30results about How to "Reliable results" patented technology

This invention provides a gene related to loquat flowering. SVP1 This invention relates to the field of molecular biology, specifically to a tightly linked SNP marker and its applications. The nucleotide sequence of this SNP marker is shown in SEQ ID NO.1. A genotype of C at the locus corresponds to a spring-flowering phenotype in loquat, while a genotype of G corresponds to an autumn-flowering phenotype. The invention also provides specific primers (SEQ ID NO.2 and SEQ ID NO.3) for detecting this SNP marker, a kit containing these primers, and a screening method for detecting loquat flowering time using this marker. By extracting loquat DNA and performing PCR amplification and sequencing analysis, the flowering period of loquat can be quickly and accurately determined. The SNP marker of this invention is highly linked to loquat flowering time, providing an effective tool for marker-assisted selection breeding of loquat at different flowering periods. This significantly improves breeding efficiency, shortens the breeding cycle, and is of great significance for extending the loquat fruit supply period and optimizing the industrial structure.

The present application relates to the field of bioengineering technology, and more particularly to the application of the amt gene and mutant strain M107 in expressing siderophore of nitrogen-fixing bacteria. The present application uses Tn5 transposition technology to construct a mutant library, and selects mutant strain M107 with significantly improved siderophore synthesis capacity compared with wild strain GXGL-4A. The insertion site of the mutant strain M107 is the aminomethyltransferase gene amt. Through CAS plate detection and relative content determination of siderophore, it is confirmed that the target mutant strain M107 has significantly improved siderophore yield compared with the wild strain, and the bacterial growth is not affected; the growth promotion experiment on cucumber shows that the obtained high-yield siderophore mutant strain has a significant growth promotion effect on cucumber. Compared with the prior art, the present application significantly improves the siderophore yield of the nitrogen-fixing bacteria by mutating the amt gene of the nitrogen-fixing bacteria, and the obtained mutant strain can be used as a plant growth promoting bacteria, which maintains the nitrogen fixation activity while increasing the absorption of iron in the soil.

This invention discloses a rapid prediction method for rail rolling contact fatigue damage in the field of rail damage prediction technology. The method includes establishing a three-dimensional elastoplastic wheel-rail cyclic rolling contact finite element model, improving the cyclic plastic constitutive model, and simulating the stress-strain evolution of the rail. A rail stress-strain sample library is generated through parametric sampling and batch calculation. A Physically Constrained Generative Adversarial Network (PC-GAN) is constructed, embedding physical constraints such as contact force conservation and energy consistency into the loss function to achieve rapid and high-precision mapping from operating parameters to the stress-strain field. Based on the PC-AN prediction results, cumulative damage and fatigue life are calculated using an incremental multiaxial fatigue damage model, and a damage distribution map of the entire rail line is drawn, identifying high-risk sections. This invention deeply integrates physical modeling with intelligent algorithms, achieving an order-of-magnitude improvement in rail fatigue damage assessment efficiency while ensuring prediction accuracy, providing efficient and reliable technical support for rail life assessment and maintenance decisions across the entire rail line.

The application discloses a magnesium alloy inorganic coating adhesion test method and relates to the technical field of surface performance test. The method comprises the following steps: tape sticking: covering a tear tape on the surface of the inorganic coating of a magnesium alloy sample to be tested, and extending one end of the tear tape to form a tear section; sample positioning: fixing the magnesium alloy sample to be tested, so that the surface of the inorganic coating forms an angle of 90 degrees plus or minus 2 degrees with the direction of gravity of the magnesium alloy sample to be tested; dynamic peeling: applying a transient impact load to the tear tape through the tear section, so that the tear tape falls off and the inorganic coating is partially peeled off; and adhesion calculation: based on the percentage of the residual area of the inorganic coating, the adhesion value of the inorganic coating of the magnesium alloy sample to be tested is obtained through a pre-established corresponding relationship model between the percentage of the residual area of the inorganic coating and the adhesion value tested by the pull-out method. The application can realize instant quantification of the adhesion, the result is reliable, and the method can directly guide process adjustment and quality control on the production site.

