33 results about "Phototoxicity" patented technology

The invention specifically discloses a beta-diketone iridium complex as well as a preparation method and application thereof, and relates to the technical field of biological medicines. The beta-diketone iridium complex provided by the invention has high phototoxicity and low dark toxicity, can be applied to tumor cells as effective PSs of PDT, and is specifically delivered to the tumor cells by taking a traditional Chinese medicine monomer tripterine as a supplement in combination with a nano-targeting delivery technology for photodynamic / immune synergistic treatment of hypoxic tumors.

The application discloses a compound morinda officinalis color-increasing liniment for treating vitiligo and a preparation method thereof, and particularly relates to the technical field of traditional Chinese medicine preparations, wherein the liniment is prepared from morinda officinalis, spilanthes acmella, giant knotweed, caesalpinia sappan, psoralea corylifolia, radix angelicae dahuricae and rhizoma smilacis glabrae as raw medicinal materials. The preparation method comprises the following steps: dynamic reflux extraction, percolation extraction, macroporous adsorption resin purification and preparation molding. Through the classification extraction and purification process, the photosensitive active ingredients are retained, and the photo-toxic impurities are effectively removed, so that the photo-toxicity is completely negative. The pharmacodynamic test shows that the liniment has a significant curative effect on vitiligo. The safety test shows that the liniment has no skin irritation and no allergenicity, and has excellent light safety. The quality research shows that the quality of the liniment is stable and controllable. The liniment has definite curative effect, high safety and stable quality, and has a good clinical application prospect.

The application belongs to the technical field of molecular biology, and relates to a polypeptide with a light protection function and application of the polypeptide. An amino acid sequence of the polypeptide comprises a sequence shown in SEQ ID No. 1 or SEQ ID No. 2. The polypeptide disclosed by the application is derived from heat-soluble protein, has a significant light protection effect, can effectively prevent and repair skin damage caused by light, has strong stability, has no sensitization, has no phototoxicity, and has a good application prospect.

The present application provides a kind of fluorine boron compound, the fluorine boron compound contains anthraquinone group, can generate strong oxidizing anion radical after photoexcitation efficiently, thus has lower half inhibitory concentration, stronger inhibitory effect, has stronger phototoxicity, can kill cancer cells in shorter time;Meanwhile, the superoxide anion radical generated can specifically oxidize FADH2 coenzyme in mitochondrial electron transport chain, by oxidizing its isoalloxazine ring structure to cause coenzyme inactivation, then mitochondrial electron transport process, and inhibit cancer cell energy metabolism, so as to effectively inhibit the proliferation activity of cancer cells.

The invention discloses a self-assembled fluorescent probe as well as a preparation method and application thereof. The preparation method comprises the following steps: adding 1-ethyl-2-methylbenzo [cd] indole-1-onium into a mixed solution of 10-[4-(dimethylamino) phenyl]-7-phenyl-3, 11-dithia-7-aza-tricyclo [6.3. 0.02, 6] undec-1 (8), 2 (6), 4, 9-tetraene-4-formaldehyde, acetic acid, acetic anhydride and triethylamine, and then mixing with methyl tert-butyl ether, so as to obtain the small molecule DMA-IN3, and carrying out ultrasonic self-assembly to obtain the nano-particle DINP. The self-assembled fluorescent probe DINP obtained by the invention has fluorescence property and phototoxicity, can be enriched to tumor tissues in a targeted manner, generates a large amount of heat under an illumination condition, realizes visual diagnosis and treatment of tumors, is good in biological safety, and provides a new scheme for clinical tumor treatment.

The invention relates to a preparation method and application of a novel RNA-targeted selenium-containing photosensitizer, and belongs to the technical field of medical chemistry and biological medicine. The invention designs and synthesizes a photosensitizer RNA-S for targeting RNA (Ribonucleic Acid), and the RNA-S is a novel multifunctional near-infrared photosensitizer constructed on the basis of a 2, 1, 3-benzoselenadiazole skeleton. The molecule shows high phototoxicity, low dark toxicity and good biocompatibility in tumor cells, and can effectively inhibit tumor cell growth and realize synchronous imaging. The invention provides a theoretical basis and a practical basis for developing a novel efficient selenium-containing photosensitizer, and has a very good clinical application prospect.

