138results about How to "Promote vascularization" patented technology

A reusable analyte sensor site for use with a replaceable analyte sensor for determining a level of an analyte includes a site housing and a resealable insertion site coupled to one end of the site housing. Preferably, the site housing is formed to have an interior cavity, and includes an outer membrane made of a material selected to promote vascularization and having a first pore size, and an inner membrane made of a material selected to be free of tissue ingress. Also, the site housing permits the analyte to pass through the site housing to the interior cavity to permit measurement by the replaceable analyte sensor. The resealable insertion site is provided for inserting the replaceable analyte sensor into the interior cavity of the site housing.

This invention relates to expression vectors comprising VEGF-D and its biologically active derivatives, cell lines stably expressing VEGF-D and its biologically active derivatives, and to a method of making a polypeptide using these expression vectors and host cells. The invention also relates to a method for treating and alleviating melanomas or tumors expressing VEGF-D and various diseases.

The present invention relates to the field of progenitor cells, and in particular to the field of cardiac progenitor cells. More particularly, the present invention pertains to the identification of a population of progenitor cells in the adult mammalian heart that is capable of giving rise to significant levels of de novo cardiomyocytes with the potential to replenish injured muscle post-infarction and/or promote neovascularisation to bring about complete cardiac regeneration. Accordingly, the present invention relates to methods for generating a population of mammalian post-natal epicardium derived cells (EPDCs), populations of EPDCs so generated, and methods of using same.

Apparatus for assaying an analyte in a body comprising: at least one light source implanted in the body controllable to illuminate a tissue region in the body with light at at least one wavelength that is absorbed by the analyte and as a result generates photoacoustic waves in the tissue region; at least one acoustic sensing transducer coupled to the body that receives acoustic energy from the photoacoustic waves and generates signals responsive thereto; and a processor that receives the signals and processes them to determine a concentration of the analyte in the illuminated tissue region.

The invention discloses a trace element-doped porous calcium carbonate ceramic, and a preparation method and application thereof, belonging to the field of medical materials for bone repair. The preparation method disclosed by the invention comprises the following steps: doping Mg, Sr, Zn, Si, Cu and other trace elements in a human body into calcium carbonate through a chemical precipitation method, or doping trace elements into low-temperature phosphate bioglass used as a sintering binder, uniformly mixing the trace element-doped calcium carbonate powder, the glass binder and pore forming agent, then forming, performing isostatic pressing treatment, sintering, and removing the pore forming agent to obtain the trace element-doped porous calcium carbonate ceramic. The trace element-doped porous calcium carbonate ceramic prepared by the invention has high strength and porosity, controllable degradation rate, long-term slow release of the doped trace element ions and favorable bone conductibility and inductivity, and is a novel artificially synthesized bone repair material.

The invention provides a collagen-based dura on the basis of a process-friendly type ion liquid on-site spinning technology, and a preparation method thereof. The preparation method comprises the steps of: dissolving I type collagen by a novel process-friendly solvent system-ion liquid and sodium salt composite system, fully soaking by taking decellularized pig dermis as a substrate through an electrolyte solution, carrying out electrostatic spinning by taking air as a medium, and regulating the pore structure of collagen fibers by adjusting the electrospinning conditions; removing residual ion liquid with a proper eluent, and carrying out freeze-drying, sterilization and packaging to obtain the collagen-based dura. The collagen-based dura has two or more layers of structures: the aperture of collagen fibers at one side close to brain tissue is controlled to be 3mu m and below, and the aperture of collagen fibers at one side far away from brain tissue is controlled to be 20mu m and above. Compared with the traditional solvent spinning, the preparation method is process-friendly, the toxic and side effects are reduced, and the triple-helical structure of the collagen can be furthest retained.

