93 results about "Microscope imaging" patented technology

The invention relates to an edge enhancement imaging system. The system comprises a point light source; the object side polarization filtering unit is arranged on the light emitting side of the point light source and modulates the light into specific polarized light to irradiate a sample; the imaging unit is arranged on the light emitting side of the object side polarization filtering unit and is used for transmitting the input light field to an imaging surface; the spatial variable polarization modulation element is arranged on a conjugate surface of the point light source relative to the imaging unit and is used for performing spatial variable polarization modulation to obtain a modulated light field; and the image space polarization filtering unit is arranged between the space variable polarization modulation element and the imaging surface and is used for screening the modulated light field to obtain a light field in a specific polarization direction, and the light field in the specific polarization direction forms an edge enhanced imaging light field on the imaging surface. The invention also relates to a preparation method of the edge enhancement imaging system. The device has the advantages that the imaging effect of edge enhancement in any direction or all directions is achieved, a common microscope imaging system can be compatible, phase modulation is achieved through polarization state transformation, and sensitivity to wavelength is reduced.

The invention discloses a method for generating an enhanced image of a bright-field microscope, and the method comprises the steps: simulating an optical imaging model of optical Zernike phase contrast microscope imaging through a computer system based on the optical imaging principle of a conventional optical Zernike phase contrast microscope; extra hardware such as an annular diaphragm and an annular phase plate does not need to be added in an illumination light path and an imaging light path of the bright-field microscope, enhancement of the phase contrast of a bright-field digital microscopic image is achieved, energy loss of an illumination optical system is avoided, and the design and manufacturing cost of the microscopic optical system is greatly reduced; the optical Zernike phase contrast microscope can be universally used for different bright field microscopes, halo artifacts caused by interference effects in a traditional optical Zernike phase contrast microscope do not exist, finer structural features in biological cells can be distinguished, the resolution of the optical Zernike phase contrast microscope can be close to the resolution limit of objective lens design, and the technical requirements for users are greatly reduced.

SOLUTION: An apparatus includes a microscopy imaging module, a curved light sheet lighting module, and a sample scanning module. The curved light sheet lighting module generates a curvature-adjustable curved light sheet and operates in conjunction with a wide-field microscopy imaging apparatus having a field curvature, thereby enabling the entire imaging field to be maintained in focus. By integrating with the sample scanning module, high-throughput microscopy imaging of a centimeter-scale transparent sample is achieved without the need for field-of-view stitching.EFFECT: An apparatus and method are useful for solving the technical problems associated with existing light sheet microscopy technology, which suffers from low throughput and requires field-of-view stitching when imaging a centimeter-scale transparent sample. They also contribute to addressing a field curvature issue in microscope objective lens design, thereby enabling reduced design complexity and production cost for a high-throughput objective lens.SELECTED DRAWING: Figure 1

The invention provides an immunofluorescence detection method and system for dynamically detecting mouse uterus structure development based on multiple time points, and immunofluorescence staining comprises the steps of sheet unfolding, dewaxing, repairing, cell membrane punching, sealing, primary antibody treatment and secondary antibody treatment. The system comprises a sample pretreatment control module, a multi-channel confocal microscope imaging module and a data analysis module integrating fluorescence intensity calculation, Pearson co-localization analysis and a Western blot data integration algorithm. Through a multi-time-point and multi-index immunofluorescence detection method, dynamic and systematic data support is provided for mouse uterus development mechanism research, and an application basis is provided for fundamental research of reproductive biology and construction of related disease models.

The application provides a differential-based white light lensless single-frame weakly conjugate reconstruction method, which can realize weakly conjugate image reduction and improve single-frame imaging resolution in a white light lensless imaging system without using additional elements and complex algorithms. The method comprises the following steps: step one, obtaining spectral distribution information of a system light source by using a spectrometer and performing differential processing to obtain a reconstruction wavelength; step two, performing preprocessing on a collected single-frame image by using a hologram average value reduction method; step three, generating light intensity diagrams of different defocusing surfaces by using an angular spectrum propagation formula, and determining a defocusing distance range based on a definition discrimination factor; and step four, calculating differential distribution on different focal planes in the distance range obtained in step three based on numerical differentiation, and obtaining a final result by selection. The application can reduce the requirement of spectral width of a traditional lensless microscope, effectively reduce the cost of the system, improve the imaging resolution of the white light lensless microscope, and reduce the weakly conjugate image in the case of single-frame image collection.

