45 results about "Hydroxyapatite nanoparticles" patented technology

The invention discloses a preparation method of hydroxyapatite-artificial bear gall powder nanoparticles, the preparation method comprises the steps of preparing hydroxyapatite nanoparticles and loading artificial bear gall powder, the preparation method is simple and easy to implement, mild in condition and suitable for drug loading, and the preparation method is suitable for large-scale industrial production. The prepared nano-particles have good biocompatibility, degradability, high safety, high stability and high aqueous medium dispersibility, and due to the stability, the nano-particles can realize long-time circulation of drugs in vivo and avoid premature removal of the drugs, so that the bioavailability of the drugs is improved; the obvious pH-responsive slow release of the artificial bear gall powder can be realized, so that the artificial bear gall powder can be used in targeted therapy; compared with other carrier materials, the adopted raw materials are low in price, do not contain metal, have great advantages in cost and environmental protection, are easier to realize large-scale industrial production, and have good application prospects.

The invention belongs to the technical field of biomedical materials, and particularly relates to composite hydrogel as well as a preparation method and application thereof. The composite hydrogel comprises a thermosensitive forward gel material, a thermosensitive reverse gel material, a biological material, an initiator and a hemostatic component, the thermosensitive positive gel material is prepared from at least one of hydroxybutyl chitosan and poly (N-isopropylacrylamide); the thermosensitive reverse gel material comprises at least one of methacrylated gelatin and double-bond sodium hyaluronate; the biological material comprises at least one of hydroxyapatite nanoparticles and bioglass. The composite hydrogel disclosed by the invention has double-temperature threshold thermal responsiveness, can be quickly gelated at the temperature of less than 18.6 DEG C or greater than 24.3 DEG C to construct a physical hemostasis barrier, can maintain a flowing state at the temperature of 18.6-24.3 DEG C, and has good injectability and controllability. Meanwhile, by combining biological materials and hemostatic components, good hemostatic and bone defect repairing effects are achieved.

The invention provides a foam tape and a preparation method thereof, and relates to the technical field of adhesives, the foam tape comprises the following components by weight: 50-80 parts of a propylene pressure-sensitive adhesive, 10-20 parts of a foaming agent, 1-5 parts of a cross-linking agent, 0.5-2 parts of an antioxidant, 5-15 parts of a diluent, 1-10 parts of hydroxyapatite nanoparticles, 5-15 parts of bio-based polyamide, and 2-8 parts of flake alumina. According to the invention, due to the addition of the hydroxyapatite nanoparticles, the wear resistance, adhesive force and biocompatibility of the adhesive tape are improved, the adhesive tape is safer and more reliable in contact with a human body, and meanwhile, the strength and durability of the adhesive tape are also enhanced. And secondly, due to the introduction of bio-based polyamide, the toughness and heat resistance of the adhesive tape are improved, meanwhile, the dependence on a traditional petrochemical-based material is reduced, and the characteristics of environmental protection and sustainable development are embodied.

The invention provides a composite sun-screening agent based on an encapsulation technology as well as a preparation method and application of the composite sun-screening agent. The preparation method of the composite sun-screening agent comprises the following steps: fully dispersing hydroxyapatite nanoparticles in an alkaline solvent, adding a dopamine monomer, and carrying out oxidative polymerization reaction, centrifugal washing and drying to obtain HAP-PDA composite nanoparticles; and fully dispersing the HAP-PDA composite nanoparticles in a solvent, adding a mixed solution of a chemical sun-screening agent and an antioxidant and a surfactant, and performing encapsulation reaction and centrifugation to obtain the product. According to the invention, a polydopamine and hydroxyapatite composite encapsulation technology is innovatively adopted, so that the stability and biocompatibility of the sun-screening agent and the antioxidant are remarkably improved, the sun-screening effect is enhanced, and the product is endowed with a lasting sun-screening effect and excellent oxidation resistance. According to the invention, the limitation of a traditional sunscreen product is broken through, and safer, more efficient and more comfortable sunscreen experience can be provided for consumers.

