The invention discloses a particle printing method,
system and device based on fluorescent field and bright field
time sequence imaging, and the method comprises the steps: monitoring a micro-fluidic
chip sorting region under the condition of continuous illumination of a multi-color fluorescent field, and collecting a fluorescent image of the micro-fluidic
chip sorting region in real time; when the target fluorescent particles are recognized in the fluorescent image, bright field illumination is automatically triggered, and a
bright field image is collected; carrying out fusion analysis
processing on the
fluorescence image and the
bright field image, and extracting multi-dimensional feature parameters; judging whether the multi-dimensional characteristic parameters meet printing conditions or not according to a preset multi-
modal judgment rule; when the multi-dimensional characteristic parameters meet printing conditions, a printing instruction is sent; and in response to the printing instruction, controlling the pressure and
motion control unit to drive the printing channel of the micro-fluidic
chip to print the target fluorescent particles to the specified hole site of the
microwell plate. According to the particle printing technology combining the advantages of
fluorescence imaging and bright field imaging, comprehensive feature analysis and high-precision sorting of particles are achieved.