The invention relates to a fluorescent quantitative detection kit for simultaneously detecting
human influenza A
virus,
human influenza B
virus and novel
coronavirus, and belongs to the technical field of
nucleic acid detection. The detection kit specifically comprises four groups of specific primer pairs and probes, a negative
quality control material, a positive
quality control material and a fluorescent quantitative PCR
reaction system, wherein the four groups of specific primer pairs and probes are respectively a pair of
specific primers and a probe for detecting
influenza A virus and influenza B virus, and two pairs of
specific primers and two probes for detecting novel
coronavirus; wherein the positive
quality control material is an artificially synthesized target sequence, and the negative quality
control material is deionized water; wherein the fluorescent quantitative PCR
reaction system is composed of components for PCR reaction and
reaction conditions. According to the kit disclosed by the invention, the specific primer pair and the probe are designed at a conservative site of a virus sequence; the kit can realize simultaneous detection of
influenza A virus, influenza Bvirus and novel
coronavirus in a
single tube, has strong detection specificity and high sensitivity, and can accurately and rapidly distinguish influenza from new coronapneumonia patients from people,so as to realize early discovery, early isolation and early treatment. The kit has important application value in the fields of
disease monitoring,
clinical diagnosis and the like.