182 results about "Cytoplast" patented technology

Chimeric receptor genes suitable for endowing lymphocytes with antibody-type specificity include a first gene segment encoding a single-chain Fv domain of a specific antibody and a second gene segment encoding all or part of the transmembrane and cytoplasmic domains, and optionally the extracellular domain, of an immune cell-triggering molecule. The chimeric receptor gene, when transfected to immune cells, expresses the antibody-recognition site and the immune cell-triggering moiety into one continuous chain. The transformed lymphocytes are useful in therapeutic treatment methods.

The present invention includes nuclear fertility restorer genes, proteins encoded by those genes and transgenic plants and plant cells containing those genes. More particularly, the nuclear fertility restorer genes can be used to restore fertility in cytoplasmic male-sterile plants such as Brassica napus. Preferably, the nuclear fertility restorer genes are used with the Ogura (ogu) CMS system in Brassica napus.

The invention discloses functional albumin and a preparation method of a nano preparation of the functional albumin. The nano preparation of the functional albumin consists of the functional albumin, metal ions and a drug; the metal ions can simultaneously form coordination bonds with the functional albumin and the drug, and can form nanoparticles through induced self-assembly. The nano preparation, through an endocytosis mediated by an albumin receptor (SPARC) on the surface of tumor cells, can deliver the drug into the drug-resistant tumor cells, so as to effectively avoid the exocytosis effect of a p-gp pump on the drug, and then as the coordination bonds break in an acid tumor cell environment through a pH responsibility, the drug releases in cytoplast, enters cell nucleus and inlays in DNA so as to inhibit the synthesis of nucleic acid; and therefore, the growth of the tumor cells is inhibited. Through in vitro characterization, the nano preparation can achieve the relatively good pH responsibility; and through activity evaluation on a cellular level, the system is capable of effectively delivering the drug into the cells, so as to achieve relatively good pH responsive release.

The invention relates to a breeding method and application of a cytoplasmic male sterility restoring line of brassica napus rapeseed and radish. The method comprises the following steps of: choosing rapeseed S1628 with the preservation number of CCTCC NO: P201203, performing continuous inbreeding directional selection for six years to breed an excellent, high-oil and stable inbred line; using the cytoplasmic male sterility restoring line of the brassica napus rapeseed and radish as a female parent and using the bred excellent and stable inbred line as a male parent to select out strains comprising radish cytoplasmic male sterility restoring genes from large amounts of filial generations; using the strains comprising the radish cytoplasmic male sterility restoring genes as the female parent and using Zhongshuang No.2, Huashuang No.3, Huyou 17, Zunyou No.1 and Zheyou 758 of double-low winter rapeseed varieties in China as recurrent male parents to hybridize, performing the continuous inbreeding directional selection for six years to breed the cytoplasmic male sterility restoring line of the double-low and high-oil brassica napus rapeseed and radish. The restoring line has been crossed with hybrid varieties. A gap of producing the hybrid varieties by the cytoplasmic male sterility restoring line of the double-low radish in China is filled, and the application prospect is broad.

The invention relates to the field of accessories and preparations with medicinal functions, in particular to a kind of oligopeptide-based pH-sensitive amphoteric ion lipid (I) or (II). In the invention, the derivatives of the oligopeptide amphoteric ion lipid have different surface charges under different pH conditions and can take relatively strong negative charges in a physiological environment with pH near neutrality so that the lipid has good blood compatibility; when the lipid reaches a tumor part, the surface charges of the oligopeptide amphoteric ion lipid are reversed in an acidulous tumor environment, so that the original electronegativity is reversed into electropositivity; and a carrier with positive charges is easily combined with the surface of a tumor cell, and the tumor cell targeting can be effectively realized through an endocytosis path. In lysosome, a cationic carrier continuously realizes a function of proton sponge or a function of splitting lysosome membranes, and the two functions can ensure the integrity of the carrier, so that the carrier can safely escape to cytoplast or other organelles.

