151 results about "BODIPY" patented technology

The invention belongs to the technical field of organic synthesis, and particularly discloses an ammonia aromatic alkene modified near-infrared BODIPY fluorescent probe as well as a preparation method and application thereof.The near-infrared BODIPY fluorescent probe is obtained by adopting 6-(dimethylamino) nicotinic aldehyde to directionally modify the alpha position of BODIPY and constructing an expanded conjugated system through Knoevenagel condensation. Wherein dimethylamino provides a strong electron donating effect, a molecular structure is twisted into a non-planar configuration due to steric hindrance of a pyridine ring, pi-pi accumulation is reduced, and an ACQ effect is inhibited. According to the structural modification strategy, significant red shift of absorption / emission wavelength is realized by expanding a molecular pi-conjugated skeleton, and meanwhile, the stability of the BODIPY core and the quantum yield are effectively enhanced. The probe molecule can specifically recognize the diphenylalanine dipeptide structural unit by utilizing the synergistic coordination effect of the 2-aminopyridine derivative and the BODIPY mother nucleus, so that the selective detection of the Alzheimer's disease biomarker Abeta42 is realized, and a novel optical detection tool is provided for the early molecular diagnosis and targeted therapy of AD (Alzheimer's disease).

The present invention provides a lipoprotein subtype detection kit, a preparation method and an application thereof, belonging to the technical field of biological detection. In the separating gel of the kit, BODIPY 505 / 515 is mixed, so that staining is not required during detection, and direct sample loading and electrophoresis can be carried out, reducing the operation steps and shortening the operation time, and improving the detection sensitivity. In addition, N,N'-bisacryloylpiperazine is mixed in both the separating gel and the stacking gel. The rigidity of the N,N'-bisacryloylpiperazine gel is used to reduce gel deformation and trailing phenomena during migration, significantly improving the resolution, and enhancing the stability and repeatability of detection. The detection kit provided by the present invention has the advantages of short detection operation time, high detection sensitivity, high resolution, good stability and repeatability.

The invention provides an aza-BODIPY molecule with NIR-II fluorescence emission and I-type photodynamic activity and a preparation method thereof. The aza-BODIPY molecule has good light absorption in a near-infrared (NIR) region, the emission peak of the aza-BODIPY molecule is prolonged to a near-infrared second region (NIR-II), and the aza-BODIPY molecule shows an ultrahigh NIR-II fluorescence quantum yield of 13%. Meanwhile, the photosensitizer prepared by adopting aza-BODIPY molecules can effectively generate I-type active oxygen species, namely superoxide anion free radicals, under the excitation of 808nm laser. On the basis, high-resolution tumor fluorescence imaging can be realized by utilizing NIR-II fluorescence of the aza-fluorine boron fluorescent sensitizer, and the aza-fluorine boron fluorescent sensitizer is used for accurate and efficient NIR-II fluorescence imaging mediated I-type photodynamic therapy of deep tumor tissues.

The invention relates to the technical field of biological medicines, and particularly discloses a BODIPY (boron dipyrromethene)-based compound and an application of the BODIPY-based compound in photodynamic oxidation of beta-amyloid protein (Abeta). The compound takes iodo-BODIPY as a photosensitive core and is connected with 4-t-butyloxycarboryl benzene as an A beta recognition group. The introduction of iodine atoms significantly enhances the intersystem crossing ability of molecules, so that the molecules have excellent reactive oxygen species (ROS) generation ability. The compound has high ROS yield, good optical stability and biocompatibility, can specifically target A beta aggregate, realizes photodynamic oxidative degradation, and provides a novel potential tool for treatment of Alzheimer's disease.

The invention belongs to the technical field of fluorescent probes, and discloses a fluorescent probe based on BODIPY targeted lipid droplets and application of the fluorescent probe. The structural formula of the fluorescent probe for targeting the lipid droplets is as follows: # imgabs0 # is as follows: due to strong electron withdrawing capability and relatively large steric hindrance of ester groups, the ester groups of the probe can rotate in an excited state, so that energy is lost through non-radiative transition, and fluorescence quenching is caused. The ester group of the probe can rotate in an excited state due to the strong electron withdrawing capability and large steric hindrance of the ester group, so that energy is lost through non-radiative transition, and fluorescence quenching is caused. And due to the introduction of the ester group, the fat solubility of the BODIPY dye is further enhanced, so that the lipid droplet targeting property of the probe is effectively improved, and no-wash imaging of lipid droplets is realized.

