9 results about "Silkworm larvae" patented technology

The application discloses a high-fiber silkworm larva feed containing protease and a preparation method thereof, and belongs to the technical field of enzyme engineering and feed for animal feeding in biological agriculture and related industries, and comprises a protease preparation-mulberry branch powder compound powder, mulberry branch powder, corn powder, soybean meal powder, citric acid, inorganic salt and guar gum. The application utilizes the easy-pulverization characteristics of fresh mulberry branches, adopts a pulverization and drying mode, shortens the drying time of the mulberry branches, and the artificial feed containing sufficient cellulose is beneficial to the feeding of silkworm larvae, promotes the peristalsis of the feed in the silkworm body, and is beneficial to meeting the feeding habits, main nutrition and physiological growth requirements of the silkworm; the proportion of the corn powder is increased to provide the silkworm with the required sugars and sugar compounds; the guar gum has good water retention performance, the silkworm larva feed after cooking is not prone to drying and hardening, and the use of the protease preparation effectively supplements the protease in the silkworm larvae, and promotes the growth and development of the silkworm larvae.

The present application belongs to the technical field of biological genetic engineering, and specifically discloses a method for excavating a gene associated with the body color trait of a tussock moth larva, constructing a tussock moth body color trait analysis population, and counting the body color trait phenotype value of the tussock moth body color trait analysis population; based on the Super-GBS technology, SNP markers are developed and typed at the whole genome level for tussock moth materials of the tussock moth body color trait analysis population, and a genetic linkage map is drawn; the body color trait is positioned by QTL using the genetic linkage map, the corresponding linkage group physical position of the body color trait is obtained, and the body color trait GWAS analysis is simultaneously carried out; according to the gene information obtained from the two methods, the associated regions are compared with a database and annotated, and candidate genes of the body color trait of the tussock moth larva are excavated. The present application obtains relevant gene information by the two methods of QTL and GWAS, compares the information with a database and annotates the information, and excavates candidate genes of the body color trait of the tussock moth, so that the candidate genes of the body color trait of the tussock moth at the whole genome level are effectively and accurately realized.

The present invention discloses a method for producing chimeric silk using silkworms by using Autographa californica nuclear polyhedrosis virus, and constructs a chimeric silk containing FibL-MaSp-c-polyA FibL The plasmid pFAST‑FibL‑MaSp‑c of the fragment is transformed into Escherichia coli containing AcBacmid DH10Ac, and recombinant AcBacmid‑FibL‑MaSp‑c is obtained by screening. The recombinant virus AcNPV‑FibL‑MaSp‑c is transfected into Sf‑9 cells to obtain the recombinant virus AcNPV‑FibL‑MaSp‑c, which is inoculated into 5-instar silkworm larvae, and the larvae are fed with cool-dried fresh mulberry leaves soaked or sprayed with antibiotic liquid for one day, and then fed with fresh mulberry leaves until they mature. The mature silkworms are fed with fresh mulberry leaves soaked or sprayed with ecdysteroid liquid; or the mature silkworms are directly sprayed with ecdysteroid liquid, and the mature silkworms are moved to a cocooning machine, cocoons are harvested after cocooning, and the cocoons are dried and reeled to obtain chimeric silk, thereby meeting the demand for diversity of silk protein in the preparation of various biomaterials.

The application provides a method for improving the growth and nutritional traits of domestic silkworms and application thereof, and relates to the technical field of domestic silkworm breeding. The application adds Lactobacillus rhamnosus to the domestic silkworms when the domestic silkworms are raised to the fourth instar, and the feeding dose of the Lactobacillus rhamnosus is 0.05-0.1 ml per silkworm; and / or the application adds Lactobacillus rhamnosus to the domestic silkworms when the domestic silkworms are raised to the fifth instar, and the feeding dose of the Lactobacillus rhamnosus is 0.1-0.2 ml per silkworm; the viable bacterial count of the Lactobacillus rhamnosus in each milliliter is 10 6 -10 8 CFU / mL. The application adds a certain dose of Lactobacillus rhamnosus to the domestic silkworms during the feeding process, so as to improve the viability of the domestic silkworms and the nutritional composition of the silkworm pupae, and the method has good application potential in the disease prevention of domestic silkworms and the screening and preparation of high-quality edible silkworm pupae.

