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45results about How to "Does not affect the determination" patented technology

Biosensor based on aptamer modified conducting polymer and its preparation method and uses

The utility model relates to a biosensor, which utilizes the specific identification of the aptamer on target biomolecule and the changes in the electric conductance property of organic productive polymerization caused by the aptamer to do detection on the biological target quantitatively. The microelectrode of the biosensor comprises a working electrode and a counter electrode; wherein, the working electrode or the counter electrode respectively comprises a plurality of electrode wires, which are the same in number and in relative array, an in situ synthesized aptamer decorated organic conductive polymerization film or nano-wire is connected between the electrode wires of the working electrode and the electrode wires of the counter electrode, and the working electrode and the counter electrode are connected with a conductor respectively. By detecting changes in the electric conductance property of the organic productive polymerization caused by the specific combination of the target biomolecule and the aptamer on the organic conductive polymerization which is fixed between the microelectrode pairs, the utility model gets the quantitative response to the bio-macromolecules, such as the target protein, directly and fast, and avoids the complex and time consuming marking and incubation in the traditional method.
Owner:INST OF CHEM CHINESE ACAD OF SCI

Preparation method and application of BODIPY (boron-dipyrromethene) and Cys (cysteine) fluorescent probe

The invention discloses a preparation method and an application of a boron-dipyrromethene (BODIPY) based fluorescent probe for determining cysteine (Cys). The structural formula of the probe is shown in the specification. The preparation method of the BODIPY derivative based Cys fluorescent probe with a simple structure is provided, and the BODIPY derivative based Cys fluorescent probe is used for directly measuring Cys concentration. In the system, the fluorescent probe shows good selectivity and is not interfered with other biological thiols (Hcy and GSH) and other 19 amino acids. The probe shows quite high sensitivity to Cys, and the fluorescence intensity is enhanced by 23 times when 3 equivalents of Cys are added. When the pH is between 6.0 and 8.0, determination of Cys by the fluorescent probe is not affected by pH. The fluorescence probe and Cys act quickly, and the response time is within 6 minutes. In addition, the probe can also be used in cell imaging to detect intracellular Cys.
Owner:XIANGTAN UNIV

Method for determining content of ferric iron in lithium iron phosphate

The invention provides a method for determining the content of ferric iron in a cell anode material LiFePO4. The method utilizes 2-pyridylaldehyde-p-phenyldihydrazone as a fluorescence reagent to detect the content of ferric iron in the LiFePO4 material. The result of the determination of the content of the ferric iron in LiFePO4 in the prior art is easily influenced because of the existence of ferrous iron. The method provided by the invention adopts the combination of ferric iron with a Schiff base compound 2-pyridylaldehyde-p-phenyldihydrazone to quench the fluorescence of 2-pyridylaldehyde-p-phenyldihydrazone, the attenuation intensity of the fluorescence of 2-pyridylaldehyde-p-phenyldihydrazone is in direct proportion to the concentration of ferric iron ions, and other irons comprising ferrous ion and the like do not cause the fluorescence quenching of 2-pyridylaldehyde-p-phenyldihydrazone, so the method based on the fluorescence intensity attenuation of 2-pyridylaldehyde-p-phenyldihydrazone can quantitatively detect the content of ferric iron in LiFePO4.
Owner:CHANGZHOU UNIV +1

Device and method for analyzing trace impurities in gas

The invention discloses a device and a method for analyzing trace impurities in gas, and belongs to the field gas chromatography. The device comprises a switching valve I, a switching valve II, a switching valve III, a switching valve IV and four gas carrying channels carried with gas I, gas II, gas III and gas IV respectively, wherein two quantitative pipes, namely a quantitative pipe I and a quantitative pipe II, are arranged on the switching valve I; a first molecular sieve column is arranged between the switching valve I and the switching valve II; a second molecular sieve column is arranged between the switching valve III and the switching valve IV; a Porapak Q column is arranged between the switching valve I and the switching valve IV; a deoxygenation column and a three-way switch valve are arranged on the switching valve III. The invention further provides a method for analyzing the trace impurities in gas. According to the device and the method provided by the invention, the analysis of all of the trace impurities can be completed only by one-time sampling; trace impurities in various high-purity gas, ultra-pure gas and mixed gas, including oxygen, can be analyzed, so that the analysis cost is reduced, the sampling operation is simplified, and the analysis speed is increased.
Owner:PERIC SPECIAL GASES CO LTD

