85results about How to "Keep natural activity" patented technology

The invention relates to a method for preparing nutrient (liquid) beverages and granules of healthcare food (therapy) products by using extractives of cordyceps militaris and cocoons and aims to meet market requirements by producing nutrient healthcare products with different types by using various nutrient substances extracted from waste cocoons of the cordyceps militaris. The method is characterized by comprising the following steps of: firstly extracting the nutrient substances from the cordyceps militaris and the cocoons by adopting freeze thawing, combined enzymolysis and a water-extraction alcohol-precipitation sequential extraction method, and producing nutrient liquors and granules with different functions according to different functions (requirements) and different proportions. According to the method for preparing the nutrient (liquid) beverages and the granules of the healthcare food (therapy) products by using the extractives of the cordyceps militaris and the cocoons, the synergistic effects among nutritional ingredients are adequately exerted, and the use ratios of the cordyceps militaris and the cocoons are improved.

The invention discloses a method for preparing health food capsules by using cordyceps militaris and cocoon extracts, and aims to extract various nutrient substances with natural activities from the cordyceps militaris and cocoon to produce different types of nutrient health products, so that the utilization rate of the cordyceps militaris and cocoon is fully improved, and the market demand is met. The method is characterized by comprising the steps of extracting the nutrient substances from the cordyceps militaris and cocoon by performing freeze thawing, combined enzymolysis and aqueous alcohol precipitation sub-step extracting methods; adding a proper quantity of auxiliary materials into the nutrient substances to fully and evenly stir and sieve to prepare the particles; filling the particles into the capsules; wiping off residual powder adhered to the capsule shells; waxing; polishing; and sterilizing the capsules in a radiation mode after the capsules are packaged so as to obtain the finished product.

The invention discloses rhodiola rosea fermentation protoplasm cosmetic and a preparation method thereof. Efficient ingredients of the rhodiola rosea fermentation protoplasm cosmetic are supernate obtained by mixing powder of rhodiola rosea root, water and zymophyte to obtain an initial system, fermenting the initial system to obtain zymotic fluid and sterilizing and centrifuging the zymotic fluid. The zymophyte is saccharomycete. The rhodiola rosea root is subjected to whole plant fermentation by adopting the saccharomycete, all functional ingredients and activity of the plant are kept, and loss of active ingredients caused by an extracting method is avoided. Any organic reagents are not added during extraction, fermentation temperature and fermentation pH are mild, structure of plant active ingredients is not damaged, natural activity of the plant is kept, and chemical components of essence and the like are not added in the fermentation protoplasm so as to ensure the safety of products on the human body. The rhodiola rosea fermentation protoplasm cosmetic is rich in salidroside, arbutin and the like having activities of restraining tyrosinase activity and restraining melanin synthesis so as to have a relatively strong whitening function on skin and has a synergistic effect with the components in the fermentation filtrate simultaneously.

The invention discloses ampelopsis grossedentata selenium-enriched functional drink and a preparation method thereof and relates to natural health functional drink added with no preservative. The ampelopsis grossedentata selenium-enriched functional drink is prepared from the following components in percentage by weight: 10-80% of ampelopsis grossedentata extracting solution, 1-20% of cordyceps militaris extracting solution, 0.01-0.05% of vitamin C, 0.2-0.8% of beta-cyclodextrin, 0.01-0.05% of flavoured enzyme, (1-5)*10<-4>% of plant organic selenium and the balance of water. The prepared drink has the health effects of resisting to oxidation, protecting liver, helping digestion and improving immunity and can meet market diversification demand of drink.

The invention discloses a rapid and high sensitive molecular biosensor and a method of single-molecule detection of DNA or protein using the molecular biosensor. The molecular biosensor disclosed herein comprises: a) at least a single-walled carbon nanotube transistor device, wherein, the single-walled carbon nanotube transistor device comprises a grid electrode, a source electrode, a drain electrode and a conducting channel, the conducting channel is a single-walled carbon nanotube which is cut into two sections to form two single-walled carbon nanotubes with a gap of 1-10 nm; and b) an aptamer, wherein, the terminals of the aptamer are modified by terminal amino groups, and the aptamer is connected with the two ends of the carbon nanotubes forming the gap through an amide bond. The method is a reliable and practical method with general applicability. According to the method, nano/molecular electronics and the biosystem are combined, and the direct and real-time detection of the activities of DNA or protein with single-minded selectivity and ultrahigh sensitivity is realized by using functionalized mono-molecular devices and electric signal measurement.

