58 results about "Leiocassis longirostris" patented technology

The present invention discloses one kind of fast frozen white delicious fish fillet of spotted forked-tail Leiocassis longirostris and its production process. The production process includes the steps of temporarily raising, killing to eliminate blood, slicing, eliminating skin, trimming, soaking to sterilize, loading into tray and quick freezing, grading, coating with ice, packing, etc. The production process features complete elimination of blood, low temperature of fish fillet in icy water at 0-4 deg.c, soaking fish fillet in ozone icy water and ultralow temperature quick freezing at below -35 deg.c. The production process has high fish fillet yield and less nutrient loss, and the produced fish fillet is white, translucent and delicious.

The invention discloses a Leiocassis longirostris breeding system for adjusting the feeding amount on the basis of weight detection. The feeding system includes: an acquisition unit for acquiring fish pool internal whole infrared image information; a storage unit for storing a corresponding list of fish volume summation and feeding amount summation; a feeding unit for using a plurality of feeding device to throw a target feeding amount of food to a fish pool; a shooting unit for acquiring the fish pool internal whole infrared image information; a control unit for acquiring the fish pool internal whole infrared image information, calculating the volume summation of fishes in the fish pool, searching the corresponding feeding amount summation, and sending the feeding amount summation to the feeding unit, wherein the control unit acquires the fish pool internal whole infrared image information, analyzes fish distribution in the fish pool, and controls the plurality of feeding device to act through a command according to the fish distribution. The breeding system adopts regional feeding, can provide a healthy feeding environment for fish, can improve the growth speed and reproduction speed of fish, and can ensure the breeding effect and efficiency of fish.

The invention provides a primer group for identifying a male parent and a female parent of tachysurus fulvidraco and leiocassis longirostris hybrid species. The primer group comprises a primer pair for amplifying COI genes and a primer pair for amplifying an ITS sequence, wherein the primer pair for amplifying COI genes consists of a primer COIF and a primer COIR; the primer pair for amplifying an ITS sequence consists of a primer ITSF and a primer ITSR. The invention also provides a kit which is for identifying the male parent and the female parent of tachysurus fulvidraco and leiocassis longirostris hybrid species and comprises the primer group. The kit also comprises any one or more of the following reagents: a buffer solution for PCR, dNTP, heat-resistant DNA polymerase, a cloning vector, a DNA ligase, and a buffer solution for ligation. The invention also provides a method for identifying the male parent and the female parent of tachysurus fulvidraco and leiocassis longirostris hybrid species. The primer group, the kit or the method can be used for accurately identifying the male parent and the female parent of the tachysurus fulvidraco and leiocassis longirostris hybrid species, thus effectively managing a breeding plan and monitoring negative influence thereof, and has great significance in sustainable development of aquaculture industry.

The present invention discloses a complete nutrient pelletized feed for longnosed wei fish. Said feed is formed from fish powder, soybean cake, rape cake, corn flour, edible oil, mixed vitamins, mixed mineral salt and additive.

The invention relates to a purse seine cultivation method, in particular to a purse seine high-efficiency ecological cultivation method, which adopts a three-layer three-dimensional mixed cultivation method, wherein the upper layer water body cultivates silver carps; the middle layer water body cultivates Taihu whitefish or squaliobarbus curriculus first, and then cultivates catfish; and the lower layer water body cultivates hemibarbus maculatus bleeker, and leiocassis longirostris or pseudobagrus fulvidraco first, and then cultivates turtle. The method selects proper fish species and breeding density according to the eutrophication degree of the water body and the food habits and the life habits of fishes, does not put feedstuffs, baits or fertilizers into the water body in the whole cultivation process, makes full use of the water body natural resources, changes the waste into valuable and harmful into beneficial, and reduces the water body pollution, thus the cultivation cost of aquatic products is low, the quality of the aquatic products is high, and the economic benefit is good. The method solves the technical problems that most of the prior purse seine cultivation species mainly comprise the fishes which are cultivated with feedstuffs, cause water quality eutrophication to pollute the water body, and cause ecological balance disturbance.

