Ultra-high resolution optical microscope imaging method and device
An ultra-high-resolution, optical microscopy technology, applied in the field of ultra-high-resolution optical microscopy imaging methods and devices, can solve the problems of equipment technical difficulty and cost rise, and achieve technical conditions that are easy to achieve, high-resolution, The effect of good image contrast
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[0013] The present invention is based on a transmission far-field optical microscope, and introduces a narrow-band filter (such as a central wavelength of about 430nm and a bandwidth of 10nm) and a ring aperture before a condenser. The parallel illumination light emitted by the LED strong light source can obtain the incident light of short wavelength (such as about 430nm) through the narrow-band filter, which can effectively improve the resolution of the microscope system; the annular aperture is equivalent to the aperture filter, allowing the high-frequency details of the sample The components pass through the optical system to effectively suppress the low-frequency diffuse components, thereby achieving high-resolution microscopic imaging; and after the oblique incident light generated by the annular aperture illuminates the sample, it enters a light-shielding disc in the form of an inverted hollow cone beam , only let the part of the light with large numerical aperture pass t...
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