146 results about "Photobleaching" patented technology

The invention provides a photosensitive resin composition containing an anthracene methyl quaternary ammonium salt photosensitizer and application of the photosensitive resin composition, a structural main body anthracene ring of the photosensitizer is introduced into methylene substituted by a quaternary ammonium group at a position 10 and / or a position 9, and an acid radical or halogen is adopted as an equilibrium anion; compared with a commercially available anthracene photosensitizer, the photosensitizer has excellent fat solubility and good water solubility through introduction of the quaternary ammonium salt on the premise that the photobleaching property is retained, great potential is brought to formula design of the photosensitive composition, and development waste in a later development process can be greatly reduced; the method can be widely applied to the photo-curing fields of dry films, paints, coatings, printing ink, forming materials and the like.

The invention belongs to the field of fluorescence detection, and particularly relates to a ratio-type fluorescent nanoprobe for specifically detecting tetracycline antibiotics as well as a preparation method and an application method of the ratio-type fluorescent nanoprobe. The nano-probe is a europium modified zinc gallium germanate nano-material, and is prepared by amino modification, citric acid modification and europium modification of the zinc gallium germanate nano-material. The probe can be specifically combined with tetracycline antibiotics; the fluorescent probe has two different fluorescence emission intensities, so that interference can be effectively eliminated, and the detection accuracy is high. And the synthesis method is simple and low in cost. The ratio type fluorescent nanoprobe provided by the invention also can realize the change of different colors under an ultraviolet lamp, can realize quantitative visual detection of tetracycline by combining the RGB value and the tetracycline concentration change, and can realize rapid on-site detection of tetracycline in a sample without the help of a fluorescent instrument. The tetracycline fluorescent probe is stable in optical performance, not prone to photobleaching, high in tetracycline recognition capacity, wide in response range (0.2-70 [mu] M) and low in detection limit, and has remarkable industrial application potential.

The invention provides a rare earth complex as well as a preparation method and application thereof. The chemical general formula of the rare earth complex is (Ln < 1x > Ln < 2y > Ln < 31-x-y >) (A) m (B) n, the rare earth complex provided by the invention has a self-assembly behavior, spherical nanoparticles can be formed through molecular aggregation, under ultraviolet irradiation, internal ligand molecules are better protected, the photobleaching process of the rare earth complex under natural conditions is slowed down, and the rare earth complex has better stability and higher weather resistance in the natural environment.

The invention belongs to the technical field of industrial silane preparation, and provides a natural silane rotor and a preparation method and application thereof.The preparation method comprises the steps that a pyridoxal phosphate solution and a 4-amino ethyl cinnamate solution are mixed and then react to obtain pyridoxal phosphate cinnamyl ester; and mixing the pyridoxal phosphate cinnamyl ester solution and the amino silane solution, and reacting to obtain the natural silane rotor. The silane rotor has a good conjugated structure, is stable in chemical structure, can be stored for a long time, is good in light stability, and is not liable to cause a photobleaching phenomenon. Besides, conjugated single and double bonds capable of freely rotating exist in the molecular structure, a typical flexible intramolecular rotation characteristic is presented, the conjugated structure can freely rotate in a dilute solution, a surface sightseeing signal is weak, the free rotation phenomenon is limited in a thick solution, the surface sightseeing signal is strong, and the surface sightseeing signal is strong. Therefore, the device can be used as a molecular tool for monitoring the change of the thin consistency of the silane, and can provide real-time, lossless and visual detection support for the development of a silane modulation process.