The invention discloses an aza-BODIPY biomedical phototherapy agent taking oxajulolidine as a donor and a preparation method thereof, and relates to a medical phototherapy agent for treating brain glioma and a preparation method thereof, and the prepared compound is prepared by introducing an oxajulolidine fragment with electron donating property into a key position of an aza-BODIPY skeleton, so that the aza-BODIPY biomedical phototherapy agent is obtained. The donor-acceptor type molecular near-infrared phototherapy agent with a new structure is constructed, has good near-infrared absorption and emission performance and excellent stability, and shows a remarkable effect in the field of cancer photodynamic (PDT)-photothermal (PTT) synergistic treatment. The structural categories of the near-infrared aza-BODIPY biomolecules are enriched, and a new molecular platform is provided for expansion and application of the near-infrared aza-BODIPY biomolecules in the fields of biological imaging, tumor diagnosis and treatment, functional materials and the like.

The present invention discloses a BODIPY-based ratiometric fluorescent probe for detecting sulfite, its preparation method, and application. The fluorescent probe is 2,8-bis(2-(benzothiazol-2-yl)-2-cyanovinyl)-10-(furan-2-yl)-1,3,7,9-tetramethyl-5H-dipyrroledifluoroborane complex. The present invention utilizes 2,8-diformyl-10-(furan-2-yl)-1,3,7,9-tetramethyl-dipyrroledifluoroborane as a raw material, and carries out a condensation reaction with 2-(benzothiazol-2-yl)acetonitrile to obtain the compound 2,8-bis(2-(benzothiazol-2-yl)-2-cyanovinyl)-10-(furan-2-yl)-1,3,7,9-tetramethyl-5H-dipyrroledifluoroborane complex (abbreviated as BODIPY-DBC). When a solution of the compound BODIPY‑DBC reacts with sulfite, the solution changes color from purple to yellow under sunlight; under ultraviolet light at a wavelength of 365 nm, the solution's fluorescence changes from orange to green. BODIPY‑DBC can sensitively detect sulfite content in solutions, with high sensitivity (detection limit of 33.12 nM), fast response, good selectivity, and a wide detection range. It holds great promise as a fluorescent probe for sulfite detection.

The invention discloses a high-activity BODIPY type photosensitizer targeting double organelles as well as a preparation method and application of the high-activity BODIPY type photosensitizer, and relates to the technical field of medical photosensitizers. The high-activity BODIPY type photosensitizer is prepared by the following steps: reacting 2, 4-dimethyl pyrrole with 4-bromobutyryl chloride to obtain a compound Br-BY containing carboxyl, then reacting with morpholine to obtain a morpholine substituted compound Mor-BY, and finally reacting with N-iodosuccinimide (NIS) to obtain a single atom I substituted BODIPY compound Mor-IBY, namely the target photosensitizer. The photosensitizer prepared by the invention can target mitochondria and lysosome in cancer cells at the same time, and can be excited by visible light; the compound has good water solubility, can generate a large amount of reactive oxygen species (ROS) under irradiation of green light (520 nm), shows excellent photodynamic activity to tumor cells, and has low toxicity to normal cells.

The invention discloses a BODIPY derivative, a nano preparation, a corresponding preparation method and application, and the BODIPY derivative is prepared by taking BODIPY as a mother nucleus and adjusting substituent groups and expanding a conjugated system. According to the BODIPY derivative and the nano preparation, the emission wavelength can extend to an NIR-II region, and the BODIPY derivative and the nano preparation can be used for preparing a medicine for inhibiting tumor cell growth or a near-infrared two-region fluorescence imaging agent.

The invention discloses a novel BODIPY-based near-infrared open-type fluorescent probe for detecting hypochlorous acid as well as a preparation method and application of the novel BODIPY-based near-infrared open-type fluorescent probe. The fluorescent probe is (5, 5-difluoro-1, 3, 9-trimethyl-7-(4-sulfur morpholinyl styryl)-5H-dipyrrolo-diazaborolo-penta [10] alkene-10-yl)-4-phenol, which is abbreviated as BOD-Y. The invention also discloses a preparation method of the fluorescent probe. The probe BOD-Y can specifically recognize hypochlorous acid, the color of a probe BOD-Y solution added with hypochlorous acid is rapidly changed from green to blue under sunlight, and the fluorescence is also changed from colorless to strong red fluorescence. The detection limit of the probe on hypochlorous acid is as low as 21nM, the probe shows a good linear relation within 0-35 [mu] M, and the response time is within 13 seconds. The probe shows good selectivity on hypochlorous acid, and is not influenced and interfered by other anions, cations, biological mercaptan, active oxygen and the like.

