254 results about "Photobleaching" patented technology

The invention provides a photosensitive resin composition containing a photobleaching aminomethyl anthracene photosensitizer and application thereof.The structural main body anthracene ring of the photosensitizer introduces an aminomethyl functional group to the 9th and / or 10th site: through introduction of the aminomethyl functional group, while the electron cloud density of polycyclic aromatic hydrocarbon is maintained, the maximum absorption wavelength is subjected to red shift, and the photosensitive resin composition is especially suitable for a 405nm illumination system; the initiation efficiency is obviously improved; the exposure energy is reduced; compared with an existing DBA type photoinitiator, the problem of alkoxy carbon-oxygen bond breakage caused by p-pi conjugation of DBA is avoided, generation of small molecule fragments is effectively inhibited, defects are reduced, and the yield is increased. In addition, compared with DPHA and DAcOA, the photosensitizer also shows better solubility and formula compatibility. The preparation method has the characteristics of simple preparation process and low cost, and can be widely applied to the photocuring fields of dry films, paints, coatings, printing inks, forming materials and the like.

The invention discloses a method and a device for detecting a watch plasma nano-film based on image processing, and the method comprises the steps: carrying out the dual-polarization light intensity data collection of the watch plasma nano-film through the photobleaching effect excitation processing and phase modulation analysis, and carrying out the detection of the dual-polarization light intensity data of the watch plasma nano-film; according to the method, a dynamic recovery feature and region segmentation technology is utilized, a point with the maximum photobleaching depth is selected as a reference datum for differential processing, accurate decoupling of a geometric curvature effect and a material effect is achieved, and therefore the intrinsic performance of the nano-film is accurately evaluated. According to the technical scheme, the problem of nonlinear interference caused by a watch curved surface can be effectively solved, and a reliable nano-film quality detection result is provided.

The invention provides a diepoxy alkoxy anthracene photosensitizer, and relates to the field of photopolymerization. The invention further comprises a light-cured resin composition containing the diepoxy alkoxy anthracene photosensitizer, a light-sensitive dry film containing the diepoxy alkoxy anthracene photosensitizer and application of the light-cured resin composition and the light-sensitive dry film. The photosensitizer provided by the invention has the characteristics of low migration before curing and low precipitation after curing, and is excellent in photosensitive property and high in photobleaching efficiency; the polymer can be widely applied to the fields of photopolymerization and photocuring of dry films, paints, coatings, printing ink, forming materials and the like; in a photosensitive dry film, the photosensitizer cannot migrate to a polyethylene film, and when an electroplating process is carried out after exposure and development, fragments cannot be separated out to pollute electroplating liquid, so that undesirable phenomena of short circuit, open circuit and the like of an anti-corrosion pattern are avoided, and the yield of a product is remarkably improved.

The invention discloses aggregation-induced emission microspheres with colorimetric-fluorescence double signals as well as a preparation method and application of the aggregation-induced emission microspheres. The aggregation-induced emission microsphere comprises a carboxyl polystyrene microsphere and an aggregation-induced emission dye embedded in the carboxyl polystyrene microsphere, the aggregation-induced emission microsphere has colorimetric-fluorescence double signals, is red under natural light, has strong absorption at 600nm, shows high luminous efficiency at 725nm, not only effectively avoids the interference of biological autofluorescence and reduces background interference, but also has good light stability and photobleaching resistance, and can be used for preparing the aggregation-induced emission microsphere. And due to the high molar extinction coefficient, the colorimetric and fluorescent double-signal output capability is realized at the same time. In addition, the aggregation-induced emission microspheres are high in reproducibility in the preparation process, have the potential of large-scale synthesis and meet the requirements of industrial batch production.

