98 results about "Catfish" patented technology

The invention discloses a treatment method for fermenting smelly mandarin fish by coupling gradient salt control and directional inoculation, which comprises the following steps of: pretreating the smelly mandarin fish, putting the smelly mandarin fish into ozone-containing water for bacteria reduction treatment, then putting the smelly mandarin fish into a salt solution for pickling, finally inoculating bacteria liquid into the pickled smelly mandarin fish for fermentation treatment, cleaning, draining, packaging and quickly freezing after fermentation is finished, and thus obtaining the smelly mandarin fish. According to the method, the fermentation production process of the smelly mandarin fish is adjusted and standardized, the microorganism content of the slaughtered smelly mandarin fish is reduced through pretreatment, then the bacteria-reduced smelly mandarin fish is pickled with a low-concentration sodium chloride aqueous solution, meanwhile, the screened strains (psychrobacter halophilus and lactobacillus plantarum) are inoculated, and compared with traditional fermentation, the method is easy to operate, low in cost and high in yield. The product quality is stable, the defective rate is low, the labor cost is saved, the speed is high, the volatile basic nitrogen of the obtained smelly mandarin fish is less than or equal to 30mg / 100g, and the peroxide value (in terms of fat) is less than or equal to 2.5 g / 100g.

The invention relates to the technical field of aquaculture methods, in particular to a breeding method for preventing and treating ichthyophthiriasis of leiocassis longirostris fries. According to the technical scheme, the first system and the second system are adopted for sequential circulation treatment, and finally the third system is adopted for treatment, so that prevention and treatment of ichthyophthiriasis are achieved. The three systems are all subjected to heating treatment, and the first system contains sodium chloride and formaldehyde, so that rapid falling of insect bodies is promoted, and development of larvae is inhibited; the second system contains povidone iodine, so that body surface infection can be prevented; the third system consolidates the treatment effect; in the treatment process, unhatched cysts and dead polypides are discharged by changing water. A physical heating measure is adopted, so that osmotic stress of salinity on the freshwater fish is reduced. The adopted medicines are all easy to metabolize, are free of heavy metal ions, and have ideal ecological safety and food safety. According to the technical scheme, the technical problem that in the prior art, a safe and effective breeding method for preventing and treating the Leiocassis longirostris ichthyophthiriasis is lacked can be solved, and the method has ideal application and popularization prospects.

The invention discloses a catfish particle swarm optimization algorithm-based wind generating set parameter identification method. The method comprises the following steps of: constructing a direct-driven and double-fed wind generating set model in a PSCAD (Power System Computer Aided Design); adjusting control parameters of the built direct-driven and doubly-fed wind generating set identification model to obtain output data of the wind generating set; classifying the parameters according to the parameter sensitivity; constructing an error model of the target model and the identification model for the high-sensitivity parameters; and coding the control parameters, and carrying out iterative optimization operation on the control parameters by adopting a catfish particle swarm optimization algorithm to obtain an identification result of the control parameters of the direct-driven and double-fed wind generating sets. According to the method, the particle swarm optimization algorithm introducing the catfish operator is utilized, so that particles are prevented from falling into local optimum in parameter identification, the convergence speed of the particles is increased, the iterative optimization speed and effect of the algorithm are improved, and reliable identification of the control parameters of the direct-driven and doubly-fed wind generating sets can be realized.

The invention discloses a low-stress bleeding processing method for channel catfish. The method comprises the following steps: an acid-base control step, in which the channel catfish is placed in water containing sodium citrate to control the acid-base in the body, thereby increasing the alkali reserve in the body of the fish; a combined light anesthesia step, in which the channel catfish is lightly anesthetized through the combined action of low temperature and a chemical anesthetic, so that the fish exhibits a state in which the gill cover of the fish opens and closes weakly and slowly, and the fish body rolls over and loses balance; a fish body puncture step, in which the blood circulation of the channel catfish is destroyed by a manual or mechanical method, thereby creating a bleeding wound on the fish body; a metabolic bleeding step, in which the fish body is placed in a bleeding tank that has been aerated and oxygenated in advance to saturated dissolved oxygen, for bleeding, while maintaining a certain dissolved oxygen concentration; and an ultrasonic blood purification step, in which the fish body is placed in a bleeding tank equipped with an ultrasonic device for bleeding, thereby accelerating the discharge of residual blood in the body.