The invention relates to a special slow-falling extruded mixed feed for Leiocassis longirostris, and a preparation method thereof. The special slow-falling extruded mixed feed for Leiocassis longirostris comprises, by mass, 30-40% of fishmeal, 15-25% of soybean meal, 5-10% of degossypolized cottonseed meal, 5-15% of corn protein powder, 5-10% of bread flour, 3-5% of cassava modified starch, 3-5% of sea-tangle powder, 1-2% of calcium dihydrogen phosphate, 3-8% of fish oil, 0.1-0.3% of a Leiocassis longirostris multi-vitamin premix, 0.5-1% of a multi-ore premix, 0.1-0.5% of choline, 0.2-0.5% of salt, 0.01-0.05% of an antioxidant and 0.05-0.2% of a mildew inhibitor. The feed has the following beneficial effects: 1, the nutritional requirements of the Leiocassis longirostris are well satisfied, and the growth speed is fast; 2, the feed is suitable for the intake of the Leiocassis longirostris; and 3, the digestion utilization rate is high, the nitrogen and phosphorus discharge is low, and the pollution to water is small.

The invention relates to a compound Chinese herbal medicine preparation, in particular to a compound Chinese herbal medicine preparation for preventing and treating the oosperm block saprolegniasis of spot furcate-tail leiocassis longirostris, which is applied to the incubation process of fish class artificial propagation oosperm. The compound Chinese herbal medicine preparation respectively comprises the following components by weight percent: 14.5% to 20% of kuhseng, 13.5% to 18% of cnidium fruit, 8% to 13% of aloe, 13.5% to 18% of kaempferia galamga, 13.5% to 18% of broom cypress fruit, 8% to 13% of bunge prickly ash, 8% to 13% of nutgall and 14.5% to 20% of smilax glabra roxb. The invention has the following effects: 1, the raw materials are matched according to the matching principle of Chinese herbal medicines, the preparation reinforces the medicine effect, does not damage the embryonic development of the fish class oosperm and has especially obvious effect for preventing and treating the saprolegniasis in the incubation process of the oosperm; 2, the medicines are degradable under the condition of a water body, have no toxic or side effect and do not influence the normal embryonic development of roe, and the muscle of incubated seed bodies does not generate heavy metal and medicine residues; 3, the invention has no corrosiveness and simple and safe using method; and 4, surface source environmental contamination to the water body is not generated.

The invention discloses feed with a function of improving stress resistance of leiocassis longirostris in a later aquaculture period. The feed is prepared from, by weight, 20-30 parts of cicada pupa powder, 10-30 parts of soybean meal, 5-15 parts of wheat middling powder, 5-15 parts of squid powder, 5-10 parts of red fishmeal, 5-12 parts of algae powder, 10-20 parts of flour, 2-10 parts of modified casein, 2-7 parts of fish oil, 1-3 parts of choline chloride, 1-2 parts of bile acid, 1-5 parts of monocalcium phosphate, 0.1-0.5 part of ferric methionine, 0.1-0.5 part of copper sulfate, 0.1-0.5 part of manganese sulfate, 0.1-0.5 part of zinc methionine, 0.1-0.5 part of magnesium sulfate, 0.5-1 part of arginine, 0.1-0.5 part of glutamic acid, 0.2-0.75 part of betaine, 1-2 parts of vitamins and 1-3 parts of Chinese herbal medicines. The feed has advantages that growth development is promoted, stress resistance and immunity of leiocassis longirostris are improved, and symptoms including surface hyperemia, transverse inclining and mass mortality of leiocassis longirostris from the later aquaculture period to a process of transferring for marketing are avoided.

