290 results about "Photobleaching" patented technology

Provided is a method of using a complex salt as anti-spotting detergents, the complex salt being formed by the reaction of a photo-bleaching component having a water-soluble anionic substituent and a cationic surfactant. The complex salt is water insoluble in a stationary state, such as hand washing or pre-soaking for machine washing, so it effectively suppress the spotting of the photo-bleaching component into the interwoven webs of fabric. In addition, the complex salt can uniformly and rapidly dissolve in an agitating state, such as when machine washing, to allow the photo-bleaching component to absorb into fabric for effective bleaching and enhanced detergency.

Refractive index of biological specimens is a source of intrinsic contrast that can be explored without any concerns of photobleaching or harmful effects caused by extra contrast agents. This feature also contains rich information that can be related to the metabolism of cells at the cellular and subcellular levels. The present invention relates to systems and methods that can provide, without any moving parts, the 3-D refractive index map of continuously flowing biological samples in a micro-fluidic channel, for example.

The present invention provides a formulation for a reactive dye layer for a limited-play optical storage medium, the reactive formulation including at least one carrier material or curable acrylate monomer, at least one reactive material disposed within the carrier material or acrylate monomer, and at least one photo-bleaching retarder material disposed within the at least one adhesive material. The at least one photo-bleaching retarder material includes at least one polymeric polyhydroxy compound selected from the group consisting of polyhydroxystyrenes, cellulose, and functionalized cellulose derivatives.

A high-intensity light source configuration has a long lifetime and can be modulated at a high rate. The configuration includes a movable member mounted to an actuator; a light-emitting phosphor region associated with the movable member; an input light source that illuminates the light-emitting phosphor region at a spot that is fixed relative to an emitted light output region; and a light source controller controlling the movable member actuator and the input light source. The input light source (e.g., laser) provides high-intensity input light to the illuminated spot, causing the light-emitting phosphor region to emit high-intensity output light. The light-emitting phosphor region is moved relative to the illuminated spot so as to reduce optical quenching and photobleaching, to thereby extend the life of the light source configuration. The phosphor region may emit broadband light and/or may include respective sub-regions having phosphors that emit respective peak wavelengths.

An optically readable media (10) has an information-encoding layer (16) and at least one color-forming layer (12) that embodies an optical readout-limiting mechanism. In a first embodiment the at least one color-forming layer contains an additive that does not interfere with the optical readability of the media for a duration of a readout period. The additive, upon exposure to a source of optical radiation that is suitable for reversing the color-forming layer from an optical readout inhibiting state to an optical readout enabling state, undergoes a transformation that maintains the color-forming layer in the optical readout inhibiting state. More specifically, exposure to the source causes the color-forming layer to photobleach and the additive to oxidize, where the oxidation of the additive permanently inhibits the optical readability of the media. The additive may be a leuco dye. In one embodiment the color-forming layer is comprised of 3-[2,2-bis(4-diethylaminophenyl)vinyl]-6-dimethylaminophthalide and the additive is comprised of benzoyl leucomethylene blue. In a further embodiment the additive is placed into a protective layer (18) that overlies the color-forming layer.

A system and method employing photokinetic techniques in cell biology imaging applications are disclosed. Systems and methods of acquiring image data of an object may comprise: selectively inducing photoactivation of material at a site on the object; performing an optical axis integration scan; simultaneously executing a time delay integration scan sequence; and processing acquired image data in accordance with one or more desired analyses. Various methodologies and applications may include, inter alia, selective photobleaching of a site on the object, diffusion rate, velocity, and wave-front propagation analyses, multi-dimensional analyses of dispersion characteristics, biomolecular binding in cellular organelles, and photoactivation assisted systematic image segmentation for the study of cellular components.

A high-intensity light source configuration has a long lifetime and can be modulated at a high rate. The configuration includes a movable member mounted to an actuator; a light-emitting phosphor region associated with the movable member; an input light source that illuminates the light-emitting phosphor region at a spot that is fixed relative to an emitted light output region; and a light source controller controlling the movable member actuator and the input light source. The input light source (e.g., laser) provides high-intensity input light to the illuminated spot, causing the light-emitting phosphor region to emit high-intensity output light. The light-emitting phosphor region is moved relative to the illuminated spot so as to reduce optical quenching and photobleaching, to thereby extend the life of the light source configuration. The phosphor region may emit broadband light and/or may include respective sub-regions having phosphors that emit respective peak wavelengths.

