110 results about "Ictalurus catus" patented technology

The present invention discloses one kind of fast frozen white delicious fish fillet of spotted forked-tail Leiocassis longirostris and its production process. The production process includes the steps of temporarily raising, killing to eliminate blood, slicing, eliminating skin, trimming, soaking to sterilize, loading into tray and quick freezing, grading, coating with ice, packing, etc. The production process features complete elimination of blood, low temperature of fish fillet in icy water at 0-4 deg.c, soaking fish fillet in ozone icy water and ultralow temperature quick freezing at below -35 deg.c. The production process has high fish fillet yield and less nutrient loss, and the produced fish fillet is white, translucent and delicious.

The invention discloses feed for controlling medicine residues of channel catfishes, which comprises the following components in the mass percent: 13.85% of puffed soybean meal, 4.0% of fermented soybean meal, 12.0% of steamed fish meal, 18.0% of rapeseed meal, 16.5% of cottonseed protein, 16.0% of rice bran, 14.32% of secondary powder, 1.8% of calcium biphosphate, 2.0% of IV-grade soybean oil, 1.0% of premix compound, 0.01% of vitamin E, 0.02% of vitamin C, 0.05% of high-temperature resistant complex enzyme preparation, 0.05% of cure accelerator and 0.4% of Chinese herbal medicine additive, and the total percent is 100%. By optimizing the components of the feed, adding Chinese herbal medicines in the feed to replace antibiotics and adding high-temperature resistant enzyme preparations, biological live bacterial preparations and the like, the purposes of improving the stability of the feed, promoting the health growth of channel catfishes, enhancing the immunity of the physique and theorganisms of fish bodies and preventing diseases are achieved, thereby finally achieving the purposes of improving the growth rate of channel catfishes, reducing the feeding cost, discarding antibiotics and controlling the medicine residues in bodies under the normal water quality environment.

The invention discloses a channel catfish immunity enhancing feed, which comprises the following components in parts by weight: 5-14 parts of meat and bone meal, 15-25 parts of rapeseed meal, 20-40 parts of de-hulled soybean meal, 0.2-0.5 part of choline chloride, 15-22 parts of wheat middling, 0.5-1 part of soybean oil, 0.2 part of vitamin premix, 0.15 part of mineral premix, 1-2 parts of calcium dihydrogen phosphate, 0.01-0.03 part of plant enzyme, 0.05-0.1 part of cholate substances, 0.03-0.06 part of a toxin adsorbent, 0.1-0.3 part of a natural plant compound and 0.1-0.3 part of a polysaccharide compound. According to the immunity enhancing feed disclosed by the invention, nonspecific immunity of the channel catfish is enhanced through various compounds, and through reasonable proportioning of various natural plants and the polysaccharide components, nonspecific immunity of the channel catfish is significantly improved and infection resistance of the fish is enhanced; and meanwhile, growth performance of the fish is improved and a food coefficient is reduced.

The invention relates to environment protection fish-meal-free spot ietalurus punetaus compound feed, which belongs to the field of compound feed and processing thereof. The compound feed of the invention comprises the following ingredients in mass percent: 38 to 53 percent of bean dregs, 5 to 20 percent of corn protein powder, 0 to 15 percent of cotton dregs, 0 to 15 percent of vegetable dregs, 15 to 24 percent of wheat shorts, 1 to 2.5 percent of cellulose, 0.1 to 0.3 percent of compound amino acid, 0.05 to 0.1 percent of immunity enhancing agents, 0.3 to 0.5 percent of lycine, 2 percent of fish oil, 0.3 percent of choline chloride, 0.1 percent of vitamin C, 0.15 percent of multidimensional premix compounds, 0.1 percent of polymineral premix compounds and 0.03 percent of phytase (5000 u/g). The preparation method comprises the preparation processes of raw material crushing, mixing, extruding pelletization, vacuum spray coating and baking. The invention has the advantages of simple preparation, low price and balanced nutrition, can meet the growth requirement of the ietalurus punetaus, can reduce the production cost, can improve the assimilation and the utilization of nutrient substances, and can reduce the water body pollution caused by nitrogen and phosphor discharge in the culture process.

