142 results about "Mitochondrion" patented technology

The application relates to a primer probe composition, a kit and a detection method for specifically detecting Blattella germanica. The specific primer and TaqMan probe are designed and screened based on the specific sequence of the mitochondrial COI gene of Blattella germanica. The detection method has extremely high specificity, has no cross reaction with close species such as Periplaneta americana and Blattella germanica, has high sensitivity, the minimum detection limit is 1 fg / muL, and has good repeatability, with the variation coefficients of groups and between groups being less than 3%.

The application discloses a new use of Leonurine or a pharmaceutically acceptable salt thereof in the preparation of a medicament for treating radiation-induced brain injury, specifically, providing a safe and effective therapeutic drug for iron death, neuroinflammation, neuron damage and lipid metabolism disorder accompanying the radiation-induced brain injury, solving the technical problem of lack of specific treatment means in the prior art, and having a wide clinical application prospect. Experiments adopt a C57BL / c mouse 30 Gy whole brain X-ray radiation model, and results show that Leonurine can significantly improve the body weight decrease and survival rate of the model mice, reduce the serum IL-6 and IFN-alpha levels, inhibit the activation of microglial cells in the cerebral cortex and hippocampus, protect and repair damaged neurons, and improve the ultrastructure of mitochondria and synapses; meanwhile, the expression of GPX4 and SLC7A11 proteins in the brain tissue is up-regulated, the GSH content is increased to inhibit iron death, and the levels of GPE, GPS and GPC related to lipid metabolism are restored.

The present invention relates to a medium composition for in vitro fertilization and / or in vitro culture comprising a compound represented by chemical formula 1 or a pharmaceutically acceptable salt thereof, and a method using same. The compound or composition suppresses reactive oxygen species and lipid peroxidation in ovarian granulosa cells to preventing cytotoxicity and mitochondrial dysfunction caused by apoptosis and ferroptosis, leading to an improvement in the quality of aged oocytes, and to increase the developmental rate and blastocyst formation rate of pre-implantation embryos, thereby improving the efficiency of in vitro fertilization. In addition, treatment with the medium composition during the vitrification process of in vitro–fertilized embryos improves re-expansion and survival rates, thereby reducing structural and metabolic damage. Accordingly, the present invention can be advantageously applied to an assisted reproductive technology for treating infertility and subfertility, and in vitro fertilization and / or in vitro culture for improving the propagation efficiency of livestock.

The present invention comprises (i) or (ii). (i) Golden oyster mushroom or a processed product thereof. (ii) Nicotinamide mononucleotide and 5-deazaflavin.

Provided is a use of mitofusin 2 (MFN2), an MFN2 variant capable of interacting with SERCA2, or an MFN2 expression promoter in maintaining and / or promoting tumor-killing capability and / or viability of a CD8 T cell. Also provided is a use of a CD8 T cell overexpressing MFN2 or a variant thereof for treatment of cancer.

The application belongs to the technical field of biological medicine, and particularly relates to an anti-aging ergothioneine composition for improving mitochondrial function and a preparation method thereof. The composition comprises the following components: ergothioneine, sea hao polysaccharide and entada extract. The ergothioneine has good mitochondrial targeted antioxidant activity, can up-regulate the expression of Sirt1 to activate PGC-1a, and promote mitochondrial biosynthesis; the sea hao polysaccharide is helpful to restore mitochondrial function, promote the generation of mitochondrial high-energy phosphoric compounds, improve cell activity and energy supply for regeneration, and can up-regulate the expression of key genes for mitochondrial biosynthesis and ATP synthase related genes. The entada extract can synergize with the ergothioneine and the sea hao polysaccharide, significantly down-regulate the expression of aging related proteins such as p53, p21 and p16 in cells, simultaneously inhibit the transcription of aging related genes, and has a good protective effect on aging cells.

The application relates to the technical field of biological materials, and mainly relates to a cell mitochondrial organic fluorescent probe and a preparation method thereof. The cell mitochondrial organic fluorescent probe comprises the following structural formula: The cell mitochondrial organic fluorescent probe provided by the application has the effect of targeting cell mitochondria, accurate positioning, good biocompatibility, AIE characteristics and low cytotoxicity, does not need washing operation, can avoid water washing, makes the dyeing process more convenient and economical, is an excellent mitochondrial imaging material, and can effectively realize rapid targeted positioning and imaging of mitochondria.

