980 results about "Signal amplification" patented technology

Provided herein are methods of determining a location or presence of a target analyte in a biological sample by detecting oligonucleotide-conjugated binding agents.

The invention belongs to the field of immunological detection, and provides a double-antibody sandwich ELISA kit for detecting the content of human beta-NGF. The enzyme-linked immunosorbent assay kit constructed by the invention is based on a double-antibody sandwich method, and is combined with a biotin-streptavidin signal amplification system and an enzyme developing amplification system, so that the detection sensitivity and specificity are greatly improved, and low-content samples can be accurately and quickly detected. The kit shows excellent stability and specificity and can be used for quantitatively detecting the content of beta-NGF in samples such as human serum, cell supernatant or tissue homogenate, so that an objective detection basis is provided for diagnosis, curative effect evaluation and pathogenesis research of related diseases.

The invention provides a low-noise amplification circuit, a radio frequency front-end module and an electronic terminal.The low-noise amplification circuit comprises an input matching adjustment circuit, a common-source amplification circuit, a first inductor, an inter-stage feedback circuit, a common-gate amplification circuit, an input and output feedback circuit, an output matching adjustment circuit and an output attenuation circuit; the common-source amplification circuit comprises at least two first signal amplification units; each first signal amplification unit comprises a first field effect transistor; the common-gate amplification circuit comprises at least two second signal amplification units; and each second signal amplification unit comprises a second field effect transistor. According to the low-noise amplification circuit, transconductance nonlinearity of devices under different bias signals can be mutually counteracted, so that the linearity fluctuation of the low-noise amplification circuit at different temperatures is optimized, and the linearity of the low-noise amplification circuit is kept basically not to change along with the temperature change.

The invention relates to a beam feedback system based on radio frequency direct acquisition, which comprises a beam position detector, a sum and difference device, a front-end processing module, a feedback processor and an amplification excitation module which are connected in sequence, the sum-difference device is used for determining a horizontal position signal, a vertical position signal and a longitudinal position signal of the beam bunch according to the position signal of the beam bunch, the front-end processing module is used for pre-processing the position signals of the beam bunch, and the feedback processor is used for sampling the pre-processed position signals of the beam bunch; the feedback module is used for receiving a beam bunch and generating a horizontal feedback signal, a vertical feedback signal and a longitudinal feedback signal of the beam bunch, and the amplification excitation module is used for amplifying the feedback signals of the beam bunch and acting the amplified feedback signals of the beam bunch on the beam bunch, so that the instability of the beam is inhibited.

The utility model provides a passive infrared human body detection sensor. The passive infrared human body detection sensor comprises a shell which plays a role in protection and fixation; the Fresnel lens is assembled at the opening end of the shell and is used for converging external infrared radiation and segmenting a sensor detection area; the MEMS galvanometer is assembled at the focus of the Fresnel lens and is fixed on the shell through a bracket structure; the control circuit board is fixed on the mounting supporting column of the shell through screws, a pyroelectric sensing unit, an AD conversion circuit and a microcontroller MCU unit are assembled on the control circuit board, and the pyroelectric sensing unit comprises two pyroelectric sensing units, an induction window covering the pyroelectric sensing units and a signal amplification and filter circuit; a sensing window of the pyroelectric sensing unit faces the MEMS galvanometer, and the normal direction of the pyroelectric sensing unit is perpendicular to the central axis of the Fresnel lens. According to the utility model, static human bodies in a detection area can be effectively detected, environmental noise interference can be effectively overcome, and high detection sensitivity is also provided for human bodies moving in the radial direction and in the horizontal direction.

Systems, devices, and methods are provided for pulsed strain gauge topologies. A weight sensor assembly may comprise one or more load cells for receiving excitation pulses during a first state period of a duty cycle, sample and hold (S / H) circuitry connected to the one or more load cells that holds analog measurement signals from the one or more load cells, first filtering circuitry connected to the S / H circuitry for processing the analog measurement signals and producing a DC level signal, an amplification system connected to the filtering circuitry, for amplifying the DC level signal, second filtering circuitry connected to the amplification system for filtering power line noise, and analog-to-digital (ADC) circuitry connected to the second filtering circuitry. Examples of load cells described herein include full-bride load cells (FBLCs) and half-bridge load cells (HBLCs).