The invention discloses a channel catfish gill bloodletting point self-adaptive positioning mechanism which comprises a horizontal sliding block seat in transmission connection with a driving component and driven by the driving component to move front and back. The tool apron is connected with the horizontal sliding block base in a left-right sliding mode and can conduct self-adaptive movable adjustment relative to the horizontal sliding block base, the sliding direction of the tool apron is perpendicular to the sliding direction of the horizontal sliding block base, spring bases are fixedly connected to the left side and the right side of the horizontal sliding block base correspondingly, and spring sets are connected to the left side and the right side of the tool apron correspondingly. The left side / right side of the tool apron is elastically connected with the spring seat on the left side / right side through a spring group; the cutter module is used for puncturing a fish body and can be adjusted in a self-adaptive mode, and the cutter module is rotatably, movably and adjustably connected with the cutter holder. By adopting the scheme of the invention, the problems of gill bloodletting point identification and positioning in the prior art can be solved.

The invention discloses an SSR (Simple Sequence Repeat) primer composition for evaluating genetic diversity of channel catfish breeding population and an application method of the SSR primer composition, and belongs to the technical field of fish genetic breeding. The method comprises the following steps: modifying 5'ends of forward primers of the primers by using FAM fluorophores respectively, amplifying genome DNA of each breeding group by using the 10 pairs of microsatellite site primers respectively, analyzing amplification products, reading genotypes, analyzing parameters, and determining the genotype of the breeding group. The analysis result is used for evaluating the population polymorphism, the variation condition of the population genetic structure and the genetic differentiation degree among the populations. By applying the evaluation method disclosed by the invention, the population genetic diversity and genetic structure of continuous breeding generations of channel catfish can be systematically evaluated. The SSR primer and the genetic diversity evaluation method can be used for genetic improvement and new variety breeding of channel catfish.

The utility model provides a sewage suction device for a land-based barrel culture pond of mastacembelus armatus, which comprises a culture pond, a first sewage suction pipe, a second sewage suction pipe, a collecting box, a third sewage suction pipe and a filter, the bottom of the culture pond is in an inverted cone shape, one end of the first sewage suction pipe is connected with the filter, and the other end of the first sewage suction pipe extends to the lowest point of the bottom of the culture pond; one end of the second sewage suction pipe is connected with the filter, the other end of the second sewage suction pipe is connected with an inner cavity of the collecting box, and the bottom of the collecting box is 20-30 cm lower than the water level of the culture pond; one end of the third sewage suction pipe is connected with the bottom of the collection box. As the sealing cover for sealing is arranged on the collecting box, negative pressure can be formed after water in the collecting box is pumped by the water pump, water flows into the collecting box in an accelerated mode through the third sewage suction pipe, the suction force of the third sewage suction pipe is improved, the higher sewage suction capacity is obtained, sewage at the bottom of a culture pond and diseased and dead fishes can be better sucked into the collecting box, and therefore the quality of the culture pond is improved. The water quality influence caused by rotting of diseased and dead fishes is avoided.

The utility model provides a trachinotus ovatus culture net cage which comprises a feeding device and a moving device, the feeding device comprises a motor, a first rotating shaft, a stirring barrel and a discharging barrel, and the moving device comprises a second rotating shaft, a first rolling wheel, a driving wheel, a driven wheel and a track. A stirring rod arranged on a first rotating shaft stirs feed in a stirring barrel, blockage caused by the fact that the feed is bonded into blocks is prevented, a discharging barrel is driven to rotate, the feed is fed from a discharging opening formed in the side wall of the discharging barrel through centrifugal force, and feeding in different directions can be achieved; meanwhile, a driving wheel drives a driven wheel through a belt, so that a second rotating shaft is driven to rotate, a first rolling wheel rolls, and the moving effect of the whole device is achieved. The net cage is convenient to operate, uniform in feeding and flexible to move.