A kind of ship shafting automatic centering correction system, first laser displacement sensor group being arranged between the output flange of ship main engine and intermediate shaft, second laser displacement sensor group being arranged between intermediate shaft and tail shaft, multiple degrees of freedom hydraulic adjusting actuator being respectively installed on main engine pedestal and tail shaft support, data acquisition module being signal connected with first laser displacement sensor group and second laser displacement sensor group, central processing unit being communication connected with data acquisition module, and drive control module being connected with central processing unit control signal output end.This application adopts two groups of laser ranging probe arranged along circumference, respectively arranged in the connecting area of main engine output flange to intermediate shaft and intermediate shaft to tail shaft, covers the interface of propelling shafting from space, through synchronous trigger unit and main shaft speed encoder hard line connection, eliminates false misjudgment caused by different sampling phase.

This invention provides a method for detecting AAV antibody titers, belonging to the field of biomedical technology, comprising: S1 preparing serum-free AAV vectors with different MOI values; preparing a mixture containing different volumes of test serum samples and AAV vectors with known MOI values ​​for co-incubation; S2: adding plasmids containing the CRISPR-Cas9 gene editing system to electroporation buffer, performing electroporation on a known number of cells, adding the serum-free AAV vectors and mixture from step S1 to the electroporated cells respectively, and detecting the expression efficiency of the reporter gene after electroporation; S3: calculating the AAV antibody titer in the test serum sample based on the detection results. The main advantage of this invention is that it can display AAV antibody titers digitally and quantitatively, facilitating understanding and communication, and obtaining stable and reliable results using different cell lines and different AAV transduction doses. This method is easy to standardize and validate, saving time, effort, and detection costs compared to traditional methods, and directly reflects antibody neutralizing activity.

This invention provides a residual-temperature assisted multi-heat-source synergistic modulation welding device and method, relating to the fields of laser welding and friction stir welding, aiming to solve the problems of easy forming defects and performance degradation in welding aluminum-lithium alloys. The device outputs a composite heat source through a laser-arc composite heat source system to melt the welding wire and stabilize the molten pool with deep penetration on the front side of the workpiece; a motion system controls the movement of the friction stir heating system; a residual-temperature-airflow synergistic auxiliary temperature control system dynamically adjusts the temperature of the solid phase region of the molten pool; a monitoring and sensing system collects physical field modal data in real time and uploads it to the control system; a flexible tooling system is used to clamp the workpiece with variable curvature; the friction stir heating system applies dynamic shearing and mechanical upsetting forces in the residual high-temperature region on the back side, effectively improving defects such as back-side collapse and burn-through in the weld of the workpiece with variable curvature. The residual-temperature assisted multi-heat-source synergistic modulation welding method is applied to the residual-temperature assisted multi-heat-source synergistic modulation welding device.

This application provides a method and system for predicting the fatigue performance of composite materials based on infrared thermal imaging, belonging to the field of material mechanical property testing technology. The method includes determining the fatigue performance characterization parameters and corresponding static strength values ​​of the composite material to be predicted; constructing a fatigue performance testing system for the composite material; determining the progressively increasing fatigue loading stress and load; applying progressively increasing stress levels of fatigue cyclic loading to the composite material under a given cycle to obtain a temperature field sequence and extracting temperature characteristic parameters characterizing the material's energy dissipation state; based on the temperature characteristic parameters and a composite material damage infrared characteristic library, obtaining the temperature-damage characteristic-stress relationship of the composite material under each cyclic loading level; based on this relationship, obtaining the critical transformation stress of the composite material to be predicted, determining the fatigue limit, and obtaining the fatigue life-stress relationship for fatigue performance prediction. This scheme improves prediction efficiency and accuracy, and avoids misjudgment based on a single parameter.