The invention relates to the field of food packaging, and particularly discloses a food packaging sheet and a preparation process thereof. Comprising a composite modified PP micro-foaming sheet layer, and the composite modified PP micro-foaming sheet layer comprises the following raw materials in parts by weight: modified PP particles; hydroxyapatite nanoparticles; a hydrogen peroxide solution; a silane coupling agent; a foaming agent; the modified PP particles are obtained by grafting collagen on the surfaces of the PP particles; the preparation process comprises the following steps: S1, preparing the inner surface layer and the outer surface layer; s2, preparing a mixed material A; s3, preparing a PP sheet material; s4, preparing the composite modified PP micro-foaming sheet material; s5, co-extruding the PP sheet and the composite modified PP micro-foaming sheet to prepare an intermediate sheet; and S6, immersing the intermediate sheet into an SBF solution, oscillating at constant temperature, and drying to obtain the food packaging sheet. The PP micro-foaming sheet can be used for food packaging and has the advantage of high toughness; in addition, the preparation technology has the advantage that the toughness and strength of the food packaging sheet are further improved.

The invention discloses a gradient degradation type personalized absorbable sternum fracture fixing plate and a preparation process, and belongs to the technical field of medical implant materials. Comprising an anatomical adaptive plate body and an integrated locking assembly, the plate body is of a partitioned structure of a middle supporting area and an edge buffering area, a gradient pore array penetrating through the thickness direction is formed in the surface of the plate body, and a hydroxyapatite-collagen composite coating is loaded on the inner wall of a pore; the plate body is made of a composite absorbable material through 3D printing forming and hot pressing post-treatment, and the composite absorbable material comprises, by weight, 60%-70% of polylactic acid-glycolic acid copolymer (PLGA, LA / GA = 75 / 25), 15%-20% of silanized magnesium alloy microwires, 5%-10% of hydroxyapatite nano-particles and 5%-10% of polycaprolactone (PCL). Space-time matching of the degradation rate and the bone healing process is achieved through the partition gradient structure, the magnesium alloy microwires enhance the mechanical property and release active ions to promote osteogenesis, and the method has the advantages of personalized adaptation, gradient degradation, bone regeneration promotion and the like.

This invention belongs to the field of biomedical materials technology, specifically disclosing a composite material for promoting the repair of soft tissue defects in the maxillofacial region and its preparation method. The composite material comprises the following raw materials: oxidized hyaluronic acid, carboxymethyl chitosan, dopamine-functionalized hydroxyapatite nanoparticles, strontium salt, copper salt, glycerol, and deionized water. The preparation method includes: firstly, strontium salt and copper salt are hierarchically loaded onto the surface of dopamine-functionalized hydroxyapatite nanoparticles via a dual mechanism of "lattice substitution-surface coordination," and then, together with oxidized hyaluronic acid and carboxymethyl chitosan, construct a "dynamic covalent-hydrogen bond-coordination bond" triple synergistic network through Schiff base reaction, hydrogen bonding, and coordination bonds. This invention solves the technical problems of poor wet surface adhesion, low mechanical strength, single function, and easy burst release of ions in existing repair materials, exhibiting excellent effects such as sequential antibacterial and repair-promoting properties, high adhesion strength, and long-lasting sustained release.

The invention discloses a rapid-absorption collagen repair membrane and a preparation method thereof, and relates to the technical field of biological materials. The repairing membrane is of a double-layer structure and comprises a purified collagen layer and an absorbable polymer spinning layer loaded with inorganic nanoparticles. The preparation method comprises the following steps: purifying animal skin to obtain a purified collagen layer; the preparation method comprises the following steps: respectively dispersing akermanite and hydroxyapatite nanoparticles, and mixing; adding the mixed dispersion liquid into the absorbable polymer solution to obtain a composite spinning solution; spinning the composite spinning solution on the surface of the collagen layer by adopting an electrostatic spinning method to form a spinning layer; and drying to obtain the repairing film. According to the invention, by regulating and controlling the variety, proportion, crystallinity and spatial distribution of the inorganic particles, the synergistic enhancement of mechanical properties and biological activity is realized, so that the repair film has pH-responsive graded release ability, can automatically adjust degradation behavior according to inflammation intensity, actively adapts to microenvironment, and has good application prospects. The method can be widely applied to the fields of oral soft tissue repair, periodontal wound covering and the like.