The utility model provides a sampling circuit, an analog circuit and electronic equipment, and the sampling circuit comprises an input processing unit which is connected with the output end of a target circuit, and is used for collecting the common-mode output voltage of the target circuit, and carrying out the sampling input processing of the common-mode output voltage, and obtaining a first operational amplifier in-phase sampling signal and a first operational amplifier anti-phase sampling signal; the signal amplification unit is connected with the input processing unit and is used for respectively amplifying the first operational amplifier in-phase sampling signal and the first operational amplifier anti-phase sampling signal to obtain a second operational amplifier in-phase sampling signal and a second operational amplifier anti-phase sampling signal; and the signal stabilization unit is connected with the signal amplification unit and the control circuit, and is used for keeping the second operational amplifier in-phase sampling signal and the second operational amplifier anti-phase sampling signal, generating a feedback signal according to the second operational amplifier in-phase sampling signal and the second operational amplifier anti-phase sampling signal, and outputting the feedback signal to the control circuit. In this way, the input impedance and the sampling precision are improved.

The invention discloses construction and application of a confinement enhanced photoelectrochemistry-electrochemiluminescence dual-mode biosensor, and relates to the technical field of biosensing and analytical chemistry. The preparation method of the biosensor comprises the following steps: taking a CdS (at) SiO2 (at) NaYF4: Yb / Tm nano composite material as a substrate to obtain a confinement enhanced photoelectrochemistry (PEC) and electrochemiluminescence (ECL) sensing interface; in combination with a DNA wheel nanostructure triggered by target acetamiprid, signal amplification is realized; and synchronous dual-mode quenching detection of PEC and ECL signals is realized by virtue of peroxidase-like catalytic precipitation and wide-spectrum absorption characteristics of the PtPd-CoSnO3 nanocube. The biosensor prepared by the invention has the advantages of high sensitivity, strong reliability, good selectivity and the like, can be applied to accurate detection of acetamiprid in samples such as agricultural products and the like, and has important application value in the aspects of environmental monitoring and food safety control.

The application relates to a DNAzyme-based gene chip biosensor and application thereof, and belongs to the technical field of electrochemical sensors. In order to solve the technical problems of low specificity and accuracy, long processing time and high cost in the single nucleotide polymorphism detection method in the prior art, a DNAzyme-based gene chip biosensor is provided, which comprises a DNAzyme molecular probe SNP recognition system and a solid-liquid phase mixed catalytic network system. The application provides a proteinase-free and space-oriented biosensing strategy, utilizes a molecular switch based on a DNAzyme probe to realize accurate SNP recognition, utilizes a space steric hindrance parallel amplification system to enhance signal amplification and simultaneously inhibit background noise, so that the detection performance is remarkably improved; and the gene chip biosensor can be applied to genotyping and trait selection in crop improvement and other applications.

An alternating-current electromagnetic field stress measurement system, comprising: an excitation signal generation module, an alternating-current electromagnetic field stress measurement stress probe, a signal amplification circuit, an A / D conversion circuit and a processor, wherein the excitation signal generation module is used for loading a sinusoidal alternating-current signal to an excitation coil of the alternating-current electromagnetic field stress measurement stress probe, such that a periodically changing electric field is generated on a measured magnetic object; the alternating-current electromagnetic field stress measurement stress probe is used for sequentially routing a stress characterization signal, which is detected from the measured magnetic object, through the signal amplification circuit and the A / D conversion circuit, and then sending the stress characterization signal to the processor; and the processor is used for processing the received stress characterization signal by using an optimized J-A model in which an implicit equation originally characterizing a change in the magnetization intensity or magnetic induction intensity along with stress is made explicit, so as to calculate the stress of the measured magnetic object. The alternating-current electromagnetic field stress measurement system can monitor the stress increase trend in real time, and notice potential dangers in time. Further comprised is an alternating-current electromagnetic field stress measurement method and a computer program for executing the alternating-current electromagnetic field stress measurement method.

