47 results about "Catfish" patented technology

The invention discloses a treatment method for fermenting smelly mandarin fish by coupling gradient salt control and directional inoculation, which comprises the following steps of: pretreating the smelly mandarin fish, putting the smelly mandarin fish into ozone-containing water for bacteria reduction treatment, then putting the smelly mandarin fish into a salt solution for pickling, finally inoculating bacteria liquid into the pickled smelly mandarin fish for fermentation treatment, cleaning, draining, packaging and quickly freezing after fermentation is finished, and thus obtaining the smelly mandarin fish. According to the method, the fermentation production process of the smelly mandarin fish is adjusted and standardized, the microorganism content of the slaughtered smelly mandarin fish is reduced through pretreatment, then the bacteria-reduced smelly mandarin fish is pickled with a low-concentration sodium chloride aqueous solution, meanwhile, the screened strains (psychrobacter halophilus and lactobacillus plantarum) are inoculated, and compared with traditional fermentation, the method is easy to operate, low in cost and high in yield. The product quality is stable, the defective rate is low, the labor cost is saved, the speed is high, the volatile basic nitrogen of the obtained smelly mandarin fish is less than or equal to 30mg / 100g, and the peroxide value (in terms of fat) is less than or equal to 2.5 g / 100g.

The invention discloses a channel catfish gill bloodletting point self-adaptive positioning mechanism which comprises a horizontal sliding block seat in transmission connection with a driving component and driven by the driving component to move front and back. The tool apron is connected with the horizontal sliding block base in a left-right sliding mode and can conduct self-adaptive movable adjustment relative to the horizontal sliding block base, the sliding direction of the tool apron is perpendicular to the sliding direction of the horizontal sliding block base, spring bases are fixedly connected to the left side and the right side of the horizontal sliding block base correspondingly, and spring sets are connected to the left side and the right side of the tool apron correspondingly. The left side / right side of the tool apron is elastically connected with the spring seat on the left side / right side through a spring group; the cutter module is used for puncturing a fish body and can be adjusted in a self-adaptive mode, and the cutter module is rotatably, movably and adjustably connected with the cutter holder. By adopting the scheme of the invention, the problems of gill bloodletting point identification and positioning in the prior art can be solved.

The invention discloses an SSR (Simple Sequence Repeat) primer composition for evaluating genetic diversity of channel catfish breeding population and an application method of the SSR primer composition, and belongs to the technical field of fish genetic breeding. The method comprises the following steps: modifying 5'ends of forward primers of the primers by using FAM fluorophores respectively, amplifying genome DNA of each breeding group by using the 10 pairs of microsatellite site primers respectively, analyzing amplification products, reading genotypes, analyzing parameters, and determining the genotype of the breeding group. The analysis result is used for evaluating the population polymorphism, the variation condition of the population genetic structure and the genetic differentiation degree among the populations. By applying the evaluation method disclosed by the invention, the population genetic diversity and genetic structure of continuous breeding generations of channel catfish can be systematically evaluated. The SSR primer and the genetic diversity evaluation method can be used for genetic improvement and new variety breeding of channel catfish.

The utility model provides a sewage suction device for a land-based barrel culture pond of mastacembelus armatus, which comprises a culture pond, a first sewage suction pipe, a second sewage suction pipe, a collecting box, a third sewage suction pipe and a filter, the bottom of the culture pond is in an inverted cone shape, one end of the first sewage suction pipe is connected with the filter, and the other end of the first sewage suction pipe extends to the lowest point of the bottom of the culture pond; one end of the second sewage suction pipe is connected with the filter, the other end of the second sewage suction pipe is connected with an inner cavity of the collecting box, and the bottom of the collecting box is 20-30 cm lower than the water level of the culture pond; one end of the third sewage suction pipe is connected with the bottom of the collection box. As the sealing cover for sealing is arranged on the collecting box, negative pressure can be formed after water in the collecting box is pumped by the water pump, water flows into the collecting box in an accelerated mode through the third sewage suction pipe, the suction force of the third sewage suction pipe is improved, the higher sewage suction capacity is obtained, sewage at the bottom of a culture pond and diseased and dead fishes can be better sucked into the collecting box, and therefore the quality of the culture pond is improved. The water quality influence caused by rotting of diseased and dead fishes is avoided.