The invention discloses a photo-thermal response type polyether-ether-ketone nano composite material. A preparation method of the photo-thermal response type polyether-ether-ketone nano composite material comprises the following steps: 1) continuously reacting nano hydroxyapatite with dopamine hydrochloride in a stirring state to prepare polydopamine coated hydroxyapatite nano particles; 2) dispersing the polyether-ether-ketone micro powder and the polydopamine-coated hydroxyapatite nanoparticles in water, and carrying out preliminary blending, separation, freeze drying, drying, crushing and high-speed ball milling to obtain uniform composite powder; and 3) carrying out extrusion molding on the obtained composite powder to obtain the photo-thermal response type polyether-ether-ketone nano composite material. The preparation method comprises the following steps: firstly, preparing polydopamine-coated hydroxyapatite nano-particles by adopting an in-situ polymerization method, and preparing the photo-thermal response type polyether-ether-ketone and polydopamine-coated hydroxyapatite nano-particle composite material by combining processes such as ball-milling blending and melt extrusion, so that good mechanical properties, biocompatibility, photo-thermal response performance and the like can be considered; and the adaptability is wide.

The invention discloses a micron-sized gelatin-hydroxyapatite composite fiber material and a preparation method thereof. The preparation method comprises the following steps: (a) dissolving or dispersing gelatin in a volatile acid solution to obtain a gelatin solution; adding a cross-linking agent into the gelatin solution for in-situ cross-linking, and then adding hydroxyapatite nanoparticles for dispersion to obtain a mixed solution; the mass ratio of the hydroxyapatite nano particles to the gelatin is 0.30 to 1.50; (b) performing centrifugal spinning on the mixed solution, and drying to obtain a gelatin-hydroxyapatite composite fiber precursor; and (c) carrying out secondary crosslinking treatment on the gelatin-hydroxyapatite composite fiber precursor to obtain the gelatin-hydroxyapatite composite fiber material. The interference of HA particles on an electric field can be avoided, the uniformity of fibers is ensured, the production efficiency is higher, and the method is suitable for large-scale industrial production; by optimizing the formula of the mixed solution, the bone conduction performance of the composite fiber is effectively enhanced.

The invention relates to the field of bone tissue engineering and regenerative medicine, in particular to non-denatured bone protein calcium superfine powder with bone tissue oriented growth and rapid repair effects and a preparation method and application of the non-denatured bone protein calcium superfine powder, and the non-denatured bone protein calcium superfine powder comprises the following components in percentage by weight: 25%-45% of non-denatured type I collagen, 0.005%-0.05% of bone morphogenetic protein-2, 0.001%-0.02% of transforming growth factor-beta1, 0.01%-0.02% of calcium sulfate, 0.01%-0.02% of calcium sulfate, 0.01%-0.02% of calcium The bone tissue repairing agent is prepared from, by weight, 0.001%-0.02% of basic fibroblast growth factors, 20%-40% of ultramicro hydroxyapatite nanoparticles, 5%-15% of chitosan oligosaccharide, 3%-10% of mannitol calcium complex, 2%-8% of sodium hyaluronate, 0.01%-0.1% of vitamin D3, 0.01%-0.1% of vitamin K2, 5%-15% of chondroitin sulfate, 1%-4% of casein phosphopeptides and the balance auxiliary materials, and directional growth and rapid repairing of bone tissue are promoted.

The invention discloses a naringin carbon quantum dot-based bone-targeting nanocomposite as well as a preparation method and application thereof. The preparation method comprises the following steps: 1, preparing naringin-derived carbon quantum dots (N-CQDs); 2, preparing hybrid silicon dioxide / hydroxyapatite nano particles MH; (3) preparing amino functionalized MH nano particles MH-NH2; 4, adding the activated N-CQDs solution into the MH-NH2 solution, and carrying out stirring reaction at room temperature to prepare MH-CQDs; and 5, dispersing the NHS-PEG-Mal into the MH-CQDs solution, slowly stirring, and dialyzing after the reaction is completed, so as to obtain the P-coated MH-CQDs. According to the nano-composite disclosed by the invention, a natural drug naringin with osteogenic activity is creatively converted into N-CQDs with a pharmacological function and a fluorescent tracing capability, and the N-CQDs are combined with a bionic silicon dioxide-hydroxyapatite substrate, so that the essential fusion of a'drug-probe 'function is realized.