The invention provides a photosensitive resin composition containing an anthracene methyl quaternary ammonium salt photosensitizer and application of the photosensitive resin composition, a structural main body anthracene ring of the photosensitizer is introduced into methylene substituted by a quaternary ammonium group at a position 10 and / or a position 9, and an acid radical or halogen is adopted as an equilibrium anion; compared with a commercially available anthracene photosensitizer, the photosensitizer has excellent fat solubility and good water solubility through introduction of the quaternary ammonium salt on the premise that the photobleaching property is retained, great potential is brought to formula design of the photosensitive composition, and development waste in a later development process can be greatly reduced; the method can be widely applied to the photo-curing fields of dry films, paints, coatings, printing ink, forming materials and the like.

The invention discloses a high-precision fluorescence modulation super-resolution microscopic imaging method which is applied to the technical field of optical microscopic imaging. According to the novel high-precision fluorescence modulation super-resolution imaging technology, first-order Bessel annular light with a smaller point spread function is used for replacing common annular light generated by modulating Gaussian light through a vortex phase plate in the traditional technology, and the problem that the resolution ratio is insufficient in an existing fluorescence subtraction microscopy technology can be solved. The problem of image distortion caused by a negative side lobe is solved, the visibility and authenticity of a fine structure in an image are enhanced, and when any type of laser scanning confocal microscopy system and a fluorescence subtraction super-resolution imaging technology are oriented, only a wavefront control light path comprising a spatial light modulator and a Fourier lens needs to be added in an optical system, and the optical system can be used for controlling the optical system. Compared with the traditional super-resolution technology, the technology has the advantages that the complexity and cost of the system are reduced, the harm of photobleaching is reduced, the technology can be combined with other imaging technologies for use, and the imaging precision and the imaging quality are improved.

The invention discloses a colorimetric-fluorescent bimodal nanoprobe for detecting cMyBP-C and a micro-fluidic chip, and belongs to the technical field of biomedical detection and in-vitro diagnosis. The colorimetric-fluorescent bimodal nanoprobe which takes UCNPs as a core and MOF as a shell and is loaded with horse radish peroxidase and a labeled antibody is prepared by providing a layer-by-layer self-assembly technology; the micro-fluidic chip is prepared according to the probe, the micro-fluidic chip can realize cMyBP-C colorimetric and fluorescence signal bimodal detection, and the technical problems of limited sensitivity, susceptibility to background interference, limited linear range, photobleaching phenomenon, signal instability and the like in single signal detection are solved.

The invention belongs to the field of fluorescence detection, and particularly relates to a ratio-type fluorescent nanoprobe for specifically detecting tetracycline antibiotics as well as a preparation method and an application method of the ratio-type fluorescent nanoprobe. The nano-probe is a europium modified zinc gallium germanate nano-material, and is prepared by amino modification, citric acid modification and europium modification of the zinc gallium germanate nano-material. The probe can be specifically combined with tetracycline antibiotics; the fluorescent probe has two different fluorescence emission intensities, so that interference can be effectively eliminated, and the detection accuracy is high. And the synthesis method is simple and low in cost. The ratio type fluorescent nanoprobe provided by the invention also can realize the change of different colors under an ultraviolet lamp, can realize quantitative visual detection of tetracycline by combining the RGB value and the tetracycline concentration change, and can realize rapid on-site detection of tetracycline in a sample without the help of a fluorescent instrument. The tetracycline fluorescent probe is stable in optical performance, not prone to photobleaching, high in tetracycline recognition capacity, wide in response range (0.2-70 [mu] M) and low in detection limit, and has remarkable industrial application potential.

The invention provides a rare earth complex as well as a preparation method and application thereof. The chemical general formula of the rare earth complex is (Ln < 1x > Ln < 2y > Ln < 31-x-y >) (A) m (B) n, the rare earth complex provided by the invention has a self-assembly behavior, spherical nanoparticles can be formed through molecular aggregation, under ultraviolet irradiation, internal ligand molecules are better protected, the photobleaching process of the rare earth complex under natural conditions is slowed down, and the rare earth complex has better stability and higher weather resistance in the natural environment.

