5 results about "Hermaphrodite" patented technology

The invention provides an ictalurus punctatus sex regulation target, a zbtb38 gene knockdown ictalurus punctatus sex reversal method and application, and belongs to the technical field of sex regulation. According to the invention, the sex control effective regulation target spot zbtb38 gene is successfully screened out, and the shRNA target spot is designed aiming at the coding region of the channel catfish zbtb38 gene, so that compared with CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats), the shRNA target spot has the characteristics of easiness in death and low success rate, and the expression inhibition of the channel catfish zbtb38 gene is effectively realized through site-directed gene knock-down. According to the method, the ietalurus punetaus sex reversal model is successfully established, all-female reversed fishes and hermaphrodite reversed fishes are obtained, the method has important value for sex determination mechanism research of the ietalurus punetaus, optimization of an all-male breeding strategy can be reversely guided, a scientific basis is provided for all-male group breeding and breeding cost optimization, and the method has wide application prospects. And finally, the high-quality development of the ictalurus punctatus breeding industry is promoted.

The present application belongs to the technical field of preparation of excellent commercial seed of economic marine shellfish, and particularly relates to a high-efficiency method for preparing interspecific three-way hybrid of Argopecten genus scallop. The method uses a selected and purified male sterile and female fertile hybrid Argopecten mactrolineatus as a female parent, and a restocked Argopecten purpuratus as a male parent, to produce a "Argopecten mactrolineatus Argopecten purpuratus" three-way hybrid scallop commercial seed with hybrid vigor and outstanding stress resistance. The method can realize large-scale, continuous and efficient hybrid seed production, and has the advantages of simple operation, labor saving, low dependence on personnel, and the like. The hybridization rate is increased from less than 30% in the prior art to more than 90%, and the efficiency of interspecific hybrid seed production and the purity of the seed of the protandrous Argopecten genus scallop are significantly improved.

The present invention relates to a Vitis sp. plant or part thereof having a reduced level of flower sex polypeptide (FSL) activity compared to a corresponding wild-type Vitis sp. plant or part thereof having an FSL locus genotype that confers a hermaphrodite flower phenotype, wherein said Vitis sp. plant or part thereof comprises an FSL locus comprising a polynucleotide sequence encoding the FSL polypeptide, and wherein the polynucleotide sequence comprises a genetic modification in the region encoding a plant domain with an AT₂-rich zinc-binding sequence (PLATZ) of the FSL polynucleotide in relation to a corresponding polynucleotide sequence of a wild-type Vitis sp. FSL locus allele that confers a hermaphrodite flower phenotype when expressed, whereby the genetic modification causes a male reproductive part of a flower in the Vitis sp. plant to be expressed.or part of it is not functional.

The application belongs to the field of biotechnology and genetic engineering, and discloses a culture method of double-fluorescently labeled Caenorhabditis elegans for monitoring mitochondrial homeostasis, which comprises the following steps: culturing Caenorhabditis elegans ATU3301 strain to L4 larva stage, and selecting male as the father; culturing Caenorhabditis elegans MQD753 strain to L4 larva stage, and selecting hermaphrodite as the mother; placing them on a culture plate in a certain proportion, mating, and obtaining offspring; removing the parents and continuing to culture the offspring to L2 larva stage; screening Caenorhabditis elegans through a microscope to obtain target Caenorhabditis elegans; without an external staining agent, the fluorescent protein is stably integrated into the genome and specifically located in mitochondria, the signal is stable, the location is accurate, and the repeatability is high, which provides a new tool with physiological relevance, operational convenience and cost advantage for the research on mitochondrial homeostasis mechanism and the development of related disease drugs, and has great potential application value.

The invention provides a papaya Y linkage region specific serial insertion SV molecular marker and application thereof, and belongs to the technical field of molecular biology, the SV molecular marker is HSY3-TR-13bp, and is used for identifying the gender of papaya; the primer pair is used for detecting the SV molecular marker; the hybridization probe is used for detecting the SV molecular marker; preparing a kit and a hybridization chip by using the primer pair or the hybridization probe; the primer pair, the hybridization probe, the kit or the hybridization chip are used for identifying the gender of the papaya. According to the specific haplotype SV marker, rapid sex identification of the amphoteric plants in the seedling stage can be achieved, the identification accuracy of the HSY3 type (belonging to dominant haplotype in market cultivated species) amphoteric plants is 100%, the screening efficiency of the seedlings of the amphoteric plants in the papaya seedling breeding process is improved, the orchard planting density is increased, and the labor management cost, the fertilizer cost and the like are reduced.