85results about How to "Keep natural activity" patented technology

The invention relates to a method for preparing a health-care food (therapy) product (nutrient juice wine) by use of extracts from cordyceps militaris and cocoon, aiming at extracting multiple nutrient substances with natural activity from cordyceps militaris and cocoon wastes to produce different types of dietary supplements for fully improving the utilization rate of cordyceps militaris and cocoon. The method is characterized in that the nutrient components can be extracted from the cordyceps militaris and the cocoon through freezing and thawing, combined enzymolysis, and a water extraction and alcohol precipitation step-by-step extraction method, and the health-care food (therapy) product has three formulas (in part by weight) according to different functions (requirements): A. nutritive extract of cordyceps militaris and cocoon 85% and assistant 15%; B. nutritive extract of cordyceps militaris and cocoon 95% and assistant 5%; and C. nutritive extract of cordyceps militaris and cocoon 98% and assistant 2%, wherein the assistant can be starch, beta dextrin and the like, can be combined with one of A, B and C formulas to produce the health-care food (therapy) product with different efficacies through the processes such as sterilization, enzymolysis, precipitation, residue filtering, saccharification and fermentation.

The invention relates to a method for preparing nutrient (liquid) beverages and granules of healthcare food (therapy) products by using extractives of cordyceps militaris and cocoons and aims to meet market requirements by producing nutrient healthcare products with different types by using various nutrient substances extracted from waste cocoons of the cordyceps militaris. The method is characterized by comprising the following steps of: firstly extracting the nutrient substances from the cordyceps militaris and the cocoons by adopting freeze thawing, combined enzymolysis and a water-extraction alcohol-precipitation sequential extraction method, and producing nutrient liquors and granules with different functions according to different functions (requirements) and different proportions. According to the method for preparing the nutrient (liquid) beverages and the granules of the healthcare food (therapy) products by using the extractives of the cordyceps militaris and the cocoons, the synergistic effects among nutritional ingredients are adequately exerted, and the use ratios of the cordyceps militaris and the cocoons are improved.

The invention discloses a method for preparing health food capsules by using cordyceps militaris and cocoon extracts, and aims to extract various nutrient substances with natural activities from the cordyceps militaris and cocoon to produce different types of nutrient health products, so that the utilization rate of the cordyceps militaris and cocoon is fully improved, and the market demand is met. The method is characterized by comprising the steps of extracting the nutrient substances from the cordyceps militaris and cocoon by performing freeze thawing, combined enzymolysis and aqueous alcohol precipitation sub-step extracting methods; adding a proper quantity of auxiliary materials into the nutrient substances to fully and evenly stir and sieve to prepare the particles; filling the particles into the capsules; wiping off residual powder adhered to the capsule shells; waxing; polishing; and sterilizing the capsules in a radiation mode after the capsules are packaged so as to obtain the finished product.

The invention discloses rhodiola rosea fermentation protoplasm cosmetic and a preparation method thereof. Efficient ingredients of the rhodiola rosea fermentation protoplasm cosmetic are supernate obtained by mixing powder of rhodiola rosea root, water and zymophyte to obtain an initial system, fermenting the initial system to obtain zymotic fluid and sterilizing and centrifuging the zymotic fluid. The zymophyte is saccharomycete. The rhodiola rosea root is subjected to whole plant fermentation by adopting the saccharomycete, all functional ingredients and activity of the plant are kept, and loss of active ingredients caused by an extracting method is avoided. Any organic reagents are not added during extraction, fermentation temperature and fermentation pH are mild, structure of plant active ingredients is not damaged, natural activity of the plant is kept, and chemical components of essence and the like are not added in the fermentation protoplasm so as to ensure the safety of products on the human body. The rhodiola rosea fermentation protoplasm cosmetic is rich in salidroside, arbutin and the like having activities of restraining tyrosinase activity and restraining melanin synthesis so as to have a relatively strong whitening function on skin and has a synergistic effect with the components in the fermentation filtrate simultaneously.

