The invention discloses a thickened oil cold-water-injected fat-soluble dissolving nano oil-displacing agent HCD that comprises the following components in percentage by weight: 10-50% of ester compound, 5-30% of alcohol compound, 0-20% of amine compound or ether compound, 0-35% of sodium salt or ammonium salt, 0-20% of wetting agent, 0-20% of aldehyde compound, 0-20% of biosurfactant and 0-20% ofacidic substance. The invention further discloses a preparation method of the HCD and application of the HCD in oil field exploitation and rapid pipeline petroleum transportation. The HCD prepared bythe invention has the characteristics of strong fat solubility, water solubility, viscosity reduction, through-seam throat expansion, weak oil wetness, low tension, high oil displacement efficiency,safety and environmental protection; according to the invention, the oil displacing agent enters the nanometer gaps of the thickened oil; paraffin, asphalt and colloid are dissolved in a non-polar fat-soluble manner, the thickened oil is thinned by rapid viscosity reduction, the production is easy, the pipeline transportation is easy, and the low-cost and high-benefit large-scale industrial production of naphthenic thickened oil, super thickened oil and other thickened oil is realized.