The invention relates to a porous bacterial cellulose skin repair material with a density structure and a preparation method thereof. The porous bacterial cellulose skin repair material with the 'upper dense and lower loose' structure similar to human skin is prepared by controlling the culture condition of bacterial cellulose and adding sustained-release microspheres in the culture process. A loose layer is tightly combined with a dense layer, so that obvious physical layering is avoided, and the structural continuity is good; and gradient changes of the structures are produced in the loose layer and the dense layer, and multiple pores distributed uniformly are formed in the loose layer, so that the upper dense and lower loose gradient structure of the human skin is simulated to the utmost degree. Cells easily enter the material, the healing period of a wound is obviously shortened, and proliferation of healed scars is effectively reduced; and good air permeability and water holding property of the bacterial cellulose are kept, the wound surface can be kept in a wet environment, and healing of the wound surface is facilitated. The forming process is simple, the culture period is short, the preparation process is environment-friendly, simple, convenient and quick, and the preparation cost is low.

The invention relates to an antibacterial biological activity stent and a preparation method thereof. The method comprises the steps that gelatin microspheres are prepared; porous gelatin microspheres are prepared; gelatin microspheres carried with active factors are obtained; an antibacterial stent is prepared, wherein the preparation of the antibacterial stent comprises the steps that at least one of collagen, chitosan and silk fibroin and nano-silver grains are added to acetic acid or malonic acid to prepare a mixed solution, the mixed solution is poured into a die which is horizontally paved with the solid gelatin microspheres, the die is rapidly placed in an ultra cold refrigerator for freezing treatment, and the die is frozen and dried to obtain the antibacterial stent; and the antibacterial biological activity stent is prepared by the steps that the gelatin microspheres carried with the active factors are placed into normal saline to prepare a suspension liquid, and the suspension liquid is poured into the antibacterial stent to obtain the antibacterial biological activity stent after dried at the room temperature. According to the antibacterial biological activity stent and the preparation method thereof, pure natural polymers are taken as raw materials, the nano-silver grains and the bioactive factors are compounded to prepare the stent, so that the stent has the biological activity and antibacterial capacity, can effectively promote vascularization of cambium and can be used for skin injury healing.

Methods and apparatus are disclosed for making and using adjustable epidermal tissue ingrowth cuff and catheter assemblies for transcutaneous placement to provide periodic or continuous external access for medical purposes to an interior body region of a patent who requires such medical treatment over an extended period of time.

The invention provides a method for manufacturing an artificial soft tissue body carried with a vascular net flow channel. The method comprises the following steps of: firstly designing a soft tissue scaffold model with a vascular structure, layering the soft tissue scaffold model one by one at equal distance, and manufacturing photomask plates of various layers; then uniformly mixing a cell and a collagen solution, and injecting a photocuring hydrogel and a photoinitiator to obtain a photocuring composite solution; injecting the photocuring composite solution to a work table, covering with the photomask plates, curing the photocuring composite solution by adopting a surface exposure technology, and then carrying out curing accumulation layer by layer to obtain a photocured hydrogel soft tissue scaffold with the vascular structure; planting vascular endothelial cells to the vascular structure of the photocured hydrogel soft tissue scaffold, wherein the vascular endothelial cells are attached to the surface of a vascular channel; carrying out static culture and dynamic culture in vitro to obtain the artificial soft tissue body carried with the vascular net flow channel. The method provided by the invention can solve the problems of survival of cells inside a large tissue engineering soft tissue scaffold, and soft tissue scaffold vessel network manufacturing and vascularization in the large injury repair of a soft tissue.

The containment and fixation system of the present invention generally includes a biomatix sleeve, biomatrix particles or combinations thereof made of a biomatrix material. The biomatrix material is comprised of one or more biocompatible proteins and one or more biocompatible solvents. The biomatrix material utilized in the sleeve and/or particles may also include one or more pharmacologically active agents like therapeutic biochemicals such as a bone mending biochemical (e.g. hydroxyapatite) or an angiogenic growth factor (e.g. BMP).