The invention relates to porcine circovirus PCV2 ORF2 gene optimized by using escherichia coli expression codon and a preparation method of PCV2 ORF2 capsid protein virus-like particles derived from the escherichia coli.

The invention discloses ampelopsis grossedentata selenium-enriched functional drink and a preparation method thereof and relates to natural health functional drink added with no preservative. The ampelopsis grossedentata selenium-enriched functional drink is prepared from the following components in percentage by weight: 10-80% of ampelopsis grossedentata extracting solution, 1-20% of cordyceps militaris extracting solution, 0.01-0.05% of vitamin C, 0.2-0.8% of beta-cyclodextrin, 0.01-0.05% of flavoured enzyme, (1-5)*10<-4>% of plant organic selenium and the balance of water. The prepared drink has the health effects of resisting to oxidation, protecting liver, helping digestion and improving immunity and can meet market diversification demand of drink.

The invention discloses a rapid and high sensitive molecular biosensor and a method of single-molecule detection of DNA or protein using the molecular biosensor. The molecular biosensor disclosed herein comprises: a) at least a single-walled carbon nanotube transistor device, wherein, the single-walled carbon nanotube transistor device comprises a grid electrode, a source electrode, a drain electrode and a conducting channel, the conducting channel is a single-walled carbon nanotube which is cut into two sections to form two single-walled carbon nanotubes with a gap of 1-10 nm; and b) an aptamer, wherein, the terminals of the aptamer are modified by terminal amino groups, and the aptamer is connected with the two ends of the carbon nanotubes forming the gap through an amide bond. The method is a reliable and practical method with general applicability. According to the method, nano / molecular electronics and the biosystem are combined, and the direct and real-time detection of the activities of DNA or protein with single-minded selectivity and ultrahigh sensitivity is realized by using functionalized mono-molecular devices and electric signal measurement.

The present invention discloses a method for extracting ganoderma spore oil by using supercritical CO2 extraction process. Said method includes the following steps: (1), using wall-broken ganoderma spore powder as raw material, mixing the wall-broken ganoderma spore powder with a certain quantity of granular filling material according to mixing ratio of 10:1-1:10; (2), placing the mixed raw material into an extraction still, introducing CO2 and extracting for 2-5 h at 10-35 MPa and 35-55 deg.C; (3), controlling first-level separation still pressure to make it be 8-10 MPa, making separation at 30-50 deg.C, after the extraction is completed, discharging light-yellow ganoderma spore oil from outlet of separation still; and (4), controlling second-level separation still pressure to make its be 4-6 MPa, making separation at 30-50 deg.C, after the extraction is completed, discharging small quantity of water.

The present invention discloses an active collagen lyophilized powder and a preparation method thereof. A rat tail collagen, polyols, mycose and citric acid are compounded, so that the stability of the rat tail collagen in a freeze-drying process may be jointly improved, a prepared lyophilized product may be stored without a preservative for a long time and is good in redissolution property, capable of keeping the natural bioactivity of collagen and remarkable in capability of promoting the synthesis of collagen in the skin.

The invention discloses a recombinant protein for diagnosing bovine tuberculosis, which is obtained by a method comprising the following steps: extracting the genome DNA of mycobacterium tuberculosisvar. bovis as a template of a PCR reaction to amplify out CFP10 and ESAT6 genetic fragments; using a gene splicing technique to amplify out a target gene CFP10-ESAT6 fusion gene; cloning and transforming the target gene to obtain a recombinant fungus, and inducing and expressing the recombinant fungus according to a conventional method, and purifying an expression product to obtain the recombinantprotein. The recombinant protein and a diagnostic reagent provided by the invention are used for diagnosing the bovine tuberculosis to obtain the advantages of low cost, high speed, high sensitivityand specificity.