The invention relates to the field of liposome manufacturing, and discloses an amphotericin B liposome and a preparation method thereof, and the amphotericin B liposome comprises the following components in parts by mass: 1 part of amphotericin B, 5-10 parts of hydrogenated soybean phospholipid (HSPC), 2-4 parts of cholesterol, 1-3 parts of mPEG2000-DSPE, 0.5-1.5 parts of ceramide, 0.3-0.8 part of glycyrrhetinic acid, 0.2-0.5 part of hydroxyapatite nanoparticles (nHAP) and 8-15 parts of a freeze-drying protective agent. The glycyrrhetinic acid is added, and the specific targeting effect of the glycyrrhetinic acid is utilized, so that the in-vitro binding rate of fungal cells of the lipidosome is improved, the enrichment rate of the medicine at an infected part is further improved, and the core problem of renal toxicity caused by non-targeting distribution of a traditional preparation is effectively solved; the hydroxyapatite nanoparticles adsorb medicine molecules to form a medicine-nHAP compound, and the medicine crystallization rate is greatly reduced by combining the synergistic anti-crystallization effect of the freeze-drying protective agent.

The present invention relates to a silk fibroin / hydroxyapatite composite material, and a preparation method therefor and an application thereof. A uniform mixture of hydroxyapatite nanoparticles, silk fibroin, and hexafluoroisopropanol is maintained at a temperature range from 50° C. to 60° C. for at least 1 hour to obtain a silk fibroin / hydroxyapatite solution; the silk fibroin / hydroxyapatite solution is poured into a long cylindrical mold, two ends of the long cylindrical mold being respectively an end A and an end B; at room temperature, the end A is opened and the end B is closed, and the mold is vertically immersed in methanol with the opening facing up and let stand for at least 2 days; the mold is turned upside down, the end A is closed and the end B is opened, and the mold is vertically immersed in the methanol with the opening facing up and let stand for at least 2 days; the mold is removed, and ventilation and drying are performed to obtain a silk fibroin / hydroxyapatite composite material. The silk fibroin / hydroxyapatite composite material prepared in the present invention has uniform strength, good mechanical properties, and significant osteoinductivity.

The invention discloses injectable hydrogel for treating periodontitis as well as a preparation method and application of the injectable hydrogel. The injectable hydrogel is prepared from the following raw materials: chitosan and derivatives thereof, oxidative modified natural polysaccharide and modified hydroxyapatite, the modified hydroxyapatite comprises hydroxyapatite, and the surface of the hydroxyapatite is modified with a metal polyphenol network. The modified hydroxyapatite has multiple functions of oxidation resistance, immunoregulation, osteogenesis promotion, migration promotion, tube formation promotion, antibiosis and the like, and can be combined with the injectable hydrogel with biological adhesion and self-healing performance to realize better in-vivo tissue healing. The injectable hydrogel of the composite functional hydroxyapatite nanoparticles synthesized by the invention has biocompatibility, oxidation resistance, immunoregulation performance, osteogenesis promotion performance and antibacterial performance, and can realize better alveolar bone regeneration and repair in a rat periodontitis model, and the effect of the injectable hydrogel is superior to that of clinically common minocycline.

The invention provides a magnetic artificial periosteum with a micro-nano multilevel structure and a preparation method and application of the magnetic artificial periosteum, and belongs to the technical field of biomedical material development. The bionic periosteum with the micro-nano multilevel structure is successfully obtained through an elaborately designed preparation process by fusing active ingredients of Chinese herbal medicines and the polysaccharide high polymer material alkaline solution, and the problem that in the prior art, the performance of a bone defect repair material is limited is solved. The preparation method provided by the invention is simple and convenient to operate and easy to control and implement, and the obtained magnetic bionic periosteum structurally shows excellent mechanical properties and more excellent biological activity. In addition, magnetic hydroxyapatite nanoparticles are also introduced, so that the bionic periosteum can have a synergistic effect with an external magnetic field, the magnetic biological effect is exerted, bone defect repair is remarkably promoted, and the overall efficiency of the product is further improved.

The invention relates to the technical field of clothing mildew prevention, in particular to a food-grade mildew-proof gel bead and a preparation method thereof, and the food-grade mildew-proof gel bead comprises an inner core and an outer side coating layer coating the inner core; the inner core is prepared from a modified natural mildew preventive, hydroxyapatite nanoparticles, edible gelatin, microcrystalline cellulose and water; the outer coating layer is prepared from carrageenan, konjac glucomannan, glycerol and water; the food-grade mildewproof gel bead provided by the invention shows remarkable advantages in the aspects of mildewproof performance, structural stability, application adaptability and the like through a multi-component synergistic effect. The modified natural mildewproof agent in the inner core of the prepared mildewproof gel bead inhibits growth of mould through a dual mechanism: amino on a chitosan molecular chain carries positive charges and can be adsorbed on the surface of a mould negative charge cell membrane, the membrane integrity is destroyed, and nutrient absorption is blocked; kappa-carrageenan forms a gel network to delay component release, so that antibacterial components continuously take effect for a long time, and the antibacterial rate is relatively high.

