3670results about How to "Effective isolation" patented technology

The present invention relates to a nuclear reprogramming factor having an action of reprogramming a differentiated somatic cell to derive an induced pluripotent stem (iPS) cell. The present invention also relates to the aforementioned iPS cells, methods of generating and maintaining iPS cells, and methods of using iPS cells, including screening and testing methods as well as methods of stem cell therapy. The present invention also relates to somatic cells derived by inducing differentiation of the aforementioned iPS cells.

Provided is a batch-type Atomic Layer Deposition (ALD) apparatus for performing ALD processing collectively for a plurality of substrates, leading to an improved throughput, and achieving perfect uniformity of ALD on the substrates. The batch-type ALD apparatus includes: a chamber that can be kept in a vacuum state; a substrate support member, disposed in the chamber, supporting a plurality of substrates to be stacked one onto another with a predetermined pitch; a substrate movement device moving the substrate support member upward or downward; a gas spray device continuously spraying a gas in a direction parallel to the extending direction of each of the substrates stacked in the substrate support member; and a gas discharge device, disposed in an opposite side of the chamber to the gas spray device, sucking and evacuating the gas sprayed from the gas spray device.

The present invention refers to synthetic antibody molecules which comprise domains from naturally occuring antibodies, e.g. domains derivable from IgG, preferably of human origin, in a novel arrangement. Single chain molecules are provided which are suitable for expression in micro-organisms in their active conformation, which single chain molecules generally comprise a VL domain, a CL domain, and a VH domain, a CH1 domain, linked by a linker arranged between VUCL and VH/CH1. Accordingly, these antibody molecules can be termed single chain Fabs (scFabs). These antibody molecules are single chain proteins, which can also be associated to dimers, including heteromeric antibodies, wherein at least two single chain antibody molecules are associated.

The present invention concerns the use of methods and compositions for the isolation of small RNA molecules (100 nucleotides or fewer), such as microRNA and siRNA molecules. Such molecules are routinely lost in commonly used isolation procedures and therefore the present invention allows for a much higher level of enrichment or isolation of these small RNA molecules.

The present invention relates to a nuclear reprogramming factor having an action of reprogramming a differentiated somatic cell to derive an induced pluripotent stem (iPS) cell. The present invention also relates to the aforementioned iPS cells, methods of generating and maintaining iPS cells, and methods of using iPS cells, including screening and testing methods as well as methods of stem cell therapy. The present invention also relates to somatic cells derived by inducing differentiation of the aforementioned iPS cells.

Techniques for protecting content to ensure its use in a trusted environment are disclosed. The stored content is protected against harmful and/or defective host (or hosted) environments. A trusted security component provided for a device can verify the internal integrity of the stored content and the host before it allows the content to come in contact with the host. As a counter part, a trusted security component provided for the host can verify and attest to the integrity of the host and/or specific host computing environment that can be provided for the content stored in the device. The trusted security component provided for a device effectively verify the host integrity based on the information attested to by the trusted security component provided for the host. If the trusted security component trusts the host, it allows the trusted host to provide a trusted host computing environment trusted to be safe for the content stored in the device. A trusted host can effectively provide a safe virtual environment that allows a content representing a copy (or image) of an original computing environment to operate on the host computing system to give a similar appearance as the original computing environment.

The present invention relates to a reaction system whereby a peptide produced in an in vitro peptide synthesis system can be efficiently isolated at a high purity from the reaction system. Thus the present invention is a process for producing a peptide or a peptide derivative by using a reaction system of transcribing a DNA into an RNA and then translating the RNA produced or a reaction system of translating an RNA in vitro wherein at least one protein component of the reaction system is labeled with a first substance which adheres to a second substance, and said second substance is used as an adsorbent for capturing said labeled protein components after translating.

The present invention provides for an improved method and system for determining differences in data sets or data files, expedited data transfer and data reconciliation in a communication network using hash segmentation processing. The system and method provides for an efficient means of communicating updated files, new revisions or verifying files between a source host and a target host. By implementing hash segmentation processing, and in many embodiments iterative hash segmentation processing, the updates within the files can be isolated for the purpose of minimizing the amount of data communicated from the source host to the target host. The system and methods provide for the transfer of data between two hosts in instances in which neither host is aware of the revision that exists on the other host. The hash segmentation process may implement a logarithmic hash approach or a sliding linear hash approach.

