2236 results about "Cordyceps militaris" patented technology

The present invention relates to a method for extracting the purificatory cordycepin and the cordyceps amylase from the cordyceps militaris, belonging to the fields of medicines and health protection and chemical engineering. The cordyceps militaris powder is extracted and filtrated by a cooling seep method, the filter residue is mixed with ethanol, the supersonic wave assists to extract and offcenter, the supernatant has the processes of concentration and alcohol-precipitating, then the alcohol-precipitated supernatant has the processes of decompression, concentration and elution, the eluted liquid has the processes of concentration and crystallization and recrystallization using the n-butyl alcohol, at last the purificatory amylase is obtained after freezing and drying. The preparation progress of the present invention only uses water and ethanol as the solvent, thus reducing the pollution, the resin is capable of being reused a plurality of times with low cost. The present invention fully exploits the biological activity ingredients of the cordyceps militaris, realizes the coextraction of the cordycepin and the cordyceps amylase, guarantees the biological activities of the cordycepin and the cordyceps amylase, farthest increases the utilization rate of the raw material to the largest extent, reduces the manufacturing cost. The present invention is suitable for the large-scale industrialized manufacture.

The utility model provides a cordyceps militaris rice noodles product which is made from the castoff in the industrialized cultivation of the cordyceps militaris, and the product has a superior eating quality. The utility model provides a method of synthetically using the cordyceps militaris castoff, which is simple and good for the scale industrialized production.

The invention relates to a novel method for breeding cordyceps militaris by utilizing silkworm larvae. The method comprises the steps of taking live matured silkworm as the host, vaccinating cordyceps militaris strains through an injection method, and promoting silkworm to sleep, and the vaccination attainment rate is improved; after the method is adopted, the inactivation rate of the silkworm larvae reaches 90% to 100%, and the outgrowth rate of fruiting body reaches 85% to 100%; moreover, the water-solubility protein, polysaccharide, cordycepic acid, cordycepin and adenosine of the cordyceps militaris bred by the method are higher than those of cordyceps militaris bred by silkworm chrysalis in content. The novel method for breeding cordyceps militaris by utilizing silkworm larvae has the advantages of convenience in operation, low breeding cost, good experiment repeatability and wide application prospect, and is suitable for large-scale cultivation.

The invention discloses a method for extracting polysaccharide from a cordyceps militaris medium. The cordyceps militaris is crushed and mixed with water, is extracted in an enzyme liquor and an alkali liquor under 30 DEG C to 80 DEG C, and an obtained solution is centrifugalized. An insoluble substance is extracted repeatedly, and supernatants are combined. Amylase enzymolysis is performed on the obtained product under 50 DEG C to 75 DEG C. After centrifugation, a supernatant is condensed to be with a sugar content of 1.5 percent to 3.0 percent. The supernatant is added with ethanol and alcohol precipitation is performed on the obtained product under 0 DEG C to 4 DEG C, a product is obtained by drying the obtained product after centrifugation. After deproteinization and dialysis, crude cordyceps polysaccharide is obtained. A single enzyme method, a double enzyme method, a compound enzyme method and a supersonic wave enzyme method can be adopted, and protease, trypsin, etc. can be adopted. The method optimizes the technology route for extracting the cordyceps polysaccharide, enhances an obtaining rate and an extraction efficiency of the cordyceps polysaccharide. Extraction, separation and purification of the cordyceps polysaccharide are carried out effectively. The method provides a solution approach for the utilization of agricultural wastes, and provides a technology base for further exploitation of the cordyceps polysaccharide.

The invention belongs to the field of edible fungus culture and discloses a method for culturing cordyceps militaris by living silkworm chrysalises. The method comprises the following steps of: the preparation of stains, the selection of the silkworm chrysalises and disinfection treatment. The method is characterized by specifically comprising the following steps of: inoculating cordyceps militaris stains into silkworm chrysalis bodies through an injection method, wherein the stain dosage of each silkworm chrysalis body is 0.1-0.5ml; putting the inoculated silkworm chrysalises in sterile glass containers to culture the silkworm chrysalises in a culture room for 10-15 days at 18-25 DEG C, wherein 10-20 silkworm chrysalises are put in each container generally; increasing illumination from the twelfth day to promote sprouts to form; when little grass grows to 1cm, puncturing holes on sealing films so as to increase oxygen supply; meanwhile, watering the ground of the culture room to supplement the humidity; and after 20-30 days, when the height of stromata is 5-8 cm and the tops grow into wooden club shapes, collecting and drying. The method directly adopts the live silkworm chrysalises as culture carriers, can harvest the cordyceps militaris with the silkworm chrysalis bodies and changes the current situation that the cordyceps militaris cultured by rice culture media has no silkworm chrysalises.