The application discloses a kind of SNX9 engineering mitochondria vesicles and its preparation method and application in improving insulin resistance.The application first constructs the vector of overexpression SNX9 gene, transfects cell, obtains stable overexpression SNX9 gene cell strain, culture, collects culture solution, obtains SNX9-MDVs by centrifugal separation;Then mtDNA is extracted from cell and introduced into SNX9-MDVs, to obtain the engineering mitochondria vesicles mtDNA-SNX9-MDVs rich in mtDNA.MtDNA-SNX9-MDVs are used for insulin resistance treatment, and the results show that mtDNA-SNX9-MDVs can effectively increase mitochondrial ATP and mtDNA content, enhance cell antioxidant enzyme activity, reduce ROS, improve mitochondrial network structure, increase the expression of insulin signal pathway related proteins, and show obvious mitochondrial function repair potential and insulin resistance improvement effect.

The application belongs to the technical field of biological medicine and medical cosmetology, and particularly relates to a kind of composition for mitochondrial protection type cosmetology and skin repair based on hydrogel and application thereof, the composition comprises a mitochondrial protection system and a synergistic active ingredient, the mitochondrial protection system comprises a hydrogel and a mitochondrial protection solution, the hydrogel comprises a synthetic polymer, and the mitochondrial protection solution comprises 200-300 mM mannitol, 0.2-5 mM ATP and 0-5% w / v trehalose; the synergistic active ingredient comprises a mixture of one or more of 0.01-0.1% ascorbyl palmitate, 0.1-1 mM glutathione and 0.5-2% nicotinamide. The composition is applied to microneedle repair, ice needle patch preparation and mask preparation and other aspects; through microneedle repair, ice needle patch or mask and other forms, effective delivery of mitochondria is realized, all-round care and repair are provided for the skin, and the delivery capacity is improved.

The application relates to the field of nanobiomedical technology, and particularly relates to application of polyethylene glycol modified black phosphorus nanosheets in prevention and treatment of acute liver injury. The polyethylene glycol modified black phosphorus nanosheets not only have a significant active oxygen scavenging capacity, but also have calcium ion chelation and buffering capacity, can simultaneously regulate intracellular oxidation-reduction homeostasis and calcium homeostasis, inhibit intracellular calcium overload, especially mitochondrial calcium overload, maintain mitochondrial function, and further reduce liver cell damage, apoptosis and inflammatory response, and play a liver protection role. The application finds that the black phosphorus nanosheets are used in calcium ion chelation and inhibition of cell calcium overload, and breaks through the limitation that the black phosphorus nanosheets are mainly used as active oxygen scavenging materials in the prior art. The material can also adsorb copper ions and realize radioactivity copper labeling without chelating agent, and can be used for liver enrichment tracing and integrated diagnosis and treatment application. The material has a significant protective effect on paracetamol-induced acute liver injury.

This invention discloses an antibody-engineered bone marrow stromal cell and its applications, belonging to the field of biomedical technology. Using bone marrow stromal cells as a biological carrier, this invention externally modifies them with immune checkpoint antibodies to form an antibody-coated engineered cell preparation, thereby achieving targeted and precise mitochondrial delivery of exhausted T cells. This cell preparation can be used in combination with other immunotherapies that can induce T cell exhaustion, such as tumor immunotherapies and CAR-T therapy, to help enhance their anti-tumor efficacy and duration of effect.