A method and device for determining the transmuriality and/or continuity of an isolation line formed by a plurality of point contact ablations. In one embodiment, a method for determining the size of a lesion (width, depth and/or volume) is disclosed, based on contact force of the ablation head with the target tissue, and an energization parameter that quantifies the energy delivered to the target tissue during the duration time of the lesion formation. In another embodiment, the sequential nature (sequence in time and space) of the ablation line formation is tracked and quantified in a quantity herein referred to as the “jump index,” and used in conjunction with the lesion size information to determine the probability of a gap later forming in the isolation line.

Techniques for managing and protecting computing environments are disclosed. A safe computing environment can be provided for ensuring the safety and/or management of a device. The safe computing environment can be secured by a safe component that isolates and protects it from unsafe computing environments which may also be operating. As a result, various security and management activities can be securely performed from a safe computing environment. A safe computing environment can, for example, be provided on a device as a safe virtual computing environment (e.g., a safe virtual machine) protected by a safe virtual computing monitor (e.g., a safe virtual machine monitor) from one or more other virtual computing environments that are not known or not believed to be safe for the device. It will also be appreciated that the safe components can, for example, be provided as trusted components for a device. As such, various trusted components (or agent) can operate in a trusted computing environment secured from interference by components that many not be trusted and perform various security and/or management tasks alone or in connection, for example, with other trusted components (e.g., trusted serves).

A system for manipulating a planar substrate such as a semiconductor wafer is provided. The manipulator is typically used in conjunction with an XY stage to focus and planarize a wafer with respect to a tool. The manipulator employs redundant actuators of different types and a control system that uses low-bandwidth, high efficiency actuators to provide low frequency forces and high-bandwidth, but less efficient, actuators to provide all other forces. The manipulator provides support and manipulation of a substrate while minimizing errors due to thermal distortion.

A finished flooring underlayment is disclosed including a flexible membrane which is resistant to water, mold and deterioration under wet or dry conditions, and which has intertwined filaments bonded to the membrane surface that act as mortar reinforcement and as gauges to provide a uniform thickness of application for cementicious mortar. The mortar is provided separately and is mixed to a suitable consistency with either water or latex additive, then poured or placed on the mat, utilizing the gauges provided by the filaments to effect a uniform thickness of application. This mortar hardens to a strong, load bearing surface or slab suitable for application of finished flooring.

The invention discloses a system and a method for managing a virtual network in a cloud computation data center, and relates to the technical field of network system structures and network communication protocols. The system comprises physical servers and a controller, the physical servers are connected with a core switching network of the data center, each physical server is provided with at least one switch supporting OpenFlow related protocols and is connected with the controller, and the controller builds the virtual network, maintains configuration of the virtual network and a mapping relation among the virtual network, a virtual broadcast domain and a local broadcast domain, and configures and controls the switches of the physical servers. By the aid of the system and the method, pressure on broadcasting and double-layer address tables and forwarding tables of the core switching network of the data center can be effectively reduced, large-scale management is facilitated, flows of different users can be effectively isolated, security is strengthened, and configuration of the virtual network of the data center can be quickly and flexibly changed along with dynamic distribution of virtual resources.

The invention discloses a seedling planting method in an alkaline land. The method comprises the steps of: digging planting pits, laying a salt and alkali isolating layer, laying a water storage layer, laying a percolation layer, backfilling planting soil and cultivating seedlings. By adopting the seedling planting method in the alkaline land disclosed by the invention, the salt and alkali are effectively isolated, drought defying and waterlogging control are realized, the survival rate of the seedling is improved, and the normal growth of the seedlings is ensured; large-area consolidation of the alkaline land is not needed, and only the local plot, in which the seedlings are planted, is needed to be consolidated, so that the seedlings can be planted; and the method is simple, feasible and worthy of popularizing.

The invention comprises compositions and methods useful for encapsulation and controlled release of guest molecules, such as drugs. Compositions of the present invention comprise a matrix comprising molecules that are non-covalently crosslinked by multi-valent cations, wherein the molecules that are non-covalently crosslinked are non-polymeric, have more than one carboxy functional group, and have at least partial aromatic or heteroaromatic character The compositions are characterized in that a guest molecule may be encapsulated within the matrix and subsequently released.

