30 results about "Rhodobacter species" patented technology

The present invention relates to production of ubiquinone-10 in E. coli by isolating and sequencing a structural gene dds1 of decaprenyl diphosphate synthase derived from photosynthetic bacteria such as Rhodobacter capsulatus., and expressing it in E. coli.

The invention discloses a method of treating piggery methane fluid by utilizing composite photosynthetic bacteria, and relates to the direction of wastewater treatment and achieves the purpose of waste recycling. According to the method, the composite photosynthetic bacteria which contain budding bacteria and rhodobacter capsulatus are prepared; the piggery methane fluid is treated by utilizing the composite photosynthetic bacteria according to a dosage amount of (1 to 5) to (1 to 10) of the volume ratio of the composite photosynthetic bacteria to the methane fluid, and the processing temperature is 25 to 32 DEG C, and the illumination intensity is 2000 to 5000 lux. According to the method, methane fluid wastewater is treated by utilizing the synergistic effect of mixed bacteria, and compared with a method of treating the methane fluid by utilizing single bacterium, the removal efficiency of COD (chemical oxygen demand) is increased by more than 20%. According to the method, the synergistic effect of two types of photosynthetic bacteria is utilized, so that the growth rates of the two types of photosynthetic bacteria in the piggery methane fluid are increased, thereby increasing the utilization rate of the photosynthetic bacteria for organic matter, ammonia nitrogen, phosphorus and the like in the piggery methane fluid, overcoming the defect of poor treatment effect of the single bacterium on the methane fluid and increasing the treatment and purification efficiency of the composite bacteria for the methane fluid. Additionally, a large number of output bacteria can be comprehensively utilized directly, so that the purposes of removing pollutants and obtaining bacteria resources are achieved.

The invention discloses rhodobacter capsulatus and application thereof. The collection number of the rhodobacter capsulatus AYGHJ-16 is CCTCC NO: M 2011377; the rhodobacter capsulatus belongs to photosynthetic bacteria; and through the rhodobacter capsulatus, certain organic small molecules (such as organic acid and amino acid) can be converted into growth-stimulating substances and anti-virus substances by using light energy, so that growth of plants is stimulated, and the disease-resistant immunity of the plants is improved. Moreover, through the photosynthetic bacteria, product feedback inhibition of heterotrophic microbes can be released by using organic acid produced by metabolism of the heterotrophic microbes in soil, so that a large quantity of heterotrophic microbes are propagated, and conversion and absorption of certain inorganic substances in the soil are promoted; and the photosynthetic bacteria have strong nitrogen fixation and carbon fixation effects, so more inorganic substances can be provided for plants. The selenium-rich microbial agent can improve the micro-ecological environment of the soil, so that the disease resistance of the plants and internal absorption of various mineral elements and rare elements (such as selenium) are improved, and the selenium-rich requirements of the plants are met.

The invention discloses a method for efficiently preparing salvianolic acid components from overground stems and leaves of salviae miltiorrhizae. The method comprises the following steps: extracting overground stems and leaves of salviae miltiorrhizae, and carrying out bioconversion through photosynthetic bacteria including rhodopseudomonas palustris, green sulphur rhodop seudanonas palustris, rhodobacter sphaeroides, rhodobacter capsulatus and the like to prompte rise of salvianolic acid components in the stems and leaves, wherein the content of an active component salvianolic acid B is 3-4 times of that of the medicinal material, namely roots of the salviae miltiorrhizae, and the content of rosmarinic acid is dozens of times higher than that of the medicinal material salviae miltiorrhizae; and carrying out separation and purification through a macroporous resin column, so as to efficiently obtain the high-content extracts of phenolic acid components such as the salvianolic acid B, tanshinol, rosmarinic acid, ferulic acid and caffeic acid. The salvianolic acid components disclosed by the invention have good efficacies of resisting oxidation, protecting vascular endothelial cells, and promoting blood circulation to remove blood stasis, and can be applied to preparation of medicines or functional health products for preventing and treating cardiovascnlar and cerebrovascular diseases.

