15 results about "Biological target" patented technology

The present invention relates to the field of therapy and drug delivery. More specifically, therapeutic methods and pharmaceutical compositions for treating disorders of blood-tissue barrier-protected organs that harbor substantial populations of postmittal neurons, such as organs derived from the neural tube, including the central nervous system and the eye. The disclosed methods and compositions involve topical administration of an effector component that targets intracellular biological targets to such organs, in combination with a saponin component that enhances the effective uptake of the effector component into cells and / or enhances the effective delivery of the effector component within cells where the biological target is present. For example, the effector component may be an oligonucleotide therapeutic that targets gene products associated with CNS and / or ocular disorders. Due to the cellular uptake stimulating and / or endosomal escape enhancing effects of the saponin component, the neuropharmaceuticals and ophthalmic compositions presented herein for topical administration to the CNS and / or eye, respectively, can be formulated with lower concentrations of the effector component and / or lower volumes, which provides safety benefits to neurons and patient comfort.

The present application relates to image analysis technology, disclose a kind of water rare biological detection method and system based on underwater image analysis, comprising: monitoring target water area in preset time period in light signal data stream and spectrum data stream, identify the light signal data stream light emitting event, obtain light emitting event timestamp sequence, based on light emitting event timestamp sequence obtain light emitting image sequence in light signal data stream and spectrum data sequence in spectrum data stream, to light emitting image sequence and spectrum data sequence is carried out denoising correction and space-time synchronization processing, obtain after alignment image block sequence and after alignment spectrum curve, according to after alignment spectrum curve constructs spectrum attention weight, to after alignment image block sequence is carried out multi-scale morphological feature extraction, obtain multi-scale morphological feature map, to multi-scale morphological feature map is carried out rare biological target detection, obtain target detection result.The present application can improve the accuracy of water rare biological detection.

The application discloses a kind of asynchronous fusion method and system of deep-sea biological space-time trajectory analysis, the method is extracted from the single video data stream collected by underwater equipment biological target using visual detection module concurrently, and using video text recognition module extracts the space-time metadata superimposed in video picture;Then using asynchronous data fusion module based on a confidence-aware asynchronous extended Kalman filtering algorithm, the visual feature information and space-time metadata are fused, and the unified space-time trajectory point with geographic registration is generated.The application solves the technical problems that biological visual information and accurate space-time information are difficult to automatically integrate from single video source in the prior art, and through innovative asynchronous data fusion algorithm and diversified visualization output, the robustness, accuracy and application breadth of the automatic ecological monitoring and analysis system are significantly improved.

This invention relates to the field of bio-intelligent manufacturing technology, and particularly to an intelligent manufacturing equipment and method for a multi-biological targeted nutritional protection compound preparation. The equipment includes a manufacturing shell, the interior of which is equipped with an AI molecular design and virtual screening unit, an intelligent targeted delivery system preparation unit, a multi-scale online quality monitoring module, a four-biological effect simulation and verification unit, and a central control and digital twin server. In this invention, when vibrations generated during the repeated reciprocating motion of the microfluidic sampling needle and the use of other components cause a misalignment between the microfluidic sampling needle and the support frame, the pointer slides on the surface of the scale plate. The deviation distance is magnified and displayed using a magnifying glass. Then, a drive source is activated, causing the threaded rod to rotate, which in turn moves the pull plate left and right. This pushes the moving frame, the moving plate, and the magnetic block to slide on the electromagnet, thereby adjusting the position of the support frame and the microfluidic sampling needle, and returning the pointer to its initial position.

Disclosed are biosensor chip systems, kits, and methods usable for identifying, quantifying, and / or monitoring at least one target nucleic acid sequence of at least one microbial target in at least one sample. The systems employ nucleic acid-based affinity moieties coupled to electrodes to generate an electrical signal indicative of target presence and / or amount. In some embodiments, multiplex configurations enable simultaneous detection of multiple microbial targets in a single assay for rapid screening and longitudinal monitoring across clinical, environmental, and food-related matrices.