An instrument is disclosed for fluorescence assays which is capable of reading many independent samples at the same time. This instrument provides enhanced throughput relative to single-sample instruments, and is well-suited to use in general fluorescence, time-resolved fluorescence, multi-band fluorescence, fluorescence resonance energy transfer (FRET), and fluorescence polarization. This invention is beneficial in applications such as high-throughput drug screening, and automated clinical testing. Also disclosed are means and methods for a fluorescence polarization measurement which is highly sensitive, inherently self-calibrated, and unaffected by lamp flicker or photobleaching. This fluorescence polarization invention can be practiced on a variety of fluorescence instruments, including prior-art equipment such as microscopes and multi-well plate readers.

An instrument to acquire and methods to obtain three-dimensional (3D) images from a series of two-dimensional (2D) images which are obtained without moving the relative positions of the target, the detector, or the focusing lens is disclosed. The 2D images consist of one centered image obtained with the aperture at the center of optical system, and at least two directional images obtained with apertures at off-axis locations. The images can be obtained simultaneously or sequentially. The blurred 2D images are sectioned by computational method using point spread function of the optical system resulting in a set of decoupled 2D layers of the 3D object. The layered images are then sharpened by deconvolution using point spread function. The 3D reconstructed image is displayed. This technique provides fast data acquisition and fast image reconstruction and eliminates problems associated with motion, phototoxicity and photobleaching.

The invention provides a fluorescent probe represented by the formula (I), wherein R1 and R2 are independently selected from H or an aromatic vinyl group, and at least one of R1 and R2 is the aromatic vinyl group; R3 is selected from H, F, Cl, Br, OH, OCH3, N(CH3)2 or C1-C6 alkyl; and R4 is selected from C1-C6 alkyl. Compared with the prior art, because the fluorescent probe provided by the invention has a relatively large electronic conjugated system and plane, the intensity of the intramolecular charge transfer effect can affect the molecular fluorescence emission intensity; when the fluorescent probe and the G-quadruplex generate a specific action, the flexibility of intramolecular rotatable double bonds is restricted, the intramolecular charge transfer effect is enhanced, and the fluorescence is also enhanced significantly; moreover, the fluorescent probe provided by the invention has the advantages of low biotoxicity, phototoxicity and photobleaching property, good light stability, good water solubility and good cell membrane permeability.

The invention discloses a preparation method and application of carbon dot/gold composite nanoparticles. Citric acid and cysteine are taken as raw materials, and N-S codoping carbon dots are synthesized; the carbon dots serve as the heterogeneous nucleation center of the gold nanoparticles, a reducing agent and a stabilizing agent, any other auxiliaries such as a surface active agent or an adsorbent do not need to be added, and the carbon dot/gold composite nanoparticles are prepared by further reducing chloroauric acid. The preparation method of the carbon dot/gold composite nanoparticles is simple, convenient, feasible, mild in reaction condition and environmentally friendly, and the prepared carbon dot/gold composite nanoparticles have the characteristics of being high in quantum yield, small in size, low in biotoxicity, resistant to photobleaching and the like, and can be applied to the fields such as cell imaging or catalytic reaction research.

The invention relates to a preparation method and a use of a fluorescent carbon quantum dot/polyacrylonitrile nanofiber membrane. The preparation method comprises that polyacrylonitrile and a fluorescent carbon quantum dot as raw materials are prepared into the fluorescent carbon quantum dot/polyacrylonitrile nanofiber membrane by an electrostatic spinning technology. The fluorescent carbon quantum dot/polyacrylonitrile nanofiber membrane is a nanofiber membrane material loaded with the fluorescent carbon quantum dot. The fluorescent carbon quantum dot/polyacrylonitrile nanofiber membrane can be used for selective fluorescence fast detection of Fe<3+> in water, has good heat stability and strong photobleaching resistance, and is an ideal sensitive material for preparation of a high-sensitivity thin film-type sensing device.

The invention discloses a rapid three-dimensional super-resolution microscopic method. The method comprises that a laser beam is converted into linearly polarized light after being collimated, phase modulation is carried out on the linearly polarized light, the linearly polarized light is converted into circularly polarized light, the circularly polarized light is projected to a sample to be measured, and pilot light emitted by scanning points of the sample to be measured is collected; and 3D scanning is carried out on the sample. Phase modulation comprises primary phase modulation and secondary phase modulation, a spatial light modulator which modulates the phase of an s light component of the linearly polarized light is used in the primary phase modulation, a spatial light modulator which modulates the phase of a p light component of the linearly polarized light is used in the secondary phase modulation, and a 3D super-resolution image is obtained according to the effective pilot light intensity. The invention also discloses a rapid 3D super-resolution microscopic device. According to the invention, optical power is lower, the photobleaching effect is weakened, 3D imaging is rapid, the device is simple, and light splitting is not needed.

A system and method of providing illumination for a cell imaging system delivering modulated light for illumination of a sample. Modulation of illumination intensity may be effectuated by pulsing the output of a light source, for example, or by selectively interrupting light provided by the source. Such modulated illumination may have particular utility in applications where reducing the number of electrons in a super-excited state may minimize the rate of photobleaching and photodamage in the molecules being studied.

