66 results about "Catfish" patented technology

The invention discloses a treatment method for fermenting smelly mandarin fish by coupling gradient salt control and directional inoculation, which comprises the following steps of: pretreating the smelly mandarin fish, putting the smelly mandarin fish into ozone-containing water for bacteria reduction treatment, then putting the smelly mandarin fish into a salt solution for pickling, finally inoculating bacteria liquid into the pickled smelly mandarin fish for fermentation treatment, cleaning, draining, packaging and quickly freezing after fermentation is finished, and thus obtaining the smelly mandarin fish. According to the method, the fermentation production process of the smelly mandarin fish is adjusted and standardized, the microorganism content of the slaughtered smelly mandarin fish is reduced through pretreatment, then the bacteria-reduced smelly mandarin fish is pickled with a low-concentration sodium chloride aqueous solution, meanwhile, the screened strains (psychrobacter halophilus and lactobacillus plantarum) are inoculated, and compared with traditional fermentation, the method is easy to operate, low in cost and high in yield. The product quality is stable, the defective rate is low, the labor cost is saved, the speed is high, the volatile basic nitrogen of the obtained smelly mandarin fish is less than or equal to 30mg / 100g, and the peroxide value (in terms of fat) is less than or equal to 2.5 g / 100g.

The invention discloses a low-stress bleeding processing method for channel catfish. The method comprises the following steps: an acid-base control step, in which the channel catfish is placed in water containing sodium citrate to control the acid-base in the body, thereby increasing the alkali reserve in the body of the fish; a combined light anesthesia step, in which the channel catfish is lightly anesthetized through the combined action of low temperature and a chemical anesthetic, so that the fish exhibits a state in which the gill cover of the fish opens and closes weakly and slowly, and the fish body rolls over and loses balance; a fish body puncture step, in which the blood circulation of the channel catfish is destroyed by a manual or mechanical method, thereby creating a bleeding wound on the fish body; a metabolic bleeding step, in which the fish body is placed in a bleeding tank that has been aerated and oxygenated in advance to saturated dissolved oxygen, for bleeding, while maintaining a certain dissolved oxygen concentration; and an ultrasonic blood purification step, in which the fish body is placed in a bleeding tank equipped with an ultrasonic device for bleeding, thereby accelerating the discharge of residual blood in the body.

The invention discloses a channel catfish gill bloodletting point self-adaptive positioning mechanism which comprises a horizontal sliding block seat in transmission connection with a driving component and driven by the driving component to move front and back. The tool apron is connected with the horizontal sliding block base in a left-right sliding mode and can conduct self-adaptive movable adjustment relative to the horizontal sliding block base, the sliding direction of the tool apron is perpendicular to the sliding direction of the horizontal sliding block base, spring bases are fixedly connected to the left side and the right side of the horizontal sliding block base correspondingly, and spring sets are connected to the left side and the right side of the tool apron correspondingly. The left side / right side of the tool apron is elastically connected with the spring seat on the left side / right side through a spring group; the cutter module is used for puncturing a fish body and can be adjusted in a self-adaptive mode, and the cutter module is rotatably, movably and adjustably connected with the cutter holder. By adopting the scheme of the invention, the problems of gill bloodletting point identification and positioning in the prior art can be solved.

The invention discloses an SSR (Simple Sequence Repeat) primer composition for evaluating genetic diversity of channel catfish breeding population and an application method of the SSR primer composition, and belongs to the technical field of fish genetic breeding. The method comprises the following steps: modifying 5'ends of forward primers of the primers by using FAM fluorophores respectively, amplifying genome DNA of each breeding group by using the 10 pairs of microsatellite site primers respectively, analyzing amplification products, reading genotypes, analyzing parameters, and determining the genotype of the breeding group. The analysis result is used for evaluating the population polymorphism, the variation condition of the population genetic structure and the genetic differentiation degree among the populations. By applying the evaluation method disclosed by the invention, the population genetic diversity and genetic structure of continuous breeding generations of channel catfish can be systematically evaluated. The SSR primer and the genetic diversity evaluation method can be used for genetic improvement and new variety breeding of channel catfish.

