1762 results about "Mitochondrion" patented technology

The specification provides programmable base editors that are capable of introducing a nucleotide change and / or which could alter or modify the nucleotide sequence at a target site in mitochondrial DNA (mtDNA) with high specificity and efficiency. Moreover, the disclosure provides fusion proteins and compositions comprising a programmable DNA binding protein (e.g., a mitoTALE, a mitoZFP, or a CRISPR / Casp) and double-stranded DNA deaminase that is capable of being delivered to the mitochondria and carrying out precise installation of nucleotide changes in the mtDNA. The fusion proteins and compositions are not limited for use with mtDNA, but also may be used for base editing of any double-stranded target DNA.

The invention provides a preparation method of mitochondria-targeted antioxidant hybrid vesicles. The preparation method comprises the following steps: S1, preparing and separating adipose-derived stem cell nano-vesicles; s2, preparing a liposome loaded with dihydromyricetin and distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000-triphenylphosphine targeting peptide by an ethanol injection method; and S3, preparing the hybrid nano vesicles loaded with the dihydromyricetin and the distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000-triphenylphosphine targeting peptide. The invention also provides the hybrid vesicles prepared by the method and application of the hybrid vesicles in preparation of refractory diabetes wound treatment drugs. The lipidosome and the adipose-derived stem cell nano-vesicles are hybridized to prepare the hybridized vesicles capable of targeting mitochondria and resisting oxidation, the hybridized vesicles are applied to wound treatment for the first time, the problem of oxidative stress of diabetic wounds is solved, wound healing is accelerated, and a new strategy is provided for optimizing mitochondrial function defects and oxidative stress of the diabetic wounds.

The invention discloses a ginseng extract rich in rare saponins and preparation and anti-aging application thereof, and belongs to the technical field of oral beauty and skin care products, the ginseng extract is obtained by fermentation treatment of ginseng powder through phytobacterium plantarum, and the preservation number of the phytobacterium plantarum is GDMCC No.64374. The ginseng extract rich in rare saponin is fermented by specific plant lactobacillus, ginsenoside is efficiently converted into CK and F1, various natural plant components such as ginseng polysaccharide are reserved, and a specific synergistic effect is generated. Through verification, the prepared ginseng extract is high in bioavailability, can more effectively inhibit cell inflammation, and can more effectively recover the mitochondrial function of senescent cells and inhibit cell senescence. The preparation method provided by the invention is high in operability and easy to standardize and intellectualize. Compared with a traditional extraction and purification method, the adopted fermentation method is more environmentally friendly and efficient, and large-scale production can be achieved in industrial production.

For use in transcranial photobiomodulation (PBM) or brain wave or heart rate variability (HRV) biofeedback and using event-related potential (ERP) and induced and induced EEG oscillation (ERO) measurements, devices and methods for analyzing and monitoring electroencephalogram (EEG) electrical activity evoked during, prior to, and / or after a neurocognitive test of a human user to stimulate and modulate brain activity. An electroencephalogram (EEG) and photo-biometric modulation (PBM) apparatus in the form of a wearable device with headphones is described for stimulating the brain with PBM or monitoring and evaluating electrical activity generated by the brain of a person during stimulation using biofeedback training and simultaneous occurrence of EEG, also described are methods for testing the cognitive and physiological state of a person during a cognitive test using the provided devices. The PBM light is pulsed to stimulate an increase in target brain activity by providing additional energy to the mitochondria and activating other photosensitive brain processes. PBM stimulation is combined with EEG sensors for detecting EEG responses during cognitive testing using event related potentials (ERPs) and induced and induced EEG oscillations (EROs) for evaluating cognitive status and therapeutic function outcomes of a user.

