107 results about "Hydroxyapatite nanoparticles" patented technology

The invention discloses a preparation method of a poly (lactic acid-glycolic acid)/hydroxyapatite nanofiber compound bracket for bone repair, which comprises the following steps: adding hydroxyapatite nanoparticles into a mixed solvent of tetrahydrofuran and dimethylformamide, and then, adding poly (lactic acid-glycolic acid) to obtain a mixed solution of the hydroxyapatite and the poly (lactic acid-glycolic acid); carrying out electrostatic spinning on the mixed solution; and obtaining the poly (lactic acid-glycolic acid)/hydroxyapatite nanofiber compound bracket for bone repair. The invention has simple and convenient preparation method and wide material sources, simulates the composition and the structure of natural bones of human bodies, introduces the bioactive ceramic material hydroxyapatite into a degradable poly (lactic acid-glycolic acid) substrate to form a compound nanofiber bracket, has the advantages of good biocompatibility, excellent comprehensive performance, convenient use and the like, can effectively promote the adhesion, growth and function expression of bone cells and meet the biological requirements of the bone tissue engineering.

The invention discloses a magnetic composite material and an application thereof in regeneration and repair of bone tissues, as well as a method for preparing the magnetic composite material and a product prepared by the magnetic composite material, wherein the magnetic composite material comprises the following components: a) 10-50g of polymer material calculated by 100ml of organic solvent, wherein the polymer material is selected from polylactic acid, polyglycolic acid, lactic acid-glycolic acid copolymer, polycaprolactone, polyamide or poly-hydroxybutyric acid; b) 10g of hydroxyapatite nano-particles calculated by 100ml of the organic solvent; and c) 2.5g of gamma-Fe2O3 nano-particles calculated by 100ml of organic solvent, wherein the organic solvent is selected from tetrahydrofuran (THF), dimethylformamide (DMF), dimethylacetamide (DMAc), chloroform or dioxane.

The invention relates to a preparation method of nano-hydroxyapatite/sodium alginate composite material. The obtained material can be used for sewage treatment and used for adsorbing and removing heavy metal ions. The method comprises the following steps: quickly pouring a diammonium hydrogen phosphate aqueous solution in the aqueous solution of calcium nitrate, uniformly stirring and mixing, centrifuging to obtain a precipitate, washing the precipitate by using deionized water, re-dispersing the washed precipitate to the deionized water; adding a stabilizer in the obtained suspension, ultrasonically dispersing to obtain stable hydroxyapatite suspension; controlling the mass ratio of hydroxyapatite to sodium alginate, adding sodium alginate in the hydroxyapatite suspension; continuously heating and stirring, controlling the reaction time, evaporating water, carrying out freeze-drying to obtain the product. The preparation method has the advantages that the preparation process is simple and controllable, the raw material resource is wide, the cost is low, environment is protected, the pollution does not exist, the uniform compounding of the hydroxyapatite nano-particles and sodium alginate is realized, and the uniform dispersion and the non-reunion of the hydroxyapatite nano-particles are guaranteed.

The invention relates to mesoporous hydroxyapatite nonoparticles prepared by a microwave-ultrasonic method, and an application thereof. Inventors of the invention are engaged in developing novel methods for synthesizing mesoporous hydroxyapatite (MHAPN), and provide technical improvements for synthesizing the MHAPN by employing the microwave-ultrasonic method. In the method provided by the invention, no emulsifying agent or structure-directing agent is needed when the MHAPN is synthesized; and the synthesized MHAPN has good dispersibility, relatively good controlled-release effect for medicine and significant antineoplastic activity.

The invention discloses magnetic composite hydroxyapatite nanoparticles. The magnetic composite hydroxyapatite nanoparticles are composed of Fe3O4 nanoparticles and hydroxyapatite nanoparticles, wherein the molar ratio of the Fe3O4 to the hydroxyapatite is (1-10): 1; the magnetic composite hydroxyapatite nanoparticles are spherical aggregate or agglomerate in which the Fe3O4 nanoparticles and the hydroxyapatite nanoparticles are distributed evenly and alternately. The preparation method of the magnetic composite hydroxyapatite nanoparticles is simple; the nanoparticles are capable of efficiently removing heavy metal ions in sewage, and in the later treatment period, quick magnetic separation can be realized; the nanoparticles have the adsorbing capacity of 440mg/g to lead ions, the adsorbing capacity of 238mg/g to cadmium ions, the adsorbing capacity of 139mg/g to zinc ions and the adsorbing capacity of 135mg/g to copper ions in the sewage; the magnetic composite hydroxyapatite nanoparticles have a rapid magnetic response characteristic, and are capable of realizing rapid solid-liquid separation with an external magnetic field, and the separation process is high in separation efficiency, and simple and efficient to operate; and besides, the nanoparticles are not residual in water and do not cause secondary pollution to the water and the environment.

