6 results about "Female Gametophytes" patented technology

The invention discloses a method for cultivating kelp seedlings through diploid female gametophyte parthenogenesis, and belongs to the field of aquatic product engineering genetic breeding. And hybridizing the female and male single cell cloning lines of the induced protonema to obtain the tetraploid sporophyte. And the tetraploid sporophyte is mature and releases zoospores, diploid gametophytes are germinated and formed, and then male and female separation is performed to obtain diploid female gametophyte clone lines in batches. Under the culture condition provided by the invention, parthenogenesis of a diploid female gametophyte clone line is utilized for seedling culture. The diploid female gametophyte begins to ovulate after 6-7 days, and the ovulation time of the diploid female gametophyte is obviously earlier than that of haploid gametophyte in the same period and under the same condition. The 12-day ovulation rate reaches 86.5%, the 24-day sporophyte conversion rate reaches 98.3%, and most sporophyte forms are normal. In the seedling raising period, parthenogenesis seedling raising of the diploid female gametophyte is completed. The diploid female gametophyte can be used as a brand-new kelp germplasm material to be applied to kelp seedling cultivation.

The invention provides a specific molecular marker for sex identification of male and female gametophytes of gracilaria and application of the specific molecular marker. The specific molecular marker of the female gametophytes of gracilaria is GvF-06, and the nucleotide sequence of the specific molecular marker is shown as SEQ ID NO.1; the specific molecular marker of the gracilaria male gametophyte is GvM-03, and the nucleotide sequence of the specific molecular marker is as shown in SEQ ID NO. 2. By utilizing the specific molecular marker, the sex of male and female gametophytes of gracilaria can be quickly and accurately identified, an important molecular tool is provided for genetic breeding work of gracilaria, and the specific molecular marker has important economic benefits and is suitable for popularization and application.

Methods and compositions used to prevent or decrease reduction, or reduction and recombination, during meiosis in female sporocyte, female gametophytes, or female gametes are described herein. The compositions and methods may include silencing elements and / or use gene-editing technology to decrease or inhibit Nrf4 expression levels or activity. Further provided are compositions and methods for the production of a plant that has ovules comprising non-reduced, or non-reduced and non-recombined, gametes, and / or seeds that contain clonal maternal embryos.

The application belongs to the technical field of genetic engineering and plant genetic breeding, and particularly relates to application of a new tetraploid rice fertility gene NTFR2. The application uses a gene knockout technology to determine the function of the fertility regulation gene NTFR2 in the new tetraploid rice, and finds that the NTFR2 affects the fertility of the new tetraploid rice. When the function of the NTFR2 gene is lost, the seed setting rate of the mutant is reduced, and the development of the female gametophyte sac exhibits abnormality. Meanwhile, the loss of the function of the NTFR2 gene leads to a significant decrease in the fertility of the new tetraploid rice, but has a smaller influence on the male gametes and other agronomic traits, which indicates that the NTFR2 is a new gene mainly affecting the fertility of the tetraploid rice, and has an important application prospect in the fields of creating a new female sterile line and further promoting the utilization of hybrid vigor of the tetraploid rice.

The invention discloses a method for rapidly propagating solieria seedlings, and relates to the technical field of solieria cultivation. The invention provides a method suitable for rapid propagation of solieria seedlings in a land-based artificial regulation environment. The whole set of method combines a first-stage propagation process and a second-stage propagation process. When primary propagation is carried out, immature quartophyte or male and female gametophyte fronds are used as propagation mother branches, a plurality of independent offspring seeds are obtained after cleaning, cutting and seedling separation treatment, then the cut independent offspring seeds are inoculated into a culture container of an algae culture chamber for suspension aeration culture, and the offspring seeds are promoted to recover, germinate and grow; during second-stage propagation, the solieria seedlings subjected to first-stage propagation are inoculated into a floating frame with holes and placed in a circulating water seedling propagation pool for rapid propagation. The whole propagation process is completed in a land-based artificial regulation environment, and the method has the advantages of being high in propagation efficiency, short in cultivation period, long in suitable cultivation period, high in seedling survival rate and budding rate and the like.

The present invention discloses a method for cultivating kelp seedlings using parthenogenesis of diploid female gametophytes, belonging to the field of genetic breeding in aquatic engineering. Female and male single-cell clones of induced conchoderma are hybridized to obtain tetraploid sporophytes. The tetraploid sporophytes mature and release zoospores, which germinate to form diploid gametophytes. Male and female gametophytes are then separated and batches of diploid female gametophyte clones are obtained. Under the culture conditions provided by the present invention, seedlings are cultivated using parthenogenesis of the diploid female gametophyte clones. These diploid female gametophytes begin to ovulate within 6 to 7 days, significantly earlier than the ovulation time of haploid gametophytes under the same conditions during the same period. The ovulation rate reaches 86.5% on day 12, and the sporophyte conversion rate reaches 98.3% on day 24. Most of the sporophytes have normal morphology. During the seedling cultivation period, the diploid female gametophytes complete parthenogenesis. The diploid female gametophytes can be used as a new kelp germplasm material for kelp seedling cultivation.