The invention relates to a Leiocassis longirostris skin collagen antioxidation peptide, and a preparation method and application thereof. The preparation method comprises the following steps: removing alkali-soluble proteins from Leiocassis longirostris skin with an alkali solution, degreasing with n-hexane, extracting collagen with a lactic acid solution, dialyzing, carrying out freeze-drying to obtain the collagen, hydrolyzing the obtained collagen with proteinase under proper enzymolysis conditions, and carrying out freeze-drying on the enzymolysis product to obtain the Leiocassis longirostris skin collagen antioxidation peptide. The Leiocassis longirostris skin collagen antioxidation peptide has favorable activity, the IC50 for removing DPPH free radicals is 0.53-0.86 mg / ml, the IC50 for removing hydroxy free radicals is 0.72-0.91 mg / ml, and the IC50 for chelating Fe<2+> is 1.01-1.35 mg / ml.

The invention discloses a method for establishing a leiocassis longirostris breeding dynamic feeding table, comprising the following steps of: 1. researching the dynamic relationship between the feeding level, the weight and the water temperature and the maximum feeding rate, the defecation rate, the excretion rate, the body heat gain, the standard metabolism and the activity metabolism in the energy budget expression through a growth experiment; 2. establishing an optimum feeding rate model of leiocassis longirostris on the basis of a bioenergetic model; and 3. inputting the breeding days, water temperature, the initial body weight of fishes, initial energy values of fish bodies, the feed protein level and the feed energy value by using a computer program, outputting the daily weight growth, the maximum daily feeding quantity and the optimum feeding quantity and forming the leiocassis longirostris breeding dynamic feeding table by calculation. The dynamic feed can accurately forecastthe daily growth and the daily feeding rate of the leiocassis longirostris in the breeding process under different environments in time, which not only cannot waste the feed to cause the cost increase and the secondary pollution of the feed, but also cannot influence the growth of fishes, caused by lack feeding of the feed.

The invention relates to a controlled-feeding / heavy-feeding alternate feeding aquatic product fish culture method. According to the method, a controlled-feeding / heavy-feeding alternate feeding mode is provided by using the principle of compensatory growth, so that the feed utilization efficiency of fishes can be increased, the wasting of feedstuff and the emission of nitrogen and phosphorus caused by remnant feedstuff are reduced, the environmental pollution is lightened, and the culture cost is reduced. By a controlled-feeding / heavy-feeding alternate feeding strategy, the feed utilization efficiency of fish bodies of sturgeons or Leiocassis longirostris can be increased, and the emission of nitrogen is lowered; and the content of protein and fat in the fish bodies is not affected, the fat accumulation of internal organs can be lowered, and the quality is improved.

A Leiocassis longirostris intensive artificial propagation method, comprises parent fish selection, induced spawning, hatching. According to the present invention, individuals of Leiocassis longirostris whose bodies weigh more than 2.5 kilogram and body surfaces are smooth as parents, and the Leiocassis longirostris parents are disease-free and injurt-free, healthy, and appear sexually mature are selected. The ratio of female fishes and male fishes is 1:1 to 1:1.5. The induced spawning includes the installation of a specific oviposition pool and an oviposition-nest, preparation of oxytocic in different doses, and spawn taking. The hatching includes the determination of a hatching groove and the hatching condition. The method of the present invention saves manpower, material resources and financial resources, and can produce fry by bulk according to a plan in order to satisfy the requirements for fry of different customers, makes production and development be unified; and avoids severe destruction of nature ecology caused by killing the male parents in a large number.