The invention discloses a recombinant protein for diagnosing bovine tuberculosis, which is obtained by a method comprising the following steps: extracting the genome DNA of mycobacterium tuberculosisvar. bovis as a template of a PCR reaction to amplify out CFP10 and ESAT6 genetic fragments; using a gene splicing technique to amplify out a target gene CFP10-ESAT6 fusion gene; cloning and transforming the target gene to obtain a recombinant fungus, and inducing and expressing the recombinant fungus according to a conventional method, and purifying an expression product to obtain the recombinantprotein. The recombinant protein and a diagnostic reagent provided by the invention are used for diagnosing the bovine tuberculosis to obtain the advantages of low cost, high speed, high sensitivityand specificity.

The invention discloses a method for efficiently preparing recombinant active scorpion insect neurotoxin LqhIT2. The method mainly comprises a step (1) of optimizing and synthesizing LqhIT2 genes with C- ends provided with 6*His labels according to amino acid sequences of mature LqhIT2 toxins and characteristics of a pichia pastoris expression system and constructing pichia pastoris secretory expression vectors; a step (2) of converting recombinant vectors into a pichia pastoris host, and through screening, obtaining yeast transformants in high-level secretory expression; a step (3) of expressing recombinant scorpion insect neurotoxin LqhIT2 proteins in the pichia pastoris host, and quickly obtaining the recombinant scorpion insect neurotoxin LqhIT2 proteins with the purity over 98% through a Ni2+ affinity chromatography method, wherein the purified recombinant proteins have very strong poisonous activity on insect cells. The method is modified based on conventional expression of insect neurotoxin LqhIT2 through prokaryotic host escherichia coli, the method of using eukaryotic host pichia pastoris to express the insect neurotoxin LqhIT2 is explored, and the yeast transformants in high-level secretory expression are screened.

The invention provides a preparation method of asiatic pennywort herb fermented raw stock, wherein the preparation method comprises the steps: mixing asiatic pennywort herb powder, water and a zymocyte solution to obtain an initial system, fermenting to obtain a fermentation solution, sterilizing the obtained fermentation solution, centrifuging to obtain supernatant, and thus obtaining the asiaticpennywort herb fermented raw stock. According to the adopted fermentation method, no organic reagent is added in the process of extracting effective components of asiatic pennywort herb, the fermentation temperature and the fermentation pH are mild, the structures of the plant active components are not damaged, and the natural activity of plants is maintained.

The invention discloses a process for preparing donkey placenta freeze-dried powder by using a liquid nitrogen freezing and grinding method. The process comprises the steps of pre-freezing fresh donkey placenta at -10 to 0 DEG C so as to form frozen donkey placenta, then slicing the donkey placenta, and firstly freezing the donkey placenta slices in liquid nitrogen and then putting into a freezingmixed ball mill for grinding into homogenate; after that, placing the homogenate in a cold trap of a freeze dryer, controlling the temperature of the cold trap and carrying out freeze-drying to obtain freeze-dried donkey placenta; then, ultra-finely grinding the freeze-dried donkey placenta to obtain donkey placenta powder with uniform plasmid, packaging, sealing, and then carrying out low-dose transient irradiation by using Co60-electron beams to obtain the donkey placenta freeze-dried powder. After the process is adopted, the donkey placenta tissues can be rapidly and effectively ground within a very short time by using the liquid nitrogen freezing and grinding method, and biomacromolecules of the donkey placenta tissues are fully released in a solution, so that the natural activity ofthe biomacromolecules is better maintained, and the unique color of the donkey placenta is maintained; the donkey placenta freeze-dried powder containing rich nutritional ingredients can be obtained,and the production efficiency and the product yield are increased.

The invention provides a membrane separation and purification technical technology for preparing bitter gourd polypeptide protein, a bitter gourd polypeptide protein extract and an application of thebitter gourd polypeptide protein extract, and relates to the technical fields of deep processing of bitter gourds and polypeptide extraction and purification. The membrane separation and purificationtechnical technology comprises the following steps of cleaning bitter gourds as raw materials, performing pulping, performing crushing, performing leaching, performing centrifuging, and removing residues so as to obtain centrifugate; filtering the centrifugate through a micro filtration membrane system so as to obtain micro filtration membrane penetration fluid; performing separation and purification on the penetration fluid on two coiling type ultra filtration membrane purification systems of different molecular weight so as to obtain ultra filtration membrane trapped fluid; concentrating thetrapped fluid through a high-pressure reverse osmosis membrane so as to obtain concentrate of which the solid matter content is greater than or equal to 30%; and drying the concentrate. The new technical technology effectively guarantees the natural activity of the bitter gourd polypeptide protein and the content of the bitter gourd polypeptide protein being higher than 25%; and the prepared extract contains a great amount of bitter gourd polypeptide having the function of adjusting and controlling the metabolism of blood sugar and having specific molecular weight and sequence and has good treatment effects on diabetes, and the bitter gourd polypeptide protein extract can be made into foods for special dietary uses, health-care foods or drugs for treating the diabetes.

