285 results about "Viral gene" patented technology

The invention provides a virus propagation dynamic prediction method based on a multi-strain multi-region population model and related equipment. The method comprises the following steps: acquiring historical epidemic situation related data of a target area, wherein the historical epidemic situation related data comprises epidemic situation data, virus gene sequence data, population flow data and epidemic situation management policy data related to a target virus in a past time period of each area; arranging the population flow data into an asymmetric population flow matrix between different regions every day; sorting epidemic prevention grades corresponding to different epidemic situation management policies according to the epidemic situation management policy data; and constructing a multi-strain and multi-region population model based on epidemic situation data, virus gene sequence data, a population flow matrix and epidemic prevention grades corresponding to different epidemic situation management policies by taking StatPOMP as a model construction framework, and generating virus propagation dynamic prediction information based on the model. The prediction accuracy of the virus propagation trend can be improved.

The invention provides a structure, a sequence and a construction method of a plant virus gene editing vector and application of the plant virus gene editing vector in plant gene editing. The method is characterized in that a TRV2 genome of a tobacco embrittlement virus (TRV) is used for carrying and expressing a small Cas gene AsCas12f and gRNA of the small Cas gene AsCas12f. Preferably, the AsCas12f and the gRNA of the AsCas12f are subjected to tandem expression by utilizing the TRV2 to form an expression cassette structure of the AsCas12f-tRNA-gRNA, the expression cassette substitutes a part of 2b gene sequence of the TRV2 on a TRV2 genome to form a plant gene editing vector pTRV2-Cas12f-gRNA based on the TRV virus, and long-time stable expression and gene editing can be performed after wild type nicotiana benthamiana is inoculated. The invention also provides a method for carrying out gene editing in a wild type plant by utilizing the plant virus.

The invention discloses a live pig epidemic prevention and control flow regulation system and method based on big data, and relates to the technical field of animal health information, and the method comprises the steps: recognizing abnormal live pig signals and track intersection features of structured epidemic prevention feature data through an intelligent algorithm, comparing the virus gene difference degree, constructing a virus propagation network diagram, and carrying out the analysis of the virus propagation network diagram; outputting a high-risk propagation node map; constructing an aerosol dynamics three-dimensional space model by taking a super propagation hub node of the high-risk propagation node map as an original point and combining culture environment parameters, simulating a virus air diffusion path, and generating an environment propagation thermal distribution map; fusing the high-risk propagation node map and the environment propagation thermal distribution map, identifying a cross infection area through a multi-dimensional risk superposition model to perform risk dynamic grading, and outputting a grading early warning report; according to the invention, breakthrough improvement of the live pig epidemic prevention and control capability is realized, key nodes and potential risk areas of epidemic propagation can be accurately identified, and the pertinence of prevention and control measures is improved.

The invention provides a pseudovirus of a Nipah virus and application of the pseudovirus, and belongs to the technical field of genetic engineering. The invention provides a pseudovirus of a Nipah virus and application of the pseudovirus. The pseudovirus contains a nucleic acid fragment of a Nipah virus N gene of which the nucleotide sequence is shown as SEQ ID NO.1. The invention further provides a preparation method of the pseudovirus. The pseudovirus of the Nipah virus can be used as a ribonucleic acid standard substance of the pseudovirus of the Nipah virus, and is applied to the aspects of quantity transmission, calibration, instrument testing, evaluation and control of a testing method, quality verification, quality control, production process detection and the like of a laboratory related to Nipah virus detection. The pseudovirus of the Nipah virus, as the ribonucleic acid standard substance of the pseudovirus of the Nipah virus, has the following advantages of accurate fixed value, good uniformity, stable quantity value and traceability, and can promote the improvement of the detection capability and the technical level of a national Nipah detection laboratory.

The disclosure relates to block copolymer nanoparticles for therapeutic delivery of nucleic acids, and methods therefor. More particularly, the invention relates to polymer nanoparticles, such as reversible addition-fragmentation chain transfer (RAFT) polymer compositions, for delivering miRNAs.