The invention discloses prawn feed containing rhodobacter capsulatus strain. The prawn feed containing rhodobacter capsulatus strain is characterized by being prepared from prawn feed, rhodobacter capsulatus strain fermentation liquor and sodium alginate, wherein 10g of odium alginate and 20mL of rhodobacter capsulatus strain fermentation liquor are added into each 100g of prawn feed until concentration is 3-6*107CFU (Colony Forming Unit) / g; the rhodobacter capsulatus strain is a (i) Rhodobacter capsulatus( / i) R3 strain and has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No:11753. The rhodobacter capsulatus is used as a feed additive to manufacture the prawn edible feed, a feeding result shows that the growth speed of the prawn which eats the feed in which the rhodobacter capsulatus is added is greatly improved compared with the prawn which eats common feed, and immunity is obviously improved. Since the rhodobacter capsulatus is used for manufacturing the feed, the production period is short, the production cost is low, a production condition can be easily controlled, the antimicrobial activity is high, the immunity can be obviously enhanced and the like, and the prawn feed has the potential of industrial production and has a good production application prospect.

The invention discloses a rhodobacter capsulatus strain DC-1 and application thereof. The collection number of the strain in China Center for Type Culture Collection is CCTCC NO: M2016676. The strain disclosed by the invention refers to photosynthetic bacteria capable of efficiently degrading benzene series and formaldehyde, can be used for treating sewage polluted by the benzene series and formaldehyde and is high in tolerance concentration of the benzene series and excellent in degradation effect; and moreover, the strain has capacity of removing the formaldehyde, the culture conditions of the strain are simple, and industrialized implementation is easily realized.

The invention discloses rhodobacter capsulatus and an application thereof. The invention provides rhodobacter capsulatus BN-W-6 which has a collection number of CGMCC No.8311 and belongs to photosynthetic bacteria. The rhodobacter capsulatus is fermented by adding an energy source after enlargement cultivation to obtain fermentation products including chlorophyll a, carotenoid, ketone-carotenoid, hydrogenase, catalase and the like. Biological enzymes generated by the fermentation products of the rhodobacter capsulatus in a pond can ensure that macroscopic algae are gradually decomposed, the products including chlorophyll a, carotenoid and the like can absorb illumination wavebands necessary for growth of the macroscopic algae to make the macroscopic algae shrunk and agglomerated until death, and the dead macroscopic algae can not influence bottom matters, thereby achieving the aims of controlling rampant growth of algae and not influencing the normal growth of sea cucumbers. The fermentation broth of the rhodobacter capsulatus provided by the invention can be used for inhibiting and removing the macroscopic algae, so that the culture density is increased, the culture environment is improved, and the aim of improving the pond culture output of aquatic organisms is achieved.

The invention discloses a screening method of rhodobacter capsulatus and an application thereof. The collection register number of the rhodobacter capsulatus is CGMCC No:11753. In the invention, the rhodobacter capsulatus is used as a feed additive for preparing a prawn feed; the feeding result indicates that the growth speed of the prawns eating the feed added with the rhodobacter capsulatus is remarkably increased in comparison with that the prawns eating common feed while the immunity is obviously improved; a method for preparing the feed by adopting the rhodobacter capsulatus has the advantages that production cycle is short, production cost is low, growth conditions are easy to control, antibacterial activity is high, immunity is obviously improved and the like; and moreover, the rhodobacter capsulatus totally has potential for industrial production and gets relatively good production and application prospect.

The invention discloses a method for cultivating a photosynthetic bacteria agent, comprising the following steps: A. Culture medium preparation: prepare pure water to be slightly alkaline, with a pH value of 7.5-8.5, and add 0.3% peptone, lemon Sodium acid 0.3%, yeast extract 0.2%, sodium bicarbonate 0.2%, sodium chloride 0.2%, ammonium chloride 0.2%, magnesium sulfate heptahydrate 0.2%, potassium dihydrogen phosphate 0.2%, and the balance is pure water; B. Selected strains: select effective live strains, and the strains include Rhodopseudomonas sphaericus and Rhodopseudomonas paluscens, mixed according to the mass percentage of 1:1.5; Among them, Rhodopseudomonas palustris is cultured by double-layer plate culture method Cultivate until the red single bacterium colony appears; C. Seed liquid culture: the mixed live strain that above-mentioned B step gained is fermented and cultivated, and the temperature of cultivation is 27-32 ℃, and illumination condition is 2500Lx~3000Lx; Adopt the present invention can effectively improve Cultivation efficiency of photosynthetic bacteria inoculum.

