290 results about "Mucoprotein" patented technology

The invention discloses a mussel mucoprotein gel for repairing and reliving itching. The mussel mucoprotein gel is prepared from the following components in percentage by weight: 0.15%-0.2% of mussel mucoprotein, 0.5%-2% of a macromolecule host material, 3%-10% of a humectant, 1%-5% of a surfactant, 0.1%-0.5% of a preservative, 1%-2% of a protein protectant, 0.5%-3% of a stabilizer, 0.5%-1% of a bacteriostatic agent, 0.8%-5.2% of a pH modifier and the balance of purified water. The mussel mucoprotein gel has good biological adhesion, is rapidly and effectively adhered to a wound surface, is capable of rapidly reliving itching and easing pain, and has the advantages of being low in immunogenicity, good in biocompatibility with a human body, convenient to use, free of thrill to skin, safe, nontoxic and free of bad reaction.

The invention provides a mussel mucoprotein gel for wound repair, and a preparation method and an application thereof. The mussel mucoprotein gel for wound repair comprises the following raw materials in percentage by weight: 0.15-0.2% of mussel mucoprotein, 0.1-5% of protein protector, 0.1-5% of tackifier, 0.1-5% of stabilizer, 0.1-5% of preservative, 1-10% of wetting agent, 0.1-5% of pH (potential of hydrogen), and 64.8-98.35% of medical dissolving water. With Carbomer as a substrate, the mussel mucoprotein gel has good extending property, is easy to coat and adhere on a skin, has no irritation to skin and mucosa and can absorb tissue penetrating fluid, so secretions can be exhausted conveniently; the gel is not greasy, the medicines are released fast, and the coupling effect with skin tissues is excellent.

The invention discloses a method for effectively culturing tumor infiltrating lymphocytes (TILs). The method comprises the following steps: extracting mononuclear cells from the pectoral ascite or tumor tissue of a patient, washing, inoculating into a culture bottle coated with recombinant human fibrin and cluster-of-differentiation-3 antibody, culturing for 24 hours, adding interleukin II, then using a serum-free culture medium containing the interleukin II and the supernatant pectoral ascite to enlarge and subculture every two or three days, and adding apoptosis-regulated protein survivin and mucoprotein-1 on the twelveth or thirteenth day to activate the killing activity; and harvesting cells on the fourteenth day. By adopting the method, the culture time, which is only 14 days, is greatly shortened, so that the hospital stays of the patient can be greatly shortened and the hospital burden of the patient can be reduced. Meanwhile, the problems that the cell proliferation speed is low and the proliferation ratio is low can be solved, thus the number of the cultured cells can meet the clinical requirement. The cultured TILs has strong cell specificity so as to enhance the clinical curative effect.

The invention discloses a preparation method of a concrete artificial fish reef material, and belongs to the technical field of artificial fish reef preparation. The preparation method comprises the steps that firstly, pretreated coal slag and clay are mixed and calcined; then, obtained materials are mixed with gypsum and the like; then, the mixture is added into a mold and is subjected to primary maintenance in solution cavity environment; carbon dioxide in a solution cavity takes a reaction with sodium hydroxide generated in the materials to generate calcium carbonate, so that the alkalinity is reduced; calcium hydroxide and ettringite are reduced; the materials are demolded to obtain fish reef concrete; then, a mixed solution prepared by chitosan and the like is sprayed and coated on the surface of the concrete; maintenance is performed again to obtain the concrete artificial fish reef material. The preparation method solves the problems of low artificial fish reef intensity, poor durability, short service life and the like. The obtained concrete artificial fish reef material has high intensity and higher durability; the hardened concrete artificial fish reef material has good compactness; the finally coated chitosan and mussel mucoprotein can be used as microorganism growth nutrient substances; the microorganism attachment quantity is increased.