The present invention belongs to the field of photodynamic therapy technology, and more particularly to a BODIPY compound, a preparation method and its use as a photosensitizer. The present invention provides a series of novel small molecule compound photosensitizers with BODIPY dimer as basic skeleton and pure type I photodynamic. By the combined action of heavy atoms, 2,4,6-trimethylphenyl groups and limited structure R groups introduced at specific positions, the reactive oxygen species yield is increased, and the regulation of pure type I photosensitizer is carried out by regulating energy levels. Compared with common photosensitizer molecule indocyanine green (ICG), the reactive oxygen species yield is about 50 times that of ICG, has higher photothermal stability, can significantly increase the applicability of photodynamic agents, and the preparation method is simple, the reaction efficiency is high, the yield is high, and there is good industrial application prospect.

This invention discloses a novel iron-based supramolecular catalyst and its preparation method. A fluoroboron pyrrole (Bodipy) organic photosensitizer with strong visible light absorption and a long excited-state lifetime is covalently coupled to an inexpensive iron-based tetrapyridine catalyst via a click reaction, shortening the distance between the photosensitizer and the catalyst to construct a non-precious metal supramolecular photocatalytic system with strong visible light absorption. Using 1,3-dimethylbenzimidazole as a sacrificial agent, the catalyst exhibits high catalytic activity and selectivity in photocatalytic carbon dioxide reduction. The reaction system is low-cost and has promising application prospects.

The invention discloses an imidazolyl BODIPY derivative viscosity fluorescent probe with an aggregation-induced fluorescence enhancement (AIEE) effect and application thereof, the imidazolyl BODIPY derivative viscosity fluorescent probe is realized by the following method: taking a 2-formaldehyde BODIPY derivative (I), diphenyl ethanedione and aniline as raw materials, and carrying out Debus-Radziszewski imidazole synthesis reaction under the catalytic action of ammonium acetate, and the chemical structural formula of the imidazolyl BODIPY derivative viscosity fluorescent probe is as shown in formula (II). The preparation method is simple in reaction step, mild in reaction condition, simple and convenient to separate and purify and high in yield; the fluorescent probe has an aggregation-induced fluorescence enhancement effect, has high responsiveness to viscosity, can be used for detecting viscosity change in a solution and cells, and can provide an effective detection method for researching physiological action of viscosity in cells.

The invention discloses a near-infrared ratio type fluorescent probe for rapidly and reversibly detecting histamine (HA), a preparation method of the fluorescent probe and application of the fluorescent probe in dynamic cell imaging, and belongs to the technical field of fluorescence imaging and biosensing. The preparation method comprises the following specific steps: synthesizing aza-BODIPY molecules (1a-1d, 2a-2d) with different halogenations, and meanwhile, connecting the aza-BODIPY molecules with hydroxyl groups at 3 and 5 sites with a hydrophilic PEG (Polyethylene Glycol) fragment and halogen to obtain an HA probe HAPCyto with good water solubility; in addition, the hydroxyl aza-BODIPY molecules are continuously subjected to substitution, halogenation and hydrolysis reaction to obtain carboxyl-containing aza-BODIPY molecules, then the aza-BODIPY molecules are subjected to amidation reaction by utilizing the site, and different organic fragments are respectively connected to obtain a cell membrane targeted HA probe HAPPM, a golgi apparatus targeted HA probe HAPGA and a HaloTag HA fluorescent probe HAPHaloTag. When HA exists in a solution, the developed probe can realize rapid reversible detection of the near-infrared ratio of histamine, also shows good reversible property in mast cells, and can realize dynamic monitoring of histamine in cells.