The utility model provides a sewage suction device for a land-based barrel culture pond of mastacembelus armatus, which comprises a culture pond, a first sewage suction pipe, a second sewage suction pipe, a collecting box, a third sewage suction pipe and a filter, the bottom of the culture pond is in an inverted cone shape, one end of the first sewage suction pipe is connected with the filter, and the other end of the first sewage suction pipe extends to the lowest point of the bottom of the culture pond; one end of the second sewage suction pipe is connected with the filter, the other end of the second sewage suction pipe is connected with an inner cavity of the collecting box, and the bottom of the collecting box is 20-30 cm lower than the water level of the culture pond; one end of the third sewage suction pipe is connected with the bottom of the collection box. As the sealing cover for sealing is arranged on the collecting box, negative pressure can be formed after water in the collecting box is pumped by the water pump, water flows into the collecting box in an accelerated mode through the third sewage suction pipe, the suction force of the third sewage suction pipe is improved, the higher sewage suction capacity is obtained, sewage at the bottom of a culture pond and diseased and dead fishes can be better sucked into the collecting box, and therefore the quality of the culture pond is improved. The water quality influence caused by rotting of diseased and dead fishes is avoided.

The invention provides a sex-linked molecular marker-assisted all-male silurus asotus breeding method, which belongs to the technical field of silurus asotus sex breeding, and comprises the following steps: step 1, screening a molecular marker SSR-Y1 linked with silurus asotus Y chromosome, and the gene base sequence of the molecular marker SSR-Y1 is as shown in SEQ ID NO: 1; 2, designing a specific primer pair SSR-Y1: F: 5 '-ATGTCGATCCGAGACCT-3' and a specific primer pair SSR-Y1: R: 5 '-TGCAGATCGTCGATAGG-3', and carrying out amplification on the specific primer pair SSR-Y1: F: 5 '-ATGTCGATCGAGACCT-3'; 3, extracting the genome DNA of an individual to be detected, and amplifying the SSR-Y1 marker through PCR (Polymerase Chain Reaction); 4, judging the gender according to an amplification product, wherein male individuals show 350bp bands, and female individuals do not have bands; and 5, selecting a male parent with the genetic sex of XY, and hybridizing the male parent with the common female XX to obtain an all-male offspring XY. According to the invention, the SSR-Y1 marker is adopted, and the SSR-Y1 marker has the advantages of strong specificity, high accuracy, no consumption of a large amount of manpower and low cost; the genetic sex of the catfish is rapidly identified through PCR amplification, the production process of unisexual fish fries of the catfish is simplified by means of a molecular marker-assisted breeding technology, the breeding speed is increased, and the cost is saved.

The invention discloses a method for improving the survival rate of yellow catfish transportation, comprising the steps of: (1) selecting healthy yellow catfish fry to be put into aquaculture water tank, water filling in the water tank, then adding composite polysaccharide nanoparticles and composite probiotics, and the yellow catfish fry is temporarily kept in the tank for 1 3 days without food; (2) the yellow catfish fry temporarily kept without food by water and step (1) are packed in aquatic product special oxygen bag, immediately oxygenating and sealing and ensuring that oxygen content is 6 7mg / L, and the bag is put into the carton of inner foam for transportation; the composite polysaccharide nanoparticles include chitosan, astragalus polysaccharide, oligoxylose, roxburghii polysaccharide and wolfberry polysaccharide and are made. The composite polysaccharide nanoparticles of the present invention are conducive to the absorption of polysaccharide and act on tissue, increase water solubility, dispersibility, and the absorption of fish body thereto, effectively avoid the side effect of polysaccharide accumulation and pollute water body, reduce the time and economic cost added to the feed, thus realize the relief of the fish stress under transportation stress.