The invention discloses a Ictalurus punctatus compound feed, the contents of mass ratio of characteristic materials are as follows: vitamin A 0.005-0.007, vitamin D3 0.004-0.006, vitamin E 0.070-0.090, vitamin K3 0.002-0.004, vitamin B1 0.003-0.005, vitamin B2 0.005-0.007, vitamin B6 0.08-0.012, vitamin B12 0.001-0.003, nicotinamide 0.030-0.050, calcium pantothenate 0.10-0.30, folic acid 0.01-0.03, biotin 0.001-0.002, VC-phosphate 0.300-0.400, inositol 0.070-0.090, antioxidants 0.200-0.300, wheat-middlings 1.000-1.200, ferrous sulfate 0.400-0.600, copper sulfate 0.010-0.030, zinc sulfate 0.150-0.200, manganese sulfate 0.100-0.150, iodine 0.050-0.150, Se 0.050-0.070, cobalt chloride 0.010-0.030, magnesium sulfate 1.000-1.500, zeolite powder 5.0-6.0, xylo-oligosaccharide 0.008-0.012, bacillus licheniformis 0.01, weatings 90.-110., wheat-middlings 110-130, rice bran 75-85, soy bean powder 55-65, soybean meal 190-210, peanut meal 25-35, fish meal 95-105, cottonseed meal 75-85, rapeseed meal 110-130, gem meal 45-55, soybean oil 15-25, lysine 0.8-1.2, methionine 0.8-1.2, salt 2.0-2.5, betaine 3-5, allicin 0.1-0.3, choline chloride 1.0-2.0, calcium dihydrogen phosphate 15-25. Compared with other feeds, the usage rate of feed nutrient ingredients for Ictalurus punctatus is increased by 10-15%, feed coefficient is reduced by 5-10%, feed waste is reduced, nitrogen and phosphorus excretion of Ictalurus punctatus in the environment is reduced, at the same time the survival rate of Ictalurus punctatus is improved significantly.

The invention belongs to the technical field of channel catfish skin processing, and particularly discloses a method for processing channel catfish skin. The method comprises the process flow as follows: cleaning, sectioning, deodorizing and rinsing, soaking for flavor, pre-baking, microwave puffing, seasoning, packaging and sterilizing the channel catfish skin, so as to achieve the finished product. The fish skin is golden yellow, highly lustrous and crisp. Black tea has an excellent deodorizing effect. The seasoning liquid seasons the fish skin with tasty flavor, the product is spicy and odorless, has the color of orange red chili oil except the silver gray color of the fish skin, and is enticing in color and flavor. The processed fish skin has the total bacteria no greater than 10 cfu/g, and the coliform no greater than 30 MPN/100G, is free of pathogenic bacteria and the food spoilage caused by biological action, and can be guaranteed in quality more than 6 months under normal temperature.

The invention discloses a method for preparing a high-calcium soup-stock fish ball by using channel catfish bone, which is characterized by comprising the following steps of: removing, head, tail and side wings of a fish skeleton, remaining a fish steak and ensuring that the fish skeleton has components with black skin; rinsing the fish skeleton with clean water, draining and slightly freezing the fish skeleton at 0 DEG C; crushing the slightly-frozen fish skeleton by a crusher and grinding the powder by colloid into fishbone crushed meat with the granule no more than 1mm; centrifuging and dewatering the ground fishbone crushed meat for later use; preparing the fishbone crushed meat for later use, accessories and seasonings together into an outer coating material; and evenly stirring the outer coating material with pork emulsion, sugar, salt and agar to be prepared into stuff core, placing the stuff core and the outer coating material into a forming machine for shaping and then boiling the shaped material with water to cure to obtain fish balls. By the method, the added value of products can be effectively improved, and the high-efficiency comprehensive utilization of the products can be promoted.