Method for analyzing composition of alcohol-containing gasoline and alcohol adsorption material used thereby

The multidimensional gas chromatographic process of analyzing the composition of alcohol containing gasoline includes injecting gasoline sample into multidimensional gas chromatograph to separate aliphatic hydrocarbon and arene from alcohol, leading the aliphatic hydrocarbon flowing out from the polar chromatographic column to olefin well for adsorbing olefin therein, detecting the saturated hydrocarbon from the olefin well, detaching olefin well from the carrier gas flow path, detecting benzene in alcohol well, reverse blowing the polar chromatographic column with carrier gas, adsorbing alcohol in the alcohol well, detecting arene, desorbing and detecting olefin and desorbing and detecting alcohol. The alcohol adsorbing material includes CaCl2 20-50 wt% and diatomite as carrier 50-80 wt%. The said process can detect hydrocarbon, benzene, arene, olefin and alcohol content in gasoline effectively.
Owner:CHINA PETROLEUM & CHEM CORP +1

Preparation method of hydrogel cataplasm and prepared cataplasm with effect of relieving swelling and pains

The invention relates to a preparation method of hydrogel cataplasm and prepared cataplasm with the effect of relieving swelling and pains. The prepared cataplasm comprises glycerol, polyvinylpyrrolidone, crosslinked povidone, sodium polyacrylate, aluminum glycinate, sodium carboxymethylcellulose, ethanol, borneol, citric acid, polyvinyl alcohol, sodium benzoate and purified water. The preparation method comprises the following steps: (1) dissolving borneol in ethanol for later use; (2) dissolving citric acid in 14.5-35 parts of water for later use; (3) dissolving sodium benzoate in water, adding polyvinyl alcohol, and heating to dissolve; and adding the rest purified water; and (4) pouring glycerol into a container, sequentially adding polyvinylpyrrolidone, crosslinked povidone, sodium polyacrylate, aluminum glycinate and sodium carboxymethylcellulose, stirring uniformly, adding the borneol ethanol solution, and stirring uniformly; and obtaining a gel. The preparation method provided by the invention is convenient, and has the advantages of simple process, operation at room temperature, environmental friendliness, high safety, no environmental pollution, and no waste emission during the production process. The entire process is harmless to operators.
Owner:TIANJIN RUIDE PHARMA

Method for detecting content of heavy metals

The invention provides a method for detecting a content of heavy metals, which belongs to the technical field of the detection of metal ions. The method comprises the following steps: (1) preparing asolution to be detected: preparing a heavy metal sample to be detected into an aqueous solution, adding an acidic solution and a potassium iodide solution, and obtaining the solution to be detected; (2) detecting the solution to be detected: moving the solution to be detected, dropwise adding the solution to be detected onto test paper, developing the color for 50s to 100s, wherein the test papercontains dye; and (3) judging a detection result: visually comparing the color developing test paper and a colourimetric card, and determining a concentration of the heavy metal. By adopting the method for detecting the heavy metals, a rapid detection effect can be achieved, the detection efficiency is increased, and the field operation is also facilitated.
Owner:HEZHOU UNIV

Lyophilized heparanase preparation, heparanase cup as well as preparation method and application of lyophilized heparanase preparation and heparanase cup

The invention relates to the technical field of in vitro diagnostic reagents, and particularly relates to a lyophilized heparanase preparation, a heparanase cup as well as a preparation method and application of the lyophilized heparanase preparation and the heparanase cup. A redissolved enzyme activity yield of the lyophilized heparanase preparation provided by the invention is at least 75 percent. The heparanase cup provided by the invention comprises a cup body and the lyophilized heparanase preparation, wherein an accommodating space is defined in the cup body; the lyophilized heparanase preparation is arranged in the accommodating space. The invention also provides a method for preparing the lyophilized heparanase preparation. The method comprises the following steps: sequentially performing pre-freezing treatment, sublimation treatment and drying treatment on aqueous solutions prepared from heparinase, a protective agent, buffer salt and a preservative in a predetermined proportion so as to obtain the lyophilized heparanase preparation. The lyophilized heparanase preparation and the heparanase cup are used in a detection process of blood samples, and the detection sensitivityis high; moreover, the activity and the stability of the heparinase in a lyophilization process can be obviously improved.
Owner:深圳优迪生物技术有限公司