The present invention provides a specific molecular marker, primer and kit for rapid identification of southern catfish, catfish and their hybrids. The primer sequences are shown in SEQ ID: No.1 and SEQ ID: No.2. The specific molecular marker is located in the gene gsdf The molecular marker for the southern catfish is 595 bp long, with the nucleotide sequence shown in SEQ ID No. 3. The molecular marker for the catfish is 522 bp long, with the nucleotide sequence shown in SEQ ID No. 4. The identification process of the present invention requires only the clipping of a small number of fin rays, extraction of genomic DNA, PCR amplification using the primers, and subsequent agarose gel electrophoresis. It also has high accuracy and is not restricted by fish growth, allowing sampling and identification at various stages of breeding. It can be widely used in scientific research and promotion institutions, seedling breeding farms, and aquaculture businesses. It can be used to identify seedlings of the three catfish species (southern catfish, catfish, and their hybrids) and to establish germplasm standards for the southern catfish, catfish, and their hybrids.

The present invention discloses a system and method for monitoring the dynamics of yellow catfish populations in yellow catfish (Psoralea vachelli), which specifically relate to the technical field of fishery resource monitoring. The system and method are used to solve the problems of inaccurate juvenile survival rate assessment and poor monitoring timeliness in benthic hidden environments in the prior art. The system deploys a benthic penetrating sonar array to synchronously collect water body reflection signals and benthic environment samples, and uses environmental DNA enrichment technology to extract juvenile-specific gene fragments to generate concentration distribution data. The system uses an acoustic reflection signal and body length correlation model to screen juvenile distribution data, and locates activity hotspots through spatiotemporal overlap analysis. A directional acoustic wave excitation device is deployed to obtain escape trajectory data, and the survival rate is calculated using a density attenuation model. Finally, multi-source data and terrain features are integrated to generate real-time population dynamics monitoring results, thereby achieving high-precision, non-invasive monitoring of juvenile fish populations in hidden environments.

This invention discloses a device and method for de-adheding fertilized eggs of the spiny loach. The device includes an incubator with a first guide rail and a second guide rail above it. One end of the second guide rail is connected to the first guide rail via a fixing rod. A first frame is movably disposed between the first guide rails, and a temporary storage box is rotatably mounted on the first frame. A first vertical block is connected to the outer wall of the temporary storage box. A second frame is movably disposed between the second guide rails, and a second vertical block is mounted on the second frame. A first linkage mechanism is provided between the second vertical block and the first vertical block to lock or unlock the connection between the second and first vertical blocks. A second linkage mechanism is also provided between the second frame and the fixing rod to lock or unlock the connection between the second frame and the fixing rod. This device for de-adheding fertilized eggs can regulate the de-adhesion and pouring of fertilized eggs, and evenly distribute the fertilized eggs into the incubator.

The present invention discloses an application of a SNP molecular marker and a detection reagent thereof in the breeding of disease-resistant fish varieties. In order to promote the breeding of Su's round-bellied catfish varieties resistant to bacterial septicemia, the present invention provides a SNP molecular marker, the SNP site of which is located at position 16886517 of the Su's round-bellied catfish reference genome CM018562.1, and the polymorphism is A or T. The SNP molecular marker of the present invention is related to the bacterial septicemia resistance trait of Su's round-bellied catfish and can be used in molecular marker-assisted breeding of Su's round-bellied catfish-related resistant varieties. The present invention also provides a detection reagent for the SNP molecular marker. By using the provided detection reagent to detect the SNP molecular marker, the bacterial septicemia resistance trait of Su's round-bellied catfish can be identified, and Su's round-bellied catfish individuals with relatively stronger resistance can be screened to obtain Su's round-bellied catfish individuals with relatively stronger resistance, thereby accelerating the breeding of Su's round-bellied catfish varieties resistant to bacterial septicemia.