This disclosure concerns high-resolution mapping and candidate gene cloning of Rf4, a maize restorer of fertility gene that restores fertility to C-type cytoplasmic male sterility. The disclosure also relates to molecular markers that are tightly-linked to, or reside within, the Rf4 gene. In some embodiments, methods are provided whereby hybrid seeds may be produced from crosses of a male plant comprising nucleic acid molecular markers that are linked to or that reside within the Rf4 gene and a female plant carrying C-type CMS.

The invention relates to a method of making pluripotent stem cells that does not involve the formation of early preimplantation embryos or fetal tissue. The method has general utility in the production of pluripotent stem cells from many mammalian species but has particular application in man where pluripotent stem cell production can be customized to particular human individual. The method involves the fusion of donor somatic or stem cells (or their karyoplasts) with cytoplasmic, membrane-delimited fragments of mammalian oocytes or zygotes. After the initial genomic reprogramming occurs, the cells can proliferate and thus multiply in vitro yielding a large number of autologous cells for cell therapy application. The result of this process is a cell population genomically identical to the somatic, differentiated cells derived from an individual patient. However, these cells are pluripotent in that upon application of specific growth factors, the cells are capable of differentiating into specific cell types as required by the sought clinical indication.

This invention provides a nucleus transplantation method for mammal somatic cells characterizing in taking the oocyte of cross-fertilized F1 generation of the mammal as the recipient somatic cell for nucleus transplantation, which can increase the re-programming ability of recipient cytoplast of nucleus transplantation of mammals obviously, improve the growth of re-structured embryo to increase the clone efficiency of somatic cells.

The invention belongs to the technical field of molecular biology and discloses a pepper sterility restoring gene closely linked molecular marker, a method and application. The molecular marker is scd06-17 and comprises a nucleotide sequence shown as SEQ ID NO.1 and a nucleotide sequence shown as SEQ ID NO.2. The molecular marker is stable and reliable in result and is a co-dominant molecular marker, whether a pepper material contains a cytoplasmic male sterility restoring gene or not can be determined in a pepper steeling stage, and accordingly breeding efficiency is improved, and a breeding process is accelerated. Compared with other domestic and international published pepper restoring gene molecular markers, the pepper sterility restoring gene closely linked molecular marker is significant to pepper three-line hybrid seed production and restoring material molecular marker assisted selection breeding.

The invention discloses a breeding method of wavy Brassica oleracea var. acephala f. tricolor Hort., which comprises the following steps: hybridizing by using asparagus broccoli cytoplast male sterile material CMS034 as a female parent and red curly kale inbred line Ruiyu 6 as a male parent; backcrossing with the original male parent by selecting the individual plant, which has characters closest to the parental characters, from filial generation as the female parent; repeating the second step, and continuously backcrossing to obtain a red curly kale male sterile line; and hybridizing to obtain the wavy Brassica oleracea var. acephala f. tricolor Hort. hybrid by using the red curly kale male sterile line as the female parent and amaranth broad-leaf Brassica oleracea var. acephala f. tricolor Hort inbred line Y305 as the male parent. The new hybrid species obtained by the method disclosed by the invention has the advantages of high ornamental value, long ornamental period, cold resistance and disease resistance.

The invention relates to a method for transforming a sterile line of Chinese kale by utilizing vernalization and generation adding. The method comprises the following steps of: carrying out hybridization by taking a male sterile line of broccoli cytoplast as a female parent and a selfing line of the Chinese kale as a male parent, so as to obtain hybridized seeds; after the hybridized seeds are planted in summer, carrying out vernalization on plants, and meanwhile, selecting the sterile plants by utilizing pollen microscopic examination or single-plant bagging selfing; and taking the selfing line of the Chinese kale as a recurrent parent, carrying out 3-5-generation backcross with the recurrent parent and the sterile plants, so as to obtain the male sterile line of Chinese kale cytoplast. According to the method, the sterile line of the Chinese kale is transformed by utilizing the male sterile line of broccoli cytoplast, biological characters of the sterile line of the Chinese kale are stable, stamens degenerate, petals and pistils develop normally, nectaries develop well, and seed pods develop normally; the sterile line can be subjected to generation adding propagation under a normal growth condition of the plants, the transformation process is accelerated, the procedures are simplified, the cost is saved, and the purity of the Chinese kale can be improved.