The invention discloses an extraction process for collagen of channel catfish skin, which comprises the following steps: firstly, impure protein of channel catfish skin scrape is removed, then channel catfish skin is decolorized, and finally breaking treatment, enzyme hydrolysis, enzyme inactivation, concentration treatment, low-temperature drying and grinding treatment are carried out on channel catfish skin to obtain the finished products. The extraction process finely and further processes and comprehensively utilizes the channel catfish skin scrape; the purity of extracted collagen II type product can reach more than 90%; the effect of scrape additional value is specially obvious; the product has wide usage scope; the whole extraction process does not have heavy metal proof, residual medicine, is healthy and safety; after being processed, the product does not have plane-source environment and water source pollution; and the extraction process flow and the device needed by the process flow are simple and have low manufacturing cost.

The invention discloses a recycling technology of leftovers of ictalurus punctatus. In the technology, the leftovers of the ictalurus punctatus are mainly adopted to extract finished products of fish bone powder, albumen powder and unsaturated fatty acid through separating and drying by the method of enzyme hydrolysis by regulating pH value and temperature. In the technology, deep processing and comprehensive utilization are carried out on the fish bone powder; the products obtained by the recycling technology are free of heavy metals over standard and drug residues and are sanitary and safe; after processing, the wastewater produces no non-point pollution and water pollution; the equipments and flows required by the technology are simple and the production cost is low.

The invention discloses a microsatellite family identification method for ictalurus punctatus. The microsatellite family identification method comprises the following steps: (1) breeding an ictalurus punctatus family by adopting an artificial propagation method, and carrying out polyculture on 100 family descendants in one pond; (2) analyzing gene types of an ictalurus punctatus breeding group on 18 microsatellite marker sites by adopting a fluorescence marking primer and a multi-PCR (Polymerase Chain Reaction) amplification method; screening 10 effective microsatellite markers which have high polymorphism and are suitable for family identification; (3) optimizing a multi-PCR amplification system of the 10 microsatellite markers and analyzing gene types of the polycultured descendants on 10 microsatellite marker sites; (4) identifying a parental origin of each descendant according to the gene types of parents and the descendants. According to the microsatellite family identification method disclosed by the invention, a paternity testing method is established for the ictalurus punctatus for the first time and the identification accuracy reaches 96.6 percent; polyculture individual parents of the ictalurus punctatus can be rapidly and accurately identified. The microsatellite family identification method disclosed by the invention can be used for rapidly and accurately carrying out family identification on the polyculture individual parents of the ictalurus punctatus, and the breeding effect is improved.

The invention relates to a hot and spicy sauce containing Ictalurus Punctatus fishbone, and a preparation method thereof, and belongs to the technical field of food production. The raw materials of the hot and spicy sauce comprise a fishbone spicy paste, Ciba chili, pickled pepper, soy, a broad bean sauce, fermented soya bean, white sesame, white granulated sugar, monosodium glutamate, ten-medicine Xiang and potassium sorbate. The preparation method concretely comprises the following steps: heating fragrance oil, adding the pickled pepper, stir-frying, dumping the soy, heating to boiling, adding the broad bean sauce, the Ciba chili, the fishbone spicy paste, the fermented soya bean, the white sesame, the white granulated sugar, the monosodium glutamate, the fishbone spicy paste and potassium sorbate, carrying out mixing frying while continuously stirring, adding a proper amount of purified water to form a sauce, canning to a bottle the sauce while hot, sterilizing, and cooling to obtain the hot and spicy sauce. The hot and spicy sauce containing Ictalurus Punctatus fishbone enriches the kind of Ictalurus Punctatus fishbone foods, solves the present situation of the only utilization of the Ictalurus Punctatus fishbone as a feed raw material, is in favor of the culturing popularization of the Ictalurus Punctatus fishbone, improves the added values of the hot and spicy sauce, and promotes the efficient comprehensive utilization of the Ictalurus Punctatus.