A membrane applied to the ostium of an atrial appendage for blocking blood from entering the atrial appendage which can form blood clots therein is disclosed. The membrane also prevents blood clots in the atrial appendage from escaping therefrom and entering the blood stream which can result in a blocked blood vessel, leading to strokes and heart attacks. The membranes are percutaneously installed in patients experiencing atrial fibrillations and other heart conditions where thrombosis may form in the atrial appendages. A variety of means for securing the membranes in place are disclosed. The membranes may be held in place over the ostium of the atrial appendage or fill the inside of the atrial appendage. The means for holding the membranes in place over the ostium of the atrial appendages include prongs, stents, anchors with tethers or springs, disks with tethers or springs, umbrellas, spiral springs filling the atrial appendages, and adhesives. After the membrane is in place a filler substance may be added inside the atrial appendage to reduce the volume, help seal the membrane against the ostium or clot the blood in the atrial appendage. The membranes may have anticoagulants to help prevent thrombosis. The membranes be porous such that endothelial cells cover the membrane presenting a living membrane wall to prevent thrombosis. The membranes may have means to center the membranes over the ostium. Sensors may be attached to the membrane to provide information about the patient.

The present invention relates to a process for producing CaCO3 or MgCO3 from a feedstock comprising a Ca- or Mg-comprising mixed metal oxide, wherein: (a) an aqueous slurry of the feedstock is contacted with a C02 containing gas to form an aqueous solution of Ca(HCO3)2 or Mg(HCO3)2 and a solid Ca- or Mg-depleted feedstock; (b) part or all of the aqueous solution of Ca(HCO3)2 or Mg(HCO3)2 is separated from the solid Ca- or Mg-depleted feedstock; (c) CaCO3 or MgCO3 is precipitated from the separated aqueous solution of Ca(HCO3)2 or Mg(HCO3)2; and (d) the precipitated CaCO3 or MgCO3 is recovered as product. The invention further relates to a process for the production of an aqueous solution of Ca(HCO3)2 or Mg(HCO3)2.

Spheroidal beads present an exterior surface of a hydrophilic hydrogel, which is an isocyanate-functional polymer that is polymerized by urethane bonds and cross-linked by urethane and urea bonds. Sequestering agents present at the surface are covalently bound to isocyanate groups or to intermediate linkers that are so bound. These beads allow sequestering agents to retain their native three-dimensional configuration, and as a result of such surface characteristics and hydrophilicity, they achieve highly effective capture of very small subpopulations of rare cells from bodily fluids or the like and very effectively deter nonspecific binding of other biomaterials present in such bodily fluid. They may be all-hydrogel spheroids or hydrogel-coated substrates.

The invention relates to an olefin two-phase hydroformylation method, which consists of three parts: polyether guanidine mesylate ionic liquid (PGMILs) with room temperature solidifiable characteristics, complex catalysts (Rh-TPPTS) formed by RhCl3.3H2O or dicarbonylacetylacetonato rhodium and triphenylphosphine sodium trithionate (TPPTS), and reactants of C6-C14 straight chain 1-olefin, wherein the Rh-TPPTS is dissolved in the PGMILs to form a lower layer catalyst phase, the C6-C14 straight chain 1-olefin or product aldehyde forms an upper layer organic phase, the selectivity of high-carbon aldehyde is 85 to 99 percent, the mol ratio of normal aldehyde to isomerism aldehyde is 2.0 to 2.4, the PGMILs phase containing Rh-TPPTS can be cyclically used for 35 times, the activity and the selectivity are unchanged, the accumulated conversion number (TON) reaches higher than 30000, rhodium flowing to the product phase is 0.04 percent to 0.07 percent, and ultra-long-period catalysis activity and selectivity can be realized.

A medical device, such as a respiratory treatment device, includes a passive acoustic noise reduction system in the form of a vibration reducing blower assembly isolation mount. The device includes a housing, a blower assembly mounted therein, a patient circuit coupled to the blower assembly, and a noise reducing blower assembly isolation mount. The mount includes a first vibration damping member adjacent one side of the blower assembly spacing the blower assembly from a mounting surface upon which the blower assembly is supported. A securing member positions and holds the blower assembly onto the mounting surface, and a second vibration damping member adjacent an opposite side of the blower assembly spaces the blower assembly from the securing member. The vibration damping members are injection molded thermoplastic elastomeric members.

Anthocyanins, other flavonoids and related polyphenolic compounds are extracted from fruits or highly pigmented garden vegetables and their by-products using subcritical water with or without a secondary cosolvent. This method has the advantages of being relatively inexpensive, minimizes or totally avoids the use of objectionable solvents, and provides a facile means for supplying an ample source of concentrated phytochemicals for use in food formulation, dietary supplements and phyto-pharmaceutical applications.