396 results about "Viral gene" patented technology

The invention relates to the technical field of knowledge maps, in particular to a multi-task hepatitis B drug screening method and system based on knowledge map assistance, and the method comprises the following steps: based on hepatitis B virus genotype sequence data recorded along with time, patient drug use records and drug sensitivity. According to the method, virus genotype sequence data, patient drug use records, drug sensitivity and other multi-dimensional dynamic time sequence data are integrated, interaction events between entities are marked through timestamps, and virus variation tracks, drug curative effect changes and other dynamic characteristics are embedded into atlas node attributes; and the knowledge representation can reflect the time dependence in a real scene. A hyperedge connection multi-entity set is defined based on biological pathway annotation information and protein interaction data, the limitation that only a binary relation is supported in a traditional knowledge graph is expanded, a drug combination and multi-target synergistic effect mechanism is explicitly modeled, and misjudgment of the combination effect caused by simplification of the interaction relation is avoided.

The invention provides a virus propagation dynamic prediction method based on a multi-strain multi-region population model and related equipment. The method comprises the following steps: acquiring historical epidemic situation related data of a target area, wherein the historical epidemic situation related data comprises epidemic situation data, virus gene sequence data, population flow data and epidemic situation management policy data related to a target virus in a past time period of each area; arranging the population flow data into an asymmetric population flow matrix between different regions every day; sorting epidemic prevention grades corresponding to different epidemic situation management policies according to the epidemic situation management policy data; and constructing a multi-strain and multi-region population model based on epidemic situation data, virus gene sequence data, a population flow matrix and epidemic prevention grades corresponding to different epidemic situation management policies by taking StatPOMP as a model construction framework, and generating virus propagation dynamic prediction information based on the model. The prediction accuracy of the virus propagation trend can be improved.

The invention belongs to the technical field of virus detection, and discloses a digital PCR (Polymerase Chain Reaction) kit for simultaneously detecting African horse pestivirus, West Nile virus and equine influenza H3N8, and the kit comprises primers and probes as shown in SEQ ID NO.1-SEQ ID NO.21. The invention also discloses a kit for simultaneously detecting African horse pestivirus, West Nile virus and equine influenza H3N8. According to the invention, after a single system of an African horse pestivirus VP7 gene, an African horse pestivirus-NS2 gene, a west nile virus-NS5 gene, a west nile virus-NS2a gene, an equine influenza H3N8-HA gene and an equine influenza H3N8-NA gene is tested, a seven-fold digital PCR (Polymerase Chain Reaction) system containing an internal standard is established; through tests of indexes such as linearity, specificity, minimum detection limit, repeatability, comparison of a single digital PCR system and a multiple digital PCR system, the currently developed digital PCR multiple detection kit completely meets development requirements and is high in specificity and sensitivity, and the minimum detection limit of the digital PCR multiple detection kit can reach 10 Copies / mu L or below.

The invention provides a structure, a sequence and a construction method of a plant virus gene editing vector and application of the plant virus gene editing vector in plant gene editing. The method is characterized in that a TRV2 genome of a tobacco embrittlement virus (TRV) is used for carrying and expressing a small Cas gene AsCas12f and gRNA of the small Cas gene AsCas12f. Preferably, the AsCas12f and the gRNA of the AsCas12f are subjected to tandem expression by utilizing the TRV2 to form an expression cassette structure of the AsCas12f-tRNA-gRNA, the expression cassette substitutes a part of 2b gene sequence of the TRV2 on a TRV2 genome to form a plant gene editing vector pTRV2-Cas12f-gRNA based on the TRV virus, and long-time stable expression and gene editing can be performed after wild type nicotiana benthamiana is inoculated. The invention also provides a method for carrying out gene editing in a wild type plant by utilizing the plant virus.

The invention discloses a live pig epidemic prevention and control flow regulation system and method based on big data, and relates to the technical field of animal health information, and the method comprises the steps: recognizing abnormal live pig signals and track intersection features of structured epidemic prevention feature data through an intelligent algorithm, comparing the virus gene difference degree, constructing a virus propagation network diagram, and carrying out the analysis of the virus propagation network diagram; outputting a high-risk propagation node map; constructing an aerosol dynamics three-dimensional space model by taking a super propagation hub node of the high-risk propagation node map as an original point and combining culture environment parameters, simulating a virus air diffusion path, and generating an environment propagation thermal distribution map; fusing the high-risk propagation node map and the environment propagation thermal distribution map, identifying a cross infection area through a multi-dimensional risk superposition model to perform risk dynamic grading, and outputting a grading early warning report; according to the invention, breakthrough improvement of the live pig epidemic prevention and control capability is realized, key nodes and potential risk areas of epidemic propagation can be accurately identified, and the pertinence of prevention and control measures is improved.

