96 results about "Photobleaching" patented technology

The invention provides a photosensitive resin composition containing an anthracene methyl quaternary ammonium salt photosensitizer and application of the photosensitive resin composition, a structural main body anthracene ring of the photosensitizer is introduced into methylene substituted by a quaternary ammonium group at a position 10 and / or a position 9, and an acid radical or halogen is adopted as an equilibrium anion; compared with a commercially available anthracene photosensitizer, the photosensitizer has excellent fat solubility and good water solubility through introduction of the quaternary ammonium salt on the premise that the photobleaching property is retained, great potential is brought to formula design of the photosensitive composition, and development waste in a later development process can be greatly reduced; the method can be widely applied to the photo-curing fields of dry films, paints, coatings, printing ink, forming materials and the like.

The invention relates to the technical field of nano materials and biomedical engineering, and discloses a dye-sensitized rare earth up-conversion luminescent nano material as well as a preparation method and application thereof. The dye-sensitized rare earth up-conversion luminescent nano-material comprises a rare earth doped up-conversion nano-particle core, an unsaturated fatty acid salt self-assembled shell layer and a near-infrared dye modified on the surface of the rare earth doped up-conversion nano-particle. The unsaturated fatty acid salt coating layer endows the material with excellent water dispersibility through a hydrophilic group; a hydrophobic chain segment and an unsaturated double bond can effectively block water and oxygen permeation and quench singlet oxygen, so that the photobleaching half-life period of the material is increased by 86.7 times or more compared with that of a conventional coating material, and the light stability is excellent; the material successfully realizes efficient up-conversion luminescence under excitation of a low-power near-infrared light emitting diode (NIR LED, the power density of which can be as low as 100mW / cm < 2 >), and thoroughly gets rid of dependence on a high-cost and high-risk laser.

This invention relates to aggregation-induced emission probes with specific responses to tumor markers and their applications, belonging to the field of biochemistry. This invention utilizes molecular structure modulation to prepare a series of molecules possessing large Stokes shifts, resistance to photobleaching, near-infrared II (NIR-II) emission, and aggregation-induced emission properties. Different self-eliminating groups are attached to the dimethylamino group of the molecule via quaternization or to the pyridine group via pyridinium salting. By controlling the structure of these self-eliminating groups, activatable luminescent probes responding to different tumor markers can be constructed. These probes integrate deep NIR-II imaging, aggregation-point luminescence, large Stokes shifts, and marker-specific responses, providing a new approach for precise imaging diagnosis of tumors.

The application discloses an aggregation-induced emission compound with double fluorescence emission characteristics and a preparation method and application thereof, and the proportional fluorescence probe has a structure of general formula I. The probe molecule has a polar response group tetraphenyl ethylene as a fluorescence report unit, introduces a hydrophilic oxygen atom, connects an alpha carbon of an acetamide, and connects a polar detection sensitization group Tag chain on an amino group of the acetamide. The probe molecule improves the conjugation degree of oxygen atom lone pair electrons and a tetraphenyl ethylene pi electron system, so that the probe molecule simultaneously produces n→pi* and pi→pi* molecular transitions, and a proportional fluorescence probe which can sensitively respond to polarity change is constructed. The fluorescence probe designed in the application has the advantages that the fluorescence probe is not easily interfered by probe concentration, a detection environment and light bleaching, and experiments prove that the proportional fluorescence intensity of the fluorescence probe can sensitively respond to polarity, and the proportional fluorescence intensity and water content are linearly fitted, so that the purposes of detecting water content in an organic reagent and alcohol content in wine are achieved.

The application discloses an aggregation-induced emission microsphere with colorimetric-fluorescent double signals and a preparation method and application thereof. The aggregation-induced emission microsphere comprises carboxyl polystyrene microspheres and aggregation-induced emission dyes embedded in the carboxyl polystyrene microspheres; and the structural formula of the aggregation-induced emission dyes is as follows: The aggregation-induced emission microsphere has colorimetric-fluorescent double signals, is red under natural light, has strong absorption at 600 nm, and shows high light-emitting efficiency at 725 nm, effectively avoids the interference of biological autofluorescence, reduces background interference, has good light stability and light bleaching resistance, and has high molar extinction coefficient, so that the aggregation-induced emission microsphere has colorimetric and fluorescent double signal output capabilities. In addition, the preparation process of the aggregation-induced emission microsphere has high reproducibility, has the potential for large-scale synthesis, and meets the needs of industrial batch production.