The invention provides a sex-linked molecular marker-assisted all-male silurus asotus breeding method, which belongs to the technical field of silurus asotus sex breeding, and comprises the following steps: step 1, screening a molecular marker SSR-Y1 linked with silurus asotus Y chromosome, and the gene base sequence of the molecular marker SSR-Y1 is as shown in SEQ ID NO: 1; 2, designing a specific primer pair SSR-Y1: F: 5 '-ATGTCGATCCGAGACCT-3' and a specific primer pair SSR-Y1: R: 5 '-TGCAGATCGTCGATAGG-3', and carrying out amplification on the specific primer pair SSR-Y1: F: 5 '-ATGTCGATCGAGACCT-3'; 3, extracting the genome DNA of an individual to be detected, and amplifying the SSR-Y1 marker through PCR (Polymerase Chain Reaction); 4, judging the gender according to an amplification product, wherein male individuals show 350bp bands, and female individuals do not have bands; and 5, selecting a male parent with the genetic sex of XY, and hybridizing the male parent with the common female XX to obtain an all-male offspring XY. According to the invention, the SSR-Y1 marker is adopted, and the SSR-Y1 marker has the advantages of strong specificity, high accuracy, no consumption of a large amount of manpower and low cost; the genetic sex of the catfish is rapidly identified through PCR amplification, the production process of unisexual fish fries of the catfish is simplified by means of a molecular marker-assisted breeding technology, the breeding speed is increased, and the cost is saved.

The invention discloses a method for improving the survival rate of yellow catfish transportation, comprising the steps of: (1) selecting healthy yellow catfish fry to be put into aquaculture water tank, water filling in the water tank, then adding composite polysaccharide nanoparticles and composite probiotics, and the yellow catfish fry is temporarily kept in the tank for 1 3 days without food; (2) the yellow catfish fry temporarily kept without food by water and step (1) are packed in aquatic product special oxygen bag, immediately oxygenating and sealing and ensuring that oxygen content is 6 7mg / L, and the bag is put into the carton of inner foam for transportation; the composite polysaccharide nanoparticles include chitosan, astragalus polysaccharide, oligoxylose, roxburghii polysaccharide and wolfberry polysaccharide and are made. The composite polysaccharide nanoparticles of the present invention are conducive to the absorption of polysaccharide and act on tissue, increase water solubility, dispersibility, and the absorption of fish body thereto, effectively avoid the side effect of polysaccharide accumulation and pollute water body, reduce the time and economic cost added to the feed, thus realize the relief of the fish stress under transportation stress.

This invention discloses a device and method for de-adheding fertilized eggs of the spiny loach. The device includes an incubator with a first guide rail and a second guide rail above it. One end of the second guide rail is connected to the first guide rail via a fixing rod. A first frame is movably disposed between the first guide rails, and a temporary storage box is rotatably mounted on the first frame. A first vertical block is connected to the outer wall of the temporary storage box. A second frame is movably disposed between the second guide rails, and a second vertical block is mounted on the second frame. A first linkage mechanism is provided between the second vertical block and the first vertical block to lock or unlock the connection between the second and first vertical blocks. A second linkage mechanism is also provided between the second frame and the fixing rod to lock or unlock the connection between the second frame and the fixing rod. This device for de-adheding fertilized eggs can regulate the de-adhesion and pouring of fertilized eggs, and evenly distribute the fertilized eggs into the incubator.

The invention provides an ultralow-temperature preservation solution for sperms of mystus nemurus as well as a preparation method and application of the ultralow-temperature preservation solution, and belongs to the technical field of artificial breeding of mystus nemurus. The cryopreservation solution for sperms of mystus nemurus comprises a diluent and acetamide, wherein the final concentration of the acetamide is 80-150g / L; the diluent comprises the following components: sodium chloride, calcium chloride, magnesium sulfate, potassium chloride, glucose, astragalus polysaccharide and taurine. The ultra-low temperature preserving fluid effectively keeps the original pH of semen, maintains the osmotic pressure of the semen and inhibits sperm activation, the addition of acetamide reduces the freezing point of cytoplasm and prevents the formation of ice crystals, and the addition of astragalus polysaccharide and taurine improves the mitochondrial function of sperm and enhances the toughness of cell membranes. The survival rate and the functionality of the frozen sperms are effectively improved.

