51 results about "Cells movement" patented technology

A Microscope Imaging System (CMIS) with intelligent controls is disclosed that provides techniques for scanning, identifying, detecting and tracking microscopic changes in selected characteristics or features of various surfaces including, but not limited to, cells, spheres, and manufactured products subject to difficult-to-see imperfections. The practice of the present invention provides applications that include colloidal hard spheres experiments, biological cell detection for patch clamping, cell movement and tracking, as well as defect identification in products, such as semiconductor devices, where surface damage can be significant, but difficult to detect. The CMIS system is a machine vision system, which combines intelligent image processing with remote control capabilities and provides the ability to auto-focus on a microscope sample, automatically scan an image, and perform machine vision analysis on multiple samples simultaneously.

A method and system of constrained optimization with linear constraints to remove overlap among cells of an integrated circuit. A coarse placement using well known methods may provide an initial placement of cells. Overlapping cells are separated. Any cell moved to its initial placement may be fixed so as not to be moved during subsequent placements. A plurality of linear inequalities representing allowable placements of a plurality of cells of a layout is generated. An objective function measuring cell movement subject to the constraints of the plurality of inequalities is minimized. The objective function minimizes cell movement from the initial cell placement. In this novel manner, large and small cells may be automatically simultaneously placed, deriving speed and quality advantages over prior art methods.

The invention discloses a flow cytometry instrument based on a micro-fluidic technology and a linear array detector. The instrument includes, in a successively connected manner, a micro-fluidic chip, a linear array detector and a data processing apparatus, and includes a light source which is located right above the micro-fluidic chip. A linear main channel is formed inside the micro-fluidic chip, wherein one end of the main channel is provided with a sample inlet while the other end is provided with a sample outlet. The lower surface of the micro-fluidic chip is adhered to the upper surface of the linear array detector. An included angle theta is formed between the main channel and the linear array detector, 0 degree <= theta <= 90 degrees. The flow cytometry instrument mainly combines the cell movement in multiple micro-fluidic channels in the micro-fluidic chip and scanning imaging of a traditional linear array detector, so that compared with a flow cytometry instrument based on an area array detector and the micro-fluidic technology, the flow cytometry instrument can form a cell image in higher resolution ratio, and is more accurate and has higher speed in cell flowing counting.

According to the present invention, timing information, connection information and physical information are received, and at the weighting determination step, the degree to which a cell can move is weighted. Then, at the movement range determination step, the movement enabled range of the cell is determined, and whether or not a cell placement area is available is decided. When it is decided that a cell placement area is available, the processing advances either to the cell movement area extension step or to the cell placement area acquisition step. Thereafter, an automatic, optimal placement process is performed for the cell.

The present invention concerns a method of genetic modification of a TGB-3 wild type viral sequence for reducing or suppressing the possible deleterious effects of the agronomic properties of a transformed plant or plant cell by said TGB-3 viral sequence, comprising the following successive steps: submitting said sequence to point mutation(s) which allow the substitution of at least one amino-acid into a different amino-acid; selecting genetically modified TGB-3 wild type viral sequences having said point mutation(s) and which are not able to promote cell-to-cell movement of a mutant virus having a dysfunctional TGB-3 wild type viral sequence, when expressed in trans from a replicon; further selecting among said genetically modified TGB-3 viral sequences, the specifically genetically modified sequence which inhibits infection with a co-inoculated wild type virus when the mutant form was expressed from a replicon, and recovering said specifically genetically modified TGB-3 viral sequence. The invention further relates to genetically modified TGB-3 viral sequences suitable to induce gene silencing. In particular hairpin constructs based on such sequences proved highly efficient to induce a PTGS mechanism and degradation of the whole of RNA2 thereby. When plants are transformed accordingly the spread of the virus in the plant is significantly reduced or blocked.

A novel generation magnetic therapy far infrared penis negative pressure cylinder cup device is suitable for penis magnetic therapy, far-infrared treatment and gas liquid negative pressure massage treatment of male sexual disfunction; a negative pressure cylinder inner body and a negative pressure cylinder outer body are independent units and are fixed together through screw connection on an connecting portion between the inner and outer bodies; a cavity is formed between the negative pressure cylinder inner body and the negative pressure cylinder outer body; a permanence magnet ring and an far infrared annular lamp ring are fixed in the cavity; the permanence magnet ring is positioned on an outer ring, the far infrared annular lamp ring is positioned on an inner ring; a power line connecting port is connected to power source through a connecting line so as to provided power for the far infrared annular lamp ring, thereby generating far infrared rays. The beneficial effects are that, the permanent magnet ring and the far infrared annular lamp ring are added between the negative pressure cylinder inner body and the negative pressure cylinder outer body so as to further improve and enhance blood circulation in the penis, the device can improve germ cell dynamic force and expand blood vessels, accelerates stone cell movements, enlarges central sinusoid in penis cavernosum, thereby providing environment space for cell fission regeneration.

The invention relates to a multi-layer holographic reconstruction and focusing strategy-based construction method of spatial field distribution situations of a cell group, and belongs to the field ofdigital holographic imaging application. The method comprises: setting a starting distance and a termination distance of the reconstruction and step length of each layer, carrying out layer-by-layer reconstruction, and recording a reconstruction situation of each layer; carrying out grayscale counting for a reconstruction image of a certain layer, using lognormal distribution to fit curvature extremums of a curve, setting grayscale thresholds, and combining a morphological processing means to extract a target object region; establishing a cell focusing judgment function, and adding informationof depth, at which focusing is best, after tracking and matching of the same cell imaging centroid between adjacent layers to form spatial positions where cells are located; and respectively calculating focusing depth of each cell in a hologram and horizontal-plane centroid projection in a reconstruction image of a corresponding layer to construct spatial distribution of the cell group. The method lays an important foundation for tracking multi-cell movement trajectories by multi-image holographic reconstruction, and is generally suitable for observation and measurement of microscopic non-cell objects.