The application provides a digital PCR kit for detecting highland invasive species red-eared slider and bullfrog, a set of red-eared slider specific primer probes, which are composed of a primer pair for a specific segment of a red-eared slider mitochondrial CYTB gene and a red-eared slider specific TaqMan probe matched therewith; a set of bullfrog specific primer probes, which are composed of a primer pair for a specific segment of a bullfrog mitochondrial 12S rRNA gene and a bullfrog specific TaqMan probe matched therewith, and a set of internal standard (IPC) primer pairs and internal standard TaqMan probes for monitoring the nucleic acid extraction and amplification reaction process monitoring. The application is provided with the primer pair for the specific segment of the red-eared slider mitochondrial CYTB gene and the red-eared slider specific TaqMan probe, the primer pair for the specific segment of the bullfrog mitochondrial 12S rRNA gene and the bullfrog specific TaqMan probe, and a common internal standard to construct a triple detection reaction system, thereby avoiding the quantitative uncertainty caused by the single species detection in the prior art which needs to be carried out in multiple reactions or relies on a standard curve for relative quantification.

The present application belongs to the technical field of coordination chemistry and biomedical science, and provides a multifunctional iridium (III) complex, a preparation method and application thereof. The multifunctional iridium (III) complex (Mito-Ir) is composed of an iridium (III) complex cation and a coordination anion shown in the following formula. Mito-Ir can efficiently target mitochondria, and also has the abilities of phosphorescence imaging, type I and type II active oxygen generation, and photocatalytic oxidation of nicotinamide adenine dinucleotide. Under light irradiation, Mito-Ir triggers severe mitochondrial dysfunction through the above synergistic effect, and then specifically activates the caspase-3 / GSDME signaling pathway, and significantly induces pyroptosis; this pyroptosis-based cell death mechanism can effectively overcome the apoptosis tolerance of tumor cells, and is accompanied by the release of a large amount of inflammatory factors and damage-associated molecular patterns, stimulates immunogenic cell death, and activates the body's anti-tumor immune response.

This invention discloses a layered anti-aging composition and its application. It comprises 4-10 parts of *Vigna angularis* fruit extract, 0.01-0.2 parts of soluble collagen, and 10-20 parts of *Fucus vesiculosus* extract, all parts by weight. The composition of this invention employs a layered anti-aging skincare strategy ("layered" generally refers to different skin areas, i.e., "cellular layer-dermis-epidermal layer"). This composition can promote the expression of LOR (lower retinal occlusion) and the relative expression level of TGM5 (epidermis), increase the content of type I procollagen C-terminal peptide and elastin (dermis), enhance mitochondrial activity, and increase ATP content (cellular layer). That is, it works progressively from the epidermis to the dermis to the cellular layer, having a relevant effect on each layer of skin, and possessing the functions of enhancing skin elasticity, firming, anti-aging, and repairing the skin barrier.

This invention provides biomimetic nanopharmaceuticals for mitochondrial dysfunction, methods for producing the same, and applications. [Solution] The biomimetic nanodrug comprises an RGD-engineered exosome as a carrier, decalinium chloride modified on the surface of the carrier, and oxaliplatin encapsulated inside the carrier. The biomimetic nanodrug of the present invention (OXA@Exo-RD) protects cargoes that induce mitochondrial dysfunction in the blood circulation process, sequentially increasing the accumulation of mitochondria in the cargoes by targeting them. Cargo 1 (DQA) induces oxidative stress, triggering mitochondrial-mediated apoptosis and mitochondrial dysfunction, while cargo 2 (OXA) disrupts mitochondrial DNA and inhibits the initiation of DNA repair, thereby improving chemotherapy resistance. Both cargoes synergistically overcome CRC drug resistance and inhibit its migration.

This invention discloses a specific molecular marker for identifying eggplant Saet CMS cytoplasmic male sterility lines and its application. The molecular marker, Saet218b, was selected by comparing the mitochondrial genomes of the eggplant Saet CMS cytoplasmic male sterility line and its maintainer line. Through bioinformatics analysis, the candidate specific gene for Saet CMS male sterility, orf218b, was obtained, and its nucleotide sequence is shown in SEQ ID NO: 1. Based on this sequence information, the molecular marker Saet218b for identifying eggplant Saet CMS cytoplasmic male sterility lines was successfully designed. The molecular marker of this invention can be used to identify eggplant Saet CMS cytoplasmic male sterility lines efficiently and with a simple procedure. The results can be used for marker-assisted breeding of eggplant Saet-derived cytoplasmic male sterility lines, improving breeding efficiency. It also provides technical support and theoretical basis for the identification of eggplant specific cytoplasmic germplasm.

