14 results about "Cells movement" patented technology

The utility model discloses a cell separating and screening device and relates to the technical field of cell separation.The cell separating and screening device comprises a separating and screening box, side sliding grooves are formed in the two sides of the separating and screening box, cell preservation liquid flows out of an end through groove through the arrangement of the end through groove in an upward discharging mode, then the cell preservation liquid is gathered through a guide rod, and then the cell preservation liquid is separated and screened. The cell preservation liquid drips, the filtered cells are impacted, the gathered cells are dispersed, the cells are driven to move by utilizing the mobility of the cell preservation liquid, the screened cells can be screened out of the filtering frame, the screening effect is ensured, in this way, multiple times of screening are not needed, and the cell preservation liquid flows out from the upper portion through the design, so that the screening efficiency is improved. Separated cells can be prevented from entering a connecting pipe, waste is avoided, practicability is higher, a second driving motor is arranged to drive the connecting pipe to move, the cells are spread out, the utilization rate of the filtering frame is increased, and the filtering effect is guaranteed.

The utility model relates to the technical field of cell culture devices, in particular to a cell culture device for screening cell drugs, which comprises a base, a tank body, a vibration motor, a vibration component, an arc-shaped positioning block, a bidirectional adjusting screw rod, an arc-shaped clamping plate, an adjusting component, a blanking hopper, a rotating shaft, a scraper blade and a stirring component, a tank body is arranged above a base, a vibration motor is arranged at the top of the tank body, a vibration assembly is arranged on one side of the tank body, an arc-shaped positioning block is arranged on the outer side of the tank body, a two-way adjusting lead screw is arranged on one side of the tank body, an arc-shaped clamping plate is arranged on the outer side of the two-way adjusting lead screw, and an adjusting assembly is arranged on one side of the arc-shaped clamping plate. A discharging hopper is arranged on the bottom face of the tank, a rotating shaft is arranged on the inner side of the tank, a scraper is arranged on the outer side of the bottom end of the rotating shaft, and a stirring assembly is arranged on the outer side of the rotating shaft. By means of continuous vibration and wall-attached stirring, the cell movement speed is increased, cells are fully mixed, the cells are prevented from sinking to the bottom, and therefore the practical value of the device is enhanced.

The invention discloses a method and device for improving the single cell wrapping rate based on the acoustic flow control technology, and belongs to the field of the micro-nano technology, the device comprises a piezoelectric substrate, and the surface of the piezoelectric substrate is provided with a focusing interdigital transducer, an attached PDMS film and a micro-channel. The method comprises the following steps: dispersing cells in a water phase solution, injecting an oil phase and a water phase liquid with cells into a micro-channel, and adjusting the flow rate to form a stable laminar flow; cells flow in the water phase laminar flow, the interdigital transducer is turned on to generate a sound field, and the cells move towards an oil-water interface after reaching an action area and changing the track. The cell movement distance can be stably controlled by adjusting the voltage of the transducer, so that the cells penetrate through an interface to enter an oil phase to form effective wrapping. According to the invention, the cell encapsulation rate is improved based on the acoustic flow control technology, the traditional Poisson distribution limitation is broken through, and the method is expected to play an important role in the fields of digital ELISA, single cell analysis, biosensors and the like.

The invention relates to the technical field of biology, in particular to application of multi-ligand glycan-4 to regulation and control of intercellular function of macrophages and renal fibrosis. According to the application, the inhibitor for inhibiting the binding effect of the binding areas between the multi-ligand glycan-4 and the phagocytosis and cell movement protein 1 is used for preparing the accelerant for promoting the interburial function of the macrophages. The application is based on earlier-stage research finds that the platelet reaction protein 1 is combined with a multi-ligand glycan-4 receptor on the surface of macrophages, meanwhile, the platelet reaction protein 1 can promote combination between the multi-ligand glycan-4 and phagocytosis and cell movement protein 1, and the interburial function of the macrophages is inhibited. And an animal model of knocking down the multi-ligand glycan-4 can delay the progress of renal fibrosis. Therefore, the multi-ligand glycan-4 can regulate and control the cellular burial function of the macrophages and delay the process of renal fibrosis at the same time, and the blank that the cellular burial function of the targeted macrophages is applied to renal fibrosis treatment in the prior art is filled.