The Chinese cabbage hybrid breeding process features that the process includes interspecific crossing with cabbage type rape radish cytoplast male sterile material RC97-1 as female parent and Chinese cabbage self-bred line 1138 as male parent to obtain transfer bred primary generation seed, selecting the excellent single plant as the backcross female parent for the next turn; continuous seven-generation backcross transfer breeding, economic character selection, disease resistance identification to obtain Chinese cabbage cytoplast male sterile material RC7 with stable sterility and economic character the same as that of corresponding maintainer line; and the further crossing with the Chinese cabbage cytoplast male sterile material RC7 as male parent and Chinese cabbage self-bred line 04S245 as male parent to obtain the Chinese cabbage hybrid. The Chinese cabbage hybrid Jingqiu-70, or RC7*04S245, has the features of early ripening, compact plant, high disease resistance, etc.

The invention discloses Yunnan red pear-1 metallothionein gene PyMT1 and a prokaryotic expression vector thereof, and the prokaryotic expression vector is a prokaryotic expression vector which contains the Yunnan red pear-1 metallothionein gene PyMT1. According to the invention, PyMT1 gene is cloned from Yunnan red pear-1; the over expression of the gene in bacterial cells is controlled by T7 promoter and lactose operon with the induction of IPTG; PyMT1 protein is synthesized and secreted into cytoplasm; the expression level is detected by SDS-PAGE; test results show that the expression level of the PyMT1 protein reaches a maximum when the expression is inducted by 1mM IPTG at 37 DEG C for 4 hours and when the expression is inducted by 0.5 mM IPTG at 37 DEG C for 4 hours; the vector adopted by the invention can successfully express a lot of Yunnan red pear-1 metallothionein; the test results also demonstrates that the Yunnan red pear-1 metallothionein gene PyMT1 of Yunnan red pear has a typical function of heavy metal ion tolerance of the metallothionein gene, which lays a foundation for further research of PyMT1 functions and the applications of the metallothionein gene in microbial tolerance to heavy metal.

The present invention provides methods and compositions for enhancing channel activity to the mutant cystic fibrosis trans-membrane conductance regulator protein (CFTR). The compositions of the invention comprise polypeptides containing CFTR sub-domains that are designed to mimic the folding defect of the full length mutant CFTR proteins, resulting in competitive binding to cytoplasmic chaperones such as Hsc/Hsp7O and Hdj2. The methods of the invention comprise transduction, or recombinant expression, of CFTR polypeptides in a cell expressing mutant CFTR. The presence of the CFTR polypeptide results in a dominant effect whereby the CFTR polypeptide competes with the endogenously expressed mutant CFTR for binding to cytoplasmic chaperones such as Hsc/Hsp70 and Hdj2. Mutant CFTR proteins include, but are not limited to, DeltaF508 CFTR. The present invention is based on the discovery that reduced binding of cytoplasmic chaperones to the endogenous DeltaF508 CFTR, mediated by the presence of CFTR polypeptides, results in restoration of plasma membrane localization and channel activity. The methods and compositions of the invention can be used to restore channel activity in cystic fibrosis subjects carrying genetic defects in the CFTR gene, such as for example, DeltaF508 CFTR.