The invention discloses a prawn feed additive containing Rhodobacter Capsulatus and Bacillus subtilis strains. The prawn feed additive is composed of a prawn feed, a Rhodobacter Capsulatus strain fermentation liquid, a Bacillus subtilis strain fermentation liquid and sodium alginate, wherein 10g of sodium alginate is added to every 100g of the prawn feed, and culturing is carried out until the OD600 is 0.6, the total volume of the Rhodobacter Capsulatus strain fermentation liquid and the Bacillus subtilis strain fermentation liquid is 20mL and the final concentration is 3-6 * 10<7> / g. A result of feeding with prawn feeds prepared by using Rhodobacter Capsulatus and Bacillus subtilis as feed additives shows that the prawns fed with the feeds containing Rhodobacter Capsulatus and Bacillus subtilis have greatly higher growth speed and obviously high immunity than prawns fed with common feeds; and the feeds prepared by using Rhodobacter Capsulatus and Bacillus subtilis have the advantages of short production period, low production cost, easy control of growth conditions, high antibiotic activity, obviously enhanced immunity, high industrial production potential and good production application prospect.

The invention discloses a method capable of effectively treating high-ammonia nitrogen sewage, and aims at solving the problems of complicated process flow, serious secondary pollution, difficulty in maintaining strain advantages and the like caused by pretreatment in the traditional photosynthetic bacteria sewage treatment process at present and aims to provide a composite microbial inoculum for degrading the high-ammonia nitrogen sewage. The composite microbial inoculum for degrading the high-ammonia nitrogen sewage is obtained by mixing Rhodobacter capsulatus 3Z, Rhodobacter sphaeroides fermentation liquor, Rhodopseudomonas palustris fermentation liquor, Rhodobater johrii fermentation liquor and Bacillus subtilis fermentation liquor. The preparation method has simple process, can efficiently convert inorganic nitrogen into organic nitrogen, and obviously improves outstanding practicability of sewage treatment effect.

The invention discloses rhodobacter capsulatus and an application thereof. The invention provides rhodobacter capsulatus BN-W-6 which has a collection number of CGMCC No.8311 and belongs to photosynthetic bacteria. The rhodobacter capsulatus is fermented by adding an energy source after enlargement cultivation to obtain fermentation products including chlorophyll a, carotenoid, ketone-carotenoid, hydrogenase, catalase and the like. Biological enzymes generated by the fermentation products of the rhodobacter capsulatus in a pond can ensure that macroscopic algae are gradually decomposed, the products including chlorophyll a, carotenoid and the like can absorb illumination wavebands necessary for growth of the macroscopic algae to make the macroscopic algae shrunk and agglomerated until death, and the dead macroscopic algae can not influence bottom matters, thereby achieving the aims of controlling rampant growth of algae and not influencing the normal growth of sea cucumbers. The fermentation broth of the rhodobacter capsulatus provided by the invention can be used for inhibiting and removing the macroscopic algae, so that the culture density is increased, the culture environment is improved, and the aim of improving the pond culture output of aquatic organisms is achieved.

The invention relates to the technical field of wastewater treatment and bacterial fertilizer preparation, in particular to a method for preparing an ecological bacterial fertilizer from aerobic pig manure wastewater. The method comprises the following steps: culturing photosynthetic bacteria rhodopseudomonas palustris, rhodobacter capsulatus and rhodobacter sphaeroides by taking aerobic stage tank effluent of an anaerobic-anoxic-aerobic three-stage wastewater treatment system of pig manure wastewater as a raw material; the ecological bacterial fertilizer can be prepared from high-potassium-content aerobic pool pig manure wastewater effluent through one-time culture by compounding the same kind of bacteria, so that the degradation efficiency of organic matters, ammonia nitrogen and toxic and harmful substances in the wastewater is improved, and the method has the advantages of simple strain preparation and wastewater treatment processes, low cost and the like; the prepared ecological bacterial fertilizer is rich in various nutrient elements and photosynthetic bacteria, can significantly improve the metabolic activity of plant growth, photosynthesis and the like, quickly increases the contents of available phosphorus and available potassium in soil, effectively improves the yield and quality of plants, and improves phosphorus-deficient and potassium-deficient barren soil.