The invention discloses a method for preparing polycaprolacton-chitosan/hydroxyapatite nanoparticles porous composite scaffold. The method comprises mixing polycaprolactone with glacial acetic acid to obtain a mixed solution, adding chitosan powder, pouring into NaOH solution, solidifying and oven-drying to obtain polycaprolactone-chitosan porous material; mixing hydroxyapatite nanoparticles to polyvinyl alcohol aqueous solution to obtain a slurry; placing the polycaprolactone-chitosan porous material in a centrifuge tube, and performing grout-filling with the slurry for 3-5 times. The produced composite scaffold has the advantages of good biological and mechanical properties and controllable porosity, and can be widely used as tissue-engineering scaffold for repairing various bone defects.

A nano-class biomedical hydroxyapatite-silicon rubber material is prepared through dissolving silane coupling agent in water-alcohol solution, regulating pH value to 3.5-5.5, mixing with hydroxyapatite nanoparticles while stirring for surface modifying, washing the solid with deionized water, proportionally mixing it with raw silicon rubber, drying, and thermal sulfurizing.

The invention discloses a method for preparing a mesoporous hydroxyapatite nanometer particle with high specific surface area by virtue of a template method. The method comprises the following steps: firstly, mixing calcium pantothenate, F123 and a certain amount of sym-trimethylbenzene to prepare an emulsion and then dropwise adding a phosphate solution with a certain pH value into the emulsion, heating in a water bath, carrying out reflux reaction and filtering to obtain a precipitate and finally calcining the precipitate in a muffle furnace to remove a template to obtain the hydroxyapatite nanometer particle with a mesoporous structure. The mesoporous hydroxyapatite prepared by the method disclosed by the invention has the characteristics of high specific surface area, large drug loading amount and uniform pore size distribution and is distributed in a spherical nanometer state (the size is less than 100nm). The mesoporous hydroxyapatite serving as a drug carrier can well penetrate through blood vessels and cell walls to reach diseased cells. The entire preparation process is simple and the mesoporous hydroxyapatite can be produced in a large scale.

The present invention discloses an aggregation induced luminescence nanofluorescent probe which coats a chitosan fluorescent molecule with the characteristic of aggregation induced luminescence on the surface of a hydroxyl apatite nanoparticle, and the structural formula of the chitosan fluorescent molecule is shown in a formula (1). The aggregation induced luminescence nanofluorescent probe is prepared by a chemical co-precipitation process. The size of the aggregation induced luminescence nanofluorescent probe is about 110 nm, the dispersion is stable, positive changes are formed on the surface thereof, and the aggregation induced luminescence nanofluorescent probe has the feature of the aggregation induced luminescence. Compared with a traditional fluorescent probe, the aggregation induced luminescence nanofluorescent probe has the advantages that the sensitivity is high, the light stability is good, and quenching does not exist during high concentration and a fluorescence spectrum is not drifted, so that the aggregation induced luminescence nanofluorescent probe can be applied to the fields of bioimaging, fluorescent tracing detection and the like. The aggregation induced luminescence nanofluorescent probe is shown in the figure below.

The invention discloses a magnetic and luminous bifunctional hydroxyapatite nanoparticle in a core-shell structure and belongs to the technical field of nano functional material preparation. According to the method, pure hydroxyapatite continues to grow on the surface of a magnetic hydroxyapatite nanoparticle by controlling a coprecipitation condition to realize hydroxyapatite thin layer coating of the magnetic particle, so that dissolving of magnetic ions (such as Fe<3+>) quenching luminescence of a rare earth ion is inhibited. Rare earth doped hydroxyapatite continues to grow on the surface of the magnetic hydroxyapatite nanoparticle with a hydroxyapatite coating layer, so that the magnetic and luminous hydroxyapatite nanoparticle in the core-shell structure is obtained. The core-shell type magnetic and luminous hydroxyapatite nanoparticle has good magnetic property, luminescence property and biocompatibility, further has target positioning and mark tracking functions, and has good application prospects in the field of biomedicine such as substance detection inside and outside a cell, nucleic acid hybridization and sequencing, early disease diagnosis, transport of gene drugs, and magnetic heat treatment.