The invention belongs to the field of canine distemper virus detection, and particularly relates to a canine distemper virus RT-RAA CRISPR / Cas 12b detection kit and a detection method thereof. According to the kit, isothermal amplification is performed by using an RT-RAA universal primer, and then detection is performed by using a CRISPR / Cas 12b detection system consisting of target sequence sgRNA, so that amplification of a target fragment of the N gene of the canine distemper virus and target detection visualization can be effectively realized. The result of the embodiment shows that the RT-RAA CRISPR / Cas 12b detection kit finally designed by the invention is high in specificity and does not have cross reaction with other viruses, the lowest visual detection limit reaches 4.77 * 10 < 1 > copies / mu L, the virus can be instantly detected when the RT-RAA CRISPR / Cas 12b detection kit is used for detecting the CDV, the labor and equipment cost is low, the period is short, and the detection time does not exceed 2 hours. The rapid detection technology can be popularized and applied to epidemiological investigation and epidemic situation monitoring of the canine distemper virus, is suitable for epidemic disease monitoring of wild animals, quarantine of zoos and on-site rapid detection of clinical samples of canines, and has good practical significance and wide market prospects.

An oligonucleotide, a conjugate, a composition and the use thereof. The oligonucleotide can inhibit the gene expression of a hepatitis B virus, and can efficiently inhibit the generation of HBsAg and total HBV DNAs, and therefore is expected to realize functional healing of hepatitis B.

The disclosure relates to block copolymer nanoparticles for therapeutic delivery of nucleotides, and methods therefor. More particularly, the invention relates to polymer nanoparticles, such as reversible addition-fragmentation chain transfer (RAFT) polymer compositions, for delivering miRNAs.

The present disclosure provides methods for treating HBV infection using combination therapies, and related kits and compositions for use. The components of the combination therapies include an inhibitor of HBV gene expression or an agent that reduces HBV antigenic load, and an anti-HBV antibody.

The invention relates to the technical field of animal epidemic disease detection, in particular to a portable African swine fever detection method based on an ERA-CRISPR / Cas12a technology, space-time separation of ERA constant-temperature nucleic acid amplification and CRISPR / Cas12a detection is realized through a specially designed double-layer reaction tube, and detection can be completed within 30 minutes at 45 DEG C by matching with a 150g portable constant-temperature nucleic acid amplification analyzer. According to the method, an African swine fever virus B646L gene is used as a target, crRNA and Cas12a protein are used for recognizing a target sequence to activate a fluorescence signal, the detection sensitivity reaches 12.1 copies / mu L, and the method has no cross reaction on PRRSV, PRV and other common swine pathogens. The instrument supports Bluetooth connection with a mobile phone to display a fluorescence curve in real time, a result can be automatically judged through a threshold value or is observed by naked eyes through ultraviolet light, the problems of expensive equipment and complicated operation in the prior art are solved, and the instrument is suitable for on-site rapid detection.

The invention discloses a virus gene sequence host prediction method and system, and belongs to the technical field of biological information analysis, and the method comprises the steps: dividing a virus gene sequence into ordered k-mer word sequences, and carrying out the vectorization of the k-mer word sequences through a pre-trained BERT language model, and obtaining an embedded vector; mapping the embedded vector of the high-dimensional space into a low-dimensional space with a fixed dimension by adopting an average pooling method, and reducing the embedded dimension to obtain a feature vector representing an original gene sequence; the feature vectors are input into a pre-trained classification network model to predict the likelihood that the sequence is infected with a particular host. According to the method, the complete virus gene sequence is directly used as input, so that information loss possibly caused by dependence on statistical characteristics is avoided, and the accuracy of characteristic extraction is remarkably improved.

