74 results about "Small RNA" patented technology

The invention belongs to the field of biological detection, and discloses an exosome RNA detection composition based on positive and negative targets and a use method thereof, the composition comprises two positive targets and one negative target: the positive target A is miRNA stably expressed in exosome and is used for reflecting the expression level of small RNA; the positive target B is typical mRNA and is used for evaluating the quality of the long-fragment RNA; the negative target C is bacterial 16S rRNA and is used for identifying bacterial nucleic acid pollution in a sample. The method combines III-generation reverse transcriptase and HotStart Taq enzyme to construct a qPCR system, has high sensitivity and specificity, and is suitable for rapid detection of trace RNA. Whether the exosome RNA is suitable for downstream analysis or not can be judged by acquiring the Cq values of the three types of targets and setting a reference range. According to the invention, the integration of quality control, quantification and pollution identification of the exosome RNA is realized.

The invention belongs to the technical field of gene engineering, and particularly relates to application of corn zma-miR319 and / or a target gene of the corn zma-miR319 in regulation and control of corn stem rot resistance. According to the application, sequencing data of small RNA, a degradation group and a transcriptome are integrated, a miRNA family member zma-miR319 is identified, and the resistance of corn to stem rot can be positively regulated and enhanced; the target gene ZmMYB74 is a core regulation gene of an Fg response module, the stem rot resistance of a transgenic plant over-expressing the ZmMYB74 is weakened, and lignin deposition is reduced; the resistance is obviously enhanced by knocking out or inhibiting ZmMYB74, the ZmMYB74 is used as a transcription inhibition factor, the expression of a lignin synthesis related gene ZmCAD is negatively regulated and controlled, and the separation of the disease-resistant gene ZmMYB74 not only contributes to the cultivation of a persistent disease-resistant corn variety, but also deepens the understanding of a stem rot resistance molecular mechanism.

The invention discloses a novel tRNA (transfer ribonucleic acid) derived small RNA biomarker tRF5-23-GlyTCC-2 and application thereof, and relates to the technical field of biological medicines. The nucleotide sequence of the biomarker tRF5-23-GlyTCC-2 disclosed by the invention is as shown in SEQ ID NO. 1, and the nucleotide sequence of the biomarker tRF5-23-GlyTCC-2 is as shown in SEQ ID NO. Results of embodiments show that the gene has significant low expression in colorectal cancer tissues and serum, when the gene is independently used for colorectal cancer diagnosis, AUC for distinguishing a patient from a healthy control reaches 0.8628, the sensitivity is 74%, the specificity is 92%, and the AUC, the sensitivity and the specificity are both superior to CEA; the AUC is improved to 0.9077 and the sensitivity reaches 90% when the compound is used in combination with CEA (Carcino Embryonic Acid). Meanwhile, low expression of the marker is related to poor prognosis characteristics such as late T staging and low differentiation of a patient, and the marker can be used for prognosis evaluation. Functional experiments prove that overexpression can inhibit colorectal cancer cell proliferation and tumor growth, and a new scheme is provided for colorectal cancer diagnosis and treatment.

The application discloses two kinds of small RNA compositions for improving biological control effect of termites, and microRNA-like RNA (milRNA) sequences are milR-32 and milR-48 respectively. Double-stranded RNA can enhance the effect of the beetle green fungus on killing termites. The milR-32 and the milR-48 belong to the milRNA coded by the green fungus, and the gene sequences are derived from the transcriptome database of the beetle green fungus. The milR-32 and the milR-48 mimics are designed and synthesized based on the milR-32 and the milR-48 sequences respectively. After the beetle green fungus is used to infect the termites taking the milR-32 or the milR-48 mimic, the infection mortality of the termites is significantly increased. The mimic designed based on the milR-32 and the milR-48 sequences can significantly improve the biological control effect of the termites.

