522 results about "Viral gene" patented technology

The invention provides a recombinant Marek's disease virus co-expressing a Newcastle disease virus F gene and an infectious bursal disease virus supervirulent strain VP2 gene and application thereof, and belongs to the field of genetic engineering vaccines. According to the invention, by using a recombinant cloning technology, an expression framework containing a Newcastle disease virus gene VII type strain F gene and an infectious bursal disease virus super virulent strain VP2 gene is inserted into a spacer region between Marek's disease virus genome UL45 and UL46 genes; according to the method, a recombinant cosmid is constructed and obtained, wherein a Newcastle disease virus F gene and an infectious bursal disease virus super virulent strain VP2 gene expression framework are inserted between Marek's disease virus genome UL45 and UL46 genes, and a recombinant virus C20101 strain co-expressing the Newcastle disease virus F gene and the infectious bursal disease virus super virulent strain VP2 gene is rescued and obtained by the recombinant cosmid. The vaccine prepared from the recombinant Marek's disease virus strain provided by the invention can prevent Newcastle disease, chicken infectious bursal disease and Marek's disease at the same time, and has a good market application prospect.

The invention relates to the technical field of knowledge maps, in particular to a multi-task hepatitis B drug screening method and system based on knowledge map assistance, and the method comprises the following steps: based on hepatitis B virus genotype sequence data recorded along with time, patient drug use records and drug sensitivity. According to the method, virus genotype sequence data, patient drug use records, drug sensitivity and other multi-dimensional dynamic time sequence data are integrated, interaction events between entities are marked through timestamps, and virus variation tracks, drug curative effect changes and other dynamic characteristics are embedded into atlas node attributes; and the knowledge representation can reflect the time dependence in a real scene. A hyperedge connection multi-entity set is defined based on biological pathway annotation information and protein interaction data, the limitation that only a binary relation is supported in a traditional knowledge graph is expanded, a drug combination and multi-target synergistic effect mechanism is explicitly modeled, and misjudgment of the combination effect caused by simplification of the interaction relation is avoided.

The invention provides a primer probe composition and a kit for detecting a Databean virus and application, and relates to the technical field of biological detection, the primer probe composition comprises a primer probe group aiming at a Databean virus S gene and a primer probe group aiming at a Databean virus L gene, the Databean virus L and S genes are used as amplification target genes for design, and the kit is used for detecting the Databean virus. The primer probe group aiming at the S gene of the Databu virus and the primer probe group aiming at the L gene of the Databu virus are used as the primer probe composition, so that multi-gene target amplification is realized, and the positive detection rate reaches 100% and is higher than the positive detection rate of single-gene amplification; the lowest detection limit is up to 200 copies / mL; the quantification limit reaches 500 copies / mL, and the quantification linear range is 5.0 * 10 < 2 >-1.0 * 10 < 8 > copies / mL. The technical problem that in the prior art, a high-sensitivity and high-accuracy multiplex qPCR detection method for the nucleic acid of the copyda virus is lacked is solved.

The invention discloses a pneumovirus gene data multi-view clustering integration method and device and electronic equipment. The method comprises the following steps: constructing a width self-encoder with a width learning network and a global subspace structure; training a width auto-encoder, determining model parameters of the width auto-encoder, inputting multi-view pneumonia gene data into the trained width auto-encoder, and outputting a compressed feature subspace; clustering the compressed feature subspaces to obtain a plurality of basic partitions as integration members of an integration pool; the basic partition is converted into a fuzzy partition matrix, an undirected bipartite graph is constructed based on the integration pool, and connection weights between nodes of the undirected bipartite graph correspond to values in the fuzzy partition matrix; and cutting the undirected bipartite graph into several disjoint sub-graphs, and obtaining a clustering integration result of multi-view pneumonia gene data according to the sample nodes of each sub-graph. According to the method, parameter quantity and computing resources are remarkably reduced, and multi-view clustering analysis of pneumonia gene data is efficiently completed.

The invention relates to a Nipah virus N protein specific monoclonal antibody as well as a preparation method and application thereof. The nipah virus specific monoclonal antibody is prepared by immunizing nipah virus N protein, the amino acid sequence of the nipah virus N protein is as shown in SEQ ID NO.2, the nipah virus N protein is obtained by transcription of a nipah virus N gene, and the nucleotide sequence of the nipah virus N gene is as shown in SEQ ID NO.1. The nipah virus specific monoclonal antibody has the advantages that the nipah virus specific monoclonal antibody is prepared by immunizing the nipah virus N protein; the monoclonal antibody with the nipah virus N protein specificity can be specifically combined with the nipah virus N protein and is high in titer and good in affinity, meanwhile, a detection kit prepared based on the antibody can effectively carry out nipah virus detection, and technical support can be provided for epidemiological monitoring and diagnosis of nipah virus infection.