The present disclosure is directed to methods and / or uses of oligonucleotide conjugates for assays and flow cytometry detections and related systems and / or kits. Certain methods are directed to a method for detecting one or more biological targets of a sample in a detection assay, comprising: providing a molecular probe, comprising a binding moiety and an oligonucleotide sequence, to a sample comprising one or more biological targets; binding the one or more biological targets with the binding moiety; providing a detectable component to the sample, wherein the detectable component comprises a signal generating moiety conjugated to an oligonucleotide sequence complementary to the oligonucleotide sequence of the molecular probe; hydridizing the oligonucleotide sequence of the target-bound molecular probe to the detectable component; and detecting a signal generated from the hydridized detectable component. Various other embodiments, applications etc. are disclosed herein.

This invention discloses a solution for inhibiting keloids and its preparation method. Physiological saline is treated with low-temperature atmospheric pressure plasma (CAP) to become activated saline. The activated saline contains a large amount of reactive oxygen species and reactive nitrogen species. After injection into the body, its active ingredients induce disulfide death in fibroblasts derived from keloids, thereby inhibiting keloid formation. Compared with existing methods for inhibiting keloids, this application has several unique advantages. First, the effect and degree of CAP activation on fibroblast life and death are dose-dependent, allowing for precise control of the effect. Second, the full spectrum of active species generated by CAP can synergistically act on multiple biological targets, reducing the risk of recurrence. Third, CAP is administered through the process of generating an activated solution from a liquid medium, enabling non-invasive or minimally invasive drug delivery and greatly reducing the risk of systemic toxicity. Finally, CAP technology avoids complex biological agent preparation processes, making it simpler to operate and lower in cost. This invention fills the gap in research on low-temperature atmospheric pressure plasma inhibition of keloids.

The application discloses an electrochemical biosensor, which comprises at least one sensor unit; the sensor unit comprises a substrate, a channel layer arranged on one side of the substrate, a source electrode and a drain electrode electrically connected with the channel layer respectively, and a first gate electrode arranged on the side of the channel layer away from the substrate; at least part of the surface of the first gate electrode is provided with one or more connecting molecules, one end of the connecting molecule is connected with the first gate electrode through a metal-sulfur bond, and the other end of the connecting molecule is connected with a first recognition molecule through an amide bond; the first recognition molecule is used for binding a target object. The electrochemical biosensor contains the connecting molecules, when the target object is detected, the connecting molecules in the electrochemical biosensor can be connected with the target object through the recognition molecules, so that the electrical signal of the detection of the target object is amplified, and the electrochemical biosensor has the advantages of high sensitivity and high specificity.

The application discloses a brain tumor disease treatment effect evaluation method, and belongs to the technical field of radiotherapy plan evaluation, and specifically comprises the following steps: acquiring patient brain multi-modal image data and extracting three-dimensional tumor regions and corresponding perfusion parameters; constructing a biological target region hierarchical structure containing tumor overall regions and hypoxic subregions; obtaining a relative hypoxia degree parameter by calculating the cerebral blood flow ratio of the hypoxic subregion to other regions of the tumor; deconstructing historical treatment case data to establish a dose-therapeutic effect correlation parameter set; training a dose adjustment strategy model for the hypoxic subregion; and generating a final radiotherapy dose plan scheme according to the dose adjustment value output by the model combined with a clinical guideline basic dose. The application overcomes the limitations of traditional uniform dose irradiation through a data-driven dose decision mechanism, thereby providing an objective and reliable evaluation basis for clinical selection and optimization of personalized treatment schemes.