The invention discloses a method for culturing selenium-enriched Cordyceps militaris, which is to increase the selenium content in a culture medium to 50mg/L by using organic selenium yeasts instead of inorganic seleninic acid and sodium selenite, which infect the growth of hyphae, so as to ensure the selenium content in the cultured selenium-enriched Cordyceps militaris reaches 35 to 50mg/L. In the invention, the organic selenium yeasts which exert the lest inhibition effect on the growth of the Cordyceps militaris are adopted to increase the selenium content in the culture medium to 50mg/l, the selenium content in the cultured selenium-enriched Cordyceps militaris reaches 35 to 50mg/kg, which is 2 times higher than that of the Cordyceps militaris cultured in a culture medium added with inorganic selenium, and the yield of Cordyceps militaris is high.

The invention discloses a method for preparing health food capsules by using cordyceps militaris and cocoon extracts, and aims to extract various nutrient substances with natural activities from the cordyceps militaris and cocoon to produce different types of nutrient health products, so that the utilization rate of the cordyceps militaris and cocoon is fully improved, and the market demand is met. The method is characterized by comprising the steps of extracting the nutrient substances from the cordyceps militaris and cocoon by performing freeze thawing, combined enzymolysis and aqueous alcohol precipitation sub-step extracting methods; adding a proper quantity of auxiliary materials into the nutrient substances to fully and evenly stir and sieve to prepare the particles; filling the particles into the capsules; wiping off residual powder adhered to the capsule shells; waxing; polishing; and sterilizing the capsules in a radiation mode after the capsules are packaged so as to obtain the finished product.

The invention provides a cordyceps militaris fruiting body culturing method used yellow meal worm pupae as host. The fruiting body is cultured by using cordyceps militaris link to infect yellow meal worm. Its features are as follows: yellow meal worm is yellow brown or chocolate brown; fruiting body is bar type and orange yellow. The culturing method includes bacteria species preparing, yellow meal worm inoculating infecting, and out grass. The formed product has multi nutrient content and effect active constituent, and uses as foods. The culturing method is simple, effective, and reliable. And the cost is low.

The invention relates to the field of microorganisms, in particular to a cultivation method of cordyceps militaris. The cultivation method can produce a large quantity of cordyceps militaris rhzomorph. The cultivation method comprises respectively preparing a slope culture medium, a liquid seed culture medium and a sporocarp cultivation culture medium; vaccinating cordyceps militaris cultures on the slope culture medium and cultivating at 23-25 DEG C for 6-8 days to obtain slope cultures; vaccinating the slope cultures on the liquid seed culture medium and conducting shake cultivation at 23-25 DEG C for 2-3 days at the speed of 250 circles/minute to obtain seed liquid; vaccinating the seed liquid on the sterilized sporocarp cultivation culture medium and conducting dark cultivation in a disinfected light-shielding culture room to obtain mycelium; conducting coloring cultivation on the mycelium and obtaining mycelium with buttons; and conducting sporocarp cultivation on the mycelium with the buttons to obtain the cordyceps militaris rhzomorph.