The present disclosure belongs to the field of biotechnology, and specifically relates to a method for efficiently infecting human natural killer (NK) cells and other immune cells with a pseudovirus. Specifically, a viral transfection system provided in the present disclosure has an envelope plasmid with a protein having an X—Y—Z structure. The X is an extracellular (ex) structure of a gibbon ape leukemia virus (GALV) envelope glycoprotein, the Y is a transmembrane (TM) structure of the GALV envelope glycoprotein, and the Z is an intracellular segment portion of a murine virus gene.

The invention provides a virus propagation dynamic prediction method based on a multi-strain multi-region population model and related equipment. The method comprises the following steps: acquiring historical epidemic situation related data of a target area, wherein the historical epidemic situation related data comprises epidemic situation data, virus gene sequence data, population flow data and epidemic situation management policy data related to a target virus in a past time period of each area; arranging the population flow data into an asymmetric population flow matrix between different regions every day; sorting epidemic prevention grades corresponding to different epidemic situation management policies according to the epidemic situation management policy data; and constructing a multi-strain and multi-region population model based on epidemic situation data, virus gene sequence data, a population flow matrix and epidemic prevention grades corresponding to different epidemic situation management policies by taking StatPOMP as a model construction framework, and generating virus propagation dynamic prediction information based on the model. The prediction accuracy of the virus propagation trend can be improved.

The invention provides a construction method and application of a recombinant herpesvirus turkey for co-expressing an NDV (Newcastle Disease Virus) F gene and an H9 subtype AIV (Avian Influenza Virus) HA gene, and belongs to the field of genetic engineering vaccines. The recombinant clone technology is utilized, an expression framework containing a Newcastle disease virus gene VII type strain F gene and an H9 subtype avian influenza virus HA gene is inserted between 95322-95323 nucleotides or between 112071-112088 nucleotides of a turkey herpesvirus FC126 strain, and the recombinant clone with the F gene and HA gene expression framework inserted into a turkey herpesvirus genome is constructed and obtained. Recombinant turkey herpesvirus vaccine strains H20901 and H20902 for co-expressing the Newcastle disease virus F gene and the H9 subtype avian influenza virus HA gene are rescued and obtained by the recombinant turkey herpesvirus vaccine strain H20901 and H20902. The vaccine prepared from the recombinant turkey herpesvirus provided by the invention can provide dual protection for the Newcastle disease virus and the avian influenza virus, and has a good market application prospect.

The invention belongs to the technical field of virus detection, and discloses a digital PCR (Polymerase Chain Reaction) kit for simultaneously detecting African horse pestivirus, West Nile virus and equine influenza H3N8, and the kit comprises primers and probes as shown in SEQ ID NO.1-SEQ ID NO.21. The invention also discloses a kit for simultaneously detecting African horse pestivirus, West Nile virus and equine influenza H3N8. According to the invention, after a single system of an African horse pestivirus VP7 gene, an African horse pestivirus-NS2 gene, a west nile virus-NS5 gene, a west nile virus-NS2a gene, an equine influenza H3N8-HA gene and an equine influenza H3N8-NA gene is tested, a seven-fold digital PCR (Polymerase Chain Reaction) system containing an internal standard is established; through tests of indexes such as linearity, specificity, minimum detection limit, repeatability, comparison of a single digital PCR system and a multiple digital PCR system, the currently developed digital PCR multiple detection kit completely meets development requirements and is high in specificity and sensitivity, and the minimum detection limit of the digital PCR multiple detection kit can reach 10 Copies / mu L or below.

The invention provides a structure, a sequence and a construction method of a plant virus gene editing vector and application of the plant virus gene editing vector in plant gene editing. The method is characterized in that a TRV2 genome of a tobacco embrittlement virus (TRV) is used for carrying and expressing a small Cas gene AsCas12f and gRNA of the small Cas gene AsCas12f. Preferably, the AsCas12f and the gRNA of the AsCas12f are subjected to tandem expression by utilizing the TRV2 to form an expression cassette structure of the AsCas12f-tRNA-gRNA, the expression cassette substitutes a part of 2b gene sequence of the TRV2 on a TRV2 genome to form a plant gene editing vector pTRV2-Cas12f-gRNA based on the TRV virus, and long-time stable expression and gene editing can be performed after wild type nicotiana benthamiana is inoculated. The invention also provides a method for carrying out gene editing in a wild type plant by utilizing the plant virus.