The invention relates to a production technology of mineral water. The production technology comprises the following steps: (1), selecting mountain spring water of a main peak of Dabie Mountains, filtering the mountain spring water with coconut shell activated carbon, and filtering the filtered mountain spring water once again with gravel and activated carbon; (2), performing reverse osmosis filtration on the mountain spring water treated in the step (1), so that colloid microorganisms in the mountain spring water are removed; (3), performing iron removal with a manganese sand filter, wherein the water current velocity during the iron removal is 18-20m<3> / h; (4), performing sterilization with ozone, and then performing disinfection with ultraviolet rays; (5), inletting clean carbon dioxide gas into the disinfected mountain spring water; (6), inletting clean mixed gas into the mountain spring water into which the clean carbon dioxide gas is inlet, so that the oxygen content in the mountain spring water of 25 DEG C achieves 65mg / L; (7), performing sterile filling on the mountain spring water. The method provided by the invention is simple; the mineral water contains few chemical additives, is good in mouth feel, is high in oxygen content, and has good health-care value.

The invention discloses a method for efficiently preparing recombinant active scorpion insect neurotoxin LqhIT2. The method mainly comprises a step (1) of optimizing and synthesizing LqhIT2 genes with C- ends provided with 6*His labels according to amino acid sequences of mature LqhIT2 toxins and characteristics of a pichia pastoris expression system and constructing pichia pastoris secretory expression vectors; a step (2) of converting recombinant vectors into a pichia pastoris host, and through screening, obtaining yeast transformants in high-level secretory expression; a step (3) of expressing recombinant scorpion insect neurotoxin LqhIT2 proteins in the pichia pastoris host, and quickly obtaining the recombinant scorpion insect neurotoxin LqhIT2 proteins with the purity over 98% through a Ni2+ affinity chromatography method, wherein the purified recombinant proteins have very strong poisonous activity on insect cells. The method is modified based on conventional expression of insect neurotoxin LqhIT2 through prokaryotic host escherichia coli, the method of using eukaryotic host pichia pastoris to express the insect neurotoxin LqhIT2 is explored, and the yeast transformants in high-level secretory expression are screened.

The invention relates to a high-activity cellulose incision enzyme artificially synthesized gene. The gene is shown as a nucleotide sequence of SEQ ID NO.1 in a sequence table; or the gene is as shownin the sequence of a biological function protein with 90 or above homology with the nucleotide sequence and same coding. According to the high-activity cellulose incision enzyme artificially synthesized gene, such as the nucleotide sequence of SEQ ID NO.1 in the sequence table, a pichia pastoris constitutive expression plasmid pGAPZalpha can be used as a carrier, and high-level recombinant secretory expression of a target protein in pichia pastoris host bacteria X33 can be realized. Meanwhile, screened high-level expression converters can realize high-level serection of a recombinant proteinby using high-density fermentation of an inorganic salt, and novel high-activity recombinant cellulose incision enzyme protein sterling can be obtained through simple nickel affinity chromatography purification. The recombinant protein has the high ability of hydrolyzing cellulose substances and generating glucose, and has important application value in aspects such as biological energy sources, fodder and food industries.

The invention provides a preparation method of asiatic pennywort herb fermented raw stock, wherein the preparation method comprises the steps: mixing asiatic pennywort herb powder, water and a zymocyte solution to obtain an initial system, fermenting to obtain a fermentation solution, sterilizing the obtained fermentation solution, centrifuging to obtain supernatant, and thus obtaining the asiaticpennywort herb fermented raw stock. According to the adopted fermentation method, no organic reagent is added in the process of extracting effective components of asiatic pennywort herb, the fermentation temperature and the fermentation pH are mild, the structures of the plant active components are not damaged, and the natural activity of plants is maintained.

The invention discloses a process for preparing donkey placenta freeze-dried powder by using a liquid nitrogen freezing and grinding method. The process comprises the steps of pre-freezing fresh donkey placenta at -10 to 0 DEG C so as to form frozen donkey placenta, then slicing the donkey placenta, and firstly freezing the donkey placenta slices in liquid nitrogen and then putting into a freezingmixed ball mill for grinding into homogenate; after that, placing the homogenate in a cold trap of a freeze dryer, controlling the temperature of the cold trap and carrying out freeze-drying to obtain freeze-dried donkey placenta; then, ultra-finely grinding the freeze-dried donkey placenta to obtain donkey placenta powder with uniform plasmid, packaging, sealing, and then carrying out low-dose transient irradiation by using Co60-electron beams to obtain the donkey placenta freeze-dried powder. After the process is adopted, the donkey placenta tissues can be rapidly and effectively ground within a very short time by using the liquid nitrogen freezing and grinding method, and biomacromolecules of the donkey placenta tissues are fully released in a solution, so that the natural activity ofthe biomacromolecules is better maintained, and the unique color of the donkey placenta is maintained; the donkey placenta freeze-dried powder containing rich nutritional ingredients can be obtained,and the production efficiency and the product yield are increased.