The utility model relates to the technical field of kitchen utensils, in particular to a fish skin black skin removing device which comprises an inner cylinder, an outer cylinder, a handle, a head fixator and a tail fixator. The inner cylinder is elastically connected with the outer cylinder, and the head fixator and the tail fixator are rotationally connected with the outer cylinder; the head fixator is used for clamping the head of a fish, the tail fixator is used for clamping the tail of the fish, the handle is arranged on the side where the head fixator is located, and the handle is fixedly connected with the head fixator; a skin scraper is arranged on the inner surface of the inner cylinder, and the inner cylinder is formed by rolling up an elastic plate. Fish is placed in the inner cylinder, the head fixing device and the tail fixing device are used for fixing the fish body, the handle is rotated, mucus and black skin on the fish body are scraped off by the skin scraping device, the mucus and black skin treatment efficiency of the device is higher, the treatment effect is better, and the effect is particularly remarkable under the condition of large-scale treatment of the leiocassis longirostris.

The present application relates to long-nosed grass carp breeding technical field, especially green pollution-free long-nossed grass carp breeding method, aiming at the single purification effect of the breeding water in the green breeding process of long-nosed grass carp in the prior art, the present application provides the following scheme, which comprises the following steps: S1, establishing a breeding farm, building suitable breeding ponds according to the breeding scale, and laying bricks and stones or cement hardening treatment on the bottom and side of the breeding pond; S2, establishing a water purification device, installing a certain number of water purification devices in the breeding pond, and regularly starting to purify the breeding water in the breeding pond; S3, prevention and treatment of diseases and insect pests, before putting fish fry, the bottom is comprehensively sprayed with quicklime, the present application not only can conveniently purify the breeding water in the breeding pond, but also can effectively improve the oxygen content in the breeding water in the filtering process, and can also avoid the winding of the water tank on the suction pipe.

This utility model discloses a circulating water purification device for the cultivation of *Cetacea purpurea*, relating to the field of *Cetacea purpurea* cultivation technology. It includes a cultivation box with a flow guide frame at the top and a water storage tank at the bottom. The water storage tank contains a filter assembly, and its inner wall is connected to a vertically arranged water pipe. The beneficial effects of this utility model are: the cultivation box, composed of a frame and a glass plate, allows observation of the *Cetacea purpurea* inside the box from the outside; the flow guide frame, with its spiral structure, utilizes gravity to guide the water flow naturally, reducing power consumption and extending the water flow path, thus improving purification and oxygenation efficiency; the filter assembly purifies the water in the cultivation box; and the ramp and partitions simulate the flow of a natural river through a circulating water system.

1. The name of the designed product: lighter case (sail tail fish). 2. The use of the designed product: used for containing lighters. 3. The design points of the designed product: in shape. 4. The picture or photo that best shows the design points: perspective view 1.

This utility model discloses a high-efficiency and energy-saving ecological breeding pond for *Cetacea purpurea*, relating to the field of breeding pond technology. It includes a frame, with a filter box installed at the top of the frame. The inner wall of the frame is respectively provided with a first inclined plate and a sieve frame. Two sets of water conveying components are provided on the front of the filter box, and a funnel is installed at the bottom of the filter box. A fixing frame is fixed to the inner wall of the frame. The beneficial effects of this utility model are: the filter box can store filter material; when the water conveying components deliver breeding water to the filter box, the water can be filtered through the filter material inside; the first inclined plate can simulate the flow of a natural river through a circulating water system; and the sieve frame can filter the breeding water through the openings in its inner wall, while simultaneously collecting residual feed, feces, and harmful substances in the water.

The utility model discloses a smelly mandarin fish wooden barrel pickling and barreling device which comprises a device main body, a control panel is arranged outside the device main body, a feeding conveying belt is arranged at the top of the device main body, a wooden barrel body is arranged in the device main body, and a material preparation barrel is further arranged in the device main body. The device comprises a material preparation barrel, a material guide barrel is fixedly connected to the bottom of the material preparation barrel, a material pickling bin is fixedly connected to one side of the outer portion of the material preparation barrel, a material spreading box is fixedly connected to the bottom of the material pickling bin, and material scattering holes are formed in the bottom face of the material spreading box. The outer thorns among the mandarin fishes are mutually interspersed, so that the benefit that the integrity of the salted smelly mandarin fishes cannot be ensured is solved. The smelly mandarin fish stacking machine has the technical effects that each smelly mandarin fish can be ensured to keep certain integrity in a manner of stacking the smelly mandarin fish layer by layer by operating the machine.