The application discloses a method for determining multi-target quaternary ammonium salt compounds in blood based on ultra-high performance liquid chromatography-tandem mass spectrometry, and relates to the technical field of drug and poison analysis and detection, and comprises the following steps: preparing a mixed standard working solution, wherein the mixed standard working solution contains paraquat, diquat, chlormequat, mepiquat, pipradrol, tubocurarine, didecyldimethylammonium chloride (DDAC), neostigmine, succinylcholine chloride, vecuronium bromide and succinylcholine chloride; and determining the multi-target quaternary ammonium salt compounds in blood based on ultra-high performance liquid chromatography-tandem mass spectrometry, wherein the contents of paraquat, diquat, chlormequat, mepiquat, pipradrol, tubocurarine, didecyldimethylammonium chloride (DDAC), neostigmine, succinylcholine chloride, vecuronium bromide and succinylcholine chloride are determined simultaneously. The method is simple in operation, sensitive in detection and reliable in result, and is suitable for poisoning diagnosis and clinical toxicology research.

The application relates to the technical field of early warning fastener, in particular to a tower crane anti-loosening early warning bolt, an early warning nut and an early warning fastening device. The tower crane anti-loosening early warning bolt comprises a bolt body, a first elastic contact assembly, a first PCB, a first battery, a sealing cover and a first alarm. The bolt body comprises a head and a shank which are connected with each other. The first PCB is fixed on the end face of the head away from the shank. The first battery and the first alarm are both mounted on the first PCB and are seamlessly wrapped by the sealing cover. The head is provided with penetrating holes, and one first elastic contact assembly is mounted in each penetrating hole. One end of the first elastic contact assembly is fixed to the first PCB, and the other end is exposed from the head when not compressed. The alarm mechanism is arranged on the bolt, which makes up for the deficiency of alarm failure caused by possible loosening of the early warning nut. The early warning bolt collects the pressure information of the first elastic contact assembly through the first PCB, and when the early warning bolt is loosened, the pressure is reduced, so that the first alarm can be started to give an alarm.

This invention relates to the field of materials testing technology, specifically to a method for testing powder X-ray diffraction (XRD) samples without impurity peaks based on directional pelleting, which is particularly suitable for XRD testing of small quantities of powder samples. This method, through pelleting combined with glass slide elevation, solves the problems of powder scattering and impurity peak interference when the sample quantity is insufficient in existing technologies, thus improving the reliability and flexibility of XRD testing.

The application relates to the technical field of nanobiosensors, and discloses a bimodal nanoprobe for uric acid detection and a preparation method and application thereof. The bimodal nanoprobe is a fluorescence-photothermal bimodal nanoprobe, and is a core-shell structure nanoparticle, which comprises, from the inner layer to the outer layer, a) a fluorescent inner core composed of carbon dots and a silica layer coated outside the carbon dots; b) a manganese dioxide layer coated outside the fluorescent inner core; and c) a molecularly imprinted polymer layer coated outside the manganese dioxide layer. The bimodal nanoprobe in the application integrates a fluorescence signal source (carbon dots), a signal modulation and photothermal unit and a specific recognition layer (a uric acid molecularly imprinted polymer) through ingenious structure design, realizes bimodal sensing of "specific recognition -> MnO2 etching -> fluorescence recovery / photothermal weakening" on UA, and effectively solves the problems of poor selectivity, complicated operation and high cost of traditional methods.

This application discloses an automated sewing device, including a conveying mechanism, a sewing machine, a workpiece moving assembly, and a robotic arm. In this application, the workpiece moving assembly, the sewing machine, the robotic arm, and the conveying mechanism are combined to form an automated system. This system can automate workpiece sewing without human intervention, solve the difficulties of manual sewing, and save labor and time costs. At the same time, the taking off of the first workpiece and the insertion of the second workpiece can be closely combined. The mechanical device replaces the manual zipper movement process, saving labor and ensuring reliable results.

The application belongs to the technical field of aircraft engine part machining, and relates to a method for machining a multilayer stepped honeycomb workpiece of an aircraft engine and an electrode grinding wheel. According to the final design size and the original blank size of the multilayer stepped honeycomb workpiece, the radial and axial grinding allowances of each layer of the honeycomb workpiece are calculated, and through a plurality of rounds of electric spark machining parameter tests, the radial and axial step sizes of the multistep forming electrode grinding wheel are determined, and a multistep forming electrode grinding wheel that is shaped according to the structure of the multilayer stepped honeycomb workpiece to be machined is manufactured. After the workpiece to be machined is clamped and positioned, synchronous electric spark grinding machining is performed to obtain the multilayer stepped honeycomb workpiece. The application adopts the method for machining the multilayer stepped honeycomb by using the multistep forming electrode grinding wheel and electric spark grinding machining process parameters to grind the multilayer stepped honeycomb at one time, reduces the number of rough and fine grinding of the honeycomb, saves labor, improves production efficiency, and shortens the machining cycle of the multilayer stepped honeycomb.