The invention discloses a live pig epidemic prevention and control flow regulation system and method based on big data, and relates to the technical field of animal health information, and the method comprises the steps: recognizing abnormal live pig signals and track intersection features of structured epidemic prevention feature data through an intelligent algorithm, comparing the virus gene difference degree, constructing a virus propagation network diagram, and carrying out the analysis of the virus propagation network diagram; outputting a high-risk propagation node map; constructing an aerosol dynamics three-dimensional space model by taking a super propagation hub node of the high-risk propagation node map as an original point and combining culture environment parameters, simulating a virus air diffusion path, and generating an environment propagation thermal distribution map; fusing the high-risk propagation node map and the environment propagation thermal distribution map, identifying a cross infection area through a multi-dimensional risk superposition model to perform risk dynamic grading, and outputting a grading early warning report; according to the invention, breakthrough improvement of the live pig epidemic prevention and control capability is realized, key nodes and potential risk areas of epidemic propagation can be accurately identified, and the pertinence of prevention and control measures is improved.

The present invention relates to a viral gene therapy vector for removing hepatitis B viruses, a preparation method therefor, and the use thereof, aiming to solve the problem in which existing viral gene therapy vectors can only inhibit the replication of hepatitis B viruses, but cannot remove virus cccDNA. The vector comprises a promoter, an intron, an enhancer, a nuclear localization signal (NLS) functional domain encoding gene of PGLYRP2 protein, an HBV DNA binding domain encoding gene of the PGLYRP2 protein, a secretory signal peptide functional domain encoding gene of the PGLYRP2 protein, an HBV nucleocapsid-binding functional domain encoding gene of the PGLYRP2 protein, and an IRES fragment. The viral gene therapy vector can remarkably promote the removal of HBVs in the liver of mice and is used for treatment of hepatitis B virus infection.

The present invention relates to methods of producing microbubble drug conjugates, viral gene therapy microbubble drug conjugates, and disease-targeting microbubbles, for clinical and preclinical ultrasound-mediated therapeutic and diagnostic applications. It includes methods to produce viral vector gene therapy microbubble drug conjugates with antibody linkers conjugated to lipid shelled microbubbles that both bind to and neutralize viral vectors such that the viral gene therapy can transduce and effect permanent genetic changes only after ultrasound is used to disassociate the viral gene therapy from microbubbles at diseased regions of the body.

Provided herein are epigenetic-modifying DNA-targeting systems, such as CRISPR-Cas / guide RNA (gRNA) systems, for the transcriptional repression of Hepatitis B viral (HBV) genes to promote a cellular phenotype that leads to the reduction of HBV infection. In some embodiments, the epigenetic-modifying DNA-targeting systems bind to or target a target site of at least one gene or regulatory element thereof in a Hepatitis B viral DNA sequence in cell. In some aspects, the provided systems relate to the transcriptional repression of one or more Hepatitis B viral gene and / or regulatory element thereof. In some aspects, also provided herein are methods and uses related to the provided compositions, for example in repressing Hepatitis B viral replication and expression in connection with Hepatitis B infections.

The invention provides a pseudovirus of a Nipah virus and application of the pseudovirus, and belongs to the technical field of genetic engineering. The invention provides a pseudovirus of a Nipah virus and application of the pseudovirus. The pseudovirus contains a nucleic acid fragment of a Nipah virus N gene of which the nucleotide sequence is shown as SEQ ID NO.1. The invention further provides a preparation method of the pseudovirus. The pseudovirus of the Nipah virus can be used as a ribonucleic acid standard substance of the pseudovirus of the Nipah virus, and is applied to the aspects of quantity transmission, calibration, instrument testing, evaluation and control of a testing method, quality verification, quality control, production process detection and the like of a laboratory related to Nipah virus detection. The pseudovirus of the Nipah virus, as the ribonucleic acid standard substance of the pseudovirus of the Nipah virus, has the following advantages of accurate fixed value, good uniformity, stable quantity value and traceability, and can promote the improvement of the detection capability and the technical level of a national Nipah detection laboratory.

The disclosure relates to block copolymer nanoparticles for therapeutic delivery of nucleic acids, and methods therefor. More particularly, the invention relates to polymer nanoparticles, such as reversible addition-fragmentation chain transfer (RAFT) polymer compositions, for delivering miRNAs.

The invention discloses a fiveleaf virus GI.2 whole genome sequencing primer based on an amplicon sequencing technology and application thereof, and belongs to the technical field of virus gene sequencing. The primer group composed of 18 pairs of primers is preferably obtained according to an amplicon sequencing technology, the primer group can effectively improve the primer amplicon coverage degree, it is guaranteed that the primer amplicon coverage degree is 1.5-1.8 times that of a virus sequence, the amplification time is shortened, the amplification difficulty is reduced, the primer amplicon coverage degree and the whole genome capture efficiency are improved, and the primer amplification efficiency is improved. And the method has important significance on epidemic situation traceability and propagation trend of the fiveleaf virus GI.2.