The invention discloses a feed capable of preventing the fading of spots of channel catfish. The feed capable of preventing the fading of the spots of the channel catfish comprises the following components in percentage by mass: 15.5% of expanded soybean meal, 4.2% of fermented soybean meal, 15.0% of Peruvian fishmeal, 16.0% of rapeseed meal, 15.65% of cottonseed protein, 14.6% of rice bran, 13.0% of wheat middling, 1.8% of monocalcium phosphate, 1.0% of soya-bean oil, 1.5% of phospholipid oil, 1.0% of a premix, 0.02% of vitamin E, 0.03% of vitamin C, 0.05% of complex enzyme, 0.15% of composite bile acid with mass concentration of 20% and 0.5% of traditional Chinese herbal medicine additives, wherein the sum of the mass percentages is 100%. According to the feed capable of preventing the fading of the spots of the channel catfish, the components of the feed are optimized; the composite bile acid and the traditional Chinese herbal medicine components are added according to a certain ratio so as to the effects of improving the capability of the channel catfish body in decomposing and using fat, improving the activity of fatty acid, promoting the emulsification of the fatty acid and the steatolysis effect of pancreas, reducing the content of the cholesterol in the blood, reducing the pressure of the fish body liver for decomposing the fat, activating the activity of tyrosinase, promoting the deposition of melanin and spots and promoting the deposition of the pigment spots and the rapid growth of the channel catfish under the conditions of normal water quality and high-density feeding.

The invention belongs to the technical field of biology, and particularly relates to a preparation method for aeromonas hydrophila outer membrane protein gene prokaryotic expression protein. The method comprises the following steps that the protein clones an outer membrane protein gene segment into a prokaryotic expression vector pET-30a to obtain a recombinant expression vector, the recombinant expression vector is converted into Escherichia coli to obtain recombinant Escherichia coli, the recombinant Escherichia coli is inoculated to an Amp-resistant LB plate to be cultured for OD600 for 0.6 h, IPTG is added to reach the final concentration being 1.0 mmol/l for inducible expression, and the aeromonas hydrophila outer membrane protein gene prokaryotic expression protein is obtained after purification is performed. According to the method, the aeromonas hydrophila outer membrane protein gene prokaryotic expression protein is successfully constructed, the aeromonas hydrophila outer membrane protein gene prokaryotic expression protein can be used as a vaccine capable of protecting fish, the immune effects of the aeromonas hydrophila outer membrane protein gene prokaryotic expression protein pompA for channel catfish under different FIA effects are compared and researched, and a foundation is laid for further researching the pompA and researching whether the pompA can be used as the candidate component of the genetic engineering subunit vaccine or not.

The invention discloses a method for preparing rinsed fish fillets from fillets of ictalurus punctatus Rafinesque. The method is characterized by sequentially comprising preprocessing fish flesh, soaking fish fillets, preparing frozen fish flesh, slicing frozen fish flesh, putting the frozen fish flesh in a slicing machine and then slicing into rinsed fish fillets according to the thickness of the rinsed fillets. By the method, on the one hand, a novel method is found for the deep processing of the fillets of ictalurus punctatus Rafinesque, and the added value of the product is improved; on the other hand, a novel delicacy is provided for the rinsed fillet market. When the fish flesh prepared by the method is sliced and rinsed, the fish flesh is not loosened and broken, and the elastic property is good.

The present invention discloses a manufacturing method of a soft canned seasoned and instant ictalurus punctatus which includes the following steps: pre-treating freshwater fish, removing fishy smell and pickling, drying, oil-frying, seasoning and packaging, and sterilizing and cooling. The soft canned seasoned and instant ictalurus punctatus uses freshwater fish with relatively high fat content as raw materials, the same technology can be popularized and applied in the manufacturing processes of the canned freshwater fish which uses raw materials with the same or similar characteristics, especially with relatively high fat content, and thus the market is enriched.