The invention relates to a multi-machine calibration method and system for a Raman spectrometer, and a storage medium, and the method comprises the steps: outputting an adjustable substrate voltage, and setting an initial signal amplification gain; controlling a CCD sensor to collect first Raman spectrum data of a standard sample with a known standard wave number; establishing a mapping model from a pixel domain to a wave number domain based on a plurality of known standard wave numbers and second pixel point positions of the characteristic peaks corresponding to the known standard wave numbers in the first Raman spectrum data to obtain calibration parameters of the Raman spectrometer; verifying the accuracy of the mapping model by verifying the characteristic peak, and storing calibration parameters after the verification is passed; controlling the plurality of Raman spectrometers to respectively detect the same sample to be detected, acquiring second Raman spectrum data of the sample to be detected, extracting a third pixel point position corresponding to a characteristic peak of the sample to be detected, calling the calibration parameter, and converting the calibration parameter into an actually measured wave number; and feeding back and adjusting the signal amplification gain of each instrument based on a signal intensity measurement value corresponding to the second Raman spectrum data by each Raman spectrometer.

The invention relates to the technical field of detection kits, in particular to a blood coagulation reaction linked lupus anticoagulant fluorescence detection kit and application thereof, the kit contains a screening-confirmation integrated reagent, a calibration product, a quality control product and an anticoagulation interference inhibitor; the integrated reagent is divided into a reagent A and a reagent B, the reagent A contains a buffer solution, recombinant snake venom thrombin, composite phospholipid, a fluorescent substrate and other components, and the reagent B contains prothrombin, calcium ions, high-concentration composite phospholipid and other components; the composite phospholipid system is formed according to a specific proportion and modifies beta2-glycoprotein I, the fluorescence enhancement-stabilization system is formed by matching a Schiff base compound with a stabilizer, the calibrator is a series of lupus anticoagulant standard substances, and the quality control substance comprises simulants with different concentrations and negative control. The anti-interference capability is high, and the influence of anti-coagulant drugs and enzyme source fluctuation is reduced; the signal amplification effect is obvious, the detection sensitivity is high and the reagent stability is good; the operation is simple and convenient, screening and confirmation are integrally completed, and a conventional fluorescence detector is adapted.

Certain aspects of the present disclosure generally relate to electronic circuits and, more particularly, to techniques and apparatus for signal amplification. One example apparatus generally includes: a current sensor configured to sense a current associated with an amplifier; and a voltage sensor configured to sense a voltage associated with the amplifier. The apparatus may also include a logic circuit configured to: detect a first over-current condition associated with the amplifier based on the current; detect a first over-voltage condition associated with the amplifier based on the voltage; and adjust an amplification gain to yield a first adjusted gain level for an input signal based on detection of the first over-current condition and the first over-voltage condition, wherein the amplifier is configured to amplify the input signal based on the first adjusted gain level.

The invention discloses a composition, a detection method and a kit for SpaceAS variable splicing space in-situ detection of nucleic acid. The composition comprises a pi-type probe, a U-type probe, an O-type probe, an S-type probe and a signal probe, and the O-type probe adopts an in-vitro cyclization strategy, so that the problem of low hybridization efficiency caused by dependence on enzymatic cyclization reaction in a traditional hybridization method is effectively solved; meanwhile, an efficient probe hybridization system, a high-sensitivity signal amplification mechanism and a multi-round iterative imaging process are combined, so that the detection sensitivity and specificity are remarkably improved. The detection method has the advantages of high efficiency, high sensitivity, high specificity, good repeatability, strong expandability and the like through the synergistic effect of five-step single-stranded DNA probe hybridization reaction and one-step DNA amplification reaction, and can be widely applied to space in-situ detection and analysis of variable splicing events.

The application provides a preparation method of a functionalized phage probe and application of the functionalized phage probe in detection of escherichia coli. The functionalized phage is a recombinant phage (M13KO7@SaBP) in which streptavidin binding peptide (SaBP) is fused and expressed at the N terminal of the main capsid protein P8 protein of M13K07 phage. In the application, the gene encoding SaBP (MDVEAWLGAR) is inserted into the N terminal of the P8 protein gene of M13K07 phage by site-directed mutagenesis to prepare M13K07@SaBP. The stable signal amplification is realized by using the abundant P8 protein (about 2700) of M13 phage and the super strong binding force of biotin-streptavidin, the interference of food sample matrix is reduced by combining the magnetic separation technology, and a detection method of escherichia coli with high sensitivity and small sample matrix interference is constructed.