The application discloses a curcuminoid xanthene compound shown in formula I, a preparation method of the curcuminoid xanthene compound and application of the curcuminoid xanthene compound as a photodiagnosis and treatment agent. The compound has the characteristics of long absorption and emission wavelength, good stability, high phototoxicity, large Stokes shift, good biocompatibility and good cell membrane permeability. Compared with curcumin, the photosensitizer has a longer absorption wavelength, lower dark toxicity and can generate ROS under type-I and type-II synergistic action, and is expected to be applied to PDT in an anaerobic tumor microenvironment.

The invention relates to the technical field of biomedical materials, in particular to a nano assembly for releasing NO through red light triggering and a preparation method and application of the nano assembly. The nano assembly is prepared from an amphiphilic block polymer with a polyethylene glycol hydrophilic end and a hydrophobic segment containing NO donor molecules and photocatalyst molecules through a hydrophilic and hydrophobic self-assembly forming method. Photocatalyst molecules in the amphiphilic block polymer are excited under irradiation of red light, decomposition of NO donor molecules can be triggered through an electron transfer process, and space-time controllable adjustment of NO release is achieved. The nano assembly has the red light response characteristic, excellent biological safety and antibacterial property, and has a wide application prospect in the aspects of antibiosis, anti-inflammation and the like. In addition, the red light has the characteristics of large tissue penetration depth and low phototoxicity, so that the problem that the penetration depth of the traditional ultraviolet light or blue light response NO donor molecular monomer is limited is effectively solved.

This invention discloses a method for preparing tree tomato leaf extract, its products, and applications, belonging to the field of natural product extraction and application. The method involves taking tree tomato leaf powder, using a 60% ethanol aqueous solution as the extraction solvent, extracting at a material-to-liquid ratio of 1:30 g / mL and a temperature of 60°C for 45 min, repeating the extraction twice, combining the two filtrates, concentrating, and drying to obtain the tree tomato leaf extract. This extract is highly enriched with polyphenols, flavonoids, and other active ingredients, exhibiting multiple effects such as antioxidant, anti-inflammatory, tyrosinase inhibition, and promotion of hyaluronic acid synthesis. It has also been confirmed that it has no significant cytotoxicity, phototoxicity, or skin sensitization. Cosmetic compositions containing this extract can effectively improve skin hydration, barrier function, redness, and dullness, and are suitable for preparing multifunctional pharmaceuticals or cosmetics with anti-inflammatory, whitening, moisturizing, and anti-aging properties, providing strong support for the high-value utilization and development of tree tomato leaf resources.

This invention belongs to the field of pharmaceutical chemistry technology, specifically relating to a near-infrared II luminescent iridium(III) complex photosensitizer, its preparation method, and its application. The near-infrared II luminescent iridium(III) complex photosensitizer disclosed in this invention generates singlet oxygen (…) under 550 nm illumination. 1 O2), hydroxyl radicals (•OH), superoxide anions (O2) •‑ We evaluated the antitumor activity of near-infrared luminescent iridium complexes using mouse breast cancer cells (4T1). Under unilluminated conditions, the near-infrared II luminescent iridium(III) complex photosensitizers showed no significant cytotoxicity to 4T1 cells, while under illumination, they exhibited good phototoxicity to 4T1 cells, with an IC50 value of [missing value]. 50 The concentration is 15.92 μM. We have invented a near-infrared II luminescent iridium(III) complex photosensitizer, which has excellent photoimmunodynamic therapeutic effects and will have important application prospects in the treatment of hypoxic tumors.