The invention provides an ultralow-temperature preservation solution for sperms of mystus nemurus as well as a preparation method and application of the ultralow-temperature preservation solution, and belongs to the technical field of artificial breeding of mystus nemurus. The cryopreservation solution for sperms of mystus nemurus comprises a diluent and acetamide, wherein the final concentration of the acetamide is 80-150g / L; the diluent comprises the following components: sodium chloride, calcium chloride, magnesium sulfate, potassium chloride, glucose, astragalus polysaccharide and taurine. The ultra-low temperature preserving fluid effectively keeps the original pH of semen, maintains the osmotic pressure of the semen and inhibits sperm activation, the addition of acetamide reduces the freezing point of cytoplasm and prevents the formation of ice crystals, and the addition of astragalus polysaccharide and taurine improves the mitochondrial function of sperm and enhances the toughness of cell membranes. The survival rate and the functionality of the frozen sperms are effectively improved.

The utility model relates to the technical field of kitchen utensils, in particular to a fish skin black skin removing device which comprises an inner cylinder, an outer cylinder, a handle, a head fixator and a tail fixator. The inner cylinder is elastically connected with the outer cylinder, and the head fixator and the tail fixator are rotationally connected with the outer cylinder; the head fixator is used for clamping the head of a fish, the tail fixator is used for clamping the tail of the fish, the handle is arranged on the side where the head fixator is located, and the handle is fixedly connected with the head fixator; a skin scraper is arranged on the inner surface of the inner cylinder, and the inner cylinder is formed by rolling up an elastic plate. Fish is placed in the inner cylinder, the head fixing device and the tail fixing device are used for fixing the fish body, the handle is rotated, mucus and black skin on the fish body are scraped off by the skin scraping device, the mucus and black skin treatment efficiency of the device is higher, the treatment effect is better, and the effect is particularly remarkable under the condition of large-scale treatment of the leiocassis longirostris.

The utility model discloses a cultivation isolation device for cultivating yellow mulberry fish, which comprises an isolation bin, the isolation bin is divided into two parts, namely a cultivation bin and a recovery bin, a waterproof partition plate is arranged at the joint of the cultivation bin and the recovery bin, a fixing block is mounted at the upper end of the waterproof partition plate, a supporting column is mounted at the lower end of the fixing block, and the waterproof partition plate is connected with the supporting column. An electric telescopic rod is installed in the inner wall of the supporting column, a connecting column is arranged at the top end of the electric telescopic rod, and a filter plate is fixedly installed at the bottom end of the connecting column. The filter plate capable of ascending and descending is arranged in the breeding bin, the filter plate is lifted from the bottom end of the breeding bin in the harvest season, penetrates through sludge to drive and lift yellow mulberry fish in the breeding bin to a certain position, and then a breeder washes the yellow mulberry fish from one side through a water pipe to push the yellow mulberry fish. And the catfish can slide into the recycling bin on one side to be recycled and collected, so that the catfish catching process is simplified, and the catfish can be more conveniently caught after being cultivated and formed.

The present application relates to long-nosed grass carp breeding technical field, especially green pollution-free long-nossed grass carp breeding method, aiming at the single purification effect of the breeding water in the green breeding process of long-nosed grass carp in the prior art, the present application provides the following scheme, which comprises the following steps: S1, establishing a breeding farm, building suitable breeding ponds according to the breeding scale, and laying bricks and stones or cement hardening treatment on the bottom and side of the breeding pond; S2, establishing a water purification device, installing a certain number of water purification devices in the breeding pond, and regularly starting to purify the breeding water in the breeding pond; S3, prevention and treatment of diseases and insect pests, before putting fish fry, the bottom is comprehensively sprayed with quicklime, the present application not only can conveniently purify the breeding water in the breeding pond, but also can effectively improve the oxygen content in the breeding water in the filtering process, and can also avoid the winding of the water tank on the suction pipe.

