Biosensor based on aptamer modified conducting polymer and its preparation method and uses

A technology of biosensors and conductive polymers, applied in biochemical equipment and methods, measurement/testing of microorganisms, instruments, etc., can solve problems such as complex technology, limited sensitivity, unfavorable sensing elements, etc., and achieve strong specificity and affinity , a wide range of applications, easy to synthesize the effect

Inactive Publication Date: 2008-02-20
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the above studies, the former detected the H generated during the biochemical reaction. 2 o 2 , not directly for the determination of small molecule glucose, the detection concentration range given by the experiment is 2-9 mmol / L, and the sensitivity is limited
However, the fabrication method and modification method of the sensor proposed by the Lieber research group require relatively complex technologies, which is not conducive to the simple and easy realization of in-situ controllable preparation of sensing elements and the simultaneous measurement of multiple components.

Method used

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  • Biosensor based on aptamer modified conducting polymer and its preparation method and uses
  • Biosensor based on aptamer modified conducting polymer and its preparation method and uses
  • Biosensor based on aptamer modified conducting polymer and its preparation method and uses

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Experimental program
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Embodiment 1

[0054] 1. Fabrication of Microelectrode Arrays

[0055] Thermal oxidation is performed on the surface of the Si wafer to obtain SiO 2 Oxide layer, the thickness is controlled at 500 nanometers. Vapor-deposit 5 nanometers thick Ti on the surface of the thermal oxide layer of the above-mentioned Si wafer, and then perform photoetching to obtain a microelectrode of the expected shape consisting of a working electrode and a counter electrode, wherein the working electrode and the counter electrode are respectively composed of 10 electrode wires , the width 1 of the electrode wires of the working electrode and the counter electrode is 10 microns, the spacing 2 between the electrode wires forming the working electrode or the counter electrode is 2 microns, and the row spacing 3 between each electrode wire is 100 microns; In the electron beam deposition system, Pt is deposited on the surface of Ti, and the thickness is controlled at 30 nanometers to obtain a microelectrode array tha...

Embodiment 2

[0066] (1) Two microelectrodes A and B are connected in parallel, first apply the method described in Example 1 between the A microelectrodes to prepare a biosensor with thrombin nucleic acid aptamer modification, the only difference between the experimental conditions and Example 1 is When preparing the electrolyte solution in the first step, add 25 μl of the pyrrole solution with a concentration of 5.0 mmol / L to which the thrombin nucleic acid aptamer is linked instead of 50 μl (the molar ratio of the conductive polymer monomer to the conductive polymer monomer linked to the nucleic acid aptamer is 800:1); then adopt the same three-step polymerization method between microelectrodes B, just replace the pyrrole monomer modified by the thrombin nucleic acid aptamer with the pyrrole monomer modified by the IgE (immunoglobulin E) nucleic acid aptamer body, biosensors with IgE nucleic acid aptamer modification can be obtained. A simple biosensor array capable of recognizing two bi...

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Abstract

The utility model relates to a biosensor, which utilizes the specific identification of the aptamer on target biomolecule and the changes in the electric conductance property of organic productive polymerization caused by the aptamer to do detection on the biological target quantitatively. The microelectrode of the biosensor comprises a working electrode and a counter electrode; wherein, the working electrode or the counter electrode respectively comprises a plurality of electrode wires, which are the same in number and in relative array, an in situ synthesized aptamer decorated organic conductive polymerization film or nano-wire is connected between the electrode wires of the working electrode and the electrode wires of the counter electrode, and the working electrode and the counter electrode are connected with a conductor respectively. By detecting changes in the electric conductance property of the organic productive polymerization caused by the specific combination of the target biomolecule and the aptamer on the organic conductive polymerization which is fixed between the microelectrode pairs, the utility model gets the quantitative response to the bio-macromolecules, such as the target protein, directly and fast, and avoids the complex and time consuming marking and incubation in the traditional method.

Description

technical field [0001] The invention belongs to the field of resistive biosensors, and relates to the specific recognition of target biomolecules by using nucleic acid aptamers (Aptamers, nucleic acid recognition bodies, aptamers), and the changes in the conductivity properties of organic conductive polymers caused by the nucleic acid aptamers to realize the detection of biological targets. Biosensors for quantitative detection, especially related to the recognition and concentration detection of biological macromolecular proteins, polypeptides, polysaccharides or cells, viruses, etc. Background technique [0002] Biosensors are expected to change the traditional biochemical analysis methods, and are widely used in clinical testing, biochemical analysis, biological research and quality control of biochemical products as a convenient and fast analytical means and equipment. In the current research, traditional biosensors are based on the specific recognition of biomolecules s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/403G01N27/327G01N27/27C12Q1/68
Inventor 方晓红侯双
Owner INST OF CHEM CHINESE ACAD OF SCI
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