Methods and systems for correcting intensity variations in a raw image acquired via a microscope imaging system are provided. In one example, a method comprises acquiring a reference image and developing an intensity model based on the reference image, wherein the intensity model comprises a predetermined number of a plurality of model parameters obtained by curve fitting of a single analytical function or a linear combination of a plurality of analytical functions; embedding model parameters of the intensity model of the reference image into the raw image for subsequent image processing; and correcting the raw image on demand based on the intensity model of the reference image.In another example, a method involves using the multitude of parameters of the intensity model as a hardware signature to indicate whether a multitude of parameters of the optical system is maintained.

To provide a technique of the above charged particle beam apparatus, a technique that can prevent the lowering of apparatus performance due to the influence of vibration and noise by an air blower (fan). The charged particle beam apparatus includes a body device that is provided in a body cover, a plurality of air blowers that are provided on the body cover, and a controller that controls the plurality of air blowers. The body device has a stage on which a sample is placed, and a microscope that images the sample. The controller obtains an image obtained by imaging the sample by the microscope, obtains vibration information from the image, compares the vibration information and a specification value, and on the basis of the result of the comparison, controls the numbers of rotations of the plurality of air blowers. The controller decreases the number of rotations of at least one first air blower among the plurality of air blowers, and increases the number of rotations of at least one different second air blower among the plurality of air blowers.

This invention discloses a method for correcting image distortion in a miniature microscope based on optical simulation and displacement field driving, comprising the following steps: establishing an optical model of the miniature microscope, performing field curvature analysis, and generating a grid distortion map; extracting distortion data from sampling points and converting coordinate values ​​to pixel units according to the pixel size of the CMOS sensor; constructing a continuous displacement field covering the entire image using an interpolation algorithm based on the discrete sampling point data; performing geometric correction on the distorted image based on the displacement field, specifically including: correcting the original distorted image to a geometrically correct image based on the displacement field, employing an inverse mapping strategy, for each pixel position in the corrected image, querying its corresponding position in the original distorted image, and resampling; and evaluating and visualizing the correction results. This invention improves the accuracy of image distortion correction and enhances the imaging quality of the miniature microscope.

The application provides an adaptive atomic force microscope (AFM) imaging method based on block compressed sensing, and comprises the following steps: step S1: a plurality of sub-blocks with the same size are divided on the surface of an AFM sample by using an overlapping block mode; step S2: each sub-block is pre-scanned, a row direction is taken as a fast scanning direction, a lower left corner is taken as a starting point of pre-scanning, and a pre-scanning sampling rate is calculated; step S3: a BP neural network is used to train a sample database, a relationship between a characteristic parameter and a required minimum sampling rate is obtained, and a suitable total sampling rate of each sub-block is adaptively obtained; step S4: a required number of sampling points are randomly selected on the surface of a current sub-block, and adaptive scanning is performed by using a continuous random scanning mode; and step S5: after all sampling points of a sub-block are collected, TVAL3 reconstruction algorithm is combined to perform reconstruction, and finally, all reconstructed sub-blocks are combined together; and the block effect is eliminated by using the overlapping block mode, so that each sample can obtain high and uniform imaging quality.

The present invention relates to a method for correcting a spherochromatic aberration that occurs when examining a sample with a microscope. The microscope comprises an objective lens and a corrective device arranged in a beam path of the microscope for correcting a spherical aberration. According to the method, data for describing the spherical aberration as a function of a wavelength of light imaged by the microscope are provided. A sample is examined with the microscope, and at least one spectral range of the light imaged by the microscope is determined.A control parameter of the correction device is determined based on the provided data describing the spherical aberration as a function of wavelength for the at least one determined spectral range, in order to minimize the spherical aberration for the at least one determined spectral range. The correction device is then set to the determined control parameter. The invention further relates to a microscope, which is in particular a light microscope.

A method for generating an image (100) for a microscope, comprises receiving (102) a first image (210) and receiving (104) a second image (220). The method further comprises generating (106) a processed second image (240, 214) based on the second image (220) and generating the image (204) using the first image (210) and the processed second image (240, 214).