The invention provides fluorescent carbon dots and fluorescent hydrogel based on solvent stimulation response discoloration and a preparation method and application of the fluorescent carbon dots and the fluorescent hydrogel, and belongs to the technical field of fluorescent materials and information encryption. The preparation method of the fluorescent carbon dots comprises the following steps: mixing a biomass carbon source and a solvent, and performing solvothermal reaction to obtain the fluorescent carbon dots, the biomass carbon source comprises one or more of buxus buxus, oriental cherry leaves, fructus forsythiae leaves and oak leaves; the solvent is ethanol. The biomass carbon dots are low in toxicity, excellent in optical stability and photobleaching resistance and high in ionic strength; the fluorescent carbon dots have double emission peaks of red light and blue light / green light, are different from single fluorescent carbon dots, and can provide more fluorescent information storage density for a fluorescent gel-based information orientation encryption system. The fluorescent color of the fluorescent hydrogel can be stably switched in an ethanol / water solvent, the gel structure is stable, the information storage capacity is improved, and a fluorescent hydrogel-based information anti-counterfeiting encryption system is promoted to develop towards multiple dimensions.

Provided herein are fluorescently-labeled nucleotide conjugates for nucleic acid analysis. Also provided are reagents used for forming binding complexes between a fluorescently-labeled nucleotide conjugate and a target nucleic acid sequence in the presence of one or more reagents disclosed herein. Binding complexes can be detected in the presence of the one or more reagents. For example, the one or more reagents may contain a photobleaching reducing agent configured to reduce photobleaching resulting from use of the fluorescently-labeled nucleotide conjugate to form the binding complex in a nucleic acid analysis. Such nucleic acid analysis may be used to identify sites of nucleobase binding or incorporation between the target nucleic acid sequence and one or more nucleotide moieties of the fluorescently-labeled nucleotide conjugate in a nucleic acid sequence reaction.

The invention relates to the technical field of nano materials and biomedical engineering, and discloses a dye-sensitized rare earth up-conversion luminescent nano material as well as a preparation method and application thereof. The dye-sensitized rare earth up-conversion luminescent nano-material comprises a rare earth doped up-conversion nano-particle core, an unsaturated fatty acid salt self-assembled shell layer and a near-infrared dye modified on the surface of the rare earth doped up-conversion nano-particle. The unsaturated fatty acid salt coating layer endows the material with excellent water dispersibility through a hydrophilic group; a hydrophobic chain segment and an unsaturated double bond can effectively block water and oxygen permeation and quench singlet oxygen, so that the photobleaching half-life period of the material is increased by 86.7 times or more compared with that of a conventional coating material, and the light stability is excellent; the material successfully realizes efficient up-conversion luminescence under excitation of a low-power near-infrared light emitting diode (NIR LED, the power density of which can be as low as 100mW / cm < 2 >), and thoroughly gets rid of dependence on a high-cost and high-risk laser.

This invention relates to aggregation-induced emission probes with specific responses to tumor markers and their applications, belonging to the field of biochemistry. This invention utilizes molecular structure modulation to prepare a series of molecules possessing large Stokes shifts, resistance to photobleaching, near-infrared II (NIR-II) emission, and aggregation-induced emission properties. Different self-eliminating groups are attached to the dimethylamino group of the molecule via quaternization or to the pyridine group via pyridinium salting. By controlling the structure of these self-eliminating groups, activatable luminescent probes responding to different tumor markers can be constructed. These probes integrate deep NIR-II imaging, aggregation-point luminescence, large Stokes shifts, and marker-specific responses, providing a new approach for precise imaging diagnosis of tumors.

The application discloses an aggregation-induced emission compound with double fluorescence emission characteristics and a preparation method and application thereof, and the proportional fluorescence probe has a structure of general formula I. The probe molecule has a polar response group tetraphenyl ethylene as a fluorescence report unit, introduces a hydrophilic oxygen atom, connects an alpha carbon of an acetamide, and connects a polar detection sensitization group Tag chain on an amino group of the acetamide. The probe molecule improves the conjugation degree of oxygen atom lone pair electrons and a tetraphenyl ethylene pi electron system, so that the probe molecule simultaneously produces n→pi* and pi→pi* molecular transitions, and a proportional fluorescence probe which can sensitively respond to polarity change is constructed. The fluorescence probe designed in the application has the advantages that the fluorescence probe is not easily interfered by probe concentration, a detection environment and light bleaching, and experiments prove that the proportional fluorescence intensity of the fluorescence probe can sensitively respond to polarity, and the proportional fluorescence intensity and water content are linearly fitted, so that the purposes of detecting water content in an organic reagent and alcohol content in wine are achieved.