The invention provides a sex-linked molecular marker-assisted all-male silurus asotus breeding method, which belongs to the technical field of silurus asotus sex breeding, and comprises the following steps: step 1, screening a molecular marker SSR-Y1 linked with silurus asotus Y chromosome, and the gene base sequence of the molecular marker SSR-Y1 is as shown in SEQ ID NO: 1; 2, designing a specific primer pair SSR-Y1: F: 5 '-ATGTCGATCCGAGACCT-3' and a specific primer pair SSR-Y1: R: 5 '-TGCAGATCGTCGATAGG-3', and carrying out amplification on the specific primer pair SSR-Y1: F: 5 '-ATGTCGATCGAGACCT-3'; 3, extracting the genome DNA of an individual to be detected, and amplifying the SSR-Y1 marker through PCR (Polymerase Chain Reaction); 4, judging the gender according to an amplification product, wherein male individuals show 350bp bands, and female individuals do not have bands; and 5, selecting a male parent with the genetic sex of XY, and hybridizing the male parent with the common female XX to obtain an all-male offspring XY. According to the invention, the SSR-Y1 marker is adopted, and the SSR-Y1 marker has the advantages of strong specificity, high accuracy, no consumption of a large amount of manpower and low cost; the genetic sex of the catfish is rapidly identified through PCR amplification, the production process of unisexual fish fries of the catfish is simplified by means of a molecular marker-assisted breeding technology, the breeding speed is increased, and the cost is saved.

The invention discloses a method for improving the survival rate of yellow catfish transportation, comprising the steps of: (1) selecting healthy yellow catfish fry to be put into aquaculture water tank, water filling in the water tank, then adding composite polysaccharide nanoparticles and composite probiotics, and the yellow catfish fry is temporarily kept in the tank for 1 3 days without food; (2) the yellow catfish fry temporarily kept without food by water and step (1) are packed in aquatic product special oxygen bag, immediately oxygenating and sealing and ensuring that oxygen content is 6 7mg / L, and the bag is put into the carton of inner foam for transportation; the composite polysaccharide nanoparticles include chitosan, astragalus polysaccharide, oligoxylose, roxburghii polysaccharide and wolfberry polysaccharide and are made. The composite polysaccharide nanoparticles of the present invention are conducive to the absorption of polysaccharide and act on tissue, increase water solubility, dispersibility, and the absorption of fish body thereto, effectively avoid the side effect of polysaccharide accumulation and pollute water body, reduce the time and economic cost added to the feed, thus realize the relief of the fish stress under transportation stress.

The utility model discloses a leiocassis longirostris fry rearing pond algae removing device which comprises a rearing pond body, a first scraping plate is arranged in an inner cavity of the rearing pond body, a first sliding rod is fixedly connected to the top of the rearing pond body, a first threaded rod is rotationally connected to the top of the rearing pond body, and a second sliding rod is fixedly connected to the top of the rearing pond body. Two symmetrical sliding blocks are arranged at the top of the cultivation pond body, and one sliding block is in sliding connection with the first sliding rod. When pond algae appear in the inner cavity of the cultivation pond body, the first motor is started to enable the first scraper to scrape the pond algae on the surface of pond water to the left side of the second scraper, the second motor is started to enable the second scraper to move leftwards, and when the pond algae are scraped to the top of the collecting basket, the pond algae can be lifted out of the inner cavity of the cultivation pond body to be collected. Therefore, the purposes of cleaning the algae in the culture pond through the cleaning device, avoiding oxygen deficit of the water body due to growth of the algae in the pond and preventing large-area death of the fries due to oxygen deficit can be achieved.