A process of causing a targeted integration of DNA of interest into a plant cell nuclear genome, comprising; i) providing plant cells with an amplification vector, or a precursor thereof, capable of autonomous replication in plant cells, said vector comprising; a) DNA sequence(s) encoding an origin of replication functional in plant cells, b) DNA sequence(s) necessary for site-specific and/or homologous recombination between the vector and a host nuclear DNA, and c) optionally, further DNA of interest; ii) optionally providing conditions that facilitate vector amplification and/or cell to cell movement and/or site-specific and/or homologous recombination, and iii) selecting cells having undergone recombination at a predetermined site in the plant nuclear DNA.

The invention relates to the technical field of skin anti-aging products, discloses a formula of a skin full-layer anti-aging component, and solves the problems of relatively high anti-aging treatmentcost and poor treatment effect generally existing in anti-aging treatment methods in the current market. The formula comprises the following raw materials in parts by weight: 10 parts of hyaluronic acid, 15 parts of botulinum toxin, 18 parts of a mesoderm anti-aging product and 21 parts of a chemical skin rejuvenating solution. The hyaluronic acid, the botulinum toxin, the mesoderm anti-aging product and the chemical skin rejuvenating solution are simultaneously injected into subcutaneous tissues in a combination ratio of 1: 1.5: 1.8: 2.1, and then outward fixation is carried out from a periosteum layer through a supporting effect of a line carving product on the skin, so that multi-effect full-layer support of reinforcing steel bar and concrete is realized, and epidermal layer cells canbe activated, the spine layer is cleaned, the cell movement frequency of the base layer is improved, the updating and splitting speed of the base cells is activated, and the comprehensive anti-aging effect of different tissue layers is achieved.

The invention provides an immune cells movement speed detection method and a system thereof, which relate to the fields of biological and microbiological analysis detection. The method comprises the following steps: discontinuously obtaining several microscopic images containing immune cells; performing edge detection, filling up an internal gas of the edge to form a complete immune cells shape; calculating a change area of the immune cells at different time segments through a XOR operation, according to the change area and a perimeter of a change part, calculating an equivalent length; calculating an equivalent width according to the equivalent length and the change area; and calculating the immune cells movement deformation speed according to the equivalent width and time interval. Compared with the prior art, the immune cells movement speed detection method can be used for directly measuring the motion deformation speed of the immune cells, has the advantages of short measurement time, convenient operation, and no dyeing requirement, more importantly, the method has high measurement precision, is capable of performing quantitative analysis on the movement speed of the immune cells, and determines the activity of the immune cells.

The invention relates to a compound in a structure disclosed as Formula I, which can be used for preparing a Cdc42 inhibitor. Morphological analysis on filopodia, Western blot Cdc42 phosphorylation and downstream effector protein WASP analysis, cell wound healing test and formation test indicate that the compound provided by the invention can inhibit all processes into which Cdc42 participates, and effectively inhibit the action of Cdc42. The compound provided by the invention effectively inhibits cell functions into which actin participates, such as dictyosome tissue and cell movement.

The present invention relates to a composition for promoting stem cell activity, comprising a histone deacetylase inhibitor and a priming factor as active ingredients. When stem cells were treated with a low-concentration histone deacetylase inhibitor and priming factor, the activity of the stem cells significantly improved. In particular, primed MSCs promote a cell movement, colony-forming activity, and anti-inflammatory capability in terms of cell culturing conditions and thereby improve hypertension, inflammatory disease, or immune disease treatment effects and thus are expected to become auseful treatment strategy.

The invention discloses an ultrasonic physiotherapy device for muscular atrophy nursing based on an oscillating circuit device, and relates to the technical field of medical instruments. The ultrasonic physiotherapy device comprises the oscillating circuit device, a contact device and an ultrasonic generator. The ultrasonic physiotherapy device can increase the permeability of cells, stimulate cell movement, reduce edema, expand blood vessels, accelerate blood flow, improve blood circulation, increase blood vessel wall peristalsis, enhance cell membrane permeability, redistribute ions, make metabolism vigorous, reduce the concentration of hydrogen ions in tissues, increase the pH value, enhance enzyme activity and enhance tissue regeneration and repair capacity through slight massaging effects. Compared with mobile ultrasonic treatment, the device does not need to manually move an ultrasonic probe, is relatively low in operation difficulty, is suitable for household daily physiotherapy, does not generate local scorching feeling, is easy and painless in the physiotherapy process, and has a good physiotherapy effect on muscular atrophy symptoms.

The invention discloses a cell tracking statistical analysis system and a cell tracking statistical analysis method, and belongs to the field of data processing and image processing. The system comprises a parameter setting module, a secondary parameter-based multi-sample comparative analysis module, an origin normalized trajectory analysis module, and a trajectory tracking analysis module, wherein the parameter setting module is connected with the secondary parameter-based multi-sample comparative analysis module, the origin normalized trajectory analysis module and the trajectory tracking analysis module through data interfaces. According to the system and the method, the data acquired by target tracking software are subjected to effective, quick and flexible postprocessing in batches aiming at one or more selected samples, so that parameters which can describe cell movement characteristics intuitively are acquired, and related parameter curves and different types of cell movement trajectory are drawn for observing and comparing the difference of the cell movement characteristics of different samples.