The present invention belongs to the technical field of functional food application. Disclosed in the present invention are a plant extract modified molecular probe and a preparation method and an application thereof. The preparation method of the plant extract modified molecular probe comprises the following steps: dropping a 3-ethyl-2-methylbenzothiazole iodide solution into a saffron aldehyde solution to obtain a mixed solution; dropping an inorganic salt solution into the mixed solution, mixing and performing a condensation reaction to obtain a plant extract modified molecular probe. The obtained plant extract modified molecular probe has good chemical stability and can be stored for a long time. It has good biocompatibility, is non-toxic, and has good photostability, it is not prone to photobleaching during long-term irradiation.

The invention discloses a microscopic imaging device and a super-resolution microscopic imaging method, and the device comprises a sample stage which is used for containing a sample; the laser is used for emitting laser; the first digital micromirror is used for receiving the laser and generating diffracted light; the light condensing unit is used for condensing the plurality of diffracted light to form structured light so as to excite fluorescence carrying sample information; the second digital micromirror is used for receiving the fluorescence and converting the fluorescence into a random binary coding image; the image acquisition unit is used for receiving images and transmitting the images to the processing unit; the processing unit is used for reconstructing a microscopic super-resolution image based on the plurality of random binary coding images; the first digital micromirror, the second digital micromirror and the image acquisition unit are in communication connection, and the second digital micromirror controls the grating pattern conversion frequency of the first digital micromirror and controls the image acquisition frequency of the image acquisition unit. The biological cell sample can be protected from being damaged by phototoxicity and photobleaching performance, and super-resolution imaging in the structured illumination obvious microsystem is achieved.

The invention discloses a single molecule positioning method based on high-speed phase-locked detection, which can perform high-precision single molecule positioning and imaging on a fluorescent sample under the condition of using a single laser light source. Molecular excitation of a sub-diffraction limit region is generated by using nonlinear characteristics of excitation and fluorescence emission when a fluorescence sample is excited by laser, and meanwhile, positioning information is obtained by surrounding a single molecule of a region of interest by using an illumination light beam; and the laser intensity is continuously improved, and the positioning precision is also improved until sub-five-nanometer precision information is obtained. And the detection end adopts a high-speed lock-in amplifier to lock fluorescence signal information of characteristic frequency in real time, and meanwhile, the final signal-to-background ratio is also improved. Compared with traditional high-precision positioning based on double laser light sources, the method has the advantages of a single laser light source, does not need special fluorescent dye, does not need special matching between double-wavelength lasers, and has universality; and the damage caused by phototoxicity and photobleaching due to over-high illumination intensity is avoided.

The invention discloses a tetraarylethene modified rhodamine-like derivative as well as a preparation method and application thereof. The rhodamine-like derivative has a structure as shown in a formula I, rhodamine is taken as a core skeleton, a tetraarylethene (TAE) structure with AIE characteristics is introduced into a meso site of the rhodamine, RL-TAE series molecules are obtained, the defect of aggregation-induced quenching (ACQ) is greatly improved through the strategy, the molar absorption coefficient is obviously increased, and the rhodamine-like derivative has a good application prospect. Therefore, a new scheme is provided for developing high-brightness aggregation-induced emission dye. The rhodamine-like derivative disclosed by the invention is simple in preparation method, mild in reaction condition and high in yield, has the characteristics of AIE effect, high molar absorption coefficient and brightness, photobleaching resistance and diamagnetism, is an excellent concentration-brightness correspondence dye, and can realize efficient coding application.