The embodiment of the invention provides a receiving front-end amplifier, a signal amplification method and network equipment. The receiving front-end amplifier comprises a current mode logic unit which is used for converting a first single-ended input signal into a differential signal according to a reference voltage locally generated by the receiving front-end amplifier; the negative capacitance unit is used for expanding the bandwidth of the differential signal; and the conversion unit is used for converting the differential signal after bandwidth expansion into a second single-ended signal and outputting the second single-ended signal. According to the embodiment of the invention, based on the current mode logic, negative capacitance and differential-to-single-ended three-stage circuit design, the problems of insufficient bandwidth, reduced signal quality and over-high power consumption during single-ended signal input can be effectively solved, and the effect of enhancing the high-speed signal processing performance is achieved.

The invention provides in-situ forming fluid bionic hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The invention provides an injectable GelSSO / PDA (at) SDF fluid bionic niche, after in-situ photo-crosslinking, preferential and sustained release of PDA (at) SDF NPs initiates early signal amplification, which recruits EPCs and MSCs through an SDF-1 alpha / CXCR4 axis, promotes activation of the angiogenic vascular niche through an AKT signal, and repolarizes macrophages to a regenerated M2 phenotype. Subsequently, the gradual degradation of the bionic niche triggers the stable release of SSO, and the later signal is further amplified to form an osteogenic niche with transcriptional activity, thereby maintaining the MAPK / ERK-mediated MSCs osteogenic differentiation. The fluid bionic niche can form a niche conforming to defects through photopolymerization, the local immune imbalance and endogenous progenitor cell recruitment are corrected in the early stage, then formation of new vessels and lamellar bones is promoted, and finally vascularization regeneration of diabetic skull defects is achieved.

The invention belongs to the technical field of radio frequency identification, and particularly discloses a multi-antenna diversity reception high-frequency RFID signal processing system and method, and the system comprises a multi-antenna array, a multi-channel amplification notch module, a high-frequency-to-intermediate-frequency module, an MRC processing module, an AGC circuit, and a demodulation module which are sequentially connected according to the signal flow direction. Spatial diversity reception is realized through a multi-antenna array, low-noise amplification and self-interference suppression are synchronously completed by the multi-channel amplification trap wave module, amplitude weighting and phase compensation combination of multi-channel intermediate frequency signals are realized by the MRC processing module after frequency spectrum shifting of the high-frequency-to-intermediate frequency module, and the multi-channel high-frequency-to-intermediate-frequency signal is obtained. Power is stabilized through the AGC circuit, signal amplification and analog-to-digital conversion are completed through the demodulation module, and finally pure digital baseband signals are output. Through the technical means, the anti-interference capability and the receiving sensitivity of the high-frequency RFID receiver in a complex environment can be effectively improved, and the requirement for long-distance and high-reliability electronic tag reading is met.

The application discloses a kind of trusted time transfer device and method, it is related to trusted time transfer technical field, including network management system and trusted time transfer equipment;Network management system is used for equipment identity solidification and authentication and equipment operating state real-time monitoring;Trusted time transfer equipment is based on trusted time signal reception relay module, equipment identity authentication pre-solidification mechanism, two-way certificate verification and transmission encryption mechanism, equipment failure stop signal transmission mechanism and the traceability and proof mechanism of time signal, real-time measurement is carried out to trusted time signal, time delay compensation, signal amplification processing is transferred out;The application ensures that itself is not replaced by strict control mechanism;On signal processing, the controllability of time real-time detection and time delay compensation is realized;In addition, it also provides a feasible path for the traceability verification and credibility proof of time signal with time relay function.

The invention relates to the technical field of immunochromatography analysis, and discloses a signal enhancer of a colloidal gold immunochromatography rapid detection card and a preparation method thereof, the signal enhancer comprises a silver ion solution, a thiourea-ascorbic acid synergistic reducing agent, a pH buffer system and a surfactant stabilizing system, the signal amplification is realized by catalyzing and depositing silver nanoparticles on the surface of colloidal gold, the operation is simple and convenient, one-time addition is realized, and the reaction is completed within 5-10 minutes; the sensitivity is improved by 5-10 times, and the specificity is higher than 98%; the stability is excellent, and the storage time is 12 months at 4 DEG C; various colloidal gold rapid detection cards are universally compatible; compared with the prior art, the colloidal gold immunochromatography rapid detection card has the advantages that the cost is controllable, the single detection is increased by 0.3-0.5 yuan, and the colloidal gold immunochromatography rapid detection card is particularly suitable for ultra-sensitive detection of small molecular substances in the field of food safety, for example, the detection limit of malachite green reaches 0.1 microgram per liter, the detection limit of fipronil reaches 0.5 microgram per liter, and the application range of the colloidal gold immunochromatography rapid detection card is obviously expanded.