The invention belongs to the cross technical field of nanomedicine, tumor treatment and immunotherapy, and particularly provides tumor mitochondria-targeted copper death selective induction nanoparticles, which are prepared by the following preparation method: S1, specifically coupling TPY and MHI through condensation reaction to obtain MTPY; s2, MTPY and copper ions are subjected to complexation, and an MTPY-Cu complex is obtained; and S3, the MTPY-Cu complex and albumin are subjected to self-assembly, and the MTPY-Cu-coated Alb nanoparticles are formed. The dosage of copper is only 1 / 1000 of that of free Cu < 2 + >, and equivalent immune regulation and killing effects can be achieved; primary tumors and distant tumor focuses can be inhibited, and lung metastasis is reduced; immune memory can be induced, and relapse can be prevented; the traditional Chinese medicine composition is combined with cis-platinum to remarkably enhance the curative effect and reverse the induced immunosuppression; the invention has the advantages of high biological safety and no obvious liver and kidney toxicity or hemolytic reaction.

The invention relates to the technical field of biological materials, in particular to a hydrogel scaffold material for spinal cord injury repair as well as a preparation method and application of the hydrogel scaffold material. The hydrogel scaffold material AP39 (AP39-coated LIP) for spinal cord injury repair is prepared by combining the liposome-coated AP39 (AP39-coated LIP) serving as a drug with a GelMA hydrogel scaffold, and a scaffold is provided for injured spinal cords, so that axon regeneration of injured parts is guided, mitochondria of the injured parts is targeted to release H2S, neuronal injury is relieved, and the spinal cord injury repair effect is improved. Therefore, nerve regeneration and functional recovery after spinal cord injury are promoted.

The invention relates to an application of a regulator in improving the maturation quality of frozen pig oocytes, the regulator is N-(2-(2-(4-chlorphenyl) thiazole-4-yl) ethyl) butyryl, and the regulator improves the maturation quality of the frozen pig oocytes by improving the mitochondrial function of the frozen pig oocytes after in-vitro maturation. The invention provides a new application of N-(2-(2-(4-chlorphenyl) thiazole-4-yl) ethyl) butyramide, namely the N-(2-(2-(4-chlorphenyl) thiazole-4-yl) ethyl) butyramide is used for developing a novel oocyte vitrification freezing solution and an in-vitro maturation culture solution, and the obtained high-quality oocytes can be beneficial to embryo biotechnology researches such as in-vitro fertilization and somatic cell nuclear transplantation.

The invention discloses an indole-benzothiadiazole derivative as well as a preparation method and application thereof. The general structural formula of the indole-benzothiadiazole derivative provided by the invention is shown in the specification, wherein R is selected from one of methyl piperazinyl, diethylamino, azetidinyl and pyrrolidinyl. The derivative can be specifically combined and fluorescently labeled with mitochondrial RNA in cells, and has good selectivity, high fluorescence signal-to-noise ratio and excellent anti-interference capability in a complex biological system. Meanwhile, the indole-benzothiadiazole derivative provided by the invention is simple in synthetic route, easily available in raw materials, good in stability and convenient to store and use, can be specifically combined with RNA and generate strong fluorescence response, is suitable for real-time detection and imaging of in-vitro and in-vivo mitochondrial RNA, and is an efficient mitochondrial RNA micromolecular fluorescent probe.

The invention discloses a design and preparation method of a cell membrane vesicle preparation loaded with mitochondria. The design method comprises the following steps: loading the mitochondria into an artificially constructed cell membrane bionic vesicle, and constructing a nano-scale vesicle preparation loaded with the mitochondria inside and a cell membrane outside; the preparation method comprises the following steps: preparing cells into a cell suspension; repeatedly extruding the cell suspension through an extrusion device provided with a filter membrane with a specific aperture; and carrying out differential centrifugation on the extruded liquid to prepare the cell membrane vesicle preparation loaded with mitochondria. The invention also discloses the vesicle preparation obtained by the method and application of the vesicle preparation in preparation of drugs for treating mitochondrial damage related diseases. According to the method, rapid and efficient loading of mitochondria in cells can be realized, and the problems of low yield of mitochondria vesicles from natural sources, limited loading efficiency and the like are solved. The prepared vesicle preparation can effectively protect the physiological function of mitochondria, delay rapid inactivation of mitochondria in the using process and achieve efficient mitochondrial transplantation of damaged cells.

