation is performed to obtain diploid female gametophyte clone lines in batches. Under the culture condition provided by the invention, parthenogenesis of a diploid female gametophyte clone line is utilized for seedling culture. The diploid female gametophyte begins to ovulate after 6-7 days, and the ovulation time of the diploid female gametophyte is obviously earlier than that of haploid gametophyte in the same period and under the same condition. The 12-day ovulation rate reaches 86.5%, the 24-day sporophyte conversion rate reaches 98.3%, and most sporophyte forms are normal. In the seedling raising period, parthenogenesis seedling raising of the diploid female gametophyte is completed. The diploid female gametophyte can be used as a brand-new kelp germplasm material to be applied to kelp seedling cultivation.

The invention provides a specific molecular marker for sex identification of male and female gametophytes of gracilaria and application of the specific molecular marker. The specific molecular marker of the female gametophytes of gracilaria is GvF-06, and the nucleotide sequence of the specific molecular marker is shown as SEQ ID NO.1; the specific molecular marker of the gracilaria male gametophyte is GvM-03, and the nucleotide sequence of the specific molecular marker is as shown in SEQ ID NO. 2. By utilizing the specific molecular marker, the sex of male and female gametophytes of gracilaria can be quickly and accurately identified, an important molecular tool is provided for genetic breeding work of gracilaria, and the specific molecular marker has important economic benefits and is suitable for popularization and application.

The present application relates to the field of forest tree seedling breeding biotechnology, and specifically to a method for improving the induction rate of embryonic callus of Pinus elliottii or Pinus elliottii, comprising picking cones that meet preset conditions from a donor plant, separating seeds from the cones, peeling off female gametophytes from the seeds, and inducing and culturing the female gametophytes using somatic embryogenesis technology; the preset conditions include: (1) the donor plant is in the induction harvest period; (2) the development status of the picked cones is qualified; the present application solves the problems of poor repeatability and failure of somatic embryogenesis caused by misunderstandings in the traditional method in the judgment of embryonic callus induction and the calculation of induction rate, and provides an important reference for the large-scale production of somatic embryo seedlings of Pinus elliottii or Pinus elliottii based on somatic embryogenesis technology.

Methods and compositions used to prevent or decrease reduction, or reduction and recombination, during meiosis in female sporocyte, female gametophytes, or female gametes are described herein. The compositions and methods may include silencing elements and / or use gene-editing technology to decrease or inhibit Nrf4 expression levels or activity. Further provided are compositions and methods for the production of a plant that has ovules comprising non-reduced, or non-reduced and non-recombined, gametes, and / or seeds that contain clonal maternal embryos.

The invention discloses a genetically engineered kelp-based oral mucosal immune activation type HPV (human papilloma virus) vaccine as well as a preparation method and application thereof, and belongs to the technical field of manufacturing of genetically engineered vaccines. A kelp female gametophyte is modified by a genetic engineering method, parthenogenesis of the kelp female gametophyte is induced, a kelp plant stably expressing HPV L1 protein is obtained, and the oral mucosal immune activated HPV vaccine is prepared. According to the HPV vaccine provided by the invention, natural polysaccharide of kelp is used as an adjuvant and is absorbed through intestinal mucosa, so that the adverse reaction caused by traditional vaccine injection can be reduced while the mucosa immune reaction can be effectively activated. By utilizing the characteristic of rapid growth of the kelp and the advantage that the prepared vaccine does not need cold chain transportation, low-cost production and large-range coverage of the HPV vaccine can be realized, and global prevention and eradication of cervical cancer are expected to be promoted.

The invention relates to an OsEPF1 gene for regulating and controlling generation of rice macrospores, in particular to participation of a plant secretory peptide OsEPF1 rich in cysteine in regulating and controlling the generation process of the rice macrospores. Compared with wild type ZH11, the OsEPF1 gene function deletion mutant for regulating and controlling rice macrospore generation has obvious defects in female germ cell development, especially in the early ovule development stage and the AC and MMC stages, the mutant can generate a plurality of AC / MMC-like cells, then fertility is affected, and the fruiting rate of a rice single plant is reduced; the pEPF1:: EPF1 is supplemented back into the mutant postovule, the growth process of the macrospore is normal, and the maturing rate can be recovered to be normal. The invention proves that the OsEPF1 affects the development of female gametophytes and finally affects the maturing rate of rice by regulating the transformation from somatic cells to germ cell fate in the rice macrospore generation process, and the discovery has important significance on the research on the determination of the rice female germ cell fate, and has certain application value on rice breeding work.