This invention discloses an automated cell manipulation method and system guided by artificial intelligence, comprising: identifying and locating the biological target object to be manipulated based on real-time images acquired by a microscopic imaging system to obtain target position information; calibrating and tracking the end-effector position of a micromanipulator based on the real-time images to obtain the real-time end-effector position; planning and dynamically correcting the motion trajectory of the micromanipulator based on the target position information and the real-time end-effector position until the end-effector reaches the preset target operation position, and controlling the micromanipulator to execute a preset interactive operation; verifying the operation result based on the real-time image after the operation to obtain the verification result, and controlling subsequent processes based on the verification result. This invention overcomes the inherent defects of traditional manual operation and semi-automated solutions in microscale cell manipulation through an end-to-end intelligent process, achieving high-precision, high-throughput, high-success-rate, and highly standardized cell manipulation.

The application discloses a deep-sea rare biological target detection method based on a stable diffusion model and belongs to the technical field of deep-sea biological target detection. Rare biological categories are determined from a deep-sea benthic biological real scene data set, and a foreground region image is extracted to construct a real target data set, a foreground image generation model is trained, and diversified synthetic foreground images are generated. A conditional control network is used to generate an external drawing of the foreground image according to a layout mask, the synthetic foreground image is fused with a deep-sea background, and a target position label is automatically generated according to the layout mask to obtain a synthetic scene image. After the synthetic quality is screened and the label frame is refined, the synthetic scene image is combined with the real scene data set to construct an enhanced training data set, and a target detection model is trained. The application solves the problem that the target detection performance is limited due to the long-tail distribution effect of the deep-sea exploration data set, and improves the detection capability of the target detection model for the rare biological categories.

This invention discloses a system and method for multi-channel vascular and multi-organ vascular-vascular fluid interfaces in in vitro models. The method includes: embedding at least one tissue in a 3D structure; forming a vascular interface for perfusing nutrients into the one or more tissues; modulating the one or more vascular interfaces, together or separately, by altering vascular properties to possess characteristics of a natural biological target tissue or target environment; and lining the fluid interface channels to provide growth fluid in a physiologically generalized manner. The 3D structure includes vascular regeneration elements for vascular regeneration or vascular formation.

A method for culturing a tissue using a microphysiological device comprising: embedding at least two tissues into a 3D structure, wherein the 3D structure includes a vasculogenic element; forming a vascular interface for nutrient perfusion into the at least two tissues; tuning the vascular interface to possess a property of a native biological target tissue or target environment by modifying a vessel attribute; and lining a fluidic interface channel, coupled to the 3D structure for providing a growth fluid.

Described herein are compositions and techniques related to generation and therapeutic application of engineered extracellular vesicles. Engineered extracellular vesicles are vesicles (i.e., exosomes) comprising at least one engineered fusion polypeptide further comprising a vesicle targeting domain, scaffold linker domain, and signaling domain (i.e., a multi-effector signaling domain) wherein the signaling domain targets and interacts with a biological target, such as cell surface receptors. Said engineered fusion proteins can be organized in genetic vector constructs, expressed in mammalian cells, wherein the vesicle targeting domains anchor to extracellular vesicles such as exosomes, thereby presenting their joined multi-effector signaling domain which can relay a signal to target cells. Engineered extracellular vesicles adopt the hallmark biophysical and biochemical features of extracellular vesicles, allowing for rapid deployment and scale-up. Importantly, this strategy can allow for kinetically favorable signal generation and signal propagation to a target cell.

The application discloses a kind of water product cultivation monitoring cross-media aircraft and method, the water product cultivation monitoring cross-media aircraft including shell and annular rotor being arranged in the four around shell, high-definition integrated camera is equipped on shell, underwater main propeller is equipped in the tail of shell, the bottom of shell is equipped with landing gear, environmental monitoring sensor module, feeding device;Underwater main propeller rear is further equipped with rudder, and rudder is connected with the tail of shell by fixed support.The application can be well adapted to the cross-media movement in air and underwater, can effectively complete the feed delivery in suitable position according to specific conditions, break through the limitation of single data source to breeding scene, form "air-water-biology" stereoscopic perception;Especially in underwater environment, significantly reduce the interference to fish group and other biological targets, improve the effectiveness and reliability of image acquisition and monitoring result.