The invention provides application of fructus arctii
essential oil in preparation of an external
medicine for resisting
human papilloma virus. The invention further provides application of the
ganoderma lucidum
spore oil in preparation of the external
medicine for resisting the
human papilloma virus. The invention provides a pharmaceutical composition for treating
human papilloma virus infection. The pharmaceutical composition is prepared from the following raw materials in parts by weight: 3.2-4.8 parts of fructus arctii
essential oil and 0.8-1.2 parts of
ganoderma lucidum
spore oil. The invention also provides a preparation method and application of the pharmaceutical composition. According to the invention, a compounding process is adopted, the formula of main medicines and auxiliary materials is determined, the compound
ganoderma lucidum
essential oil with good stability is successfully prepared, and a thin-layer
chromatography method and a
gas chromatography quality evaluation method for the main medicines in the compound are preliminarily established. The compound
coriolus versicolor essential oil is administrated in a local smearing mode, in-vitro experiments show that the compound
coriolus versicolor essential
oil can interfere with
virus replication and is high in antiviral effect, especially for HPV16, the effect is mild, no stimulation to
skin is found through clinical observation, and adverse reactions are few.