Simple and efficient method for detecting concentration of venlafaxine and active metabolite O-desmethylvenlafaxine in human blood serum

InactiveCN108982714AStrong specificityStrong specificity and high sensitivityComponent separationFluorescenceMedicine
The invention provides a simple and efficient method for detecting the concentration of venlafaxine and an active metabolite O-desmethylvenlafaxine in human blood serum and relates to the technical field of medicines. The simple and efficient method is finished through the following steps: (1) treating a blood serum sample; (2) preparing a solution; and (3) selecting chromatographic conditions. According to the simple and efficient method provided by the invention, a blood sample treatment process is optimized and the simple and efficient method is simple to operate and low in cost; a fluorescence method has strong specificity and high sensitivity; and a capability of accurately determining the venlafaxine and the active metabolite O-desmethylvenlafaxine is greatly improved and the reliability of the determination method is improved.
Owner:SHANGHAI MENTAL HEALTH CENT (SHANGHAI PSYCHOLOGICAL COUNSELLING TRAINING CENT)

Combustion detection system and combustion detection method thereof

The invention discloses a combustion detection system and a combustion detection method thereof. The combustion detection system disclosed by the invention comprises a transmitting terminal fixing device, a rotating table I, a transmitting device, a laser switching device, an optical path calibration device, a receiving terminal fixing device, a rotating table II, an optical filter, a receiving device and a photoelectric detector; the rotating table I and the rotating table II are respectively fixed on the transmitting terminal fixing device and the receiving terminal fixing device; the transmitting device is fixedly arranged on the rotating table I; the optical path calibration device is detachably arranged on the transmitting terminal fixing device; the laser switching device is connected with the transmitting device by optical fibers; the optical filter and the receiving device are coaxially fixed on the rotating table II; the receiving device is connected with the photoelectric detector by optical fibers; when an optical path is calibrated, the transmitting device transmits indicating light, the indicating light passes through the optical path calibration device and a furnace sequentially, then is reflected by the optical filter and enters into the optical path calibration device for calibration of the optical path. The combustion detection system disclosed by the invention can carry out rapid alignment when a boiler is in a cold state.
Owner:NANJING SCIYON AUTOMATION GRP +1

Method for evaluation and quality control of vesicle separating efficiency based on liposome

The invention provides a method for evaluation and quality control of the vesicle separating efficiency based on liposome. The method comprises the following steps of: firstly utilizing lipids such asphosphatidylcholine, phosphatidyl ethanolamine and cholesteryl ester and the like and fluorescent dyes to prepare fluorescent liposome; after respectively mixing the fluorescent liposome with serum,plasma and urine samples, using different separation methods including ultracentrifugation, a membrane affinity method, polymer precipitation and chromatography and the like to separate vesicles; carrying out fluorimetric determination on the separated vesicles. The method provided by the invention has the beneficial effects that the extraction efficiency of the vesicles in different methods and the extraction efficiency in the treatment process of different clinical samples can be calculated by the fluorescence intensity of the original liposome and the fluorescence intensity of the separatedvesicles. The method utilizes the fluorescent liposome to evaluate the extraction efficiency of the vesicles, provides powerful technical support for applying the vesicles in a stable quality-controlsystem with clinical needs, and simultaneously provides an effective method for observing and tracking the separation process of the vesicles by naked eyes.
Owner:北京恩泽康泰生物科技有限公司

Method for simultaneously detecting main component contents of quinapril hydrochloride and hydrochlorothiazide composition