The application discloses an organic compound biological probe and a preparation method and application thereof, and belongs to the field of fluorescent probes. The structural formula of the organic compound biological probe is as follows, and the preparation method comprises the following steps: adding 2,4-dihydroxybenzaldehyde and anhydrous potassium carbonate into anhydrous acetonitrile or DMF, then dropwise adding 1,4-dibromobutane to obtain a colorless oily compound 1 through reflux reaction; dissolving the compound 1 and triphenylphosphine in anhydrous acetonitrile to obtain a solid compound 2 through reflux reaction; dissolving 1,1,2-trimethyl-1H-benzo[e]indole and iodoethane in anhydrous acetonitrile to obtain a compound 3 through reflux; dissolving the compound 3 and the compound 2 in anhydrous ethanol, adding piperidine, and then performing reflux reaction and purification to obtain the organic compound biological probe. The organic compound biological probe can self-assemble into nanoparticles with a size of about 1.6 nm in water, has strong anti-photobleaching ability, and integrates red light and near-infrared dual-channel imaging, pH detection, mitochondrion targeting and chemotherapy.

The application discloses an aggregation-induced emission microsphere with colorimetric-fluorescent double signals and a preparation method and application thereof. The aggregation-induced emission microsphere comprises carboxyl polystyrene microspheres and aggregation-induced emission dyes embedded in the carboxyl polystyrene microspheres; and the structural formula of the aggregation-induced emission dyes is as follows: The aggregation-induced emission microsphere has colorimetric-fluorescent double signals, is red under natural light, has strong absorption at 600 nm, and shows high light-emitting efficiency at 725 nm, effectively avoids the interference of biological autofluorescence, reduces background interference, has good light stability and light bleaching resistance, and has high molar extinction coefficient, so that the aggregation-induced emission microsphere has colorimetric and fluorescent double signal output capabilities. In addition, the preparation process of the aggregation-induced emission microsphere has high reproducibility, has the potential for large-scale synthesis, and meets the needs of industrial batch production.

The invention discloses a photobleached all-polymer optical waveguide grating sensor and a preparation method thereof, and belongs to the technical field of polymer optical waveguide grating sensors. The preparation method comprises the following steps: carrying out hot stamping on a substrate sheet to obtain a substrate sheet with a groove; spin-coating photoresist on the substrate sheet to form a photoresist core layer; photoetching the photoresist core layer through double-beam interference to obtain the photoresist core layer with interference fringes; spin-coating a thermosensitive polymer cladding material on the photoresist core layer, covering the flexible substrate and then carrying out low-temperature thermocuring so as to bond the photoresist core layer and the flexible substrate; stripping the substrate sheet, exposing the photoresist core layer, covering the protective cladding, and curing to obtain a multi-layer composite device; and performing non-uniform post-baking on the multi-layer composite device to obtain the multi-layer composite device. According to the invention, the grating period can be changed under the condition that the grating mask is not replaced, and the cost of spectrum adjustment is greatly reduced.

The invention provides a method for detecting fusion of extracellular vesicles by adopting fluorescence resonance energy transfer, and belongs to the technical field of extracellular vesicles. A dynamic fluorescence monitoring system is established, low-power laser excitation is adopted, dual-wavelength fluorescence signals are monitored at the same time, fluorescence signal data are collected, graded gain regulation and control processing is carried out through five threshold values, and a fluorescence signal super-sparse matrix is constructed; establishing a fluorescence signal processing matrix model, converting the super-sparse matrix into a dense matrix, constructing a gain matrix for signal correction, calculating a fluorescence resonance energy transfer efficiency parameter, obtaining a standardized efficiency value through matrix operation, outputting an extracellular vesicle fusion kinetic analysis result, and establishing a quantitative evaluation system. The technical problems of poor signal stability and low detection precision in the process of fluorescence resonance energy transfer detection of extracellular vesicle fusion are solved.