The invention relates to the technical field of chemiluminescence probes, in particular to a synthesis method and application of a boron dipyrromethene framework near-infrared two-region chemiluminescence probe based on X-ray activation, and the chemical structural formula of the chemiluminescence probe is shown in the specification. A novel BODIPY compound which is high in quantum yield and has a strong or narrow emission band is used as a chemiluminescence substrate for a core framework, a core framework of an electron withdrawing group with longer emission wavelength, an electron donating group and two molecules with larger wavelength overlap ratio are subjected to one-time CERT effect to be efficiently combined into a single molecule, and 1,050 nm chemiluminescence which is higher than 970 nm is emitted.

The invention relates to the technical field of biomedical detection and functional organic fluorescent materials, in particular to synthesis and application of a single-molecule probe combining BODIPY (boron dipyrromethene) and a semi-cyano dye. Comprising the following steps: preparing an intermediate 1: reacting 4-hydroxybenzaldehyde with 2, 4-dimethyl pyrrole to generate BDP-OMe, and then reacting with pyridinium chlorochromate to obtain the intermediate 1; preparation of an intermediate 2: carrying out reflux reaction on 2, 3, 3-trimethyl-3H-indole and 2-bromoethanol in ethanol to obtain the intermediate 2; and synthesizing a final product: heating the intermediate 1 and the intermediate 2 in toluene to react, and performing post-treatment and column chromatography purification to obtain the probe CyB-C. The bimodal detection capability enables the probe to simultaneously distinguish the microenvironment difference between normal cells and cancer cells and monitor diseases related to viscosity abnormity.

The invention belongs to the technical field of fluorescent dyes, and particularly relates to a phenanthroline-based double-BODIPY fluorescent dye with an asymmetric structure as well as a preparation method and application of the phenanthroline-based double-BODIPY fluorescent dye. The preparation method comprises the following steps: mixing a chlorinated BODIPY derivative with 1, 10-phenanthroline-5-amino, adding palladium acetate, tri-tert-butylphosphine, potassium tert-butoxide and toluene, and carrying out a heating reflux reaction; and after the reaction is finished, cooling, reducing pressure to remove toluene, dissolving the obtained product with dichloromethane, washing, drying, concentrating, and separating and purifying through silica gel column chromatography to obtain the target dye. The unique asymmetric structure in the prepared target dye destroys the planarity and symmetry of molecules, effectively weakens the fluorescence quenching effect caused by molecular self-absorption and pi... pi accumulation, and further has relatively large Stokes shift and high solid-state luminous efficiency; good application potentials are shown in a plurality of fields such as high-performance solid-state light-emitting devices and biological probes.

A fluorescent probe for detecting cytochrome oxidase CYP2D6 and its application, which belong to the field of biomedicine technology. The specific probe substrate can be used to determine the enzyme activity of CYP2D6 in biological systems. The process of CYP2D6 enzyme activity determination is as follows: the benzylmethoxy demethylation reaction in the meso-position pyridinium quaternary ammonium salt of Bodipy compounds is selected as the probe reaction, and the activity of CYP2D6 enzyme in various biological samples is determined by quantitatively detecting the amount of its metabolite generated per unit time. The probe is a selective probe for detecting CYP2D6 activity, which can achieve efficient discovery of CYP2D6 inhibitors (including mechanism-type inhibitors) and in-depth research on CYP2D6-related diseases. The probe can also be used for rapid in vitro screening of reversible and irreversible inhibitors, activators and inducers of CYP2D6, and for detecting drug-drug interactions of CYP2D6 at the in vivo level.

The application provides a method for monitoring lipid oxidation tendency, and belongs to the technical field of fluorescent probe detection. The method utilizes the fluorescence characteristics of a BODIPY molecular probe, and through selecting a molecular probe with a specific structure, directional reaction occurs between the BODIPY molecular probe and oxygen free radicals generated by lipid oxidation at a specific pH value. The conjugated system of the BODIPY molecular probe is destroyed, the half-chlorin structure is broken, and the fluorescence color changes from red to blue-green. According to the change of the fluorescence color before and after the reaction, the visualization monitoring of the lipid oxidation tendency and spatial distribution is realized through fluorescence imaging.