The invention discloses a method for producing barley green, which belongs to the technical field of methods for producing the barley green. In order to solve the problems, the adopted proposal is that the method for producing the barley green comprises the following steps sequentially: cleaning young barley grasses; crushing the barley grasses to break plant cell walls; and filter-pressing, sterilizing, concentrating and drying the barley grasses to obtain the finished product, wherein the step of sterilization adopts a sleeve type sterilization method which is performed at a working temperature of between 90 and 125 DEG C, the time for the sterilization of between 120 to 180 seconds, and the temperature of materials kept to be between 40 and 70 DEG C; and the temperature control method in the step of the sterilization is an industrial automatic temperature control method for measuring and controlling the temperature. The method can be widely applied to production fields of various natural barley greens.

The invention discloses a preparation method of a scorpion neurotoxin Bj alpha IT gene and a recombinant protein thereof. The preparation method comprises the following steps that: (1) According to the amino acid sequence of the Bj alpha IT mature toxin and the characteristics of Pichia pastoris expression systems, Bj alpha IT genes with 6*His tags at C- ends are optimally synthesized and Pichia pastoris secretion expression vectors are constructed; (2) the recombinant vectors are transferred into Pichia pastoris hosts, yeast transformants with high level secretion expression are obtained, and recombinant scorpion insect Bj alpha IT proteins with a purity of over 95% are obtained by nickel affinity chromatography. A method for expressing insect neurotoxin Bj alpha IT genes with eukaryotic host Pichia pastoris is disclosed. The recombinant scorpion insect Bj alpha IT protein with high purity is quickly obtained by the nickel affinity chromatography, and the protein performs high bioactivity at both cellular and animal levels. The protein is of great value in the field of insect resistance, especially for breeding varieties of insect resistant plants, and has important significance for improving the yield of crops.

The invention provides a three flower fermentation original solution cosmetic product. The three flower fermentation original solution cosmetic product is prepared from a zymophyte solution with a lactobacillus plantarum concentration ranging from 10<6> to 10<8>CFU/ml, peach flower powder, Flores Chamomillae powder, rose flower powder, glucose, and water. The invention also discloses a preparationmethod of the three flower fermentation original solution cosmetic product. The preparation method comprises following steps: peach flower powder, Flores Chamomillae powder, rose flower powder, water, and zymophyte are mixed so as to obtain an initial fermentation system, the obtained fermentation solution is subjected to disinfection and centrifugation so as to obtain a supernatant, wherein thesupernatant is the three flower fermentation original solution cosmetic product. According to the preparation method, lactic acid bacteria is adopted for full plant fermentation of the three kinds offlowers, so that all plant functional components and the activities are maintained, active component loss caused by extraction method is avoided, and plant natural activity is maintained. The three flower fermentation original solution cosmetic product is rich in components, such as kaempferol, trifolin, vitamins, amino acids, rose polyphenols, and quercitrin, with excellent antioxidation activityand inhibition activity on tyrosinase.

The invention belongs to the technical field of biology. The invention relates to a polypeptide containing two dominant epitopes of canine brain natriuretic peptide (BNP). The immune effect is enhanced by coupling the polypeptide with KLH protein immune mice. The invention also provides a recombinant protein. The recombinant protein comprises two dominant epitopes of canine BNP, and in order to improve the yield of the recombinant protein in a prokaryotic expression system, escherichia coli preferred codons are adopted to convert the amino acid sequence of the recombinant protein into a corresponding nucleotide sequence, the nucleotide sequence is chemically synthesized, and a recombinant protein expression vector is constructed. The invention further relates to a phage library established by coupling polypeptide with KLH protein immune mice, a corresponding canine BNP single-chain antibody scfv sequence is obtained through panning and screening, the obtained scfv sequence is constructed into a complete mouse IgG1 antibody sequence expression vector, monoclonal antibodies are expressed through transient HEK293F cells, the monoclonal antibodies are purified, and the optimal monoclonal antibody pairing combination is determined through an immunofluorescence orthogonal experiment.