A biological aerosol monitoring apparatus, comprising a light path, a gas path and a signal processing system, wherein the light path comprises a laser irradiation light path configured to irradiate particles to be tested, a scattered light collection light path configured to receive scattered light signals, and a fluorescence collection light path configured to receive fluorescence signals. A focal point (M) of an excitation light source (101) configured to irradiate said particles is located before or behind a photosensitive area (G) on an optical axis of the laser irradiation light path, such that the cross section of an excitation light spot in the photosensitive area (G) that is perpendicular to the optical axis of the laser irradiation light path is a rectangular shape, and when a single aerosol particle passes through the photosensitive area (G), the single aerosol particle is continuously irradiated by excitation light to obtain an intrinsic fluorescence bleaching speed. Information such as an intrinsic fluorescence bleaching speed, a scattered light peak value and a relative fluorescence intensity of aerosol particles is integrated, such that preliminary classification of biological particles is realized, instrument alerts caused by non-target microorganisms are significantly reduced, and the capability of an instrument for identifying the types of biological aerosols is improved, thereby reducing the frequency and cost of subsequent biological detection.

The invention belongs to the technical field of industrial silane preparation, and provides a natural silane rotor and a preparation method and application thereof.The preparation method comprises the steps that a pyridoxal phosphate solution and a 4-amino ethyl cinnamate solution are mixed and then react to obtain pyridoxal phosphate cinnamyl ester; and mixing the pyridoxal phosphate cinnamyl ester solution and the amino silane solution, and reacting to obtain the natural silane rotor. The silane rotor has a good conjugated structure, is stable in chemical structure, can be stored for a long time, is good in light stability, and is not liable to cause a photobleaching phenomenon. Besides, conjugated single and double bonds capable of freely rotating exist in the molecular structure, a typical flexible intramolecular rotation characteristic is presented, the conjugated structure can freely rotate in a dilute solution, a surface sightseeing signal is weak, the free rotation phenomenon is limited in a thick solution, the surface sightseeing signal is strong, and the surface sightseeing signal is strong. Therefore, the device can be used as a molecular tool for monitoring the change of the thin consistency of the silane, and can provide real-time, lossless and visual detection support for the development of a silane modulation process.

The invention relates to the technical field of fluorescence imaging, in particular to a derivative of triphenylamine and thiazole salt, a fluorescent probe as well as a preparation method and application of the fluorescent probe. The fluorescent probe disclosed by the invention is a derivative of triphenylamine and thiazole salt. The structure of the fluorescent probe contains a positive charge, and the internal environment of the mitochondria is a negative charge. When the fluorescent probe provided by the invention is used in cell imaging, the fluorescent probe can penetrate through a cell membrane to enter living cells and is adsorbed on mitochondria under the attraction of negative charges in the mitochondria. In the state, after the fluorescent probe is excited to generate fluorescence reaction based on a specific light field, fluorescence imaging of the cells can be realized, so that the internal change process of mitochondria can be observed through real-time fluorescence imaging. The fluorescent probe disclosed by the invention has relatively small cytotoxicity and relatively good photobleaching resistance, can specifically target mitochondria and can realize long-time fluorescence imaging, so that the fluorescent probe disclosed by the invention has relatively good advantages in cell imaging.

The invention provides fluorescent carbon dots and fluorescent hydrogel based on solvent stimulation response discoloration and a preparation method and application of the fluorescent carbon dots and the fluorescent hydrogel, and belongs to the technical field of fluorescent materials and information encryption. The preparation method of the fluorescent carbon dots comprises the following steps: mixing a biomass carbon source and a solvent, and performing solvothermal reaction to obtain the fluorescent carbon dots, the biomass carbon source comprises one or more of buxus buxus, oriental cherry leaves, fructus forsythiae leaves and oak leaves; the solvent is ethanol. The biomass carbon dots are low in toxicity, excellent in optical stability and photobleaching resistance and high in ionic strength; the fluorescent carbon dots have double emission peaks of red light and blue light / green light, are different from single fluorescent carbon dots, and can provide more fluorescent information storage density for a fluorescent gel-based information orientation encryption system. The fluorescent color of the fluorescent hydrogel can be stably switched in an ethanol / water solvent, the gel structure is stable, the information storage capacity is improved, and a fluorescent hydrogel-based information anti-counterfeiting encryption system is promoted to develop towards multiple dimensions.