The invention belongs to the field of daily chemicals, and provides a composition with a longevity gene activation effect, a product containing the composition and application of the composition, and the composition provided by the invention comprises hexapeptide, chlamydomonas and cornflower extract. The hexapeptide, the chlamydomonas nivalis and the cornflower extract are compounded for use, so that the expression of longevity genes KLOTHO and SIRT1 can be remarkably improved. Once the longevity gene serving as the upstream of a signal regulation pathway is activated, various downstream signal pathways can be regulated, such as inhibition of expression of inflammation-related genes, promotion of DNA repair, telomere homeostasis, gene expression of mitochondrial energy and the like, promotion of synthesis of collagen and the like, and finally, longevity of cells and improvement of skin phenotypes are promoted. The effects of resisting wrinkles, tightening and the like are achieved. Therefore, the composition provided by the invention can activate long-life gene expression and regulate and control multiple signal pathways, has potential effects of improving light aging, tightening and resisting wrinkles and the like, and is an anti-aging composition with great potential.

The invention provides a novel platform for tumor immunotherapy. The platform is formed by self-assembly of full-heavy-chain human ferritin which can be jointly fused and expressed by a polypeptide prodrug mask which can be activated by protease and a mitochondrial disrupting peptide, and can specifically target a tumor microenvironment and induce ICD death of tumors. Meanwhile, an ion channel is formed in a protein shell of the platform, so that immunocompetence metal ions can be loaded in a cavity of the platform, and time-space synchronous anti-tumor immune activation can be carried out in cooperation with ICD. As the mitochondrial disrupting peptide and the metal ions can be optimized according to tumor types, the platform can be used for treating various tumors.

The invention discloses a therapeutic nano-vesicle preparation rich in mitochondrial functional protein, a preparation method of the therapeutic nano-vesicle preparation and application of the therapeutic nano-vesicle preparation in treatment of radioactive skin injury. The preparation method comprises the following steps: culturing, amplifying and cleaning umbilical cord mesenchymal stem cells, dispersing the umbilical cord mesenchymal stem cells in a suspension, mechanically extruding the umbilical cord mesenchymal stem cells into a crude solution through a multistage aperture polycarbonate membrane, and centrifuging, concentrating and purifying the crude solution to obtain the preparation. The preparation has the remarkable advantages that local administration can be realized, inflammation can be quickly inhibited, oxidative stress is reduced, and a repair pathway is activated, so that the preparation is suitable for treating acute radioactive skin injury; the preparation method is easy to standardize and scale, high in stability and convenient for emergency storage and delivery, and avoids the defects of cell therapy; a plurality of targets such as immune inflammation, oxidative stress and the like can be synergistically regulated, the biological effect of hUMSCs is simulated and partially replaced, and the limitation caused by the cell survival rate and the microenvironment dependency is avoided; the key function of hUMSCs is covered in function, and the clinical feasibility, safety and emergency suitability of the application level are higher.

The invention discloses a method for identifying key mitochondrial autophagy and ferroptosis genes in sepsis-induced ARDS and an immunoregulation mechanism of the key mitochondrial autophagy and ferroptosis genes. The method comprises the following steps: screening differential expression genes DEGs related to the sepsis-induced ARDS from a GEO gene expression database; and screening the DEGs by using LASSO and SVM + RFE, and identifying 12 key DEGs. The diagnostic potential of the genes in sepsis-induced ARDS is verified through an ROC curve, and the effect of the genes in immune response is disclosed through gene enrichment analysis and immune infiltration analysis. According to the invention, a key gene FTH1 is identified, a miRNA gene [miR-224-5p] targeting the FTH1 and a pathologic process in which the miRNA gene [miR-224-5p] participates in regulation and control of sepsis-induced ARDS are found, and a close relationship between the FTH1 and E3 ubiquitin ligase SMURF1 is found.