The invention discloses a micro-fluidic chip and a manufacturing method of the micro-fluidic chip. According to the micro-fluidic chip, the photoconductive area is formed between the at least two first supporting pieces, the photoconductive layer is arranged in the photoconductive area, light beams irradiate the lower polar plate, dielectrophoretic force is generated through the photoconductive layer to drive cells to move, and the cells can be effectively controlled; the supporting structure between the upper polar plate and the lower polar plate is divided into the first supporting pieces and the second supporting pieces, the at least two first supporting pieces are adjacently arranged, a microfluid channel is formed between the two adjacent first supporting pieces, and the second supporting pieces are used for forming auxiliary supporting between the upper polar plate and the lower polar plate, so that the stability of the micro-fluidic chip can be effectively improved; besides, the second supporting piece is arranged on the outer side of the first supporting piece, so that the movement of cells in the microfluid channel is not interfered, and a stable and uniform supporting effect can be provided on the outer side.

The invention provides a method for inducing mesenchymal stem cells to be differentiated into osteogenesis and application, and belongs to the technical field of stem cells. Comprising the following steps: a, preparing magnetic bioactive glass; b, constructing a polydopamine modified pearl powder composite porous hyaluronic acid-silk fibroin peptide scaffold; c, compounding the magnetic bioactive glass with the polydopamine modified pearl powder modified porous hyaluronic acid-silk fibroin peptide scaffold; d, coupling an induction compound to form a scaffold structure; and e, inoculating mesenchymal stem cells on the scaffold structure, pre-culturing, and culturing in an osteogenic induction culture medium to obtain osteoblasts. By constructing a scaffold structure, a suitable mechanical microenvironment is provided for stem cell osteogenic differentiation, cytoskeleton rearrangement is triggered, cell movement is driven, activation of differentiation signals is triggered, bone marrow mesenchymal stem cells are promoted to be differentiated to osteogenesis, and the scaffold has good application value.

The application provides a layout legalization checking and filler cell insertion method and device, equipment and medium, which are applied to the technical field of integrated circuit design automation, simulate filler cell insertion in the legalization stage of integrated circuit design, detect potential violation problems in advance before actual insertion of the filler cell, eliminate the violation through cell movement, realize mutual coordination between the legalization stage and the filler cell insertion stage, ensure that the physical circuit design does not violate ion implantation layer constraints after the filler cell insertion stage, thereby significantly improving the compliance of the overall design, and effectively shortening the design cycle while meeting the requirements of complex processes.

The present invention concerns a filter for use in a cell culture well, comprising: a ring sized to fit within the cell culture well; and a mesh attached to a planar side of the ring. The present invention also concerns a cell culture platform, comprising at least one cell culture well; a filter according to the invention wherein positioned within the at least one cell culture well, the filter partitions the at least one cell culture well into a first compartment and a second compartment; wherein the at least one filter is configured to allow fluid communication of a cell culture medium in both directions between both compartments and cell movement only from the first compartment to the second compartment. The invention belongs to the field of cell culture.

The invention provides a time sequence optimization method based on unit movement and incremental rewiring, which is used for improving the time sequence performance of a circuit through a heuristic local optimization strategy on the premise of not changing a logic netlist structure. The method comprises the following steps: firstly, preprocessing layout and wiring of a circuit, generating an initial wiring topology, and improving the quality of an initial solution through congestion-driven wiring reconstruction; then, the movable units are divided into a plurality of batches, a multi-thread parallel mechanism is adopted, a local rewiring algorithm based on A * search is executed in each small batch, and unit moving and wiring updating are completed; then pruning is carried out on the moving area based on the median frame principle, and the wiring efficiency and the gain evaluation precision are improved; and the wiring gain is estimated through a lookup table technology to assist in selecting candidate positions. For a redundant network structure generated after unit movement, a selective rewiring mechanism is introduced in the method, and local repair and optimization are carried out on related networks. In order to ensure the legality of the solution, a unit playback strategy based on wiring revenue and post-processing optimization driven by wiring length are finally adopted. Experimental results show that on the premise of keeping connectivity and rule constraints, the method obviously optimizes the negative time sequence margin (WNS / TNS) and the bus length, is suitable for time sequence optimization tasks after integrated circuit layout, and has good convergence and expandability.