This invention discloses a method for preparing fucoidan from *Sargassum hemifolia* and its application in the preparation of anti-hepatocellular carcinoma drugs. Experimental results show that *Sargassum hemifolia* fucoidan Fb has a strong inhibitory effect on HepG2 hepatocellular carcinoma cells, significantly stronger than the inhibitory effects of *Sargassum henryi* polysaccharide Fh and *Sargassum fusiforme* polysaccharide SFPS on HepG2 hepatocellular carcinoma cells. After treatment of HepG2 hepatocellular carcinoma cells with *Sargassum hemifolia* fucoidan DF1 and DF2, apoptosis is ultimately induced through the interaction and cooperation of exogenous (death receptor pathway) and endogenous (mitochondrial pathway). *Sargassum hemifolia* fucoidan possesses strong antitumor activity and therefore can be used to prepare anti-hepatocellular carcinoma drugs.

This invention discloses a tandem-responsive self-assembled molecular probe and its applications. The HPO-CYFF-TPP of this invention is converted to HO-CYFF-TPP under the dephosphorylation of overexpressed ALP, restoring the intramolecular charge transfer effect. It then self-assembles into nanoparticles in situ through π-π stacking, exhibiting fluorescence signal quenching and photoacoustic signal enhancement at low viscosity. Upon laser irradiation, the NPs generate a large amount of singlet oxygen in the mitochondria, thereby achieving efficient photodynamic therapy (PDT) and leading to increased viscosity. At high viscosity, due to the suppression of the distorted intramolecular charge transfer effect of HO-CYFF-TPP, the NPs activate the FL signal with a high signal-to-noise ratio and further enhance the PA signal. Therefore, the HPO-CYFF-TPP of this invention can achieve efficient PDT and enable real-time therapeutic effect assessment through FL / PA signals.

The present invention relates to the cosmetic use of an active ingredient obtained from the species Myrtus communis, as a complexion perfector, by acting on mitochondrial activity. This active ingredient comprises an extract of Myrtus communis, the latter comprising at least 20% carbohydrates and less than 10% polyphenols in the total dry matter of the extract.

A collar worn around the neck of an animal delivers non-invasive light energy to free-floating mitochondria flowing through the animal's veins and arteries. The collar comprises a housing which holds at least one light source, a power source, a controller and in some configurations a reflector. The housing has an aperture through which the light energy is emitted towards the animal's tissue. The housing is connected to a band or harness, which positions the housing where the neck broadens out to the shoulder. Preferably the collar is secured around the neck of the animal loosely enough that the housing falls naturally by gravity to where the neck broadens out to the shoulder. The non-invasive light energy impinges the animal's fur or tissue and penetrates to free- floating mitochondria in the blood flowing through the animal's veins and arteries. This results in improved oxidative phosphorylation and mitochondrial function in the treated mitochondria.

The present invention relates to a method for obtaining a functionally enhanced mitochondria and a use thereof, and specifically, to: a mitochondria isolated from cells overexpressing glutathione synthase; and a use thereof for preventing, alleviating, or treating muscle weakness-related diseases.

The present invention provides novel mitochondria-targeted Atovaquone compounds (Mito-ATO), a mitochondria-targeted derivative of Atovaquone, and methods of using such compounds. Methods of treating cancer using mito-ATO are also provided. Methods of enhancing an anti-tumor immune response by administering mito-ATO are further provided.

The application discloses a kind of short peptides simulating RhoE C end, derivative and its pharmaceutical use in treating ventricular remodeling, belong to biological medicine technical field.The short peptide and derivative contain RhoE C end 200-240 linear function domain, can be combined and activated WWP2, remove HGS self-inhibition, start endosome-lysosome degradation pathway.Short peptide sequence is as SEQ ID NO.1, derivative can be coupled with TAT, T7 or RVG membrane penetrating peptide (SEQ ID NO.2-4), and endocytosed to myocardial cell by caveolin high efficiency delivery.The application solves the defects that existing anti-ventricular remodeling drug inhibits protective autophagy flow, and the ability of eliminating toxic protein is limited, can efficiently eliminate damaged mitochondria and misfolded protein, inhibits myocardial hypertrophy, fibrosis, improves cardiac remodeling, provides safe and effective treatment strategy for related diseases.