The invention discloses a super-resolution image mitochondrial subtype-based cell classification and analysis device and method, and the method comprises the steps: 1, calculating the characteristics of each mitochondrial according to all mitochondrial segmentation images in cells, carrying out the dimension reduction processing, carrying out the clustering of the mitochondrial characteristics after the dimension reduction, and obtaining the type of each mitochondrial; and step 2, calculating the proportion of each type of mitochondria in the cells according to the cells and the types of the mitochondria obtained in the step 1, determining the characteristics of the cells, performing dimension reduction processing, and clustering the characteristics of the cells after dimension reduction to obtain the types of the cells. According to the method, the form and position parameters of the single mitochondria can be efficiently represented, and favorable conditions are provided for quantifying the cell state.

The invention provides a preparation method of a cordyceps militaris fermentation product with anti-aging, mitochondrial function enhancing, anti-oxidation and autophagy promoting effects, and the preparation method comprises the following steps: inoculating cordyceps militaris into a fermentation substrate for liquid fermentation culture to obtain a fermentation mycelium; the fermented mycelium is subjected to wall breaking or centrifugal separation, concentration and freeze-drying, and the cordyceps militaris fermented product is prepared; wherein the fermentation substrate comprises oat bran and / or lily. The cordyceps militaris fermentation product prepared by the method shows remarkable anti-aging, anti-oxidation, mitochondrial function enhancing and autophagy promoting effects in in-vitro cell experiments and in-vivo animal models, the process period is short, the preparation process is simple and easy, liquid fermentation in the whole process is suitable for large-scale production, and the preparation cost is low.

The present invention relates to mitochondrial-targeted agents and their utility in increasing telomere length and / or suppressing telomere shortening.

The invention relates to the technical field of biomedical materials, in particular to a mitochondrial transplantation treatment system and application thereof. The mitochondrial transplantation treatment system provided by the invention comprises mitochondria obtained by separation and a transfer vector. Wherein the mitochondria transplantation treatment system is obtained by assembling the mitochondria and the transfer vector, and the transfer vector comprises at least one of artificial lipid vesicles, vesicles derived from cell membranes and exosomes. The preparation process of the mitochondrial transplantation treatment system is simple, healthy mitochondria can be efficiently and rapidly transferred into cells or tissues at the same time, and the mitochondrial function of damaged mitochondrial cells is repaired. The mitochondrial transplantation treatment system has the potential of treating related diseases caused by mitochondrial dysfunction.

The invention discloses development and application of a molecular marker for identifying a female parent of a pine tree. The technical problem to be solved is how to identify or assist in identifying the female parent of the pine tree. Specifically, the invention discloses application of substances for detecting SNP40, SNP81, SNP318, SNP443, SNP521 and / or SNP590 in identifying a female parent of a pine to be detected or preparing a product for identifying the female parent of the pine to be detected. The SNP40 is an SNP in pine mitochondria, is the 40th nucleotide of a sequence 1, and is C or T; the SNP81 is an SNP in pine mitochondria, is the 81st nucleotide of a sequence 1, and is A or C; the SNP254 is an SNP in pine mitochondria, is the 254th nucleotide of a sequence 1, and is C or T; the SNP318 is an SNP in pine mitochondria, is the 318th nucleotide of a sequence 1, and is A or C; the SNP443 is an SNP in pine mitochondria, is the 443rd nucleotide of a sequence 1, and is G or T; the SNP521 is an SNP in pine mitochondria, is the 521th nucleotide of a sequence 1, and is G or T; the SNP590 is an SNP in pine mitochondria, is a 590th nucleotide of a sequence 1, and is A or C. The source of a female parent to be detected is determined according to the sequence, and the invention can be used for industrial and agricultural production.

The invention relates to application of a heterocyclic compound as a fluorescent probe. The structural formula of the heterocyclic compound is shown as a general formula I, and R1, R2, R3, R4, R5, R6, R7 and R8 are independently one of hydrogen, alkyl, hydroxyl, phenyl, hydroxyphenyl, indole and oxymethyl. According to the present invention, the heterocyclic compounds represented by the general formula I can be specifically positioned in mitochondria, and have long fluorescence lifetime, such that the heterocyclic compounds are suitable for being used as mitochondria fluorescence probes.