The invention discloses a peste des petits ruminants virus specific monoclonal antibody and application thereof.The monoclonal antibody is obtained by screening peste des petits ruminants virus N protein, the amino acid sequence of the peste des petits ruminants virus N protein is shown as SEQ ID NO: 1, the peste des petits ruminants virus N protein is obtained by transcription of a peste des petits ruminants virus N gene, and the peste des petits ruminants virus N gene is shown as SEQ ID NO: 2. The nucleotide sequence of the codon-optimized peste des petits ruminants virus N gene is shown as SEQ ID NO: 2. The monoclonal antibody provided by the invention can be specifically combined with the peste des petits ruminants virus N protein, the titer is high, the affinity is good, meanwhile, the detection kit prepared based on the antibody pair can effectively carry out peste des petits ruminants virus detection, and a technical support can be provided for epidemiological monitoring and diagnosis of peste des petits ruminants virus infection.

The invention discloses a polyclonal antibody of a grouper iridovirus SGIV VP101 gene and application of the polyclonal antibody. The polyclonal antibody is prepared from an SGIVVP101 gene, and the nucleotide sequence of the VP101 gene of an optimized codon is as shown in SEQ ID NO. 1. The polyclonal antibody of the SGIV VP101 gene recombinant expression protein prepared by the invention has good specificity, not only can specifically recognize a prokaryotic expression product of the SGIV VP101, but also can be used for quantitative and positioning analysis of the SGIV VP101 in vivo and in vitro. The successful preparation of the polyclonal antibody of the recombinant expression protein of the SGIV VP101 gene lays a foundation for the establishment of an SGIV immunological detection method, the quantitative and positioning analysis of the protein of the VP101 gene and the analysis of gene functions.

The invention relates to a heat-resistant H1N1 subtype influenza virus mutant vaccine strain and application thereof. The invention belongs to the technical field of virus gene engineering, and particularly discloses a heat-resistant H1N1 subtype influenza virus mutant vaccine strain and application thereof. According to the H1N1 subtype influenza virus mutant vaccine strain and the preparation method thereof, the HA protein head region, which is a key region influencing the virus thermal stability, is mutated, the virus thermal stability can be remarkably improved only by mutating three amino acid sites, meanwhile, the virus still has good thermal stability after being inactivated, the H1N1 subtype influenza virus mutant vaccine strain prepared by HA protein point mutation has the preservation number of CCTCC NO: V202471, and the H1N1 subtype influenza virus mutant vaccine strain can be used for preparing the H1N1 subtype influenza virus mutant vaccine strain. And immune animals can be induced to generate high-level HI antibodies.

We queried DNA from saliva or plasma of 93 HNSCC patients, searching for somatic mutations or human papillomavirus genes, collectively referred to as tumor DNA. When both plasma and saliva were tested, tumor DNA was detected in 96% (95% CI, 84% to 99%) of 47 patients. The fractions of patients with detectable tumor DNA in early- and late-stage disease were 100% (n=10) and 95% (n=37), respectively. Saliva is preferentially enriched for tumor DNA from the oral cavity, whereas plasma is preferentially enriched for tumor DNA from the other sites. Tumor DNA in the saliva and plasma is a valuable biomarker for detection of HNSCC.

The invention belongs to the field of canine distemper virus detection, and particularly relates to a canine distemper virus RT-RAA CRISPR / Cas 12b detection kit and a detection method thereof. According to the kit, isothermal amplification is performed by using an RT-RAA universal primer, and then detection is performed by using a CRISPR / Cas 12b detection system consisting of target sequence sgRNA, so that amplification of a target fragment of the N gene of the canine distemper virus and target detection visualization can be effectively realized. The result of the embodiment shows that the RT-RAA CRISPR / Cas 12b detection kit finally designed by the invention is high in specificity and does not have cross reaction with other viruses, the lowest visual detection limit reaches 4.77 * 10 < 1 > copies / mu L, the virus can be instantly detected when the RT-RAA CRISPR / Cas 12b detection kit is used for detecting the CDV, the labor and equipment cost is low, the period is short, and the detection time does not exceed 2 hours. The rapid detection technology can be popularized and applied to epidemiological investigation and epidemic situation monitoring of the canine distemper virus, is suitable for epidemic disease monitoring of wild animals, quarantine of zoos and on-site rapid detection of clinical samples of canines, and has good practical significance and wide market prospects.