This disclosure relates to the field of nucleic acid drug technologies, and in particular to a small RNA-based drug, preparation, and use thereof in prophylaxis and / or treatment of cardiomyopathy. Provided is a novel small RNA with the nucleotide sequence of SEQ ID NO: 10. The small RNA exhibits good structural stability, marked efficacy at 24 h, and high affinity to nascent polypeptide-associated complex α (NACα) protein. With strong activity to suppress the expression of pyroptosis-related proteins, apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) and gasdermin D (GSDMD), the small RNA can effectively protect cardiomyocytes and enhance the therapeutic effect on diabetic cardiomyopathy (DCM). Furthermore, the small RNA posses advantages of low effective concentration and high biosafety, highlighting its potential for clinical drug development and application as a nucleic acid drug.

According to the invention, reverse transcriptase Marathon or a mutant thereof is fused with Cas nuclease, a small RNA binding exonuclease protection factor La and a positioning signal peptide to form a fusion protein capable of guiding editing. Experiments show that a guide editing system based on the fusion protein can realize base replacement operation in rice callus, and has important application value in the field of biological breeding.

The application belongs to the technical field of nucleic acid drugs, and particularly relates to a small fragment RNA derived from circular RNA and application of the small fragment RNA in treatment of diabetic vascular diseases. The nucleotide sequence of the small fragment RNA is CUAGCCGAAUGUUAAAAAAU. The small fragment RNA provided by the application has the effect of protecting and maintaining the biological function of vascular endothelial cells, has good structural stability, has a long half-life in the body, has the advantages of low effective concentration and high biological safety, has good application potential in the field of clinical nucleic acid drug development, and provides a new idea for treatment of chronic and progressive vascular lesions caused by diabetes.

The invention discloses a multi-omics plant phenotype prediction method fused with small RNA, and relates to the field of bioinformatics and agricultural biology, and the method comprises the following steps: obtaining and preprocessing genotype, transcript expression and small RNA expression data to obtain a standardized feature matrix; performing feature screening based on Pearson correlation on the standardized feature matrix to obtain corresponding genotype, transcript expression and small RNA expression feature subsets; and based on a pre-trained MirGP prediction model, through multi-branch feature extraction, multistage efficient channel attention fusion and regression prediction, outputting a plant phenotype prediction result. According to the method, multiple types of small RNA regulation and control features are introduced and a hierarchical deep learning fusion architecture is constructed, so that multiple omics feature dimensions are expanded, data redundancy is reduced, and effective integration and deep mining of cross-modal features are realized.

The application belongs to the field of pharmaceutical chemical industry and relates to a cyclic peptide compound shown as formula M or a stereoisomer, a tautomer or a mixture thereof of the compound, a pharmaceutically acceptable salt, a polymorph, a co-crystal or a solvate of the compound, or a stable isotope derivative, a metabolite or a prodrug of the compound. The application also relates to a method for synthesizing the cyclic peptide compound and an intermediate. The provided cyclic peptide compound has a strong inhibitory effect on a coronavirus 3CL protease and a small RNA virus (for example, an enterovirus EV71 virus) 3C protease. After cell level activity testing, the provided cyclic peptide compound has an obvious inhibitory effect on EV71 virus and coronavirus and the like, indicating that the cyclic peptide compound shown as formula M can produce a viral inhibitory effect at the cell level by inhibiting viral protease activity, and has a good application prospect as an antiviral drug.

The present invention relates to a method classifying target RNA modifications in a neural network system, to a method for training a neural network system that is configured to classify target RNA modifications, to a neural network system, to a computer system adapted to classify target RNA modifications, to a computer program and to a non-transitory readable medium.

This invention belongs to the field of plant molecular biology, specifically relating to a small RNA molecule that alleviates lignification of loquat fruit and its application. The small RNA molecule is from loquat. Eja-MIR164 Its nucleotide sequence is shown in SEQ ID NO. 6. After processing within the plant, it forms a functional mature form. This invention experimentally confirms that loquat fruit contains… Eja-MIR164 The expression level of Eja-miR164 was negatively correlated with lignin content; transient interference or stable transformation using STTM technology reduced Eja-miR164 content, targeting its genes NAC transcription factor and lignin polymerase gene. EjLAC7 Increased expression levels in all samples led to a significant increase in lignin content. This invention utilizes small RNA molecules. Eja- MIR164 It can be used to breed new loquat varieties with reduced lignification, improve fruit texture and quality, and enhance post-harvest storage performance, and has potential application value in loquat genetic improvement and molecular breeding.