The invention relates to the technical field of microscopes, in particular to a light processing device, a fluorescent wide-field microscope, an imaging method, a medium and equipment, the light processing device comprises a first polarization beam splitter used for splitting incident light into first polarized light and second polarized light, the polarization directions of the first polarized light and the second polarized light are perpendicular to each other, the first polarized light enters a first light path, and the second polarized light enters a second light path; the second polarized light enters a second light path different from the first light path; the grating is positioned on the first light path, so that the first polarized light is modulated into structured light after passing through the grating; the second polarization beam splitter is used for combining the first polarized light modulated by the grating and the second polarized light not modulated by the grating to an emergent light path; and the polarized light selection assembly is positioned on the emergent light path and is used for selectively allowing the first polarized light or the second polarized light to pass through.

The application provides a kind of primary neuron cell golgi staining method and application based on lipid, comprising the following steps: 1) extracting, culturing and fixing primary neuron cell;2) selecting one kind of lipid in phosphatidylcholine, phosphatidylethanolamine, sphingomyelin, cholesterol, lecithin;3) the selected lipid is evenly covered on the cell surface, and Golgi staining solution is added for staining;4) remove Golgi staining solution, and use reducing agent for developing reaction;5) optical microscope imaging observation and / or simultaneous radiation X-ray imaging observation;6) the imaging data is analyzed and processed using data processing software.The application successfully stains primary neuron cell, and under the simultaneous radiation X-ray imaging technology, neuron dendrite and morphology can be observed, which lays a good research foundation for using simultaneous radiation X-ray to quantitatively study neuron complete three-dimensional structure and complete three-dimensional fine structure of neuron.

The invention relates to an automatic focus following method for a microscope, relates to the technical field of microscope imaging, and aims to construct a defocus distance prediction model by taking focusing as a regression problem so as to directly predict a defocus distance based on a picture. In the initial stage of focusing search, a gradient estimation algorithm without global scanning is provided based on a defocus distance prediction model, and the optimal focal plane is quickly converged through the adjustment steps of detecting a current area, predicting a focusing position, jumping to the focusing position and using the least imaging times and the least camera height, so that the focusing time is remarkably shortened, and the focusing efficiency is improved. The technical problems that an existing focusing algorithm is large in operand, and focusing to a wrong position is prone to occurring are solved.

The utility model discloses an optical microscope imaging module, and belongs to the technical field of bio-photonics. The module comprises a bright field imaging module and a bright field module shell or a fluorescence imaging module and a fluorescence imaging module shell. The problem that an existing traditional optical microscope is poor in adaptability and fixed in structure in diversified experiment requirements is solved. According to the optical microscope imaging module, the flexibility and adaptability of a microscope system are greatly improved. Through modular arrangement, scientific researchers can quickly replace or carry various functional modules according to different experiment requirements, so that the microscope can more accurately meet the requirements of specific experiments.

Disclosed in the present application are a method and apparatus for tracking an intra-operative OCT imaging position, a surgical microscope system, and a storage medium. The method for tracking an intra-operative OCT imaging position comprises: according to a historical position of a surgical instrument in a first coordinate system at a first moment, determining a predicted position of the surgical instrument in a second coordinate system at a second moment, wherein the first coordinate system is used for OCT imaging, and the second coordinate system is used for microscope imaging; according to the predicted position, determining a detected position of the surgical instrument in the second coordinate system at the second moment; and according to the detected position, determining a mapped position of the surgical instrument in the first coordinate system at the second moment, so as to track the intra-operative OCT imaging position.

The invention discloses an automatic algae identification method and system, and relates to the technical field of water environment monitoring. In the system, a multi-modal data acquisition module synchronously acquires a morphological image, characteristic spectrum data, characteristic fluorescence intensity and environmental parameter data of an algae sample through a microscope imaging unit, a spectrum detection unit, a fluorescence detection unit and an environmental parameter acquisition unit; the data preprocessing module is used for denoising, enhancing and normalizing the acquired multi-modal data; the feature extraction and fusion module extracts each modal feature through a preset network model, and performs weighted fusion by using an attention fusion algorithm; the classification and recognition module outputs an algae species recognition result based on the fusion features; and the cooperative control module coordinates the work of each module through a time sequence control signal to ensure the synchronism of data acquisition and processing.

The application provides an atomic force microscope imaging noise real-time control method and system, comprising: zero scanning noise template establishment, processing zero scanning data formed by zero scanning imaging of an atomic force microscope to obtain all noise frequency bands of the zero scanning data to constitute a noise deduction template; wherein, during the zero scanning imaging, all motion dimensions of the atomic force microscope used for the zero scanning imaging remain static; real-time scanning imaging noise suppression, using the noise deduction template for noise deduction during real-time scanning imaging of the atomic force microscope. The technical scheme of the application can obtain a noise deduction template according to the current environment of the atomic force microscope, and can cope with the characteristics of complex noise and vibration sources, rich frequency spectrum and strong time variation.