The present invention provides a photosensitive resin composition containing a photo-bleachable aminomethyl anthracene photosensitizer and its applications. An aminomethyl functional group is introduced at the 9- and / or 10-positions of the anthracene ring, which is the main structure of the photosensitizer. By introducing the aminomethyl group, while maintaining the electron cloud density of the polycyclic aromatic hydrocarbon, the maximum absorption wavelength is red-shifted, especially suitable for the 405 nm light irradiation system, significantly improving the initiation efficiency and reducing the exposure energy. Compared with the existing DBA-type photoinitiators, the problem of the cleavage of the alkoxy carbon-oxygen bond caused by p-π conjugation in DBA is avoided, effectively inhibiting the generation of small molecule fragments, reducing defects and improving the yield. Compared with DPHA and DAcOA, this photosensitizer also exhibits better solubility and formulation compatibility. The present invention has the characteristics of simple preparation process and low cost, and can be widely applied to the field of photocuring such as dry films, paints, coatings, inks and molding materials.

Provided herein are fluorescently-labeled nucleotide conjugates for nucleic acid analysis. Also provided are reagents used for forming binding complexes between a fluorescently-labeled nucleotide conjugate and a target nucleic acid sequence in the presence of one or more reagents disclosed herein. Binding complexes can be detected in the presence of the one or more reagents. For example, the one or more reagents may contain a photobleaching reducing agent configured to reduce photobleaching resulting from use of the fluorescently-labeled nucleotide conjugate to form the binding complex in a nucleic acid analysis. Such nucleic acid analysis may be used to identify sites of nucleobase binding or incorporation between the target nucleic acid sequence and one or more nucleotide moieties of the fluorescently-labeled nucleotide conjugate in a nucleic acid sequence reaction.

Decoding methods are provided for identifying populations in assays, particularly multiplexed assays and those associated with fluidic devices. Populations of beads are encoded with different signals, such as dyes with different photostabilities. Changes, such as photobleaching, allow for differentiation of different populations in kinetic assays that measure fluorescence decay.

The invention discloses a preparation method and application of a red fluorescent copper nano-cluster. According to the preparation method, a red fluorescent copper nano-cluster aqueous solution is prepared at room temperature by using polyvinylpyrrolidone as a protective agent, 3-mercaptophenylboronic acid as a stabilizer and a reducing agent and water-soluble copper salt as a copper source. The preparation method has the advantages of being simple in preparation process, low in instrument requirement, short in treatment time, low in cost and the like, the fluorescence quantum yield of the prepared red fluorescent copper nano-cluster is 4.9%, and the prepared red fluorescent copper nano-cluster has the advantages of being good in water solubility, good in stability, high in photobleaching resistance, high in salt resistance, long in fluorescence service life and the like, has high sensitivity and selectivity to Congo red, and is suitable for industrial production. The method can be applied to detection of fluorescence quenching type Congo red.

Method for single molecule localization microscopy (SMLM) based on conventional fluorescent binder probes, including primary or secondary antibodies, that are applied in a gradual stepwise fashion to a fixed biological sample. In each step of the process, a low dose of the probe is applied in solution to the fixed biological sample to sparsely label target epitopes followed by sample washing to remove the free probe. The remaining bound fluorescent probes that are sparsely distributed over the sample are then imaged until they are photobleached. The high light collection efficiency from each probe in this approach (limited only by photobleaching) enables few Ångstrom lateral resolution and few nm axial resolution. Photobleaching of the probes at the end of each step generates a clean slate for application of the next step in the process, as well as the possibility for applying one or more probes in a consecutive fashion to the same sample for serial SMLM.