The invention discloses a special feed capable of increasing the survival rate of leiocassis longirostris. The special feed comprises the following raw materials in parts by weight: 15-30 parts of sweet sorghum, 20-40 parts of oat flour, 10-20 parts of alfalfa powder, 10-18 parts of water hyacinth dry powder, 60-70 parts of a vegetable protein additive, 4-12 parts of kelp powder, 4-10 parts of puffed blood powder, 2-4 parts of rice wine distillers' grains, 2-4 parts of yeast protein, 4-8 parts of an immune enhancer, 2-4 parts of a Chinese herbal medicine additive and 4-6 parts of a chitosan compound, wherein the chitosan compound is prepared through the following technology: stirring the bentonite, the chitosan, a silane coupling agent and water, performing ultrasonic vibration, adding Tween 80, continuing performing stirring, performing filtering, performing washing and performing drying, so that the chitosan compound is obtained. According to the special feed, the leiocassis longirostris can easily adapt to breeding environment, the survival rate of seedlings is high, the immunity is high, the obtained leiocassis longirostris has the characteristics of being natural in body colorand healthy in fish body, the nutrient value of the leiocassis longirostris bred with the feed disclosed by the invention can be the same as that of wild leiocassis longirostris, and the requirementsfor high nutrient value of consumers can be met.

The invention relates to instant soft-packaged leiocassis longirostris and a preparation method thereof. The instant soft-packaged leiocassis longirostris is prepared by pickling 1.5-2.0 kg of pretreated fresh leiocassis longirostris meat pieces by adopting pickling seasoning, wherein the pickling seasoning comprises the following components in percentage by weight: 6-10% of white granulated sugar, 3-5% of table salt, 1-2% of monosodium glutamate, 8-10% of soy sauce, 2-4% of Chinese prickly ash powder, 1-2% of five spice powder, and 30-50% of fresh chillies, then performing drying under the condition of the hot air drying temperature of 50-90 DEG C for 3-8 h, and finally carrying out vacuum packaging and high-temperature sterilization. The invention also discloses the preparation method of the instant soft-packaged leiocassis longirostris. The products provided by the invention are thick in meat quality, moderate in spicy and hot flavor, nutritive and safe, and can be used as instant leisure products to be put on the market.

The invention belongs to the technical field of food processing, and in particular relates to leiocassis longirostris-containing lentinus edods sauce. The leiocassis longirostris-containing lentinus edods sauce is prepared from main materials and auxiliary materials, wherein the main materials comprise leiocassis longirostris meat, fermented black beans and dry lentinus edods; the auxiliary materials comprise main spices, auxiliary spices, vegetable oil, seasonings, lemongrass herb powder, fructus amomi rotundus powder, spiceleaf powder, fructus foeniculi powder, radix angelicae sinensis, radix codonopsis, radix panacis quinquefolii, radix adenophorae and white sesame; the leiocassis longirostris-containing lentinus edods sauce is prepared by means of a special sauce cooking technology. The lentinus edods sauce prepared by combining the lentinus edods with leiocassis longirostris has double flavors of the lentinus edods and the leiocassis longirostris; due to the combination of the lentinus edods and the leiocassis longirostris, not only is the umami of the traditional lentinus edods sauce improved, but the nutritional value of the traditional lentinus edods sauce is also increased; furthermore, the leiocassis longirostris-containing lentinus edods sauce can enhance the immunity of a human body, reduce the blood pressure and reduce the content of cholesterol.

The invention discloses an environmental-friendly growth-promoting feed for Leiocassis longirostris of 60 to 120 days old. The environmental-friendly growth-promoting feed comprises, by weight, 10 to 30 parts of soybean meal, 5 to 15 parts of silkworm chrysalis meal, 10 to 20 parts of shrimp meal, 5 to 15 parts of peanut meal, 5 to 10 parts of scallop skirt, 5 to 15 parts of undaria pinnatifida powder, 2 to 10 parts of full fat rice bran, 10 to 30 parts of shell powder, 1 to 5 parts of diatomite, 5 to 15 parts of sargassum, 10 to 18 parts of rice flour, 15 to 30 parts of Sargassum pallidum, 1 to 2 parts of yeast polypeptide powder, 0.5 to 1.5 parts of fructooligosaccharide, 1 to 3 parts of choline chloride, 1 to 3 parts of monocalcium phosphate, 1 to 2 parts of composite vitamin, 0.2 to 0.8 part of high temperature resistant phytase, 1 to 5 parts of modified corn protein powder, and 1 to 3 parts of a Chinese herbal medicine. The environmental-friendly growth-promoting feed is capable of satisfying nutrient demands of Leiocassis longirostris of 60 to 120 days old, promoting rapid growth, improving immunity and anti-stress capability, and reducing aquatic water environment pollution.