The invention discloses a method for preparing a high-purity taste-removing purple sweet potato pigment, and belongs to the field of food additives and agricultural product deep processing. The method for preparing the high-purity taste-removing purple sweet potato pigment comprises the steps that 1, pretreatment is conducted on purple sweet potato raw materials; 2, the purple sweet potato pigment is extracted from low-frequency microwave auxiliary acidified aqueous solution; 3, preliminary purification is conducted on macroporous resins; 4, high-speed counter-current chromatography separation and purification are conducted, and the taste-removing purple sweet potato pigment with concentration more than 95% is obtained. According to the method for preparing the high-purity taste-removing purple sweet potato pigment, the microwave auxiliary acidified aqueous solution is used for extraction, flocculants are added to flocculate mucoproteins, then macroporous resin static adsorption and dynamic elution are conducted, and the taste-removing and enrichment purification process is preliminarily accomplished, and the high-purity taste-removing purple sweet potato pigment is finally prepared through the high speed counter-current chromatography method in a separation mode. According to the method for preparing the high-purity taste-removing purple sweet potato pigment, technological processes are advanced, separation efficiency is high, recovery rate is high, product purity is more than 95%, preparation amount is large, an obtained product is free of peculiar smell, has few impurities and is good in solubility, and method for preparing the high-purity taste-removing purple sweet potato pigment is easy to popularize and use.

The invention provides a process for extracting yam mucoproteins from yams and belongs to the field of extracting functional biological macromolecular substances from natural materials to produce functional healthcare food. The extracting process comprises the main steps of (1) material selection; (2) leaching gelatinization; (3) enzymolysis; (4) standing; (5) adjustment of PH value; (6) flocculation; (7) precipitation; and (8) drying. The yield of the yam mucoprotein products prepared by the process is 4 to 5 percent, and the content of yam mucoprotein is 60 to 67 percent. According to the process provided by the invention, the organic solvent extraction in the prior art is replaced by the flocculation and natural precipitation, so the product cost is greatly saved, the environmental pollution is lowered, and the process has great significance for actual production.

The invention discloses a composite wound restoration dressing based on mussel mucoprotein and polylactic acid microspheres, and a preparation method and an application thereof. The composite wound restoration dressing based on mussel mucoprotein and polylactic acid microspheres is obtained through mixing the mussel mucoprotein with the polylactic acid microspheres. The obtained novel restoration dressing has extremely strong adhesion, can be widely adhered to the surfaces of wounds, has very good biocompatibility and degradability, has no toxic or harmful effects on human bodies, and can accelerate the healing of the wounds.

The invention discloses a preparation method of saliva extracellular vesicles and application of the saliva extracellular vesicles to molecular diagnosis.The preparation method includes the steps of removing high-abundance proteins from saliva samples, filtering out mucoproteins, separating and extracting the extracellular vesicles, characterizing the obtained extracellular vesicles, extracting extracellular vesicle proteins, identifying the extracellular vesicle proteins and applying the extracellular vesicle proteins to lung cancer biomarker discovery.Currently, a normalized saliva extracellular vesicle preparation technology is absent, and high-abundance protein interference and background impurity interference, existing in existing preparation technologies, result in shortcomings of low extracellular vesicle recovery rate, fewer identified proteins and the like, so that true extracellular vesicle proteomics information can be reflected difficultly.The preparation method of the saliva extracellular vesicles and application of the saliva extracellular vesicles to molecular diagnosis have the advantages that saliva of healthy adult volunteers and lung cancer patients is taken as the samples, and an affinity chromatography and membrane separation combined technology is adopted, so that after the high-abundance proteins and the mucoproteins of the saliva are removed, the extracellular vesicles in the saliva are extracted centrifugally, proteomes of the extracellular vesicles are analyzed and more extracellular vesicle proteins are identified.

The invention discloses a bovine colostrum infant formula. The bovine colostrum infant formula comprises the following components in percentage by weight: 20 to 25 percent of whey protein concentrate, 0.2 to 1 percent of lactoferritin, 9 percent of galactooligosaccharides, 1 percent of fructooligosaccharides, 1 to 5 percent of mucoprotein and the balance of pure bovine colostrum powder. In the bovine colostrum infant formula, the bovine colostrum is combined with prebiotics; the immunity of an infant can be improved because the bovine colostrum is rich in immunoglobulin; the absorption of nutritive materials including the immunoglobulin is promoted by the perfect combination of FOS and GOS; and thus the immunity can be further improved. The perfect combination of FOS and GOS used in the formula can bring the prebiotics into play to the greatest extent.