Intraluminal photodynamic therapy (PDT) of a target tumor tissue that is carried out from within a blood vessel is disclosed. The PDT method may be a vascular-targeted photodynamic therapy (VTP) or any other form of PDT where photosensitizer concentration in the blood vessel endothelium is not phototoxic. The PDT of this disclosure is carried out from within a blood vessel in the vicinity of the target tissue or organ with a light-diffusing section of an irradiating optical fiber being positioned within the confines of an inflatable balloon section of a balloon catheter, which is inflated during irradiation to occlude blood flow.

This invention belongs to the fields of biomedicine and fluorescent probe technology, and relates to a biheaded functional molecule and its application in the preparation of products for imaging cell tailpods or migration bodies. The biheaded functional molecule has the chemical structure shown in Formula I; wherein R is a hydrophilic positive ionic fluorescent group, X is methylene or O, Y is methylene or carbonyl, and n is 1~3. The biheaded functional molecule provided by this invention can achieve long-term cell membrane anchoring and can dynamically track the migration body formation process with low phototoxicity, low background fluorescence, high photostability, and high brightness.

This paper describes a class of dyes containing 1,4-diazabicyclo[2.2.2]octane, their preparation methods, and applications, belonging to the fields of optical diagnostics and biomedicine. The dye comprises the dye molecule and 1,4-diazabicyclo[2.2.2]octane covalently coupled to the dye. 1,4-diazabicyclo[2.2.2]octane directly quenches singlet oxygen generated by the dye, affecting the triplet state of the dye, thereby improving its anti-bleaching ability and photostability, and reducing phototoxicity during fluorescence imaging. Furthermore, the introduction of 1,4-diazabicyclo[2.2.2]octane significantly improves the brightness of the dye, inhibits photoscintillation, and enhances its water solubility. Its protonation characteristic enhances the dye's mitochondrial targeting ability. These compounds, by reducing singlet oxygen and enhancing the local photothermal and reactive oxygen species production in mitochondria, induce potent cell necrosis, apoptosis, and immunogenic cell death, comprehensively improving anticancer efficacy. These compounds exhibit excellent photostability and low skin toxicity, showing broad application prospects.

The invention discloses a preparation method of a phallus impudicus extract as well as a product and application of the phallus impudicus extract, and belongs to the technical field of biology. The phallus impudicus extract is prepared by taking phallus impudicus buds as a raw material and adopting a water extraction process, and the mass percentage of polysaccharide in the extract is not less than 70%. The obtained extract has the biological activity effects of resisting oxidation, resisting inflammation, resisting saccharification, inhibiting elastinase, relieving repair activity or promoting skin repair, and experiments prove that the extract is free of cytotoxicity, sensitization and phototoxicity. The phallus impudicus extract is very suitable for sensitive skin and used as a daily skin care product. Development of traditional fungus phallus impudicus is led to the field of high-tech cosmetic raw materials, a complete technical scheme and solid data support are provided for high value-added utilization of phallus impudicus, and the phallus impudicus is wide in application prospect.

This invention discloses a method for preparing anthocyanin extract from tree tomato, along with its products and applications, belonging to the field of natural plant extraction and application. The method uses tree tomato peel as raw material, employing an acidic ethanol aqueous solution containing 0.5% (w / v) trifluoroacetic acid as the extraction solvent, at a material-to-liquid ratio of 1:30 (g / mL), at a temperature of 50℃, and for 60 minutes to obtain a high-content anthocyanin extract. This tree tomato anthocyanin extract possesses excellent anti-inflammatory, antioxidant, anti-glycation, elastase-inhibiting, and whitening activities, and exhibits low cytotoxicity, no phototoxicity, and no skin sensitization. The efficient and stable anthocyanin extraction process provided by this invention has high extraction efficiency and good enrichment effect of active ingredients, meeting the requirements for subsequent industrial production. The obtained extract has whitening, anti-aging, and anti-inflammatory effects, providing a high-quality raw material and formulation solution for developing multifunctional and highly safe natural plant-based skincare products.