The invention relates to a preparation method of a heavy-atom-free photosensitizer based on a BODIPY dye, the photosensitizer comprises a thiocarbamate light cage, not only can generate singlet oxygen (1O2) under red light irradiation, but also can release carbonyl sulfide (COS), and generates hydrogen sulfide (H2S) under physiological conditions through carbonic anhydrase catalysis. By reasonably adjusting the molecular structure, the singlet oxygen quantum yield and hydrogen sulfide release efficiency of the photosensitizer are remarkably improved. The method is the first case which is reported at present and can simultaneously activate and release the organic optical functional micromolecules of H2S and 1O2 through a single beam of red light. The strategy effectively solves the problem of dependence on oxygen in type II photodynamic therapy (PDT), and further effectively improves the effect of PDT hypoxic tumors. In in-vitro and in-vivo experiments, hydrogen sulfide gas and photodynamic synergic treatment trigger pyroptosis of cells, promote maturation of dendritic cells and stimulate antitumor immune response. Therefore, the invention provides a synergistic treatment strategy combining gas, photodynamic and immunotherapy, and has a remarkable effect in the aspect of hypoxic tumor treatment. The photosensitizer material disclosed by the invention is simple to prepare and low in cost, has a wide application prospect, and particularly provides a solution with huge potential for cancer treatment in the field of biomedicine.

The invention discloses a silver ion fluorescent probe based on pyrrolo-pyrrole aza-BODIPY as well as a preparation method and application of the silver ion fluorescent probe. The probe has a specific chemical structure, and alkynyl groups are modified at two ends of the probe. When the probe is combined with silver ions, the ultraviolet absorption spectrum of the probe has absorption peak widening and red shift within the range of 680-690nm, and meanwhile, the fluorescence emission of the probe under the excitation of 650nm is remarkably quenched, so that the specific dual-mode detection of the silver ions is realized. The probe shows high selectivity and high sensitivity on silver ions, the detection limit can reach the nanomole level, the anti-interference capability is high, and the probe can work in water or various organic solvents. The invention also provides a clear synthetic route of the fluorescent probe. The probe is suitable for rapid and sensitive detection of silver ions in environmental or biological samples.

The present invention discloses a fluorescent probe for detecting cysteine, and its preparation and use methods. The present invention designs a ratiometric fluorescent probe BDP-4SPy based on benzo-BodiPy and 4-mercaptopyridine. The thiopyridine portion of the probe is a good leaving group. It undergoes nucleophilic substitution with the thiol group of Cys to generate BDP-S-Cys, which is then subjected to intramolecular rearrangement to generate BDP-N-Cys, thereby causing a change in the probe fluorescence. The ratiometric fluorescent probe of the benzo-BodiPy dye provided by the present invention has good response performance to cysteine, can achieve sensitive, rapid, and quantitative detection of trace cysteine ​​in a sample, and has the advantages of simple operation, low cost, sensitive response, and easy promotion and application.

The subject invention pertains to compositions of a probe comprising two pH-reversible forms, including the boron dipyrromethene (BODIPY) fluorescent form and the non-fluorescent leuco-BODIPY form, for use in confocal imaging methods of intracellular vesicles as well as extracellular vesicles that are present in conditioned culture medium and / or secreted by living cells, without inducing severe cytotoxicity.

The invention discloses a preparation method and application of a wide-spectrum absorption zinc complex photosensitizer. Through Click reaction, an organic chromophore BODIPY (Bodipy) with a high molar extinction coefficient and a high fluorescence quantum yield is covalently coupled with a dipyrrole zinc (II) complex, and a novel wide-band and strong-absorption zinc complex photosensitizer is successfully constructed. The photosensitizer shows the wide-band absorption characteristic of 300-650 nm, and the capture efficiency of solar energy is remarkably improved. In a photocatalytic COreduction reaction, the complex shows excellent catalytic performance, the CO yield of the complex can reach 18.4 micromoles, and the selectivity exceeds 99%. The invention not only provides an innovative molecular design strategy for developing efficient non-noble metal photosensitizers, but also provides an important scientific reference for realizing solar-driven artificial photosynthesis.

The invention belongs to the technical field of biomedical detection, and relates to a quinoxaline-derived Aza-BODIPY AIE fluorescent probe, the probe is composed of three parts of fluorophore Aza-BODIPY, rotor benzothiazole and lysosome targeted morpholine, the Aza-BODIPY and benzothiazole are connected through a-C = N-C-group, and the probe is a quinoxaline-derived Aza-BODIPY-AIE fluorescent probe. A precursor intermediate probe 1 is formed by connecting a fluorophore Aza-BODIPY and a rotor benzothiazole through a-C-C = N-group. The invention further discloses a synthesis method of the fluorescent probe and application of the fluorescent probe to lysosome viscosity detection. The probe system disclosed by the invention has significantly increased Stokes shift, and effectively avoids overlapping of excitation / emission spectrums; the fluorescent probe shows typical aggregation-induced emission (AIE) characteristics, and fluorescence enhancement is most remarkable under the condition that the water content is 40-50%; the fluorescent probe has wide-range viscosity response capability (2-610cP), 24 times of fluorescence enhancement can be generated at most, and the detection limit is as low as 0.27 cP; the lysosome co-localization performance is excellent, and the cell compatibility is good; the high-contrast real-time imaging monitoring on the viscosity of the living cell lysosome can be realized.