This invention discloses an illumination intensity modulation method for disk scanning confocal imaging, comprising the following steps: S1: Before formal imaging, a pre-imaging is performed using weak, uniform illumination; S2: The fluorescence intensity of different regions in the pre-imaging is evaluated and analyzed, and the evaluation results are obtained; S3: Based on the evaluation results, the parameter settings of the intensity modulation mechanism are adjusted to achieve different illumination in different regions of the sample; S4: The scanning points traverse the imaging area to obtain a complete image on the camera. This invention can dynamically adjust the illumination intensity of local regions in the scanning imaging as needed, and can be used to reduce photobleaching in local regions, increase the dynamic range of imaging, and enhance the signal-to-noise ratio of local regions.

The invention relates to the field of heavy metal detection, in particular to a boron-doped carbon quantum dot and a preparation method and application thereof. In order to realize synchronous detection of dichromate radicals and permanganate radicals, curcumin and m-phenylenediamine are used as a carbon source and a nitrogen source, boric acid is used as a boron element doping agent, and the boron-doped carbon quantum dots are obtained by adopting a one-step solvothermal method. Wherein curcumin and m-phenylenediamine are used as precursors, boric acid is used as a boron element doping agent, and the boron-doped carbon quantum dots are synthesized. The preparation method is simple, and the prepared B-N-CDs has blue-green fluorescence, shows excellent biocompatibility and photobleaching resistance, is successfully applied to detection of Cr2O7 < 2-> and MnO4 <-> in water, is successfully applied to the field of fluorescent ink, and has potential value in the aspect of anti-counterfeiting marks.

The application discloses a pyridine cation mitochondrial fluorescent probe, the chemical name of the probe is 4-(8-dimethylamino-5-benzocoumarin)-methylpyridine iodine salt, and the chemical structural formula is shown as formula (I). The application further discloses application of the fluorescent probe in labeling or imaging display of mitochondrial distribution or morphology in living cells and in long-range tracking display of mitochondria in living cells and imaging display of nucleoli in cells. Experiments prove that the labeled mitochondrial fluorescent probe has high light bleaching resistance and a high fluorescence quantum yield, can realize long-range tracking of mitochondria in living cells, and can label fixed nucleoli in cells. It is indicated that the fluorescent probe has potential application and development value in the fields of fluorescent probes and tissue imaging, and has a wide application prospect.

The application provides a photosensitive resin composition containing an anthracene methyl quaternary ammonium salt photosensitizer and application thereof, the photosensitizer has a quaternary ammonium group substituted methylene introduced at 10 and / or 9 positions of the anthracene ring, and an acid radical or halogen is used as a counter anion; compared with a commercially available anthracene photosensitizer, the photosensitizer has excellent fat solubility and good water solubility by introducing the quaternary ammonium salt on the premise of retaining photobleaching, brings great potential for formula design of the photosensitive composition, and can greatly reduce developing waste in a later developing process; and can be widely applied in the fields of dry films, paints, coatings, inks and molding materials and the like.

The invention discloses a nitrogen-doped carbon dot and a rapid fluorescence detection method thereof for indoxacarb residue, and belongs to the field of biosensor technology and food quality detection. L-theanine and ethidene diamine are used as raw materials, the nitrogen-doped carbon dots are prepared through a hydrothermal method, the N-CDs have the advantages of being stable in fluorescence emission, good in salt tolerance and excellent in photobleaching resistance, and indoxacarb can specifically quench fluorescence through the static quenching effect. By optimizing N-CDs synthesis conditions and indoxacarb detection conditions, an indoxacarb fluorescence sensing detection system with high selectivity and high sensitivity is constructed. The detection method disclosed by the invention can be suitable for detecting indoxacarb residues in environmental water samples such as tap water and river water and agricultural product matrixes such as apples and tea leaves, the standard recovery rate is good, the relative standard deviation is low, on-site rapid screening of the indoxacarb residues can be realized, and a new technical means is provided for monitoring the quality safety of the environment and agricultural products.