The invention provides a method for simultaneously detecting the main component contents of a quinapril hydrochloride and hydrochlorothiazide composition. The method adopts the high-efficiency liquid chromatography to simultaneously measure the contents of quinapril hydrochloride and hydrochlorothiazide at one time. The method comprises the following steps: adopting the high-efficiency liquid chromatography, using a cyano chromatographic column or a C8 chromatographic column, adopting a mobile phase composed of an organic phase and a water phase for isocratic elution, and introducing a sample at one time. Therefore, the detection effects of high precision and repeatability can be achieved. The method is simple, is easy and quick to operate, is stable and reliable, and has higher application value.
Owner:YANGZIJIANG PHARMA GROUP SHANGHAI HAINI PHARMA +1

Method for measuring content of tungsten and molybdenum in ore

The invention discloses a method for measuring the content of tungsten and molybdenum in ore. After being weighed, a sample is soaked in mixed acid of hydrochloric acid, nitric acid, hydrofluoric acid and perchloric acid, heated and dissolved at 200-230 DEG C, then disgested, and finally dried by distillation into a wet salt shape; finally, a complex is generated from tungsten through an oxalic acid and hydrochloric acid mixed solution. After capacity is fixed, an inductively coupled plasma-atomatic emission spectrometry (ICP-AES) is used for measuring the content of tungsten and molybdenum. By means of the method, the content of the two elements can be measured at the same time, and the problem that at present, the two elements can not be accurately measured at the same time is solved. In addition, by means of the method, high-temperature and strong-alkali operation is avoided, experiment procedures are simplified, experiment cost is reduced, test speed of the sample is increased, and work efficiency is effectively improved.
Owner:CHANGCHUN GOLD RES INST

New method for quickly determining fruit point density

The invention discloses a new method for quickly determining the fruit point density. The new method comprises the following steps: taking a plastic transparent board or a cardboard of 29 cm*21 cm, reserving an edge of 3-5 cm on each of the upper end, the lower end, the left end and the right end and forming a frame; then drawing a plurality of square small grids of 1 cm*1 cm in the frame; after the completion of drawing, hollowing out the one small grid every other small grid, so that a plurality of empty grooves of 1 cm<2> are formed, and a test template is obtained; during measurement, directly buckling the obtained test template on a fruit, wrapping the whole fruit in the template, respectively counting the number of fruit points on the corresponding empty slot per square centimeter to obtain the number of the fruit points per square centimeter. The new method disclosed by the invention has the benefits that the manipulative ability and the innovation ability of a student are enhanced, and more time is saved; the fruit appearance and the determination of other data are not affected; the determination method is simple and feasible, the template can be repeatedly used for multiple times, the determined data is accurate, and other fruits and vegetables, like applies, peaches and the like can also be determined.
Owner:TARIM UNIV

Method for determining antimony in lead bullion by precipitation separation-carbon reduction cerium sulfate volumetric method

The invention discloses a method for determining antimony in crude lead by a precipitation separation-carbon reduction cerium sulfate volumetric method, which comprises the following steps: dissolvinga sample with dilute nitric acid, selecting ammonia water or sodium carbonate as a precipitant, slowly dropwise adding the precipitant, regulating the pH value of the solution to 3-5, boiling for 5 minutes, and filtering after complete precipitation; dissolving the filter residues and filter paper with sulfuric acid, carbonizing the filter paper, directly reducing Sb < 5 + > into Sb < 3 + >, andtitrating antimony with a cerium sulfate standard solution in a sulfuric acid-hydrochloric acid medium. Ammonia water with the volume percentage concentration of 50% or a sodium carbonate solution with the mass-volume ratio of 200 g / L is adopted as a precipitator; antimony precipitates in the solution are completely separated from other interference elements; excessive precipitant reacts with sulfuric acid or hydrochloric acid to generate water or carbon dioxide, antimony precipitate and filter paper are dissolved in concentrated sulfuric acid together after filtration and can be directly usedfor reducing antimony after being carbonized by the concentrated sulfuric acid, loss caused in the ashing or precipitate redissolving process is avoided, and the detection accuracy and precision aregreatly improved.
Owner:NORTHWEST RES INST OF MINING & METALLURGY INST