The present invention discloses an organic polymer composite material based on BODIPY light-controlled CO release, as well as its preparation method and application, belonging to the field of biomedicine technology. Tetraaldehyde BDP and 2,2'-bipyridine-5,5'-diamine are dissolved in an organic solvent, ultrasonically dispersed to form a suspension, and after cyclic deoxygenation, heated in a sealed environment for reaction to obtain a powdered black solid BB-COF. BB-COF and pentacarbonyl manganese bromide are suspended in anhydrous ether under a protective atmosphere, and stirred at room temperature for reaction to obtain an organic polymer composite material based on BODIPY light-controlled CO release. The composite material prepared by the present invention has an eggshell-like spherical structure and the ability to release CO under light-controlled CO. It can achieve antibacterial effects through a quadruple synergistic mechanism of photothermal therapy, photodynamic therapy, Fenton reaction, and carbon monoxide (CO) release, making it safer for use in organisms.

This invention belongs to the field of pharmaceutical synthesis technology, and relates to meta-pyridine quaternary ammonium salt-substituted BODIPY compounds, their preparation methods, and pharmaceutical uses, specifically meso-position 3-pyridine quaternary ammonium salt-substituted BODIPY compounds, their preparation methods, and applications. The compounds of this invention, through photosensitization efficiency and in vitro and in vivo antibacterial activity tests, demonstrate good antibacterial activity and can be used to prepare novel pyridine quaternary ammonium salt-containing fluoroboron dipyrrole (BODIPY) photosensitizers, and further to prepare new antibacterial drugs.

A molecular probe for selective detection of a nitrate and / or a decomposition product of a nitrate by generating a fluorescence signal in response to an excitation in the wavelength range between 340-370 and 480-520 nm, wherein the molecular probe is selected from a triarylborane dye according to formula (7) and a BODIPY™ (boron-dipyrromethene) dye according to formula (13):

The invention belongs to the technical field of medicine synthesis, and discloses a pantothenic acid modified aza-BODIPY photosensitizer, the structural general formula of the pantothenic acid modified aza-BODIPY photosensitizer is shown as a formula IV, and n is equal to 1-6; the dashed line represents the presence or absence of a C-C bond. The synthesized pantothenic acid modified aza-BODIPY photosensitizer has good photosensitive activity and cancer cell specific uptake ability, for example, the singlet oxygen yield (phi delta = 0.61-0.69) of photosensitizer compounds 1-8 is higher than that of a reference compound, and the pantothenic acid modified aza-BODIPY photosensitizer can be further prepared into targeted photodynamic drugs for photodynamic therapy of wide non-specific tumors.

The invention provides a single-molecule fluorescent probe based on a BODIPY derivative BP-BNO2 as well as a synthesis method and application of the single-molecule fluorescent probe, and the specific Chinese name of the derivative is 5, 5-difluoro-1, 3, 7, 9-tetramethyl-10-(4-nitrophenyl)-5H-4 lambda 4, 5 lambda 4-dipyrrolo [1, 2-c: 2 ', 1'-f] [1, 3, 2] diazaborane. The invention also provides a synthesis method of the derivative and application of the derivative in detection of hypobromous acid in vitro, cells and nasal cavities of mice with allergic rhinitis. According to the method, on the basis of a derivative BP-BNO2 of BODIPY, the concentration change of hypobromous acid is detected through a fluorospectro photometer in a mixed solution of a phosphate buffer solution and acetonitrile (the volume ratio is 7: 3). The method also realizes noninvasive in-situ imaging at cell and mouse levels, and can be used for in-situ monitoring of hypobromous acid level in the nasal cavity of the mouse with allergic rhinitis and auxiliary diagnosis of allergic rhinitis.