The invention relates to a process for producing mineral water. The process comprises the following steps: (1) selecting spring water of a main peak of Dabie Mountain, filtering the spring water withcocoanut shell activated charcoal, and filtering the water again by using sandstone and activated charcoal; (2) carrying out reverse osmosis filtering on the water treated in the step (1) to remove colloidal microbes from the water; (3) carrying out iron removal by using a manganese sand filtering device, wherein a water velocity is 18m<3> / h to 20m<3> / h during iron removal; (4) carrying out sterilization by using ozone, and then, carrying out disinfection by ultraviolet rays; (5) introducing clean carbon dioxide into the water; (6) introducing clean mixed gas into the water so as to enable theoxygen content of water of 25 DEG C to reach 65mg / L; (7) carrying out sterile filling on the water. The process provided by the invention is simple, the amount of chemical additives is small, and themineral water is good in taste and high in oxygen content and has very good health-care value.

The invention provides a membrane separation and purification technical technology for preparing bitter gourd polypeptide protein, a bitter gourd polypeptide protein extract and an application of thebitter gourd polypeptide protein extract, and relates to the technical fields of deep processing of bitter gourds and polypeptide extraction and purification. The membrane separation and purificationtechnical technology comprises the following steps of cleaning bitter gourds as raw materials, performing pulping, performing crushing, performing leaching, performing centrifuging, and removing residues so as to obtain centrifugate; filtering the centrifugate through a micro filtration membrane system so as to obtain micro filtration membrane penetration fluid; performing separation and purification on the penetration fluid on two coiling type ultra filtration membrane purification systems of different molecular weight so as to obtain ultra filtration membrane trapped fluid; concentrating thetrapped fluid through a high-pressure reverse osmosis membrane so as to obtain concentrate of which the solid matter content is greater than or equal to 30%; and drying the concentrate. The new technical technology effectively guarantees the natural activity of the bitter gourd polypeptide protein and the content of the bitter gourd polypeptide protein being higher than 25%; and the prepared extract contains a great amount of bitter gourd polypeptide having the function of adjusting and controlling the metabolism of blood sugar and having specific molecular weight and sequence and has good treatment effects on diabetes, and the bitter gourd polypeptide protein extract can be made into foods for special dietary uses, health-care foods or drugs for treating the diabetes.

The invention discloses a multifunctional health-care beverage. The beverage is prepared from the following raw materials in parts by weight: 10-40 parts of kudzuvine roots, 2-5 parts of raisin tree seed extract, 2-5 parts of perilla extract, 10-15 parts of hawthorn fruits, 17-23 parts of Chinese wolfberry fruits, 12-17 parts of stevia and 1-3 parts of cassia seeds. The preparation method of the beverage comprises the following steps: (1) material preparation; (2) raw material pretreatment; (3) mixing; (4) enzymolysis; (5) alcohol fermentation; (6) vinegar fermentation; (7) separating; (8) spraying; (9) ageing; (10) filtration; (11) blending; (12) homogenization; (13) fine filtration; (14) sterilization; and (15) filling. The multifunctional health-care beverage has the effects of relieving hangover, protecting liver, nourishing stomach, reducing blood sugar, reducing blood fat and the like, the natural activity of the preparation raw materials is kept in the preparation process, and the production method is simple, short in cycle and low in labor intensity.