The invention discloses green and healthy leiocassis longirostris feed. The feed includes the following ingredients in parts by weight: 30-50 parts of brown rice flour, 15-25 parts of sunflower seed meal, 10-18 parts of radish leaves, 4-15 parts of potato flour, 2-8 parts of oat bran, 4-12 parts of peanut meal, 1-3 parts of pollen pini, 2-5 parts of whole wheat flour, 2-4 parts of corn gluten meal, 5-10 parts of rice chaff, 4-8 parts of rice bran meal, 2-6 parts of a hyaluronic acid compound, 2-4 parts of an intestinal probiotic preparation and 2.5-4.5 parts of a digestion-promoting agent. The feed provided by the invention has a reasonable combination of various nutrients, can not only save feed but also decrease the addition amount of chemicals and feeding cost, improve benefits of feeding the leiocassis longirostris, improve feed nutrient structure and enhance immunity. According to the invention, the ingredients of the feed are natural and hormone-free and meet the current demand of people on food safety. The feed provided by the invention can facilitate the growth of the leiocassis longirostris and increase yield and economic benefits.

The invention belongs to the technical field of food processing, and particularly relates to a production method of dumpling with fish meat stuff by taking fish meat as a main raw material. The method comprises the following steps: removing skin and bones of leiocassis longirostris, washing pork cleanly and cutting the cleaned pork into blocks, mincing for later use through a meat mincer, feeding meat, vegetables and other raw materials and auxiliaries into a stirring machine and beating into stuffing, forming dumplings through a dumpling forming machine or manually, arranging the formed products into an instant freezer for quick freezing, bagging, weighing and sealing according to the product specification requirement, and timely delivering entirely packaged products into a finished product freezer. The produced dumplings are attractive in appearances, are fragrant and tender, good in mouth feeling, and rich in nutrients, the dumplings produced by adopting the method according to the proportion not only are delicious in taste and excellent in mouth feeling, and has the effects of strengthening the physique, improving the immunity, delaying senescence and the like after being eaten for a long time, and for the dumplings produced by adopting the method, the stuffing cannot be broken and loosened and cannot suffer from other conditions during cooking, the delicious mouth feeling of the dumplings can be kept, and therefore, the dumplings have wide market prospects, and are beneficial to popularization and applications in the field.

A production method of a shiitake and Leiocassis longirostris sauce comprises the following steps: 1) cleaning Leiocassis longirostris meat, cutting the Leiocassis longirostris meat to form blocks, adding a fish seasoning solution, and uniformly mixing the blocks and the solution; 2) sending the seasoned Leiocassis longirostris meat to a tumbling machine, and tumbling the seasoned Leiocassis longirostris meat; 3) placing the tumbled Leiocassis longirostris meat in a refrigerating cabinet, refrigerating the Leiocassis longirostris meat, placing the refrigerated Leiocassis longirostris meat in a heating tank, and sealing the heating tank; 4) sending the heated Leiocassis longirostris meat to a refrigerating bed; 5) taking out the refrigerated Leiocassis longirostris meat, unfreezing the Leiocassis longirostris meat in water, draining the Leiocassis longirostris meat, placing the Leiocassis longirostris meat in a pot, and frying the Leiocassis longirostris meat in oil until the Leiocassis longirostris meat is golden and crisp; 6) soaking dry shiitake, cutting the soaked shiitake to form dices, blanching the dices, airing the blanched dices, and refrigerating the aired dices; 7) placing the refrigerated shiitake dices in a cold air drying device, and drying the shiitake dices; 8) placing the dried shiitake in the pot, frying the dried shiitake in oil until the shiitake is crisp, and crushing the fried shiitake to form powder; 9) placing the fried Leiocassis longirostris meat in the pot, adding the shiitake powder, soybean sauce and high fructose corn syrup, uniformly stirring above materials, adding seasoning auxiliary materials, boiling materials, carrying out heat insulation for 5 min, and transferring the boiling materials to a stainless steel container; and 10) fully dissolving modified starch in water in a burdening barrel, pouring the obtained modified starch solution to the container, cooling the materials and the solution, loading the obtained mixture, and sterilizing the mixture to obtain the shiitake and Leiocassis longirostris sauce.