This utility model discloses a circulating water purification device for the cultivation of *Cetacea purpurea*, relating to the field of *Cetacea purpurea* cultivation technology. It includes a cultivation box with a flow guide frame at the top and a water storage tank at the bottom. The water storage tank contains a filter assembly, and its inner wall is connected to a vertically arranged water pipe. The beneficial effects of this utility model are: the cultivation box, composed of a frame and a glass plate, allows observation of the *Cetacea purpurea* inside the box from the outside; the flow guide frame, with its spiral structure, utilizes gravity to guide the water flow naturally, reducing power consumption and extending the water flow path, thus improving purification and oxygenation efficiency; the filter assembly purifies the water in the cultivation box; and the ramp and partitions simulate the flow of a natural river through a circulating water system.

1. The name of the designed product: lighter case (sail tail fish). 2. The use of the designed product: used for containing lighters. 3. The design points of the designed product: in shape. 4. The picture or photo that best shows the design points: perspective view 1.

The invention relates to the technical field of fishery breeding and discloses an automatic feeding device for leiocassis longirostris breeding, the automatic feeding device comprises a base, a top plate arranged above the base and a feeding box arranged between the base and the top plate, a plurality of feeding holes are formed in the two sides of the feeding box, and discharging pipes are fixedly inserted into the feeding holes; a mounting plate is slidably connected to the interior of the feeding box, closing plates sliding in the feeding box are fixed to the two sides of the mounting plate, the closing plates are used for closing feeding holes of the feeding box, a push plate sliding in the feeding box is arranged below the mounting plate, and a dredging assembly is further arranged on the outer side of the feeding box. According to the leiocassis longirostris feeding device, the fed leiocassis longirostris can be fed with feed quickly and uniformly, the splashing range of the fed feed can be regulated and controlled, the feeding efficiency is high, the working performance is high, the splashing amount of the feed can be controlled according to the growth state of the leiocassis longirostris, and use is convenient.

This utility model discloses a high-efficiency and energy-saving ecological breeding pond for *Cetacea purpurea*, relating to the field of breeding pond technology. It includes a frame, with a filter box installed at the top of the frame. The inner wall of the frame is respectively provided with a first inclined plate and a sieve frame. Two sets of water conveying components are provided on the front of the filter box, and a funnel is installed at the bottom of the filter box. A fixing frame is fixed to the inner wall of the frame. The beneficial effects of this utility model are: the filter box can store filter material; when the water conveying components deliver breeding water to the filter box, the water can be filtered through the filter material inside; the first inclined plate can simulate the flow of a natural river through a circulating water system; and the sieve frame can filter the breeding water through the openings in its inner wall, while simultaneously collecting residual feed, feces, and harmful substances in the water.

The utility model discloses a smelly mandarin fish wooden barrel pickling and barreling device which comprises a device main body, a control panel is arranged outside the device main body, a feeding conveying belt is arranged at the top of the device main body, a wooden barrel body is arranged in the device main body, and a material preparation barrel is further arranged in the device main body. The device comprises a material preparation barrel, a material guide barrel is fixedly connected to the bottom of the material preparation barrel, a material pickling bin is fixedly connected to one side of the outer portion of the material preparation barrel, a material spreading box is fixedly connected to the bottom of the material pickling bin, and material scattering holes are formed in the bottom face of the material spreading box. The outer thorns among the mandarin fishes are mutually interspersed, so that the benefit that the integrity of the salted smelly mandarin fishes cannot be ensured is solved. The smelly mandarin fish stacking machine has the technical effects that each smelly mandarin fish can be ensured to keep certain integrity in a manner of stacking the smelly mandarin fish layer by layer by operating the machine.

The present application relates to a kind of miniaturized eel and loach killing machine, belong to eel and loach killing equipment technical field.The miniaturized eel and loach killing machine, including killing box;The inside of the killing box is installed with upper material guide pipe and lower material guide pipe in upper and lower shape;Killing box between upper material guide pipe and lower material guide pipe is installed with cutting knife by cutting motor;The killing box is equipped with conveying mechanism;Conveying mechanism can drive eel and loach to move in the inside of upper material guide pipe and lower material guide pipe when working.The miniaturized eel and loach killing machine is compact in structure, clever in design, using a cutting knife can make the killing machine structure have the function of cutting belly and cutting back, not only save space, but also meet the use demand of multifunctional eel and loach killing, especially suitable for family and restaurant use.