The invention provides an aquaculture tail water treatment system and treatment method. The treatment system comprises a settling zone, a filtering zone and a filter-feeding animal treatment zone. A composite flora consisting of rhodospirillum rubrum, rhodobacter capsulatus, rhodopseudomonas palustris and rhodotorula mucilaginosa is added into the filter-feeding animal treatment zone. According to the method, the culture tail water is treated by screening the microorganisms with the optimal proportion and combining the microorganisms with the filter-feeding animals. The composite microbial inoculant is combined with filter-feeding animals, and the pilot plant test proves that the effect is good. According to the invention, a composite microecological preparation based on photosynthetic bacteria is developed and combined with filter feeding shellfish to treat aquaculture tail water, so that a very good effect is achieved.

The invention discloses a strain of photosynthetic bacterium Rhodobacter capsulatus, a bactericide agent as well as a preparation method and an application of the bactericide agent. The strain of the photosynthetic bacterium Rhodobacter capsulatus is photosynthetic bacterium Rhodobacter capsulatus A12, and Rhodobacter capsulatus A12 is collected in CCTCC (China Center for Type Culture Collection) with the collection number of CCTCC No:M 2018604. The culture process for the photosynthetic bacterium Rhodobacter capsulatus A12 is simple, the culture time is short, the cost is low, and a biocontrol bactericide and biocontrol fermentation liquor for control of rice bacterial leaf blight are prepared from the strain of the photosynthetic bacterium Rhodobacter capsulatus with a simple and fast low-cost operating method.

The invention relates to an ecological method for treating excrement. The method comprises the step of performing solid-liquid separation on the excrement to obtain waste liquid and solid waste, wherein the waste liquid is used for planting pennisetum hydridum or pennisetum purpureum after being treated by the compound microorganism preparation, and the solid waste is used for culturing the earthworms or fly maggots; the planted pennisetum hydridum or pennisetum purpureum is used for livestock or fish culture; the compound microorganism preparation is mainly prepared from 70%-80% of rhodobacter capsulatus, 5%-20% of spherical bacteria and 5%-15% of pseudomonas gelatinasa. By use of the ecologic method disclosed by the invention, the pollution on the surrounding environment by the culture industry is relieved, and the quality security of the cultured aquatic product is guaranteed at the same time; the waste liquid is treated by adding the specific microorganism preparation, the existent odor is removed, the air environment of the mountain area is beautified, and the good ecologic benefit and social benefit are provided.

The invention provides a rhodobacter capsulatus 5-aminolevulinic acid synthetase mutant and application thereof. The amino acid sequence of the rhodobacter capsulatus 5-aminolevulinic acid synthetase mutant is as shown in SEQ ID NO. 1. Compared with wild type rhodobacter capsulatus 5-aminolevulinic acid synthetase, the rhodobacter capsulatus 5-aminolevulinic acid synthetase mutant has the advantages that the yield of 5-aminolevulinic acid in host cells is increased by about 22%; in the presence of 20 [mu] M heme, the mutant 5-aminolevulinic acid synthetase C201A can maintain relatively high relative enzyme activity.