The invention relates to the technical field of membrane separation, in particular to a nanofiltration membrane and a preparation method thereof.The method comprises the steps that the surface of a porous supporting membrane is sequentially impregnated with a hydroxyapatite nanoparticle water-phase dispersion liquid containing a polyamine monomer and a polyacyl chloride monomer organic-phase solution; generating a polymer on the surface of the porous support membrane through a polymerization reaction of a polyamine monomer and a polyacyl chloride monomer; the polymer is used as an active separation layer, and the porous support membrane and the active separation layer are used as nanofiltration membranes. According to the invention, the nano polyamide mixed matrix nanofiltration membrane is generated on the porous support membrane through the polyamine monomer and the polyacyl chloride monomer. The hydroxyapatite nanoparticles and the polyamide matrix are combined through chemical bonds, so that the permeability and fluorine ion selectivity of the nanofiltration membrane are improved, and meanwhile, the membrane stability is remarkably improved.

The invention discloses an anti-inflammatory and mineralization-promoting composite pith capping agent based on apoptosis vesicles and a preparation method and application thereof, and belongs to the technical field of biomedical materials. According to the pith capping agent, apoptosis vesicles, hydroxyapatite nanoparticles and methacrylated gelatin are combined, the apoptosis vesicles provide a stable anti-inflammatory effect, the hydroxyapatite nanoparticles promote moderate mineralization, and the methacrylated gelatin serves as a carrier matrix to achieve substance slow release. According to the preparation method, the process and the component adding sequence are optimized, the nano-particles and the gelatin are uniformly mixed firstly, and then the apoptosis vesicles are introduced, so that the problems of poor stability and function integration of the apoptosis vesicles are solved. In application, the three components cooperate to realize dynamic balance of inflammation inhibition and mineralization promotion, pulpitis is effectively controlled, excessive calcification is avoided, an ideal microenvironment is provided for dental pulp tissue repair, and the defects of existing materials in pulpitis control and mineralization balance are overcome.

The invention discloses a 3D printing rapid curing method suitable for a hydrogen-bond-rich polymer and a biological scaffold of the hydrogen-bond-rich polymer. The 3D printing rapid curing method comprises the following steps: step 01, preparing direct-writing printing ink containing the hydrogen-bond-rich polymer and a solvent; 02, the ink is used for constructing a preset structure through direct-writing type three-dimensional printing; and step 03, placing the predetermined structure constructed in the step 02 in a substitute solvent which is mutually soluble with the solvent but does not dissolve the polymer to realize polymer precipitation and solidification. After printing is completed, a predetermined structure is immediately soaked in a high-polarity alternative solvent (such as water), and an original printing solvent is rapidly released by utilizing a hydrogen bond reconfiguration principle, so that rapid precipitation and forming of a polymer are promoted. The nano-particles provided by the invention can promote solvent diffusion: the hydroxyapatite nano-particles are introduced into the ink to construct an internal micro-turbulence and diffusion channel, so that the solvent evaporation rate is remarkably improved, and meanwhile, the strength, the biological activity and the osteogenesis performance of the scaffold are improved.

The present application relates to a kind of β-nicotinamide mononucleotide encapsulated hydroxyapatite nanoparticles (NMN-HAP) and its preparation method and application. Specifically, the nanoparticles include β-nicotinamide mononucleotide and hydroxyapatite. The preparation method of the NMN-HAP includes the following steps: hydroxyapatite is mixed with pure water and dispersed, then pure water in the suspension is replaced with ethanol, and β-nicotinamide mononucleotide aqueous solution is added;After vortex incubation of the mixture, remove the supernatant. The NMN-HAP provided in the present application has the potential to improve the bioavailability of NMN and in vivo NAD + anti-aging potential while maintaining bone health, providing potential for dual health benefits.