The invention discloses a feed for Leiocassis longirostris in the fattening period. Raw materials of the feed comprise 30-50 parts of fermented grape seed meal, 20-40 parts of fermented sunflower seed meal, 5-10 parts of peanut meal, 2-10 parts of fish meal, 2-5 parts of honey, 1-2 parts of egg white, 4-12 parts of phoenix eggs, 1-4 parts of kelp powder, 1-2 parts of Herb of Hairystalk Loosestrife, 4-8 parts of an antibacterial system, 8-12 parts of a granulation system, 0.15-0.35 parts of fructooligosaccharides, 0.1-0.6 parts of nicotinamide and 0.01-0.04 parts of EM bacteria. A corn flour compound is prepared through the following steps: stirring corn flour, hydroxypropyl guar gum and an ethanol solution, adding 2-chloroethyldiethylamine, stirring above substances, filtering the stirred substances, drying the filtered substances until the weight is constant, and crushing the dried substances. The feed has the advantages of easily available raw materials, low cost, richness in nutrients, good dispersibility, easiness in digestion and absorption by the Leiocassis longirostris, effective reduction of the mortality rate of the Leiocassis longirostris, difficulty in dissolving of a bait in water, and facilitation of a water environment.

The invention discloses a novel Leiocassis longirostris breeding system capable of automatically adjusting feeding amount. The system comprises a collecting unit, a storing unit, a feeding unit and a control unit, wherein the collecting unit is used for collecting the integral infrared image information of the interior of a fishpond; the storing unit is used for storing a corresponding list of the fish volume sum V and the feeding amount sum W; based on an instruction of the control unit, the targeted feeding amount of food is fed into the fishpond by the feeding unit; the integral infrared image information of the interior of the fishpond is acquired by the control unit at t<1>, t<2>...t<n>, the fish volume sum V<1>, V<2>...V<n> in the fishpond is calculated based on the infrared image information, when V <= a*V<i-1>, the control unit sends W<i-1> as the targeted feeding amount to the feeding unit and controls the feeding unit to take action through the instruction, and when a*V<i-1> < V and V <= b* V<i-1>, the feeding amount sum W corresponding to V is searched in the storing unit by the control unit, and the control unit sends W to the feeding unit and controls the feeding unit to take action through the instruction. According to the system, a feeding strategy is selected and adjusted based on changes of the fish volume in a targeted mode, intelligentization and automation of the fish breeding system are improved, and the effect and efficiency of fish breeding are guaranteed.