The application relates to an anti-apoptosis baculovirus expression vector, which realizes a broad-spectrum anti-apoptosis effect by expressing siRNA targeting a Sf-caspase-1 and Tn-caspase-1 common sequence of Spodoptera frugiperda and Trichoplusia ni insect cells through the vector. The baculovirus vector contains a specific DNA sequence, the DNA sequence contains an siRNA sequence targeting the Sf-caspase-1 and Tn-caspase-1 common sequence transcribed by an RNA polymerase III promoter; the recombinant virus of the vector can express double-stranded small RNA in a host cell, silences the caspase-1 encoded by the host cell through an RNA interference pathway, thereby inhibiting the apoptosis of the host cell, and significantly improving the expression level of an exogenous protein. The application can be used for industrialized production of protein preparations and vaccines.

The invention discloses a method for chimeric expression of recombinant small RNA (Ribonucleic Acid) in escherichia coli by utilizing active tRNA (Ribonucleic Acid) and application, and the method is characterized in that target small RNA is expressed in escherichia coli by taking an active tRNA chimeric functional miRNA precursor as a bracket. In addition, the invention also discloses application of the recombinant small RNA produced by the method in preparation of a diagnostic reagent, a scientific research reagent, a prodrug, a drug, a bulk drug or a pharmaceutical composition. According to the invention, the human tRNA is used as the stent, so that the toxicity and immunogenicity are possibly lower; in addition, the active tRNA is used as a bracket, so that the effect of targeted treatment of diseases can be achieved, and a synergistic interaction effect can be achieved with carried small RNA. The cell toxicity is reduced, the expression quantity is improved, and a tRNA scaffold with activity is screened, so that the tRNA scaffold and chimeric small RNA synergistically exert a treatment effect.

This determination method comprises: determining whether specimen data including small RNA expression data as feature values contains a missing item with respect to feature values other than the small RNA expression data; if the specimen data includes a missing item, imputating the missing value of the specimen data on the basis of a missing value imputation model trained on the relationship between an index item and feature values included in the specimen data; and inputting the specimen data in which the missing value has been imputated to a determination model that determines a disease based on the small RNA expression data and feature values other than the small RNA expression data, thereby determining a disease of a subject associated with the specimen data.

The invention discloses citrus small RNA (Ribonucleic Acid) CitmiRn01 and application thereof in reducing the polyembryonic level and improving the zygotic embryo rate, and belongs to the technical field of plant molecular biological breeding. The invention finds that the citrus CitmiRn01 is a key small RNA (Ribonucleic Acid) for regulating and controlling a citrus nucellar embryo, the nucleotide sequence of the citrus CitmiRn01 is shown as SEQ ID NO.3, the precursor nucleotide sequence of the citrus CitmiRn01 is shown as SEQ ID NO.1 or 2, and overexpression of the CitmiRn01 can obviously reduce the multiple embryo level of the citrus, so that the CitmiRn01 can be used as an important candidate gene for cultivating a low multiple embryo level citrus variety. The invention provides important theoretical basis and gene resources for regulating and controlling the citrus polyembryonic level and widening the selection range of citrus crossbreeding female parents.

Provided is a small RNA, a composition comprising the small RNA, a method for using same and use of same. The small RNA or composition can inhibit the ability of any one or more of pathways or genes listed in Table 3, or decrease or down regulate the expression level of IL-1 beta, IL-6, and / or TNF-alpha in vitro or in vivo, or treat or prevent IL-1 beta, IL-6, and / or TNF-alpha related diseases and / or increase cell viability in a subject.