The present invention provides a method for fabricating an electroluminescent EL display, wherein the individual color pixels are formed by doping a common blue-emitting host with two or more photo-bleachable (or photo-oxidizable) dopants, such as red and green emitting organic materials. The host may also be doped with a blue emitting material that is not photo-bleachable.

The invention discloses a green synthetic method for blue fluorescence carbon quantum dots with high fluorescence quantum yield, and belongs to the technical field of fine chemical engineering. The method adopts a mixture of citric acid and glycine as a raw material, the solid mixture is heated directly to a certain temperature for a certain time, so that the solid mixture is dehydrated, after cooling to indoor temperature, the mixture is extracted by a low boiling point organic solvent, and the fluorescence quantum yield of the obtained carbon quantum dots is much higher than the fluorescence quantum yield of carbon quantum dots which are not processed by the organic solvent. The adopted raw material is easy to obtain, cheap and free of poison, three wastes are not generated in the preparing process, the operation is simple and convenient, and the raw material is easy to control. Due to the fact that the higher high fluorescence quantum yield, blue fluorescence emission and good photobleaching resistance are achieved, the carbon quantum dots can serve as a fluorescent whitening agent and be applied to production of paper pulp, scouring agents and washing powder, and the carbon quantum dots can serve as fluorescence markers of biomolecules.

The present invention includes a microscope and a method for using the microscope for single molecule with reduced photobleaching of a fluorophore (20) that includes a light translucent material (16); a metal layer (18) disposed on the light translucent material (16); a medium (15) disposed on the metal layer (18), the medium (15) having one or more fluorophores (20) capable of binding a target analyte (e.g., inside a cell); a microscope positioned to observe the surface plasmon emissions from the one or more fluorophores (20) within 50 nanometers of the surface of the metal layer (18); an excitation source capable of exciting the one or more fluorophores (20), the excitation source positioned to strike the light translucent material (16) at a first angle; and a light detector (38) that selectively detects emitted light generated by excited fluorophores (20) at a second angle (22), wherein light emitted by the one or more fluorophores (20) at the surface plasmon angle is detected through the microscope, such that single molecules may be detected without significantly degrading fluorophore (20) emissions.

The invention belongs to the field of electroluminescent cells, and relates to a method for prolonging the service life of a quantum dot LED. The method is that the volume ratio of a shell to a kernel of a quantum dot used as a lighting layer is more than 60: 1; the shell and the kernel of the quantum dot selected are both manufactured from materials in the II-VI cluster, and the materials can be CdSe/ ZnS, CdSe/ ZnCdS/ ZnS, CdSe/ CdS, ZnCdSe/ ZnS or ZnCdSe/ ZnSe/ ZnS; the shell layer can be formed by compounding ZnCdS and ZnS, or ZnSe and ZnS. According to the method, the quantum dot with huge shell is used as the lighting layer, so that the fluorescent blinking of the quantum dot can be effectively inhibited, and the photobleaching resistance can be improved; the service life of the electroluminescent cell of which the lighting layer is a semiconductor fluorescent quantum dot can be effectively improved.

A pulse photobleaching methodology wherein a monochromatic illumination (e.g., laser illumination) having a higher power intensity (photobleaching power) just below the photodamage threshold of a luminescent sample is initially used to significantly attenuate sample luminescence without photothermally destroying the sample material. Thereafter, the laser power density may be reduced to a significantly lower level (analytical power level) to carry out spectroscopic measurements (e.g., collection of Raman scattered photons) on the sample. In one embodiment, the laser illumination wavelength remains the same despite changes in laser power intensity. Some figures-of-merit may be computed from optical measurements made at the analytical power level to guide the photobleaching process. Sample-dependent combinations of laser power density and short exposure times may be obtained to significantly expedite photobleaching to assist in collection of sample spectral data in the field without a long wait. Portable spectroscopy systems employing pulse photobleaching may be devised for expeditious collection of spectral data from luminescent samples in the field.

A method for delivering photodynamic therapy (PDT) while performing dose metric monitoring and treatment feedback-driven control is presented. Photodynamic therapy is initiated with irradiation of light at a first irradiance. A set of fluorescence and reflectance spectroscopic measurements are taken at prescribed intervals during the therapy of the treatment region. Spectra are analyzed to determine dose metrics of the therapy such as fluorescence photobleaching of the sensitizer and blood oxygen status and optical properties of the treatment region. This information is then used to determine an optimal fluence rate given those parameters and the region is irradiated with a second irradiance. This process is continued until either the entire prescribed fluence is delivered to the region or a predetermined extent of photosensitizer bleaching is achieved.