The invention provides a channel catfish activeness testing system and method, and belongs to the technical field of aquaculture. Comprising a monitoring unit, a data processing unit, a control and display unit and a data storage and transmission unit, the data processing unit comprehensively processes data monitored by the monitoring unit, and the control and display unit controls and displays the monitoring unit and the data processing unit. And the data storage and transmission unit is used for storing data or analysis results of the monitoring unit and the data processing unit and transmitting the data or analysis results to a management platform or a mobile terminal. According to the method, data are collected through various sensors, the subjectivity and limitation of traditional manual observation are overcome, the activeness of channel catfish can be evaluated more accurately, and a reliable basis is provided for evaluating the health condition and the growth environment of fish.

The present invention provides a specific molecular marker, primer and kit for rapid identification of southern catfish, catfish and their hybrids. The primer sequences are shown in SEQ ID: No.1 and SEQ ID: No.2. The specific molecular marker is located in the gene gsdf The molecular marker for the southern catfish is 595 bp long, with the nucleotide sequence shown in SEQ ID No. 3. The molecular marker for the catfish is 522 bp long, with the nucleotide sequence shown in SEQ ID No. 4. The identification process of the present invention requires only the clipping of a small number of fin rays, extraction of genomic DNA, PCR amplification using the primers, and subsequent agarose gel electrophoresis. It also has high accuracy and is not restricted by fish growth, allowing sampling and identification at various stages of breeding. It can be widely used in scientific research and promotion institutions, seedling breeding farms, and aquaculture businesses. It can be used to identify seedlings of the three catfish species (southern catfish, catfish, and their hybrids) and to establish germplasm standards for the southern catfish, catfish, and their hybrids.

The present invention discloses a system and method for monitoring the dynamics of yellow catfish populations in yellow catfish (Psoralea vachelli), which specifically relate to the technical field of fishery resource monitoring. The system and method are used to solve the problems of inaccurate juvenile survival rate assessment and poor monitoring timeliness in benthic hidden environments in the prior art. The system deploys a benthic penetrating sonar array to synchronously collect water body reflection signals and benthic environment samples, and uses environmental DNA enrichment technology to extract juvenile-specific gene fragments to generate concentration distribution data. The system uses an acoustic reflection signal and body length correlation model to screen juvenile distribution data, and locates activity hotspots through spatiotemporal overlap analysis. A directional acoustic wave excitation device is deployed to obtain escape trajectory data, and the survival rate is calculated using a density attenuation model. Finally, multi-source data and terrain features are integrated to generate real-time population dynamics monitoring results, thereby achieving high-precision, non-invasive monitoring of juvenile fish populations in hidden environments.

This invention discloses a device and method for de-adheding fertilized eggs of the spiny loach. The device includes an incubator with a first guide rail and a second guide rail above it. One end of the second guide rail is connected to the first guide rail via a fixing rod. A first frame is movably disposed between the first guide rails, and a temporary storage box is rotatably mounted on the first frame. A first vertical block is connected to the outer wall of the temporary storage box. A second frame is movably disposed between the second guide rails, and a second vertical block is mounted on the second frame. A first linkage mechanism is provided between the second vertical block and the first vertical block to lock or unlock the connection between the second and first vertical blocks. A second linkage mechanism is also provided between the second frame and the fixing rod to lock or unlock the connection between the second frame and the fixing rod. This device for de-adheding fertilized eggs can regulate the de-adhesion and pouring of fertilized eggs, and evenly distribute the fertilized eggs into the incubator.

The present invention discloses an application of a SNP molecular marker and a detection reagent thereof in the breeding of disease-resistant fish varieties. In order to promote the breeding of Su's round-bellied catfish varieties resistant to bacterial septicemia, the present invention provides a SNP molecular marker, the SNP site of which is located at position 16886517 of the Su's round-bellied catfish reference genome CM018562.1, and the polymorphism is A or T. The SNP molecular marker of the present invention is related to the bacterial septicemia resistance trait of Su's round-bellied catfish and can be used in molecular marker-assisted breeding of Su's round-bellied catfish-related resistant varieties. The present invention also provides a detection reagent for the SNP molecular marker. By using the provided detection reagent to detect the SNP molecular marker, the bacterial septicemia resistance trait of Su's round-bellied catfish can be identified, and Su's round-bellied catfish individuals with relatively stronger resistance can be screened to obtain Su's round-bellied catfish individuals with relatively stronger resistance, thereby accelerating the breeding of Su's round-bellied catfish varieties resistant to bacterial septicemia.