The invention provides a photosensitizer with high singlet oxygen quantum yield as well as a preparation method and application thereof, and belongs to the technical field of coordination chemistry and biomedicine. According to the photosensitizer with the high singlet oxygen quantum yield, two carbazole groups are specifically introduced into a phenanthroline ligand of a ruthenium complex for modification. According to the structural modification strategy, the light absorption capacity of the complex in a visible light region is remarkably enhanced, and the singlet oxygen quantum yield of the complex is improved in a breakthrough manner. In-vitro cell experiments show that the compound shows excellent phototoxicity to various tumor cell strains, has very low toxicity under dark conditions, and shows good photodynamic therapy selectivity. An in-vivo tumor-bearing mouse model experiment proves that tumor growth can be effectively inhibited under an illumination condition, and obvious systematic toxicity is not observed. The photosensitizer disclosed by the invention has the remarkable advantage of extremely high singlet oxygen quantum yield, and shows huge application potential and development value in the field of tumor photodynamic therapy.

This invention discloses a VH032-modified phthalocyanine, its preparation method, and its applications. A chemical synthesis method involving amino and carboxyl coupling is employed. The photosensitizer ZnPc and the Von Hippel-Lindau (VHL) ligand VH032 are linked using either no linker or an alkyl or PEG chain as a linker to construct the VH032-modified phthalocyanine. This drug can degrade VHL after light irradiation and, combined with photodynamic therapy (PDT), efficiently kill tumor cells, achieving a phototoxicity index (PI) of 36397. Utilizing the more active regulation of Cyclin Dependent Kinase 2 / 4 (CDK2 / 4) by VHL in bladder cancer tumor tissue, after light irradiation, this compound exhibits a selectivity index (SI) of 749 for killing bladder cancer cells compared to normal cells, achieving selective killing of tumor cells under light conditions and effectively avoiding the risk of damage to adjacent normal tissues during treatment.

The invention belongs to the technical field of biomedical engineering and drug delivery, and particularly relates to photoresponsive polydopamine-based composite nanoparticles as well as a preparation method and application thereof. Aiming at the problems of high system toxicity, poor controllability and the like of a photosensitizer in the existing photodynamic therapy, the photosensitizer is synergistically constructed by a temperature-sensitive upper critical solution temperature polymer carrier and a polydopamine core, the photosensitizer is loaded through a physical adsorption effect, and a targeted neovascularization function is realized by modifying RGD (arginine-glycine-aspartic acid), so that the final LCPN is formed. The mPDA inhibits the activity of the photosensitizer through a fluorescence quenching effect, and the non-targeted phototoxicity in the circulation process is remarkably reduced; the polymer (P (NADAm-co-NPhAm)) is subjected to temperature control phase change under the triggering of near-infrared light, so that the controllable release of PS is realized. PS released by the LCPN is excited by near-infrared light at a target part to perform photodynamic therapy so as to accurately block new vessels, and meanwhile, an mPDA kernel can remove residual active oxygen and relieve inflammation of surrounding tissues.

The invention relates to an ionic iridium (III) complex based on (+ 3, + 2, + 1)-[Ir (NCN / NNN) (CN) (R)] < + / 2 + > configuration and a preparation method and application thereof, and the preparation method comprises the following steps: 1, reacting a tridentate ligand NCN / NNN with iridium chloride trihydrate in a specific solvent to obtain a first intermediate; 2, reacting the obtained first intermediate with a bidentate ligand HCN to obtain a second intermediate; and step 3, substituting the second intermediate with a monodentate cyanide ligand R to obtain a target product. The complex is efficient in synthesis method, has good photophysical properties, and has low cytotoxicity and high phototoxicity index for lung cancer cells, colon cancer cells and liver cancer cells, which shows that the complex has good application prospects in the field of metal anti-cancer drugs.