The invention discloses an application of bortezomib in preparation of a medicine for resisting grouper iridovirus. Researches show that bortezomib has a remarkable inhibiting effect on grouper iridovirus, and is low in cytotoxicity and good in safety. The bortezomib can obviously reduce the fluorescence signal intensity of virus protein, inhibit the transcriptional level of main capsid protein MCP and envelope protein VP19 of the virus and reduce the copy number of DNA and mRNA of the virus; the bortezomib can effectively inhibit grouper iridovirus infection and effectively block synthesis of virus protein, so that efficient inhibition of grouper iridovirus infection is achieved, and along with prolonging of virus infection time, bortezomib can also remarkably inhibit virus gene transcription, protein synthesis and genome replication. Therefore, bortezomib not only can effectively prevent and treat grouper iridovirus infection, but also has the characteristics of high specificity, low toxicity, remarkable antiviral effect and the like, and also has important application value in prevention and control of iridovirus-related diseases in aquaculture industry.

The invention provides a rapid processing method for HPV (human papillomavirus) gene sequence data. The method relates to the technical field of data processing, and comprises the following steps: acquiring and integrating HPV sequence data, host transcriptome data and clinical association data to form a comprehensive input matrix; performing feature screening on the comprehensive input matrix by adopting a bee colony optimization algorithm based on reinforcement learning to obtain an optimal feature subset; according to the bee colony optimization algorithm, global search of the bee colony algorithm is combined with a dynamic decision-making mechanism of reinforcement learning, and a feature selection strategy is dynamically adjusted; and constructing an HPV subtype accurate typing and variation detection model based on multi-task deep learning, performing deep coding and weighted fusion on the optimal feature subset through an unstable attention mechanism, and outputting two key prediction results of subtype probability and mutation site probability. According to the method, the accuracy and biological interpretability of virus typing and variation detection are improved, and efficient extraction and multi-task accurate recognition of high-dimensional features are realized.

The invention discloses a primer for detecting and distinguishing genotypes of avian infectious bronchitis viruses and application, and belongs to the technical field of biological detection. The primer combination comprises a universal primer and a probe group I for detecting an IBV total group, a specific primer and a probe combination II for detecting a GI-19 genotype, and a specific primer and a probe combination III for detecting a GVI-1 genotype, and nucleotide sequences are respectively shown as SEQ ID NO.1-9; different fluorophores are respectively marked at 5'ends of each group of probes, and corresponding quenching groups are marked at 3 'ends of each group of probes. The primer combination provided by the invention has high specificity and high sensitivity, rapid detection of the IBV and accurate distinguishing of main epidemic genotypes (GVI-1 and GI-19) can be completed in a single reaction, complex sequencing and analysis steps in a traditional method are omitted, the detection efficiency is greatly improved, and the primer combination has a good application prospect. And a convenient tool is provided for farm epidemic situation monitoring and clinical diagnosis.

The application discloses a method for creating a new strain of Carassius auratus gibelii with resistance to Cyprinid herpesvirus 2 (CyHV2) infection, and the method is characterized in that a gene editing technology is used to target knockout gsdf-a a gene and gsdf-b a gene, and an experimental animal infection model of a homozygous knockout strain of the gene obtained by the technology is established. gsdf Results of the experimental animal infection model show that gsdf the CyHV2 infection resistance of the CyHV2 gene knockout Carassius auratus gibelii is significantly enhanced, the histopathological damage of the Carassius auratus gibelii after being infected with the virus is reduced, the expression of a virus protein ORF47 in liver tissue of the Carassius auratus gibelii is reduced, and the transcription level of a virus gene orf46r is significantly reduced. The new strain of Carassius auratus gibelii created by the method can avoid exogenous gene pollution, and has the advantages that the CyHV2 infection resistance of the Carassius auratus gibelii is significantly enhanced.

This invention provides a dengue / Zika virus genetically engineered vaccine and its application. The dengue / Zika virus vaccine comprises an open reading frame encoding envelope protein domain III (EDIII) and the non-structural protein NS1, and displays EDIII monomers in the delivery vector shell. Immunization with this vaccine primarily induces a type-specific antibody response, reducing the production of cross-antibodies and thus effectively avoiding or eliminating the risk of antibody-dependent enhancement of infection (ADE). This vaccine can be used to prevent dengue virus and Zika virus infection.