The invention provides an ultralow-temperature preservation solution for sperms of mystus nemurus as well as a preparation method and application of the ultralow-temperature preservation solution, and belongs to the technical field of artificial breeding of mystus nemurus. The cryopreservation solution for sperms of mystus nemurus comprises a diluent and acetamide, wherein the final concentration of the acetamide is 80-150g / L; the diluent comprises the following components: sodium chloride, calcium chloride, magnesium sulfate, potassium chloride, glucose, astragalus polysaccharide and taurine. The ultra-low temperature preserving fluid effectively keeps the original pH of semen, maintains the osmotic pressure of the semen and inhibits sperm activation, the addition of acetamide reduces the freezing point of cytoplasm and prevents the formation of ice crystals, and the addition of astragalus polysaccharide and taurine improves the mitochondrial function of sperm and enhances the toughness of cell membranes. The survival rate and the functionality of the frozen sperms are effectively improved.

The utility model relates to the technical field of kitchen utensils, in particular to a fish skin black skin removing device which comprises an inner cylinder, an outer cylinder, a handle, a head fixator and a tail fixator. The inner cylinder is elastically connected with the outer cylinder, and the head fixator and the tail fixator are rotationally connected with the outer cylinder; the head fixator is used for clamping the head of a fish, the tail fixator is used for clamping the tail of the fish, the handle is arranged on the side where the head fixator is located, and the handle is fixedly connected with the head fixator; a skin scraper is arranged on the inner surface of the inner cylinder, and the inner cylinder is formed by rolling up an elastic plate. Fish is placed in the inner cylinder, the head fixing device and the tail fixing device are used for fixing the fish body, the handle is rotated, mucus and black skin on the fish body are scraped off by the skin scraping device, the mucus and black skin treatment efficiency of the device is higher, the treatment effect is better, and the effect is particularly remarkable under the condition of large-scale treatment of the leiocassis longirostris.

The invention discloses a method and system for monitoring a cowfish through unmanned aerial vehicle cruising and real-time video, and the method comprises the steps: S1, carrying out the cruising monitoring of the unmanned aerial vehicle above a target water area, shooting a video image, and selecting a picture frame including the cowfish in the video image; s2, marking the picture frames including the cowfish to obtain a cowfish image data set; pre-processing the images in the globefish image data set; s3, training the deep learning model by using the Jiangguinea pig image data set; s4, by means of the deep learning model constructed through training, real-time detection is carried out on the real-time transmitted unmanned aerial vehicle monitoring video, the cowfishes are marked in the detected video, and the number of the cowfishes is counted; and S5, statistics is carried out by using the appearing time of the video frame image of the dolphin in the video to obtain the appearing time of the dolphin and the total time when the dolphin is exposed out of the water surface. According to the method, the problem of low accuracy of catfish identification is solved, and the method has the characteristics of high robustness, strong generalization ability and high accuracy.

The utility model discloses a cultivation isolation device for cultivating yellow mulberry fish, which comprises an isolation bin, the isolation bin is divided into two parts, namely a cultivation bin and a recovery bin, a waterproof partition plate is arranged at the joint of the cultivation bin and the recovery bin, a fixing block is mounted at the upper end of the waterproof partition plate, a supporting column is mounted at the lower end of the fixing block, and the waterproof partition plate is connected with the supporting column. An electric telescopic rod is installed in the inner wall of the supporting column, a connecting column is arranged at the top end of the electric telescopic rod, and a filter plate is fixedly installed at the bottom end of the connecting column. The filter plate capable of ascending and descending is arranged in the breeding bin, the filter plate is lifted from the bottom end of the breeding bin in the harvest season, penetrates through sludge to drive and lift yellow mulberry fish in the breeding bin to a certain position, and then a breeder washes the yellow mulberry fish from one side through a water pipe to push the yellow mulberry fish. And the catfish can slide into the recycling bin on one side to be recycled and collected, so that the catfish catching process is simplified, and the catfish can be more conveniently caught after being cultivated and formed.