The present application relates to a kind of miniaturized eel and loach killing machine, belong to eel and loach killing equipment technical field.The miniaturized eel and loach killing machine, including killing box;The inside of the killing box is installed with upper material guide pipe and lower material guide pipe in upper and lower shape;Killing box between upper material guide pipe and lower material guide pipe is installed with cutting knife by cutting motor;The killing box is equipped with conveying mechanism;Conveying mechanism can drive eel and loach to move in the inside of upper material guide pipe and lower material guide pipe when working.The miniaturized eel and loach killing machine is compact in structure, clever in design, using a cutting knife can make the killing machine structure have the function of cutting belly and cutting back, not only save space, but also meet the use demand of multifunctional eel and loach killing, especially suitable for family and restaurant use.

The invention relates to the technical field of microbial fermentation, in particular to a method for preparing leiocassis longirostris oil through salting fermentation. The fish oil is produced by using the leiocassis longirostris viscera as the raw material, and the problem of high-value utilization of by-products such as the viscera in the leiocassis longirostris processing process can be solved. Results of the specific embodiment of the invention show that compared with a cooking method and a salting fermentation method, the fish oil can be rapidly produced without high-temperature heating, and the fish oil is brighter in color and better in quality, mainly has lower acid value, peroxide value, moisture and volatile matter content, higher iodine value and higher unsaturated fatty acid content, and has a wide market application prospect.

The utility model discloses a water flow stirring device for ictalurus punctatus fry culture, a plurality of rotating shafts are rotatably mounted in a culture pond, one end of each rotating shaft extends to the outer side of the culture pond, a belt pulley is fixed on each rotating shaft, one end of one rotating shaft is connected with a driving motor, and a belt is arranged between any two adjacent belt pulleys. The driving motor can be started to be matched with the belt to drive the rotating shafts to rotate, the stirring assembly comprises mounting rings fixed to the peripheries of the rotating shafts, a plurality of stirring plates are fixed to the mounting rings, and when the rotating shafts rotate, the stirring plates can be driven to rotate, so that water flow in the culture pond is stirred, and the flowability of water is improved; the stirring assemblies on the multiple rotating shafts and any two adjacent rotating shafts are arranged at intervals, so that water at different positions in the culture pond can be stirred, and the water flow stirring effect is improved. A plurality of through holes are formed in each rotating shaft, water in the culture pond can flow through the through holes in the water flow stirring process, and the water flow stirring effect is further improved.

The invention relates to the technical field of dipsacus giganteus culture devices, and discloses a dipsacus giganteus culture device which comprises a culture pond, a mounting frame is mounted at the top of the culture pond, a release assembly is arranged on the inner wall of the mounting frame, a suction pump is fixedly assembled on the outer wall of the culture pond, and a flow guide assembly is mounted at the top of the suction pump. According to the device, when water in the inner cavity of the culture pond is sucked under the action of the suction pump, the water can be released into the inner cavity of the collecting box through the water discharging pipe, and in the process that the water flushes the water discharging pipe and enters the collecting box, the oxygen content in the water is increased, and the water is guided and released; and the release position of the connecting pipe can be adjusted according to the height of the water surface, so that severe mechanical impact on the water surface in the oxygenation release process is avoided, the influence of mechanical impact on gill filaments of the dipsacus dipsacus is further avoided, and the risk of stress reaction or disease of gill is reduced.

The invention provides a molecular marker combination for sex identification of glyptosternon maculatum, 53 SNP / INDEL sites closely linked with sex are screened, and the molecular marker combination comprises at least one of the following molecular markers. The unisexual breeding of the glyptosternon maculatum is facilitated, and the breeding process is accelerated.