The invention discloses a method for producing barley green, which belongs to the technical field of methods for producing the barley green. In order to solve the problems, the adopted proposal is that the method for producing the barley green comprises the following steps sequentially: cleaning young barley grasses; crushing the barley grasses to break plant cell walls; and filter-pressing, sterilizing, concentrating and drying the barley grasses to obtain the finished product, wherein the step of sterilization adopts a sleeve type sterilization method which is performed at a working temperature of between 90 and 125 DEG C, the time for the sterilization of between 120 to 180 seconds, and the temperature of materials kept to be between 40 and 70 DEG C; and the temperature control method in the step of the sterilization is an industrial automatic temperature control method for measuring and controlling the temperature. The method can be widely applied to production fields of various natural barley greens.

The present invention relates to an artificial synthesis gene for coding cellulose excision enzymes. The gene at least contains a DNA piece with one of the following nucleotide sequences: 1) a nucleotide sequence of SEQ ID NO.1 in a sequence table; and 2) a nucleotide sequence which has more than or equal to 90% of homology with the nucleotide sequence shown in SEQ ID NO. 1 and codes a protein with a same biological function. According to the gene sequence, a recombinant vector is further constructed to transform yeasts, so that secretory expression of a recombinant cellulose excision enzyme under induction of methanol can be realized, the active recombinant cellulose excision enzyme protein with a purity higher than 95% can be obtained through nickel affinity purification, and the activeprotein has capacity of hydrolyzing cellulose and generating glucose.

The invention discloses a preparation method for a spider neurotoxin TX4(6-1) recombinant protein. The preparation method comprises the following steps: (1) according to the amino acid sequence of TX4(6-1) mature toxin and the feature of a pichia pastoris expression system, optimizing and synthesizing gene of TX4(61-) of which the C-end is provided with a 6XHis label and constructing a pichia pastoris secretory expression carrier; (2) converting a recombinant carrier to a pichia pastoris host, screening to obtain a yeast converter of high-level secretory expression; and (3) expressing a recombinant spider insect neurotoxin TX4(6-1) protein in the yeast host, to obtain the recombinant spider insect neurotoxin TX4(6-1) protein of which the purity is more than 95% rapidly through a nickel affinity chromatography method, wherein the protein has the remarkable toxicity to silkworms. According to the preparation method, a large number of the stable recombinant spider insect neurotoxin TX4(6-1) proteins can be rapidly obtained. The preparation method has important value for the insect resistant field, specifically for cultivating insect resistant plant varieties, and the preparation method has important significance for improving the yield of crops.

The invention discloses a preparation method of a scorpion neurotoxin Bj alpha IT gene and a recombinant protein thereof. The preparation method comprises the following steps that: (1) According to the amino acid sequence of the Bj alpha IT mature toxin and the characteristics of Pichia pastoris expression systems, Bj alpha IT genes with 6*His tags at C- ends are optimally synthesized and Pichia pastoris secretion expression vectors are constructed; (2) the recombinant vectors are transferred into Pichia pastoris hosts, yeast transformants with high level secretion expression are obtained, and recombinant scorpion insect Bj alpha IT proteins with a purity of over 95% are obtained by nickel affinity chromatography. A method for expressing insect neurotoxin Bj alpha IT genes with eukaryotic host Pichia pastoris is disclosed. The recombinant scorpion insect Bj alpha IT protein with high purity is quickly obtained by the nickel affinity chromatography, and the protein performs high bioactivity at both cellular and animal levels. The protein is of great value in the field of insect resistance, especially for breeding varieties of insect resistant plants, and has important significance for improving the yield of crops.

