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Pepper sterility restoring gene closely linked molecular marker, method and application

A technology for restoring genes and molecular markers, applied in the field of molecular biology, can solve the problem of closely linked molecular markers of pepper cytoplasmic male sterility restorer genes that have not yet been discovered, and achieve the three-line hybrid seed production of peppers and molecular marker-assisted selection breeding of restoration materials Fast and convenient, speed up the breeding process, improve the effect of breeding efficiency

Active Publication Date: 2017-11-03
河南省农业科学院园艺研究所
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Problems solved by technology

Although so many molecular markers have been published, no molecular marker closely linked to the pepper cytoplasmic male sterility restorer gene has been found so far

Method used

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  • Pepper sterility restoring gene closely linked molecular marker, method and application
  • Pepper sterility restoring gene closely linked molecular marker, method and application

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[0027] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments, but should not be construed as a limitation of the present invention. The experimental methods in the following examples, unless otherwise specified, are conventional methods, and the materials, reagents, etc. used in the following examples, unless otherwise specified, can be obtained from commercial sources.

[0028] The present invention provides a molecular marker of a molecular marker closely linked with pepper sterility restoration gene, said molecular marker is scd06-17, including the nucleotide sequence shown in SEQ ID NO.1 and the nucleotide sequence shown in SEQ ID NO.2. The nucleotide sequence shown.

[0029] The molecular marker scd06-17 is amplified by the upstream primer (Scd06-17F) sequence shown in SEQ ID NO.3 and the downstream primer (Scd06-17R) sequence shown in SEQ ID NO.4, wherein the A specific fragment of 342bp can be amp...

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Abstract

The invention belongs to the technical field of molecular biology and discloses a pepper sterility restoring gene closely linked molecular marker, a method and application. The molecular marker is scd06-17 and comprises a nucleotide sequence shown as SEQ ID NO.1 and a nucleotide sequence shown as SEQ ID NO.2. The molecular marker is stable and reliable in result and is a co-dominant molecular marker, whether a pepper material contains a cytoplasmic male sterility restoring gene or not can be determined in a pepper steeling stage, and accordingly breeding efficiency is improved, and a breeding process is accelerated. Compared with other domestic and international published pepper restoring gene molecular markers, the pepper sterility restoring gene closely linked molecular marker is significant to pepper three-line hybrid seed production and restoring material molecular marker assisted selection breeding.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a molecular marker closely linked with pepper sterility restoration gene, a method and an application. Background technique [0002] The use of CMS fertility gene (Cytoplasmic male sterility) for three-line matching seed production of pepper can save manual castration, improve seed purity, and is conducive to the protection of intellectual property rights. However, if the fertility recovery of the male parent is not strong, it will seriously affect the fruit shape and yield of F1. Therefore, breeding restorer lines (male parents) with strong restorative ability, high combining ability and excellent agronomic traits has become one of the main problems urgently needed to be solved in the utilization of heterosis in pepper CMS. The traditional selection of CMS fertility genes in pepper is based on the fertility performance of testcross combinations. Molecular...

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张强魏小春姚秋菊常晓珂杨双娟王志勇赵艳艳张晓伟原玉香蒋武生郑直
Owner 河南省农业科学院园艺研究所
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