A method of inducing migration of cells in a tissue comprises delivering a protein construct, or a nucleic acid encoding the protein construct, to cells of the tissue, and exposing a region of the tissue to light. Illumination of a region of the tissue can activate clustering of the light-inducible oligomerization domain of the protein construct within the cells that are exposed to light. Clustering can subsequently activate a signaling pathway that induces cell movement, e.g., cell migration. The directionality of cell migration, e.g., outward expansion of an edge of a tissue or inward migration resulting in tissue densification, is controlled by the position of the illuminated region with respect to the tissue.

The invention discloses a circulating tumor cell step-by-step separation device which comprises a material barrel, a separation assembly is arranged at the bottom end of the material barrel, the separation assembly comprises a plurality of spiral pipes connected to the bottom end of the material barrel through through holes, all the spiral pipes are jointly and uniformly arranged in the circumferential direction, and a center rod is coaxially connected to the bottom end of the material barrel; a fixing frame is arranged on the outer side of the spiral pipe, multiple sets of magnetic bead frames are arranged on the fixing frame and the center rod together, the set distance of each set of magnetic bead frames is equal to the screw pitch of the spiral pipe, separation magnetic beads are arranged on the inner side of each magnetic bead frame, and the inner sides of the magnetic bead frames make contact with the outer side of the spiral pipe; according to the separation assembly provided by the invention, circulating cells are separated step by step by arranging the spiral pipe, and the movement speed of the cells in the separation process can be reduced through the spiral arrangement, so that the situation that in a conventional separation mode, the movement speed of non-target cells is caused by vertical movement of the cells, and the target cells are separated in advance due to too large impact is avoided.

The application designs a global routing method considering advanced process constraints and cell movement, and belongs to the field of integrated circuit design automation, and mainly comprises the following steps: (1) for a given netlist with advanced process constraints and an initial layout routing solution, performing power-aware routing optimization; (2) constructing a routing layer constraint-aware lookup table; (3) based on the lookup table constructed in the previous stage, a timing-driven gain graph generation method is proposed; (4) determining the sequence of cell movement in the current round; (5) according to the cell movement sequence determined in the previous stage, performing cell movement and partial disconnection and redistribution of the line network; (6) performing cluster movement and partial disconnection and redistribution of the line network; (7) performing cell movement legalization and line network routing optimization. The application can effectively reduce the global routing line length based on the high-precision cell position prediction algorithm proposed in the method under the premise of considering advanced process constraints.

This invention proposes a chip standard cell legalization method and system considering the adjacency diffusion effect, relating to the field of electronic design automation. The specific scheme includes: constructing a minimum spanning tree based on cell layout and left / right diffusion height; decomposing and reconnecting the minimum spanning tree to generate Hamiltonian paths; calculating the cell's station position on the layout row according to the vertex order in the Hamiltonian path and the cell width; and designing an algorithm based on Hamiltonian completion to calculate the new cell position. By moving cells on a single layout row to make adjacent cells have the same diffusion height, this reduces NDE violations to near-optimal minimum and minimizes maximum cell movement without any area overhead, while achieving better actual runtime.

This invention discloses a pressing module for a double-glass module de-adhesive removal device, comprising a bottom mold and a pressing mold. The bottom mold has a top support surface flush with the top surface of the transfer roller. The pressing mold includes multiple pressing feet mounted side-by-side on the same bearing and rotatably pressed against the top support surface. The contact surface formed by each pressing foot slopes downward from the tip, with the tip close to the cutting module. On the one hand, based on the relative pressing assistance formed by the pressing mold and the bottom mold, and under the condition that the roller cutting direction of the cutting module and the moving direction of the module are the same, this application not only counteracts the stress caused by deformation or displacement of the module by the cutting module, but also optimally shortens the length of the free section to eliminate cutting safety hazards and reduce the damage rate caused by cell movement. On the other hand, each pressing foot adaptively adjusts its downward pressure based on the unevenness of the module surface, not only avoiding pulling caused by downward pressure, but also forming a squeeze that removes residual glass, thereby improving cutting quality.