The invention provides a three flower fermentation original solution cosmetic product. The three flower fermentation original solution cosmetic product is prepared from a zymophyte solution with a lactobacillus plantarum concentration ranging from 10<6> to 10<8>CFU / ml, peach flower powder, Flores Chamomillae powder, rose flower powder, glucose, and water. The invention also discloses a preparationmethod of the three flower fermentation original solution cosmetic product. The preparation method comprises following steps: peach flower powder, Flores Chamomillae powder, rose flower powder, water, and zymophyte are mixed so as to obtain an initial fermentation system, the obtained fermentation solution is subjected to disinfection and centrifugation so as to obtain a supernatant, wherein thesupernatant is the three flower fermentation original solution cosmetic product. According to the preparation method, lactic acid bacteria is adopted for full plant fermentation of the three kinds offlowers, so that all plant functional components and the activities are maintained, active component loss caused by extraction method is avoided, and plant natural activity is maintained. The three flower fermentation original solution cosmetic product is rich in components, such as kaempferol, trifolin, vitamins, amino acids, rose polyphenols, and quercitrin, with excellent antioxidation activityand inhibition activity on tyrosinase.

The invention belongs to the technical field of biology. The invention relates to a polypeptide containing two dominant epitopes of canine brain natriuretic peptide (BNP). The immune effect is enhanced by coupling the polypeptide with KLH protein immune mice. The invention also provides a recombinant protein. The recombinant protein comprises two dominant epitopes of canine BNP, and in order to improve the yield of the recombinant protein in a prokaryotic expression system, escherichia coli preferred codons are adopted to convert the amino acid sequence of the recombinant protein into a corresponding nucleotide sequence, the nucleotide sequence is chemically synthesized, and a recombinant protein expression vector is constructed. The invention further relates to a phage library established by coupling polypeptide with KLH protein immune mice, a corresponding canine BNP single-chain antibody scfv sequence is obtained through panning and screening, the obtained scfv sequence is constructed into a complete mouse IgG1 antibody sequence expression vector, monoclonal antibodies are expressed through transient HEK293F cells, the monoclonal antibodies are purified, and the optimal monoclonal antibody pairing combination is determined through an immunofluorescence orthogonal experiment.

The invention relates to recombinant pectinase, a gene thereof, a recombinant vector, a preparation method and application. The gene at least comprises DNA fragment of one of a nucleotide sequence ofSEQ ID NO.1 in a sequence table, a nucleotide sequence having higher than 90% homology with the nucleotide sequence shown as the SEQ ID NO.1 and coding same biological functional protein and a nucleotide sequence hybridized with the nucleotide sequence shown as the SEQ ID NO.1 and coding same biological functional protein. The recombinant vector is further built according to the gene sequence, yeast is transformed, in this way, constitutive secretory expression of the recombinant pectinase in a high-density fermentation condition can be realized; active protein higher than 95% in purity and with high protein concentration can be obtained through simple nickel affinity purification; the active protein has high pectin hydrolyzing activity at low temperature, and a novel and efficient enzymeproduct can be provided for increasing juice yield of fruit juice.

The invention discloses a method for extracting nutritional ingredients such as free amino acids from waste cordyceps militaris cocoons, aiming at extracting various nutritional substances keeping natural activity from the waste cordyceps militaris cocoons, thus increasing the utilization rate of cordyceps militaris and the cocoons, and supplying for production for different types of nutritional healthcare products. The method is characterized by adopting a sequential extraction process of freeze-thawing, combined enzymatic hydrolysis, and water extraction and alcohol precipitation, wherein generally, two of the following enzymes are used in a matching manner, and the hydrolysis temperatures of the enzymes are as follows respectively: the hydrolysis temperature of papain is 45+ / -2 DEG C; the hydrolysis temperatures of lysozyme, pectinase, amylase, pepsase and trypsin are 37+ / -2 DEG C; the hydrolysis temperatures of bromelain, glucoamylase, monascus and cellulose are 35+ / -2 DEG C; and the dosages of the enzymes are as follows respectively: in case of enzymatic hydrolysis for 1 kg of cordyceps militaris cocoon powder, 1 g of lysozyme for food, 2 g of amylase, 2 g of papain, 2 g of pepsase, 2 g of trypsin, 3 g of bromelain, 3 g of glucoamylase, 3 g of monascus, 3 g of cellulose and 4 g of pectinase are added.