The application belongs to the technical field of genetic engineering and plant genetic breeding, and particularly relates to application of a new tetraploid rice fertility gene NTFR2. The application uses a gene knockout technology to determine the function of the fertility regulation gene NTFR2 in the new tetraploid rice, and finds that the NTFR2 affects the fertility of the new tetraploid rice. When the function of the NTFR2 gene is lost, the seed setting rate of the mutant is reduced, and the development of the female gametophyte sac exhibits abnormality. Meanwhile, the loss of the function of the NTFR2 gene leads to a significant decrease in the fertility of the new tetraploid rice, but has a smaller influence on the male gametes and other agronomic traits, which indicates that the NTFR2 is a new gene mainly affecting the fertility of the tetraploid rice, and has an important application prospect in the fields of creating a new female sterile line and further promoting the utilization of hybrid vigor of the tetraploid rice.

The invention discloses a method for rapidly propagating solieria seedlings, and relates to the technical field of solieria cultivation. The invention provides a method suitable for rapid propagation of solieria seedlings in a land-based artificial regulation environment. The whole set of method combines a first-stage propagation process and a second-stage propagation process. When primary propagation is carried out, immature quartophyte or male and female gametophyte fronds are used as propagation mother branches, a plurality of independent offspring seeds are obtained after cleaning, cutting and seedling separation treatment, then the cut independent offspring seeds are inoculated into a culture container of an algae culture chamber for suspension aeration culture, and the offspring seeds are promoted to recover, germinate and grow; during second-stage propagation, the solieria seedlings subjected to first-stage propagation are inoculated into a floating frame with holes and placed in a circulating water seedling propagation pool for rapid propagation. The whole propagation process is completed in a land-based artificial regulation environment, and the method has the advantages of being high in propagation efficiency, short in cultivation period, long in suitable cultivation period, high in seedling survival rate and budding rate and the like.

The present invention discloses a method for cultivating kelp seedlings using parthenogenesis of diploid female gametophytes, belonging to the field of genetic breeding in aquatic engineering. Female and male single-cell clones of induced conchoderma are hybridized to obtain tetraploid sporophytes. The tetraploid sporophytes mature and release zoospores, which germinate to form diploid gametophytes. Male and female gametophytes are then separated and batches of diploid female gametophyte clones are obtained. Under the culture conditions provided by the present invention, seedlings are cultivated using parthenogenesis of the diploid female gametophyte clones. These diploid female gametophytes begin to ovulate within 6 to 7 days, significantly earlier than the ovulation time of haploid gametophytes under the same conditions during the same period. The ovulation rate reaches 86.5% on day 12, and the sporophyte conversion rate reaches 98.3% on day 24. Most of the sporophytes have normal morphology. During the seedling cultivation period, the diploid female gametophytes complete parthenogenesis. The diploid female gametophytes can be used as a new kelp germplasm material for kelp seedling cultivation.

The invention relates to a receptor-like kinase OsERL gene for regulating and controlling development of rice female gametophytes and participation of the receptor-like kinase OsERL gene in regulation and control of development of rice female gametophytes. Compared with a wild type ZH11, the OsERL gene function deletion mutant for regulating and controlling the development of the rice female gametophyte has the advantages that the development of female germ cells has obvious defects, so that the fruiting rate of a single rice plant is reduced; the pERL:: ERL is supplemented back to the mutant postovule, the female gametophyte develops normally, and the maturing rate can be recovered to be normal. The research proves that the OsERL influences the maturing rate of the rice by regulating and controlling the development of the rice female gametophyte, and the discovery has important significance on the research on the fate decision of the rice female germ cells and has certain application value on the rice breeding work.