The present invention belongs to the field of Yangtze sturgeon breeding, and discloses a method and application of natural breeding of Yangtze sturgeon in open waters. The present invention, for the first time, practices the transformation and reconstruction of Yangtze sturgeon spawning grounds from the perspective of engineering implementation, proves the feasibility of repairing and transforming Yangtze sturgeon spawning grounds, realizes the natural breeding of Yangtze sturgeon in open waters, is a breakthrough research result of Yangtze sturgeon, and lays a solid foundation for the restoration of Yangtze sturgeon habitats.

The invention provides a family breeding method for large-scale barbel, comprising the following steps: one, selecting parents; two, disinfecting; three, cultivating parents; four, artificially inducing spawning; five, artificial insemination; six, cultivating fry; and seven, late family cultivation. The invention introduces high-quality exogenous genes of wild large-scale barbel, which can effectively improve the body color differentiation, frequent diseases, slow growth, decreased fecundity and other phenomena of existing cultured large-scale barbel, and is helpful to breed new strains with excellent growth traits such as fast growth. The invention significantly improves the fertilization rate, hatching rate and fry survival rate of large-scale barbel by optimizing conditions such as cultivating parent fish, types and concentration ratios of oxytocin, injection methods and feed types.

The present application relates to long-nosed grass carp breeding technical field, especially green pollution-free long-nossed grass carp breeding method, aiming at the single purification effect of the breeding water in the green breeding process of long-nosed grass carp in the prior art, the present application provides the following scheme, which comprises the following steps: S1, establishing a breeding farm, building suitable breeding ponds according to the breeding scale, and laying bricks and stones or cement hardening treatment on the bottom and side of the breeding pond; S2, establishing a water purification device, installing a certain number of water purification devices in the breeding pond, and regularly starting to purify the breeding water in the breeding pond; S3, prevention and treatment of diseases and insect pests, before putting fish fry, the bottom is comprehensively sprayed with quicklime, the present application not only can conveniently purify the breeding water in the breeding pond, but also can effectively improve the oxygen content in the breeding water in the filtering process, and can also avoid the winding of the water tank on the suction pipe.

This utility model discloses a circulating water purification device for the cultivation of *Cetacea purpurea*, relating to the field of *Cetacea purpurea* cultivation technology. It includes a cultivation box with a flow guide frame at the top and a water storage tank at the bottom. The water storage tank contains a filter assembly, and its inner wall is connected to a vertically arranged water pipe. The beneficial effects of this utility model are: the cultivation box, composed of a frame and a glass plate, allows observation of the *Cetacea purpurea* inside the box from the outside; the flow guide frame, with its spiral structure, utilizes gravity to guide the water flow naturally, reducing power consumption and extending the water flow path, thus improving purification and oxygenation efficiency; the filter assembly purifies the water in the cultivation box; and the ramp and partitions simulate the flow of a natural river through a circulating water system.

1. The name of the designed product: lighter case (sail tail fish). 2. The use of the designed product: used for containing lighters. 3. The design points of the designed product: in shape. 4. The picture or photo that best shows the design points: perspective view 1.

The invention provides a method for food conversion of silurus meridionalis fries, and belongs to the technical field of food conversion of silurus meridionalis fries. According to the feed conversion method for the silurus meridionalis offspring seeds, in the offspring seed feed conversion stage, the tubificidae is firstly fed to open mouths, then the pork liver is fed for offspring seed breeding, then the pork liver and silurus meridionalis offspring seed powder in different proportions are used for preparing feed conversion feed for feeding, and the offspring seeds smoothly eat artificial compound feed through 25-36-day step-by-step feed conversion, so that the yield of the offspring seeds is improved. The specification of the larvae grows from the initial 0.5-1.7 g / larvae to 7-12 g / larvae, the larvae are fed, and the larvae feeding conversion survival rate can reach 89.5% or above. Meanwhile, screening and fish separation are conducted once every 6-9 days in the food conversion breeding process, the fries are separated into different specifications to be bred, and the situation that the fries kill one another due to large specification difference is effectively prevented.