The invention belongs to a fish artificial incubation method field relating to the artificial incubation method of ictalurus punctatus. After the parent fishes are in natural spawning fertilization in a spawning pond, the egg masses collected are disposed in an incubating plant for hatching, which is characterized in that the hatching water salinity is 0.1 to 8%, the alkalinity and hardness are larger than 20mg/L calcium carbonate, the hydrogen sulfide is smaller than 0.005mg/L, the iron is smaller than 0.2mg/L, the ammonia is smaller than 0.5 mg/L and the dissolved oxygen is larger than 5mg/L. The water temperature should be maintained from 24 degree to 30 degree. The membrane rupture prophase of this incubation method is implemented inside a cement pool or a circuit in which the conditions such as water temperature are relatively stable. After the melanose of eyes are emerged or the egg masses are turned into red, the fries are moved to a hatching tray for the rupture of membrane. Therefore, the fry activities are easy to observe, and the fry collecting can also be facilitated.

Seasoned freshwater fish is prepared from components in parts by weight as follows: 70 parts of channel catfish, 80 parts of water, 1.5 parts of welsh onions, 1.5 parts of ginger, 1.25 parts of Erguotou, 2 parts of onions, 0.5 parts of coriander, 1 part of celery, 0.2 parts of dried chilies, 0.5 parts of garlic, 0.1 parts of dried Sichuan pepper, 0.2 parts of pepper, 0.1 parts of cinnamon, 0.2 parts of cloves, 0.1 parts of dahurian angelica roots, 0.2 parts of liquorice, 0.1 parts of perilla frutescens, 0.2 parts of peach blossoms, 0.1 parts of lilies, 0.2 parts of pepper leaves, 0.1 parts of platycodon roots, 0.1 parts of date pits, 0.2 parts of okra seeds, 0.2 parts of dried red bayberries, 0.1 parts of Chinese yams, 0.2 parts of dang shen, 0.1 parts of Angelica sinensis, 0.2 parts of Gynostemma pentaphyllum and 0.1 parts of senna leaves. The raw materials are low in cost, the process is simple, industrial production is facilitated, the added value of fish by-products is increased greatly, and the market prospect is broad.

The invention relates to a fodder for channel catfish, and particularly relates to a health care fodder capable of preventing channel catfish from dying during overwintering and a preparation method thereof. The health care fodder comprises the following raw materials by percentage by weight: 15%-40% of concentrated feed, 1%-8% of fish meal, 5%-20% of dephenolized cottonseed protein, 5%-20% of wheat, 5%-25% of wheat middling, 2%-10% of meat and bone meal, 1%-3% of zeolite powder, 1%-3% of fish oil, 0.4%-2.5% of nutrition additive, 0.05%-0.1% of toxin adsorbent, and 0.02%-0.03% of antioxidant. The health care fodder can improve the appetite of the channel catfish, allows the channel catfish to grow rapidly, enhances the immunity of the channel catfish, effectively improves the fatness and weight of the channel catfish, and improves the intestinal health of the channel catfish; and after overwintering, the survival rate of the channel catfish reaches over 80%, which is improved by more than 55% compared with that of the channel catfish fed with common fodder.

The invention relates to an identification method of channel catfish species. The identification method comprises following steps: genome DNA of channel catfish muscular tissues is extracted; a PCR primer is designed in mitochondrial DNA D-loop region; the genome DNA is subjected to PCR amplification with the presence of the PCR primer so as to obtain mitochondrial DNA D-loop sequences; the mitochondrial DNA D-loop sequences are subjected to enzyme digestion for 2 to 3h at a temperature of 37 DEG C by using restriction enzyme EcoR I; and then the mitochondrial DNA D-loop sequences are subjected to agarose gel electrophoresis after enzyme digestion, and the channel catfish species are identified according to the results of electrophoresis. Enzyme sites are searched based on sequencing of D-loop, and enzyme digestion identification is performed, so that sequencing is not necessary for identification of channel catfish species. The identification method is capable of identifying channel catfish species at a molecular level; operation is simple and easy; consumed time is short; and identification method is suitable for species identification of channel catfish in import and export trade.