The invention provides a pollution-free sterilization type Leiocassis longirostris culture method. The pollution-free sterilization type Leiocassis longirostris culture method includes establishing a stereoscopic aquaculture farm and a sterilization system; establishing a recirculating aquaculture system in a bottom layer, namely arranging aquaculture wastewater purifying ecological ditches, a wastewater sedimentation pond and a purifying pond in the bottom layer; feeding Leiocassis longirostris on the bottom layer with forage fishes or aquatic insects cultured on the top layer, wherein a feeding proportion is controlled according to feeding dates and aquaculture water source temperatures; conducting daily management by selecting fresh water injection time and oxygenation time reasonably; conducting disease control. The pollution-free sterilization type Leiocassis longirostris culture method has the advantages that the stereoscopic aquaculture farm and the sterilization system are established, and aquaculture water is sterilized through a physical method, so that pollution of chemicals to the aquaculture water and secondary pollution caused by chemical intake of the Leiocassis longirostris are avoided; since the forage fishes or aquatic insects for feeding the Leiocassis longirostris are cultured with the Leiocassis longirostris, ecological environmental protection is achieved, and aquaculture efficiency is improved; the recirculating aquaculture system is used for recycling the aquaculture water source, and accordingly aquaculture cost is reduced.

The invention provides a method for processing instant leiocassis longirostris fins, relating to the technical field of food processing. The method comprises the following process flows: washing leiocassis longirostris fins, removing the impurities such as fish blood and fish intestines and draining the leiocassis longirostris fins for later use; salting the leiocassis longirostris fins, specifically, weighing the treated leiocassis longirostris fins according to a weight ratio of the leiocassis longirostris fins to the marinade being 100 to 8, mixing the leiocassis longirostris fins with the marinade uniformly and salting the leiocassis longirostris fins in a cold storage at 0-4 DEG C for 6 hours; then draining the surplus marinade on the leiocassis longirostris fins, putting the leiocassis longirostris fins in a vacuum low-temperature cold air dryer and maintaining the leiocassis longirostris fins at 40-50 DEG C for 60-90 minutes; carrying out vacuum packaging on the dried leiocassis longirostris fins with aluminium foil bags; sterilizing the packaged leiocassis longirostris fins at 121 DEG C for 20-30 minutes and then cooling and coding the leiocassis longirostris fins, thus obtaining the instant leiocassis longirostris fins. The method is low in raw material cost and simple in process, is suitable for industrial production, has the effect of greatly increasing the additional values of fish byproducts and has good market prospect.

An instant leiocassis longirostris head eaten with wine is prepared from leiocassis longirostris head and spices according to 100:8, wherein the spices consist of the following components by weight: 6 kg of salt, 3.5 kg of white granulated sugar, 1.5 kg of paprika powder, 1.5 kg of chilli powder, 0.3 kg of cumin powder, 0.2 kg of fresh ginger powder, 0.3 kg of garlic powder, 0.05 kg of clove powder, 0.08 kg of caraway seed powder, 0.02 kg of spicy essence, 0.4 kg of gourmet powder, 0.5 kg of white spirit, 0.5 kg of oyster juice, 0.2 kg of honey, 0.25 kg of nutritional seasoner, 0.05 kg of whole milk powder, 0.05 kg of a peach blossom extract, 0.05 kg of a lily extract, 0.05 kg of a pepper leaf extract, 0.05 kg of corn peptide and 0.05 kg of wheat oligopeptide.

The invention provides a primer and a method for molecular identification of channel catfish and leiocassis longirostris. The nucleotide sequence of the primer is shown as SEQ NO: 1 and SEQ NO: 2 or SEQ NO: 3 and SEQ NO: 4. Similar fishes such as channel catfish are identified from the cytochrome oxidase subunit I gene and the cytochrome b gene, and the two fishes can be rapidly, conveniently, accurately and reliably identified by utilizing the primers through a mitochondrial genome DNA bar code technology; the primer and the method can quickly, conveniently, accurately and reliably identify channel catfish, ietalurus punetaus and the like, are good in result stability and high in repetition rate, fill the blank of identifying channel catalurus punetaus, ietalurus punetaus and pelteobagrus fulvidraco according to domestic molecular biological standards at present, play an important role in ecological resource investigation and fish variety identification, and have good application prospects. Wide application prospects are realized.