The invention discloses a photobleached all-polymer optical waveguide grating sensor and a preparation method thereof, and belongs to the technical field of polymer optical waveguide grating sensors. The preparation method comprises the following steps: carrying out hot stamping on a substrate sheet to obtain a substrate sheet with a groove; spin-coating photoresist on the substrate sheet to form a photoresist core layer; photoetching the photoresist core layer through double-beam interference to obtain the photoresist core layer with interference fringes; spin-coating a thermosensitive polymer cladding material on the photoresist core layer, covering the flexible substrate and then carrying out low-temperature thermocuring so as to bond the photoresist core layer and the flexible substrate; stripping the substrate sheet, exposing the photoresist core layer, covering the protective cladding, and curing to obtain a multi-layer composite device; and performing non-uniform post-baking on the multi-layer composite device to obtain the multi-layer composite device. According to the invention, the grating period can be changed under the condition that the grating mask is not replaced, and the cost of spectrum adjustment is greatly reduced.

The invention provides a method for detecting fusion of extracellular vesicles by adopting fluorescence resonance energy transfer, and belongs to the technical field of extracellular vesicles. A dynamic fluorescence monitoring system is established, low-power laser excitation is adopted, dual-wavelength fluorescence signals are monitored at the same time, fluorescence signal data are collected, graded gain regulation and control processing is carried out through five threshold values, and a fluorescence signal super-sparse matrix is constructed; establishing a fluorescence signal processing matrix model, converting the super-sparse matrix into a dense matrix, constructing a gain matrix for signal correction, calculating a fluorescence resonance energy transfer efficiency parameter, obtaining a standardized efficiency value through matrix operation, outputting an extracellular vesicle fusion kinetic analysis result, and establishing a quantitative evaluation system. The technical problems of poor signal stability and low detection precision in the process of fluorescence resonance energy transfer detection of extracellular vesicle fusion are solved.

This invention discloses an illumination intensity modulation method for disk scanning confocal imaging, comprising the following steps: S1: Before formal imaging, a pre-imaging is performed using weak, uniform illumination; S2: The fluorescence intensity of different regions in the pre-imaging is evaluated and analyzed, and the evaluation results are obtained; S3: Based on the evaluation results, the parameter settings of the intensity modulation mechanism are adjusted to achieve different illumination in different regions of the sample; S4: The scanning points traverse the imaging area to obtain a complete image on the camera. This invention can dynamically adjust the illumination intensity of local regions in the scanning imaging as needed, and can be used to reduce photobleaching in local regions, increase the dynamic range of imaging, and enhance the signal-to-noise ratio of local regions.

The application discloses a pyridine cation mitochondrial fluorescent probe, the chemical name of the probe is 4-(8-dimethylamino-5-benzocoumarin)-methylpyridine iodine salt, and the chemical structural formula is shown as formula (I). The application further discloses application of the fluorescent probe in labeling or imaging display of mitochondrial distribution or morphology in living cells and in long-range tracking display of mitochondria in living cells and imaging display of nucleoli in cells. Experiments prove that the labeled mitochondrial fluorescent probe has high light bleaching resistance and a high fluorescence quantum yield, can realize long-range tracking of mitochondria in living cells, and can label fixed nucleoli in cells. It is indicated that the fluorescent probe has potential application and development value in the fields of fluorescent probes and tissue imaging, and has a wide application prospect.

The application provides a photosensitive resin composition containing an anthracene methyl quaternary ammonium salt photosensitizer and application thereof, the photosensitizer has a quaternary ammonium group substituted methylene introduced at 10 and / or 9 positions of the anthracene ring, and an acid radical or halogen is used as a counter anion; compared with a commercially available anthracene photosensitizer, the photosensitizer has excellent fat solubility and good water solubility by introducing the quaternary ammonium salt on the premise of retaining photobleaching, brings great potential for formula design of the photosensitive composition, and can greatly reduce developing waste in a later developing process; and can be widely applied in the fields of dry films, paints, coatings, inks and molding materials and the like.

