The invention belongs to the technical field of
natural product extraction and preparation, and particularly relates to a method for ultrasonic compound
enzyme assisted extraction of North America crabapple
polysaccharide. According to the method, North America crabapple is taken as a
raw material, and the North America crabapple
polysaccharide is extracted and prepared by optimizing
process conditions of an ultrasonic compound
enzyme-assisted extraction method by utilizing a response surface method. Compared with a conventional
hot water extraction method, the
polysaccharide extraction rate is remarkably improved. An
infrared spectrum and a
scanning electron microscope are used for determining the structure of the
malus prunifolia fruit polysaccharide, and the result shows that the
malus prunifolia fruit polysaccharide contains a pyranoid ring, a beta-glucosidic bond and an alpha-glucosidic bond which are related to various biological
metabolism. And finally, determining
superoxide anion removal capacity and
cholesterol adsorption capacity of the North American
malus prunifolia fruit polysaccharide to find that the North American malus prunifolia fruit polysaccharide has good
antioxidant activity and blood fat reducing activity. Therefore, the North America plum-leaf crab polysaccharide can be widely applied to the fields of functional foods, nutritional supplements, natural
plant antioxidants and the like in the future, so that the comprehensive utilization value of the North America plum-leaf crab is effectively improved.