The invention provides a hydroxyapatite/chitosan-silk fibroin nanocomposite and a preparation method thereof. The preparation method comprises the technological processes of coprecipitation reaction of a hydroxyapatite precursor in a wet state with chitosan and silk fibroin, separation of a generated precipitate from a liquid phase system, drying of the separated precipitate, subsequent processing and molding and the like. The preparation method has the advantages that hydroxyapatite nanoparticles can be ensured to uniformly disperse in a chitosan-silk fibroin composite organic matrix, so that the formed composite material not only has favorable biocompatibility and bioactivity, but also has sufficient mechanical strength and toughness; chitosan and silk fibroin are abundant in sources and low in prices, the preparation process is carried out under the conditions of normal temperature and normal pressure, thus, the operation is convenient and the cost is low. The hydroxyapatite/chitosan-silk fibroin nanocomposite prepared by the invention is an ideal new-generation substitute material for bone repair.

An application of the acicular hydroxyapatite nanoparticles in preparing toothpaste for repairing and strengthening tooth and preventing decayed tooth is disclosed.

The invention relates to a hydroxyapatite nanoparticle with antitumor activity, a preparation method and application thereof. The invention discloses the near-spherical nano-hydroxyapatite with a particle size of 10-100nm, amorphous state and uniform particle distribution. The nanoparticle has very excellent antitumor activity and a tumor cell inactivation rate up to about 80%. The hydroxyapatite nanoparticle is prepared by a liquid phase precipitation method. The invention also discloses the use of the nanoparticle, and a pharmaceutical composition and a medicine box containing the nanoparticle.

The invention belongs to the technical field of biomedical materials, and discloses a shape memory-type bioactive nano particle/biodegradable polyester composite multi-scale pore biological scaffold and a preparation method thereof. The method of the invention comprises the steps of: adding biodegradable shape memory polyester, hydroxyapatite nano particles and a rheology modifier to an organic solvent, and performing ultrasonic effect to obtain an oil phase; adding water to the oil phase, and performing emulsification to form water-in-oil high-internal phase emulsion; and taking the high-internal phase emulsion as an ink, adopting extrusion-type 3D printing to obtain a three-dimensional porous emulsion scaffold, and evaporating the solvent to obtain the biological scaffold. The scaffold contains a multi-scale pore structure, in which controllable micropores and macropores coexist. The porosity is higher than 85%, and the pore structures are connected to one another, so that the scaffold has excellent biocompatibility, biodegradability, biological activity, shape memory effect, and drug growth/factor loading and release, and can be applied in the field of drug controlled release and the field of tissue engineering scaffolds.

The invention discloses a method for preparing surface molecular imprinting microspheres through polymerization of Pickering emulsion based on hydrophobic hydroxylapatite nanometer stable particles and application. The method for preparing the surface molecular imprinting microspheres comprises the steps that the hydrophobic hydroxylapatite nanometer particles are used as stable particles, protein is used as template molecules, polymeric microspheres are prepared through self-assembly of dopamine monomers in an aqueous phase, the template molecules are further removed through eluent, and the surface molecular imprinting microspheres can be obtained. Print sites are arranged on the surfaces of the surface molecular imprinting microspheres, the surface molecular imprinting microspheres can be in full contact with target molecules in a solution, efficient specific adsorption on the target molecules can be achieved, and the surface molecular imprinting microspheres can be widely applied to specific adsorption and separation of protein in a biosystem. According to the method, preparation is easy to grasp, preparation is easy and convenient, cost is low, and large-scale industrial production is greatly facilitated.

The invention discloses a preparation method of green fluorescently labeled terbium/strontium co-doped hydroxyapatite nanoparticles with high luminous intensity. First, take Tb ₄ o ₇ Add excess concentrated nitric acid, heat in a water bath, and evaporate to form Tb(NO ₃ ) ₃ crystal, and then prepare terbium nitrate solution; secondly, calcium nitrate, strontium nitrate, diammonium hydrogen phosphate and sodium hydroxide are dissolved in deionized water to form a solution, and the terbium nitrate solution in (1) is mixed with calcium nitrate solution and strontium nitrate solution Evenly, add the diammonium hydrogen phosphate solution dropwise, adjust the pH value to 14 with sodium hydroxide solution after fully mixing, and fully stir to obtain solution A after the pH value is stable; finally, leave solution A to stand for aging, and then After washing, suction filtration, drying and grinding, green fluorescent-labeled Tb with high luminescence intensity can be obtained ³⁺ /Sr ²⁺ Co-doped with hydroxyapatite nanoparticles. The material prepared by the present invention compares Tb ³⁺ Doped with hydroxyapatite, the fluorescence intensity is higher, the color is more obvious, and the fluorescence lifetime is longer.

