Super-resolution imaging method based on fluorescence resonance energy transfer

A fluorescence resonance energy and super-resolution imaging technology, applied in the field of super-resolution imaging, can solve the problems of complex imaging software and high cost
CN102830101AActive Publication Date: 2012-12-19SHANGHAI INST OF APPLIED PHYSICS - CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI INST OF APPLIED PHYSICS - CHINESE ACAD OF SCI
Publication Date
2012-12-19

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Abstract

The invention discloses a super-resolution imaging method based on fluorescence resonance energy transfer (FRET). The super-resolution imaging method comprises the following steps: 1) marking a sample to be detected with a fluorescent probe having high FRET efficiency, wherein the fluorescent probe having high FRET efficiency has a marked FRET molecule pair, the FRET molecule pair includes a first fluorophore (a donor) and a second fluorophore (a receptor), and the first fluorophore can exert FRET on the second fluorophore; and 2) carrying out laser scanning confocal microscope imaging at an excitation light threshold with an excitation light intensity capable of allowing the FRET molecule pair in the step 1) to generate FRET. A super resolution technology based on saturated FRET in the invention can realize super resolution imaging of a biological sample on an ordinary laser confocal microscope, and the method provided by the invention has high resolution.
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Description

technical field

[0001] The invention belongs to the technical field of super-resolution imaging, in particular to a super-resolution imaging method based on fluorescence resonance energy transfer (FRET, Fluorescence Resonance Energy Transfer). Background technique

[0002] Many important research achievements in modern life science rely on the progress of microscopic imaging technology. However, it has been believed for a long time that the limit resolution that optical microscopy can achieve is about 200 nanometers, which is half the wavelength of light. Until the early 1990s, with the emergence of a series of new technologies in the field of optics, the optical diffraction limit was broken and the resolution of optical microscopes was increased to tens of nanometers.

[0003] The basis of super-resolution imaging technology mainly relies on various nonlinear optical effects. In 1991, a research group at Cornell University realized the multiphoton fluorescence imaging tech...

Claims

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