216 results about "Hybrid species" patented technology

A method of isolating genomic or RNA-derived duplex fragments which are unique to one of two fragment mixtures. The fragments in positive-source and negative-source mixtures are separately equipped with end linkers, and each mixture is amplified by successive primed-strand replications, using a single primer which is homologous to the associated linker. The second-source linker is biotinylated, and the fragments in this mixture are hybridized in molar excess with the fragments in the positive-source mixture. DNA species which are not hybridized with the biotinylated species, i.e., species that are unique to the positive-source mixture, are isolated after removal of hybridized species by affinity chromatography. Also disclosed is a method of amplifying a mixture of DNA fragments by repeated linker/primer replication.

The present invention provides a method of herbicide treatment of selected female and male parents in a hybrid seed production system. The system results in enhanced trait purity and efficiency in the hybrid seed production system.

A method for plant breeding by identifying, balancing, and combining multiple traits is provided. The method enhances the profitability of a hybrid corn seed production system by providing traits having high impact on decreasing cost of production on female parent and traits having low impact on decreasing cost of production and end users traits on the female and/or the male parent. The invention also provides a hybrid corn seed produced by such method.

A process is described for producing hybrid seed using male-sterile plants created by employing molecular techniques to manipulate antisense DNA and other genes that are capable of controlling the production of fertile pollen in plants. Transformation techniques are used to introduce constructs containing antisense DNA and other genes into plants. Said plants are functionally male-sterile and are useful for the production of hybrid seed by the crossing of said male-sterile plants with pollen from male-fertile plants. Hybrid seed production is simplified and improved by this invention and can be extended to plant crop species for which commercially acceptable hybrid seed production methods are not currently available.

The invention relates to a method for high-efficiently cultivating excellent later generation of the wild rice distant hybridization , which is to cultivate the wide rice distant-hybridization high-efficient excellent later generation through the hybridization of the cultivated rice, the embryo rescue, the strong seedling culture, the pollen culture and the molecular marker assisted selection of the hybridization later generation of the cultivated rice and the wide rice. The survival rate of the distant hybridization of the invention is high; the hybrid progeny is backcrossed at the preliminary period and then self-bred, thus being beneficial to overcome the reproductive barrier caused by the different genome in the hybrid progeny of the wide rice and the cultivated rice, and to stabilize more genetic characters of the wide rice inside the cultivated rice; the pollen culture can accelerate the stability of the purification of the hybrid progeny; with the established molecular marker assisted selection, the breeding can be accurately performed without being influenced by the environmental conditions and being restricted by the growth and development period.

The invention relates to a method for rescuing blast to obtain remote hybrid of lily, wherein it selects YunNan wide lily and better system, uses lily hybrid as male, to cross; uses cross pollination and blast separation cultivation to take it out and cultivate before fail, to avoid earl decay, to grow remote hybrid blast into normal seed, to shorten the select period and improve survival rate.

According to the invention, there is provided seed and plants of the corn variety designated I286394. The invention thus relates to the plants, seeds and tissue cultures of the variety I286394, and to methods for producing a corn plant produced by crossing a corn plant of variety I286394 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety I286394 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety I286394.

A method for culturing two hybrid varieties of fish includes such steps as providing wuchangfish (or red-scale carp) as female parent and topmouth culter (or red crucian carp) as male parent, injecting oxytocic, taking the mature ova, artificial insemination, incubation, culturing black fry by feeding soybean milk for 10-14 days, and culturing for 4-6 months. Its advantages are unique shape, high growth speed, and high resistance to stress.

The invention discloses a culture method for triploid grape culture by embryo rescue and early identification of ploidy. The invention aims to cultivate triploid grape by embryo rescue and overcome the problem of seed abortion of hybrid embryo between diploid and tetraploid grape cultivars in conventional breeding. After a hybrid seed is obtained, chromosome count is performed by the combination of flow cytometry and wall degradation hypotonic method to identify the ploidy of the hybrid progeny. The invention increases the breeding efficiency of triploid grape, accelerates the breed selection of triploid grape variety and provides a new approach to the culture of seedless grape variety.

The invention provides seed and plants of spinach hybrid RX 06692157 and the parent lines thereof. The invention thus relates to the plants, seeds and tissue cultures of spinach hybrid RX 06692157 and the parent lines thereof, and to methods for producing a spinach plant produced by crossing such plants with themselves or with another spinach plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the leaf and gametes of such plants.

A Method for producing triploid corn seeds and plants is described. The method comprises combining two parent inbreds of different ploidy levels, wherein one parent inbred is a tetraploid (4N) and the other parent is a diploid (2N) so as to produce a triploid hybrid corn seed; and cultivating the triploid hybrid corn seed to form a triploid corn plant. Usage of triploid corn plants as an economic source of sugar and ethanol is described, as is the production of molasses, rum and fodder from plant material of the low sterility triploid corn plants.

A method for selectively culturing the allogenic cytoplasm male sterility line of rape includes such steps as separating the protoplast from leaf pulp, purifying, pre-treating, fusion, culturing, discriminating hybrid, reproduction, and continuous backcross to obtain sterility line and maintenance line.