The invention belongs to the technical field of fermentation processing of aquatic products, and particularly relates to a low-salt freshness-enhancing siniperca chuatsi processing method based on synergistic fermentation of functional complementary lactic acid bacteria. According to the method, endogenous lactic acid bacteria are separated from siniperca chuatsi, the salt tolerance and metabolic characteristics of the endogenous lactic acid bacteria are evaluated in the implementation process, and a lactic acid bacteria combination with complementary functions is preferably constructed. Through the synergistic fermentation effect, degradation of protein and lipid in fish meat is promoted, flavor substances beneficial to formation of delicate flavor are generated, and the delicate flavor and the overall quality of the smelly mandarin fish are improved under the condition that the use amount of salt is remarkably reduced. Compared with high-salt natural fermentation and low-salt natural fermentation, the smelly mandarin fish prepared by the method disclosed by the invention has higher umami response, better texture characteristics and lower pH value and water activity. The method disclosed by the invention is beneficial to improving the stability and safety of the product, and is suitable for standardized and large-scale production of the low-salt-odor mandarin fish.

The application discloses a kind of SSR and SNP molecular marker primer combination for genetic identification of southern catfish family and application thereof, belong to molecular biology field, the SSR molecular marker primer has 9 pairs, nucleotide sequence as shown in SEQ ID No.1-SEQ ID No.18;The SNP molecular marker primer has 6 pairs, nucleotide sequence as shown in SEQ ID No.19-SEQ ID No.30.The application uses 9 microsatellite sites and 6 southern catfish gene SNP mutations as molecular markers, which can distinguish different families of southern catfish, and construct the genetic pedigree of the breeding southern catfish family.The technology can lay a foundation for improving the germplasm purity of southern catfish breeding population and scientific breeding.The method of the application has the characteristics of simple operation and high accuracy.

According to the InDel specific molecular marker for identifying the genetic sex of the megalobrama amblycephala and the application, a primer pair is designed, sample genome DNA serves as a template, the genetic sex of the megalobrama amblycephala can be identified only through PCR and agarose gel electrophoresis, consumed time is short, damage to fish bodies is small, limitation of fish ages is avoided, the InDel specific molecular marker can be applied to the whole life cycle of the megalobrama amblycephala, and the application range of the InDel specific molecular marker is wide. The method is convenient and rapid, has higher accuracy, and is especially suitable for rapid sex determination of a large number of samples. The method provides basic data for further mining of the sex determining gene of the megalobrama amblycephala, and lays a foundation for sex control breeding of the megalobrama amblycephala.

The utility model relates to the technical field of kitchen utensils, in particular to a pair of special cutting scissors for leiocassis longirostris, which comprises two outer cutter arms and two inner cutter arms, the two outer tool arms are respectively arranged on the outer sides of the two inner tool arms; the outer tool arm is provided with a first handle; the inner tool arm is provided with a second handle; the outer tool arms rotate with the fixed shaft as the center, and the two inner tool arms are relatively fixed and rotate with the fixed shaft as the center. A first blade is arranged on the outer side of the inner tool arm, and a second blade is arranged on the inner side of the outer tool arm. A double-scissor structure is formed by the two outer cutter arms and the two inner cutter arms, firstly, a kitchen knife is used for cutting down a fish head, the two inner cutter arms extend into a fish bone below gills in a fish body, the two outer cutter arms are pinched down, the fish bone is clamped between the outer cutter arms and the inner cutter arms, and the fish bone is pinched off through shearing force. The scissors slide towards the tail part of the fish along the pinching-off part of the fish bone, so that the whole fish belly can be cut, and a swim bladder cannot be scratched.

This utility model discloses a channel catfish hatching device, relating to the technical field of hatching equipment. The utility model includes a hatching chamber with an operating table. Multiple cavities are arranged on the operating table surface, and hatching components are selectively placed in these cavities. Each hatching component consists of a flowing water hatching tank and multiple hatching troughs. An overflow port is provided on the side wall of the flowing water hatching tank, and a fry discharge pipe is provided on the end wall of the flowing water hatching tank. This utility model sets up independent flowing water hatching tanks on the operating table, draining water through the drain pipe and releasing fish through the fry discharge troughs. Each flowing water hatching tank forms an independent hatching environment. The farmer only needs to periodically inspect the hatching chamber, shake the hatching troughs when necessary, and remove the plugs when releasing fish, simplifying the hatching process and operation. It also solves the problem that existing devices cannot be used to hatch different batches of channel catfish fertilized eggs.

