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CEA concentration detection probe, preparation method and applications thereof, and CEA concentration detection biosensor

A biosensor, concentration detection technology, applied in biochemical equipment and methods, DNA/RNA fragments, instruments, etc., can solve the problems of high detection limit, low sensitivity detection, narrow detection range, etc., achieve low detection limit, selectivity Good, wide detection range effect

Inactive Publication Date: 2017-07-14
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the existing electrochemical detection methods mainly use single-signal detection, which still has problems such as low sensitivity detection, high detection limit, and narrow detection range.

Method used

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  • CEA concentration detection probe, preparation method and applications thereof, and CEA concentration detection biosensor
  • CEA concentration detection probe, preparation method and applications thereof, and CEA concentration detection biosensor
  • CEA concentration detection probe, preparation method and applications thereof, and CEA concentration detection biosensor

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Embodiment 1

[0043] The preparation method of the CEA concentration detection probe of this embodiment includes: dispersing 1 mg of hydroxyapatite nanoparticles in 0.1% chitosan solution, and reacting at room temperature for 2 hours; after centrifugal washing, the obtained hydroxyapatite The nanoparticles are dispersed in 0.25% glutaraldehyde solution and reacted for 1 hour; after centrifugation and washing, the hydroxyapatite nanoparticles are reacted with a 10 μM aptamer solution for 1 hour; after centrifugation and washing to remove unreacted aptamers, the present invention is obtained. CEA concentration detection probe. in, figure 2 It is the TEM picture of the raw material of hydroxyapatite nanoparticles. image 3 It is a schematic diagram of the microstructure of the CEA concentration detection probe, and the CEA concentration detection probe includes CEA aptamer 1 and hydroxyapatite nanoparticles 2.

[0044] The CEA concentration detection sensor of this embodiment includes a CEA...

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Abstract

The present invention discloses a CEA concentration detection probe, which comprises a CEA aptamer and hydroxyapatite nanoparticles, wherein the CEA aptamer and the hydroxyapatite nanoparticles are integrally compounded through a cross-linking agent, the CEA aptamer contains the nucleotide sequence 5'-ATACCAGCTTATTCAATT-3' and has the 5'-NH2 modified 5' terminal, and the surface of the hydroxyapatite nanoparticles is coated with a chitosan film. According to the present inventin, the hydroxyapatite and the aptamer contained in the CEA concentration detection probe can respectively react with sodium molybdate to produce electrochemical signals so as to achieve the double signal amplification and improve the detection sensitivity; and with the application f the CEA concentration detection probe in the preparation of the biosensor for CEA concentration detection, the advantages of high detection sensitivity, low detection limit and wide detection range are provided.

Description

technical field [0001] The invention belongs to the technical field of biosensors, in particular to the field of carcinoembryonic antigen (CEA) concentration detection, in particular to a CEA concentration detection probe, a preparation method and application thereof, a CEA concentration detection biosensor and a preparation method thereof. Background technique [0002] Carcinoembryonic antigen (CEA) is a broad-spectrum tumor marker, which can reflect the existence of a variety of tumors, and is a good tool for judging the curative effect, disease development, monitoring and prognosis of colorectal cancer, breast cancer and lung cancer. tumor markers. 97% of healthy adults have serum CEA concentrations below 2.5 μg / L, and 45% to 80% of patients with primary colon cancer have elevated serum CEA concentrations. In addition to primary colon cancer, 50% to 70% of patients with pancreatic cancer, cholangiocarcinoma, gastric cancer, esophageal cancer, adenocarcinoma, lung cancer,...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/36G01N27/30G01N27/48G01N33/574C12N15/115
CPCG01N27/3277C12N15/115C12N2310/16C12N2310/3535G01N27/308G01N27/3278G01N27/36G01N27/48G01N33/57473
Inventor 阳明辉李亭
Owner CENT SOUTH UNIV
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