The present application relates to the technical field of knowledge graph, in particular to a multi-task hepatitis B drug screening method and system based on knowledge graph assistance, comprising the following steps: based on hepatitis B virus genotype sequence data recorded over time, patient drug use records and drug sensitivity. In the present application, multi-dimensional dynamic time series data such as virus genotype sequence data, patient drug use records and drug sensitivity are integrated, the interaction events between entities are marked by time stamp, the dynamic characteristics such as virus variation track and drug efficacy change are embedded in the graph node attributes, so that the knowledge representation can reflect the time dependence in the real scene. Based on biological pathway annotation information and protein interaction data, the hyperedge connection multi-entity set is defined, the limitation of traditional knowledge graph which only supports binary relationship is expanded, the drug combination and multi-target synergistic mechanism are explicitly modeled, and the misjudgment of combination effect caused by the simplification of interaction relationship is avoided.

The present application relates to the field of molecular biology, in particular to a primer probe composition, kit and detection method for 23 human papilloma virus genotyping detection, wherein the hydrolysis probe and molecular beacon probe are respectively designed in two different regions of HPV gene sequence E6 and L1, and the fluorescence collection point in the amplification stage is set in the high-temperature denaturation stage, so that the detection in the amplification stage and the melting curve stage is independent and does not interfere with each other, thereby realizing the combined use of real-time fluorescent PCR amplification detection technology and melting curve technology; the technology improves the single-well detection target flux of fluorescent PCR analysis technology, reduces the detection cost, and provides a more optimal detection method for HPV multiplex fluorescent PCR genotyping detection.

The application relates to the technical field of biological medicine, and particularly discloses a fish rhabdovirus recombinant adenovirus vector vaccine and a preparation method and application thereof. The recombinant adenovirus vaccine carrying the M gene of the SCRV virus is prepared by using a replication-defective recombinant adenovirus vector, and the preparation method comprises the following steps: mandarin fish rhabdovirus M gene cloning, construction of a Pshuttle-CMV-ECMV vector with a target gene SCRV-M, obtaining of a SCRV-M recombinant adenovirus plasmid, screening of the SCRV-M recombinant adenovirus, and preparation of the SCRV-M recombinant adenovirus vaccine. The SCRV-M recombinant adenovirus vaccine prepared by the application can be used for immunoprophylaxis of SCRV virus infection in the juvenile fish stage through a soaking mode, the SCRV-M recombinant adenovirus vaccine has passed an animal safety test, and has a wide application prospect in the prevention and control of fish rhabdovirus.

The invention discloses application of siRNA for targeted inhibition of rabies virus G gene and / or L gene in preparation of drugs for inhibiting rabies. SiRNA-G for targeted inhibition of a rabies virus G gene and siRNA-L for targeted inhibition of a rabies virus L gene are provided on the basis, the nucleotide sequence of a sense strand of the siRNA-G is as shown in SEQ ID NO: 1, and the nucleotide sequence of an antisense strand of the siRNA-G is as shown in SEQ ID NO: 2; the nucleotide sequence of the positive-sense strand of the siRNA-L is as shown in SEQ ID NO: 3, and the nucleotide sequence of the antisense strand of the siRNA-L is as shown in SEQ ID NO: 4. The siRNA-G and / or siRNA-L can effectively inhibit the expression of the G gene and the L gene of the rabies virus and reduce the proliferation of the rabies virus, and can be used as an effective small nucleic acid drug in the development process of anti-rabies virus drugs.

The present invention relates generally to compositions, systems and methods for modulating hepatitis B virus (HBV) by targeting gene repression. In particular, the present invention provides an epigenetically modified DNA targeting system, such as a CRISPR-Cas / guide RNA (gRNA) system, for transcriptional repression of hepatitis B virus genes to promote cell phenotypes that lead to reduced HBV infection. In some embodiments, the epigenetically modified DNA targeting systems of the present invention bind to or target a target site of at least one gene in the hepatitis B virus DNA sequence or a regulatory element thereof in a cell. In some aspects, the systems provided herein relate to transcriptional repression of one or more hepatitis B virus genes and / or regulatory elements thereof. In some aspects, the invention also provides methods and uses associated with the provided compositions, such as in repression of hepatitis B virus replication and expression associated with hepatitis B infection.