The invention belongs to the technical field of biology, and particularly relates to siRNA origami for reducing dsRNA off-target effect and application of the siRNA origami in pest control. In order to provide a solution integrating an efficient low-off-target siRNA sequence, a self-assembled and stable nano delivery structure and pest control application, the siRNA sequence is obtained through small RNA sequencing screening on the basis of a dsRNA sequence, and has high RNAi activity and low off-target risk. An auxiliary chain is introduced, and a double-chain intermediate with a three-way connection structure and three pairs of kiss rings as structural cores is formed through self-assembly according to the base complementary pairing principle; the intermediate can be further efficiently self-assembled into tetrahedral RNA origami nanoparticles with a stable structure through kiss-ring pairing. According to the nanoparticles, the stability and the cell uptake efficiency can be remarkably improved, efficient and high-specificity gene silencing is finally achieved in pest bodies, and the off-target effect of traditional dsRNA is effectively reduced.

The application discloses a method for detecting viruses and virus replication in media based on Small-RNA high-throughput sequencing. The application belongs to the technical field of virus detection, and the method for identifying virus types in a to-be-detected sample by Small-RNA sequencing developed by the application comprises the following steps: 1) extracting total RNA from a virus-infected to-be-detected sample, constructing a cDNA library, and separating small RNA sequence fragments from the cDNA library; 2) downloading virus sequences and classification databases from GenBank, and comparing the small RNA sequence fragments with virus sequence files; 3) comparing a contigs3 file with a virus nucleic acid database, selecting sequences with the minimum e value in the comparison results in the contigs3 file, calculating the coverage of the contigs3 sequences in corresponding virus genomes, distinguishing virus information with a coverage threshold, and deducing the virus source of the small RNA sequence.

This invention relates to the application of salicylamide derivatives in the preparation of anti-small RNA virus drugs, belonging to the field of antiviral drug technology. This invention discloses the application of salicylamide and / or its derivatives in the preparation of anti-small RNA virus drugs. Through various experimental methods, this invention confirms that niclosamide and its derivatives HJW1107 and CZY1111 can significantly inhibit the early infection and replication of small RNA viruses such as EMCV, EV-D68, and CVA10, inhibit virus-induced cytopathic effects, and enhance the survival rate of infected cells. This indicates that niclosamide and its derivatives HJW1107 and CZY1111 have broad-spectrum antiviral activity and can be used to prepare therapeutic drugs against small RNA virus infections, thereby preventing and treating diseases caused by small RNA viruses, and has good clinical application prospects.

The present application discloses a marker determination method, device, equipment, storage medium and program product. The method comprises: acquiring first expression quantity and second expression quantity of multiple target objects in multiple microRNAs in the perioperative period; determining a first microRNA from the multiple microRNAs according to the first expression quantity, the second expression quantity and a change quantity; inputting the first expression quantity and the second expression quantity into a preset screening model to obtain a second microRNA by screening; for each target object, drawing an expression quantity change trajectory curve of each microRNA of the target object according to the first expression quantity and the second expression quantity; determining a third microRNA according to the expression quantity change trajectory curve; and determining a first candidate target marker according to the first microRNA, the second microRNA and the third microRNA. According to the embodiments of the present application, the long-term clinical events of coronary heart disease are accurately predicted through the change of the determined marker.

The present disclosure relates to a single stranded RNA vector suitable for introducing a therapeutic agent, such as a peptide, a protein or a small RNA, into a host plant. The vector does not encode for any movement protein or coat protein, but is capable of capable of systemic and phloem-limited movement and replication within the host plant.

The invention discloses a corn pathogen early warning method and system based on big data, and relates to the technical field of agricultural early warning, the method comprises the following steps: comparing sequences in historical small RNA deep sequencing data and verified viral pathogen monitoring data, and establishing a mapping relationship between the two to obtain a pathogen-small RNA reference corresponding relationship library; matching the small RNA deep sequencing data of the current monitoring period with the pathogen-small RNA reference corresponding relationship library, and extracting sequence fragments consistent with the mapping relationship in the library to obtain a target viral pathogen feature sequence; coupling the target viral pathogen feature sequence with the current environmental factor data; according to the method, the core value in the multi-source data can be effectively mined, the early infection signal of the pathogen can be accurately captured, the early accurate prediction of the transmission risk and potential outbreak of the corn viral pathogen is realized, a timely and reliable decision basis is provided for field prevention and control, and the corn production safety is guaranteed.