1. Name of the product in this design: In-vivo reflective confocal microscope imaging head (RCT-9503). 2. Purpose of this design: In medical institutions, it is used to collect, store, retrieve, display, and convert images of unstained epithelial cells and tissues on the body surface to assist doctors in clinical diagnosis. 3. The key design features of this product are the combination of shape and pattern. 4. The image or photograph that best illustrates the design's key points: a 3D model.

SOLUTION: An apparatus includes a microscopy imaging module, a curved light sheet lighting module, and a sample scanning module. The curved light sheet lighting module generates a curvature-adjustable curved light sheet and operates in conjunction with a wide-field microscopy imaging apparatus having a field curvature, thereby enabling the entire imaging field to be maintained in focus. By integrating with the sample scanning module, high-throughput microscopy imaging of a centimeter-scale transparent sample is achieved without the need for field-of-view stitching.EFFECT: An apparatus and method are useful for solving the technical problems associated with existing light sheet microscopy technology, which suffers from low throughput and requires field-of-view stitching when imaging a centimeter-scale transparent sample. They also contribute to addressing a field curvature issue in microscope objective lens design, thereby enabling reduced design complexity and production cost for a high-throughput objective lens.SELECTED DRAWING: Figure 1

The invention discloses a self-heuristic learning blind denoising method and system based on consistency difference guidance, belongs to the technical field of computer imaging, and solves the problems that the blind denoising performance of an SN2N method is insufficient under the condition of mixed noise distribution, the generalization ability of noise distribution is limited, and denoising of data with an extremely low signal-to-noise ratio is insufficient. The method comprises the steps that original picture data are collected and preprocessed, and an image data set is generated; generating a noise data pair by adopting an SN2N self-supervision method; performing consistency difference evaluation on the noise data pairs, and calculating a pixel-level difference chart; establishing a U-Net network based on a residual attention mechanism, wherein the network comprises an up-sampling module, a down-sampling module and a bottleneck layer; training the network by using a pixel-level difference graph, and constraining a loss function of the noise estimation branch according to the pixel-level difference graph; and adopting the trained network to predict and obtain a de-noising result. The method is suitable for living cell super-resolution microscope imaging and three-dimensional volume data denoising scenes.

The application discloses a kind of blood vessel microfluidic device and method suitable for glycolipid drug test, the device constructs three-dimensional biomimetic microvessel chip using induced pluripotent stem cells, and integrates multiple sensors;Through integrated environmental control module, programmed blood flow loading and drug exposure are realized.Intelligent analysis system includes multimodal intelligent observation module, based on microscope imaging and artificial intelligence speed measurement technology, three-dimensional flow field is inverted;Microcirculation digital twin module reconstructs blood vessels based on medical imaging and constructs calibratable hemodynamic model, for simulation and prediction;And wet-dry interaction module, through two-way interface, realizes "experiment-simulation" closed loop, injects entity data into digital twin to calibrate, and converts the prediction instruction of digital twin into chip control command to drive verification experiment.The system realizes complete technical chain from biomimetic model construction, intelligent observation, digital twin simulation to active experiment optimization.

The application discloses a kind of living cornea confocal tomography microscope imaging systems, belong to ophthalmic imaging technical field.The objective of the present application includes successively: first mirror group, diaphragm, second mirror group and third mirror group from image side to object side, objective surface covers corneal cap;Mirror group meets the proportion relationship of design between each other.The micro objective lens proposed in the present application can perform high-resolution imaging on corneal cells of different layers, and the imaging quality is close to the diffraction limit, and the experimental results prove that, cooperating with corneal cap, the present application can clearly obtain high-resolution images of cells in different layers within the range of 0-572 μm, and the object field is large, so it is not necessary to repeatedly move the inspection equipment to adjust the shooting area during the inspection process;Working distance is long, compared with prior art, doctors can obtain more operating space, significantly reduce the operation difficulty of corneal imaging, and further improve the diagnosis efficiency.

1. The name of the design product: in-vivo reflection type confocal microscope imaging head (RCT-9505). 2. The use of the design product: in medical institutions for collecting, storing, retrieving, displaying and converting images of body surface unstained epithelial cells and tissues, assisting doctors in clinical diagnosis. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best indicates the design points: perspective view.

