Composite material of organic/inorganic multi-phase induction nano-hydroxyapatite

A nano-hydroxyapatite and composite material technology, applied in medical science, prosthesis, tissue regeneration, etc., can solve the problem that nanoparticles cannot be well dispersed, improve biomechanical properties, and is conducive to adhesion growth and vascularization, the effect of simple process

Inactive Publication Date: 2016-11-09
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above reports have the following defects: after the synthesis of hydroxyapatite nanoparticles, they are added to the precursor solution, so that the nanoparticles cannot be well and evenly dispersed on the scaffold

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Dissolve 1.0 g of chitosan in 1 mL of acetic acid with a volume fraction of 2% to obtain an acetic acid solution of chitosan; dissolve 8 g of bovine collagen in 8 mL of secondary water at room temperature and stir for 2 d to obtain bovine collagen Aqueous solution

[0031] (2) Under magnetic stirring, add the aqueous solution of bovine collagen to the acetic acid solution of chitosan and stir for 0.5h to make it fully mixed and uniform;

[0032] (3) Slowly add 2mL, 8mg / mL graphene oxide aqueous solution, and stir for 0.5h to form a homogeneous mixture;

[0033] (4) Add 3mL, 2mol / L soluble calcium salt solution and stir for 30min, then add 3mL, 1.2mol / L soluble phosphate solution and stir for 4h, stir well to make it evenly mixed;

[0034] (5) Add 4.5mL, 0.04g / mL, EDC and 5.6mL, 0.01g / mL, NHS mixed crosslinking agent. After crosslinking at room temperature for 4h, transfer the mixture to the mold and pre-frozen at 4℃ for 5h , Then freeze-drying at -80°C for 12h; continue fr...

Embodiment 2

[0037] (1) Weigh 1.15g of chitosan and dissolve it in a 1% acetic acid solution to obtain an acetic acid solution of chitosan; dissolve 8.5g of bovine collagen in 9mL of secondary water and stir for 2.5d at room temperature. Obtain an aqueous solution of bovine collagen;

[0038] (2) Under magnetic stirring, add the aqueous solution of bovine collagen to the acetic acid solution of chitosan and stir for 0.5h to make it fully mixed and uniform;

[0039] (3) Slowly add 4mL, 7mg / mL graphene oxide aqueous solution, and stir for 0.5h;

[0040] (4) Slowly add 2.4mL, 2.5mol / L soluble calcium salt solution and stir for 30min, then add 2.4mL, 1.5mol / L soluble phosphate solution and stir for 4h, stir well to make it evenly mixed

[0041] (5) Add 6.7mL, 0.03g / mL, EDC and 8.3mL, 0.02g / mL, NHS mixed crosslinking agent. After crosslinking at room temperature for 6 hours, transfer the mixed solution to the mold and pre-frozen at 2°C for 5 hours. , Then freeze-drying at -70°C for 12h; continue freez...

Embodiment 3

[0044] (1) Weigh 1.25 g of chitosan in an acetic acid solution with a volume fraction of 1.5% to obtain an acetic acid solution of chitosan; dissolve 9 g of bovine collagen in 10 mL of secondary water, stir at room temperature for 3 days, and obtain bovine Aqueous solution of collagen;

[0045] (2) Under magnetic stirring, add the aqueous solution of bovine collagen to the acetic acid solution of chitosan and stir for 0.5h to make it fully mixed and uniform;

[0046] (3) Slowly add 8mL, 6mg / mL graphene oxide aqueous solution, and stir for 0.5h;

[0047] (4) Slowly add 2mL, 3mol / L soluble calcium salt solution and stir for 30min, then add 2mL, 1.8mol / L soluble phosphate solution and stir for 4h, stir well to make it evenly mixed

[0048] (5) Add 5.6mL, 0.02g / mL, EDC and 7mL, 0.03g / mL, NHS mixed crosslinking agent. After crosslinking at room temperature for 8h, transfer the mixed solution to the mold and pre-frozen at 3℃ for 4h. Then freeze-drying at -10°C for 12h; continue freeze-dryi...

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Abstract

The invention discloses a composite material of organic / inorganic multi-phase induction nano-hydroxyapatite. The composite material is characterized in that on the basis of the effect that graphene oxide can enhance adhesion of stem cells, and stem cells are induced to be differentiated into bone cells and adsorb organic and inorganic nanoparticles, chitosan and bovine collagen are adopted as organic matrixes, a graphene oxide water solution is adopted as an inorganic matrix, a soluble calcium salt and a soluble phosphate are adopted as a precursor of inorganic phase nano-hydroxyapatite, a biological mechanism and an in-situ composite preparation technology are adopted, and the composite material of organic / inorganic multi-phase induction nano-hydroxyapatite is prepared bionically. The preparation conditions are mild, the composite material is uniform in pore diameter, the hole forming performance is good, the biocompatibility and biodegradability are good, and the composite material is expected to be the novel composite material for treating osteoporosis.

Description

Technical field [0001] The invention belongs to the field of composite materials, and specifically relates to a composite material of organic / inorganic multiphase induced nano-hydroxyapatite. Background technique [0002] As the life expectancy of the population continues to increase and the elderly population continues to increase, osteoporosis and osteoporotic fractures, which are one of the degenerative diseases of the elderly, have caused more and more elderly people to suffer. Suffered long-term physical pain, disability and even died prematurely. Many people are familiar with the name osteoporosis. As the name suggests, the density of bone is reduced, as if wood is rotten, and there are many gaps in the bones, so that the toughness and strength of the bone are reduced, and the quality of the bone is also reduced. Decrease, increase the fragility of the bone and easily fracture. Osteoporosis can be divided into three categories: one is primary osteoporosis, which is a phys...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/46A61L27/44A61L27/42A61L27/50A61L27/56A61L27/58
CPCA61L27/46A61L27/427A61L27/446A61L27/50A61L27/56A61L27/58A61L2430/02C08L5/08C08L89/00
Inventor 陈景帝范田堂刘小翠胡义敏张其清
Owner FUZHOU UNIV
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