The invention relates to the technical field of fishery breeding and discloses an automatic feeding device for leiocassis longirostris breeding, the automatic feeding device comprises a base, a top plate arranged above the base and a feeding box arranged between the base and the top plate, a plurality of feeding holes are formed in the two sides of the feeding box, and discharging pipes are fixedly inserted into the feeding holes; a mounting plate is slidably connected to the interior of the feeding box, closing plates sliding in the feeding box are fixed to the two sides of the mounting plate, the closing plates are used for closing feeding holes of the feeding box, a push plate sliding in the feeding box is arranged below the mounting plate, and a dredging assembly is further arranged on the outer side of the feeding box. According to the leiocassis longirostris feeding device, the fed leiocassis longirostris can be fed with feed quickly and uniformly, the splashing range of the fed feed can be regulated and controlled, the feeding efficiency is high, the working performance is high, the splashing amount of the feed can be controlled according to the growth state of the leiocassis longirostris, and use is convenient.

The invention belongs to the field of animal molecular biology, and relates to a leiocassis crassilabris enhancement and release effect evaluation method which comprises the following steps: 1) storing a DNA parent of an artificial propagation parent of leiocassis crassilabris; 2) releasing the leiocassis crassilabris artificial propagation offspring; (3) catching field groups of leiocassis crassilabris; 4) extracting DNA of caught field groups of leiocassis crassilabris, and catching; (5) respectively carrying out PCR amplification on the DNA parent obtained in the step (1) and the DNA fishing obtained in the step (4) by utilizing the primer group for identifying the leiocassis crassilabris, and carrying out polyacrylamide gel electrophoresis on PCR products; and 6) analyzing the parent-child relationship between the leiocassis crassilabris parents and the leiocassis crassilabris caught in the field according to the result of the step 5) to obtain the number of the leiocassis crassilabris parents in the caught leiocassis crassilabris, and evaluating the releasing effect according to the number. The invention provides the leiocassis crassilabris enhancement releasing effect evaluation method which can accurately evaluate the leiocassis crassilabris releasing effect and can improve the efficiency of the releasing effect.

This utility model discloses a high-efficiency and energy-saving ecological breeding pond for *Cetacea purpurea*, relating to the field of breeding pond technology. It includes a frame, with a filter box installed at the top of the frame. The inner wall of the frame is respectively provided with a first inclined plate and a sieve frame. Two sets of water conveying components are provided on the front of the filter box, and a funnel is installed at the bottom of the filter box. A fixing frame is fixed to the inner wall of the frame. The beneficial effects of this utility model are: the filter box can store filter material; when the water conveying components deliver breeding water to the filter box, the water can be filtered through the filter material inside; the first inclined plate can simulate the flow of a natural river through a circulating water system; and the sieve frame can filter the breeding water through the openings in its inner wall, while simultaneously collecting residual feed, feces, and harmful substances in the water.

The utility model discloses a smelly mandarin fish wooden barrel pickling and barreling device which comprises a device main body, a control panel is arranged outside the device main body, a feeding conveying belt is arranged at the top of the device main body, a wooden barrel body is arranged in the device main body, and a material preparation barrel is further arranged in the device main body. The device comprises a material preparation barrel, a material guide barrel is fixedly connected to the bottom of the material preparation barrel, a material pickling bin is fixedly connected to one side of the outer portion of the material preparation barrel, a material spreading box is fixedly connected to the bottom of the material pickling bin, and material scattering holes are formed in the bottom face of the material spreading box. The outer thorns among the mandarin fishes are mutually interspersed, so that the benefit that the integrity of the salted smelly mandarin fishes cannot be ensured is solved. The smelly mandarin fish stacking machine has the technical effects that each smelly mandarin fish can be ensured to keep certain integrity in a manner of stacking the smelly mandarin fish layer by layer by operating the machine.