The invention discloses a health-care product for recuperating specific intrinsic constitution. The health-care product comprises the following raw materials in parts by weight: 1-8 parts of ginseng, 0.5-8 parts of cordyceps militaris, 1-9 parts of rhizoma polygonati and 1-8 parts of lilies; or 1-4 parts of longan aril, 1-5 parts of lotus seed, 1-2 parts of fingered citron, 1-2 parts of Chinese flowering quince, 1-6 parts of angelica dahurica, 1-6 parts of honeysuckle, 1-5 parts of red jujubes, 1-2 parts of mulberry leaves, 2-4 parts of Chinese yam, 2-6 parts of Chinese wolfberry, 1-4 parts of raspberry, 1-4 parts of euglenophyta, 1-2 parts of walnut kernels, 2-6 parts of horseradish tree leaves, 2-6 parts of snow lotus culture, 1-6 parts of male flowers of eucommia ulmoides, 1-8 parts of Peruvian ginseng powder, 1-4 parts of Acai, 1-4 parts of Noni puree, 1-4 parts of eucommia ulmoides seed oil, 1-2 parts of colla corii asini, 1-2 parts of hyacinth bean and 1-4 parts of Chia seed are added. According to the formation mechanism of the specific intrinsic constitution and the recuperating principle of symptoms, (artificially planted) ginseng capable of reinforcing vital energy and improving immunity, cordyceps militaris capable of complementing essence, qi and spirit of a human body, rhizoma polygonati capable of nourishing qi and yin, and the lilies capable of nourishing yin, clearing heat and promoting salivation are carefully selected from new food raw materials to be used as main raw materials, and the advantages of new resources are fully exerted.

The invention discloses a large-scale cultivation method and a quality detection method of cordyceps militaris fruit bodies, comprising the steps of: (1) screening spawn; (2) preparing a rice solid culture medium (40-55 parts of rice and 45-60 parts of nutrient solution in every 100 parts by weight) and killing bacteria; (3) preparing liquid spawn: activating the spawn, inoculating the spawn into a liquid culture medium, conducting static culture for 24 hours and performing suspension culture on a shaking table; (4) culturing fruit bodies: inoculating the liquid spawn on the solid culture medium for dark culture for 6 days at the temperature of 20 DEG C until the surface of the medium is covered by mycelium; placing the well grown mycelium under incandescence with light intensity of 100 to 300 lux, wherein during a primordium growth phase, illumination is performed for 20-24 hours per day at the temperature of 20-24 DEG C; and during a fruit bodies growth phase, illumination is performed for 8-12 hours per day at the temperature of 18-20 DEG C and the fruit bodies are harvested 5 to 8 days after mature. The cordyceps militaris fruit bodies cultured by the method have the advantages of high yield and high content of active ingredients. Batches of cordyceps militaris fruit bodies have fingerprints with a similarity value greater than 0.950, indicating stable and controllable quality.

The invention discloses a production process for wheat germ powder and belongs to the technical field of deep grain processing. Cordyceps militaris powder, walnut powder, pearl barley powder and bee pollen are adopted as auxiliary materials in the production process, wherein the mixture ratio of the cordyceps militaris powder to the walnut powder to the pearl barley powder to the bee pollen to the wheat germ powder in part by mass is 0.3-0.7 : 1.5-3 : 1.5-3 : 0.4-0.8 : 97-103. The production process comprises the steps as follows: filtering by a vibration sieve; deactivating enzyme and drying; conveying by a conveying belt; carrying out superfine grinding; sieving by an air jet sieve; carrying out three-dimensional mixing; carrying out microwave sterilization; and checking and packing. According to the production process, wheat germs are dried, subjected to enzyme deactivation, ground into ultrafine powder with fineness of 80-500 and subjected to microwave sterilization, so that the mouth feel of the wheat germ powder is improved, and the wheat germ powder with various wheat tastes and safe and reliable quality is produced.

The invention discloses a production technology for cordyceps militaris mycelium. The production technology mainly comprises the procedures of preparation of a test tube stock culture, culture medium preparation and culturing mode of a liquid strain, culture medium preparation and culturing mode of mycelium, and the like. The defects of the materials adopted by a culture medium are complex, the manufacturing cost is high and the liquid strain culturing condition is high in the prior art are changed by adjusting a formula and a technology according to the invention; after the mycelium is cultured and subjected to color transfer, the main components and pharmacology of the mycelium are more approached to those of the natural cordyceps militaris; and the production technology has the advantages of simple method, low comprehensive production cost, capability of performing large-scale production, and higher developing value.