The present invention relates to methods of producing microbubble drug conjugates, viral gene therapy microbubble drug conjugates, and disease-targeting microbubbles, for clinical and preclinical ultrasound-mediated therapeutic and diagnostic applications. It includes methods to produce viral vector gene therapy microbubble drug conjugates with antibody linkers conjugated to lipid shelled microbubbles that both bind to and neutralize viral vectors such that the viral gene therapy can transduce and effect permanent genetic changes only after ultrasound is used to disassociate the viral gene therapy from microbubbles at diseased regions of the body.

The invention provides a pseudovirus of a Nipah virus and application of the pseudovirus, and belongs to the technical field of genetic engineering. The invention provides a pseudovirus of a Nipah virus and application of the pseudovirus. The pseudovirus contains a nucleic acid fragment of a Nipah virus N gene of which the nucleotide sequence is shown as SEQ ID NO.1. The invention further provides a preparation method of the pseudovirus. The pseudovirus of the Nipah virus can be used as a ribonucleic acid standard substance of the pseudovirus of the Nipah virus, and is applied to the aspects of quantity transmission, calibration, instrument testing, evaluation and control of a testing method, quality verification, quality control, production process detection and the like of a laboratory related to Nipah virus detection. The pseudovirus of the Nipah virus, as the ribonucleic acid standard substance of the pseudovirus of the Nipah virus, has the following advantages of accurate fixed value, good uniformity, stable quantity value and traceability, and can promote the improvement of the detection capability and the technical level of a national Nipah detection laboratory.

The disclosure relates to block copolymer nanoparticles for therapeutic delivery of nucleic acids, and methods therefor. More particularly, the invention relates to polymer nanoparticles, such as reversible addition-fragmentation chain transfer (RAFT) polymer compositions, for delivering miRNAs.

The invention discloses a fiveleaf virus GI.2 whole genome sequencing primer based on an amplicon sequencing technology and application thereof, and belongs to the technical field of virus gene sequencing. The primer group composed of 18 pairs of primers is preferably obtained according to an amplicon sequencing technology, the primer group can effectively improve the primer amplicon coverage degree, it is guaranteed that the primer amplicon coverage degree is 1.5-1.8 times that of a virus sequence, the amplification time is shortened, the amplification difficulty is reduced, the primer amplicon coverage degree and the whole genome capture efficiency are improved, and the primer amplification efficiency is improved. And the method has important significance on epidemic situation traceability and propagation trend of the fiveleaf virus GI.2.

The invention discloses a polyclonal antibody of a grouper iridovirus SGIV VP101 gene and application of the polyclonal antibody. The polyclonal antibody is prepared from an SGIVVP101 gene, and the nucleotide sequence of the VP101 gene of an optimized codon is as shown in SEQ ID NO. 1. The polyclonal antibody of the SGIV VP101 gene recombinant expression protein prepared by the invention has good specificity, not only can specifically recognize a prokaryotic expression product of the SGIV VP101, but also can be used for quantitative and positioning analysis of the SGIV VP101 in vivo and in vitro. The successful preparation of the polyclonal antibody of the recombinant expression protein of the SGIV VP101 gene lays a foundation for the establishment of an SGIV immunological detection method, the quantitative and positioning analysis of the protein of the VP101 gene and the analysis of gene functions.

The invention belongs to the field of canine distemper virus detection, and particularly relates to a canine distemper virus RT-RAA CRISPR / Cas 12b detection kit and a detection method thereof. According to the kit, isothermal amplification is performed by using an RT-RAA universal primer, and then detection is performed by using a CRISPR / Cas 12b detection system consisting of target sequence sgRNA, so that amplification of a target fragment of the N gene of the canine distemper virus and target detection visualization can be effectively realized. The result of the embodiment shows that the RT-RAA CRISPR / Cas 12b detection kit finally designed by the invention is high in specificity and does not have cross reaction with other viruses, the lowest visual detection limit reaches 4.77 * 10 < 1 > copies / mu L, the virus can be instantly detected when the RT-RAA CRISPR / Cas 12b detection kit is used for detecting the CDV, the labor and equipment cost is low, the period is short, and the detection time does not exceed 2 hours. The rapid detection technology can be popularized and applied to epidemiological investigation and epidemic situation monitoring of the canine distemper virus, is suitable for epidemic disease monitoring of wild animals, quarantine of zoos and on-site rapid detection of clinical samples of canines, and has good practical significance and wide market prospects.