This invention relates to the field of black-spotted catfish farming technology, and in particular to a method and apparatus for black-spotted catfish farming. The apparatus includes a farming pond, a sludge treatment component, a temperature control module, a drive component, and a heat-insulating shed component. The sludge treatment component is fixedly connected to the farming pond, and the temperature control module is fixedly connected to the inner wall of the farming pond. The catfish are placed in the farming pond for farming, and the temperature is controlled at 8℃-9℃ by the temperature control module. When a large amount of fish excrement and debris is generated at the bottom of the pond, the drive component activates the sludge treatment component to collect and clean the sludge and clean the hourglass farming plate, preventing blockage of the hourglass farming plate and avoiding water quality issues and fish diseases. When the weather is cold or too hot, or when the temperature exceeds the minimum and maximum thresholds, the temperature control module activates the heat-insulating shed component to maintain a stable water temperature in the farming pond and ensure an optimal farming environment.

The application provides a method for large-mouth catfish fry conversion feeding, and belongs to the technical field of large-mouth catfish fry conversion feeding. The method for large-mouth catfish fry conversion feeding provided by the application feeds water worms at the beginning of the fry conversion feeding stage, then feeds pig liver for fry cultivation, and then feeds conversion feed made of pig liver and large-mouth catfish fry powder in different proportions. Through 25-36 days of step-by-step conversion feeding, the fry successfully feeds on artificial compound feed, and the fry size grows from the initial 0.5g / fish-1.7g / fish to 7g / fish-12g / fish. The large-mouth catfish fry conversion feeding is completed, and the fry conversion feeding survival rate can reach more than 89.5%. Meanwhile, the method provided by the application divides the fry into different sizes for feeding by screening the fish once every 6-9 days during the conversion feeding cultivation process, so that the self-cannibalism caused by large size difference is effectively prevented.

The application discloses a method for thawing frozen channel catfish fillets by using magnetoelectric coupling, and comprises the following steps: placing the frozen channel catfish fillets into a sealed bag and placing the sealed bag into a magnetoelectric coupling freezing and refrigerating box; inserting a thermocouple temperature probe into the center of the meat sample to monitor the temperature in real time; and stopping the magnetoelectric coupling when the temperature reaches a predetermined temperature, thereby completing the thawing. The method can reduce the damage of the channel catfish during thawing, improve the quality stability of the thawed channel catfish, and has high thawing efficiency.

The invention discloses an application of a self-assembled bio-based composite preservative film and a film-covering preservation method of leiocassis longirostris, and relates to the field of aquatic product processing and preservation. According to the method, fresh and alive leiocassis longirostris is used as a raw material, is pre-treated, is wrapped by a composite coating, and is placed in a refrigerator at 4 DEG C for refrigeration. According to the method disclosed by the invention, a green composite coating is applied, and the coating has excellent antibacterial and antioxidant effects. In addition, compared with a non-degradable plastic preservative film, the bio-based composite coating is easier to accept by consumers due to the characteristics of safety and no pollution.

The invention relates to a channel catfish pectoral fin positioning and cutting device and method. The channel catfish pectoral fin positioning and cutting device is provided with a fish carrying assembly, a fin positioning assembly and a cutter assembly. Through fish mouth positioning, a nitrogen spring in the head direction and thrust acting on a brush roller on pectoral fins, positioning of a fish body in the pectoral fin arranging and cutting process is achieved, the thrust of the brush roller on the fish loses efficacy after fin cutting, and a fish carrying assembly is reset; in the movement process of the electric cylinder, the machining state is judged based on data changes of the pressure sensor, the distance and the rotating speed of the brush rollers on the two sides are adjusted in real time, and movement control over the brush rollers is achieved. In the aspects of fish body positioning and motion control, pectoral fin arrangement, fish feeding and fin cutting of objects of different specifications and sizes can be achieved only through cooperation of an electric cylinder and a synchronous belt driving mechanism. Wherein in the pectoral fin finishing stage, the distance between two brush rollers is minimum, so that fin separation is facilitated; in the fin cutting stage, the distance between the two brush rollers is the largest, so that the positions of the two brush rollers and the rotary cutter are staggered, and the rotary cutter is prevented from interfering with the brush rollers.

1. Name of the designed product: hair dryer (catfish). 2. Use of the designed product: for drying and styling hair. 3. Design points of the designed product: in shape. 4. Picture or photo that best shows the design points: perspective view.