The present invention discloses a calcium magnesium silicate porous ceramic ball ocularprosthesis seat and a preparation method. The calcium magnesium silicate porous ceramic ball ocularprosthesis seat comprises a non-biodegradable calcium magnesium silicate porous ceramic ball ocularprosthesis seat and a degradable calcium magnesium silicate modification layer covering the surface of the pore channel wall, wherein the ocularprosthesis seat has a completely penetrating porous structure, the porosity is 35-85%, the pore channel diameter is 60-800 [mu]m, the ocularprosthesis seat is a porous diopside ceramic ball constructed by using a three-dimensional printing technology, and the pore channel wall is subjected to degradable calcium magnesium silicate gel precursor filling modification and secondary sintering to obtain the product. According to the present invention, after the calcium magnesium silicate porous ceramic ball ocularprosthesis seat is implanted into the eye socket, the neovessel growth is promoted through the bioactivity of the pore channel surface layer calcium magnesium silicate layer so as to achieve the rapid vascularization in the pore channel and avoid the displacement or prolapse of the ocularprosthesis seat; and the calcium-silicon-based ceramic ocularprosthesis seat pore channel wall bioactivity is excellent, and the application value is provided in the reconstruction of the ocular base.

The invention relates to the technical field of tissue engineering and medical wound repairing, in particular to an amnion innate stem cell carried frozen active amnion particle and a conditioned medium and application thereof. Discarded fresh amnions are prepared into particles and then frozen into liquid nitrogen by means of serum-free stem cell freezing liquid; thus, complete matrix components of amnion are maintained while amnion epithelial cell activity is maintained for a long time, and further, disease transmission is avoided effectively. The conditioned medium which is prepared and collected by means of the frozen active amnion particles can promote chemotaxis and migration of human epidermal cells, fibroblast and endothelial cells effectively. In terms of zoografting, wound healing can be improved by multiple ways of regulating inflammatory reaction, promoting vascularization, quickening epithelization and the like through the active amnion particles; further, the amnion matrix can be directly used as a dermis equivalent to induce dermal regeneration so as to improve wound healing quality remarkably. The amnion innate stem cell carried frozen active amnion particle and the conditioned medium thereof can provide simple but effective ways for wound repairing.

Tissue engineering devices and methods employing scaffolds made of absorbable material for use in the human body for tissue genesis and regenerative and cellular medicine including breast reconstruction and cosmetic and aesthetic procedures and supplementing organ function in vivo.

The invention relates to a regeneration material of artificial dermis substitution and a preparation method thereof. In the regeneration material of a dermis substitution for tissue engineering skin for loading rhGM-CSF, the regeneration material is formed by a heparinizing collagen-chitosan bracket loading the rhGM-CSF solution. The invention further discloses a preparation method of the regeneration material. The invention provides an artificial dermis substitution with good performance for curing the whole skin coloboma of trauma, burning, chronic skin ulcer, thereby being capable of remarkably quickening the vascularization process of the regeneration material of artificial dermis substitution, decreasing the infection risk, promoting the healing of the surface of a wound and reducing the hyperplasia of scar, and thus easing the pain of patients. The invention can be widely applied for the aspects of trauma, burning, surgery reshaping, and the like. The preparing method is simple, the source of the material is wide, the production efficiency is high, and the invention is suitable for industrial production.

The invention discloses a dual-phase magnetic nano-composite scaffold material. The dual-phase magnetic nano-composite scaffold material is prepared by compositing a cartilago phase with a bone phase,wherein the cartilago phase contains polylactic acid and a natural polymer compound; and the bone phase contains polylactic acid, nano-hydroxyapatite and magnetic nanoparticles. The invention furtherdiscloses a preparation method of the dual-phase magnetic nano-composite scaffold material. The three-dimensional dual-phase magnetic nano-composite scaffold material is prepared from polylactic acid, the natural polymer compound, nano-hydroxyapatite and the magnetic nano particles by virtue of a low-temperature rapid forming technique and is integrated with the advantages of the four materials,is capable of promoting the adhesion and proliferation of cells, reducing the toxicity of degradation products and improving the biomechanical properties based on good osteoconduction and biocompatibility and is relatively beneficial to the adhesion growth and vascularization of solid cells, and the speed and effect of the coalescence between artificial cartilages transplanted at bone defect partsof a joint cartilage and a subchondral bone and the bones are greatly increased and improved.