The embodiment of the invention relates to an MOF (Metal Organic Framework) material, a preparation method thereof, acid-loaded inorganic salt sol and peroxide explosive detection application, and belongs to the technical field of fluorescence detection materials. The preparation method of the acid-loaded inorganic salt sol capable of cyclically detecting the peroxide explosive comprises the following steps: putting an MOF material and a film dispersion polymer into a solvent, so that the film dispersion polymer wraps the MOF material, and uniformly mixing to obtain gel; and adding acid into the gel, and reacting to load the acid on the gel to obtain the acid-loaded inorganic salt sol. The acid-loaded inorganic salt sol in the embodiment of the invention shows extremely high sensitivity, and the lowest detection limit can reach 10 nanograms (taking TATP as an example); in addition, the material shows excellent performance of resisting photooxidation quenching (namely resisting photobleaching) in a test environment, and can be quickly self-healed; even if the material is continuously tested for more than 500 times, the material can still keep activity and can be recycled without being damaged.

The invention relates to the technical field of nano materials and biomedical engineering, and discloses a dye-sensitized rare earth up-conversion luminescent nano material as well as a preparation method and application thereof. The dye-sensitized rare earth up-conversion luminescent nano-material comprises a rare earth doped up-conversion nano-particle core, an unsaturated fatty acid salt self-assembled shell layer and a near-infrared dye modified on the surface of the rare earth doped up-conversion nano-particle. The unsaturated fatty acid salt coating layer endows the material with excellent water dispersibility through a hydrophilic group; a hydrophobic chain segment and an unsaturated double bond can effectively block water and oxygen permeation and quench singlet oxygen, so that the photobleaching half-life period of the material is increased by 86.7 times or more compared with that of a conventional coating material, and the light stability is excellent; the material successfully realizes efficient up-conversion luminescence under excitation of a low-power near-infrared light emitting diode (NIR LED, the power density of which can be as low as 100mW / cm < 2 >), and thoroughly gets rid of dependence on a high-cost and high-risk laser.

This invention relates to aggregation-induced emission probes with specific responses to tumor markers and their applications, belonging to the field of biochemistry. This invention utilizes molecular structure modulation to prepare a series of molecules possessing large Stokes shifts, resistance to photobleaching, near-infrared II (NIR-II) emission, and aggregation-induced emission properties. Different self-eliminating groups are attached to the dimethylamino group of the molecule via quaternization or to the pyridine group via pyridinium salting. By controlling the structure of these self-eliminating groups, activatable luminescent probes responding to different tumor markers can be constructed. These probes integrate deep NIR-II imaging, aggregation-point luminescence, large Stokes shifts, and marker-specific responses, providing a new approach for precise imaging diagnosis of tumors.

The application discloses an aggregation-induced emission compound with double fluorescence emission characteristics and a preparation method and application thereof, and the proportional fluorescence probe has a structure of general formula I. The probe molecule has a polar response group tetraphenyl ethylene as a fluorescence report unit, introduces a hydrophilic oxygen atom, connects an alpha carbon of an acetamide, and connects a polar detection sensitization group Tag chain on an amino group of the acetamide. The probe molecule improves the conjugation degree of oxygen atom lone pair electrons and a tetraphenyl ethylene pi electron system, so that the probe molecule simultaneously produces n→pi* and pi→pi* molecular transitions, and a proportional fluorescence probe which can sensitively respond to polarity change is constructed. The fluorescence probe designed in the application has the advantages that the fluorescence probe is not easily interfered by probe concentration, a detection environment and light bleaching, and experiments prove that the proportional fluorescence intensity of the fluorescence probe can sensitively respond to polarity, and the proportional fluorescence intensity and water content are linearly fitted, so that the purposes of detecting water content in an organic reagent and alcohol content in wine are achieved.