The invention discloses a preparation method of spherical nano hydroxyapatite particles. The preparation method comprises the following steps: (1) measuring a CQDs (carbon quantum dots) solution in a conical flask, regulating the pH value of the solution up to 10, and uniformly stirring at room temperature; (2) dropwisely adding a CaCl2 solution, and uniformly stirring; (3) dropwisely adding a Na2HPO4.12H2O solution, and standing at room temperature; (4) performing centrifugal separation on the prepared product, discarding the supernate, washing the underlayer product with distilled water and anhydrous ethanol respectively, then taking out the product, and drying in a drying oven to obtain khaki powder; and (5) calcining the powder in a muffle furnace, thereby removing carbon quantum dots to obtain white powder, namely HAP (hydroxyapatite) nanoparticles. According to the invention, the spherical hydroxyapatite nanoparticles are prepared at room temperature by using the carbon quantum dots as effective nucleation inducer through a chemical precipitation method. The hydroxyapatite prepared by the invention is quasi-spherical, and has the advantages of favorable dispersity, particle uniformity and high crystallinity. Besides, the preparation method has the advantages of simplicity, mild and controllable conditions and low cost.

The invention discloses a surface-enhanced Raman spectroscopy (SERS) detection method based on adsorption of proteins by hydroxyapatite nanoparticles. The SERS detection method comprises the steps of:carrying out mixed incubation on hydroxyapatite nanoparticles and a biological sample and absorbing proteins in the sample, adding acid into the mixture to adjust a pH value so as to enable the proteins to fall off from the surfaces of the hydroxyapatite nanoparticles, extracting supernate containing the fallen proteins, uniformly mixing the supernate with an SERS nano active material, detectingto obtain a biological sample protein surface enhanced Raman spectrum by utilizing an SERS technology, and establishing a biological sample protein surface enhanced Raman spectrum database; and acquiring characteristic spectral peaks of surface enhanced Raman spectra corresponding to different biological sample proteins through post-processing and analysis of the data. The SERS detection method has the advantages of quickness, simplicity in operation, low cost and the like, and can be used for effectively detecting the surface enhanced Raman spectra of the proteins in different biological samples.

The invention belongs to the field of nano-biological materials, and discloses a method for preparing small-sized nano-hydroxyapatite by using carbon dots (CDs) as a template. The method comprises thefollowing steps: weighing solids H2N(CH2)10COOH and NaOH and dissolving in deionized water, then adding a citric acid solution and stirring for reacting, collecting white precipitate, drying, grinding, and then carrying out a thermal oxidization reaction at 300 DEG C to obtain the CDs; adjusting the pH of a Ca(NO3)2 solution to 10.5 with an alkaline solution, then dropwise adding the CDs to carryout a complexation precipitation reaction, dropwise adding an Na3PO4 solution, carrying out a hydrothermal reaction at 140 to 180 DEG C, and washing and drying a product to obtain the small-sized nano-hydroxyapatite. Hydroxyapatite nanoparticles prepared by the method are shaped like short rods, are smaller in size than particles without the CDs, can be reduced in particle size through the increase of the CD content, and have a potential application prospect in the field of nanomedicine.

The present invention discloses a CEA concentration detection probe, which comprises a CEA aptamer and hydroxyapatite nanoparticles, wherein the CEA aptamer and the hydroxyapatite nanoparticles are integrally compounded through a cross-linking agent, the CEA aptamer contains the nucleotide sequence 5'-ATACCAGCTTATTCAATT-3' and has the 5'-NH2 modified 5' terminal, and the surface of the hydroxyapatite nanoparticles is coated with a chitosan film. According to the present inventin, the hydroxyapatite and the aptamer contained in the CEA concentration detection probe can respectively react with sodium molybdate to produce electrochemical signals so as to achieve the double signal amplification and improve the detection sensitivity; and with the application f the CEA concentration detection probe in the preparation of the biosensor for CEA concentration detection, the advantages of high detection sensitivity, low detection limit and wide detection range are provided.

The invention discloses a hydroxyapatite-based electrochemical probe and a construction method thereof, and a method for determining the activity and the inhibition property of BACE1. According to the present invention, an A[beta] antibody and alkaline phosphatase are co-modified on a hydroxyapatite matrix to form the hydroxyapatite-based electrochemical probe; the preparation method comprises: sequentially placing hydroxyapatite nanoparticles in a polyethylenimine solution and carrying out a reaction, placing in a glutaraldehyde solution and carrying out a reaction, and placing in a solution containing an A[beta] antibody and alkaline phosphatase and carrying out a reaction so as to obtain the probe; and the method for determining the activity and the inhibition property of BACE1 by using the hydroxyapatite-based electrochemical probe has advantages of simpleness, rapidness, high sensitivity and wide detection range, and can be widely promoted and applied.