The invention provides a polypeptide for inducing tumor cell immunogenic death, a bioactive solution and application of the polypeptide and the bioactive solution, and belongs to the technical field of biological medicine. Comprising an end-capping group, a phosphorylated gelling factor, a matrix metalloproteinase-2 response sequence and a transient receptor potential vanillic acid receptor 1 (TRPV1) agonist which are connected in sequence, the amino acid sequence of the phosphorylated gelling factor is GFFpY, and the response sequence of the matrix metalloproteinase-2 is PLGVR. The anti-tumor polypeptide provided by the invention can generate enzymatic in-situ self-assembly through the action of ALP and MMP-2 overexpressed outside tumor cells, and sequentially release pharmacophores outside and inside the cells to cause mitochondrial stress and endoplasmic reticulum stress and synergistically induce immunogenic death of the tumor cells.

The invention belongs to the technical field of biological medicine, discloses application of a natural protein molecule in preparation of a medicine for resisting Parkinson's syndrome, and particularly discloses a protein molecule which comprises A-B, A comprises A-chain polypeptide or functional variants of the A-chain polypeptide, and B comprises A-chain polypeptide or functional variants of the A-chain polypeptide. B comprises a B-chain polypeptide or a functional variant thereof; the A is in disulfide bridging with the B; the amino acid sequence of the A-chain polypeptide is as shown in SEQ ID NO: 1; the amino acid sequence of the B-chain polypeptide is as shown in SEQ ID NO: 2. The invention discloses a natural protein molecule and application thereof in treatment and / or prevention of Parkinson's syndrome for the first time, the protein molecule can show antioxidant capacity by activating GSH in cells and inhibiting ROS formation, and meanwhile, the protein molecule can also show antioxidant capacity by activating mitochondrial activity and anti-GFAP activity in an MPP-induced brain injury environment, so that the protein molecule can be used for treating and / or preventing Parkinson's syndrome. The natural protein molecule has the medical effect of relieving and treating Parkinson's syndrome.

The invention discloses a rice large-scale metabolism regulator DGP1 and crop metabolism quality design. The invention finds that the structural domain of the rice DGP1 family protein TIGR01589 targets a plurality of metabolic enzymes and promotes the metabolic enzymes to be transferred to a cell nucleus; the structural domain also has an intervention effect on a light breathing pathway by changing the composition of a glycine decarboxylase compound in mitochondria. Based on a molecular mechanism of DGP1, a chimeric protein ST containing a conservative structural domain and coupled with a chloroplast positioning signal is designed, and genetic engineering modification of pakchoi is realized. The modification strategy not only can guide substrate flow of a central glycolysis pathway to flavonoid biosynthesis, but also can promote accumulation of saccharides derived from photosynthetic products in leaves.

The invention relates to the field of nano drug delivery systems, in particular to composite hydrogel targeting macrophage mitochondria as well as a preparation method and application of the composite hydrogel. The ROS response type liposome composite injectable hydrogel targeting the macrophage mitochondria is prepared by compounding the cationic liposome and the hydrogel, can be used as a nano drug delivery system suitable for oral local drug delivery, has good biocompatibility, and can target the mitochondria. The composite hydrogel is easy to prepare, easy to administrate and high in viscosity, has the characteristics of continuous drug release mode, minimum dosage frequency, low drug toxicity and the like, and is beneficial to being widely applied to treatment of periodontitis related diseases.

The present disclosure relates to compositions and methods for cryopreservation of mitochondria, cryopreserved compositions comprising mitochondria, and therapeutic uses thereof.

The invention relates to the technical field of biological medicines, in particular to a preparation method of extracellular vesicles derived from myocardial cells derived from human induced pluripotent stem cells as well as a product and application of the extracellular vesicles. The method comprises the following steps: pretreating human induced pluripotent stem cell derived myocardial cells (hiPSCs-CMs) by using honokiol, and culturing the treated human induced pluripotent stem cell derived myocardial cells to collect extracellular vesicles (EVs) secreted by the human induced pluripotent stem cell derived myocardial cells. The extracellular vesicles secreted by the myocardial cells derived from the human induced pluripotent stem cells pretreated by honokiol are rich in functional mitochondria, show a better myocardial infarction treatment effect in animal experiments, and have huge clinical potential.