This invention belongs to the fields of plant genetic engineering and biotechnology, and discloses a peanut endogenous strong promoter P. AhEF1α Its preparation methods and applications. Promoters include P AhEF1α‑1 P AhEF1α‑2 P AhEF1α‑3 and P AhEF1α‑4 This invention uses the Arabidopsis EF1α gene amino acid sequence as a reference, identifies candidate peanut EF1α genes through homology search, screens high-expression genes by expression level analysis, and clones the aforementioned promoter from the peanut genome of Yuhua 9326 using PCR technology. A recombinant vector containing this promoter is constructed and transformed into peanut embryogenic callus tissue. Functional verification shows that the promoter can efficiently drive the expression of downstream genes in peanut callus. This invention solves the technical bottleneck of existing peanut gene editing technologies that rely on exogenous promoters and have poor adaptability, fills the gap in the application of strong endogenous promoters in peanuts, and can be used to construct a precise gene editing system for peanuts, promoting stress-resistant, high-quality, and grade-equal peanut varieties. It has significant practical value and industrialization prospects.

This invention relates to the interdisciplinary field of molecular biology and bioinformatics, specifically to a method and system for designing multi-site mutation primers based on rule constraints and multi-objective optimization. The method includes the following steps: receiving the target DNA sequence and mutation site information; automatically grouping the mutation sites according to their position distribution and spacing in the DNA sequence; determining the corresponding primer design region by extending a fixed length upstream and downstream from the center position of each mutation group; generating multiple candidate primer sequences based on the primer design region using a sliding window method; constructing a multi-objective optimization scoring function based on annealing temperature, GC content, and primer length to comprehensively score and screen the candidate primers to obtain the optimal primer; optimizing the 3' end of the optimal primer to preferentially contain stable bases; and outputting the optimized primer sequence and related design parameters and explanation information, effectively solving the pain points of existing multi-site mutation primer designs.

This application provides a half-cavity directly coupled magnetic alloy high-frequency cavity, relating to the field of ion accelerator technology. The magnetic alloy high-frequency cavity is connected to a radio frequency power source. The magnetic alloy high-frequency cavity includes: a cavity body comprising two symmetrically arranged half-cavities; a vacuum pipe disposed inside the cavity body and connecting the two half-cavities; multiple magnetic alloy rings disposed in a single half-cavity and coaxially arranged with the vacuum pipe, the multiple magnetic alloy rings being parallel to each other; a coupling device for electromagnetically coupling the multiple magnetic alloy rings located in a single half-cavity to form an overall load unit; and an impedance transformation unit disposed on one side of each half-cavity and electrically connected to the overall load unit, used to transform the impedance of the overall load unit to an impedance matching the characteristic impedance of an external feed line, so as to connect to the radio frequency power source through the external feed line. This application, through a half-cavity fully coupled design, eliminates inter-path coupling, ensuring that the port impedance test value is consistent with the actual value, and is easy to adjust.

The application discloses a method for accurately testing compaction degree of fine-grained soil of built dam, which comprises the following steps: step one, drilling and taking undisturbed samples; step two, testing natural density and natural water content of each undisturbed sample; step three, calculating dry density of each undisturbed sample; step four, classifying fine-grained soil and taking disturbed samples of each kind of soil; step five, carrying out compaction test on each disturbed sample; and step six, calculating and determining compaction degree of each undisturbed sample. The application effectively solves the problem that the prior art cannot accurately test compaction degree of fine-grained soil at different depths of built dam, and has the advantages of being capable of accurately testing compaction degree of fine-grained soil at different depths of built dam, and the testing result is accurate and reliable.