The invention relates to the technical field of microscopic detection, in particular to a microscopic imaging system with bright field imaging and phase difference imaging, which comprises an imaging module, a bright field imaging illumination module, a focusing module, a phase difference imaging illumination light source module and a control module. The problems that in the prior art, the switching time is long, the definition degree of images is prone to being affected in the switching process, the target form of bright field image and phase difference image shooting is changed, and result contrastive analysis is not facilitated are solved.

The invention discloses a patterned wafer microstructure data acquisition system in a bright field environment and a data set construction method. The system comprises an adjustable light source assembly, a microscope imaging assembly and a three-axis electric displacement platform, the data set construction comprises the following steps: fixing a patterned wafer sample on a three-axis electric displacement platform, and setting illumination brightness through an adjustable light source assembly to obtain uniform bright field illumination; the three-axis electric displacement platform is controlled to move along a preset path, automatic focusing scanning in the Z-axis direction is executed at each point position, and an optimal focal plane image is generated; the collected images are classified after being cut and preprocessed, and normal samples and abnormal samples are distinguished; for the abnormal sample, generating a defect mask file through interactive labeling; and integrating images and masks to form a structured data set which can be directly used for algorithm research. According to the method, microscopic images with high depth of field and high contrast can be obtained, and a high-quality anomaly detection data set directly used for deep learning model training is constructed.

The application relates to an edge enhancement imaging system, which comprises a point light source, an object-side polarization filter unit arranged on the light-emitting side of the point light source and used for modulating light into specific polarized light to irradiate on a sample, an imaging unit arranged on the light-emitting side of the object-side polarization filter unit and used for propagating an input light field to an imaging surface, a spatial variable polarization modulation element arranged on the conjugate surface of the point light source relative to the imaging unit and used for spatial variable polarization modulation to obtain a modulated light field, an image-side polarization filter unit arranged between the spatial variable polarization modulation element and the imaging surface and used for screening the modulated light field to obtain a specific polarization direction light field, and the specific polarization direction light field forms an edge enhancement imaging light field on the imaging surface. The application also relates to a preparation method of the edge enhancement imaging system. The beneficial effect is that the imaging effect of arbitrary direction or full direction edge enhancement is realized, the common microscope imaging system can be compatible, the phase modulation is realized through polarization state transformation, and the sensitivity to wavelength is reduced.

The invention relates to the technical field of electron microscope imaging, in particular to an image imaging method and device, a storage medium and a computer program product. The image imaging method comprises the following steps: acquiring multiple frames of continuous high-angle annular dark field images of a target sample without irradiation damage; performing filtering processing and two-dimensional matrix conversion on each frame of high-angle annular dark field image to obtain a two-dimensional matrix data set; based on each two-dimensional matrix in the two-dimensional matrix data set, determining a first pixel point meeting a preset condition in each two-dimensional matrix; obtaining a first preprocessed image corresponding to each two-dimensional matrix based on the first pixel point corresponding to each two-dimensional matrix; on the basis of the first preprocessed image corresponding to each two-dimensional matrix, multiple frames of aligned second preprocessed images are obtained; and obtaining a target high-angle annular dark field image based on the multiple frames of second pre-processed images. According to the invention, filtering, three-dimensional stack reconstruction, alignment processing and the like are carried out on the high-angle annular dark field image, and the resolution and definition of imaging are improved.

A system can be used for imaging volumetric samples using a camera array to capture images under different illumination patterns at different axial planes of focus, and at optionally varying lateral fields of view. The sequence of images taken under the different illumination patterns and optional varying lateral fields of view can be processed to generate an image representation of the sample at a focus plane of the sample. Multiple image representations at different focus planes are assembled to form a 3D volumetric representation of the sample.

This invention relates to the field of femtosecond laser technology and discloses a multifunctional ophthalmic femtosecond device. The device includes: a scanning imaging module for emitting a probe beam and performing OCT imaging; a femtosecond laser scanning module for receiving the probe beam and irradiating the target tissue with the probe beam; the femtosecond laser scanning module for generating a femtosecond laser and focusing the femtosecond laser onto the target tissue; a surgical microscope module for receiving the probe beam and irradiating the target tissue; and the surgical microscope module for performing microscopic imaging. This invention integrates optical coherence tomography (OCT) technology, adding more imaging information to the original visible light image, which can effectively improve realignment accuracy and avoid the problem of separation and misalignment of corneal lenticule layers.