The invention discloses a miniature front scattering type visibility meter capable of being carried on an unmanned aerial vehicle, which is applied to the field of visibility meters and is characterized in that modulated light rays are irradiated to a reflector I through an emission light path collimating lens cone and then are reflected out of a detection cabin along the direction of an emission channel, and when passing through atmospheric dispersion substances on the lower side of the detection cabin, the light rays are reflected out of the detection cabin along the direction of the emission channel; part of light rays are scattered, enter a receiving chamber through a receiving channel, are reflected by a second reflector, penetrate through a receiving light path collimating lens barrel and irradiate a receiving detector, received electric signals pass through a signal amplification circuit and then are subjected to AD sampling, analog signals are converted into digital signals, the digital signals are sent to a control chip, the digital signals are filtered, and the digital signals are sent to the control chip. The forward scattering visibility meter has the advantages that signal envelope is extracted through transformation, wavelet transformation and noise reduction are performed, extinction coefficients and visibility are finally calculated, the forward scattering visibility meter is miniaturized through special design of a light path system, the use requirements of small size, small wind resistance and light weight are met, and the problem that the visibility meter is difficult to apply to an unmanned aerial vehicle is solved.

The invention belongs to the field of immunodetection kits, and provides a P-Tau231 single-molecule immunodetection kit based on signal amplification and application of the P-Tau231 single-molecule immunodetection kit. The kit comprises a solid-phase carrier, a detection antibody-signal amplification probe conjugate and the like, wherein a total Tau non-phosphorylated region specific first antibody is fixed on the surface of the solid-phase carrier; the detection antibody-signal amplification probe conjugate is used for recognizing P-Tau231 phosphorylated 231 sites; quantitative detection is realized by adopting a sandwich immune sandwich mode and combining with a single-molecule detection platform. The detection limit of the kit reaches fg / mL to ag / mL, and the kit has the advantages of ultrahigh sensitivity, high specificity, simplicity and convenience in operation and low sample demand, can efficiently detect P-Tau231 in cerebrospinal fluid and plasma, and is suitable for preparation of early diagnosis products for Alzheimer's disease.

The invention relates to a normal temperature amplification-CRISPR Cas12a one-pot method detection system and a nucleic acid detection method based on the detection system. The detection system consists of a normal-temperature nucleic acid amplification system (unit I) which focuses on target signal amplification and a CRISPR Cas12a system (unit II) which focuses on target recognition and signal output. By means of a self-driven and deoxyribozyme-mediated reaction equilibrium transfer strategy, the equilibrium in the initial stage of the reaction tends to the unit I, and the equilibrium in the middle and later stages of the reaction tends to the unit II, so that the compatibility of the two units is remarkably improved. By means of the system, high-sensitivity and high-specificity portable one-pot detection of nucleic acid targets can be achieved.

The embodiment of the present application provides a receiving front-end amplifier, a signal amplification method and network equipment. The receiving front-end amplifier comprises: a current mode logic unit, which is used for converting a first single-ended input signal into a differential signal according to a reference voltage generated by the receiving front-end amplifier locally; a negative capacitance unit, which is used for expanding the bandwidth of the differential signal; and a conversion unit, which is used for converting the differential signal with the expanded bandwidth into a second single-ended signal and outputting the second single-ended signal. Through the embodiment of the present application, based on the current mode logic, the negative capacitance and the differential-to-single-ended three-stage circuit design, the problems of insufficient bandwidth, signal quality decline and excessive power consumption when the single-ended signal is input can be solved effectively, and the performance of high-speed signal processing is enhanced.

The invention discloses an immunofluorescence imaging method for driving tyramine signal amplification based on chemiluminescence and application thereof, and relates to the technical field of biomedical imaging. The immunofluorescence imaging method comprises the following steps: carrying out first incubation on a to-be-detected sample and a peroxidase-labeled antibody; performing second incubation on the to-be-detected sample after the first incubation and a tyramine substrate containing a fluorophore under a dark condition; adding a chemiluminescent substrate solution into the to-be-detected sample after the second incubation, and carrying out image acquisition; wherein the antibody can be specifically bound with a target protein; the tyramine substrate can generate active free radicals under the catalysis of peroxidase, so that the active free radicals are covalently cross-linked with tyrosine residues; the chemiluminescent substrate solution comprises hydrogen peroxide and luminol. The immunofluorescence imaging method improves imaging signal and fluorescence stability, effectively reduces background interference and phototoxicity, is suitable for long-time stable fluorescence immunoimaging, and is especially suitable for high-sensitivity detection of low-expression protein markers.