The invention discloses a nitrogen-doped carbon dot and a rapid fluorescence detection method thereof for indoxacarb residue, and belongs to the field of biosensor technology and food quality detection. L-theanine and ethidene diamine are used as raw materials, the nitrogen-doped carbon dots are prepared through a hydrothermal method, the N-CDs have the advantages of being stable in fluorescence emission, good in salt tolerance and excellent in photobleaching resistance, and indoxacarb can specifically quench fluorescence through the static quenching effect. By optimizing N-CDs synthesis conditions and indoxacarb detection conditions, an indoxacarb fluorescence sensing detection system with high selectivity and high sensitivity is constructed. The detection method disclosed by the invention can be suitable for detecting indoxacarb residues in environmental water samples such as tap water and river water and agricultural product matrixes such as apples and tea leaves, the standard recovery rate is good, the relative standard deviation is low, on-site rapid screening of the indoxacarb residues can be realized, and a new technical means is provided for monitoring the quality safety of the environment and agricultural products.

The anti-photobleaching excitation light-induced fluorescence heavy metal detection system comprises a system control unit, a fluorescence excitation unit, a fluorescence detection unit and a data acquisition card; the system control unit sends a pulse excitation signal to make the fluorescence excitation unit generate pulse fluorescence; the fluorescence detection unit collects the pulse fluorescence and converts it into an electric signal input into the data acquisition card; the system control unit sends a fluorescence detection signal to make the data acquisition card save n fluorescence intensity values; and the data acquisition card averages the n fluorescence intensity values. The application avoids the problem of photobleaching of the fluorescence probe caused by long-time irradiation of the excitation light by shortening the irradiation time of the excitation light, reduces the error of the detection result and improves the service life of the fluorescence probe.

Provided is a method of screening for an autophagy modulator. According to the screening method of an aspect, the autophagy modulator can be accurately screened with only a single cell. In addition, screening can be performed without using fluorescent labeling, and thus cytotoxicity and photobleaching that occur when fluorescent labeling analysis methods are used can be prevented, and the limitation of having to monitor only fluorescently labeled markers can be improved.

The invention provides anthracene methyl thiosulfonate for a photosensitive resin composition, and relates to the field of photopolymerization. The invention also comprises a photosensitive resin composition containing the anthracene methyl thiosulfonate, a photosensitive dry film containing the anthracene methyl thiosulfonate, and applications of the photosensitive resin composition and the photosensitive dry film. The anthracene methyl thiosulfonate provided by the invention has photosensitivity, can be used as a photosensitizer in a photosensitive resin composition, can improve the adhesive force of the photosensitive resin composition on various metal surfaces, has the characteristic of low migration, has good compatibility with monomers and solvents, and can be widely applied to the photocuring fields of dry films, paints, coatings, inks, molding materials and the like; and the photobleaching agent has extremely high photobleaching efficiency and is particularly suitable for being used in a formula of an ultra-thick film.

The invention discloses a micro / nano plastic based on organic long-afterglow material labeling and a preparation method and application thereof, and belongs to the technical field of crossing of environmental science and macromolecular nano materials. According to the method, organic long-afterglow material dispersion liquid is dropwise added into micro / nano plastic dispersion liquid, and swelling is balanced; and carrying out rotary evaporation to remove the swelling agent, washing, and dialyzing until the dialysate has no obvious ultraviolet absorption, so as to obtain the micro / nano plastic marked by the organic long afterglow material. According to the method, the characteristic that the organic long-afterglow material can realize high-brightness luminescence without in-situ excitation is utilized, and the background interference of an environmental medium is effectively inhibited, so that the signal-to-noise ratio and the sensitivity of micro / nano plastic detection are remarkably improved under the environmental concentration condition. Compared with an existing marking technology, the method has the advantages that the operation is simple, the cost is low, the biocompatibility is good, and the problems of false positive interference and photobleaching can be effectively avoided; good application prospects are shown in the fields of environment migration behavior research of micro / nano plastic, bioluminescence imaging and the like.

Disclosed herein are compositions, systems, kits, and methods for photobleaching, and selective photobleaching, a biological sample.

The present application relates to a kind of metal nanoparticle-based plasmonic enhanced fluorescence multilayer film optical structure and its preparation method and application, the multilayer film optical structure is by with self-assembly metal nanoparticle as enhancement substrate, to be measured fluorescent probe molecule is placed on the surface of the enhancement substrate and obtains.The present application is by the surface plasmon resonance of self-assembly metal nanoparticle, while effectively inhibiting fluorescent probe molecule photobleaching, greatly improves the sensitivity of fluorescence trace gas sensing, the service life of organic probe fluorescence and gas sensor reliability;Compared with the prior art glass substrate fluorescent film technology, the performance of the present application is comprehensively improved, and has good market application prospect.