The utility model provides a micro-fluidic chip for unmarked or fluorescently marked single cell sorting and a single cell sorting system, which do not adopt means for damaging a sorting target, such as high voltage, strong electric field, high-voltage sheath fluid and the like, and can be used for sorting the unmarked or fluorescently marked single cells on the premise of keeping activity. For unlabeled or labeled (such as fluorescence labeled) cells, bacteria, proteins, viruses, genes (fragments or whole segments), cell clusters and other microorganisms, or liquid drops, liquid segments, bubbles or blocky solids containing the biological particles, high-efficiency, accurate and reliable single-individual detection, control and sorting (especially including but not limited to single-cell sorting) are carried out.

An oligonucleotide, a conjugate, a composition and the use thereof. The oligonucleotide can inhibit the gene expression of a hepatitis B virus, and can efficiently inhibit the generation of HBsAg and total HBV DNAs, and therefore is expected to realize functional healing of hepatitis B.

The disclosure relates to block copolymer nanoparticles for therapeutic delivery of nucleotides, and methods therefor. More particularly, the invention relates to polymer nanoparticles, such as reversible addition-fragmentation chain transfer (RAFT) polymer compositions, for delivering miRNAs.

The present invention relates to a diagnostic kit for bovine viral diarrhea virus (BVDV), which enables rapid on-site identification and diagnosis of BVDV1 and BVDV2, which are genotypes of the BVD virus that are pathogens causing bovine diarrhea, within a short time of approximately 15 minutes.

The present disclosure provides methods for treating HBV infection using combination therapies, and related kits and compositions for use. The components of the combination therapies include an inhibitor of HBV gene expression or an agent that reduces HBV antigenic load, and an anti-HBV antibody.

The invention relates to the technical field of animal epidemic disease detection, in particular to a portable African swine fever detection method based on an ERA-CRISPR / Cas12a technology, space-time separation of ERA constant-temperature nucleic acid amplification and CRISPR / Cas12a detection is realized through a specially designed double-layer reaction tube, and detection can be completed within 30 minutes at 45 DEG C by matching with a 150g portable constant-temperature nucleic acid amplification analyzer. According to the method, an African swine fever virus B646L gene is used as a target, crRNA and Cas12a protein are used for recognizing a target sequence to activate a fluorescence signal, the detection sensitivity reaches 12.1 copies / mu L, and the method has no cross reaction on PRRSV, PRV and other common swine pathogens. The instrument supports Bluetooth connection with a mobile phone to display a fluorescence curve in real time, a result can be automatically judged through a threshold value or is observed by naked eyes through ultraviolet light, the problems of expensive equipment and complicated operation in the prior art are solved, and the instrument is suitable for on-site rapid detection.

The invention belongs to the technical field of virus detection, and discloses a digital PCR (polymerase chain reaction) kit for simultaneously detecting African swine fever virus, porcine reproductive and respiratory syndrome virus and swine fever virus, and the kit comprises primers and probes as shown in SEQ ID NO.1-SEQ ID NO.24. After a single system of an African swine fever virus-VP72 gene, an African swine fever virus-CD2V gene, an African swine fever virus-14L gene, a porcine reproductive and respiratory syndrome virus-RdRP gene, a porcine reproductive and respiratory syndrome virus-European strain, a porcine reproductive and respiratory syndrome virus-American strain, a swine fever virus-vaccine strain and a swine fever virus-wild strain is tested, a nine-fold digital PCR system containing an internal standard is established; through tests of indexes such as linearity, specificity, minimum detection limit, repeatability, comparison of a single digital PCR system and a multiple digital PCR system, the currently developed digital PCR multiple detection kit completely meets development requirements and is high in specificity and sensitivity, and the minimum detection limit of the digital PCR multiple detection kit can reach 10 Copies / mu L or below.

The invention discloses a virus gene sequence host prediction method and system, and belongs to the technical field of biological information analysis, and the method comprises the steps: dividing a virus gene sequence into ordered k-mer word sequences, and carrying out the vectorization of the k-mer word sequences through a pre-trained BERT language model, and obtaining an embedded vector; mapping the embedded vector of the high-dimensional space into a low-dimensional space with a fixed dimension by adopting an average pooling method, and reducing the embedded dimension to obtain a feature vector representing an original gene sequence; the feature vectors are input into a pre-trained classification network model to predict the likelihood that the sequence is infected with a particular host. According to the method, the complete virus gene sequence is directly used as input, so that information loss possibly caused by dependence on statistical characteristics is avoided, and the accuracy of characteristic extraction is remarkably improved.