The invention discloses a two-stage artificial breeding method for Harpactor fuscipes, and belongs to the technical field of biological control of agricultural pests, and the method comprises the following steps: breeding bean aphids by planting broad beans, and breeding newly hatched Harpactor fuscipes by using the bean aphids; then mulberry leaves are used for breeding silkworms, 1-3-year-old silkworm larvae are used for breeding 2-5-year-old sacharius fuscipes nymphs, the sacharius fuscipes nymphs and adult sacharius fuscipes are used for hunting lepidoptera pests in an orchard, and harm of the lepidoptera pests to fruit trees is reduced. The method utilizes low-value materials, is low in cost and simple to operate, can effectively reduce the population density of lepidoptera pests in the orchard and reduce fruit loss of the orchard, and has a wide application prospect in the field of orchard pest control.

An improved baculovirus expression system for enhanced heterologous protein expression in hosts selected from Bombyx mori larvae, pupae, primary cells, or established insect cell lines is disclosed. The expression system is derived from a wild-type strain of Bombyx mori nucleopolyhedrovirus (BmNPV) by deletion or inactivation of non-essential genes, of the BmNPV genome. The system includes novel transfer vectors designed for targeted gene modification and incorporation of a bacterial origin of replication and an insertion site for a gene of interest (GOI). This engineered bacmid maintains infectivity while offering enhanced genetic stability and significantly improved heterologous protein expression in insect cells, larvae, or pupae. Methods for constructing this bacmid via homologous recombination and using it for high-level protein production are also described.

This utility model discloses a silkworm feeding device, including a frame. A first hopper is fixedly connected to the upper end of the frame, and a second hopper is fixedly connected at an angle above and behind the first hopper. The first hopper contains a first variable frequency motor, a first circulating conveyor belt, a large brush roller, and a first infrared sensor. The second hopper contains a second variable frequency motor, a second circulating conveyor belt, a small brush roller, a spiral feeder, and a second infrared sensor. This silkworm feeding device is automated and features convenient operation and labor savings. During operation, precise feeding of silkworms can be achieved through detailed control of the variable frequency motor, small brush roller, large brush roller, first infrared sensor, and second infrared sensor.

The present application relates to the technical field of silkworm breeding, and particularly relates to a low-temperature environment adapted silkworm larva artificial feed and a preparation method thereof. The feed is a 1-3 age larva period exclusive formula, and comprises mulberry leaf powder, defatted soybean meal powder, corn flour, wheat bran, compound vitamins, compound inorganic salts, citric acid, potassium sorbate, choline and food-grade microbial inhibitors. Through the synergy of specific main material ratio and functional auxiliary materials, and the innovative process of mold steaming forming combined with freezing hardening and cutting, the feeding effect of the larva under extreme low-temperature conditions and the feed forming quality are significantly improved, and the industry problems of poor low-temperature adaptability and easy scattering of the feed in the prior art are solved.

The invention discloses a primer group for judging the cocooning time of bombyx mori after feeding pyriproxyfen-polluted mulberry leaves and application of the primer group, and a method for judging the cocooning time of bombyx mori by utilizing the primer group. The method comprises the following specific steps: (1) establishing a correlation between the relative transcription expression quantity of a normal silkworm chitin deacetylase 1 gene and the development time of five-instar silkworm larvae; (2) determining the CDA1 gene relative transcription expression quantity (T to be detected) of to-be-detected bombyx mori, calculating the relative transcription expression quantity (T normal) of the normal bombyx mori according to the development period of the to-be-detected bombyx mori by comparing the correlation obtained in the step (1), comparing the CDA1 gene transcription expression quantity of the to-be-detected bombyx mori with the CDA1 gene transcription expression quantity of the normal bombyx mori in the same period, and calculating the ratio R = T to be detected / T normal, and determining the cocooning time of the silkworms according to whether the ratio is greater than 1. According to the method, the accuracy, the efficiency and the operability of judging the cocooning time after the silkworm eats the mulberry leaves polluted by pyriproxyfen are improved.