The invention relates to: a method of treating liquid waste (liquid agricultural or industrial effluents or aquatic sites) which is loaded or polluted with tributyl phosphate (TBP), modified bacterial strains which can be used in the aforementioned treatment method, a method for monitoring changes in TBP pollution, and the device which is used to perform said treatment method. According to the invention, the liquid waste-treatment or -purification method essentially comprises: steps (1) consisting in bringing said liquid waste into contact with at least one non-sulphur purple photosynthetic bacterial strain which is resistant to TBP and which is selected from the group containing Rhodopseudomonas palustris (Rp. palustris), Rhodospirillum rubrum (Rs. rubrum), Rhodobacter capsulatus (Rb. capsulatus) or Rhodobacter sphaeroides (Rb. Sphaeroides) as well as the aforementioned modified bacterial strains in order to overexpress cytochrome P450 in conditions that enable the degradation of the TBP present in said waste, regardless of the initial TBP concentration; and (2) the recovery of the purified liquid effluents.

The invention relates to the technical field of wastewater treatment and plant planting, in particular to a method for preparing an ecological bacterial fertilizer from pig manure wastewater subjectedto anaerobic treatment. According to the method for preparing the ecological bacterial fertilizer by using the pig manure wastewater, provided by the invention, anaerobic tank effluent of an anaerobic-anoxic-aerobic three-stage wastewater treatment system of the pig manure wastewater is used as a raw material, and photosynthetic bacteria rhodopseudomonas palustris, rhodobacter capsulatus and rhodobacter sphaeroides are used for culturing and preparing the ecological bacterial fertilizer. The ecological bacterial fertilizer can be prepared from pig manure wastewater in an anaerobic tank through one-time culture by compounding use of the same kind of bacteria, the degradation efficiency of organic matters, ammonia nitrogen and toxic and harmful substances in the wastewater is remarkably improved, and the method has the advantages of simple strain preparation and wastewater treatment processes, low cost and the like; the prepared ecological bacterial fertilizer is rich in various nutrient elements and photosynthetic bacteria, can significantly improve the metabolic activity of plant growth, photosynthesis and the like, enhances the soil fertility, and effectively improves the yield and quality of plants.

The invention relates to a treatment agent for a textile waste water chamber. The treatment agent is prepared from the following materials in parts by weight: 70-100 parts of Coniogramme intermedia Hieron., 70-90 parts of broad-leaf pellionia radicans, 60-80 parts of Cardamine macrophylla Willd., 50-70 parts of Salvia substolonifera Stib., 60-70 parts of goldfussia, 50-80 parts of stemless launaea herb, 50-70 parts of common oxyspora root, 50-70 parts of Ziziphora bungeana and 30-80 parts of Celastrus gemmatus Loes, wherein the treatment agent is used for killing rhodobacter capsulatus ATCC 11166. The treatment agent for the texile waste water chamber is lower in cost, better in safety, obvious in disinfection effect and suitable for large-scale promotion.

The invention discloses a strain of capsulated rhodobacter ( Rhodobacter capsulatus strain) DC‑1, and its preservation number in the China Type Culture Collection Center is CCTCC NO: M2016676, the strain of the present invention is a photosynthetic bacterium that can efficiently degrade benzene series and formaldehyde, and can be used to treat sewage polluted by benzene series and formaldehyde , high tolerance concentration of benzene series, strong degradation effect; ability to remove formaldehyde, and the culture condition of this strain is simple, easy for industrial implementation.

The invention discloses a method for improving the oil yield of microalgae in a photoheterotrophic system. The method comprises the following steps: carrying out enlarged culture on microalgae in an autotrophic culture medium, centrifuging, and suspending in a photoheterotrophic culture medium to obtain a microalgae solution; performing amplification culture on rhodobacter capsulatus in a photosynthetic bacterium culture medium, centrifuging, and suspending in a photoheterotrophic culture medium to obtain rhodobacter capsulatus liquid; and inoculating the microalgae liquid and the rhodobacter capsulatus liquid into a photoheterotrophic culture medium, and carrying out photoheterotrophic culture. The microalgae and the rhodobacter capsulatus are subjected to symbiotic culture in the photoheterotrophic culture medium, so that the oil yield of photoheterotrophic culture microalgae is increased, the biomass yield of the culture system can be increased by 65% or above compared with that of a pure microalgae system, and the fatty acid yield can be increased by 30% compared with that of the pure microalgae system.