The present application relates to a kind of monocotyledon green wisteria rapid propagation method, using the characteristics of green wisteria specific tissue cell totipotency, under open condition, using liquid medium to induce green wisteria specific site of in vitro leaf split differentiation, produce regenerative root and regenerative bud, finally form plant.The present application aims at the rapid propagation method of green wisteria leaf, without sterile environment, convenient to take material, easy to operate, low in cost, short in period, high in survival rate, with the advantages of high efficiency, economy, easy to scale, factory, etc., in green wisteria rapid seedling, specific material preservation and propagation has greater application value.

This invention proposes a droplet digital PCR method for simultaneously detecting the quantity of four foodborne pathogens. The steps include: 1. Preparing the DNA sample to be tested; 2. Preparing primers and probes for the four bacteria to be tested; the four bacteria to be tested are Staphylococcus aureus, Listeria monocytogenes, Salmonella typhi, and Bacillus cereus; 3. Using a ddPCR amplification reaction system, which includes the primers and probes for the four bacteria to be tested in step 2, amplifying the DNA sample to be tested in step 1, detecting the amplification products, and obtaining the quantitative results of the four bacteria. This invention can simultaneously detect four pathogenic microorganisms: Staphylococcus aureus, Listeria monocytogenes, Salmonella typhi, and Bacillus cereus. It establishes a precise, rapid, and absolute quantitative detection system for the simultaneous detection of these four pathogens using multiplex ddPCR technology, exhibiting high specificity, high precision, and strong repeatability.

An analytical method for determining the trapped oil pressure and its extreme values ​​in gear pumps / motors includes the following steps: Step 1, determining the trapped oil region and its positional variables based on the gear pair meshing process; Step 2, an analytical solution method for the trapped oil pressure; Step 3, a fitting formula method for obtaining the unloading area; Step 4, an analytical solution method for determining the extreme trapped oil pressure and its occurrence location. This invention offers a simpler method, more reliable results, and more accurate extreme values ​​and their occurrence locations; it significantly simplifies existing numerical methods for determining trapped oil pressure; it clarifies that the extreme value of the trapped oil pressure occurs at the point where the unloading groove is about to close, rather than at the actual closure point, and the specific extreme value location depends on the size of the unloading area in the area where the unloading groove is about to close; the method, obtained from 3D feature measurement of the unloading area and an Excel trend line, is highly efficient, fast, and accurate, providing a reliable basis for the accurate evaluation of the extreme trapped oil pressure in external gear pumps / motors and effective control in product design.

The application discloses a method for determining the minimum buried depth of a compressed energy storage underground gas storage, comprising the following steps: setting basic parameters of the compressed energy storage underground gas storage according to geological data of rock and volume requirements of the gas storage; considering factors of gas storage pressure, rock gravity, shear strength of surrounding rock and broken surface of the surrounding rock under pressure, establishing a vertical mechanical equation of the gas storage by adopting a conical body model, and obtaining a vertical buried depth capable of meeting maximum gas storage pressure requirements; and establishing a horizontal mechanical equation of the gas storage according to the basic parameters and the vertical buried depth of the gas storage, and obtaining a horizontal buried depth capable of meeting the maximum gas storage pressure requirements. Whether the vertical buried depth of the gas storage meets the limit inflation pressure is determined first, and then the horizontal broken surface mechanical equation is substituted into the horizontal buried depth on the basis of the vertical buried depth, so as to determine whether the horizontal buried depth meets the limit inflation pressure. The calculation method is simple, fast, accurate, and has good economic applicability.

The present application relates to a kind of well sinking opening mud burst determination method. Whether the well sinking opening of pipe jacking construction will appear mud burst, mostly based on engineering experience determination, result is unreliable. The present application obtains the design parameter of well sinking opening, the specific gravity of soil body around well sinking, and the cohesion and internal friction angle of soil body;The gravity of unit width inside mud burst body, groundwater seepage force of unit width inside mud burst body, the pressure of top of unit width inside mud burst body, and the damage force when unit width inside mud burst appears are calculated;The resistance of top of unit width inside mud burst body, the resistance of bottom of unit width inside mud burst body, and the total resistance of unit width inside mud burst body are calculated;The damage force when unit width inside mud burst appears and the total resistance of unit width inside mud burst body are compared, and the determination result is obtained. The present application judges whether mud burst will appear by combining the damage force of mud burst and the resistance of mud burst, the principle is clear, the operability is strong, and more reliable result can be obtained.

