siRNA disturbing rdrp gene function of SARS virus

A technology of SARS virus and RNA polymerase, applied in the field of siRNA, can solve the problems of high efficiency, high price, low price, etc., and achieve the effects of avoiding gene suppression, overcoming high price, and having remarkable effect.

Inactive Publication Date: 2008-01-30
SICHUAN UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0008] siRNA is generally obtained by the following methods: 1) Chemical synthesis: the synthetic siRNA has high purity and unlimited synthetic quantity, but is expensive
2) "cocktail" (cocktail) method: it can quickly and economically study the phenotype of a gene function loss, but because the siRNA used is a mixture, the effective siRNA target sequence cannot be determined, and non-specific gene inhibition may occur
3) In vitro transcription synthesis method: low toxicity, good stability, high efficiency, and low price. The disadvantage is that the amount of siRNA synthesis is limited

Method used

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  • siRNA disturbing rdrp gene function of SARS virus
  • siRNA disturbing rdrp gene function of SARS virus
  • siRNA disturbing rdrp gene function of SARS virus

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Embodiment Construction

[0060] 1. Assembly of SARS virus RdRp gene

[0061] Due to the conservation of sequence and the importance of function, the RdRp gene of SARS virus has become an important breakthrough to reveal the secrets of the virus, and it has also become an ideal target for research and development of SARS virus-specific drugs and vaccines. But because of its high infectivity, the virus is tightly controlled and out of reach of most researchers. At the same time, even if the virus is obtained, the experimental operation is still relatively risky. For further research, we need to design a safe, efficient and reliable method to obtain this gene.

[0062] DNA shuffling, also known as DNA reassembly, is an in vitro molecular evolution technique. It works by randomly dividing the parental DNA molecule into small fragments, and then randomly recombining these small fragments to obtain new molecules. During recombination, through complementary pairing of terminal sequences, these small fragm...

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Abstract

The invention discloses two anti-SARS-coV siRNA and applications thereof. The anti-SARS-coV siRNA of the invention is of obvious functional effect, and can be acquired through establishing siRNA expression box, which overcomes the problem of high price of chemical synthesized siRNA, and plays an important role in the prevention work of SARS-coV.

Description

technical field [0001] The present invention relates to two kinds of siRNA against SARS virus RNA-dependent RNA polymerase (RdRp) gene and application thereof. Background technique [0002] In 2002-2003, a highly contagious "atypical pneumonia" spread around the world. The disease first started in Guangdong Province, China, and gradually spread to all parts of China and even more than 30 countries on 5 continents in a horrific manner. A total of more than 8,000 patients were infected. In some places, the death rate of patients reached 3%-10%. . The disease has an acute onset, strong infectivity, and rapid spread, which seriously threatens the lives of patients. It usually has an incubation period of 2-12 days. The main clinical manifestations are high fever (>38°C), headache and dry cough. Some patients have shortness of breath and other symptoms of dyspnea. A small number of patients can progress to respiratory distress syndrome (ARDS) with a very high mortality rate. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H21/02C12N15/63A61K31/7088A61K48/00A61P31/14A61P11/00
Inventor 张义正王刚李珉王海燕冯红
Owner SICHUAN UNIV
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