The invention discloses a novel supramolecular cyanine dye contrast agent for enhancing photoacoustic, and a preparation method and application thereof. Wherein the molecular pair cyanine dye is composed of a cationic indocyanine dye and an anionic tricyanofurancyanine dye. The dye is subjected to J-aggregation in water, so that the absorption wavelength is subjected to red shift to a near-infrared region, and excellent photothermal conversion efficiency (10-80%) and singlet oxygen quantum yield (10-50%) are shown. The photoacoustic imaging performance is excellent, and the intensity of a photoacoustic signal generated under the concentration of 25.0 mu M is 7.1 times that of ICG; the photo-thermal treatment effect is obvious, the photo-thermal conversion efficiency is as high as 66%, and the temperature is rapidly increased to 48 DEG C. In addition, the dye shows excellent light stability and photobleaching resistance, the absorbance is only attenuated by 25% under strong laser, and the dye has good biocompatibility and safety. The multi-mode application comprises photoacoustic and fluorescence imaging, photothermal and photodynamic therapy and the like, and has a good application prospect.

The application discloses an organic compound biological probe and a preparation method and application thereof, and belongs to the field of fluorescent probes. The structural formula of the organic compound biological probe is as follows, and the preparation method comprises the following steps: adding 2,4-dihydroxybenzaldehyde and anhydrous potassium carbonate into anhydrous acetonitrile or DMF, then dropwise adding 1,4-dibromobutane to obtain a colorless oily compound 1 through reflux reaction; dissolving the compound 1 and triphenylphosphine in anhydrous acetonitrile to obtain a solid compound 2 through reflux reaction; dissolving 1,1,2-trimethyl-1H-benzo[e]indole and iodoethane in anhydrous acetonitrile to obtain a compound 3 through reflux; dissolving the compound 3 and the compound 2 in anhydrous ethanol, adding piperidine, and then performing reflux reaction and purification to obtain the organic compound biological probe. The organic compound biological probe can self-assemble into nanoparticles with a size of about 1.6 nm in water, has strong anti-photobleaching ability, and integrates red light and near-infrared dual-channel imaging, pH detection, mitochondrion targeting and chemotherapy.

The application discloses an aggregation-induced emission microsphere with colorimetric-fluorescent double signals and a preparation method and application thereof. The aggregation-induced emission microsphere comprises carboxyl polystyrene microspheres and aggregation-induced emission dyes embedded in the carboxyl polystyrene microspheres; and the structural formula of the aggregation-induced emission dyes is as follows: The aggregation-induced emission microsphere has colorimetric-fluorescent double signals, is red under natural light, has strong absorption at 600 nm, and shows high light-emitting efficiency at 725 nm, effectively avoids the interference of biological autofluorescence, reduces background interference, has good light stability and light bleaching resistance, and has high molar extinction coefficient, so that the aggregation-induced emission microsphere has colorimetric and fluorescent double signal output capabilities. In addition, the preparation process of the aggregation-induced emission microsphere has high reproducibility, has the potential for large-scale synthesis, and meets the needs of industrial batch production.

The invention discloses a photobleached all-polymer optical waveguide grating sensor and a preparation method thereof, and belongs to the technical field of polymer optical waveguide grating sensors. The preparation method comprises the following steps: carrying out hot stamping on a substrate sheet to obtain a substrate sheet with a groove; spin-coating photoresist on the substrate sheet to form a photoresist core layer; photoetching the photoresist core layer through double-beam interference to obtain the photoresist core layer with interference fringes; spin-coating a thermosensitive polymer cladding material on the photoresist core layer, covering the flexible substrate and then carrying out low-temperature thermocuring so as to bond the photoresist core layer and the flexible substrate; stripping the substrate sheet, exposing the photoresist core layer, covering the protective cladding, and curing to obtain a multi-layer composite device; and performing non-uniform post-baking on the multi-layer composite device to obtain the multi-layer composite device. According to the invention, the grating period can be changed under the condition that the grating mask is not replaced, and the cost of spectrum adjustment is greatly reduced.