According to the high-temperature-resistant
nitrogen-fixing
bacteria microcapsule
fertilizer and the preparation method thereof, aiming at the problems of
thallus inactivation and
nutrient loss in a high-temperature environment, a
sodium alginate-
chitosan double-layer embedding technology is combined with magnetic
nanoparticle modification, and a stable double-layer structure is formed by optimizing the ratio of an embedding material, so that the high-temperature-resistant
nitrogen-fixing
bacteria microcapsule
fertilizer is prepared. The
survival rate and activity of the
nitrogen-fixing
bacteria at high temperature are remarkably improved, and meanwhile, the functions of intelligent
nutrient release and magnetic
recovery are achieved. The
fertilizer is prepared from composite microcapsules,
amino acid,
humic acid and other raw materials according to a specific ratio through
magnetic field orientation treatment, multi-stage mixing, atomization
granulation and
magnetic field screening processes, the particle size is controlled to be 0.5-3.35 mm, and the
shelf life is prolonged to 12 months. The method has the technical effects that
trehalose-
glycerol /
sorbitol synergistically forms a glassy protective film to maintain the stability of thalli, gamma-
aminobutyric acid activates
nitrogenase gene expression,
magnetic nanoparticles construct a photosynthetic symbiotic
system to optimize
rhizosphere oxygen supply, and silanized FeO nanoparticles fix heavy
metal ions through
electrostatic adsorption, so that the heavy
metal ions in the thalli are effectively adsorbed, and the heavy
metal ions in the thalli are effectively adsorbed. And the
humic acid buffers the pH fluctuation of the soil, so that the
nitrogen fixation efficiency is finally improved by 30-50%, and the
crop yield is increased by 15-25%.