The present invention provides a rhodobacter capsulated 5-aminolevulinic acid synthase mutant and its application. The amino acid sequence of the capsulated rhodobacter 5-aminolevulinic acid synthase mutant is shown in SEQ ID NO.1. Compared with the wild-type Rhodobacter capsula 5-aminolevulinic acid synthase mutant of the present invention, the production of 5-aminolevulinic acid in host cells is increased by about 22%; in the presence of 20 μM heme, the mutant 5‑aminolevulinic acid synthase C201A can maintain a relatively high relative enzyme activity.

The invention relates to specific PCR primers for identifying rhodobacter capsulatus, a kit and application. The specific PCR primers comprise the following primer pairs: primer pairs shown in SEQ ID NO:1 and SEQ ID NO:2; primer pairs shown in SEQ ID NO:3 and SEQ ID NO:4; and primer pairs shown in SEQ ID NO:5 and SEQ ID NO:6. The specific PCR primers provided by the invention have high specificity to the rhodobacter capsulatus and solve the problem of poor capsule specificity in traditional methods. A method for identifying the rhodobacter capsulatus established by the primers has the advantages of good specificity, high stability and high sensitivity, can well control occurrence of false positive during detection and improves the detection accuracy of the rhodobacter capsulatus.

The invention discloses a photosynthetic bacteria strain, a biocontrol agent, biocontrol fermentation liquid, a preparation method and an application thereof. The photosynthetic bacterial strain is rhodobacter capsulatus A18. (1) The photosynthetic bacteria rhodobacter capsulatus A18 of the invention has simple production process, short cultivation time and low cost, and the strain can be preparedby a simple, rapid and low-cost operation method to obtain the biocontrol agent and the biocontrol fermentation broth for preventing and controlling citrus canker disease. (2) The biocontrol agent and the biocontrol fermentation broth prepared by the photosynthetic bacteria rhodobacter capsulatus A18 of the invention are environmentally friendly, and non-toxic to humans and animals, have no phytotoxicity to crops, are simple and convenient to apply, and are not easy to cause drug resistance for insect disease.

The invention relates to a rhodobacter capsulatus strain RC1. The rhodobacter capsulatus strain RC1 is preserved in the China Center for Type Culture Collection, and is assigned with the accession number of CCTCC: M 2017323.The rhodobacter capsulatus is photosynthetic bacterium obtained by adopting microbes in sludge water on the bottom layer of a constructed wetland anaerobic pool domesticated byformaldehyde as a material, and carrying out separation by adopting inorganic salt culture medium of the photosynthetic bacterium, the rhodobacter capsulatus is gram negative bacterium, the bacteriumsolution is blood red, and carotenoid and bacteriochlorin a exist in bacterium cells. The rhodobacter capsulatus can grow in liquid inorganic salt culture medium containing 4 or 6 mM formaldehyde under illumination, the removal rate for formaldehyde added in the culture medium achieves 100%, formaldehyde is converted into glucose through metabolism via the photosynthetic carbon assimilation path,and the photosynthetic carbon assimilation path is the main mechanism for detoxification of formaldehyde.

The invention discloses rhodobacter capsulatus and application thereof. The collection number of the rhodobacter capsulatus AYGHJ-16 is CCTCC NO: M 2011377; the rhodobacter capsulatus belongs to photosynthetic bacteria; and through the rhodobacter capsulatus, certain organic small molecules (such as organic acid and amino acid) can be converted into growth-stimulating substances and anti-virus substances by using light energy, so that growth of plants is stimulated, and the disease-resistant immunity of the plants is improved. Moreover, through the photosynthetic bacteria, product feedback inhibition of heterotrophic microbes can be released by using organic acid produced by metabolism of the heterotrophic microbes in soil, so that a large quantity of heterotrophic microbes are propagated, and conversion and absorption of certain inorganic substances in the soil are promoted; and the photosynthetic bacteria have strong nitrogen fixation and carbon fixation effects, so more inorganic substances can be provided for plants. The selenium-rich microbial agent can improve the micro-ecological environment of the soil, so that the disease resistance of the plants and internal absorption of various mineral elements and rare elements (such as selenium) are improved, and the selenium-rich requirements of the plants are met.