The utility model provides a microwave receiving module, which relates to the technical field of signal processing, and comprises a VGC control temperature compensation circuit, the VGC control temperature compensation circuit comprises a voltage-controlled amplifier and a temperature sensor, and the input end and the output end of the voltage-controlled amplifier are respectively connected with an amplitude-limiting low-noise amplification filter circuit and a noise control circuit. The power supply end of the voltage-controlled amplifier is connected with the voltage conversion circuit, and the temperature sensor is connected with the control voltage input end of the voltage-controlled amplifier through the signal processing circuit. The problem of gain fluctuation caused by electrical characteristic change of active devices such as a transistor and an amplifier chip in the VGC amplification control circuit due to temperature change is effectively solved, the control voltage of the voltage-controlled amplifier is dynamically adjusted, and the voltage-controlled amplifier can stably work at different temperatures; therefore, the gain stability of the whole microwave receiving module is improved, and the signal amplification effect is improved.

The application provides a microfluidic chip for SDMA optical chemiluminescence detection and a use method, relates to the field of biomedical detection, and the microfluidic chip comprises a fan-shaped substrate, a sample introduction unit and a diluent unit are arranged on the fan-shaped substrate, a mixing groove is arranged on the side of the diluent unit away from the center of the fan-shaped substrate, and the mixing groove is connected with the sample introduction unit and the diluent unit; a plurality of detection units are arranged on the side of the mixing groove away from the center in a circumferential direction, each detection unit comprises a first reaction groove and a second reaction groove arranged in a radial direction of the fan-shaped substrate and connected with each other, and the first reaction groove is connected with the mixing groove; a first compound and a second compound are arranged in the first reaction groove, and a third compound is arranged in the second reaction groove; by arranging the two reaction grooves, the competition reaction is ensured not to be disturbed by a subsequent signal amplification step in space, so that hook effect is effectively avoided, liquid crosstalk to adjacent detection units is avoided, and the independence of each detection unit and the accuracy of results are ensured.

The invention relates to a kit and a method for detecting phosphorylated Tau protein. In particular to a p-Tau217 protein detection kit and a p-Tau217 protein detection method. The kit and the method disclosed by the invention are based on the p-Tau217 antibody and the total-Tau protein antibody, and are combined with a TSA technology, so that the specificity, the sensitivity and the accuracy of a detection result are improved, and background signals are effectively reduced.

This utility model relates to the field of precision instrument measurement technology, and in particular to a high-resolution grating subdivision module and an analog grating ruler. The high-resolution grating subdivision module, applied to a grating ruler, includes a signal input interface, a central control unit, a high-speed signal amplification unit, and a signal output interface. The central control unit incorporates a ZYNQ series processor. The signal input interface acquires analog signals from external devices and transmits them to the high-speed signal amplification unit, which uses a subdivision algorithm to process the signals to sub-nanometer resolution. Subsequently, the ZYNQ series processor within the central control unit utilizes an FPGA to perform high-speed parallel acquisition and adjustment processing on the subdivided high-speed analog signal, ultimately outputting a transmission signal with a communication rate of up to 1.25G through the signal output interface, thus achieving both high output rate and high-precision long-distance transmission.

The invention relates to a detection method, in particular to a method for rapidly detecting alpha and beta-lactoglobulin in auxiliary material lactose based on a microarray chip technology. On the basis of an indirect competitive enzyme-linked immunosorbent assay principle and a silver enhanced developing technology, a high-sensitivity microarray chip detection platform is successfully constructed by virtue of a biological chip scanner, and rapid quantitative analysis of alpha-lactalbumin and beta-lactoglobulin in the auxiliary material lactose for injection is realized. According to the method, an antigen-antibody specific recognition mechanism and a nano-silver signal amplification mechanism are integrated, and the detection efficiency and sensitivity are remarkably improved through the high-throughput layout of the microarray chip. In the aspect of methodology performance, the technology has the remarkable advantages of simple and convenient operation process, short detection time, high sample treatment flux, excellent specificity (CRlt; 1%) and the like.