The present invention belongs to the technical field of biomedical detection and in-vitro diagnosis. Disclosed are a colorimetric-fluorescent dual-mode nanoprobe and microfluidic chip for detecting cMyBP-C. In the present invention, a colorimetric-fluorescent dual-mode nanoprobe, which comprises UCNPs as a core and MOF as a shell and is loaded with horseradish peroxidase and a labeled antibody, is prepared by means of a "layer-by-layer self-assembly" technique; and then a microfluidic chip is prepared on the basis of the nanoprobe. The microfluidic chip realizes dual-mode detection of colorimetric and fluorescent signals of cMyBP-C, thereby solving technical problems such as limited sensitivity, susceptibility to background interference, limited linear range, photobleaching and signal instability in single-signal detection.

A method of forming a multicolour light emitting array, the method comprising: providing a first light emitting device configured to emit light with a first primary peak wavelength and a second light emitting device configured to emit light with the first primary peak wavelength; forming a colour conversion region at least partially associated with the first light emitting device and the second light emitting device, wherein the colour conversion region is configured to absorb light with the first primary peak wavelength and emit light with a second primary peak wavelength longer than the first primary peak wavelength; and photo-bleaching a portion of the colour conversion region associated with the first light emitting device such that the colour conversion region associated with the first light emitting device is at least partially transparent to light with the first primary peak wavelength, thereby to enable light with the first primary peak wavelength to be emitted by a first pixel associated with the first light emitting device and light with the second primary peak wavelength to be emitted by a second pixel associated with the second light emitting device.

The invention discloses an application of an aggregation-induced emission (AIE) photosensitizer in preparation of a drug-resistant bacterium resisting product. The AIE photosensitizer disclosed by the invention has high active oxygen generation efficiency and can be applied to preparation of anti-drug-resistant bacteria type fibers. The AIE photosensitizer and the fiber prepared from the AIE photosensitizer have strong killing activity on a plurality of pathogenic microorganisms including drug-resistant gram-negative bacteria, gram-positive bacteria and the like. In addition, the pathogens can be efficiently and rapidly killed under the illumination of white light with extremely low power density; and even under the condition that illumination is not added, a good killing effect is also achieved on pathogens. Meanwhile, the AIE photosensitizer disclosed by the invention has good photobleaching resistance, good biocompatibility and pathogen capturing capacity, and can be applied to textile raw materials, medical materials and the like.

The invention discloses a multi-target fluorescence imaging method which comprises the following steps: S1, exciting each sample by using single-wavelength exciting light with the same power, and continuously collecting fluorescence images to obtain a fluorescence image sequence; s2, extracting a time-varying curve of the total fluorescence signal intensity of the fluorescent protein from each fluorescence image sequence, and performing exponential decay fitting on each fluorescence-time variation curve; and S3, obtaining photobleaching characteristic parameters of the four fluorescent proteins according to the fitting equation, and generating a single fluorescent image of each target from multi-target sample imaging marked by the four fluorescent proteins. The method comprises the following steps: marking targets by adopting fluorescent proteins with the same or similar emission wavelengths, carrying out fluorescence imaging acquisition under the same exciting light condition to obtain a photobleaching curve and bleaching characteristic parameters of each fluorescent protein, and accurately identifying a plurality of targets by taking photobleaching kinetic parameters as new judgment dimensions. And the chromatic aberration problem caused by wavelength difference is fundamentally eliminated.

This invention discloses an in-situ tyrosinase sensor based on a carbon quantum dot microneedle composite structure, its preparation method, and its application. Carbon quantum dots (Cate-CQDs) with surface-modified catechol groups were synthesized using urea, anhydrous citric acid, and catechol as raw materials. The Cate-CQDs were uniformly dispersed in a hydrogel and then fixed inside the microneedles after drying. When the microneedles pierce the target skin containing TYR, due to the polyphenol oxidase properties of TYR, catechol is specifically oxidized to benzoquinone by TYR, quenching the original fluorescence of the Cate-CQDs. This invention uses Cate-CQDs as a probe, possessing sensitive tyrosinase detection capabilities and resisting interference from common amino acids and proteases. Compared to small molecule fluorescent probes, carbon quantum dots exhibit stronger resistance to photobleaching. With a fluorescence detection limit as low as 0.052 U / mL and a colorimetric detection limit of 0.10 U / mL, and a linear range covering 0.5–100 U / mL, it can complete the detection within 30 minutes and is suitable for rapid analysis of tyrosinase concentrations in the skin or other matrices.

