The invention discloses a stress sensor calibration system, which comprises a stress tester, a stress sensor and a secondary instrument, wherein the stress tester comprises a base, a lever bracket, a lever, a weight loading mechanism, an upper clamp and a lower clamp. The stress tester of the stress sensor calibration system applies a force by using a weight and provides a standard reference value of the stress by weight magnitude conversion; loading, unloading and loading protection of the stress tester are realized by adjusting the weight, and the stress of a tested piece is constant once the weight is determined, so the force value measurement precision is high; meanwhile, the lever pivot of the stress tester is a bearing and has strong bearing capacity, the upper clamp is hinged with the lever, and the lower clamp is hinged with the base, so that the pull and the pressure of the tested piece are on the same straight line, and the accuracy of the measured force value can be furtherensured; therefore, the stress sensor calibration system can accurately calibrate the output value of a sensor, and has high calibration precision.