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Method for making multispecific antibodies having heteromultimeric and common components

The invention relates to a method of preparing heteromultimeric polypeptides such as bispecific antibodies, bispecific immunoadhesins and antibody-immunoadhesin chimeras. The invention also relates to the heteromultimers prepared using the method. Generally, the method provides a multispecific antibody having a common light chain associated with each heteromeric polypeptide having an antibody binding domain. Additionally the method futher involves introducing into the multispecific antibody a specific and complementary interaction at the interface of a first polypeptide and the interface of a second polypeptide, so as to promote heteromultimer formation and hinder homomultimer formation; and / or a free thiol-containing residue at the interface of a first polypeptide and a corresponding free thiol-containing residue in the interface of a second polypeptide, such that a non-naturally occurring disulfide bond is formed between the first and second polypeptide. The method allows for the enhanced formation of the desired heteromultimer relative to undesired heteromultimers and homomultimers.
Owner:GENENTECH INC

Nuclear reprogramming factor and induced pluripotent stem cells

The present invention relates to a nuclear reprogramming factor having an action of reprogramming a differentiated somatic cell to derive an induced pluripotent stem (iPS) cell. The present invention also relates to the aforementioned iPS cells, methods of generating and maintaining iPS cells, and methods of using iPS cells, including screening and testing methods as well as methods of stem cell therapy. The present invention also relates to somatic cells derived by inducing differentiation of the aforementioned iPS cells.
Owner:KYOTO UNIV

Anthranilamide compound as well as preparation method and application thereof

The invention discloses an anthranilamide compound as well as a preparation method and an application thereof. The compound has the chemical name as follows: 3-bromine-1-(3-Cl-2-pyridyl)-N-[4-methanethioamido-2-methyl-6-(methylaminoformyl)-phenyl-1H-pyrazol-5-methanamide, and is obtained through enabling bromine cyanide insect amide and a vulcanizing agent to react in the existence of a polar organic solvent and a catalyst at the temperature of 0-100 DEG C. The compound disclosed by the invention can be used for independently preparing an insecticide or preparing a mixed insecticide together with abamectin insecticides, neonicotinoid insecticides or pyrethroid insecticides, and the independently prepared insecticide is used for preventing and controlling Lepidoptera pests, dipteral pests or hemiptera pests.
Owner:江苏省农用激素工程技术研究中心有限公司 +1

Apparatus for supplying surgical staple line reinforcement

InactiveUS20090095791A1Thinner materialAccurately and successfully positionedSuture equipmentsStapling toolsBiomedical engineeringMaterial supply
An apparatus for supplying surgical buttress material to a surgical stapler is provided. The apparatus has a pivotable area for attaching surgical buttress material. The apparatus may also have an adhesive and a release liner disposed over the buttress material.
Owner:WL GORE & ASSOC INC

Antifungal polypeptide and methods for controlling plant pathogenic fungi

Antifungal polypeptides, isolated from Medicago plants, are shown to control fungal damage to plants. DNA encoding the polypeptides was cloned into vectors for transformation of plant-colonizing microorganisms or plants, thereby providing a method of inhibiting fungal growth on plants. The polypeptides can be formulated into compositions useful in controlling undesired fungi.
Owner:MONSANTO TECH LLC

Fluorinated surfactants for use in making a fluoropolymer

InactiveUS20070117914A1Convenient and easy preparationPrepared cost-effectivelyOrganic active ingredientsOrganic compound preparationEmulsion polymerizationCarboxylic acid
The present invention provides a fluorinated surfactant having the general formula:[Rf—(O)t—CQH—CF2—O]n—R-G  (I)wherein Rf represents a partially or fully fluorinated aliphatic group optionally interrupted with one or more oxygen atoms, Q is CF3 or F, R is an aliphatic or aromatic hydrocarbon group, G represents a carboxylic or sulphonic acid or salt thereof, t is 0 or 1 and n is 1, 2 or 3. The surfactant is particularly useful in polymerizing fluorinated monomers in an aqueous emulsion polymerization.
Owner:3M INNOVATIVE PROPERTIES CO

Nuclear reprogramming factor and induced pluripotent stem cells

The present invention relates to a nuclear reprogramming factor having an action of reprogramming a differentiated somatic cell to derive an induced pluripotent stem (iPS) cell. The present invention also relates to the aforementioned iPS cells, methods of generating and maintaining iPS cells, and methods of using iPS cells, including screening and testing methods as well as methods of stem cell therapy. The present invention also relates to somatic cells derived by inducing differentiation of the aforementioned iPS cells.
Owner:KYOTO UNIV

Method for transforming monocotyledons

The invention relates to a method for transforming a monocotyledonous plant. The time required from transformation to regeneration of a plant is shorter using the inventive method so that the frequency of emergence of mutants is smaller than the conventional methods. The inventive method may be generally applied even to the plants for which a regeneration method from a protoplast to a plant has not been established, and with which the preparation of the material to be subjected to the method is easy. That is, the present invention provides a method for transforming a monocotyledonous plant, comprising contacting a cultured tissue of said monocotyledonous plant during dedifferentiation thereof obtained by culturing an explant on a dedifferentiation-inducing medium for less than 7 days with a bacterium belonging to the genus Agrobacterium containing a super binary vector having the virulence region of a Ti plasmid, left and right border sequences of T-DNA of a Ti plasmid or an Ri plasmid of a bacterium belonging to the genus Agrobacterium, and a desired gene located between said left and right border sequences.
Owner:METABOLIX

Mono-active center Ziegler-Natta catalyst for olefinic polymerization

ActiveCN1884311AEasy to prepareHigh activity copolymerizationAluminoxaneZiegler–Natta catalyst
The invention relates to a new catalyst for single active central Ziegler-Natta alkene polymerization. Said catalyst takes salicylal containing dentate or substituted salicylal derivatives as electrons, and is prepared by adding pretreated carrier, metallic compound and electrons into magnesium compound / tetrahydrofuran solution. The catalyst can produce ethane homopolymer and copolymer with narrow molecular weight distribution (1.6-3.8) and even comonomer distribution, with high activity and under action of adjuvant catalyst of alkyl aluminium and alkyl aluminoxanes. The ethane polymerization, homopolymerization or combined polymerization of ethane and 1- olefin, ring olefin and polar monomer through slurry method or gas phase method by using said catalyst can be realized.
Owner:SHANGHAI INST OF ORGANIC CHEMISTRY - CHINESE ACAD OF SCI

Method for Making Multispecific Antibodies Having Heteromultimeric and Common Components

The invention relates to a method of preparing heteromultimeric polypeptides such as bispecific antibodies, bispecific immunoadhesins and antibody-immunoadhesin chimeras. The invention also relates to the heteromultimers prepared using the method. Generally, the method provides a multispecific antibody having a common light chain associated with each heteromeric polypeptide having an antibody binding domain. Additionally the method further involves introducing into the multispecific antibody a specific and complementary interaction at the interface of a first polypeptide and the interface of a second polypeptide, so as to promote heteromultimer formation and hinder homomultimer formation; and / or a free thiol-containing residue at the interface of a first polypeptide and a corresponding free thiol-containing residue in the interface of a second polypeptide, such that a non-naturally occurring disulfide bond is formed between the first and second polypeptide. The method allows for the enhanced formation of the desired heteromultimer relative to undesired heteromultimers and homomultimers.
Owner:GENENTECH INC
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