The invention relates to the technical field of microbial fermentation, in particular to a method for preparing leiocassis longirostris oil through salting fermentation. The fish oil is produced by using the leiocassis longirostris viscera as the raw material, and the problem of high-value utilization of by-products such as the viscera in the leiocassis longirostris processing process can be solved. Results of the specific embodiment of the invention show that compared with a cooking method and a salting fermentation method, the fish oil can be rapidly produced without high-temperature heating, and the fish oil is brighter in color and better in quality, mainly has lower acid value, peroxide value, moisture and volatile matter content, higher iodine value and higher unsaturated fatty acid content, and has a wide market application prospect.

The utility model discloses a glyptosternon maculatum roe hatching device with an oxygenation device, and belongs to the technical field of roe hatching devices. The glyptosternon maculatum roe hatching device with the oxygenation device comprises a hatching barrel, an overflow groove is formed in the top end of the outer side of the hatching barrel, a screen is installed at the top end of the hatching barrel, flow guide mechanisms are arranged on the two sides of the interior of the hatching barrel, a fixing plate is installed on one side of the overflow groove, and a liquid inlet mechanism is installed on one side of the top end of the fixing plate. The output end of the liquid inlet mechanism is connected with the input end of the flow guide mechanism, an oxygenation mechanism is installed at the bottom end of the interior of the hatching barrel and comprises an air outlet disc, the bottom end of the air outlet disc is fixedly connected with the bottom end of the interior of the hatching barrel, a fan is installed on the other side of the top end of the fixing plate, and an air outlet pipe is installed at the output end of the fan; according to the oxygen increasing device for the hatching barrel, the oxygen increasing function can be effectively achieved, the oxygen increasing uniformity is high, and the high practical value is achieved.

The utility model discloses a water flow stirring device for ictalurus punctatus fry culture, a plurality of rotating shafts are rotatably mounted in a culture pond, one end of each rotating shaft extends to the outer side of the culture pond, a belt pulley is fixed on each rotating shaft, one end of one rotating shaft is connected with a driving motor, and a belt is arranged between any two adjacent belt pulleys. The driving motor can be started to be matched with the belt to drive the rotating shafts to rotate, the stirring assembly comprises mounting rings fixed to the peripheries of the rotating shafts, a plurality of stirring plates are fixed to the mounting rings, and when the rotating shafts rotate, the stirring plates can be driven to rotate, so that water flow in the culture pond is stirred, and the flowability of water is improved; the stirring assemblies on the multiple rotating shafts and any two adjacent rotating shafts are arranged at intervals, so that water at different positions in the culture pond can be stirred, and the water flow stirring effect is improved. A plurality of through holes are formed in each rotating shaft, water in the culture pond can flow through the through holes in the water flow stirring process, and the water flow stirring effect is further improved.

The present invention relates to the technical field of aquaculture methods, and specifically to a transportation method and a transportation system for farmed long-snouted catfish that improve the survival rate. The transportation method includes the entire process from pre-catching preparation to recuperation at the breeding ground, and the transportation system includes a pre-transportation treatment unit, a transportation unit, and a post-transportation temporary holding unit. By using the above-mentioned system and the above-mentioned method, the purpose of promoting the recovery of long-snouted catfish wounds and preventing and controlling diseases such as bacteria, molds, Ichthyophthirius and spores can be achieved, and it is suitable for daily long-distance transportation, parent transportation and cultivation of long-snouted catfish. This technical solution can solve the technical problem of the high mortality rate of long-snouted catfish after long-distance transportation in the prior art. This transportation method and system that improves the survival rate not only solves the challenges faced by long-snouted catfish during transportation, but also has important significance for promoting the healthy and stable development of the long-snouted catfish industry, and has ideal application and promotion prospects.