The invention discloses a preparation method of channel catfish roe sticks. The preparation method is characterized by comprising the following steps: placing frozen channel catfish roes in salty water for defreezing, soaking with salty water, removing capsule dressing, rinsing in clear water, taking out and leaching as standby materials; adding seasoning liquid in the standby materials, standingfor certain time, adding seasoning accessories, and evenly mixing to obtain standby seasoning materials; steaming the standby seasoning materials with steam to obtain cooked materials; adding bindingagent into the cooked materials, evenly mixing, extruding into shuttle stick type finished products through a forming machine; and packaging the shuttle stick type finished products in a mode of vacuum, and carrying out high-temperature disinfection to obtain the channel catfish roe sticks. The preparation process is simple and reasonable, the product is delicious in taste, fragrant in flavor, unique for flavor, proper in mouthfeeling, abundant in nutrition, convenient and ready to eat, enriches the varieties of fish roe products, and the product additional values can be increased, and the comprehensive utilization of the channel catfish can be improved.

The invention discloses a method for carrying out integrated aquaculture of ictalurus punctatus in a pond industrial aquaculture system. The method is scientific and feasible in principle, easy to operate and implement in production, and capable of being adopted and applied by farmers easily in economic benefit. The method comprises the steps of preparation before planting and aquaculture, fry stocking, purification zone floating bed rice planting, planting and aquaculture management, aquatic species capturing and rice harvesting. Accordingly, by means of the proper stocking density of ictalurus punctatus fingerlings in an aquaculture water tank, the stocking size is improved, and by means of the annual aquaculture, commercial longsnout catfish with the best-selling size being about 1.5 kg/fish are sold at a high price at different times; by properly stocking a proportion of silver carp, bighead carp, myxocyprinus asiaticus, penaeus monodon and snails and planting 5-8% of floating bedrice in an ecological purification zone simultaneously, and by means of the integrated planting and aquaculture measure, the in-situ remediation effect on aquaculture water is better exerted, the ecological benefit, comprehensive output and economical benefit of the whole breeding system are significantly increased, and the method has strong practical application and popularization value.

The invention relates to a method for intensive cultivation of ictalurus punctatus. The method comprises a step (1) of performing fingerling cultivation, a step (2) of performing adult fish cultivation, a step (a) of having pond conditions; a step (b) of performing preparation prior to stocking; a step (c) of performing stocking of fingerlings; a step (d) of feeding baits: adopting mixed domestication feeding of compound pellet feed and Chinese herbal medicine baits; a step (e) of performing daily management, and timely increasing the feeding amount of the Chinese herbal medicine baits by 15-20% of an original usage amount when fish disease is found. The Chinese herbal medicine baits are prepared from, by weight parts, 3-5 parts of nucleic acid yeast extract, 0.5-1 part of multi-vitamins, 10-15 parts of Chinese herbal medicine extract, 40-60 parts of enteric soluble polymers and 5-8 parts of vitamin E acetic ester. The Chinese herbal medicine baits used in the cultivation method are reasonable in compatibility, balanced in nutrition and low in processing cost, can remarkably improve immunity of ictalurus punctatus and greatly reduce the probability of edwardsienosis infection of ictalurus punctatus, is free of drug residue, leads to no drug resistance and is high in safety.

The invention belongs to the biological technical field, and particularly relates to aeromonas hydrophila outer membrane protein gene prokaryotic expression protein and an application thereof. An outer membrane protein gene segment is cloned into a prokaryotic expression vector pET-30a by the protein, a recombinant expression vector is obtained, the recombinant expression vector is converted into escherichia coli to obtain recombinant escherichia coli, the recombinant escherichia coli is inoculated into an Amp resistant LB panel to be subjected to cultivating until OD 600 reaches 0.6, IPTG is added, the final concentration reaches 1.0 mmol/L, inducible expression is carried out, and after purification is conducted, the aeromonas hydrophila outer membrane protein gene prokaryotic expression protein is obtained. The aeromonas hydrophila outer membrane protein gene prokaryotic expression protein is obtained successfully, the aeromonas hydrophila outer membrane protein gene prokaryotic expression protein can serve as vaccine which has the protecting effect on fishes, the immune effects on channel catfish by the aeromonas hydrophila outer membrane protein gene prokaryotic expression protein pompA under the different FIA effects are compared and researched, and the basis is provided for the further research whether ompA can serve as a candidate ingredient of genetic engineering subunit vaccine or not.