The application discloses a kind of based on HAP nano-modified composite fiber filter membrane and its preparation method and application, it is related to the field of insulating oil filtration processing.Preparation method is in hydroxyapatite nanoparticle join alkali solution, 3-glycidyloxypropyl trimethoxysilane reaction preparation surface-modified hydroxyapatite nanoparticle, subsequently with PTFE powder, dispersant is mixed and dispersed to obtain spinning solution, composite fiber filter membrane is prepared using electrostatic spinning machine.The composite fiber filter membrane prepared in the application is doped with surface-modified hydroxyapatite nanoparticle, can efficiently adsorb aging acidic substances in vegetable insulating oil, meanwhile, the filter membrane is intercepted and captured to other micro-impurities, effectively reduces the impurity content in vegetable insulating oil to realize purification, so as to reduce the dielectric loss factor and viscosity of vegetable insulating oil, improve aging life and insulation performance.

The invention discloses iron tailing powder-slag powder co-doped concrete and a preparation method thereof, and belongs to the technical field of concrete materials. The invention aims to solve the problem that concrete for an intertidal zone bank protection structure is difficult to adapt to composite working conditions of high-salt soaking, tidal scouring, wave impact and silt abrasion. The concrete is prepared from the following components in parts by mass: 30 to 40 parts of P.O 42.5 ordinary Portland cement, 35 to 50 parts of modified iron tailing powder, 15 to 28 parts of superfine slag powder, 2 to 6 parts of steel slag-based nano calcium-lignosulfonate grafted composite powder, 2.0 to 4.0 parts of desulfurized gypsum, 1.0 to 3.0 parts of lithium slag powder, 0.5 to 1.5 parts of hydrated calcium aluminate, 0.8 to 2 parts of modified hydroxyapatite nano powder and 105 to 130 parts of modified iron tailing aggregate, 0.4-1.2 parts of basalt fibers, 0.7-1.5 parts of a polycarboxylic acid type high-efficiency water reducing agent, 0.1-0.5 part of a modified konjac glucomannan-polyether amine copolymer and the like. Through the synergistic effect of multiple components, intertidal zone composite working condition erosion can be effectively resisted, the long-term service requirement of a revetment structure is met, and meanwhile the resource utilization rate of solid waste such as iron tailings is increased.

The invention relates to the technical field of dental and orthopedic implants, in particular to a zirconia osseointegration implant material and a preparation method thereof. Aiming at the problems of strong biological inertness and slow early-stage osseointegration of the zirconium oxide ceramic surface, the preparation method comprises the following steps: cleaning a zirconium oxide matrix and carrying out alkaline activation; forming a polydopamine adhesion bottom layer; branched polyethyleneimine is fixed to enhance the amino density; constructing a network layer through alternate coordination of phytic acid and calcium / strontium ions; strontium doped hydroxyapatite nano particles are immobilized; finally, biomimetic mineralization is carried out in simulated body fluid. According to the method, a micro-nano composite active interface is formed, the biological activity, ion release controllability and osseointegration capability of the material are remarkably improved, and osteoblast response is promoted.

The invention provides a pH-responsive slow-release zinc-phosphorus nano-fertilizer as well as a preparation method and application thereof. The pH-responsive slow-release zinc-phosphorus nano-fertilizer is prepared from the following raw materials: zinc ion-loaded hydroxyapatite and chitosan, the chitosan is at least partially coated on the hydroxyapatite loaded with the zinc ions; the pH-responsive slow-release zinc-phosphorus nano-fertilizer slowly releases zinc and phosphorus elements under the condition that the pH is 4-6. According to the invention, the multi-nutrient nano phosphate fertilizer with pH response of chitosan-coated zinc-loaded hydroxyapatite nanoparticles is developed by constructing nano hydroxyapatite as a carrier, and compared with the traditional phosphate fertilizer, the multi-nutrient nano phosphate fertilizer not only can greatly reduce phosphorus loss and negative influence on the environment, but also can be used as a plant modifier to promote the growth of plants.