The invention provides an extraction and purification method of cordycepin from Cordyceps militaris, and belongs to the deep-processing technical field of agricultural products. The method comprises the following steps: crushing Cordyceps militaris raw material, mixing the raw material with water at 1:20 raw material/water ratio, performing ultrasonic extraction, and performing centrifugal filtration on obtained extracting solution and pouring in a macroporous resin column at the adsorption flow of 1.8BV/hr; taking 15% methanol aqueous solution as an eluant to perform resin column elution, and decompressing and condensing obtained eluent at the temperature of 60 DEG C by a rotary evaporator to obtain Cordyceps militaris cordycepin concentrate. The method can help realize aqueous solution extraction of the cordycepin from the Cordyceps militaris raw material at low temperature, and the extraction rate reaches 85%; and polysaccharide and protein can be removed from the extract by macroporous resin separation and purification, thus the content of the cordycepin in the extract can reach 10%. The cordycepin in the Cordyceps militaris product has high activity, can be taken as a raw material for producing cordycepin monomer and can also be directly taken as an additive of health products and functional food, thus the cordycepin has wide market development prospect.

The invention discloses a method for preparing a composite beverage by fermenting Cordyceps militaris, which belongs to the nutrition and health beverage field. The composite beverage is prepared by using Cordyceps militaris, red date, Chinese wolfberry and honeysuckle as main raw materials, the method comprises the following steps: fermenting Cordyceps militaris to prepare fermentation liquor, respectively extracting red date, Chinese wolfberry and honeysuckle to obtain extract liquid, preparing the composite beverage according to the proportion that every 100mL beverage contains 40-60mL of Cordyceps militaris fermentation liquor, 10-20mL of Chinese wolfberry extract liquid, 2-8mL of honeysuckle extract and 25-40mL of red date extract liquid by adding water and seasoning auxiliary materials. The preparation method has the advantages of simple operation and short period. The prepared composite beverage by fermenting Cordyceps militaris has abundant nutrition and has auxiliary efficacies of raising human immunizing power, enhancing physical fitness, nourishing liver and improving eyesight, tonifying spleen and stomach, nourishing blood and tranquilizing mind, recuperating kidney and benefiting essence of life, aging resistance, antimutagenesis and antineoplastic.

The invention discloses a method for culturing cordyceps militaris bacterium. The method is as follows: finished bacterium powder is obtained after natural cordyceps militaris is subjected to separation and purification, culturing on a test tube slant, culturing of liquid first-grade seeds and second-grade seeds in oscillators, culturing of small-size seeds in a seed fermenter, concentration and spray-drying. The method for culturing cordyceps militaris bacterium disclosed by the invention has the beneficial effects that: the yield of cordyceps militaris can be increased and the price of cordyceps militaris can be reduced fundamentally; and the method plays a role in relieving pain of all peoples suffering from diseases and makes a wonder for traditional Chinese medicine treasure.

The invention relates to a method for extracting a composition containing nano-oligopeptide from cordyceps militaris as well as the composition extracted by the method. In the method, the cordyceps militaris is hydrolyzed by a complex enzymatic method to obtain the small molecular nano-oligopeptide. The invention is characterized in that the molecular weight of the nano-oligopeptide is mainly distributed within 100-1000D; and preferably the Gaussian distribution peak area percentage of the nano-oligopeptide the molecular weight of which is 100-500 D in the composition is more than 50%. The cordyceps militaris oligopeptide extract provided by the invention reserves other active ingredients such as cordycepin, ecdysone, adenosine, cordyceps polysaccharide, cordycepic acid, trace elements, vitamins and free amino acid. The invention further relates to an application of the composition containing the nano-oligopeptide extracted from the cordyceps militaris in preparing medicines, healthcare products and cosmetics.

The invention discloses a culture medium for cultivating cordyceps militaris, a preparation method of the culture medium, a culture method of cordyceps militaris and a preparation method of an oral cordyceps militaris tablet. The culture medium for cultivating cordyceps militaris is an aqueous solution and comprises 130 to 140g/L of dry silkworm chrysalis meal, 60 to 65g/L of rice flour, 1.2 to 1.5g/L of potassium dihydrogen phosphate and 0.8 to 1.2g/L of sodium dihydrogen phosphate. The culture method of cordyceps militaris comprises the following steps of inoculation, culture and harvest re-culture. The preparation method of the oral cordyceps militaris tablet comprises the following steps of drying, multiple cold sterilization treatment, crushing, wet granulation and tabletting. Cordyceps militaris cultured by the culture medium provided by the invention has nutrient content far higher than nutrient content of cordyceps militaris cultured by other culture methods, has a golden color, a luster and a heavy fragrance. In the preparation of the dried oral cordyceps militaris tablet, multiple cold sterilization treatment is carried out after drying. Through low-temperature superfine crushing, a cordyceps militaris spore wall breaking effect is guaranteed and a nutrition constituent loss is reduced.