The invention discloses a construction method of CiYMaV CRP mutant infectious clone. A CiYMaV infectious clone vector is prepared by inserting a CiYMaV complete sequence into the downstream of a 2 * 35S promoter of a vector pCASS4-RZ; the mutant is obtained by carrying out alanine site-directed mutation or deletion mutation on a CRP (C-reactive protein) region on a CiYMaV infectious cloning vector through overlap extension PCR (polymerase chain reaction), and the site-directed mutation or deletion mutation is obtained on the basis of CiYMaV strong pathogenicity infectious cloning pCiYMaV. According to the invention, a vector pCASS4-RZ is used as a vector skeleton to construct an infectious clone containing strong pathogenicity of CiYMaV, and a series of CRP mutant infectious clone which is coded by viruses and has different pathogenicity and pathogenic expressions are constructed at the same time; virus inoculation experiments show that the CRP protein affects virus accumulation and symptom formation, and a foundation is laid for research on pathogenic mechanisms, virus gene functions and low virulent line cross protection of citrus yellow mottle related viruses and subsequent Mandarivirus virus research.

The invention discloses an application of bortezomib in preparation of a medicine for resisting grouper iridovirus. Researches show that bortezomib has a remarkable inhibiting effect on grouper iridovirus, and is low in cytotoxicity and good in safety. The bortezomib can obviously reduce the fluorescence signal intensity of virus protein, inhibit the transcriptional level of main capsid protein MCP and envelope protein VP19 of the virus and reduce the copy number of DNA and mRNA of the virus; the bortezomib can effectively inhibit grouper iridovirus infection and effectively block synthesis of virus protein, so that efficient inhibition of grouper iridovirus infection is achieved, and along with prolonging of virus infection time, bortezomib can also remarkably inhibit virus gene transcription, protein synthesis and genome replication. Therefore, bortezomib not only can effectively prevent and treat grouper iridovirus infection, but also has the characteristics of high specificity, low toxicity, remarkable antiviral effect and the like, and also has important application value in prevention and control of iridovirus-related diseases in aquaculture industry.

The invention provides a rapid processing method for HPV (human papillomavirus) gene sequence data. The method relates to the technical field of data processing, and comprises the following steps: acquiring and integrating HPV sequence data, host transcriptome data and clinical association data to form a comprehensive input matrix; performing feature screening on the comprehensive input matrix by adopting a bee colony optimization algorithm based on reinforcement learning to obtain an optimal feature subset; according to the bee colony optimization algorithm, global search of the bee colony algorithm is combined with a dynamic decision-making mechanism of reinforcement learning, and a feature selection strategy is dynamically adjusted; and constructing an HPV subtype accurate typing and variation detection model based on multi-task deep learning, performing deep coding and weighted fusion on the optimal feature subset through an unstable attention mechanism, and outputting two key prediction results of subtype probability and mutation site probability. According to the method, the accuracy and biological interpretability of virus typing and variation detection are improved, and efficient extraction and multi-task accurate recognition of high-dimensional features are realized.

The invention discloses a primer for detecting and distinguishing genotypes of avian infectious bronchitis viruses and application, and belongs to the technical field of biological detection. The primer combination comprises a universal primer and a probe group I for detecting an IBV total group, a specific primer and a probe combination II for detecting a GI-19 genotype, and a specific primer and a probe combination III for detecting a GVI-1 genotype, and nucleotide sequences are respectively shown as SEQ ID NO.1-9; different fluorophores are respectively marked at 5'ends of each group of probes, and corresponding quenching groups are marked at 3 'ends of each group of probes. The primer combination provided by the invention has high specificity and high sensitivity, rapid detection of the IBV and accurate distinguishing of main epidemic genotypes (GVI-1 and GI-19) can be completed in a single reaction, complex sequencing and analysis steps in a traditional method are omitted, the detection efficiency is greatly improved, and the primer combination has a good application prospect. And a convenient tool is provided for farm epidemic situation monitoring and clinical diagnosis.

The invention discloses a fiveleaf virus GII.3 whole genome sequencing primer based on an amplicon sequencing technology and application thereof, and belongs to the technical field of virus gene sequencing. According to the whole genome sequencing primer and the sequencing method for the fiveleaf virus GII.3, interference of other microorganisms on sequencing can be avoided, the gene sequencing depth is high, the advantages of being wider in whole genome coverage, high in detection accuracy and high in specificity are achieved, the sequencing result can reflect mutation characteristics of main epidemic strains of the fiveleaf virus, and the sequencing result can be used for detecting the mutation characteristics of the main epidemic strains of the fiveleaf virus GII.3. And a powerful tool is provided for rapid diagnosis and full prevention of the fiveleaf akebia viruses.