Composition for preventing and controlling white leaf spot of strawberry

The invention provides a composition for preventing and controlling white leaf spot of strawberry. The composition is prepared from an active ingredient group A and an active ingredient group B. The invention also discloses a bactericide for preventing and controlling white leaf spot of strawberry, which contains the composition. According to the composition provided by the invention, components in the active ingredient group A are plant-sourced and thus being safe and environment-friendly to avoid environmental pollution and adverse impact on strawberries. When the white leaf spot occurs, through cooperation of the active ingredient group B, the antibacterial effect is more thorough.
Owner:淮南市永光草莓专业合作社

Application of polygold/acetylene black composite film modified glassy carbon electrode in detecting hydroquinone

The invention discloses a preparation method of a polygold / acetylene black composite film modified glassy carbon electrode, and discloses an application of a polygold / acetylene black composite film modified glassy carbon electrode in detecting hydroquinone at the same time. In the application of the polygold / acetylene black composite film modified glassy carbon electrode in detecting hydroquinone,the glassy carbon electrode modified by the acetylene black / nanogold composite material is a working electrode, which significantly improves the sensitivity and linear range of the analysis method, and has advantages of good stability, easy preparation, low cost, good reproducibility and the like.
Owner:XINYANG NORMAL UNIVERSITY

Non-water leucorrhea specimen storing liquid and preparation method and pH value test collection tube

The invention discloses a non-water leucorrhea specimen storing liquid and a preparation method and pH value test collection tube of the liquid. The non-water leucorrhea specimen storing liquid comprises the following substances by weight percent: 96.5wt% of anhydrous glycerin, 0.2wt% of polyethyleneglycol 8000 as a surface active agent, 0.25wt% of bromocresol green, 0.05wt% of methyl red, 2.0wt% of absolute ethyl alcohol and 1.0wt% of mercapto sodium acetate. The non-water leucorrhea specimen storing liquid has the advantages that visible components such as bacteriums, cells, trichomonads and sick bodies of the leucorrhea can be protected by anhydrous glycerin (glycerol) when being frozen at a temperature less than -20 DEG C; the cell completeness of the visible components such as cells, bacteriums, trichomonads and the like can still be maintained after being redissolved without changing the morphology under a microscope; the leucorrhea can be solved in water in any concentration when needing to be diluted; mercapto sodium acetate is used as a reducing agent to prevent the specimen from inactivation caused by dehydration, oxidation and autolysis during the storage or transportation; and the non-water leucorrhea specimen storing liquid does not affect the cell lysis and determination of Chlamydia trachomatis or various causative agents of the leucorrhea.
Owner:安徽信灵检验医学科技股份有限公司

Method for analyzing small-size conductive and non-conductive materials by applying glow discharge mass spectrometry

The invention relates to a method for analyzing small-size conductive and non-conductive materials by applying glow discharge mass spectrometry, and belongs to the field of material analysis. The method comprises the following steps: placing graphite powder in an aluminum cup, and slightly vibrating the aluminum cup to flatten the surface of the graphite powder; placing a small-size conductive material or a non-conductive material on the graphite powder; covering with a plurality of layers of parchment paper; pressing by using a tablet press to prepare a test sample; and carrying out direct current glow discharge mass spectrometry. According to the analysis method, grinding, sintering and heating are not needed, the pretreatment is simple, and the pollution is avoided; the problem that the metal element cannot be measured when the metal is used as a conducting medium and the mass spectrum interference formed by the metal element are avoided; the analysis time is shortened, and the analysis efficiency is improved; the method can be suitable for analysis of small-size conductive materials such as crumb-shaped, granular and powdery conductive materials and fine-granular and powdery non-conductive materials, and is high in universality and wide in application range; the method has good discharge stability, analysis repeatability and analysis accuracy.
Owner:国合通用测试评价认证股份公司 +1