The application belongs to the technical field of biological medicine, and particularly relates to a preparation method and application of a long-conjugated pyridinium macrocycle photosensitizer. In order to synthesize a novel pyridinium macrocycle photosensitizer molecule with high singlet oxygen yield, high stability and low toxicity for photodynamic therapy, the application provides a long-conjugated pyridinium macrocycle photosensitizer and a preparation method thereof. The long-conjugated pyridinium macrocycle photosensitizer is simple to prepare and has high yield. Meanwhile, the preparation method of the photosensitizer has the advantages of easy-to-obtain raw materials, good repeatability, high yield, high singlet oxygen generation efficiency, low dark toxicity, high light toxicity and good photodynamic application prospect.

The invention provides a crystal form of an N-(4-methylphenyl)-2-pyridone compound as well as a preparation method and application thereof, and particularly relates to application of the N-(4-methylphenyl)-2-pyridone compound in treatment of fibrosis, pneumoconiosis and fatty liver. The N-(4-methylphenyl)-2-pyridone compound crystal provided by the invention is relatively good in solubility, simple in preparation operation and good in repeatability, industrial production can be realized, the cost is reduced, and the production period is shortened. In addition, the crystal has lower phototoxicity, is obviously superior to a drug pirfenidone on the market, has excellent effects on treating fibrosis, pneumoconiosis and fatty liver, and has extremely high clinical application capability.

This invention discloses an activated water-soluble photodynamic antibacterial agent based on bacterial bioorthogonal labeling and its application. The activated water-soluble photodynamic antibacterial agent of this invention contains hydrophilic groups, which can prevent uptake by normal cells, thereby reducing phototoxicity to normal tissues. In addition, the activated water-soluble photodynamic antibacterial agent of this invention is modified with bioorthogonal reactive groups that can quench the fluorescence and ROS generation capabilities of photosensitizers. This allows for rapid click chemistry reactions with active groups inserted into peptidoglycans during bacterial metabolism, achieving specific labeling and in-situ activation of the photosensitizer on the bacterial surface. Under near-infrared light irradiation, the photodynamic antibacterial agent specifically modified on the bacterial surface can generate a large amount of reactive oxygen species, selectively killing bacteria while avoiding damage to normal tissues, exhibiting broad-spectrum antibacterial activity and showing promising application prospects in photodynamic antibacterial therapy.

This invention belongs to the field of biomedical technology, specifically relating to a near-infrared oxanthracene dye with tunable structural symmetry, its preparation method, and its application. Its structural formula is shown in formula Ia or Ib. This dye exhibits high quantum yield (up to 79.5%), low dark toxicity and phototoxicity, or, under near-infrared laser irradiation, can effectively produce type I (… 1 O2) and type II (•OH, O2• ‑ Reactive oxygen species can enable precise visualization imaging of mitochondria and achieve highly effective treatment of tumor cells by inducing ferroptosis.

The application belongs to the field of liposome drug delivery, and relates to a hypoxia-responsive prodrug liposome and a preparation and application thereof. The liposome contains a hypoxia-responsive prodrug and a photosensitizer, wherein the mass ratio between the hypoxia-responsive prodrug and the photosensitizer is 10:1-1:10; the hypoxia-responsive prodrug is a poorly soluble antitumor drug modified by a polyphenol. The hypoxia-responsive liposome prepared by the application has uniform particle size, high encapsulation efficiency and high drug loading capacity, good stability, significantly prolonged drug in-vivo circulation time and tumor site accumulation, and significantly improved antitumor effect. At the same time, the co-loaded liposome maintains fluorescence quenching in blood circulation, and specifically restores active oxygen capacity under the action of high glutathione in the tumor site, solving the problem of traditional photosensitizer phototoxicity.

The application discloses a kind of mitochondrial DNA targeted 4-amino benzopyran photosensitive dye and its preparation method and application, wherein the structure general formula of 4-amino benzopyran photosensitive dye is as follows: The preparation method of the 4-amino benzopyran photosensitive dye and its application in the preparation of mitochondrial DNA photosensitive imaging agent and mitochondrial DNA targeted tumor photodynamic therapy photosensitizer are also specifically disclosed.The photosensitive dye has excellent photosensitive activity and spectral feedback performance, which improves the in vivo tumor treatment efficacy.The 4-amino benzopyran photosensitive dye prepared by the application has low biological toxicity and high phototoxicity in in vivo tumor treatment, and the raw material is easy to obtain, the structure is simple, easy to prepare and easy to industrialize.