The application discloses a fluorescent coupling reaction low-light exposure control incubation integrated system, relates to the technical field of biological detection and experimental equipment, and comprises an operation dark box module, a sample incubation module, an imaging assembly and a main control module. The operation dark box is combined with non-visible light imaging and hand action detection, realizes low-light blind operation visualization and dynamic light adjustment, and reduces initial light exposure. The sample incubation module initiatively creates a grading light shielding system, uses local light shielding to timely stop loss of a single sample, and uses overall light shielding to unify the dark field environment of subsequent reactions of all batches. In addition, the system calculates accurate exposure time through operation triggering and in-situ detection, combines multi-level threshold value quantization to identify the light bleaching degree of samples, and automatically determines to discard when the time is up. The application effectively solves the problems of uncontrollable light exposure and uneven light receiving in the preparation and transfer process of large quantities of fluorescent samples, and guarantees the accuracy and high consistency of final fluorescent detection results.

A homogeneous laser illumination system for microscopy applications, based on the use of multimode optical fibers (MMF), is described. The system's operating principle relies on the superposition of dynamic speckle patterns generated from several multimode fibers. These patterns are created by applying random, high-frequency perturbations (tens of kilohertz) induced by a piezoelectric actuator that perturbs the optical fibers. The superposition of these patterns reduces illumination contrast, achieving a uniform light distribution across the field of view (FOV). The system's modularity and adaptability allow for its integration into existing microscopy setups. Furthermore, it can be configured to operate with standard commercial objective lenses, including immersion lenses, and adjusted to reduce photobleaching in fluorescent samples.The system uses multimode optical fibers and requires no additional infrastructure to manage vibrations or mechanical noise. It is suitable for fluorescence microscopy and has been successfully tested with wavelengths across the visible spectrum. The system enhances the illuminated area and optimizes the generation of homogeneous light patterns, providing a high-performance and efficient solution for obtaining high-quality images in microscopic studies.

The invention discloses a preparation method of a dissolved oxygen sensitive film, and belongs to the technical field of gas sensing and optical detection. The problems that in an existing optical dissolved oxygen sensitive film, dye is prone to photobleaching and gathering self-quenching, and the oxygen diffusion rate and dye protection are difficult to consider at the same time are solved. The method comprises the following steps: 1, preparing polystyrene nano / micron particle powder; and 2, preparing the oxygen sensitive film. The method is used for preparing the dissolved oxygen sensitive film.

Disclosed herein are compositions, systems, kits, and methods for photobleaching, and selective photobleaching, a biological sample.

The application discloses a near-infrared heptamethine cyanine dye and application thereof in the field of photothermal therapy. 2000 After being wrapped into nanoparticles, the photothermal stability of the heptamethine cyanine dye is greatly enhanced, no photobleaching occurs in four photothermal cycle experiments, the heptamethine cyanine dye has high efficient photothermal conversion capacity, and the photothermal conversion efficiency is as high as 82%. The photothermal reagent prepared from the heptamethine cyanine dye has significant cell killing capacity at low concentration and low light dose under light excitation, and has low dark toxicity. The near-infrared heptamethine cyanine dye provided by the application has simple structure, simple synthesis mode and excellent clinical application potential.

The invention relates to the technical field of chemical genetics marking, in particular to a marking probe for quantifying protein dynamics. The probe comprises a compound with the following structural formula, wherein the compound contains a halogenated alkane chain and can be specifically covalently bound with halogenated alkane dehalogenase-HaloTag tag protein. The HaloTag-containing fusion target protein is combined with the HaloTag-containing fusion target protein in cells, and has the advantages of high specificity and stability, low toxicity, good biocompatibility and strong photobleaching resistance in living cell imaging. The compound has the property of space-limited emission effect, and can sensitively mark and respond to the spatial dynamic change of any single protein molecule in the physiological process of cells through a HaloTag bridge, so that the dynamic quantification and visualization of the protein are realized in the method, and the compound is used for recording physiological activities under different time scales.