The anti-photobleaching excitation light-induced fluorescence heavy metal detection system comprises a system control unit, a fluorescence excitation unit, a fluorescence detection unit and a data acquisition card; the system control unit sends a pulse excitation signal to make the fluorescence excitation unit generate pulse fluorescence; the fluorescence detection unit collects the pulse fluorescence and converts it into an electric signal input into the data acquisition card; the system control unit sends a fluorescence detection signal to make the data acquisition card save n fluorescence intensity values; and the data acquisition card averages the n fluorescence intensity values. The application avoids the problem of photobleaching of the fluorescence probe caused by long-time irradiation of the excitation light by shortening the irradiation time of the excitation light, reduces the error of the detection result and improves the service life of the fluorescence probe.

Provided is a method of screening for an autophagy modulator. According to the screening method of an aspect, the autophagy modulator can be accurately screened with only a single cell. In addition, screening can be performed without using fluorescent labeling, and thus cytotoxicity and photobleaching that occur when fluorescent labeling analysis methods are used can be prevented, and the limitation of having to monitor only fluorescently labeled markers can be improved.

A fluorescence target includes a first fluorescing region and a second fluorescing region. The first fluorescing includes a first population of fluorophores that emit first fluorescence when the fluorescence target is illuminated with fluorescence excitation illumination. The first fluorescing region photobleaches at a first photobleaching rate. The second fluorescing region is proximate to the first fluorescing region and includes a second population of fluorophores that emit second fluorescence when the fluorescence target is illuminated with the fluorescence excitation illumination. The second fluorescing region photobleaches at a second photobleaching rate that is different than the first photobleaching rate.

The invention provides anthracene methyl thiosulfonate for a photosensitive resin composition, and relates to the field of photopolymerization. The invention also comprises a photosensitive resin composition containing the anthracene methyl thiosulfonate, a photosensitive dry film containing the anthracene methyl thiosulfonate, and applications of the photosensitive resin composition and the photosensitive dry film. The anthracene methyl thiosulfonate provided by the invention has photosensitivity, can be used as a photosensitizer in a photosensitive resin composition, can improve the adhesive force of the photosensitive resin composition on various metal surfaces, has the characteristic of low migration, has good compatibility with monomers and solvents, and can be widely applied to the photocuring fields of dry films, paints, coatings, inks, molding materials and the like; and the photobleaching agent has extremely high photobleaching efficiency and is particularly suitable for being used in a formula of an ultra-thick film.

The invention discloses a micro / nano plastic based on organic long-afterglow material labeling and a preparation method and application thereof, and belongs to the technical field of crossing of environmental science and macromolecular nano materials. According to the method, organic long-afterglow material dispersion liquid is dropwise added into micro / nano plastic dispersion liquid, and swelling is balanced; and carrying out rotary evaporation to remove the swelling agent, washing, and dialyzing until the dialysate has no obvious ultraviolet absorption, so as to obtain the micro / nano plastic marked by the organic long afterglow material. According to the method, the characteristic that the organic long-afterglow material can realize high-brightness luminescence without in-situ excitation is utilized, and the background interference of an environmental medium is effectively inhibited, so that the signal-to-noise ratio and the sensitivity of micro / nano plastic detection are remarkably improved under the environmental concentration condition. Compared with an existing marking technology, the method has the advantages that the operation is simple, the cost is low, the biocompatibility is good, and the problems of false positive interference and photobleaching can be effectively avoided; good application prospects are shown in the fields of environment migration behavior research of micro / nano plastic, bioluminescence imaging and the like.

