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Method for improving oil yield of microalgae in photoheterotrophic system

A technology of oil production rate and microalgae, which is applied in the field of improving oil production rate of microalgae under photoheterotrophic conditions, can solve the problems of low oil production rate of microalgae and the like, and achieve the effect of improving oil production rate

Active Publication Date: 2021-10-19
ANHUI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, when microalgae were cultured alone under photoheterotrophic conditions, the oil production rate of microalgae was low

Method used

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  • Method for improving oil yield of microalgae in photoheterotrophic system
  • Method for improving oil yield of microalgae in photoheterotrophic system
  • Method for improving oil yield of microalgae in photoheterotrophic system

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Effect test

Embodiment 1

[0053] A method for improving the oil production rate of microalgae in photoheterotrophic system, comprising the following steps:

[0054] (1) Add the modified C medium into a 1L photosynthetic reactor, fill each bottle with 0.6L, and then sterilize it by high-pressure steam at 121°C for 20 minutes. Algae start autotrophic expansion culture, CO 2 The mixed gas with air enters the reactor after being sterilized by a 0.22μm filter at the top of the reactor, and the aeration rate is 0.5v / v / min, in which CO 2 The concentration is 4%, the temperature is controlled at 24±2°C, and the light intensity is 8000lux; the culture time is 7 days, and the microalgae are collected by centrifugation at 6000 rpm for 10 minutes after the culture is over, and the collected microalgae are suspended in the photoheterotrophic medium The microalgae liquid is obtained in the microalgae liquid, and the biomass in the microalgae liquid is 10-15g / L.

[0055] (2) Add 112Van Niel's yeast agar medium into...

Embodiment 2

[0062] A method for improving the oil production rate of microalgae in photoheterotrophic system, comprising the following steps:

[0063] (1) Add the modified C medium into a 1L photosynthetic reactor, fill each bottle with 0.6L, and then sterilize by high-pressure steam at 121°C for 20 minutes. The algae started autotrophic expansion, CO 2 The mixed gas with air enters the reactor after being sterilized by a 0.22μm filter at the top of the reactor, and the aeration rate is 0.5v / v / min, in which CO 2 The concentration is 4%, the temperature is controlled at 24±2°C, and the light intensity is 8000lux; the culture time is 7 days, after the culture is completed, centrifuge at 6000 rpm for 10 minutes to collect Chlorella pyrenoidosa, and suspend the collected Chlorella pyrenoidosa The microalgae liquid is obtained in the photoheterotrophic culture medium, and the biomass in the microalgae liquid is 10-15g / L.

[0064] (2) Add 112Van Niel's yeast agar medium into a 250mL screw-nec...

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Abstract

The invention discloses a method for improving the oil yield of microalgae in a photoheterotrophic system. The method comprises the following steps: carrying out enlarged culture on microalgae in an autotrophic culture medium, centrifuging, and suspending in a photoheterotrophic culture medium to obtain a microalgae solution; performing amplification culture on rhodobacter capsulatus in a photosynthetic bacterium culture medium, centrifuging, and suspending in a photoheterotrophic culture medium to obtain rhodobacter capsulatus liquid; and inoculating the microalgae liquid and the rhodobacter capsulatus liquid into a photoheterotrophic culture medium, and carrying out photoheterotrophic culture. The microalgae and the rhodobacter capsulatus are subjected to symbiotic culture in the photoheterotrophic culture medium, so that the oil yield of photoheterotrophic culture microalgae is increased, the biomass yield of the culture system can be increased by 65% or above compared with that of a pure microalgae system, and the fatty acid yield can be increased by 30% compared with that of the pure microalgae system.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, and relates to a method for improving the oil production rate of microalgae under photoheterotrophic conditions. Background technique [0002] Biodiesel, namely fatty acid methyl ester, is a kind of biomass energy with renewable characteristics. It is mainly derived from oil-producing crops, such as soybeans and sunflower seeds, but its further development is limited by shortcomings such as low yield, high cost, and competition for land with other crops. A portion of microalgae is rich in oil and has become a new source of biodiesel due to its short growth cycle and no need to occupy arable land. In the commercial process of microalgae oil production, the high production cost is the primary bottleneck problem faced by microalgae to produce biodiesel. How to reduce the commercialization cost of algae oil production has become a topic of great research value, and improving the oil producti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12N1/12C12N1/20C12R1/89C12R1/01
CPCC12P7/64C12P7/6409C12N1/12C12N1/20Y02E50/10
Inventor 申晓菲许亚萍张帅黄佳乐黄琦
Owner ANHUI NORMAL UNIV
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