The utility model provides a micro-fluidic chip for unmarked or fluorescently marked single cell sorting and a single cell sorting system, which do not adopt means for damaging a sorting target, such as high voltage, strong electric field, high-voltage sheath fluid and the like, and can be used for sorting the unmarked or fluorescently marked single cells on the premise of keeping activity. For unlabeled or labeled (such as fluorescence labeled) cells, bacteria, proteins, viruses, genes (fragments or whole segments), cell clusters and other microorganisms, or liquid drops, liquid segments, bubbles or blocky solids containing the biological particles, high-efficiency, accurate and reliable single-individual detection, control and sorting (especially including but not limited to single-cell sorting) are carried out.

The present invention provides an improved method and system for identifying nucleic acid sequences within a set of sequences obtained by a sequencer, constructing database according to the identification of nucleic acid sequences, identifying different genus, species, sub-species, serotypes, variety of microorganism, virus, genes, or nucleic acid sequences of interest, for its use in the field of molecular biology applied to diagnosis, in hospitals, schools, industry or any place wherein this method and system is required to identify nucleic acid sequences, obtained by a sequencer. Specifically, the present invention provides an improved method and system that allow sequences to be differentiated from data obtained by nucleic acid sequencing.

An oligonucleotide, a conjugate, a composition and the use thereof. The oligonucleotide can inhibit the gene expression of a hepatitis B virus, and can efficiently inhibit the generation of HBsAg and total HBV DNAs, and therefore is expected to realize functional healing of hepatitis B.

The disclosure relates to block copolymer nanoparticles for therapeutic delivery of nucleotides, and methods therefor. More particularly, the invention relates to polymer nanoparticles, such as reversible addition-fragmentation chain transfer (RAFT) polymer compositions, for delivering miRNAs.

The present invention relates to a diagnostic kit for bovine viral diarrhea virus (BVDV), which enables rapid on-site identification and diagnosis of BVDV1 and BVDV2, which are genotypes of the BVD virus that are pathogens causing bovine diarrhea, within a short time of approximately 15 minutes.

The invention relates to the technical field of biology, and particularly discloses a recombinant vector, a recombinant baculovirus and a preparation method and application of the recombinant vector and the recombinant baculovirus. According to the method for preparing the recombinant vector, pFastBac Dual is adopted as a vector to prepare a recombinant plasmid vector inserted into a VP2 gene of an avian bursal disease virus classic strain, and the recombinant plasmid vector is named as pDual-V1; the method comprises the following steps: preparing a recombinant plasmid vector into which VP2 genes of a classical strain and a variant strain of the avian bursal disease virus are inserted at the same time, and naming the recombinant plasmid vector as pDual-V1-V2; the method comprises the following steps: preparing a recombinant plasmid vector inserted into a group I type 4 fowl adenovirus Fiber-2 gene, and naming the recombinant plasmid vector as pDual-F2; the method comprises the following steps: preparing a recombinant plasmid vector into which a Fiber-2 gene and an avian reovirus sigma C gene sequence are inserted at the same time, wherein the recombinant plasmid vector is named as pDual-F2-sigma C; carrying out double enzyme digestion on the pDual-V1-V2, recovering small fragments, carrying out double enzyme digestion on the pDual-F2-sigma C, recovering large fragments, and then carrying out mixed enzyme ligation. The recombinant baculovirus can express four antigens at the same time, and effective preparation of a multi-combined vaccine is achieved.

The present disclosure provides methods for treating HBV infection using combination therapies, and related kits and compositions for use. The components of the combination therapies include an inhibitor of HBV gene expression or an agent that reduces HBV antigenic load, and an anti-HBV antibody.

The invention relates to the technical field of biological medicines, and particularly discloses a recombinant adenovirus vaccine for viral nervous necrosis of fish as well as a preparation method and application of the recombinant adenovirus vaccine. The method for preparing the viral nervous necrosis disease recombinant adenovirus vaccine by utilizing the replication-defective recombinant adenovirus vector comprises the following specific steps: cloning nervous necrosis virus CP gene, constructing a Pshuttle-CMV-ECMV vector with a target gene, obtaining recombinant adenovirus plasmids, screening to obtain recombinant adenovirus and preparing the recombinant adenovirus vaccine. The prepared recombinant adenovirus vaccine for the viral nervous necrosis of the fish can be used for immunizing and preventing NNV virus infection in a juvenile fish stage in a soaking manner, the stability of the vaccine is good, and animal safety tests prove that the vaccine is safe, effective and convenient and simple in clinical use, and has a relatively good market application prospect.