The invention discloses preparation and application of a novel diagnostic kit for a mitochondrial network regeneration function evaluation test, and in the invention, combined detection of related proteins DRP1, MFN2, OPA1 and TIM23 is carried out by utilizing four mitochondrial networks, so that the mitochondrial regeneration function state of sample cells is judged. The kit disclosed by the invention is used for detecting samples such as human blood, urine, cell cultures and tissues by utilizing an ELISA (Enzyme-Linked Immunosorbent Assay) method. Results show that the kit can accurately detect the content of four proteins so as to judge the function of sample mitochondria. The mitochondrial network regeneration capacity function evaluation kit based on a double-antibody sandwich method in an ELISA method can conveniently and accurately evaluate the mitochondrial network regeneration capacity in a human body specimen. The invention discloses preparation and application of the novel diagnostic kit for evaluating the regeneration function of the mitochondrial network. The diagnostic kit plays an auxiliary role in mitochondrial detection in scientific research and disease diagnosis and treatment. The method is applied to mitochondrial function evaluation in all diseases with abnormal cell mitochondrial functions.

The invention relates to the technical field of medical chemistry, in particular to a bridge oxygen site regulatively modified rhodamine anti-cancer fluorescent compound as well as a preparation method and application thereof. The invention develops the rhodamine anti-cancer fluorescent compound of which the meso site is functionalized by tetraarylethene and the bridge oxygen site can be regulated and modified, the fluorescent compound has adjustable red light-near infrared fluorescence emission and excellent fluorescence quantum yield, and the quantum yield can be as high as 99%; the fluorescent compound specifically targets mitochondria, and mainly inhibits growth of tumor cells by inducing para-apoptosis; under irradiation of 655 nm near-infrared laser, ferroptosis is further triggered, efficient treatment of tumor cells is achieved through chemical-photodynamic synergistic effect, and a new treatment thought is provided for overcoming tumor drug resistance.

The invention discloses a composition, a kit and a method for detecting a mitochondrial gene of sea eel. A primer probe composition targeting the D-LOOP gene of the sea eel mitochondria is designed, and the nucleotide sequences of the primer probe composition correspond to SEQ ID No.1-3. On the basis, the invention further constructs and optimizes a specific real-time fluorescent PCR detection method for the D-LOOP gene of the sea eel mitochondria. According to the method, a part of sequence of the D-LOOP gene of the mitochondria of the sea eel is taken as a target region, a primer and a TaqMan-MGB probe are designed in a targeted manner, and finally a set of rapid, sensitive and high-specificity detection system is established. The detection system can be effectively applied to adulteration and germplasm identification work of the sea eel, and has a very wide industrial application prospect.

The invention discloses a medicine prepared from apoptotic fibroblast-derived vesicles and used for preventing and treating myocardial ischemia-reperfusion injury as well as a preparation method and application of the medicine, and relates to the technical field of biological medicines. The method comprises the following steps: digesting an in-vitro animal heart apex tissue to obtain a digested cell suspension; the myocardial fibroblasts are obtained; the method comprises the following steps: culturing myocardial fibroblasts in a DMEM (dulbecco's modified eagle medium) solution through apoptosis inducer H2O2 to obtain a culture solution; according to the method, apoptosis of fibroblasts is induced to generate specific extracellular vesicles, the surface of the extracellular vesicles carries CD22 as a marker, the content of the extracellular vesicles is enriched in miR-1246 and is taken by myocardial cells, expression of p53 is down-regulated, mitochondrial homeostasis is maintained, and the extracellular vesicles can be used for detecting apoptosis of the fibroblasts. The myocardial cell apoptosis is inhibited.

Platelet-derived extracellular vesicles (PEVs) containing mitochondria are used in treating ocular disorders or symptoms of ocular disorders. The PEVs are collected by obtaining blood from one or more donors, adding an anticoagulant and a buffer to the blood to form a mixture, separating the mixture into a supernatant and platelet-rich plasma (PRP), collecting the PRP, stimulating the collected PRP to displace the extracellular vesicles from the platelets in the PRP, and collecting the extracellular vesicles as PEVs.