Herein is reported a method for producing a recombinant adeno-associated viral particle preparation (rAAVp), the method comprising the steps of culturing a mammalian cell comprising an expression cassette directed against: a non-adeno-associated viral gene interposed between two AAV inverted terminal repeats (ITRs), and thereby producing the rAAVp; an adeno-associated virus rep gene; an adeno-associated virus cap gene; an adeno-associated virus E1A gene; an adeno-associated virus E1B gene; an adeno-associated virus E2A gene; an adeno-associated virus E4orf6; and an adeno-associated virus VA RNA gene, wherein the culturing is carried out at a pH value in the range of pH 7.4 to pH 7.6 and including a terminal value. The yield of the rAAVp produced by the culture performed at a pH value in the range of pH 7.4 to pH 7.6 and including a terminal value is higher than the yield of the rAAVp produced by the culture performed at a pH value in the range of pH 7.0 to pH 7.2 and including a terminal value, and the yield of the rAAVp produced by the culture performed at a pH value in the range of pH 7.4 to pH 7.6 and including a terminal value is lower than the yield of the rAAVp produced by the culture performed at a pH value in the range of pH 7.0 to pH 7.2. And the rAAVp produced by the culture at a pH value in the range of pH 7.4 to pH 7.6 and including an endvalue has a higher percentage of intact particles than the rAAVp produced by the culture at a pH value in the range of pH 7.0 to pH 7.2 and including an endvalue.

The invention belongs to the field of veterinary virology, and discloses a bovine ephemeral fever virus strain and reverse genetic application thereof, and the reverse genetic steps are as follows: taking pCI plasmid as a skeleton to prepare plasmid for expressing a complete genome of bovine ephemeral fever virus; the method comprises the following steps: respectively preparing auxiliary plasmids for expressing an N protein gene, a P protein gene, an L protein gene and a G protein gene of the bovine ephemeral fever virus by taking pCAGGS plasmids as a skeleton, co-transfecting BHK-21 cells by using the five plasmids, and culturing the cells to obtain the rescued bovine ephemeral fever virus. The rescued bovine ephemeral fever virus has no obvious difference from the reproductive capacity of the parent virus, and the genome is still stable after 10 generations of continuous passage in BHK-21 cells. The invention provides methodological support for genetic modification of the bovine ephemeral fever virus, and has an application prospect.

The invention discloses a recombinant antigen protein, a recombinant vector, a recombinant host cell, a virus-like particle and application thereof and a vaccine, and relates to the technical field of antigen preparation, the recombinant antigen protein comprises an icosahedral porcine circovirus capsid protein and an A104R protein loaded on the surface of the icosahedral porcine circovirus capsid protein, the A104R protein comprises a protein coded by an African swine fever virus A104R gene. The recombinant antigen protein capable of simultaneously reacting with positive serum of the African swine fever virus and positive serum of the porcine circovirus is prepared, and the vaccine prepared by mixing the recombinant antigen protein with an adjuvant can effectively stimulate a pig body to generate specific antibodies of the African swine fever virus and the porcine circovirus; therefore, reference is provided for joint defense of the two viruses. The recombinant antigen protein provided by the invention has the characteristics of stable expression, high yield and high solubility.

The invention discloses application of mitoxantrone in preparation of an anti-rabies virus drug, reveals that clinical anti-tumor and immunosuppression drug mitoxantrone has remarkable anti-rabies virus activity for the first time, and opens up a new application field for mitoxantrone. The rigorous in-vitro and in-vivo tests prove that the mitoxantrone can inhibit virus replication in a dose-dependent manner, reduce virus gene and protein expression and remarkably improve the survival rate of infected mice in an animal model. The invention provides a new clinical application approach for mitoxantrone, provides a powerful candidate drug and a brand-new technical scheme for anti-rabies virus drugs, and has important practical significance and clinical application prospects for coping with rabies which is a major public health threat.