The invention provides four-substance fermented water, and a preparation method and application thereof. The preparation method includes the steps that mixed powder of prepared rehmannia roots, angelica sinensis roots, szechuan lovage rhizome and radix paeoniae alba, water and zymophyte liquid are mixed to obtain an initial system, then fermentation is conducted to obtain fermented liquid, and thefermented liquid is sterilized and centrifuged to obtain a supernatant, namely the four-substance fermented water. The prepared rehmannia roots, the angelica sinensis roots, the szechuan lovage rhizome and the radix paeoniae alba are fermented by adopting two lactic acid bacteria, all functional components and activities of the prepared rehmannia roots, the angelica sinensis roots, the szechuan lovage rhizome and the radix paeoniae alba are reserved, and loss of active components caused by an extraction method is avoided.

The invention relates to sterile bovine colostrum whey with natural immunocompetence, and a preparation method and an application thereof. The preparation method comprises the steps of centrifuging bovine colostrum, removing fat to obtain defatted bovine colostrum, settling casein in the defatted bovine colostrum by an isoelectric point method or a chymosin method, obtaining whey liquid, or dissolving bovine colostrum whey powder to obtain the whey liquid, pre-filtering with a hollow fiber membrane or a ceramic membrane, removing the residual fat, casein and most thalli in the whey, conducting filtration sterilization with a polytetrafluoroethylene membrane filter, filling under a sterile condition, and obtaining the sterile bovine colostrum whey. The sterile bovine colostrum whey is degermed by a polytetrafluoroethylene degerming membrane based on pre-filtering, the handling capacity of feed liquid is increased, the service life of the membrane is prolonged, the membrane cost is saved, and the sterile bovine colostrum whey is suitable for industrial production. The content of immunoglobulin in the obtained whey is high; sterile requirements can be met; and the whey can directly serve as an immunoglobulin-rich whey product, and can also be added to beverages such as milk and fruit juice to be made into functional beverages.

The invention discloses a method for preparing gelsolin from Dendrorhynchus zhejiangensis, which is implemented by carrying out RNA (ribose nucleic acid) extraction, cDNA (complementary deoxyribonucleic acid) synthesis, enzyme digestion, connection and transformation, recombinant plasmid transformation, recombinant protein inducible expression, and recombinant protein separation and purification on the Dendrorhynchus zhejiangensis body wall, wherein after the cDNA is synthesized, a proper double-digestion primer is used for carrying out PCR (polymerase chain reaction) amplification, thereby obtaining a PCR product with the target segment; primers and a proper expression vector are subjected to double-digestion and connected, and are transformed onto a proper host; and the separation and purification are carried out under non-metamorphic conditions by an improved operation method using a Ni-NTAHis.BindResin kit, thereby saving the complex renaturation step, and being beneficial to keeping the natural activity of the recombinant protein and obtaining recombinant gelsolin with higher purity. The recombinant gelsolin prepared by the method disclosed by the invention can exist in a soluble form, and has an obvious inhibiting action on tumor cells; the concentration of the recombinant gelsolin is about 85%; and the method disclosed by the invention is scientific and reasonable, and is simple to implement and easy to popularize.

The invention discloses a water shield beverage. The water shield beverage is prepared from components in parts by weight as follows: 50-90 parts of purified water, 23-56 parts of original water shield juice, 4-7 parts of monocrystal rock sugar, 0.04-0.02 parts of sodium hexametaphosphate and 0.1-0.6 parts of potassium sorbate / EDTA (ethylene diamine tetraacetic acid)-2Na, wherein a preparation method of the original water shield juice comprises steps as follows: fresh water shields are collected, rinsed with clean water, sorted and cut into uniform particles, the particles are subjected to instantaneous sterilization at the high temperature of 100-110 DEG C, sterilized juice is subjected to heat preservation for 5-10 min at the temperature of 75-85 DEG C, and the original water shield juice is obtained. The produced water shield beverage is all natural and additive-free, maintains natural activity of the water shields to the greatest extent, is rich in nutrients such as collagen, active polysaccharides, cellulose, flavone, vitamins, mineral substances and the like, and has higher pharmaceutical value and nutritive value. Besides, the water shield beverage contains water shield particles, tastes better and can be better accepted by mass consumers.