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Corn SOD molecular modifying agent and modifying process thereof

A technology of modifiers and molecules, applied in biochemical equipment and methods, enzymes, enzyme stability, etc., can solve the problems of poor covering effect, small modifier molecules, reducing antigenicity and maintaining the activity of modified enzymes, etc.

Inactive Publication Date: 2006-06-28
SHENYANG AGRI UNIV
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AI Technical Summary

Problems solved by technology

Small molecule modifiers are far inferior to macromolecular modifiers in terms of reducing antigenicity and maintaining the activity of modifying enzymes. The reason may be that although the amino groups related to the antigenic determinant are modified, the modifier molecule is small and the covering effect is poor. effectively destroyed

Method used

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Experimental program
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Embodiment Construction

[0020] 1. The weight ratio of modifier preparation ingredients is as follows:

[0021] Polyethylene glycol 8

[0022] Anhydrous sodium sulfate 1

[0023] Cyanuric chloride 1.1

[0024] Anhydrous benzene 6.6

[0025] 2. Molecular modification (enzyme preparation) method of corn SOD:

[0026] A. Activation of modifiers:

[0027] Mix 24g of polyethylene glycol-6000 (molecular weight: 60000), 3.0g of anhydrous sodium sulfate and 3.3g of cyanuric chloride into 20g of anhydrous benzene, stir at room temperature for 12 hours, and filter. The product was precipitated with 332 g of ether, and then 50.3 g of benzene was used to dissolve the precipitate. Such precipitation and dissolution were repeated several times until no cyanuric chloride was absorbed by ultraviolet detection. Vacuum-dried to obtain a white solid powder, that is, activated polyethylene glycol-6000, which was sealed and stored in a refrigerator.

[0028] B. Activated polyethylene glycol-6000 modified SOD:

[0...

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Abstract

The invention relates to corn SOD molecule finishing method. Its features are that finishing agent and weight proportion are as follows: polyethylene glycol 8, anhydrous sodium sulfate 1, cyanogens urea acyl chloride 1, and anhydrous benzene 6.6; water and fat solubility polymers polyethylene glycol is used as the main finishing agent; the cyanogens urea acyl chloride is used as activator. The manufacturing method is simple. And it can increase stability, prolong half life, remove immunizing source and toxicity, increase film permeability, improve biochores and metabolism behavior, and increase solubility and organic solvent proof denaturing to make corn SOD can be extensively applied to food service, light, and medicine industries.

Description

technical field [0001] The invention belongs to an enzyme molecular modification method. Background technique [0002] Superoxide dismutase (SOD) can scavenge superoxide anion free radicals in the human body, protect cell membranes, DNA and other structures, so the research on SOD has received extensive attention. In the early 1950s, dermatology in the United States and other countries continued to develop, and SOD cosmetics were produced on this basis. At present, due to its better anti-radiation effect, it is very popular in the world. The sunscreen effect of SOD cosmetics is that Cu.Zn-SOD has a certain absorption effect on ultraviolet rays with a wavelength of 258-268nm. Mn-SOD and Fe-SOD have a strong absorption effect on ultraviolet rays with a wavelength of 280nm, thereby preventing the damage of ultraviolet rays to people. In foreign countries, SOD as a medicinal enzyme has been used in the Phase III treatment of traumatic brain injury, while domestic medicinal SO...

Claims

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Application Information

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IPC IPC(8): C12N9/08C12N9/96
Inventor 樊金娟陈红漫阚国仕阮燕晔崔振海
Owner SHENYANG AGRI UNIV
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