The invention discloses a self-curing injectable pectin hydrogel and a preparation method and application thereof.Based on 100 parts by weight of the hydrogel, the hydrogel is prepared from, by weight, 30-80 parts of Pickering emulsion, 0.5-5 parts of pectin and the balance water, based on 100 parts by weight of the Pickering emulsion, 0.5-5 parts of the pectin and the balance water, and based on 100 parts by weight of the Pickering emulsion, 0.5-5 parts of the pectin are added, the Pickering emulsion contains 2 to 20 parts by weight of pluronic F127 and 0.1 to 5 parts by weight of hydroxyapatite, and on the basis of 100 unit volume of the Pickering emulsion, the Pickering emulsion contains 20 to 70 unit volume of ethyl oleate. According to the hydrogel, hydroxyapatite nanoparticles are used as a hydrogel cross-linking agent and also used as a stabilizer for stabilizing a Pickering emulsion, and a biodegradable pectin substrate is combined, so that the effects of promoting bone repair and preventing tumor recurrence are achieved. The system can effectively promote apoptosis of osteosarcoma cells, inhibit migration of the osteosarcoma cells and regulate and control the cycle of the osteosarcoma cells in vitro, and shows a remarkable anti-osteosarcoma effect and an osteogenesis promotion effect in vivo.

The invention relates to a preparation method and application of a human collagen-based multifunctional composite material. Main raw materials of the composite material comprise four-arm polyethylene glycol-sulfydryl, alkenylated collagen, hyaluronic acid and magnesium-doped hydroxyapatite nanoparticles, and finally a multilayer sphere spatial configuration product is formed. The four-arm polyethylene glycol-sulfydryl is a star-shaped polymer, the center of the star-shaped polymer is of a four-arm structure connected by ethyoxyl, and the tail end of each arm is provided with sulfydryl. Through click chemical reaction of sulfydryl and alkenyl, the four-arm polyethylene glycol-sulfydryl serving as an internal framework can react with four pieces of alkenyl collagen at the same time to form four-way three-dimensional cross-linking, so that network compactness is achieved, and the tensile strength of the material can be greatly improved.

The present application belongs to the technical field of hydrogel preparation, and relates to a sandwich structure hydrogel cartilage replacement material and a preparation method thereof. The present application uses grape seed protein, hyaluronic acid and other biocompatible polymers as raw materials, and prepares the cartilage replacement hydrogel material by adopting a biomimetic concept. The obtained hydrogel has a sandwich structure of a drag reduction layer, a strong and tough layer and a mineralization layer. The surface of the drag reduction layer is introduced with a hydrophobic layer and phospholipid molecules, so as to endow the hydrogel with excellent friction performance and long-term wear resistance. In the strong and tough layer, a variety of non-covalent bonds are synergistically crosslinked, so that the obtained hydrogel has very high compressive strength. The mineralization layer contains hydroxyapatite nanoparticles, so that the obtained hydrogel has excellent bone induction potential. The sandwich structure hydrogel of the present application has a water content of 65%, a compressive strength of 71 MPa, and a friction coefficient of about 0.01. After 50,000 friction cycles, the friction coefficient remains unchanged, and the cell viability is as high as 100%, thereby providing strength and long-lasting durability guarantee for the material implantation into a living body.

The application discloses a preparation and application method of a probiotic-loaded electrospun film composition for treating periodontitis, and comprises the following steps: S1, first, an electrospun film (PLGA-nHA) doped with hydroxyapatite nanoparticles (nHA) and polylactic acid (PLGA) is prepared through electrospinning; S2, Lactobacillus plantarum DP189 is cultured; and S3, a preparation of the probiotic-loaded electrospun film. The probiotic-loaded electrospun film composition can improve the symptoms of periodontal tissues of rats, such as reducing inflammatory response, reducing the content of inflammatory factor TNF-alpha, increasing the content of bone saliva protein BSP, and improving the absorption degree of alveolar bone of rats. The probiotic-loaded electrospun film composition can be used for preparing a biological preparation for treating periodontitis, and can also be applied to treating other various diseases in the oral cavity, and has a very wide application prospect.

The embodiment of the invention discloses a nanoparticle composition and application thereof. The nanoparticle composition comprises a first composition, a second composition or a third composition, the first composition comprises nano silicon dioxide and a target modification material; the second composition comprises hydroxyapatite nanoparticles and a target modification material or comprises strontium-doped hydroxyapatite nanoparticles and a target modification material; the third composition comprises nano zinc oxide and a target modification material; the target modification material is a homopolymer or a target copolymer, the homopolymer is polyproline, the polyproline is obtained by polymerizing L-proline-N-carboxyl anhydride, and the concentration range of the L-proline-N-carboxyl anhydride during polymerization is 10 mg / mL to 200 mg / mL. According to the embodiment, the biocompatibility of the material can be improved, the biological functions of improving the sterilization effect and improving the artificial bone repair effect are given to the material, the cytotoxicity is reduced, and the stability of the modified nanoparticles can be improved.