The invention discloses an application of lactobacillus rhamnosus to preventing and relieving ulcerative colitis, and belongs to the technical field of functional microorganisms. The lactobacillus rhamnosus can tolerate the gastrointestinal environment of a human body, significantly reduce weight loss during the period of ulcerative colitis, improve fecal traits and hematochezia, improve colon mucosal injury, reduce MPO activity, and reduce the content of proinflammatory factors TNF-alpha, IL-1beta, IL-6 and IFN-gamma in colon. The transcription levels of colon close junction related proteins Claudin-3, ZO-1, ZO-2 and Occludin, antibacterial peptides Reg3g and Reg3b and mucoprotein MUC2 are up-regulated, the abundance of short-chain fatty acid producing bacteria Coprococcus and Faecalibacterium and the content of short-chain fatty acid are improved, the abundance of beneficial bacteria Lactobacillus in intestinal tracts is increased, the abundance of the conditional pathogenic bacteria Acinetobacter is reduced, and the abundance and diversity of intestinal flora are increased.

The invention relates to the technical field of agriculture microorganism genetic engineering, in particular to separation and cloning of a Helicoverpa armigera cadherin gene fragment hacad1and encoded polypeptide fragment HaCad1. By separation, the cadherin gene fragment hacad1 of 735bp is obtained from the midintestine of the Helicoverpa armigera, an encoded product thereof is the polypeptide fragment HaCad1 which can be combined with Bt toxin CrylAc10. Biological experiments indicate the polypeptide prepared in the invention has very strong enhancing insecticidal activity on insecticidal crystal protein CrylAc10 of lepidoptera insects. The invention also includes preparation and an application for recombinant Escherichia coli EMB1104 expressing the Helicoverpa armigera cadherin fragment HaCad1. The recombinant Escherichia coli EMB1104 is preserved in CCTCC, the preservation number is CCTCC NO: M209010.

The invention relates to a chimeric antigen receptor and an expression gene thereof, a double-antigen regulated type T cell modified by the chimeric antigen receptor, and application thereof. The expression gene of the chimeric antigen receptor comprises a first fusion protein expression gene and a second fusion protein expression gene, wherein the first fusion protein expression gene comprises a mucoprotein-1 antibody expression gene, a notch receptor expression gene and a Gal4-VP64 transcription activating protein expression gene which are sequentially connected; the second fusion protein expression gene comprises a Gal4-UAS promoter expression gene and an anti-mesothelin expression gene which are sequentially connected. By adopting the innovative design, the chimeric antigen receptor has the advantages that the chimeric antigen receptor can be successfully imported into the T cell to form the T cell modified by the chimeric antigen receptor; the immune reaction cannot be produced until the mucoprotein-1 and the anti-mesothelin signals simultaneously exist, so as to reach the controllable immune reaction of the chimeric antigen receptor, fewer side effects in treatment, and high specificity.

The invention relates to the technical field of mucin 1 detection, in particular to a fluorescent biological probe for mucin 1 detection, a sensor, application and a detection method. In the present invention, the aptamer probe is used for an Exo I assisted target circulation (EATR) reaction. The hairpin probe comprises a hairpin probe H1 and a hairpin probe H2 which are used for GO-assisted hybridization chain reaction (HCR); the aptamer probe and the hairpin probe are combined to be used for mucin 1 detection, so organic combination of Exo I-assisted target circulation reaction and GO-assisted hybridization chain reaction can be realized; therefore, cascade amplification of fluorescence signals is realized, the detection sensitivity of mucoprotein 1 is remarkably improved, the detectionlimit is reduced, quantitative detection of low-concentration mucin 1 can be realized, and theprobe has high specificity and can be used for specifically identifying and detecting mucin 1.

The invention provides a preparation method of a tumor dendritic cell vaccine sensitized by glycosylated antigen MUC1 (mucoprotein 1). Glycosylation modified tumor antigen peptide is used for sensitizing externally cultured DC (Dendritic Cell) and then the activated DC cells are transfused to a tumor patient. The invention further provides an application of the tumor DC cell vaccine sensitized by the glycosylated MUC1 antigen in treatment of breast cancer and pancreatic cancer. The preparation method provided by the invention has the following advantages (1) the DC cell vaccine amplification efficiency is high; (2) the DC cells have high antigen activity; (3) the DC cells have high maturity degree, and side effects such as immune tolerance are prevented; and (4) small side effects are caused.