The invention discloses a cordyceps militaris health-care beverage prepared by liquid submerged fermentation and a preparation method thereof. The preparation method comprises the following steps: sequentially performing culture activation, shake culture, seeding tank cultivation and fermentation tank cultivation on cordyceps militaris, then achieving high-temperature extraction of effective components and thromboplastin killing to obtain fermentation liquor, and finally conducting filtering, blending, sterilizing, subpackaging and other procedures to obtain the health-care beverage product. The cordyceps militaris health-care beverage is prepared by adopting the liquid submerged fermentation technology, is simple in process and free from influences of seasons and weather, meets the large-scale periodic production requirement, is short in producting cycle, low in cost, and excellent and stable in product quality, and has unique heavy fragrance, efficiency and properties of cordyceps militaris, thereby obtaining very wide market prospect.

The invention relates to a cordyceps militaris fruiting body with silkworm larvae as hosts and a cultivation method of the cordyceps militaris fruiting body. The cultivation method includes the steps: disinfecting and washing the cordyceps militaris fruiting body, cutting the cordyceps militaris fruiting body into sections, and inoculating the sections to a solid culture medium for cultivation to obtain a bacterial mass; inoculating the bacterial mass to a primary liquid strain culture medium for cultivation to obtain a primary liquid strain; diluting the primary liquid strain with sterile water to obtain primary liquid strain diluent; mixing pearlite and vermiculite, mixing the disinfected mixture with the primary liquid strain diluent to obtain cordyceps cultivation soil mixture; wiping the silkworm larvae at four to five instar with alcohol, using a disposable syringe to suck the primary liquid strain, and injecting into the silkworm larvae; and inserting the inoculated silkworm larvae into the cordyceps cultivation soil mixture, maintaining heads of the larvae up, and harvesting after cultivation to obtain the cordyceps militaris fruiting body with the silkworm larvae as the hosts. The head of the cordyceps militaris fruiting body is more than 2cm in length, and content of effective ingredients is high.

The invention disclosed a way to successively extract active ingredients from carpohole of north aweto. The invention adopts super critical CO2 to extract sterin substances, precipitating with alcohol to get nucleoside substances with cordycepin and Cordyceps polyose, separating with normal phase low pressure gel silica chromatography to get crude cordycipin, preparative reversed-phase high-performance liquid chromatography to get cordycepin crystal with high purity. The invention can not only successively extract sterin substances and Cordyceps polyose (CM-1) from the carpohole of north aweto, it can also get the cordycepin metamer; it is not applicable to small experiments but also applicable to industrial production. Since the producers can extract many substances by one treatment of carpohole, the invention can not only decrease the industrial cost but also make the biggest economic returns.

The invention discloses a feed formula of taking cordyceps militaris culture medium residue as a material, and a preparation method thereof, and solves the problems that the traditional feed is high in cost, and the quality of eggs cannot be well improved. The feed formula disclosed by the invention is prepared from cordyceps militaris culture medium residue powder, corn flour, wheat bran, bean pulp, salt, fish meal, bone powder, vitamin mixture, microelements, methionine and premix compound. The invention also provides the preparation method of the Chicken feed formula. In the components of the feed formula, industrial waste, namely residual solid culture medium residue after cordyceps militaris sporocarp is picked up is utilized; the industrial waste is fully recycled, so that the production cost of the feed is greatly reduced.

The invention discloses ampelopsis grossedentata selenium-enriched functional drink and a preparation method thereof and relates to natural health functional drink added with no preservative. The ampelopsis grossedentata selenium-enriched functional drink is prepared from the following components in percentage by weight: 10-80% of ampelopsis grossedentata extracting solution, 1-20% of cordyceps militaris extracting solution, 0.01-0.05% of vitamin C, 0.2-0.8% of beta-cyclodextrin, 0.01-0.05% of flavoured enzyme, (1-5)*10<-4>% of plant organic selenium and the balance of water. The prepared drink has the health effects of resisting to oxidation, protecting liver, helping digestion and improving immunity and can meet market diversification demand of drink.