The application relates to the field of
biotechnology, in particular to a nanobody targeting
annexin A1 and a preparation method and application thereof, discloses a nanobody targeting
annexin A1, the nanobody comprises a nanobody Nb6, wherein the
amino acid sequence of the nanobody Nb6 is shown in Seq.ID NO.1, the nanobody Nb4 provided has higher affinity with
annexin A1, the nanobody can be used for targeted action on annexin A1, has high-strength combination capacity with annexin A1, is favorable for development of a nanobody related
medicine of annexin A1, realizes targeted treatment and imaging of tumors, and has very great clinical application value. Meanwhile, a
molecular probe can be prepared based on the nanobody, the
molecular probe is specifically combined with a tumor
blood vessel surface annexin
antigen, is applied to specific imaging and diagnosis of tumors, and is used for delivery of related medicines, and is widely applied.