The invention discloses a soft matter comprehensive measuring device based on novel hybrid optical tweezers, comprising a laser, a first planoconvex spotlight, a second planoconvex spotlight, a first reversible reflector, a first reflector, a liquid crystal spatial light modulator, a second reversible reflector, a third planoconvex spotlight, a fourth planoconvex spotlight, a first dichroic mirror, a microscope objective, a two-dimensional electric platform, a halogen lamp, a microscope imaging tube mirror, a second dichroic mirror, a position detector imaging lens, a position detector, a camera and a computer, wherein the device can effectively work in the modes of high precision single optical tweezers and holographic optical tweezers, and the computer is respectively connected with theliquid crystal spatial light modulator, the camera and a position sensor signal inception system through signal lines. According to the invention, the measurement of simultaneous capture operation and interaction of a plurality of particles in liquid phase can be realized, and the measurement of broadband rheology characteristics of a microfluid by using a single particle probe can be realized.