The invention provides a vector and a method for expressing astaxanthin by using nicotiana benthamiana leaves, which are characterized in that plant virus expression vectors of calendula astaxanthin synthesis related genes AdKC and AdKeto and plant virus gene silencing (RNAi) vectors of nicotiana benthamiana carotenoid cleavage dioxygenase 1 (CCD1) are respectively constructed; the agrobacterium tumefaciens of the three vectors are mixed and infiltrated into the boeng tobacco leaves to obtain the leaves for expressing the astaxanthin. According to the invention, the expression of the nicotiana benthamiana CCD1 gene is interfered while the AdKC and AdKeto genes are expressed in the nicotiana benthamiana leaves, so that the content of astaxanthin expressed in the nicotiana benthamiana leaves is increased.

The invention belongs to the technical field of virus and gene engineering, and particularly relates to an expression vector of a respiratory syncytial virus (RSV) ON1 genotype consensus sequence strain and an editing strain, a reverse genetic manipulation system and an application of the reverse genetic manipulation system of the respiratory syncytial virus ON1 genotype consensus sequence strain and the editing strain of the RSV ON1 genotype consensus sequence strain and the editing strain of the respiratory syncytial virus ON1 genotype consensus sequence strain. According to the invention, an RSV-ON1 genotype consensus sequence strain is firstly constructed and saved, on the basis, 72 nucleotides which are repeatedly inserted into a G gene of the consensus sequence strain are removed through a gene editing technology, and two strains with different virus G gene sequences are obtained. According to the invention, through construction of RSV infectious clone edited by RSV-ON1 genotype G gene and rescue of the two strains, a fast, simple and accurate RSV reverse genetic system is constructed, and the RSV reverse genetic system can be applied to research on RSV in-vitro virus replication mechanism and pathogenesis and neutralizing antibody immune escape. A etiological technical platform is provided for research, development and evaluation of RSV vaccines, antibodies, drugs and the like.

The invention discloses a porcine epidemic diarrhea virus G2c genotype strain as well as a separation method and application thereof. Aiming at the current epidemic PEDV variant strain, the genetic variation rule of the epidemic strain is clarified by carrying out virus separation, identification and pathogenicity analysis, and the method has important significance on epidemiology, vaccine development and the like of the PEDV. Specifically, an applicant successfully separates a PEDV strain capable of realizing stable passage propagation in Vero-CCL81 cells from intestinal contents of diarrhea piglets collected from a pig farm of Jiangpermanent Province in Hunan, the PEDV strain is identified and determined as a porcine epidemic diarrhea virus G2c subtype, the PEDV strain is named PEDV CH / HNJY / 2025, the PEDV strain is preserved in China General Microbiological Culture Collection Center on December 11, 2025, and the preservation number of the PEDV strain is CGMCC NO. The preservation number is CGMCC (China General Microbiological Culture Collection Center)

The invention provides an application of cudrania tricuspidata polysaccharide in preparation of a medicine for treating or preventing HCMV infection, the cudrania tricuspidata polysaccharide ZGs-1 shows a remarkable inhibition effect on expression of immediate early protein IE1 / 2 and early protein p52 and copy numbers of virus genes UL123, UL44 and UL32 in a plurality of key links of HCMV infection, and has definite antiviral activity. Due to the natural source characteristic of the cudrania tricuspidata polysaccharide ZGs-1, the cudrania tricuspidata polysaccharide ZGs-1 is high in safety and low in cytotoxicity, and the cudrania tricuspidata raw material is easy to obtain, so that a candidate drug or functional component with remarkable potential is provided for preventing and treating the HCMV, and the cudrania tricuspidata polysaccharide ZGs-1 has a good development prospect and application value.

A nonviral gene therapy delivers DNA that allows cells to produce proteins such as their own growth factors in culture media. Lipid nanoparticles provide an effective delivery mechanism into a target cell. By encapsulating plasmid DNA encoding the growth factors with inducible promoters into lipid nanoparticles (LNPs), target cells create their own growth factors reducing a need for expensive growth factors from external sources. DNA expression lasts for several days reducing the amount of reagent needed. The plasmid DNA is more stable than mRNA and proteins, therefore reducing logistical challenges.