The invention discloses a cDNA sequence represented by the SEQ ID No.1 of a paddy rice leaf color control gene OscpSRP54 coding area and an amino acid sequence represented by SEQ ID No.2 of a protein encoded by the gene. The gene OscpSRP54 which controls the paddy rice leaf color is cloned from a paddy rice light green leaf mutant HM14 through a map-based cloning technology, and the gene OscpSRP54 is proved to be the gene that control the paddy rice leaf color through a functional complementation experiment. Through observation of chloroplast by using a transmission electron microscope, the fact that the OscpSRP54 gene has the function of modulating chloroplast growth and then further controlling the leaf color is proved. The OscpSRP54 gene can be used to modulate paddy rice chloroplast growth so as to improve the photosynthesis efficiency and increase the paddy rice output. The leaf color control gene provided by the invention can also be used as a tracing marker in a genetic transferred plant progeny or an indicating marker for distinguishing pure species from hybrid species in a hybrid seed production process, and can be applied to the fields of good species breeding and hybrid breeding.

The invention provides a primer group for identifying a male parent and a female parent of tachysurus fulvidraco and leiocassis longirostris hybrid species. The primer group comprises a primer pair for amplifying COI genes and a primer pair for amplifying an ITS sequence, wherein the primer pair for amplifying COI genes consists of a primer COIF and a primer COIR; the primer pair for amplifying an ITS sequence consists of a primer ITSF and a primer ITSR. The invention also provides a kit which is for identifying the male parent and the female parent of tachysurus fulvidraco and leiocassis longirostris hybrid species and comprises the primer group. The kit also comprises any one or more of the following reagents: a buffer solution for PCR, dNTP, heat-resistant DNA polymerase, a cloning vector, a DNA ligase, and a buffer solution for ligation. The invention also provides a method for identifying the male parent and the female parent of tachysurus fulvidraco and leiocassis longirostris hybrid species. The primer group, the kit or the method can be used for accurately identifying the male parent and the female parent of the tachysurus fulvidraco and leiocassis longirostris hybrid species, thus effectively managing a breeding plan and monitoring negative influence thereof, and has great significance in sustainable development of aquaculture industry.

The present invention provides tobacco inbred plants ALBEX1F and ALBEX1MS. The present invention also provides parts of such plants and products made from those parts. The present invention also includes progeny of the provided plants including hybrids.

The invention provides a method for cultivating hybrid plants of far-source lilies, which overcomes the problem that far-source hybrids are not easy to be affinitive in the prior art. The method performs in vitro culture to an ovary and an anther, adopts an unconventional pollination mode of cutting off the ovary for in vitro pollination, and then performs embryo culture so as to obtain a hybrid plant. Hybrid seedlings are obtained by use of the method, which proves that the method successfully overcomes the problem that the far-source hybrids are not affinitive, can well improve seed setting rate, and is worthy to be popularized and applied.

The invention provides a cultivating method of a new species of an anti-late blight and anti-beetle potato, relating to a cultivating method of a new species of the potato with a cell culture technology. The method comprises the following steps of: electrically fusing a protoplast (A) which has anti-late blight gene, anti-beetle gene and anti-oxidation gene, and is separated from the wild species of the potato, with a protoplast (B) of the potato culturing species with wide adaptability to form into a fusion cell with the protoplast A and the protoplast B; generating a callus by means of cell culture, and generating a hybrid test tube plantlet of a regenerated somatic cell by means of induction; and further culturing the tissue culture test tube plantlet, transplanting the cultured tissue culture test tube plantlet into a glasshouse or a land for growing field crops, and generating a test tube first generation somatic cell hybridized potato to obtain the anti-diseases and insect cell hybrid potato, wherein the somatic cell hybridized potato has the good agronomic trait of the potato cultivating species and the anti-late blight and anti-beetle trait. The cultivating method provides a new available gene resource which can be used for improving the quality of the potato and improving the antioxidant activity of the potato cultivating species with the wild species.

A novel cotton variety, designated as FM 989B2R, is disclosed. The invention relates to seeds, plants, plant cells, plant tissue, harvested products and cotton lint as well as to hybrid cotton plants and seeds obtained by crossing plants of variety FM 989B2R with other plants. The invention also relates to plants and varieties produced by the method of essential derivation from plants of FM 989B2R and to plants of FM 989B2R reproduced by vegetative methods, including but not limited to tissue culture of regenerable cells or tissue from FM 989B2R.

The method for cultivating approximate hybrid species by utilizing anther of hot-pepper hybrid species includes the following steps: 1. sowing hot-pepper hybrid seed, in bud stage of plant taking budand making anther culture; 2. utilizing anther culture to obtain haploid plant, and utilizing chromosome doubling to obtain double haploid line of original hot-pepper hybrid species; 3. in several double haploid lines, according to main economic trait and approximate property of original hybrid species, selecting parental line which can be used for original hot-pepepr hybrid species and breaking several hot peper hybrid species; and 4. comparing, testing and breeding approximate hybrid species which has no clear difference from original hot-pepper hybrid species in main economic trait.

The invention discloses a breeding method of wavy Brassica oleracea var. acephala f. tricolor Hort., which comprises the following steps: hybridizing by using asparagus broccoli cytoplast male sterile material CMS034 as a female parent and red curly kale inbred line Ruiyu 6 as a male parent; backcrossing with the original male parent by selecting the individual plant, which has characters closest to the parental characters, from filial generation as the female parent; repeating the second step, and continuously backcrossing to obtain a red curly kale male sterile line; and hybridizing to obtain the wavy Brassica oleracea var. acephala f. tricolor Hort. hybrid by using the red curly kale male sterile line as the female parent and amaranth broad-leaf Brassica oleracea var. acephala f. tricolor Hort inbred line Y305 as the male parent. The new hybrid species obtained by the method disclosed by the invention has the advantages of high ornamental value, long ornamental period, cold resistance and disease resistance.