This invention discloses a Polygonatum sibiricum vesicle preparation for treating premature ovarian failure, its preparation method, and its application. The preparation method includes: cleaning Polygonatum sibiricum, juicing, filtering to remove residue, and collecting the juice; subjecting the juice to gradient centrifugation to remove residual plant tissue, plant cells, and plant cell debris; then using an extraction reagent for stepwise precipitation, collecting the precipitate, and resuspending it to obtain the final product. The Polygonatum sibiricum vesicle preparation prepared by this invention can be efficiently taken up by ovarian granulosa cells. It effectively treats premature ovarian failure by activating the Nrf2 signaling pathway, upregulating antioxidant enzyme expression, scavenging reactive oxygen species, restoring mitochondrial membrane potential, inhibiting apoptosis, and restoring the expression levels of estrogen, follicle-stimulating hormone, and anti-Müllerian hormone. The preparation method of this invention is standardized, biocompatible, and safe, and has broad clinical application prospects.

This paper provides a method for evaluating oxidative stress levels using mitochondrial oxidative stress markers. [Solution] A method for evaluating the level of oxidative stress according to the embodiment is 2-methylthioadenosine (ms) in mitochondria. 2 A) A process to quantify, and ms 2 The process includes a step in which the quantitative value of A is associated with the level of oxidative stress.

The application provides a composition for improving mitochondrial function and application thereof, and relates to the technical field of hyaluronic acid grafts.The application first finds that hyaluronic acid oligosaccharide-cyclopentapeptide grafts obtained by covalent combination of hyaluronic acid oligosaccharides and cyclopentapeptides which do not have the effect of improving mitochondrial function alone can effectively improve mitochondria or restore mitochondrial function and cell function, thereby providing a new functional ingredient for developing mitochondrial function regulators, cell function regulators, and products for improving the ability of cells to resist aging.Further, the application also finds that the hyaluronic acid oligosaccharide-cyclopentapeptide grafts combined with ergothioneine can produce a synergistic effect, thereby bringing beneficial effects for further improving mitochondrial function, cell function, and the ability of cells to resist aging.

This invention discloses a method for studying the mechanism by which the mucoprotein channel 1 (TRPML1) regulates the outflow of zinc ions from lysosomes and induces changes in mitochondrial ATP. This method uses 13 nm AuNPs as loading materials to construct a logic-gated sensor for pH and zinc ion regulation within lysosomes and for TRPML1 mRNA in the cytoplasm. It also uses 5 nm AuNPs as loading materials to construct a sensor targeting mitochondrial ATP for cell imaging. This method enables the detection of mRNA and zinc ions in the cytoplasm, lysosomes, and mitochondria. 2+ ATP-associated ATP sensors, Zn 2+ Three sensors—a sensor, an mRNA sensor, and an X-ray sensor—are used simultaneously to image cells, aiming to answer questions about the mechanisms of ATP changes in mitochondria under the regulation of zinc ion efflux from lysosomes by TRPML1.

This invention discloses a method for constructing carrier-free NADH nanoparticles to restore the function of aging hepatocytes, the product, and its applications, belonging to the pharmaceutical field. The method includes preparing carrier-free nano-coordination polymer NADH-Gd using a reverse microemulsion method, followed by a two-step lipid modification process: first, DOPA modification, then DPPC, cholesterol, and DSPE-PEG. 5k NADH-PEG was synthesized by modification, with DSPE-PEG added during the modification process. 5k NADH-Gal is ultimately synthesized from NADH-Gal. NADH-Gal exhibits excellent hepatocyte targeting and can replenish NAD+ in senescent hepatocytes in the MASH environment. + The content is increased by adjusting... Aldh18a1 The expression of [a specific substance] regulates proline metabolism. Improved proline metabolism can restore mitochondrial function by promoting mitophagy and mitochondrial biosynthesis, thereby alleviating hepatocyte aging. Simultaneously, it reduces monocyte recruitment and hepatic stellate cells (HSCs), ultimately providing an effective treatment for MASH. This invention provides a novel strategy for the treatment of MASH.