This invention discloses the application of glycyrrhizin in the preparation of cosmetics and its cosmetic compositions, belonging to the field of traditional Chinese medicine research and development. Glycyrrhizin is derived from licorice extract purified from licorice raw materials, with a purity of ≥98%. Based on its antioxidant, anti-inflammatory, anti-glycation, elastase inhibitory, and cell migration and repair promoting functions, glycyrrhizin can be used to prepare anti-aging or soothing repair cosmetics. Furthermore, within its effective concentration range, it exhibits no cytotoxicity, non-sensitization, or phototoxicity, making it suitable for preparing anti-aging or soothing repair cosmetic compositions. This cosmetic composition has been proven to significantly improve skin elasticity, reduce stinging and redness, and possesses anti-aging and skin-soothing repair effects, making it widely applicable for the daily maintenance and stabilization of sensitive skin. This invention fills a gap in the application of glycyrrhizin in the cosmetic field, providing favorable support for the widespread application of licorice in cosmetics.

The invention belongs to the technical field of molecular biology, and relates to a polypeptide with a light protection effect and application thereof, and the amino acid sequence of the polypeptide comprises a sequence shown as SEQ ID No.1 or SEQ ID No.2. The polypeptide is derived from hot-soluble protein, has a remarkable light protection effect, can effectively prevent and repair skin injury caused by illumination, is high in stability, free of sensitization and phototoxicity and has a good application prospect.

The invention relates to an application of second harmonic imaging in collagen detection. The application comprises the following steps: irradiating a sample containing collagen by utilizing near-infrared laser, collecting a second harmonic signal generated by the collagen in the sample, and carrying out imaging and quantitative analysis on collagenous fibers based on the second harmonic signal. A collagen detection scheme based on secondary harmonic imaging is developed, samples are not damaged, low photobleaching and phototoxicity are achieved, the resolution ratio is high, dynamic ultra-long space-time in-vivo imaging can be achieved, a scheme for detecting myocardial fibrosis is further developed, in-situ and in-vivo space-time dynamic imaging on myocardial fibrosis is achieved for the first time, and the method has the advantages of being high in sensitivity, high in sensitivity and the like. An important tool is provided for accurate detection of myocardial fibrosis, and the method can be effectively applied to construction of products for detecting myocardial fibrosis, screening / evaluation of therapeutic drugs and the like.

The application discloses a kind of karyolin-fluorine boron pyrrole photosensitizer and preparation and application in anti-breast cancer, comprising compound with structure as shown in general formula (I). Experimental results show that the photosensitizer has high light stability, high in-vivo and in-vitro singlet oxygen quantum yield;Cell results show that the photosensitizer has high photodynamic activity (10-40nM) and low dark toxicity (>100 μM) to different sources and different types of breast cancer cells, high selectivity index (selectivity index=dark toxicity IC 50 / phototoxicity IC 50 , range in 270-9400), with good treatment window;The photosensitizer also has lipid droplet and endoplasmic reticulum positioning function, can induce breast cancer cells to occur iron death;Mouse breast cancer model also confirms that the photosensitizer can effectively inhibit the tumor growth of breast cancer tumor-bearing mouse.

This invention discloses a near-infrared light-excited iridium complex, its preparation method, and its application in anti-breast cancer treatment, comprising the structure shown in formula (I): The complex of the present invention exhibits a strong inhibitory effect on the growth and proliferation of mouse breast cancer cells (IC50). 50 The concentration of the phototoxicity was 0.31 μM, while under dark conditions, its cytotoxicity was only 93.9 μM, and the phototherapy index (PI) was as high as 301. This is of great significance for the research of metal drugs for anti-tumor purposes such as breast cancer.