Method for determining gallium content in antibacterial stainless steel

The invention belongs to the technical field of alloy macroelement analysis, and particularly provides a method for determining the gallium content in antibacterial stainless steel, so as to fill the blank of the current method for detecting the gallium content in the antibacterial stainless steel. The method comprises the following steps: dissolving gallium-containing antibacterial stainless steel by using aqua regia, eliminating iron matrix interference by using a matrix matching method, and eliminating ferrospectral line interference by using a matrix blank as a standard working curve blank, thereby establishing an analysis and detection method for determining the gallium content in the stainless steel by using the inductively coupled plasma atomic emission spectrometry. The method is easy and convenient to operate, the test result is good in precision and high in accuracy, and the detection requirement of gallium in novel antibacterial stainless steel can be met.
Owner:INST OF METAL RESEARCH - CHINESE ACAD OF SCI

Method for detecting nickel content in acid etching waste liquid

The invention relates to the field of acid etching waste liquid treatment, and discloses a method for detecting nickel content in acid etching waste liquid, which comprises the following steps: (1) adding iodide into the acid etching waste liquid, so that iodide ions can reduce part of copper ions to generate cuprous iodide precipitate; (2) diluting to a constant volume, and removing the cuprous iodide precipitate to obtain a clear liquid; and (3) detecting the nickel content in the clear liquid by using an atomic absorption spectrophotometer. When the method is used for detecting the contentof nickel in the acidic etching waste liquid, iodide ions in iodide react with copper ions to generate cuprous iodide precipitates, and meanwhile, nickel ions in the waste liquid do not react with iodide ions, so that interference of copper ions in the acidic etching waste liquid on nickel content detection is removed, and measurement of the content of nickel in the waste liquid is not influenced.
Owner:遂宁市瑞思科环保科技有限公司

Artificial liposome containing miRNA mimic and preparation method and application thereof

The invention provides an artificial liposome containing miRNA mimic and a preparation method and application thereof. The artificial liposome is prepared by mixing liposome with a lipid bilayer structure and miRNA mimic, wherein the miRNA mimic is a synthetic non-human miRNA and its derivatives. A synthetic liposome wrapped with the exogenous miRNA mimic is used for characterization of miRNA extraction efficiency of extracellular vesicle samples and quantitative analysis of extracellular vesicle miRNA in body fluid samples, thus providing strong technical support for stable quantitative detection of vesicle miRNA in clinical needs.
Owner:北京恩泽康泰生物科技有限公司

Method for determining bacterial amount in sodium alginate microcapsule

The invention provides a method for determining the bacterial amount in a sodium alginate microcapsule. For the sodium alginate microcapsule after immobilized fermentation, an anaerobic sodium citratesolution is used to dissolve the microcapsule under a heating condition. The dissolved solution is taken to determine the content of organic phosphorus. The amount of organic phosphorus in the capsule is used to characterize the bacterial amount in the capsule. Since the phosphorus content of bacteria is relatively stable, the amount of bacteria can be characterized by the amount of organic phosphorus. In addition, the sodium alginate envelope dissolution method provided by the invention can avoid the conversion of organic phosphorus to inorganic phosphorus when an envelope is dissolved, so that the determination of the content of total phosphorus and inorganic phosphorus is not affected. The entire determining process has the advantages of convenient operation, being fast and accurate and easy implementing.
Owner:FUZHOU UNIVERSITY

A device and method for analyzing trace impurities in gas

The invention discloses a device and method for analyzing trace impurities in gas, belonging to the field of gas chromatography analysis. The device includes: switching valve I, switching valve II, switching valve III, switching valve IV, and four carrier gas circuits of carrier gas I, carrier gas II, carrier gas III and carrier gas IV; There are two quantitative tubes, namely quantitative tube I and quantitative tube II; a first molecular sieve column is set between switching valve I and switching valve II, and a second molecular sieve column is set between switching valve III and switching valve IV. A Porapak Q column is arranged between switching valve I and switching valve IV, and a deoxygenation column and a three-way switching valve are arranged on switching valve III. The invention also provides a method for analyzing trace impurities in gas, which can complete the analysis of all trace impurities with only one sample injection, and can analyze various high-purity gases, ultra-pure gases and their mixed gases including oxygen. trace impurities, saving analysis costs, simplifying sample injection operations, and speeding up analysis.
Owner:PERIC SPECIAL GASES CO LTD
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