The invention relates to the technical field of dipsacus giganteus culture devices, and discloses a dipsacus giganteus culture device which comprises a culture pond, a mounting frame is mounted at the top of the culture pond, a release assembly is arranged on the inner wall of the mounting frame, a suction pump is fixedly assembled on the outer wall of the culture pond, and a flow guide assembly is mounted at the top of the suction pump. According to the device, when water in the inner cavity of the culture pond is sucked under the action of the suction pump, the water can be released into the inner cavity of the collecting box through the water discharging pipe, and in the process that the water flushes the water discharging pipe and enters the collecting box, the oxygen content in the water is increased, and the water is guided and released; and the release position of the connecting pipe can be adjusted according to the height of the water surface, so that severe mechanical impact on the water surface in the oxygenation release process is avoided, the influence of mechanical impact on gill filaments of the dipsacus dipsacus is further avoided, and the risk of stress reaction or disease of gill is reduced.

The invention provides a molecular marker combination for sex identification of glyptosternon maculatum, 53 SNP / INDEL sites closely linked with sex are screened, and the molecular marker combination comprises at least one of the following molecular markers. The unisexual breeding of the glyptosternon maculatum is facilitated, and the breeding process is accelerated.

The invention belongs to the technical field of fermentation processing of aquatic products, and particularly relates to a low-salt freshness-enhancing siniperca chuatsi processing method based on synergistic fermentation of functional complementary lactic acid bacteria. According to the method, endogenous lactic acid bacteria are separated from siniperca chuatsi, the salt tolerance and metabolic characteristics of the endogenous lactic acid bacteria are evaluated in the implementation process, and a lactic acid bacteria combination with complementary functions is preferably constructed. Through the synergistic fermentation effect, degradation of protein and lipid in fish meat is promoted, flavor substances beneficial to formation of delicate flavor are generated, and the delicate flavor and the overall quality of the smelly mandarin fish are improved under the condition that the use amount of salt is remarkably reduced. Compared with high-salt natural fermentation and low-salt natural fermentation, the smelly mandarin fish prepared by the method disclosed by the invention has higher umami response, better texture characteristics and lower pH value and water activity. The method disclosed by the invention is beneficial to improving the stability and safety of the product, and is suitable for standardized and large-scale production of the low-salt-odor mandarin fish.

The application discloses a kind of SSR and SNP molecular marker primer combination for genetic identification of southern catfish family and application thereof, belong to molecular biology field, the SSR molecular marker primer has 9 pairs, nucleotide sequence as shown in SEQ ID No.1-SEQ ID No.18;The SNP molecular marker primer has 6 pairs, nucleotide sequence as shown in SEQ ID No.19-SEQ ID No.30.The application uses 9 microsatellite sites and 6 southern catfish gene SNP mutations as molecular markers, which can distinguish different families of southern catfish, and construct the genetic pedigree of the breeding southern catfish family.The technology can lay a foundation for improving the germplasm purity of southern catfish breeding population and scientific breeding.The method of the application has the characteristics of simple operation and high accuracy.

According to the InDel specific molecular marker for identifying the genetic sex of the megalobrama amblycephala and the application, a primer pair is designed, sample genome DNA serves as a template, the genetic sex of the megalobrama amblycephala can be identified only through PCR and agarose gel electrophoresis, consumed time is short, damage to fish bodies is small, limitation of fish ages is avoided, the InDel specific molecular marker can be applied to the whole life cycle of the megalobrama amblycephala, and the application range of the InDel specific molecular marker is wide. The method is convenient and rapid, has higher accuracy, and is especially suitable for rapid sex determination of a large number of samples. The method provides basic data for further mining of the sex determining gene of the megalobrama amblycephala, and lays a foundation for sex control breeding of the megalobrama amblycephala.