The present application relates to the technical field of solar cells, and particularly relates to a monitoring method for the passivation quality of a BC cell phosphorus diffusion considering RCA cleaning state; comprising the following steps: double-side alkali polishing is performed on a first type silicon substrate; after double-side deposition of amorphous silicon layer, second type element doping is performed, and acid washing is performed to remove glass body; then RCA cleaning is performed; after double-side deposition of amorphous silicon layer, first type element doping is performed to obtain a monitoring piece; the monitoring piece is detected, and the production line state is adjusted according to the monitoring result as needed; the monitoring method can consider the interference of RCA cleaning state in the actual production line, and is more in line with the actual process of phosphorus diffusion passivation monitoring process; the method result is accurate and reliable, and RCA interference caused by the mismatch of the monitoring piece structure is fundamentally eliminated, so that the final passivation quality test result can truly and accurately reflect the passivation level of the production line phosphorus diffusion process, and provide a reliable basis for accurate adjustment of process parameters.

This invention provides an auxiliary kit for detecting intracellular calcium ion content, consisting of a concentrated culture medium additive and an auxiliary solvent. It improves the sensitivity and precision of the calcium ion fluorescent probe method for detecting intracellular calcium ion influx by maintaining the physiological function of the test cells, preventing premature hydrolysis of the fluorescent probe precursor, improving the solubility and stability of the fluorescent probe precursor, and inhibiting the leakage of activated fluorescent probes. Changes in intracellular calcium ions participate in various cellular physiological activities and are commonly detected in drug development, basic biological research, and other fields. The auxiliary kit provided by this invention can help researchers measure intracellular calcium ion influx more conveniently and accurately, obtaining more reliable results.

The application relates to a detection mechanism, method, device and system of a fuel evaporation system, wherein the detection mechanism of the fuel evaporation system comprises a carbon tank, a valve part and a vane pump which are communicated with the carbon tank respectively; the valve part is provided with an intake manifold communication port and a fuel tank communication port; the vane pump comprises an impeller and a rotating speed sensor which is used for detecting the rotating speed of the impeller. The detection mechanism of the fuel evaporation system can improve the leakage detection efficiency of the fuel evaporation system, simplify the overall structure and improve the detection accuracy.

The present application relates to a kind of based on electrodeless resistivity Reinforced concrete corrosion state detection method, comprising the following steps: the prepared reinforced concrete sample vacuum drying treatment is placed in test device, with electrolyte solution composition series circuit, electrodeless resistivity meter is tested to obtain system total resistance, again test obtains electrolyte solution resistance, the resistance of steel in concrete is obtained by calculation.By the change of steel in concrete in different curing age, the corrosion rate of steel in concrete is calculated, compared with relevant standard, the corrosion state of steel in concrete is obtained.The present application adopts electrodeless non-contact method, eliminates the influence of contact resistance and contact capacitance between electrode and concrete on measurement, will not produce electrode polarization and other problems, it is convenient and fast, the result is expressed in the form of steel corrosion depth rate, the result is more intuitive, consistent with the result of weight loss rate of steel corrosion in the current national standard, the same state of steel is distinguished.

The application discloses an InDel molecular marker for identifying MeiGua single and double petal characters, a primer and application thereof, and comprises two InDel molecular markers, namely InDel 9 located at 5549358~5549382bp of a No.1 chromosome of MeiGua and InDel 90 located at 6309277~6309290bp of the No.1 chromosome of MeiGua. The application also develops a primer for amplifying the two InDel markers. The molecular marker can be used for quickly and efficiently identifying MeiGua single and double petal varieties at a seedling stage of MeiGua, and the accuracy rate reaches 100%. Compared with traditional breeding which needs to wait for about four years for flowering, the marker has the advantages of early time, low cost, accuracy and rapidness when applied to assisted breeding.