The invention discloses a dendrobe small RNA (Ribonucleic Acid) miR421 and an application thereof. According to the application of the miR421 in regulation and control of synthesis of the plant polysaccharide, the mature sequence of the miR421 is as shown in SEQ ID NO. 1. According to the invention, miR421 belonging to dendrobium is cloned from dendrobium officinale leaves, the miR421 is used as a negative regulatory factor to participate in regulating the synthesis process of dendrobium polysaccharide, and transient expression and functional identification prove that the polysaccharide content of transgenic dendrobium is reduced when the miR421 is excessively expressed. Therefore, the miR421 has important theoretical guidance significance and application value in the fields of synthesis of secondary metabolites of orchid plants and medical application.

A model substance according to the present disclosure comprises a mixture prepared by mixing biological samples collected from eight or more subjects of the same biological species and can be used for quantitative analysis of small RNA.

The application provides a small RNA detection method and application thereof. The application discloses a method for detecting 3' end modification of sRNA, including a TANT-seq method and a TE-qPCR method. The detection method disclosed by the application provides a new technical means for sRNA-cP detection and identification, and also provides a new idea for one-time qualitative / semi-quantitative / quantitative identification of various terminal modified sRNAs.

A composition for treating cancer is disclosed. The composition includes a lentiviral particle and an aminobisphosphonate drug. The lentiviral particle is capable of infecting a target cell, such as a cancer cell, and includes an envelope protein optimized for targeting such target cell and a viral vector. The viral vector includes a small RNA optimized to target an FDPS mRNA sequence. The aminobisphosphonate drug includes zoledronic acid.

A non-resistance plasmid production system constructs a plasmid containing a nucleic acid fragment 3 for coding small RNA, a host cell expression cassette containing a toxic gene expression cassette and an anti-toxic gene expression cassette through combined design of a toxic transcript / anti-toxic transcript, an operon related element and the small RNA. The toxic gene expression cassette comprises a promoter and a nucleic acid fragment 1 for transcribing a toxic transcript, and the anti-toxic gene expression cassette comprises a promoter, an operon and a nucleic acid fragment 2 for transcribing an anti-toxic transcript. Through reverse complementation of the nucleic acid fragment 3 and the nucleic acid fragment 1, a steric effect is generated, and a toxic transcript is inhibited, so that the screening production of plasmids can be realized without adding antibiotics or other inhibitors in the whole plasmid production process, and the harm of the antibiotics and the inhibitors to the plasmids in cell gene therapy is solved.

The application discloses a kind of small RNA fusion multiomics plant phenotype prediction method, it is related to bioinformatics and agricultural biology field, including the following steps: obtaining and pre-processing genotype, transcript expression and small RNA expression data, obtain standardized feature matrix;The standardized feature matrix is screened based on the feature of pearson correlation, and the corresponding genotype, transcript expression, small RNA expression feature subset is obtained;Based on the pre-trained MirGP prediction model, by multi-branch feature extraction, multi-level efficient channel attention fusion and regression prediction, output plant phenotype prediction result.The method introduces multiple small RNA regulation characteristics and constructs hierarchical deep learning fusion architecture, expands the multiomics feature dimension, reduces data redundancy, realizes the effective integration and deep mining of cross-modal features.

The present disclosure relates to a viral vector suitable for introducing a therapeutic agent, such as a peptide, protein or small RNA, into a host or otherwise treating a host. The vector may include an exogenous RNA segment with a hairpin-like structure or having two or more base-paired regions separated by one or more non-base-paired regions. The exogenous RNA segment may have a secondary structure, minimum free energy, average positional entropy or other attributes within specified ranges, or with values similar to one or more hairpin-like structures of a reference wild type virus. Optionally, the RNA vector may be derived from the reference wild type virus or a relative of the reference wild type virus. In some examples, the vector is capable of capable of systemic and phloem-limited movement and replication within a host plant. In some examples, the reference wild type virus is an umbravirus-like associated RNA.