The invention discloses a celery cytoplasmic male sterile line breeding method and a cross-breeding method using a celery cytoplasmic male sterile line. In the methods, celery '021542' serving as a female parent and chervil '022047' serving as a male parent are subjected to manual control hybridization, male sterile plants are selected from an F1 group as a cytoplasmic male sterile source and is subjected to fifth backcross generation with the celery '021542' to obtain a celery cytoplasmic male sterile line '0863A'; and a backcross male parent corresponding to the celery cytoplasmic male sterile line is a maintainer line '0863B'. The sterile line '0863A' and a selected male parent line are subjected to field planting in a cross-breeding farm with an excellent isolation condition in a ratio of 3:1; and after pollination is finished, male parent plants are uprooted completed, and seeds picked from sterile line plants after the yellow maturity of the seeds are generation hybrids. The cytoplasmic male sterile line bred by the method has stable heredity, high combining ability, simple cross-breeding process, low cost and high quality of the hybrids, and can be used for the large-scale cross-breeding production of celery.

The invention discloses a method for identifying the purity of hybrid soybeans seeds by molecular markers, comprising the following steps of: using identified hybrid soybean varieties improved by RN cytoplasmic male sterility triple system and parental gene groups DNA thereof as a template, by SSR (simple sequence repeat) analysis, establishing SSR specificity fingerprint of the hybrid soybean and respective parents, successfully obtaining codominant an SSR marker which can distinguish the parents and the hybrid seeds definitely, and carrying out seed purity detection of the identified hybridsoybean variety improved by the RN cytoplasmic male sterility triple system according to the specificity markers. The result and the field identification are consistent by random group identificationof the hybrid soybean varieties. The method is simple and convenient to operate, the seed purity of the hybrid soybean varieties improved by the RN cytoplasmic male sterility triple system can be fast, accurately and reliably identified by experiment means through the screened specific molecular markers, so that the risk causing possible production reduction due to insufficient purity of the seeds in the sales process of the hybrid soybean seeds can be reduced.

The invention relates to a method for breeding a cytoplasmic male sterile line of quality disease-resistant photo periodical insensitive hibiscus cannabinus. The method relates to integrating high quality, disease resistance and photo periodical insensitive performance and other outstanding properties to hibiscus cannabinus; the cytoplasmic male sterile line of hibiscus cannabinus is used as a female parent, the quality, disease-resistant and photo periodical insensitive hibiscus cannabinus is used as a male parent; the female parent and the male parent are subjected to saturation hybridization to obtain a hibiscus cannabinus cytoplasmic male sterile line which has the growth characteristics of photo periodical insensitive performance, high quality, disease resistance, growth stability, high economy and high combining ability. The hibiscus cannabinus sterile line bred by the method can effectively utilize the advantages of photo periodical insensitive performance and hybrid, the hibiscus cannabinus planting area can be widely expanded to international and national tropical zones with low latitude for annual planting, and therefore, the international core competitiveness and original innovation level of domestic cytoplasmic can be greatly increased.

The invention relates to the field of genetic engineering and particularly to a restoring gene RfoB and an expression vector and application thereof. RfoB utilizes a Brassica oleracea L. Ogura CMS cytoplasmic male sterile line as an object, and according to the restoring gene Rfo sequence, through the codon preference of the Brassica oleracea L. gene, a novel RfoB sequence is artificially synthesized. A PRfo promoter, an RfoB gene and a terminator E9-T are fused to form an RfoB Gene expression kit. After construction of the plant expression vector, through the Agrobacterium tumefaciens-mediated direct transformation method, Brassica oleracea L. Ogura CMS cytoplasmic sterile line is directly transformed so that the transgenic plant of Brassica oleracea L. is obtained. The fertility is restored. The transgenic plants are self-crossed and normally seeded, and they are introduced into other Brassica oleracea L.-like Ogura CMS cytoplasmic sterile line as a male parent so that the individualcarrying the RfoB gene can restore fertility.

The invention relates to wheat fertility recovery gene Rf6 molecular tag and its obtaining method and belongs to crop breeding and production fields. F2 population arising from wheat T.um-bellulatum translocation line N2114 with susan is analysed in individual genotype and its fertility. Codominant SSR mark MAG218 and dominant mark gwm88 is obtained, which are closely linked to T cell cytoplasm sterility fertility recovery gene Rf6, and can determine the existence of Rf6 and forecast the wheat plant fertility, so can screening the Rf6 plant rapidly.