The invention provides a Marburg virus detection standard substance as well as a preparation system and application thereof, and belongs to the technical field of biological detection. The armored RNA standard substance containing the Marburg virus NP gene segment, provided by the invention, contains the NP gene segment, can be used for nucleic acid detection of the Marburg virus taking the NP gene as a target spot, and is used for quality control of detection methods and reagents such as common PCR (Polymerase Chain Reaction), fluorescent quantitative PCR, digital PCR, LAMP (Loop-Mediated Isothermal Amplification) and the like. The armored RNA standard substance provided by the invention has the advantages of high purity, no plasmid DNA residue and good quality control performance. The armored RNA virus-like particle simulates the structure of a natural virus, and can participate in links such as nucleic acid extraction and amplification as an animal body or human body blood sample containing Marburg virus, so that the whole-course quality control of the whole detection process is realized, and the authenticity and reliability of the detection result are ensured.

The application discloses a method for creating a new strain of Carassius auratus gibelii with resistance to Cyprinid herpesvirus 2 (CyHV2) infection, and the method is characterized in that a gene editing technology is used to target knockout gsdf-a a gene and gsdf-b a gene, and an experimental animal infection model of a homozygous knockout strain of the gene obtained by the technology is established. gsdf Results of the experimental animal infection model show that gsdf the CyHV2 infection resistance of the CyHV2 gene knockout Carassius auratus gibelii is significantly enhanced, the histopathological damage of the Carassius auratus gibelii after being infected with the virus is reduced, the expression of a virus protein ORF47 in liver tissue of the Carassius auratus gibelii is reduced, and the transcription level of a virus gene orf46r is significantly reduced. The new strain of Carassius auratus gibelii created by the method can avoid exogenous gene pollution, and has the advantages that the CyHV2 infection resistance of the Carassius auratus gibelii is significantly enhanced.

The present invention provides dual-target site reverse transcription fluorescent PCR primers, probes, and a kit for detecting the novel coronavirus SARS-CoV-2. By performing nucleic acid sequence alignment on the entire genome of the novel coronavirus SARS-CoV-2, three specific nucleic acid sequences W1, W2, and W3 in the viral N gene are found, and the sequences are shown as SEQ ID NOs. 1-3, respectively. Multiple pairs of primers and probes are designed for the three target sites, respectively. It is found that the dual-target site reverse transcription fluorescent PCR using a primer-probe combination designed for the W1 and W2 targets (sequences shown as SEQ ID NOs. 4-6 and SEQ ID NOs. 7-9, respectively) can specifically and sensitively detect the novel coronavirus SARS-CoV-2, with a detection sensitivity of less than 10 copies. There is no non-specific amplification of 22 common clinical respiratory pathogens, which can effectively avoid false negative results caused by single target site mutations and has good specimen detection capabilities.

This invention provides a dengue / Zika virus genetically engineered vaccine and its application. The dengue / Zika virus vaccine comprises an open reading frame encoding envelope protein domain III (EDIII) and the non-structural protein NS1, and displays EDIII monomers in the delivery vector shell. Immunization with this vaccine primarily induces a type-specific antibody response, reducing the production of cross-antibodies and thus effectively avoiding or eliminating the risk of antibody-dependent enhancement of infection (ADE). This vaccine can be used to prevent dengue virus and Zika virus infection.

The present application relates to the technical field of knowledge graph, in particular to a multi-task hepatitis B drug screening method and system based on knowledge graph assistance, comprising the following steps: based on hepatitis B virus genotype sequence data recorded over time, patient drug use records and drug sensitivity. In the present application, multi-dimensional dynamic time series data such as virus genotype sequence data, patient drug use records and drug sensitivity are integrated, the interaction events between entities are marked by time stamp, the dynamic characteristics such as virus variation track and drug efficacy change are embedded in the graph node attributes, so that the knowledge representation can reflect the time dependence in the real scene. Based on biological pathway annotation information and protein interaction data, the hyperedge connection multi-entity set is defined, the limitation of traditional knowledge graph which only supports binary relationship is expanded, the drug combination and multi-target synergistic mechanism are explicitly modeled, and the misjudgment of combination effect caused by the simplification of interaction relationship is avoided.

Described herein is an immortalized mammalian cell configured for production of a bioproduct of interest, which cell has a safe harbor locus selected from an Adeno Associated Virus 1 (AAVS1) locus, a CCR5 locus, and a Rosa26 locus into which a Human Papillomavirus (HPV) E6 gene sequence and an HPV E7 gene sequence have been inserted. Also disclosed herein are methods for immortalizing a mammalian cell for production of a bioproduct of interest as well as methods of producing a bioproduct of interest.