The invention discloses hypoglycemic germinated brown rice juice and a preparation method thereof. The hypoglycemic germinated brown rice juice is prepared from germinated brown rice, blueberries, strawberries, chick-peas, pinto beans, onions, fresh bitter gourds, fresh folium mori, lactosucrose, yam mucoprotein, selenium-rich yeast powder, hydroxy-malonic acid, white granulated sugar, enzyme mixture, Angel saccharomyces cerevisiae, berberine and lysozyme. The hypoglycemic germinated brown rice juice and the preparation method have the advantages that the method is simple; the hypoglycemic germinated brown rice juice is exquisite in taste, delectable in sourness and sweetness, and rich in fruity flavor; as the enzyme mixture is added for enzymolysis, the hypoglycemic germinated brown rice juice is fine and smooth in taste, and the raw materials are converted into micromolecular nutritional ingredients which are easy to digest and absorb; various blood sugar regulating food materials and nutritional ingredients are added, so as to adjust blood sugar bidirectionally, assist therapy of diabetes mellitus, supplement comprehensive nutritional ingredients, enhance resistibility, build bodies, and delay aging; berberine and lysozyme can perform an excellent bactericidal effect, contain no additive, and are natural, wide, safe and healthy in source.

The invention discloses lysis solution of saliva nucleic acid extracted by a paramagnetic particle method. The lysis solution is characterized by comprising 10-150mM of Tris-HCl, 1-10M of chaotropic salt, 0.1-10% of negative ion surface active agents, 1-20% of nonionic surface active agents, 0.1-4% of CTAB (cetyl trimethyl ammonium bromide) and 1-100mM of chelating agents. The lysis solution extracted by the paramagnetic particle method sufficiently and effectively splits cells, can effectively remove special impurities such as mucopolysaccharide, mucoprotein and food residues and other conventional impurities such as protein, fat, carbohydrate and salt in saliva, so that the extraction effect of the nucleic acid is better, the purity of the nucleic acid is higher, more efficient late detection is ensured, and detection results are more accurate. The lysis solution is applicable to extraction of the nucleic acid of biological samples such as whole blood, cell cultures, bacterial cultures, swabs and tissues.

The invention discloses a method for detecting miRNA (micro ribonucleic acid) in stomach juice, which comprises stomach juice extraction and neutralization, RNA release, chloroform extraction, isopropanol precipitation, ethanol washing, RNA reverse transcription, PCR (Polymerase Chain Reaction) detection and other steps. In the detection method, a sodium hydroxide solution is used to liquefy viscous components in the stomach juice, so that mucoproteins and polysaccharides are suspended in the solution, and the stomach juice is neutralized; trypsinase is added to carry out digestion, so that the mucoproteins and other proteins can be hydrolyzed, and cell components in the stomach juice are loosened at the same time, thereby facilitating centrifugal separation; and a Trizol solution is added to carry out repeated blow and beat, so that the RNA is completely released and separated from the polysaccharides, the mucoproteins and other impurities, thereby solving the problem that the RNA extraction can be disturbed by the acidity of the stomach juice as well as polysaccharides, mucoproteins and the like in the stomach juice. Thus, the invention can eliminate the disturbance caused by polysaccharides, proteins and other impurities in the stomach juice, complete the efficient extraction of miRNA in the stomach juice and carry out fluorescent quantitative PCR detection on the extracted miRNA.

Provided is a test paper for testing chorionic gonadotropin in human saliva. The test paper comprises a primary sample pad, nitrocellulose filter membrane with testing T line and quality control C line, a sample suction pad and a bottom plate. The primary sample pad, the nitrocellulose filter membrane and the sample suction pad are respectively connected with the bottom plate through lap joints to form a test paper; among which the upper side of the primary sample pad is pasted with a secondary sample pad for filtering the impurities in the sample. The test paper has the advantages of 1, the design of the secondary sample pad in both single and double channel test papers can effectively filter the impurities in the saliva, especially the mucoprotein, miscellaneous bacteria and the like; 2, the sample in the double channel test paper passes through a first level channel to touch the test line without creating any composite substance through the combination with labeled antibody in the binding pad, the labeled antibody passes through a second level channel and touches the test line. This test paper overcomes the concentration/coagulation problem between the labeled sample and sample analyte.