The invention provides a construction method of a light-stable high-photon-number fluorescent probe and application of the light-stable high-photon-number fluorescent probe in single-molecule and super-resolution fluorescence imaging. The probe is efficiently prepared through one-pot multi-component reaction of aldehyde containing cyclooctatetraene, amine containing benzothiazole methyl sulfone, fluorophore carboxylic acid with a click tag and tert-butyl isocyanide. The probe can realize cysteine site specific labeling through nucleophilic aromatic substitution, the cyclooctadiene light stabilizer can effectively quench triplet, photon output and photobleaching resistance are improved synergistically, and the probe and the cyclooctadiene light stabilizer are suitable for super-resolution fluorescence imaging of biomacromolecules such as single molecules and nucleopore proteins. And a high-performance probe is provided for an advanced super-resolution fluorescence microscopy technology.

Disclosed herein are compositions, systems, kits, and methods for photobleaching, and selective photobleaching, a biological sample.

The invention provides an SERS technology-based encrypted information read-write method and system, and the method comprises the steps: carrying out the irradiation of a thin film substrate through selected writing laser and a preset laser irradiation track based on the thin film substrate, so as to achieve the writing of encrypted information; the laser power of the write-in laser according to the demand is changed based on different write-in positions; and based on the selected reading laser, performing two-dimensional Raman imaging on a write-in area according to a laser irradiation track in an encrypted information write-in process by adopting a surface enhanced Raman scattering imaging technology, extracting Raman signal intensities at different positions, and restoring a drawn spectral pattern so as to realize the reading of the encrypted information. The method can be suitable for any laser wavelength capable of initiating the molecular photobleaching reaction, so that the selection of the laser wavelength is more flexible, the experimental design is simplified, and the experimental cost is reduced.

The present application relates to a kind of metal nanoparticle-based plasmonic enhanced fluorescence multilayer film optical structure and its preparation method and application, the multilayer film optical structure is by with self-assembly metal nanoparticle as enhancement substrate, to be measured fluorescent probe molecule is placed on the surface of the enhancement substrate and obtains.The present application is by the surface plasmon resonance of self-assembly metal nanoparticle, while effectively inhibiting fluorescent probe molecule photobleaching, greatly improves the sensitivity of fluorescence trace gas sensing, the service life of organic probe fluorescence and gas sensor reliability;Compared with the prior art glass substrate fluorescent film technology, the performance of the present application is comprehensively improved, and has good market application prospect.

A photosensitive resin composition capable of obtaining excellent whiteness, reflectivity, resolution, and high sensitivity, a dry film, a cured product, and an electronic component. The photosensitive resin composition is a photosensitive resin composition containing a carboxyl-containing resin (A), a phosphorus-based photopolymerization initiator (B), a photobleaching oxime ester-based photopolymerization initiator (C), and titanium oxide (D), wherein the photobleaching oxime ester-based photopolymerization initiator (C) has an absorbance of 0.01-0.20 in THF, at a concentration of 200 ppm, and at an optical wavelength of 405 nm, and has an absorbance of 0.10-1.00 at an optical wavelength of 385 nm, and with respect to 100 parts by mass of a solid component, namely, the carboxyl-containing resin (A), the blending amount of the photobleaching oxime ester-based photopolymerization initiator (C) is 0.2-1.5 parts by mass.

The present invention belongs to the technical field of biomedical detection and in-vitro diagnosis. Disclosed are a colorimetric-fluorescent dual-mode nanoprobe and microfluidic chip for detecting cMyBP-C. In the present invention, a colorimetric-fluorescent dual-mode nanoprobe, which comprises UCNPs as a core and MOF as a shell and is loaded with horseradish peroxidase and a labeled antibody, is prepared by means of a "layer-by-layer self-assembly" technique; and then a microfluidic chip is prepared on the basis of the nanoprobe. The microfluidic chip realizes dual-mode detection of colorimetric and fluorescent signals of cMyBP-C, thereby solving technical problems such as limited sensitivity, susceptibility to background interference, limited linear range, photobleaching and signal instability in single-signal detection.