A method of constructing double haploid (dh) sporophyte populations in Wakame

A sporophyte and wakame technology, applied in the field of genetic breeding, can solve the problems that have not yet been reported on DH populations

Active Publication Date: 2022-06-07
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

The DH population is widely used in the genetics and genetic breeding research of higher plants. Due to the differences in the life history of various seaweed species, although it has also been established and applied in the economic red alga Porphyra, there has been no report on the DH population in brown algae.

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  • A method of constructing double haploid (dh) sporophyte populations in Wakame
  • A method of constructing double haploid (dh) sporophyte populations in Wakame
  • A method of constructing double haploid (dh) sporophyte populations in Wakame

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Embodiment 1

[0040] like figure 1 shown, select F for parthenogenesis 1 Cloning of female gametophytes and F that can produce hermaphroditism 1 Male gametophyte clones, the genetic relationship analysis of the 10 SSR loci of the selected gametophyte clones showed that the female and male gametophyte clones had different alleles (80% degree of difference) at 8 loci, indicating that the two The genotypes have obvious differences and are suitable as parents for group construction. The female and male gametophyte clones were mixed in a mass ratio of 1:1, and then hybridized to obtain F. 1 Hybrid sporophyte; the hybridization process is the mixing of female and male gametophyte clones in PES seawater medium at a temperature of 18 °C and a light intensity of 50 μmol photons m -2 s -1 , photoperiod 12h day: 12h night; and cultured for 30 days under the condition of changing the medium every 3 days, the resulting F 1 When the sporophyte reaches more than 1mm, it is transferred to a 2L beaker,...

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Abstract

The present invention relates to genetic breeding, specifically a method for constructing doubled haploid (DH) sporophyte populations in Undaria pinnatifida, and further to obtain Undaria pinnatae by using parthenogenesis and selfing of hermaphrodite gametophytes Methods for DH sporophyte populations. Select female gametophyte clones with parthenogenetic ability and male gametophyte clones that can produce hermaphroditic phenomena, and cross the two to produce F 1 sporophyte, after it matures, select a strain to release zoospores, and obtain a large amount of F 2 Gametophyte clone; select the female gametophyte clone among them, obtain the female DH sporophyte strain by inducing the occurrence of parthenogenesis, select the male gametophyte clone among them, and obtain the male DH sporophyte strain by inducing the occurrence of hermaphroditism and selfing, Thereby obtaining DH sporophyte population. The DH population constructed by the invention is of great significance for basic genetics research and new variety cultivation in important economical brown algae Undaria pinnatae.

Description

technical field [0001] The invention relates to genetic breeding, in particular to a method for constructing a DH sporophyte population in wakame, and furthermore, a method for obtaining a wakame DH sporophyte population by utilizing parthenogenesis and hermaphroditic gametophyte selfing. Background technique [0002] Wakame is an important economic brown algae in my country. It belongs to the kelp order and has a typical life history of the kelp brown algae. Most of the important agronomic traits of wakame are quantitative traits, and the genetic basis of quantitative traits needs to be analyzed to carry out genetic breeding research. The construction of a mapping population is one of the prerequisites for quantitative trait genetic research, but the current mapping populations in Wakame and even the entire kelp brown algae are basically temporary common F. 2 The population cannot be preserved for a long time, it is difficult to carry out repeated studies at different tim...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04A01H4/00
CPCA01H1/02A01H1/04A01H4/001A01H4/008Y02A40/80
Inventor 单体锋逄少军王雪妹李静
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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