The invention belongs to the field of medicinal preparations, and relates to liposome for eyes and a preparation method thereof. In order to overcome the defects that common eye drops have short residence time in the conjunctival sac to cause low bioavailability at the eyes and the semi-solid dosage form has poor compliance and is not easy to be accepted by patients and the like in the prior art,the invention provides a biological adhesive liposome preparation for eyes. The biological adhesive liposome preparation for the eyes consists of the liposome, a liposome membrane modification material and a medicament wrapped in the liposome, wherein the surface of the liposome is modified with free mercapto, and a covalent binding disulfide bond can be formed by the free mercapto and a mucoprotein subdomain rich in cysteine on the surface of the eye to anchor the liposome on the surface of a mucous membrane and serve as a medicament store to slowly release the medicament in the conjunctivalsac and provide permanent driving force for the absorption of the medicament. The preparation is helpful for promoting the absorption of the medicament at the eyes, and can improve the bioavailabilityof the medicament.

The invention relates to a composite dressing of a pig accellular dermal matrix and a preparation method of the composite dressing. The preparation method comprises the following steps: (1) washing: washing a skin piece in a phosphoric acid buffer salt solution; (2) separating epidermis and dermis: soaking and digesting the skin piece in a Dispase II solution to be decomposed into IV type collagen and fiber mucoprotein, separating epidermis and dermis, and then washing in the phosphoric acid buffer salt solution; (3) removing dermal cells, namely, soaking the skin piece in a Triton X-100 solution to further remove dermal cell components, and then washing in the phosphoric acid buffer salt solution; (4) carrying out ultrasonic washing, and then soaking in the phosphoric acid buffer salt solution for temporary storage; and (5) coating collagen: soaking the skin piece in I type collagen for filling and sealing, or soaking the skin piece in the I type collagen for freeze drying, sealing and packaging freeze dried substances to obtain finished products. According to the invention, cells are thoroughly removed, and the composite dressing is complete in fiber support, strong in applicability and good in application effect.

The invention provides bifidobacterium longum subsp. Longum KLDS K5 capable of preventing and relieving symptoms of colitis, and belongs to the technical field of microorganisms. The invention further provides a preparation method of the bifidobacterium longum subsp. Longum KLDS K5. Experiments show that the bifidobacterium longum provided by the invention can tolerate the human gastrointestinal environment, relieve weight loss in the ulcerative colitis disease period, improve colonic mucosa injury and reduce MPO activity, and inhibit oxidation-related factors through iNOS and COX-2 signal pathways so as to relieve inflammatory bowel diseases; nF-kappa B p65 nuclear transfer is inhibited through a TLR4/MyD88/NF-kappa B signal channel, the expression quantity of proinflammatory factors TNF-alpha, IL-1beta, IL-6 and IL-10 in colon is reduced, the transcriptional level of colon tight connection related proteins Claudin-1, ZO-1 and Occludin and mucoprotein MUC2 is up-regulated, the intestinal flora change after DSS induction can be improved in the genus level, and the richness and diversity of the intestinal flora are improved.

The invention provides a microbial composition with a weight losing effect. The microbial composition is prepared from lactobacillus gasseri and ackermansiella muciniphila, wherein the lactobacillus gasseri and the ackermansiella muciniphila are added into the microbial composition; the ratio of the viable count of the lactobacillus gasseri to the viable count of the mucophilic protein-ackermania is (2: 3)-(3: 2). By means of the probiotic composition containing lactobacillus gasseri and mucoprotein-ackermania in a specific ratio and specific content, the condition of abnormal body weight caused by metabolic diseases can be improved.

An artificial medical snake gall for treating cough, asthma, inflammation, etc and resolving phlegm contains at least 3 composite bile acids (taurocholic acid, tauro-chenodeoxycholic acid and taurodeoxycholic acid), as well as mucoprotein, cholesterol, lecithin, free bile acid, pile pigment, amino acid, trace elements, etc.