The invention discloses special puffed compound feed for leiocassis longirostris and a preparation method thereof. The feed is prepared from the following ingredients in parts by weight: 25 to 28 parts of fish meal, 10 to 15 parts of fish steak powder, 2 to 3 parts of spray-dried animal blood cells, 30 to 35 parts of bean pulp, 10 to 15 parts of rapeseed meal, 10 to 15 parts of high gluten flour,2 to 3 parts of bean oil, 4 to 5 parts of bentonite, 2 to 3 parts of mountain flour, 1 to 2 parts of monocalcium phosphate, 0.1 to 0.5 part of choline chloride, 0.1 to 0.5 part of mildew preventives,0.01 to 0.05 part of antioxidants and 0.1 to 0.3 part of premixed materials. All ingredients are mixed and are then crushed to 60 to 80 meshes; next, expansion and granulation are performed. The special puffed compound feed can meet the growth requirements of the leiocassis longirostris; the growth speed of the leiocassis longirostris is accelerated; the feed conversion efficiency is improved.

The invention discloses a special starter feed for juvenile leiocassis longirostris and a preparation method thereof. The method provides a comprehensive and sufficient supplement for the organ development of juvenile fish by selecting natural animal meat bait; at the same time, the natural meat bait is a good attractant and very effective in promoting the early feeding of juvenile leiocassis longirostris; the meat material is more conducive to the digestion and absorption of juvenile fish because of beating treatment; by plant additives, the antioxidant and mildew-proof effects of feed can beachieved, the side effects of synthetic antioxidants and mildew-proof agents on juvenile leiocassis longirostris are avoided, and at the same time, harmful bacteria can also be inhibited.

The invention provides an SNP marker related to the growth rate of leiocassis longirostris and application of the SNP marker. The SNP marker comprises an SNP1 marker and an SNP2 marker, and nucleotide sequences of the SNP1 marker and the SNP2 marker are shown as SEQ ID NO.1 and SEQ ID NO.2 in sequence. When the genotype of the SNP1 site is mutated homozygous TT and the genotype of the SNP2 site is mutated homozygous GG, the growth speed of the individual leiocassis longirostris to be detected is higher. The individuals with the genotype of TT at the SNP1 site and the individuals with the genotype of GG at the SNP2 site are selected to be hybridized, and the offspring individuals are of a rapid growth type. The SNP marker disclosed by the invention is closely related to the growth speed of the leiocassis longirostris, parents can be selected according to breeding requirements in the early stage of fry culture, and the marker disclosed by the invention is used for assisted breeding, so that the culture process of good varieties of the leiocassis longirostris is accelerated.

The invention discloses a safe and environment-friendly feed for leiocassis longirostris culturing. Raw materials of the feed comprise, in parts by weight, 15-25 parts of peanut powder, 18-26 parts of puffed sweet potato powder, 8-14 parts of chironomid larvae powder, 10-20 parts of fermented peanut meal, 2-6 parts of soya-bean milk powder, 1-8 parts of sunflower seed meal, 4-10 parts of dry common waterhyacinth herb powder, 1-4 parts of Chinese wolfberry leaves, 1-5 parts of beer yeast, 0.4-1.2 parts of lecithin, 0.5-1.5 parts of cassia seed powder, 1-2 parts of silage straw, 3.5-7 parts of a digestive agent, and 2.5-6 parts of an immunopotentiator. The feed is comprehensive in nutrients and balanced in proportioning, facilitates the digestion and absorption of the leiocassis longirostris, enables high growth conversion efficiency, and promotes the rapid growth of the leiocassis longirostris. Moreover, the feed contains no chemical reagent, so that fish flesh quality is guaranteed, and the fish flesh is safe and environmentally friendly so as to meet present demands of people on food safety. Meanwhile, excreta contains a small amount of organic residues so as to effectively maintain microbe environment balance in leiocassis longirostris intestinal tracts, avoid the generation of intestinal tract diseases, and realize good vitality of leiocassis longirostris bodies.