The invention relates to recombinant pectinase, a gene thereof, a recombinant vector, a preparation method and application. The gene at least comprises DNA fragment of one of a nucleotide sequence ofSEQ ID NO.1 in a sequence table, a nucleotide sequence having higher than 90% homology with the nucleotide sequence shown as the SEQ ID NO.1 and coding same biological functional protein and a nucleotide sequence hybridized with the nucleotide sequence shown as the SEQ ID NO.1 and coding same biological functional protein. The recombinant vector is further built according to the gene sequence, yeast is transformed, in this way, constitutive secretory expression of the recombinant pectinase in a high-density fermentation condition can be realized; active protein higher than 95% in purity and with high protein concentration can be obtained through simple nickel affinity purification; the active protein has high pectin hydrolyzing activity at low temperature, and a novel and efficient enzymeproduct can be provided for increasing juice yield of fruit juice.

The invention discloses a method for extracting nutritional ingredients such as free amino acids from waste cordyceps militaris cocoons, aiming at extracting various nutritional substances keeping natural activity from the waste cordyceps militaris cocoons, thus increasing the utilization rate of cordyceps militaris and the cocoons, and supplying for production for different types of nutritional healthcare products. The method is characterized by adopting a sequential extraction process of freeze-thawing, combined enzymatic hydrolysis, and water extraction and alcohol precipitation, wherein generally, two of the following enzymes are used in a matching manner, and the hydrolysis temperatures of the enzymes are as follows respectively: the hydrolysis temperature of papain is 45+/-2 DEG C; the hydrolysis temperatures of lysozyme, pectinase, amylase, pepsase and trypsin are 37+/-2 DEG C; the hydrolysis temperatures of bromelain, glucoamylase, monascus and cellulose are 35+/-2 DEG C; and the dosages of the enzymes are as follows respectively: in case of enzymatic hydrolysis for 1 kg of cordyceps militaris cocoon powder, 1 g of lysozyme for food, 2 g of amylase, 2 g of papain, 2 g of pepsase, 2 g of trypsin, 3 g of bromelain, 3 g of glucoamylase, 3 g of monascus, 3 g of cellulose and 4 g of pectinase are added.

The invention provides four-substance fermented water, and a preparation method and application thereof. The preparation method includes the steps that mixed powder of prepared rehmannia roots, angelica sinensis roots, szechuan lovage rhizome and radix paeoniae alba, water and zymophyte liquid are mixed to obtain an initial system, then fermentation is conducted to obtain fermented liquid, and thefermented liquid is sterilized and centrifuged to obtain a supernatant, namely the four-substance fermented water. The prepared rehmannia roots, the angelica sinensis roots, the szechuan lovage rhizome and the radix paeoniae alba are fermented by adopting two lactic acid bacteria, all functional components and activities of the prepared rehmannia roots, the angelica sinensis roots, the szechuan lovage rhizome and the radix paeoniae alba are reserved, and loss of active components caused by an extraction method is avoided.

The invention discloses a method for preparing gelsolin from Dendrorhynchus zhejiangensis, which is implemented by carrying out RNA (ribose nucleic acid) extraction, cDNA (complementary deoxyribonucleic acid) synthesis, enzyme digestion, connection and transformation, recombinant plasmid transformation, recombinant protein inducible expression, and recombinant protein separation and purification on the Dendrorhynchus zhejiangensis body wall, wherein after the cDNA is synthesized, a proper double-digestion primer is used for carrying out PCR (polymerase chain reaction) amplification, thereby obtaining a PCR product with the target segment; primers and a proper expression vector are subjected to double-digestion and connected, and are transformed onto a proper host; and the separation and purification are carried out under non-metamorphic conditions by an improved operation method using a Ni-NTAHis.BindResin kit, thereby saving the complex renaturation step, and being beneficial to keeping the natural activity of the recombinant protein and obtaining recombinant gelsolin with higher purity. The recombinant gelsolin prepared by the method disclosed by the invention can exist in a soluble form, and has an obvious inhibiting action on tumor cells; the concentration of the recombinant gelsolin is about 85%; and the method disclosed by the invention is scientific and reasonable, and is simple to implement and easy to popularize.